| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,268,951 | T→A | 9.2% | intergenic (+19/+17) | PP_4643 → / ← radA | xanthine/uracil permease family protein/DNA repair enzyme |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,268,951 | 0 | T | A | 9.2% | 152.4 / 6.0 | 54 | intergenic (+19/+17) | PP_4643/radA | xanthine/uracil permease family protein/DNA repair enzyme |
| Reads supporting (aligned to +/- strand): ref base T (24/25); new base A (2/3); total (26/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.33e-01 | |||||||||||
TGATGGGGACTACTTCAAGGATGGCAAGCTGTTCGATGCCGAGGGTAACGAAGTGCCGATGCTGGAACTGGACGAGCACGGCAATGAAACGGTCAGGCGCACGGCCGTTGCCGAGCACTGACGGGTTGCA‑GGAGCGAGCTTGCTCGCTCCTGCACGCTATTCGAACAATGCATCCAGCGCCTGTTCCAGCCGGGTAACGGCAATCACCTGCAACCCCGCAGGCGCTTCTTTCGGCGCATTGCCCTTGGGCACGATGGCACGCTTGAAGCCGTGCTTGGCCGCTTCCTT > NC_002947/5268812‑5269099 | tGATGGGGACTACTTCAAGGATGGCAAGCTGTTCGATGCCGAGGGTAACGAAGTGCCGATGCTGGAACTGGACGAGCACGGCAATGAAACGGTCAGGCGCACGGCCGTTGCCGAGCACTGACGGGTTGCA‑GGAGCGAGCTTgctcgctc < 4:299476/149‑1 (MQ=255)tGATGGGGACTACTTCAAGGATGGCAAGCTGTTCGATGCCGAGGGTAACGAAGTGCCGATGCTGGAACTGGACGAGCACGGCAATGAAACGGTCAGGCGCACGGCCGTTGCCGAGCACTGACGGGTTGCA‑GGAGCGAGCAAgctcgctc < 4:273755/149‑1 (MQ=255)tGATGGGGACTACTTCAAGGATGGCAAGCTGTTCGATGCCGAGGGTAACGAAGTGCCGATGCTGGAACTGGACGAGCACGGCAATGAAACGGTCAGGCGCACGGCCGTTGCCGAGCACTGACGGGTTGCA‑GGAGCGAGCAAgctcgctc < 2:18455/149‑1 (MQ=255) gggACTACTTCAAGGATGGCAAGCTGTTCGATGCCGAGGGTAACGAAGTGCCGATGCTGGAACTGGACGAGCACGGCAATGAAACGGTCAGGCGCACGGCCGTTGCCGAGCACTGACGGGTTGCA‑GGAGCGAGCTTGCTCGCTCCTGc < 7:219880/148‑1 (MQ=255) gACTACTTCAAGGATGGCAAGCTGTTCGATGCCGAGGGTAACGAAGTGCCGATGCTGGAACTGTACGAGCACGGCAATGAAACGGTCAGGCGCACGGCCGTTGCCGAGCACTGACGGGTTGCA‑GGAGCGAGCTTGCTCGCTCCTGCACg < 6:148118/149‑1 (MQ=255) ttCAAGGATGGCAAGCTGTTCGATGCCGAGGGTAACGAAGTGCCGATGCTGGAACTGGACGAGCACGGCAATGAAACGGTCAGGCGCACGGCCGTTGCCGAGCACTGACGGGTTGCA‑GGAGCGAGCTTGCTCGCTCCTGCACGCTATTc > 8:27734/1‑149 (MQ=255) tCAAGGATGGCAAGCTGTTCGATGCCGAGGGTAACGAAGTGCCGATGCTGGAACTGGACGAGCACGGCAATGAAACGGTCAGGCGCACGGCCGTTGCCGAGCACTGACGGGTTGCA‑GGAGCGAGCTTGCTCGCTCCTGCACGCTATTc > 8:233921/1‑148 (MQ=255) gATGGCAAGCTGTTCGATGCCGAGGGTAACGAAGTGCCGATGCTGGAACTGGACGAGCACGGCAATGAAACGGTCAGGCGCACGGCCGTTGCCGAGCACTGACGGGTTGCA‑GGAGCGAGCTTgc < 4:279273/124‑1 (MQ=255) gATGGCAAGCTGTTCGATGCCGAGGGTAACGAAGTGCCGATGCTGGAACTGGACGAGCACGGCAATGAAACGGTCAGGCGCACGGCCGTTGCCGAGCACTGACGGGTTGCA‑GGAGCGAGCTTgc > 3:279273/1‑124 (MQ=255) gCTGTTCGATGCCGAGGGTAACGAAGTGCCGATGCTGGAACTGGACGAGCACGGCAATGAAACGGTCAGGCGCACGGCCGTTGCCGAGCACTGACGGGTTGCA‑GGAGCGAGCTTGCTCGCTCCTGCACGCTATTCGAACAATGCATCCa > 8:114701/1‑149 (MQ=255) gTAACGAAGTGCCGATGCTGGAACTGGACGAGCACGGCAATGAAACGGTCAGGCGCACGGCCGTTGCCGAGCACTGACGGGTTGCA‑GGAGCGAGCTTGCTCGCTCCTGCACGCTATTCGAACAATGCATCCAGCGCCTGTTCCAGCCgg > 1:111028/1‑149 (MQ=255) tAACGAAGTGCCGATGCTGGAACTGGACGAGCACGGCAATGAAACGGTCAGGCGCACGGCCGTTGCCGAGCACTGACGGGTTGCA‑GGAGCGAGCTTGCTCGCTCCTGCACGCTATTCGAACAATGCATCCAGCGCCTGTTCCAGCCggg > 8:96177/1‑149 (MQ=255) aaCGAAGTGCCGATGCTGGAACTGGACGAGCACGGCAATGAAACGGTCAGGCGCACGGTCGTTGCCGAGCACTGACGGGTTGCA‑GGAGCGAGCTTGCTCGCTCCTGCACGCTATTCGAACAATGCATCCAGCGCCTGtt > 7:6269/1‑139 (MQ=255) aaCGAAGTGCCGATGCTGGAACTGGACGAGCACGGCAATGAAACGGTCAGGCGCACGGTCGTTGCCGAGCACTGACGGGTTGCA‑GGAGCGAGCTTGCTCGCTCCTGCACGCTATTCGAACAATGCATCCAGCGCCTGtt < 8:6269/139‑1 (MQ=255) gAAGTGCCGATGCTGGAACTGGACGAGCACGGCAATGAAACGGTCAGGCGCACGGCCGTTGCCGAGCACTGACGGGTTGCA‑GGAGCGAGCTTGCTCGCTCCTGCACGCTATTCGAACAATGCATCCAGCGCCTGTTCCAGCCGGGTAAc > 5:167542/1‑149 (MQ=255) gAAGTGCCGATGCTGGAACTGGACGAGCACGGCAATGAAACGGTCAGGCGCACGGCCGTTGCCGAGCACTGACGGGTTGCA‑GGAGCGAGCTTGCTCGCTCCTGCACGCTATTCGAACAATGCATCCAGCGCCTGTTCCAGCCGGGTAAc < 2:111028/149‑1 (MQ=255) aGTGCCGATGCTGGAACTGGACGAGCACGGCAATGAAACGGTCAGGCGCACGGCCGTTGCCGAGCACTGACGGGTTGCA‑GGAGCGAGCTTGCTCGCTCCTGCACGCTATTCGAACAATGCATCCAGCGCCTGTTCCAGCCGGGTAACgg < 6:167542/149‑1 (MQ=255) aGTGCCGATGCTGGAACTGGACGAGCACGGCAATGAAACGGTCAGGCGCACGGCCGTTGCCGAGCACTGACGGGTTGCA‑GGAGCGAGCTTGCTCGCTCCTGCACGCTATTCGAACAATGCATCCAGCGCCTGTTCCAGCCGGGTAACgg < 1:140560/149‑1 (MQ=255) tGCCGATGCTGGAACTGGACGAGCACGGCAATGAAACGGTCAGGCGCACGGCCGTTGCCGAGCACTGACGGGTTGCA‑GGAGCGAGCTTGCTCGCTCCTGCACGCTATTCGAACAATGCATCCAGCGCCTGTTCCAGCCGGGTAACGGCa < 7:96177/149‑1 (MQ=255) gATGCTGGAACTGGACGAGCACGGCAATGAAACGGTCAGGCGCACGGCCGTTGCCGAGCACTGACGGGTTGCA‑GGAGCGAGCTTGCTCGCTCCTGCACGCTATTCGAACAATGCATCCAGCGCCTGTTCCAGCCGGGTAACGGCAATCa > 7:207373/1‑149 (MQ=255) tGGAACTGGACGAGCACGGCAATGAAACGGTCAGGCGCACGGCCGTTGCCGAGCACTGACGGGTTGCA‑GGAGCGAGCTTGCTCGCTCCTGCACGCTATTCGAACAATGCATCCAGCGCCTGTTCCAGCCGGGTAACGGCAATCACCTg > 3:340339/1‑148 (MQ=255) gAACTGGACGAGCACGGCAATGAAACGGTCAGGCGCACGGCCGTTGCCGAGCACTGACGGGTTGCA‑GGAGCGAGCTTGCTCGCTCCTGCACGCTATTCGAACAATGCATCCAGCGCCTGTTCCAGCCGGGTAACGGCAATCACCTGc < 7:114701/147‑1 (MQ=255) cTGGACGAGCACGGCAATGAAACGGTCAGGCGCACGGCCGTTGCCGAGCACTGACGGGTTGCA‑GGAGCGAGCTTGCTCGCTCCTGCACGCTATTCGAACAATGCATCCAGCGCCTGTTCCAGCCgg > 1:323334/1‑126 (MQ=255) cTGGACGAGCACGGCAATGAAACGGTCAGGCGCACGGCCGTTGCCGAGCACTGACGGGTTGCA‑GGAGCGAGCTTGCTCGCTCCTGCACGCTATTCGAACAATGCATCCAGCGCCTGTTCCAGCCgg < 2:323334/126‑1 (MQ=255) tGGACGAGCACGGCAATGAAACGGTCAGGCGCACGGCCGTTGCCGAGCACTGACGGGTTGCA‑GGAGCGAGCTTGCTCGCTCCTGCACGCTATTCGAACAATGCATCCAGCGCCTGTTCCAGCCGGGTAACGGCAATCAc > 8:275809/1‑139 (MQ=255) tGGACGAGCACGGCAATGAAACGGTCAGGCGCACGGCCGTTGCCGAGCACTGACGGGTTGCA‑GGAGCGAGCTTGCTCGCTCCTGCACGCTATTCGAACAATGCATCCAGCGCCTGTTCCAGCCGGGTAACGGCAATCAc < 7:275809/139‑1 (MQ=255) gACGAGCACGGCAATGAAACGGTCAGGCGCACGGCCGTTGCCGAGCACTGACGGGTTGCA‑GGAGCGAGCTTGCTCGCTCCTGCACGCTATTCGAACAATGCATCCAGCGCCTGTTCCAGCCGGGTAACGGCAATCACCTGCAACCCCg > 5:174992/1‑148 (MQ=255) gACGAGCACGGCAATGAAACGGTCAGGCGCACGGCCGTTGCCGAGCACTGACGGGTTGCA‑GGAGCGAGCTTGCTCGCTCCTGCACGCTATTCGAACAATGCATCCAGCGCCTGTTCCAGCCGGGTAACGGCAATCACCTGCAACCCCg > 6:44640/1‑148 (MQ=255) aCGAGCACGGCAATGAAACGGTCAGGCGCACGGCCGTTGCCGAGCACTGACGGGTTGCA‑GGAGCGAGCTTGCTCGCTCCTGCACGCTATTCGAACAATGCATCCAGCGCCTGTTCCAGCCGGGTAACGGCAATCACCTGCAACCCCg > 5:87522/1‑147 (MQ=255) aCGAGCACGGCAATGAAACGGTCAGGCGCACGGCCGTTGCCGAGCACTGACGGGTTGCA‑GGAGCGAGCTTGCTCGCTCCTGCACGCTATTCGAACAATGCATCCAGCGCCTGTTCCAGCCGGGTAACGGCAATCACCTGCAACCCCGCa < 8:207373/149‑1 (MQ=255) cGAGCACGGCAATGAAACGGTCAGGCGCACGGCCGTTGCCGAGCACTGACGGGTTGCA‑GGAGCGAGCTTGCTCGCTCCTGCACGCTATTCGAACAATGCATCCAGCGCCTGTTCCAGCCGGGTAACGGCAATCACCTGCAACCCCGCa < 6:87522/148‑1 (MQ=255) gAGCACGGCAATGAAACGGTCAGGCGCACGGCCGTTGCCGAGCACTGACGGGTTGCA‑GGAGCGAGCAAgctcgctc > 8:16144/1‑76 (MQ=255) gAGCACGGCAATGAAACGGTCAGGCGCACGGCCGTTGCCGAGCACTGACGGGTTGCA‑GGAGCGAGCAAgctcgctc < 7:16144/76‑1 (MQ=255) gCACGGCAATGAAACGGTCAGGCGCACGGCCGTTGCCGAGCACTGACGGGTTGCA‑GGAGCGAGCTTGCTCGCTCCTGCACGCTATTCGAACAATGCATCCAGCGCCTGTTCCAGCCGGGTAACGGCAATCACCTGCAACCCCGCAGgcg > 4:69029/1‑149 (MQ=255) cGGCAATGAAACGGTCAGGCGCACGGCCGTTGCCGAGCACTGACGGGTTGCA‑GGAGCGAGCTTGCTCGCTCCTGCACGCTATTCGAACAATGCATCCAGCGCCTGTTCCAGCCGGGTAACGGCAATCACCTGCAACCCCGCAGGCGctt > 6:23124/1‑149 (MQ=255) ggCAATGAAACGGTCAGGCGCACGGCCGTTGCCGAGCACTGACGGGTTGCA‑GGAGCGAGCTTGCTCGCTCCTGCACGCTATTCGAACAATGCATCCAGCGCCTGTTCCAGCCGGGTAACGGCAATCACCTGCAACCCCGCAGGCGcttc > 5:218358/1‑149 (MQ=255) aaTGAAACGGTCAGGCGCACGGCCGTTGCCGAGCACTGACGGGTTGCA‑GGAGCGAGCTTGCTCGCTCCTGCACGCTATTCGAACAATGCATCCAGCGCCTGTTCCAGCCGGGTAACGGCAATCACCTGCAACCCCGCAGGCGCTTCttt < 3:69029/149‑1 (MQ=255) tGAAACGGTCAGGCGCACGGCCGTTGCCGAGCACTGACGGGTTGCA‑GGAGCGAGCTTGCTCGCTCCTGCACGCTATTCGAACAATGCATCCAGCGCCTGTTCCAGCCGGGTAACGGCAATCACCTGCAACCCCGCAGGCGCTTCTTTCg < 7:233921/149‑1 (MQ=255) tCAGGCGCACGGCCGTTGCCGAGCACTGACGGGTTGCA‑GGAGCGAGCTTGCTCGCTCCTGCACGCTATTCGAACAATGCATCCAGCGCCTGTTCCAGCCGGGTAACGGCAATCACCTGCAACCCCGCAGGCGCTTCTTTCGGCGCATTg < 7:27734/149‑1 (MQ=255) aGGCGCACGGCCGTTGCCGAGCACTGACGGGTTGCA‑GGAGCGAGCTTGCTCGCTCCTGCACGCTATTCGAACAATGCATCCAGCGCCTGTTCCAGCCGGGTAACGGCAATCACCTGCAACCCCGCAGGCGCTTCTTTCGGCGCATTGcc < 6:174992/149‑1 (MQ=255) cgcACGGGCGTTGCCGAGCACTGACGGGTTGCA‑GGAGCGAGCTTGCTCGCTCCTGCACGCTATTCGAACAATGCATCCAGCGCCTGTTCCAGCCGGGTAACGGCAATCACCTGCAACCCCGCAGGCGCTTCTTTCGGCGCATTGCCCtt < 2:163032/149‑1 (MQ=255) gcACGGCCGTTGCCGAGCACTGACGGGTTGCA‑GGAGCGAGCTTGCTCGCTCCTGCACGCTATTCGAACAATGCATCCAGCGCCTGTTCCAGCCGGGTAACGGCAATCACCTGCAACCCCGCAGGCGCTTCTTTCGGCGCATTGCCCTTg < 6:218358/149‑1 (MQ=255) cACGGCCGTTGCCGAGCACTGACGGGTTGCA‑GGAGCGAGCTTGCTCGCTCCTGCACGCTATTCGAACAATGCATCCAGCGCCTGTTCCAGCCGGGTAACGGCAATCACCTGCAACCCCGCAGGCGCTTCTTTCGGCGCATTGCCCTTgg < 3:265323/149‑1 (MQ=255) ggCCGTTGCCGAGCACTGACGGGTTGCAGGGAGCGAGCTTGCTCGCTCCTGCACGCTATTCGAACAATGCATCCAGCGCCTGTTCCAGCCGGGTAACGGCAATCACCTGCAACCCCGCAGGCGCTTCTTTCGGCGCATTGCCCTTggg > 1:22094/1‑148 (MQ=255) ccGAGCACTGACGGGTTGCA‑GGAGCGAGCTTGCTCGCTCCTGCACGCTATTCGAACAATGCATCCAGCGCCTGTTCCAGCCGGGTAACGGCAATCACCTGCAACCCCGCAGGCGCTTCTTTCGGCGCATTGCCCTTGGGCACGATGGCa > 5:82607/1‑149 (MQ=255) cGAGCACTGACGGGTTGCA‑GGAGCGAGCTTGCTCGCTCCTGCACGCTATTCGAACAATGCATCCAGCGCCTGTTCCAGCCGGGTAACGGCAATCACCTGCAACCCCGCAGGCGCTTCTTTCGGCGCATTGCCCTTGGGCACGATGGCAc > 3:267873/1‑149 (MQ=255) aGCACTGACGGGTTGCA‑GGAGCGAGCTTGCTCGCTCCTGCACGCTATTCGAACAATGCATCCAGCGCCTGTTCCAGCCGGGTAACGGCAATCACCTGCAACCCCGCAGGCGCTTCTTTCGGCGCATTGCCCTTGGGCACGATGGCACGc > 1:195104/1‑149 (MQ=255) tGACGGGTTGCA‑GGAGCGAGCTTGCTCGCTCCTGCACGCTATTCGAACAATGCATCCAGCGCCTGTTCCAGCCGGGTAACGGCAATCACCTGCAACCCCGCAGGCGCTTCTTTCGGCGCATTGCCCTTGGGCACGATGGCACGCTTg > 5:1737/1‑147 (MQ=255) aCGGGTTGCA‑GGAGCGAGCTTGCTCGCTCCTGCACGCTATTCGAACAATGCATCCAGCGCCTGTTCCAGCCGGGTAACGGCAATCACCTGCAACCCCGCAGGCGCTTCTTTCGGCGCATTGCCCTTGGGCACGATGGCACGCTTGAAGc > 8:333851/1‑149 (MQ=255) gggTTGCA‑GGAGCGTTCTTGCTCGCTCCTGCACGCTATTCGAACTATGCATCCAGCGCCTGTTCCAGCCGGTTAACGGCAATCACCTGCAACCCCGCAGGCGCTTCTTTCGTCGCATTGCCCTTGGGCACGATGGCACGCTTGAAGCCg < 2:234792/149‑1 (MQ=255) ttGCA‑GGAGCGAGCTTGCTCGCTCCTGCACGCTATTCGAACAATGCATCCAGCGCCTGTTCCAGCCGTGTAACGGCAATCACCTGCAACCCCGCAGGCGCTTCTTTCGGCTCATTGCCCTTGGGCACGATGGCACGCTTGAAGCCGTGc < 5:44640/149‑1 (MQ=255) a‑GGAGCGAGCTTGCTCGCTCCTGCACGCTATTCGAACAATGCATCCAGCGCCTGTTCCAGCCGGGTAACTGCAATCACCTGCAACCCCGCAGGCGCTTCTTTCGGCGCATTGCCCTTGGGCACGATGGCACGCTTGAAGCCGTGCTTgg < 3:104621/149‑1 (MQ=255) gagcgagcAAGCTCGCTCCTGCACGCTATTCGAACAATGCATCCAGCGCCTGTTCCAGCCGGGTAACGGCAATCACCTGCAACCCCGCAGGCGCTTCTTTCGGCGCATTGCCCTTGGGCACGATGGCACGCTTGAAGCCGTGCTTGGcc > 3:259495/1‑149 (MQ=255) cTTGCTCGCTCCTGCACGCTATTCGAACAATGCATCCAGCGCCTGTTCCAGCCGGGTAACGGCAATCACCTGCAACCCCGCAGGCGCTTCTTTCGGCGCATTGCCCTTGGGCACGATGGCACGCTTGAAGCCGTGCTTGGCCGcttcct < 6:82607/149‑1 (MQ=255) ttGCTCGCTCCTGCACGCTATTCGAACAATGCATCCAGCGCCTGTTCCAGCCGGGTAACGGCAATCACCTGCAACCCCGCAGGCGCTTCTTTCGGCGCATTGCCCTTGGGCACGATGGCACGCTTGAAGCCGTGCTTGGCCGcttcctt > 3:72054/1‑149 (MQ=255) ttGCTCGCTCCTGCACGCTATTCGAACAATGCATCCAGCGCCTGTTCCAGCCGGGTAACGGCAATCACCTGCAACCCCGCAGGAGCTTCTTACGGCGCATTGCCCTTGGGCACGATGGCACGCTTGAAGCCGTGCTTGGCCGcttcctt > 7:159203/1‑149 (MQ=255) | TGATGGGGACTACTTCAAGGATGGCAAGCTGTTCGATGCCGAGGGTAACGAAGTGCCGATGCTGGAACTGGACGAGCACGGCAATGAAACGGTCAGGCGCACGGCCGTTGCCGAGCACTGACGGGTTGCA‑GGAGCGAGCTTGCTCGCTCCTGCACGCTATTCGAACAATGCATCCAGCGCCTGTTCCAGCCGGGTAACGGCAATCACCTGCAACCCCGCAGGCGCTTCTTTCGGCGCATTGCCCTTGGGCACGATGGCACGCTTGAAGCCGTGCTTGGCCGCTTCCTT > NC_002947/5268812‑5269099 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |