Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,250,356 C→T 7.2% G110E (GGG→GAG)  cidB ← holin CidA anti‑holin regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,250,3560CT 7.2% 162.9 / 2.0 55G110E (GGG→GAG) cidBholin CidA anti‑holin regulator
Reads supporting (aligned to +/- strand):  ref base C (31/20);  new base T (2/2);  total (33/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GAGCACGCCGGTGAACATCACGAACACCGCGGTCAGTTCTGGCACCCCACCCAGGTCATGGGCCAGGGGCATGGCAAATGGCGTGGTGATCGAACGCGGCACCAGCGACAGGCTGGTTGCGCTGTCCAGCGCCAACAGGTGCGCCAGCCCCCAGGAGCTGGCGATCGAGGCACTGCTGCCGGCGACCATGCCCACCATCAGGGCCGGCCAATGACGCGCCAGCATGGCCCGCTGCTGCCAGATCGGCACCGCGAAGGCGACAGTCACCGGGCCGAGCACGCCCATCAACCAGTGG  >  NC_002947/5250208‑5250502
                                                                                                                                                    |                                                                                                                                                  
gAGCACGCCGGTGAACATCACGAACACCGCGGTCAGTTCTGGCACCCCACCCAGGTCATGGGCCAGGGGCATGGCAAATGGCGTGGTGATCGAACGCGGCACCAGCGACAGGCTGGTTGCGCTGTCCAGCGCCAACAGGTGCGCCAGcc                                                                                                                                                    >  2:353763/1‑149 (MQ=255)
      gCCGGTGAACATCACGAACACCGCGGTCAGTTCTGGCACCCCACCCAGGTCATGGGCCAGGGGCATGGCAAATGGCGTGGTGATCGAACGCGGCACCAGCGACAGGCTGGTTGCGCTGTCCAGCGCCAACAGGTGCGCCAGCCCCCAg                                                                                                                                               >  8:26673/1‑148 (MQ=255)
       ccGGTGAACATCACGAACACCGCGGTCAGTTCTGGCACCCCACCCAGGTCATGGGCCAGGGGCATGGCAAATGGCGTGGTGATCGAACGCGGCACCAGCGACAGGCTGGTTGCGCTGTCCAGCGCCAACAGGTGCGCCAGCCCCCAGGa                                                                                                                                             <  1:353763/149‑1 (MQ=255)
            gAACATCACGAACACCGCGGTCAGTTCTGGCACCCCACCCAGGTCATGGGCCAGGGGCATGGCAAATGGCGTGGTGATCGAACGCGGCACCAGCGACAGGCTGGTTGCGCTGTCCAGCGCCAACAGGTGCGCCAGCCCCCAGGAGCTgg                                                                                                                                        >  1:122624/1‑149 (MQ=255)
              aCATCACGAACACCGCGGTCAGTTCTGGCACCCCACCCAGGTCATGGGCCAGGGGCATGGCAAATGGCGTGGTGATCGAACGCGGCACCAGCGACAGGCTGGTTGCGCTGTCCAGCGCCAACAGGTGCGCCAGCTCCTGGGGGCTGGCg                                                                                                                                      >  6:360477/1‑149 (MQ=255)
               cATCACGAACACCGCGGTCAGTTCTGGCACCCCACCCAGGGCATGGGCCAGGGGCATGGCAAATGGCGTGGTGATCGAACGCGGCACCAGCGACAGGCTGGTTGCGCTGTCCAGCGCCAACAGGTGCGCCAGCTCCTGGGGGCTGGCgc                                                                                                                                     <  5:360477/149‑2 (MQ=255)
                aTCACGAACACCGCGGTCAGTTCTGGCACCCCACCCAGGTCATGGGCCAGGGGCATGGCAAATGGCGTGGTGATCGAACGCGGCACCAGCGACAGGCTGGTTGCGCTGTCCAGCGCCAACAGGTGCGCCAGCTCCTGGGGGCTGGCgca                                                                                                                                    >  8:509/1‑147 (MQ=255)
                 tCACGAACACCGCGGTCAGTTCTGGCACCCCACCCAGGTCATGGGCCAGGGGCATGGCAAATGGCGTGGTGATCGAACGCGGCACCAGCGACAGGCTGGTTGCGCTGTCCAGCGCCAACAGGTGCGCCAGCTCCTGGGGGCTGGCgcac                                                                                                                                   <  7:509/149‑4 (MQ=255)
                    cGAACACCGCGGTCAGTTCTGGCACCCCACCCAGGTCATGGGCCAGGGGCATGGCAAATGGCGTGGTGATCGAACGCGGCACCAGCGACAGGCTGGTTGCGCTGTCCAGCGCCAACAGGTGCGCCAGCCCCCAGGAGCTGGCGATCGAg                                                                                                                                <  6:152246/149‑1 (MQ=255)
                         acCGCGGACAGTTCTGGCACCCCACCCAGGTCATGGGCCAGGGGCATGGCAAATGGCGTGGTGATCGAACGCGGCACCAGCGACAGGCTGGTTGCGCTGTCCAGCGCCAACAGGTGCGCCAGCCCCCAGGAGCTGGCGATCGAGGCAct                                                                                                                           >  2:134957/1‑149 (MQ=255)
                          ccGCGGTCAGTTCTGGCACCCCACCCAGGTCATGGGCCAGGGGCATGGCAAATGGCGTGGTGATCGAACGCGGCACCAGCGACAGGCTGGTTGCGCTGTCCAGCGCCAACAGGTGCGCCAGCCCCCAGGAGCTGGCGATCGAGGCActg                                                                                                                          >  5:60888/1‑149 (MQ=255)
                              ggTCAGTTCTGGCACCCCACCCAGGTCATGGGCCAGGGGCATGGCAAATGGCGTGGTGATCGAACGCGGCACCAGCGACAGGCTGGTTGCGCTGTCCAGCGCCAACAGGTGCGCCAGCCCCCAGGAGCTGGCGATCGAGGCACtgctgc                                                                                                                      <  5:301649/149‑1 (MQ=255)
                                           accccacccAGGTCATGGGCCAGGGGCATGGCAAATGGCGTGGTGATCGAACGCGGCACCAGCGACAGGCTGGTTGCGCTGTCCAGCGCCAACAGGTGCGCCAGCCCCCAGGAGCTGGCGATCGAGGCACTGCTGCCGGCGACCATGcc                                                                                                         >  2:181003/1‑149 (MQ=255)
                                             cccacccaGGTCATGGGCCAGGGGCATGGCAAATGGCGTGGTGATCGAACGCGGCACCAGCGACAGGCTGGTTGCGCTGTCCAGCGCCAACAGGTGCGCCAGCCCCCAGGAGCTGGCGATCGAGGCACTGCTGCCGGCGACCATGCcca                                                                                                       <  6:60888/149‑1 (MQ=255)
                                                          cgggCAAGGGGCATGGAAAATGGCGTGGTGATCGAACGCGGCAACAGCGACAGGCTGGTTGCGCTTTCCAGATCAAACAGGTGCTCAAGCCCCAATGAGATGTCGATCGAGGCACTTCTGCCGGCGACCATGACCACCATCAGggccgg                                                                                          >  1:336931/2‑149 (MQ=255)
                                                             gCCAGGGGCATGGCAAATGGCGTGGTGATCGAACGCGGCACCAGCGACAGGCTGGTTGCGCTGTCCAGCGCCAACAGGTGCGCCAGCCCCCAGGAGCTGGCGATCGAGGCACTGCTGCCGGCGACCATGCCCACCATCAGggccggcc                                                                                        >  3:297937/1‑148 (MQ=255)
                                                              ccAGGGGCATGGCAAATGGCGTGGTGATCGAACGCGGCACCAGCGACAGGCTGGTTGCGCTGTCCAGCGCCAACAGGTGCGCCAGCCCCCAGGAGCTGGCGATCGAGGCACTGCTGCCGGCGACCATGCCCACCATCAGGGCCGGCCaa                                                                                      >  1:59173/1‑149 (MQ=255)
                                                               cAGGGGCATGGCAAATGGCGTGGTGATCGAACGCGGCACCAGCGACAGGCTGGTTGCGCTGTCCAGCGCCAACAGGTGCGCCAGCCCCCAGGAGCTGGCGATCGAGGCACTGCTGCCGGCGACCATGCCCACCATCAGGGCCGGCCAAt                                                                                     >  4:254518/1‑149 (MQ=255)
                                                                aGGGGCATGGCAAATGGCGTGGTGATCGAACGCGGCACCAGCGACAGGCTGGTTGCGCTGTCCAGCGCCAACAGGTGCGCCAGCCCCCAGGAGCTGGCGATCGAGGCACTGCTGCCGGCGACCATGCCCACCATCAGGGCCGGCCAATg                                                                                    <  2:59173/149‑1 (MQ=255)
                                                                 ggggCATGGCAAATGGCGTGGTGATCGAACGCGGCACCAGCGACAGGCTGGTTGCGCTGTCCAGCGCCAACAGGTGCGCCAGCCCCCAGGAGCTGGCGATCGAGGCACTGCTGCCGGCGACCATGCCCACCATCAGGGCCGGCCAATGa                                                                                   <  2:122624/149‑1 (MQ=255)
                                                                    gCATGGCAAATGGCGTGGTGATCGAACGCGGCACCAGCGACAGGCTGGTTGCGCTGTCCAGCGCCAACAGGTGCGCCAGCCCCCAGGAGCTGGCGATCGAGGCACTGCTGCCGGCGACCATGCCCACCATCAGGGCCGGCCAATGAcgc                                                                                >  6:34047/1‑149 (MQ=255)
                                                                         gCAAATGGCGTGGTGATCGAACGCGGCACCAGCGACAGGCTGGTTGCGCTGTCCAGCGCCAACAGGTGCGCCAGCCCCCAGGAGCTGGCGATCGAGGCACTGCTGCCGGCGACCATGCCCACCATCAGGGCCGGCCAATGACGCGCCAg                                                                           >  4:312012/1‑149 (MQ=255)
                                                                              tGGCGTGGTGATCGAACGCGGCACCAGCGACAGGCTGGTTGCGCTGTCCAGCGCCAACAGGTGCGCCAGCCCCCAGGAGCTGGCGATCGAGGCACTGCTGCCGGCGACCATGCCCACCATCAGGGCCGGCCAATGACGCGCCAGCATgg                                                                      <  5:34047/149‑1 (MQ=255)
                                                                                   tggtgATCGAACGCGGCACCAGCGACAGGCTGGTTGCGCTGTCCAGCGCCAACAGGTGCGCCAGCCCCCAGGAGCTGGCGATCGAGGCACTGCTGCCGGCGACCATGCCCACCATCAGGGCCGGCCAATGACGCGCCAGCATGGCCCgc                                                                 <  4:297937/149‑1 (MQ=255)
                                                                                            aaCGCGGCACCAGCGACAGGCTGGTTGCGCTGTCCAGCGCCAACAGGTGCGCCAGCCCCCAGGAGCTGGCGATCGAGGCACTGCTGCCGGCGACCATGCCCACCATCAGGGCCGGCCAATGACGCGCCAGCATGGCCCGCtgctgc                                                           >  3:180171/1‑146 (MQ=255)
                                                                                               gcgGCACCAGCGACAGGCTGGTTGCGCTGTCCAGCGCCAACAGGTGCGCCAGCCCCCAGGAGCTGGCGATCGAGGCACTGCTGCCGGCGACCATGCCCACCATCAGGGCCGGCCAATGACGCGCCAGCATGGCCCGCTGCTGCCAGATc                                                     <  7:26673/149‑1 (MQ=255)
                                                                                                           aCAGGCTGGTTGCGCTGTCCAGCGCCAACAGGTGCGCCAGCCCCCAGGAGCTGGCGATCGAGGCACTGCTGCCGGCGACCATGCCCACCATCAGGGCCGGCCAATGACGCGCCAGCATGGCCCGCTGCTGCCAGATCGGCACCGCGAAg                                         <  3:48888/149‑1 (MQ=255)
                                                                                                           aCAGGCTGGTTGCGCTGTCCAGCGCCAACAGGTGCGCCAGCCCCCAGGAGCTGGCGATCGAGGCACTGCTGCCGGCGACCATGCCCACCATCAGGGCCGGCCAATGACGCGCCAGCATGGCCCGCTGCTGCCAGATCGGCACCGCGAAg                                         <  3:254518/149‑1 (MQ=255)
                                                                                                             aGGCTGGTTGCGCTGTCCAGCGCCAACAGGTGCGCCAGCCCCCAGGAGCTGGCGATCGAGGCACTGCTGCCGGCGACCATGCCCACCATCAGGGCCGGCCAATGACGCGCCAGc                                                                          <  8:242695/114‑1 (MQ=255)
                                                                                                             aGGCTGGTTGCGCTGTCCAGCGCCAACAGGTGCGCCAGCCCCCAGGAGCTGGCGATCGAGGCACTGCTGCCGGCGACCATGCCCACCATCAGGGCCGGCCAATGACGCGCCAGc                                                                          >  7:242695/1‑114 (MQ=255)
                                                                                                             aGGCTGGTTGCGCTGTCCAGCGCCAACAGGTGCGCCAGCCCCCAGGAGCTGGCGATCGAGGCACTGCTGCCGGCGACCATGCCCACCATCAGGGCCGGCCAATGACGCGCCAGCATGGCCCGCTGCTGCCAGATCGGCACCGCGAAGGc                                       >  4:327399/1‑149 (MQ=255)
                                                                                                               gCTGGTTGCGCTGTCCAGCGCCAACAGGTGCGCCAGCCCCCAGGAGCTGGCGATCGAGGCACTGCTGCCGGCGACCATGCCCACCATCAGGGCCGGCCAATGACGCGCCAGCATGGCCCGCTGCTGCCAGAt                                                      >  1:80416/1‑132 (MQ=255)
                                                                                                               gCTGGTTGCGCTGTCCAGCGCCAACAGGTGCGCCAGCCCCCAGGAGCTGGCGATCGAGGCACTGCTGCCGGCGACCATGCCCACCATCAGGGCCGGCCAATGACGCGCCAGCATGGCCCGCTGCTGCCAGAt                                                      <  2:80416/132‑1 (MQ=255)
                                                                                                                ctgttggcgcTGGACAGCGCCAACAGGTGCGCCAGCCCCCAGGAGCTGGCGATCGAGGCACTGCTGCCGGCGACCATGCCCACCATCAGGGCCGGTCAATGACGCGCCAGCATGGCCCGCTGCTGCCAGATCGGCACCGCGAAGGCGa                                     >  4:319219/7‑148 (MQ=255)
                                                                                                                   gTTGCGCTGTCCAGCGCCAACAGGTGCGCCAGCCCCCAGGAGCTGGCGATCGAGGCACTGCTGCCGGCGACCATGCCCACCATCAGGGCCGGCCAATGACGCGCCAGCATGGCCCGCTGCTGCCAGATCGGCACCGCGAAGGCGACa                                   <  1:134957/147‑1 (MQ=255)
                                                                                                                   gTTGCGCTGTCCAGCGCCAACAGGTGCGCCAGCCCCCAGGAGCTGGCGATCGAGGCACTGCTGCCGGCGACCATGCCCACCATCAGGGCCGGCCAATGACGCGCCAGCATGGCCCGCTGCTGCCAGATCGGCACCGCGAAGGCGACAGt                                 >  4:190891/1‑149 (MQ=255)
                                                                                                                     tGCGCTGTCCAGCGCCAACAGGTGCGCCAGCCCCCAGGAGCTGGCGATCGAGGCACTGCTGCCGGCGACCATGCCCACCATCAGGGCCGGCCAATGACGCGCCAGCATGGCCCGCTGCTGCCAGATCGGCACCGCGAAGGCGACAGTCa                               >  3:123812/1‑149 (MQ=255)
                                                                                                                      gcgcTGTCCAGCGCCAACAGGTGCGCCAGCCCCCAGGAGCTGGCGATCGAGGCACTGCTGCCGGCGACCATGCCCACCATCAGGGCCGGCCAATGACGCGCCAGCATGGCCCGCTGCTGCCAGATCGGCACCGCGAAGGCGACAGTCAc                              >  7:307151/1‑149 (MQ=255)
                                                                                                                        gcTGTCCAGCGCCAACAGGTGCGCCAGCCCCCAGGAGCTGGCGATCGAGGCACTGCTGCCGGCGACCATGCCCACCATCAGGACCGGCCAATGACGCGCCAGCATGGCCCGCTGCTGCCAGATCGGCACCGCGAAGGCGACAGTCACCg                            >  7:19688/1‑149 (MQ=255)
                                                                                                                           gTCCAGCGCCAACAGGTGCGCCAGCCCCCAGGAGCTGGCGATCGAGGCACTGCTGCCGGCGACCATGCCCACCATCAGGGCCGGCCAATGACGCGCCAGCATGGCCCGCTGCTGCCa                                                         >  8:300344/1‑117 (MQ=255)
                                                                                                                           gTCCAGCGCCAACAGGTGCGCCAGCCCCCAGGAGCTGGCGATCGAGGCACTGCTGCCGGCGACCATGCCCACCATCAGGGCCGGCCAATGACGCGCCAGCATGGCCCGCTGCTGCCa                                                         <  7:300344/117‑1 (MQ=255)
                                                                                                                            tCCAGCGCCAACAGGTGCGCCAGCCCCCAGGAGCTGGCGATCGAGGCACTGCTGCCGGCGACCATGCCCACCATCAGGGCCGGCCAATGACGCGCCAGCATGGCCCGCTGCTGCCAGATCGGCACCGCGAAGGCGACAGTCACCGGGcc                        >  7:313821/1‑149 (MQ=255)
                                                                                                                              cAGCGCCAACAGGTGCGCCAGCCCCCAGGAGCTGGCGATCGAGGCACTGCTGCCGGCGACCATGCCCACCATCAGGGCCGGCCAATGACGCGCCAGCATGGCCCGCTGCTGCCAGATCGGCACCGCGAAGGCGACAGTCACCGGGCCGa                      <  1:181003/149‑1 (MQ=255)
                                                                                                                               aGCGCCAACAGGTGCGCCAGCCCCCAGGAGCTGGCGATCGAGGCACTGCTGCCGGCGACCATGCCCACCATCAGGGCCGGCCAATGACGCGCCAGCATGGCCCGCTGCTGCCAGATCGGCACCGCGAAGGCGACAGTCACCGGGCCGAg                     >  4:74858/1‑149 (MQ=255)
                                                                                                                               aGCGCCAACAGGTGCGCCAGCCCCCAGGAGCTGGCGATCGAGGCACTGCTGCCGGCGACCATGCCCACCATCAGGGCCGGCCAATGACGCGCCAGCATGGCCCGCTGCTGCCAGATCGGCACCGCGAAGGCGACAGTCACCGGGCCGAg                     >  8:33106/1‑149 (MQ=255)
                                                                                                                                gcgcCAACAGGTGCGCCAGCCCCCAGGAGCTGGCGATCGAGGCACTGCTGCCGGCGACCATGCCCACCATCAGGGCCGGCCAATGACGCGCCAGCATGGCCCGCTGCTGCCAGATCGGCACCGCGAAGGCGACAGTCACCGGGCCGAg                     >  2:111205/1‑148 (MQ=255)
                                                                                                                                  gcCAACAGGTGCGCCAGCCCCCAGGAGCTGGCGATCGAGGCACTGCTGCCGGCGACCATGCCCACCATCAGGGCCGGCCAATGACGCGCCAGCATGGCCCGCTGCTGCCAGATCGGCACCGCGAAGGCGACAGTCACCGGGCCGAGCAc                  >  7:278987/1‑149 (MQ=255)
                                                                                                                                   ccAACAGGTGCGCCAGCCCCCAGGAGCTGGCGATCGAGGCACTGCTGCCGGCGACCATGCCCACCATCAGGGCCGGCCAATGACGCGCCAGCATGGCCCGCTGCTGCCAGATCGGCACCGCGAAGGCGACAGTCACCGGGCCGAGCACg                 >  3:21216/1‑149 (MQ=255)
                                                                                                                                    cAACAGGTGCGCCAGCCCCCAGGAGCTGGCGATCGAGGCACTGCTGCCGGCGACCATGCCCACCATCAGGGCCGGCCAATGACGCGCCAGCATGGCCCGCTGCTGCCAGATCGGAACCGCGAAGGCGACAGTCACCGGGCCGAGCACGc                >  4:43781/1‑149 (MQ=255)
                                                                                                                                       cAGGTGCGCCAGCCCCCAGGAGCTGGCGATCGAGGCACTGCTGCCGGCGACCATGCCCACCATCAGGGCCGGCCAATGACGCGCCAGCATGGCCCGCTGCTGCCAGATCGGCACCGCGAAGGCGACAGTCACCGGGCCGAGCACGCCCa             <  4:251474/149‑1 (MQ=255)
                                                                                                                                       cAGGTGCGCCAGCCCCCAGGAGCTGGCGATCGAGGCACTGCTGCCGGCGACCATGCCCACCATCAGGGCCGGCCAATGACGCGCCAGCATGGCCCGCTGCTGCCAGATCGGCACCGCGAAGGCGACAGTCACCGGGCCGAGCACGCCCa             >  3:251474/1‑149 (MQ=255)
                                                                                                                                        aGGTGCGCCAGCCCCCAGGAGCTGGCGATCGAGGCACTGCTGCCGGCGACCATGCCCACCATCAGGGCCGGCCAATGACGCGCCAGCATGGCCCGCTGCTGCCAGATCGGCACCGCGAAGGCGACAGTCACCGGGCCGAGCACGCCCAt            >  6:18795/1‑149 (MQ=255)
                                                                                                                                               ccAGCCCCCAGGAGCTGGCGATCGAGGCACTGCTGCCGGCGACCATGCCCACCATCAGGGCCGGCCAATGACGCGCCAGCATGGCCCGCTGCTGCCAGATCGGCACCGCGAAGGCGACAGTCACCGGGCCGAGCACGCCCATCAACCAg     >  6:157555/1‑149 (MQ=255)
                                                                                                                                                 aGCCCCCAGGAGCTGGCGATCGAGGCACTGCTGCCGGCGACCATGCCCACCATCAGGGCCGGCCAATGACGCGCCAGCATGGCCCGCTGCTGCCAGATCGGCACCGCGAAGGCGACAGTCACCGGGCCGAGCACGCCCATCAACCAGTg   <  7:33106/149‑1 (MQ=255)
                                                                                                                                                  gCCCCCAGGAGCTGGCGATCGAGGCACTGCTGCCGGCGACCATGCCCACCATCAGGGCCGGCCAATGACGCGCCAGCATGGCCCGCTGCTGCCAGATCGGCACCGCGAAGGCGACAGTCACCGGGCCGAGCACGCCCATCAACCAGTgg  <  3:190891/149‑1 (MQ=255)
                                                                                                                                                  gCCCCCAGGAGCTGGCGATCGAGGCACTGCTGCCGGCGACCATGCCCACCATCAGGACCGGCCAATGACGCGCCAGCATGGCCCGCTGCTGCCAGATCGGCACCGCGAAGGCGACAGTCACCGGGCCGAGCACGCCCATCAACCAGTgg  <  8:19688/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
GAGCACGCCGGTGAACATCACGAACACCGCGGTCAGTTCTGGCACCCCACCCAGGTCATGGGCCAGGGGCATGGCAAATGGCGTGGTGATCGAACGCGGCACCAGCGACAGGCTGGTTGCGCTGTCCAGCGCCAACAGGTGCGCCAGCCCCCAGGAGCTGGCGATCGAGGCACTGCTGCCGGCGACCATGCCCACCATCAGGGCCGGCCAATGACGCGCCAGCATGGCCCGCTGCTGCCAGATCGGCACCGCGAAGGCGACAGTCACCGGGCCGAGCACGCCCATCAACCAGTGG  >  NC_002947/5250208‑5250502

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑