Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,319,943	G→T	6.1%	L59M (CTG→ATG)	PP_4681 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,319,943	0	G	T	6.1%	254.3 / 3.5	79	L59M (CTG→ATG)	PP_4681	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (35/39);  new base T (2/3);  total (37/42)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

AATTGATCAAAAGCTGCCAACAGGCGGCGTAGCTCACGACTTTGCTCCGGCCGGTCGGTAAACACGGTTTCGGCCATGACAAGGATGCTCGATGCATTGGGCAGCGGGTGCCCCAGCTCGATGATGATCTTCATGCGGGGCAGGAAGATCCATTGCAGCCACTGCTCAAAACTGAGGGTATC‑‑CACGGCGAACGGCACCGTACTGGCCAGTGCCTCGTCGCTTGGCGGCTCGTCATCCCACCAACCTTGCACCTGCAGCTCGCGCTCGATCAAGAGCAGGTGGTCGGCA  >  NC_002947/5319801‑5320088                                                                                                                                               |                                                                                                                                                    aaTTGATCAAAAGCTGCCAACAGGCGGCGTAGCTCACGACTTTGCTCCGGCCGGTCGGTAAACACGGTTTCGGCCATGACAAGGATGCTCGATGCATTGGGCAGCGGGTGCCCCAGCTCGATGATGATCTTCATGCGGGGCAgg                                                                                                                                                    >  6:26642/1‑144 (MQ=255)  aTTGATCAAAAGCTGCCAACAGGCGGCGTAGCTCACGACTTTGCTCCGGCCGGTCGGTAAACACGGTTTCGGCCATGACAAGGATGCTCGATGCATTGGGCAGCGGGTGCCCCAGCTCGATGATGATCTTCCTGCCCCGCATGAAGATc                                                                                                                                              <  7:112916/149‑1 (MQ=255)     gATCAAAAGCTGCCAACAGGCGGCGTAACTCACGACTTTGCTCCGGCCGGTCGGTAAACACGGTTTCGGCCATGACAAGGATGCTCGATGCATTGGGCAGCGGGTGCCCCAGCTCGATGATGATCTTCATGCGGGGCAGGAAGATCCAt                                                                                                                                           >  1:16527/1‑149 (MQ=255)      aTCAAAAGCTGCCAACAGGCGGCGTAGCTCACGACTTTGCTCCGGCCGGTCGGTAAACACGGTTTCGGCCATGACAAGGATGCTCGATGCATTGGGCAGCGGGTGCCCCAGCTCGATGATGATCTTCCTGCCCCGCATGAAGAt                                                                                                                                               <  7:219733/144‑1 (MQ=255)      aTCAAAAGCTGCCAACAGGCGGCGTAGCTCACGACTTTGCTCCGGCCGGTCGGTAAACACGGTTTCGGCCATGACAAGGATGCTCGATGCATTGGGCAGCGGGTGCCCCAGCTCGATGATGATCTTCCTGCCCCGCATGAAGAt                                                                                                                                               >  8:219733/1‑144 (MQ=255)      aTCAAAAGCTGCCAACAGGCGGCGTAGCTCACGACTTTGCTCCGGCCGGTCGGTAAACACGGTTTCGGCCATGACAAGGATGCTCGATGCATTGGGCAGCGGGTGCCCCAGCTCGATGATGATCTTCCTGCCCCGCATGAAGATcatc                                                                                                                                           <  1:278924/148‑4 (MQ=255)       tCAAAAGCTGCCAACAGGCGGCGTAGCTCACGACTTTGCTCCGGCCGGTCGGTAAACACGGTTTCGGCCATGACAAGGATGCTCGATGCATTGGGCAGCGGGTGCCCCAGCTCGATGATGATCTTCATGCGGGGCAGGAAGATCCATTg                                                                                                                                         <  2:82484/149‑1 (MQ=255)         aaaaGCTGCCAACAGGCGGCGTAGCTCACGACTTTGCTCCGGCCTGTCGGTAAACACGGTTTCGGCCATGACAAGGATGCTCGATGCATTGGGCAGCGGGTGCCCCAGCTCGATGATGATCTTCATGCGGGGCAGGAAGATCCATTGc                                                                                                                                        <  8:164509/148‑1 (MQ=255)          aaaGCTGCCAACAGGCGGCGTAGCTCACGACTTTGCTCCGGCCGGTCGGTAAACACGGTTTCGGCCATGACAAGGATGCTCGATGCATTGGGCAGCGGGTGCCCCAGCTCGATGATGATCTTCATGCGGGGCAGGAAGATCCATTGCAg                                                                                                                                      >  6:201713/1‑149 (MQ=255)           aaGCTGCCAACAGGCGGCGTAGCTCACGACTTTGCTCCGGCCGGTCGGTAAACACGGTTTCGGCCATGACAAGGATGCTCGATGCATTGGGCAGCGGGTGCCCCAGCTCGATGATGATCTTCATGCGGGGCAGGAAGATCCATTGCAGc                                                                                                                                     <  2:16527/149‑1 (MQ=255)           aaGCTGCCAACAGGCGGCGTAGCTCACGACTTTGCTCCGGCCGGTCGGTAAACACGGTTTCGGCCATGACAAGGATGCTCGATGCATTGGGCAGCGGGTGCCCCAGCTCGATGATGATCTTCATGCGGGGCAGGAAGATCCATTGCAGc                                                                                                                                     >  4:235519/1‑149 (MQ=255)               tGCCAACAGGCGGCGTAGCTCACGACTTTGCTCCGGCCGGTCGGTAAACACGGTTTCGGCCATGACAAGGATGCTCGATGCATTGGGCAGCGGGTGCCCCAGCTCGATGATGATCTTCATGCGGGGCAGGAAGATCCATTGCAGCCACt                                                                                                                                 >  4:192310/1‑149 (MQ=255)                 ccAACAGGCGGCGTAGCTCACGACTTTGCTCCGGCCGGTCGGTAAACACGGTTTCGGCCATGACAAGGATGCTCGATGCATTGGGCAGCGGGTGCCCCAGCTCGATGATGATCTTCATGCGGGGCAGGAAGATCCATTGCAGCCACTGc                                                                                                                               >  3:190623/1‑149 (MQ=255)                  cAACAGGCGGCGTAGCTCACGACTTTGCTCCGGCCGGTCGGTAAACACGGTTTCGGCCATGACAAGGATGCTCGATGCATTGGGCAGCGGGTGCCCCAGCTCGATGATGATCTTCATGCGGGGCAGGAAGATCCATTGCAGCCACTGCt                                                                                                                              <  4:190623/149‑1 (MQ=255)                       ggcggcGTAGCTCACGACTTTGCTCCGGCCGGTCGGTAAACACGGTTTCGGCCATGACAAGGATGCTCGATGCATTGGGCAGCGGGTGCCCCAGCTCGATGATGATCTTCATGCGGGGCAGGAAGATCCATTGCAGCCACTGCTCaaaa                                                                                                                         <  8:229723/149‑1 (MQ=255)                           gcgTAGCTCACGACTTTGCTCCGGCCGGTCGGTAAACACGGTTTCGGCCATGACAAGGATGCTCGATGCATTGGGCAGCGGGTGCCCCAGCTCGATGATGATCTTCATGCGGGGCAGGAAGATCCATTGCAGCCACTGCTCAAAACTGa                                                                                                                     <  3:235519/149‑1 (MQ=255)                            cgTAGCTCACGACTTTGCTCCGGCCGGTCGGTAAACACGGTTTCGGCCATGACAAGGATGCTCGATGCATTGGGCAGCGGGTGCCCCAGCTCGATGATGATCTTCATGCGGGGCAGGAAGATCCATTGCAGCCACTGCTCAAAACTGAg                                                                                                                    <  1:143859/149‑1 (MQ=255)                            cgTAGCTCACGACTTTGCTCCGGCCGGTCGGTAAACACGGTTTCGGCCATGACAAGGATGCTCGATGCATTGGGCAGCGGGTGCCCCAGCTCGATGATGATCTTCATGCGGGGCAGGAAGATCCATTGCAGCCACTGCTCAAAACTGAg                                                                                                                    <  5:201713/149‑1 (MQ=255)                             gTAGCTCACGACTTTGCTCCGGCCGGTCGGTAAACACGGTTTCGGCCATGACAAGGATGCTCGATGCATTGGGCAGCGGGTGCCCCAGCTCGATGATGATCTTCATGCGGGGCAGGAAGATCCATTGCAGCCACTGCTCAAAACTGAgg                                                                                                                   <  3:89354/149‑1 (MQ=255)                                 cTCACGACTTTGCTCCGGCCGGTCGGTAAACACGGTTTCGGCCATGACAAGGATGCTCGATGCATTGGGCAGCGGGTGCCCCAGCTCGATGATGATCTTCATGCGGGGCAGGAAGATCCATTGCAGCCACTGCTCAAAACTGAGGGTAt                                                                                                               <  5:174982/149‑1 (MQ=255)                                  tCACGACTTTGCTCCGGCCGGTCGGTAAACACGGTTTCGGCCATGACAAGGATGCTCGATGCATTGGGCAGCGGGTGCCCCAGCTCGATGATGATCTTCATGCGGGGCAGGAAGATCCATTGCAGCCACTGCTCAAAACTGAGGGTATc                                                                                                              >  2:17285/1‑149 (MQ=255)                                   aaCGACTTTGCTCCGGCCGGTCGGTAAACACGGTTTCGGCCATGACAAGGATGCTCGATGCATTGGGCAGCGGGTGCCCCAGCTCGATGATGATCTTCATGCGGTGCATCAAGATCCATTGCAGCCACTGCTCAAAACTGATGGAATc                                                                                                              >  6:98437/2‑148 (MQ=255)                                    aCGACTTTGCTCCGGCCGGTCGGTAAACACGGTTTCGGCCATGACAAGGATGCTCGATGCATTGGGCAGCGGGTGCCCCAGCTCGATGATGATCTTCATGCGGGGCAGGAAGATCCATTGCa                                                                                                                                       >  2:201958/1‑122 (MQ=255)                                    aCGACTTTGCTCCGGCCGGTCGGTAAACACGGTTTCGGCCATGACAAGGATGCTCGATGCATTGGGCAGCGGGTGCCCCAGCTCGATGATGATCTTCATGCGGGGCAGGAAGATCCATTGCa                                                                                                                                       <  1:201958/122‑1 (MQ=255)                                    aCGACTTTGCTCCGGCCGGTCGGTAAACACGGTTTCGGCCATGACAAGGATGCTCGATGCATTGGGCAGCGGGTGCCCCAGCTCGATGATGATCTTCATGCGGGGCAGGAAGATCCATTGCAGCCACTGCTCAAAACTGAGGGTATc‑‑c                                                                                                           <  8:16306/148‑1 (MQ=255)                                      gACTTTGCTCCGGCCGGTCGGTAAACACGGTTTCGGCCATGACAAGGATGCTCGATGCATTGGGCAGCGGGTGCCCCAGCTCGATGATGATCTTCATGCGGGGCAGGAAGATCCATTGCAGCCAc                                                                                                                                  <  7:321765/125‑1 (MQ=255)                                      gACTTTGCTCCGGCCGGTCGGTAAACACGGTTTCGGCCATGACAAGGATGCTCGATGCATTGGGCAGCGGGTGCCCCAGCTCGATGATGATCTTCATGCGGGGCAGGAAGATCCATTGCAGCCAc                                                                                                                                  >  8:321765/1‑125 (MQ=255)                                             cTCCGGCCGGTCGGTAAACACGGTTTCGGCCATGACAAGGATGCTCGATGCATTGGGCAGCGGGTGCCCCAGCTCGATGATGATCTTCATGCGGGGCAGGAAGATCCATTGCAGCCACTGCTCAAAACTGAGGGTATC‑‑CACGGCGAACg                                                                                                 <  5:98437/149‑1 (MQ=255)                                               ccggccggTCGGTAAACACGGTTTCGGCCATGACAAGGATGCTCGATGCATTGGGCAGCGGGTGCCCCAGCTCGATGATGATCTTCATGCGGGGCAGGAAGATCCATTGCAGCCACTGCTCAAAACTGAGGGTATC‑‑CACGGCGAACgg                                                                                                >  4:165680/1‑148 (MQ=255)                                                       tcggtAAACACGGTTTCGGCCATGACAAGGATGCTCGATGCATTGGGCAGCGGGTGCCCCAGCTCGATGATGATCTTCATGCGGGGCAGGAAGATCCATTGCAGCCACTGCTCAAAACTGAGGGTATC‑‑CACGGCGAACg                                                                                                 >  5:215076/1‑139 (MQ=255)                                                       tcggtAAACACGGTTTCGGCCATGACAAGGATGCTCGATGCATTGGGCAGCGGGTGCCCCAGCTCGATGATGATCTTCATGCGGGGCAGGAAGATCCATTGCAGCCACTGCTCAAAACTGAGGGTATC‑‑CACGGCGAACg                                                                                                 <  6:215076/139‑1 (MQ=255)                                                         ggtAAACACGGTTTCGGCCATGACAAGGATGCTCGATGCATTGGGCAGCGGGTGCCCCAGCTCGATGATGATCTTCATGCGGGGCAGGAAGATCCATTGCAGCCACTGCTCAAAACTGa                                                                                                                     >  8:145689/1‑119 (MQ=255)                                                         ggtAAACACGGTTTCGGCCATGACAAGGATGCTCGATGCATTGGGCAGCGGGTGCCCCAGCTCGATGATGATCTTCATGCGGGGCAGGAAGATCCATTGCAGCCACTGCTCAAAACTGa                                                                                                                     <  7:145689/119‑1 (MQ=255)                                                              acacGGTTTCGGCCATGACAAGGATGCTCGATGCATTGGGCAGCGGGTGCCCCAGCTCGATGATGATCTTCATGCGGGGCAGGAAGATCCATTGCAGCCACTGCTCAAAACTGAGGGTATC‑‑CACGGCGAACGGCACCGTACTGGCCAGt                                                                                <  6:13086/149‑1 (MQ=255)                                                               cacGGTTTCGGCCATGACAAGGATTCTCGATGCATTGGGCAGCGGGTGCCCCAGCTCGATGATGATCTTCATGCGGGGCAGGAAGATCCATTGCAGCCACTGCTCAAAACTGAGGGTATC‑‑CACGGCGAACGGCACCGTACTGGCCAGTg                                                                               <  5:26642/149‑1 (MQ=255)                                                               cacGGTTTCGGCCATGACAAGGATGCTCGATGCATTGGGCAGCGGGTGCCCCAGCTCGATGATGATCTTCATGCGGGGCAGGAAGATCCATTGCAGCCACTGCTCAAAACTGAGGGTATC‑‑CACGGCGAACGGCACCGTACTGGCCAGTg                                                                               >  3:296348/1‑149 (MQ=255)                                                                 cGGTTTCGGCCATGACAAGGATGCTCGATGCATTGGGCAGCGGGTGCCCCAGCTCGATGATGATCTTCATGCGGGGCAGGAAGATCCATTGCAGCCACTGCTCAAAACTGAGGGTATC‑‑CACGGCGAACGGCACCGTACTGGCCAGTGcc                                                                             <  3:192310/149‑1 (MQ=255)                                                                       cGGCCATGACAAGGATGCTCGATGCATTGGGCAGCGGGTGCCCCAGCTCGATGATGATCTTCATGCGGGGCAGGAAGATCCATTGCAGCCACTGCTCAAAACTGAGGGTATC‑‑CACGGCGAACGGCACCGTACTGGCCAGTGCCtcgtcg                                                                       >  1:85554/1‑149 (MQ=255)                                                                          ccATGACAAGGATGCTCGATGCATTGGGCAGCGGGTGCCCCAGCTCGATGATGATCTTCATGCGGGGCAGGAAGATCCATTGCAGCCACTGCTCAAAACTGAGGGTATC‑‑CACGGCGAACGGCACCGTACTGGCCAGTGCCTCGTCGCtt                                                                    >  6:139879/1‑149 (MQ=255)                                                                              gACAAGGATGCTCGATGCATTGGGCAGCGGGTGCCCCAGCTCGATGATGATCTTCATGCGGGGCAGGAAGATCCATTGCAGCCACTGCTCAAAACTGAGGGTATC‑‑CACGGCGAACGGCACCGTACTGGCCAGTGCCTCGTCGCTTggcg                                                                >  5:21823/1‑149 (MQ=255)                                                                              gACAAGGATGCTCGATGCATTGGGCAGCGGGTGCCCCAGCTCGATGATGATCTTCATGCGGGGCAGGAAGATCCATTGCAGCCACTGCTCAAAACTGAGGGTATC‑‑CACGGCGAACGGCACCGTACTGGCCAGTGCCTCGTCGCTTggcg                                                                >  2:289497/1‑149 (MQ=255)                                                                                cAAGGCTGCTAGATGCATTGGGCAGCGGGTGCCCCAGCTCGATGATGATCTTCATGCGGGGCAGGAAGATCCATTGCAGCCACTGCTCAAAACTGAGGGTATC‑‑CACGGCGAACGGCACCGTACTGGCCAGTGCCTCGTCGCTTggcggc                                                              <  8:293699/149‑1 (MQ=255)                                                                                    gATGCTCGATGCATTGGGCAGCGGGTGCCCCAGCTCGATGATGATCTTCATGCGGGGCAGGAAGATCCATTGCAGCCACTGCTCAAAACTGAGGGTATC‑‑CACGGCGAACGGCACCGTACTGGCCAGTGCCTCGTCGCTTGGCGGCTCGt                                                          <  5:354336/149‑1 (MQ=255)                                                                                     aTGCTCGATGCATTGGGCAGCGGGTGCCCCAGCTCGATGATGATCTTCATGCGGGGCAGGAAGATCCATTGCAGCCACTGCTCAAAACTGAGGGTATC‑‑CACGGCGAACGGCACCGTACTGGCCAGTGCCTCGTCGCTTGGCGGCTCGTc                                                         <  2:85554/149‑1 (MQ=255)                                                                                                aTTGGGCAGCGGGTGCCCCAGCTCGATGATGATCTTCATGCGGGGCAGGAAGATCCATTGCAGCCACTGCTCAAAACTGAGGGTATC‑‑CACGGCGAACGGCACCGTACTGGCCAGTGCCTCGTCGCTTGGCGGCTCGTCATCCCACCAAc                                              >  4:163470/1‑149 (MQ=255)                                                                                                 ttGGGCAGCGGGTGCCCCAGCTCGATGATGATCTTCATGCGGGGCAGGAAGATCCATTGCAGCCACTGCTCAAAACTGAGGGTATC‑‑CACGGCGAACGGCACCGTACTGGCCAGTGCCTCGTCGCTTGGCGGCTCGTCATCCCACCAAcc                                             >  6:73414/1‑149 (MQ=255)                                                                                                    ggCAGCGGGTGCCCCAGCTCGATGATGATCTTCATGCGGGGCAGGAAGATCCATTGCAGCCACTGCTCAAAACTGAGGGTATCCTCACGGCGAACGGCACCGTACTGGCCAGTGCCTCGTCGCTTGGCGGCTCGTCATCCCACCAACCt                                            <  1:289497/149‑1 (MQ=255)                                                                                                    ggCAGCGGGTGCCCCAGCTCGATGATGATCTTCATGCGGGGCAGGAAGATCCATTGCAGCCACTGCTCAAAACTGAGGGTATC‑‑CACGGCGAACGGCACCGTACTGGCCAGTGCCTCGTCGCTTGGCGGCTCGTCATCCCACCAACCtt                                           <  5:73414/148‑1 (MQ=255)                                                                                                     gCAGCGGGTGCCCCAGCTCGATGATGATCTTCATGCGGGGCAGGAAGATCCATTGCAGCCACTGCTCAAAACTGAGGGTATC‑‑CACGGCGAACGGCACCGTACTGGCCAGTGCCTCGTCGCTTGGCGGCTCGTCATCCCACCAACCTTg                                          >  4:295604/1‑148 (MQ=255)                                                                                                      cAGCGGGTGCCCCAGCTCGATGATGATCTTCATGCGGGGCAGGAAGATCCATTGCAGCCACTGCTCAAAACTGAGGGTATC‑‑CACGGCGAACGGCACCGTACTGGCCAGTGCCTCGTCGCTTGGCGGCTCGTCATCCCACCAACCTTGCa                                        <  5:139879/149‑1 (MQ=255)                                                                                                       aGCGGGTGCCCCAGCTCGATGATGATCTTCATGCGGGGCAGGAAGATCCATTGCAGCCACTGCTCAAAACTGAGGGTATC‑‑CACGGCGAACGGCACCGTACTGGCCAGTGCCTCGTCGCTTGGCGGCTCGTCATCCCACCAACCTTGCAc                                       <  3:295604/149‑1 (MQ=255)                                                                                                          gggTGCCCCAGCTCGATGATCATCTTCATGCGGGGCAGGAAGATCCATTGCCGCCAATGCTCAAAACTGAGGGTATC‑‑AACGGCGAACGGCACCGTACTGGCCAGTCCCTCGTCGCTTGGCGGCTCGTCATCCCACCAACCTTGAAcatt                                    >  3:94688/1‑146 (MQ=255)                                                                                                              gCCCCAGCTCGATGATGATCTTCATGCGGGGCAGGAAGATCCATTGCAGCCACTGCTCAAAACTGAGGGTATC‑‑CACGGCGAACGGCACCGTACTGGCCAGTGCCTCGTCGCTTGGCGGCTCGTCATCCCACCAACCtt                                           >  7:323927/1‑138 (MQ=255)                                                                                                              gCCCCAGCTCGATGATGATCTTCATGCGGGGCAGGAAGATCCATTGCAGCCACTGCTCAAAACTGAGGGTATC‑‑CACGGCGAACGGCACCGTACTGGCCAGTGCCTCGTCGCTTGGCGGCTCGTCATCCCACCAACCtt                                           <  8:323927/138‑1 (MQ=255)                                                                                                              gCCCCAGCTCGATGATGATCTTCATGCGGGGCAGGAAGATCCATTGCAGCCACTGCTCAAAACTGAGGGTATC‑‑CACGGCGAACGGCACCGTACTGGCCAGTGCCTCGTCGCTTGGCGGCTCGTCATCCCACCAACCTTGCACCTGctct                                >  1:286410/1‑146 (MQ=255)                                                                                                                 ccAGCTCGATGATGATCTTCATGCGGGGCAGGAAGATCCATTGCAGCCACTGCTCAAAACTGAGGGTATC‑‑CACGGCGAACGGCACCGTACTGGCCAGTGCCTCGTCGCTTGGCGGCTCGTCATCCCACCAACCTTGCACCTGCAGCTcg                             >  1:242672/1‑149 (MQ=255)                                                                                                                 ccAGCTCGATGATGATCTTCATGCGGGGCAGGAAGATCCATTGCAGCCACTGCTCAAAACTGAGGGTATC‑‑CACGGCGAACGGCACCGTACTGGCCAGTGCCTCGTCGCTTGGCGGCTCGTCATCCCACCAACCTTGCACCTGCAGCTcg                             >  7:217541/1‑149 (MQ=255)                                                                                                                  cAGCTCGATGATGATCTTCATGCGGGGCAGGAAGATCCATTGCAGCCACTGCTCAAAACTGAGGGTATC‑‑CACGGCGAACGGCACCGTACTGGCCAGTGc                                                                              <  5:286770/99‑1 (MQ=255)                                                                                                                  cAGCTCGATGATGATCTTCATGCGGGGCAGGAAGATCCATTGCAGCCACTGCTCAAAACTGAGGGTATC‑‑CACGGCGAACGGCACCGTACTGGCCAGTGc                                                                              >  6:286770/1‑99 (MQ=255)                                                                                                                    gCTCGATGATGATCTTCATGCGGGGCAGGAAGATCCATTGCAGCCACTGCTCAAAACTGAGGGTATC‑‑CACGGCGAACGGCACCGTACTGGTCAGTGCCTCGTCGCTTGGCGGCTCGTCATCCCACCAACCTTGCACCTGCAGCTCgcgc                          <  3:165680/149‑1 (MQ=255)                                                                                                                       cGATGATGATCTTCATGCGGGGCAGGAAGATCCATTGCAGCCACTGCTCAAAACTGAGGGTATC‑‑CACGGCGAACGGCACCGTACTGGCCAGTGCCTCGTCGCTTGGCGGCTCGTCATCCCACCAACCTTGCACCTGCAGCTCGCGCTc                        >  6:113368/1‑148 (MQ=255)                                                                                                                        gatgatgatCTTCATGCGGGGCAGGAAGATCCATTGCAGCCACTGCTCAAAACTGAGGGTATC‑‑CACGGCGAACGGCACCGTACTGGCCAGTGCCTCGTCGCTTGGCGGCTCGTCATcc                                                     >  5:99883/1‑118 (MQ=255)                                                                                                                        gatgatgatCTTCATGCGGGGCAGGAAGATCCATTGCAGCCACTGCTCAAAACTGAGGGTATC‑‑CACGGCGAACGGCACCGTACTGGCCAGTGCCTCGTCGCTTGGCGGCTCGTCATcc                                                     <  6:99883/118‑1 (MQ=255)                                                                                                                        gatgatgatCTTCATGCGGGGCAGGAAGATCCATTGCAGCCACTGCTCAAAACTGAGGGTATC‑‑CACGGCGAACGGCACCGTACTGGCCAGTGCCTCGTCGCTTGGCGGCTCGTCATCCCACCAACCTTGCACCTGCAGCTCGCGCTCGa                      >  3:174090/1‑149 (MQ=255)                                                                                                                           gatgatCTTCATGCGGGGCAGGAAGATCCATTGCAGCCACTGCTCAAAACTGAGGGTATC‑‑CACGGCGAACGGCACCGTACTGGCCAGTGCCTCGTCGCTTGGCGGCTCGTCATCCCACCAACCTTGCACCTGCAGCTCGCGCTCGATc                    >  2:128141/1‑148 (MQ=255)                                                                                                                           gatgatCTTCATGCGGGGCAGGAAGATCCATTGCAGCCACTGCTCAAAACTGAGGGTATC‑‑CACGGCGAACGGCACCGTACTGGCCAGTGCCTCGTCGCTTGGCGGCTCGTCATCCCACCAACCTTGCACCTGCAGCTCGCGCTCGATc                    <  6:21823/148‑1 (MQ=255)                                                                                                                            atgatCTTCATGCGGGGCAGGAAGATCCATTGCAGCCACTGCTCAAAACTGAGGGTATC‑‑CACGGCGAACGGCACCGTACTGGCCAGTGCCTCGTCGCTTGGCGGCTCGTCATCCCACCAACCTTGCACCTGCAGCTCGCGCTCGATCaa                  <  8:217541/149‑1 (MQ=255)                                                                                                                                  ttCATGCGGGGCAGGAAGATCCATTGCAGCCACTGCTCAAAACTGAGGGTATC‑‑CACGGCGAACGGCACCGTACTGGCCAGTGCCTCGTCGCTTGGCGGCTCGTCATCCCACCAACCTTGCACCTGCAGCTCGCGCTCGATCAAGAGCAg            <  4:296348/149‑1 (MQ=255)                                                                                                                                     aTGCGGGGCTGGAAGATCCATTGCAGCGACTGCTCAAATCTGAGGGTATC‑‑CACGGCGAACTGCACCGTACTGGCCAGTGCCTCGTCGCTTGGCGGCTCGTCATCCCACCAACCTTGCACCTGCTGCTCGCGCTCGATCAAGAGCAggt          <  3:9698/148‑1 (MQ=255)                                                                                                                                     aTGCGGGGCAGGAAGATCCATTGCAGCCACTGCTCAAAACTGAGGGTATC‑‑CACGGCGAACGGCACCGTACTGGCCAGTGCCTCGTCGCTTGGCGGCTCGTCATCCCACCAACCTTGCACCTGCAGCTCGCGCTc                        >  7:187623/1‑134 (MQ=255)                                                                                                                                     aTGCGGGGCAGGAAGATCCATTGCAGCCACTGCTCAAAACTGAGGGTATC‑‑CACGGCGAACGGCACCGTACTGGCCAGTGCCTCGTCGCTTGGCGGCTCGTCATCCCACCAACCTTGCACCTGCAGCTCGCGCTc                        <  8:187623/134‑1 (MQ=255)                                                                                                                                     aTGCGGGCCAGCAAGATCCATTGCAGCCACACCTCAAAACTGAGGGTATC‑‑CACGGCGAACGGCACCTTACTGGCCAGTGACTCGTCGCTAGCCGGCTCGACATCCACCCAACCTTGCACCTGCACATCGCGCTCGATCAAAAGCAg            >  4:9698/1‑146 (MQ=255)                                                                                                                                      tGCGGGGCAGGAAGATCCATTGCAGCCACTGCTCAAAACTGAGGGTATC‑‑CACGGCGAACGGCa                                                                                              >  3:248192/1‑63 (MQ=255)                                                                                                                                      tGCGGGGCAGGAAGATCCATTGCAGCCACTGCTCAAAACTGAGGGTATC‑‑CACGGCGAACGGCa                                                                                              <  4:248192/63‑1 (MQ=255)                                                                                                                                         ggggCAGGAAGATCCATTGCAGCCACTGCTCAAAACTGAGGGTATC‑‑CACGGCGAACGGCACCGTACTGGCCAGTGCCTCGTCGCTTggcgg                                                               <  2:53594/91‑1 (MQ=255)                                                                                                                                         ggggCAGGAAGATCCATTGCAGCCACTGCTCAAAACTGAGGGTATC‑‑CACGGCGAACGGCACCGTACTGGCCAGTGCCTCGTCGCTTggcgg                                                               >  1:53594/1‑91 (MQ=255)                                                                                                                                            gCAGGAAGATCCATTGCAGCCACTGCTCAAAACTGAGGGTATC‑‑CACGGCGAACGGCACCGTACTGGCCAGTGCCTCGTCGCTTGGCGGCTCGTCATCCCACCAACCTTGCACCTGCAGCTCGCGCTCGATCAAGAGCAGGTGGTCGGcc  <  3:163470/149‑2 (MQ=255)                                                                                                                                            gCAGGAAGATCCATTGCAGCCACTGCTCAAAACTGAGGGTATC‑‑CACGGCGAACGGCACCGTACTGGCCAGTGCCTCGTCGCTTGGCGGCTCGTCATCCCACCAACCTTGCACCTGCAGCTCGCGCTCGATCAAGAGCAGGTGGTCGGc   >  3:153388/1‑148 (MQ=255)                                                                                                                                              aGGAAGATCCATTGCAGCCACTGCTCAAAACTGAGGGTATC‑‑CACGGCGAACGGCACCGTACTGGCCAGTGCCTCGTCGCTTGGCGGCTCGTCATCCCACCAACCTTGCACCTGCAGCTCGCGCTCGATCAAGAGCAg            <  2:240174/137‑1 (MQ=255)                                                                                                                                              aGGAAGATCCATTGCAGCCACTGCTCAAAACTGAGGGTATC‑‑CACGGCGAACGGCACCGTACTGGCCAGTGCCTCGTCGCTTGGCGGCTCGTCATCCCACCAACCTTGCACCTGCAGCTCGCGCTCGATCAAGAGCAg            >  1:240174/1‑137 (MQ=255)                                                                                                                                               |                                                                                                                                                    AATTGATCAAAAGCTGCCAACAGGCGGCGTAGCTCACGACTTTGCTCCGGCCGGTCGGTAAACACGGTTTCGGCCATGACAAGGATGCTCGATGCATTGGGCAGCGGGTGCCCCAGCTCGATGATGATCTTCATGCGGGGCAGGAAGATCCATTGCAGCCACTGCTCAAAACTGAGGGTATC‑‑CACGGCGAACGGCACCGTACTGGCCAGTGCCTCGTCGCTTGGCGGCTCGTCATCCCACCAACCTTGCACCTGCAGCTCGCGCTCGATCAAGAGCAGGTGGTCGGCA  >  NC_002947/5319801‑5320088

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑