| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,250,363 | A→G | 6.9% | S108P (TCC→CCC) | cidB ← | holin CidA anti‑holin regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,250,363 | 0 | A | G | 6.9% | 166.7 / 2.9 | 58 | S108P (TCC→CCC) | cidB | holin CidA anti‑holin regulator |
| Reads supporting (aligned to +/- strand): ref base A (34/20); new base G (2/2); total (36/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.30e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.12e-01 | |||||||||||
CCGGTGAACATCACGAACACCGCGGTCAGTTCTGGCACCCCACCCAGGTCATGGGCCAGGGGCATGGCAAATGGCGTGGTGATCGAACGCGGCACCAGCGACAGGCTGGTTGCGCTGTCCAGCGCCAACAGGTGCGCCAGCCCCCAGGAGCTGGCGATCGAGGCACTGCTGCCGGCGACCATGCCCACCATCAGGGCCGGCCAATGACGCGCCAGCATGGCCCGCTGCTGCCAGATCGGCACCGCGAAGGCGACAGTCACCGGGCCGAGCACGCCCATCAACCAGTGGGTGTTGCGC > NC_002947/5250215‑5250511 | ccGGTGAACATCACGAACACCGCGGTCAGTTCTGGCACCCCACCCAGGTCATGGGCCAGGGGCATGGCAAATGGCGTGGTGATCGAACGCGGCACCAGCGACAGGCTGGTTGCGCTGTCCAGCGCCAACAGGTGCGCCAGCCCCCAGGa < 1:353763/149‑1 (MQ=255) gAACATCACGAACACCGCGGTCAGTTCTGGCACCCCACCCAGGTCATGGGCCAGGGGCATGGCAAATGGCGTGGTGATCGAACGCGGCACCAGCGACAGGCTGGTTGCGCTGTCCAGCGCCAACAGGTGCGCCAGCCCCCAGGAGCTgg > 1:122624/1‑149 (MQ=255) aCATCACGAACACCGCGGTCAGTTCTGGCACCCCACCCAGGTCATGGGCCAGGGGCATGGCAAATGGCGTGGTGATCGAACGCGGCACCAGCGACAGGCTGGTTGCGCTGTCCAGCGCCAACAGGTGCGCCAGCTCCTGGGGGCTGGCg > 6:360477/1‑149 (MQ=255) cATCACGAACACCGCGGTCAGTTCTGGCACCCCACCCAGGGCATGGGCCAGGGGCATGGCAAATGGCGTGGTGATCGAACGCGGCACCAGCGACAGGCTGGTTGCGCTGTCCAGCGCCAACAGGTGCGCCAGCTCCTGGGGGCTGGCgc < 5:360477/149‑2 (MQ=255) aTCACGAACACCGCGGTCAGTTCTGGCACCCCACCCAGGTCATGGGCCAGGGGCATGGCAAATGGCGTGGTGATCGAACGCGGCACCAGCGACAGGCTGGTTGCGCTGTCCAGCGCCAACAGGTGCGCCAGCTCCTGGGGGCTGGCgca > 8:509/1‑147 (MQ=255) tCACGAACACCGCGGTCAGTTCTGGCACCCCACCCAGGTCATGGGCCAGGGGCATGGCAAATGGCGTGGTGATCGAACGCGGCACCAGCGACAGGCTGGTTGCGCTGTCCAGCGCCAACAGGTGCGCCAGCTCCTGGGGGCTGGCgcac < 7:509/149‑4 (MQ=255) cGAACACCGCGGTCAGTTCTGGCACCCCACCCAGGTCATGGGCCAGGGGCATGGCAAATGGCGTGGTGATCGAACGCGGCACCAGCGACAGGCTGGTTGCGCTGTCCAGCGCCAACAGGTGCGCCAGCCCCCAGGAGCTGGCGATCGAg < 6:152246/149‑1 (MQ=255) acCGCGGACAGTTCTGGCACCCCACCCAGGTCATGGGCCAGGGGCATGGCAAATGGCGTGGTGATCGAACGCGGCACCAGCGACAGGCTGGTTGCGCTGTCCAGCGCCAACAGGTGCGCCAGCCCCCAGGAGCTGGCGATCGAGGCAct > 2:134957/1‑149 (MQ=255) ccGCGGTCAGTTCTGGCACCCCACCCAGGTCATGGGCCAGGGGCATGGCAAATGGCGTGGTGATCGAACGCGGCACCAGCGACAGGCTGGTTGCGCTGTCCAGCGCCAACAGGTGCGCCAGCCCCCAGGAGCTGGCGATCGAGGCActg > 5:60888/1‑149 (MQ=255) ggTCAGTTCTGGCACCCCACCCAGGTCATGGGCCAGGGGCATGGCAAATGGCGTGGTGATCGAACGCGGCACCAGCGACAGGCTGGTTGCGCTGTCCAGCGCCAACAGGTGCGCCAGCCCCCAGGAGCTGGCGATCGAGGCACtgctgc < 5:301649/149‑1 (MQ=255) accccacccAGGTCATGGGCCAGGGGCATGGCAAATGGCGTGGTGATCGAACGCGGCACCAGCGACAGGCTGGTTGCGCTGTCCAGCGCCAACAGGTGCGCCAGCCCCCAGGAGCTGGCGATCGAGGCACTGCTGCCGGCGACCATGcc > 2:181003/1‑149 (MQ=255) cccacccaGGTCATGGGCCAGGGGCATGGCAAATGGCGTGGTGATCGAACGCGGCACCAGCGACAGGCTGGTTGCGCTGTCCAGCGCCAACAGGTGCGCCAGCCCCCAGGAGCTGGCGATCGAGGCACTGCTGCCGGCGACCATGCcca < 6:60888/149‑1 (MQ=255) cgggCAAGGGGCATGGAAAATGGCGTGGTGATCGAACGCGGCAACAGCGACAGGCTGGTTGCGCTTTCCAGATCAAACAGGTGCTCAAGCCCCAATGAGATGTCGATCGAGGCACTTCTGCCGGCGACCATGACCACCATCAGggccgg > 1:336931/2‑149 (MQ=255) gCCAGGGGCATGGCAAATGGCGTGGTGATCGAACGCGGCACCAGCGACAGGCTGGTTGCGCTGTCCAGCGCCAACAGGTGCGCCAGCCCCCAGGAGCTGGCGATCGAGGCACTGCTGCCGGCGACCATGCCCACCATCAGggccggcc > 3:297937/1‑148 (MQ=255) ccAGGGGCATGGCAAATGGCGTGGTGATCGAACGCGGCACCAGCGACAGGCTGGTTGCGCTGTCCAGCGCCAACAGGTGCGCCAGCCCCCAGGAGCTGGCGATCGAGGCACTGCTGCCGGCGACCATGCCCACCATCAGGGCCGGCCaa > 1:59173/1‑149 (MQ=255) cAGGGGCATGGCAAATGGCGTGGTGATCGAACGCGGCACCAGCGACAGGCTGGTTGCGCTGTCCAGCGCCAACAGGTGCGCCAGCCCCCAGGAGCTGGCGATCGAGGCACTGCTGCCGGCGACCATGCCCACCATCAGGGCCGGCCAAt > 4:254518/1‑149 (MQ=255) aGGGGCATGGCAAATGGCGTGGTGATCGAACGCGGCACCAGCGACAGGCTGGTTGCGCTGTCCAGCGCCAACAGGTGCGCCAGCCCCCAGGAGCTGGCGATCGAGGCACTGCTGCCGGCGACCATGCCCACCATCAGGGCCGGCCAATg < 2:59173/149‑1 (MQ=255) ggggCATGGCAAATGGCGTGGTGATCGAACGCGGCACCAGCGACAGGCTGGTTGCGCTGTCCAGCGCCAACAGGTGCGCCAGCCCCCAGGAGCTGGCGATCGAGGCACTGCTGCCGGCGACCATGCCCACCATCAGGGCCGGCCAATGa < 2:122624/149‑1 (MQ=255) gCATGGCAAATGGCGTGGTGATCGAACGCGGCACCAGCGACAGGCTGGTTGCGCTGTCCAGCGCCAACAGGTGCGCCAGCCCCCAGGAGCTGGCGATCGAGGCACTGCTGCCGGCGACCATGCCCACCATCAGGGCCGGCCAATGAcgc > 6:34047/1‑149 (MQ=255) gCAAATGGCGTGGTGATCGAACGCGGCACCAGCGACAGGCTGGTTGCGCTGTCCAGCGCCAACAGGTGCGCCAGCCCCCAGGAGCTGGCGATCGAGGCACTGCTGCCGGCGACCATGCCCACCATCAGGGCCGGCCAATGACGCGCCAg > 4:312012/1‑149 (MQ=255) tGGCGTGGTGATCGAACGCGGCACCAGCGACAGGCTGGTTGCGCTGTCCAGCGCCAACAGGTGCGCCAGCCCCCAGGAGCTGGCGATCGAGGCACTGCTGCCGGCGACCATGCCCACCATCAGGGCCGGCCAATGACGCGCCAGCATgg < 5:34047/149‑1 (MQ=255) tggtgATCGAACGCGGCACCAGCGACAGGCTGGTTGCGCTGTCCAGCGCCAACAGGTGCGCCAGCCCCCAGGAGCTGGCGATCGAGGCACTGCTGCCGGCGACCATGCCCACCATCAGGGCCGGCCAATGACGCGCCAGCATGGCCCgc < 4:297937/149‑1 (MQ=255) aaCGCGGCACCAGCGACAGGCTGGTTGCGCTGTCCAGCGCCAACAGGTGCGCCAGCCCCCAGGAGCTGGCGATCGAGGCACTGCTGCCGGCGACCATGCCCACCATCAGGGCCGGCCAATGACGCGCCAGCATGGCCCGCtgctgc > 3:180171/1‑146 (MQ=255) gcgGCACCAGCGACAGGCTGGTTGCGCTGTCCAGCGCCAACAGGTGCGCCAGCCCCCAGGAGCTGGCGATCGAGGCACTGCTGCCGGCGACCATGCCCACCATCAGGGCCGGCCAATGACGCGCCAGCATGGCCCGCTGCTGCCAGATc < 7:26673/149‑1 (MQ=255) aCAGGCTGGTTGCGCTGTCCAGCGCCAACAGGTGCGCCAGCCCCCAGGAGCTGGCGATCGAGGCACTGCTGCCGGCGACCATGCCCACCATCAGGGCCGGCCAATGACGCGCCAGCATGGCCCGCTGCTGCCAGATCGGCACCGCGAAg < 3:48888/149‑1 (MQ=255) aCAGGCTGGTTGCGCTGTCCAGCGCCAACAGGTGCGCCAGCCCCCAGGAGCTGGCGATCGAGGCACTGCTGCCGGCGACCATGCCCACCATCAGGGCCGGCCAATGACGCGCCAGCATGGCCCGCTGCTGCCAGATCGGCACCGCGAAg < 3:254518/149‑1 (MQ=255) aGGCTGGTTGCGCTGTCCAGCGCCAACAGGTGCGCCAGCCCCCAGGAGCTGGCGATCGAGGCACTGCTGCCGGCGACCATGCCCACCATCAGGGCCGGCCAATGACGCGCCAGc < 8:242695/114‑1 (MQ=255) aGGCTGGTTGCGCTGTCCAGCGCCAACAGGTGCGCCAGCCCCCAGGAGCTGGCGATCGAGGCACTGCTGCCGGCGACCATGCCCACCATCAGGGCCGGCCAATGACGCGCCAGc > 7:242695/1‑114 (MQ=255) aGGCTGGTTGCGCTGTCCAGCGCCAACAGGTGCGCCAGCCCCCAGGAGCTGGCGATCGAGGCACTGCTGCCGGCGACCATGCCCACCATCAGGGCCGGCCAATGACGCGCCAGCATGGCCCGCTGCTGCCAGATCGGCACCGCGAAGGc > 4:327399/1‑149 (MQ=255) gCTGGTTGCGCTGTCCAGCGCCAACAGGTGCGCCAGCCCCCAGGAGCTGGCGATCGAGGCACTGCTGCCGGCGACCATGCCCACCATCAGGGCCGGCCAATGACGCGCCAGCATGGCCCGCTGCTGCCAGAt > 1:80416/1‑132 (MQ=255) gCTGGTTGCGCTGTCCAGCGCCAACAGGTGCGCCAGCCCCCAGGAGCTGGCGATCGAGGCACTGCTGCCGGCGACCATGCCCACCATCAGGGCCGGCCAATGACGCGCCAGCATGGCCCGCTGCTGCCAGAt < 2:80416/132‑1 (MQ=255) ctgttggcgcTGGACAGCGCCAACAGGTGCGCCAGCCCCCAGGAGCTGGCGATCGAGGCACTGCTGCCGGCGACCATGCCCACCATCAGGGCCGGTCAATGACGCGCCAGCATGGCCCGCTGCTGCCAGATCGGCACCGCGAAGGCGa > 4:319219/7‑148 (MQ=255) gTTGCGCTGTCCAGCGCCAACAGGTGCGCCAGCCCCCAGGAGCTGGCGATCGAGGCACTGCTGCCGGCGACCATGCCCACCATCAGGGCCGGCCAATGACGCGCCAGCATGGCCCGCTGCTGCCAGATCGGCACCGCGAAGGCGACa < 1:134957/147‑1 (MQ=255) gTTGCGCTGTCCAGCGCCAACAGGTGCGCCAGCCCCCAGGAGCTGGCGATCGAGGCACTGCTGCCGGCGACCATGCCCACCATCAGGGCCGGCCAATGACGCGCCAGCATGGCCCGCTGCTGCCAGATCGGCACCGCGAAGGCGACAGt > 4:190891/1‑149 (MQ=255) tGCGCTGTCCAGCGCCAACAGGTGCGCCAGCCCCCAGGAGCTGGCGATCGAGGCACTGCTGCCGGCGACCATGCCCACCATCAGGGCCGGCCAATGACGCGCCAGCATGGCCCGCTGCTGCCAGATCGGCACCGCGAAGGCGACAGTCa > 3:123812/1‑149 (MQ=255) gcgcTGTCCAGCGCCAACAGGTGCGCCAGCCCCCAGGAGCTGGCGATCGAGGCACTGCTGCCGGCGACCATGCCCACCATCAGGGCCGGCCAATGACGCGCCAGCATGGCCCGCTGCTGCCAGATCGGCACCGCGAAGGCGACAGTCAc > 7:307151/1‑149 (MQ=255) gcTGTCCAGCGCCAACAGGTGCGCCAGCCCCCAGGAGCTGGCGATCGAGGCACTGCTGCCGGCGACCATGCCCACCATCAGGACCGGCCAATGACGCGCCAGCATGGCCCGCTGCTGCCAGATCGGCACCGCGAAGGCGACAGTCACCg > 7:19688/1‑149 (MQ=255) gTCCAGCGCCAACAGGTGCGCCAGCCCCCAGGAGCTGGCGATCGAGGCACTGCTGCCGGCGACCATGCCCACCATCAGGGCCGGCCAATGACGCGCCAGCATGGCCCGCTGCTGCCa < 7:300344/117‑1 (MQ=255) gTCCAGCGCCAACAGGTGCGCCAGCCCCCAGGAGCTGGCGATCGAGGCACTGCTGCCGGCGACCATGCCCACCATCAGGGCCGGCCAATGACGCGCCAGCATGGCCCGCTGCTGCCa > 8:300344/1‑117 (MQ=255) tCCAGCGCCAACAGGTGCGCCAGCCCCCAGGAGCTGGCGATCGAGGCACTGCTGCCGGCGACCATGCCCACCATCAGGGCCGGCCAATGACGCGCCAGCATGGCCCGCTGCTGCCAGATCGGCACCGCGAAGGCGACAGTCACCGGGcc > 7:313821/1‑149 (MQ=255) cAGCGCCAACAGGTGCGCCAGCCCCCAGGAGCTGGCGATCGAGGCACTGCTGCCGGCGACCATGCCCACCATCAGGGCCGGCCAATGACGCGCCAGCATGGCCCGCTGCTGCCAGATCGGCACCGCGAAGGCGACAGTCACCGGGCCGa < 1:181003/149‑1 (MQ=255) aGCGCCAACAGGTGCGCCAGCCCCCAGGAGCTGGCGATCGAGGCACTGCTGCCGGCGACCATGCCCACCATCAGGGCCGGCCAATGACGCGCCAGCATGGCCCGCTGCTGCCAGATCGGCACCGCGAAGGCGACAGTCACCGGGCCGAg > 8:33106/1‑149 (MQ=255) aGCGCCAACAGGTGCGCCAGCCCCCAGGAGCTGGCGATCGAGGCACTGCTGCCGGCGACCATGCCCACCATCAGGGCCGGCCAATGACGCGCCAGCATGGCCCGCTGCTGCCAGATCGGCACCGCGAAGGCGACAGTCACCGGGCCGAg > 4:74858/1‑149 (MQ=255) gcgcCAACAGGTGCGCCAGCCCCCAGGAGCTGGCGATCGAGGCACTGCTGCCGGCGACCATGCCCACCATCAGGGCCGGCCAATGACGCGCCAGCATGGCCCGCTGCTGCCAGATCGGCACCGCGAAGGCGACAGTCACCGGGCCGAg > 2:111205/1‑148 (MQ=255) gcCAACAGGTGCGCCAGCCCCCAGGAGCTGGCGATCGAGGCACTGCTGCCGGCGACCATGCCCACCATCAGGGCCGGCCAATGACGCGCCAGCATGGCCCGCTGCTGCCAGATCGGCACCGCGAAGGCGACAGTCACCGGGCCGAGCAc > 7:278987/1‑149 (MQ=255) ccAACAGGTGCGCCAGCCCCCAGGAGCTGGCGATCGAGGCACTGCTGCCGGCGACCATGCCCACCATCAGGGCCGGCCAATGACGCGCCAGCATGGCCCGCTGCTGCCAGATCGGCACCGCGAAGGCGACAGTCACCGGGCCGAGCACg > 3:21216/1‑149 (MQ=255) cAACAGGTGCGCCAGCCCCCAGGAGCTGGCGATCGAGGCACTGCTGCCGGCGACCATGCCCACCATCAGGGCCGGCCAATGACGCGCCAGCATGGCCCGCTGCTGCCAGATCGGAACCGCGAAGGCGACAGTCACCGGGCCGAGCACGc > 4:43781/1‑149 (MQ=255) cAGGTGCGCCAGCCCCCAGGAGCTGGCGATCGAGGCACTGCTGCCGGCGACCATGCCCACCATCAGGGCCGGCCAATGACGCGCCAGCATGGCCCGCTGCTGCCAGATCGGCACCGCGAAGGCGACAGTCACCGGGCCGAGCACGCCCa > 3:251474/1‑149 (MQ=255) cAGGTGCGCCAGCCCCCAGGAGCTGGCGATCGAGGCACTGCTGCCGGCGACCATGCCCACCATCAGGGCCGGCCAATGACGCGCCAGCATGGCCCGCTGCTGCCAGATCGGCACCGCGAAGGCGACAGTCACCGGGCCGAGCACGCCCa < 4:251474/149‑1 (MQ=255) aGGTGCGCCAGCCCCCAGGAGCTGGCGATCGAGGCACTGCTGCCGGCGACCATGCCCACCATCAGGGCCGGCCAATGACGCGCCAGCATGGCCCGCTGCTGCCAGATCGGCACCGCGAAGGCGACAGTCACCGGGCCGAGCACGCCCAt > 6:18795/1‑149 (MQ=255) ccAGCCCCCAGGAGCTGGCGATCGAGGCACTGCTGCCGGCGACCATGCCCACCATCAGGGCCGGCCAATGACGCGCCAGCATGGCCCGCTGCTGCCAGATCGGCACCGCGAAGGCGACAGTCACCGGGCCGAGCACGCCCATCAACCAg > 6:157555/1‑149 (MQ=255) aGCCCCCAGGAGCTGGCGATCGAGGCACTGCTGCCGGCGACCATGCCCACCATCAGGGCCGGCCAATGACGCGCCAGCATGGCCCGCTGCTGCCAGATCGGCACCGCGAAGGCGACAGTCACCGGGCCGAGCACGCCCATCAACCAGTg < 7:33106/149‑1 (MQ=255) gCCCCCAGGAGCTGGCGATCGAGGCACTGCTGCCGGCGACCATGCCCACCATCAGGGCCGGCCAATGACGCGCCAGCATGGCCCGCTGCTGCCAGATCGGCACCGCGAAGGCGACAGTCACCGGGCCGAGCACGCCCATCAACCAGTgg < 3:190891/149‑1 (MQ=255) gCCCCCAGGAGCTGGCGATCGAGGCACTGCTGCCGGCGACCATGCCCACCATCAGGACCGGCCAATGACGCGCCAGCATGGCCCGCTGCTGCCAGATCGGCACCGCGAAGGCGACAGTCACCGGGCCGAGCACGCCCATCAACCAGTgg < 8:19688/149‑1 (MQ=255) cccAGGAGCTGGCGATCGAGGCACTGCTGCCGGCGACCATGCCCACCATCAGGGCCGGCCAATGACGCGCCAGCATGGCCCGCTGCTGCCAGATCg < 3:38429/96‑1 (MQ=255) cccAGGAGCTGGCGATCGAGGCACTGCTGCCGGCGACCATGCCCACCATCAGGGCCGGCCAATGACGCGCCAGCATGGCCCGCTGCTGCCAGATCg > 4:38429/1‑96 (MQ=255) cccAGGAGCTGGCGATCGAGGCACTGCTGCCGGCGACCATGCCCACCATCAGGGCCGGCCAATGACGCGCCAGCATGGCCCGCTGCTGCCAGATCGGCACCGCGAAGGCGACAGTCACCGGGCCGAGCACGCCCATCAACCAGTGGgtg > 8:213781/1‑149 (MQ=255) cccAGGAGCTGGCGATCGAGGCACTGCTGCCGGCGACCATGCCCACCATCAGGGCCGGCCAATGACGCGCCAGCATGGCCCGCTGCTGCCAGATCGGCACCGCGAAGGCGACAGTCACCGGGCCGAGCACGCCCATCAACCAGTGGgtg > 5:273509/1‑149 (MQ=255) cAGGAGCTGGCGATCGAGGCACTGCTGCCGGCGACCATGCCCACCATCAGGGCCGGCCAATGACGCGCCAGCATGGCCCGCTGCTGCCAGATCGGCACCGCGAAGGCGACAGTCACCGGGCCGAGCACGCCCATCAACCAGTGGGTGtt > 8:124341/1‑149 (MQ=255) aGCTGGCGATCGAGGCACTGCTGCCGGCGACCATGCCCACCATCAGGGCCGGCCAATGACGCGCCAGCATGGCCCGCTGCTGCCAGATCGGCACCGCGAAGGCGACAGTCACCGGGCCGAGCACGCCCATCAACCAGTGGGTGTTgcgc < 5:157555/149‑1 (MQ=255) | CCGGTGAACATCACGAACACCGCGGTCAGTTCTGGCACCCCACCCAGGTCATGGGCCAGGGGCATGGCAAATGGCGTGGTGATCGAACGCGGCACCAGCGACAGGCTGGTTGCGCTGTCCAGCGCCAACAGGTGCGCCAGCCCCCAGGAGCTGGCGATCGAGGCACTGCTGCCGGCGACCATGCCCACCATCAGGGCCGGCCAATGACGCGCCAGCATGGCCCGCTGCTGCCAGATCGGCACCGCGAAGGCGACAGTCACCGGGCCGAGCACGCCCATCAACCAGTGGGTGTTGCGC > NC_002947/5250215‑5250511 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |