Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,324,157 T→G 7.7% L264R (CTG→CGG)  PP_4684 → kinase‑like domain‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,324,1570TG 7.7% 195.2 / 6.2 65L264R (CTG→CGG) PP_4684kinase‑like domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base T (24/36);  new base G (3/2);  total (27/38)
Fisher's exact test for biased strand distribution p-value = 6.42e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.82e-01

ACATCCACCTGGGCAACGCCACCTTGATCGATGGCAAGGTGGTGATCTTCGATTGCATCGAGTTCAACGAGCCTTTCCGACTGACCGACGTCTACGCCGACACCGCCTTCCTGGCCATGGACCTGGAAGACCGCGGCCTGAAGTGCCTGGCCCGGCGCTTCATCAGCCAGTACCTGGAACTGACGGGGGATTACGAAGGCCTGGAACTGCTCAACTTCTACAAAGCCTACCGCGCGCTCGTACGGGCCAAGGTCGCATTGTTCAGCATGCCGGCCGACGCTGATGGCGTTCAGC  >  NC_002947/5324010‑5324303
                                                                                                                                                   |                                                                                                                                                  
aCATCCACCTGGGCAACGCCACCTTGATCGATGGCAAGGTGGTGATCTTCGATTGCATCGAGTTCAACGAGCCTTTCCGACTGACCGACGTCTACGCCGACACCGCCTTCCTGGCCATGGACCTGGAAGACCGCGGCCTGAAGTGCCTg                                                                                                                                                   <  3:97488/149‑1 (MQ=255)
 cATCCACCTGGGCAACGCCAACTTGATCGATGGCAAGGTGGTGATCTTCGATTGCATCGAGTTCAACGAGCCTTTCCGACTGACCGACGTCTACGCCGACACCGCCTTCCTGGCCATGGACCTGGAAGACCGCGGCCTGAAGTGCCTg                                                                                                                                                   <  3:220512/148‑1 (MQ=255)
  aTCCACCTGGGCAACGCCACCTTGATCGATGGCAAGGTGGTGATCTTCGATTGCATCGAGTTCAACGAGCCTTTCCGACTGACCGACGTCTACGCCGACACCGCCTTCCTGGCCATGGACCTGGAAGACCGCGGCCTGAAGTGCCTGGc                                                                                                                                                 <  4:214296/149‑1 (MQ=255)
  aTCCACCTGGGCAACGCCACCTTGATCGATGGCAAGGTGGTGATCTTCGATTGCATCGAGTTCAACGAGCCTTTCCGACTGACCGACGTCTACGCCGACACCGCCTTCCTGGCCATGGACCTGGAAGACCGCGGCCTGAAGTGCCTGGc                                                                                                                                                 >  2:286867/1‑149 (MQ=255)
     cACCTGGGCAACGCCACCTTGATCGATGGCAAGGTGGTGATCTTCGATTGCATCGAGTTCAACGAGCCTTTCCGACTGACCGACGTCTACGCCGACACCGCCTTCCTGGCCATGGACCTGGAAGACCGCGGCCTGAAGTGCCTGGCCCg                                                                                                                                              >  2:80750/1‑149 (MQ=255)
      aCCTGGGCAACGCCACCTTGATCGATGGCAAGGTGGTGATCTTCGATTGCATCGAGTTCAACGAGCCTTTCCGACTGACCGACGTCTACGCCGACACCGCCTTCCTGGCCATGGACCTGGAAGACCGCGGCCTGAAGTGCCTGGCCCgg                                                                                                                                             <  7:31135/149‑1 (MQ=255)
      aCCTGGGCAACGCCACCTTGATCGATGGCAAGGTGGTGATCTTCGATTGCATCGAGTTCAACGAGCCTTTCCGACTGACCGACGTCTACGCCGACACCGCCTTCCTGGCCATGGACCTGGAAGACCGCGGCCTGAAGTGCCTGGCCCgg                                                                                                                                             >  5:280066/1‑149 (MQ=255)
       ccTGGGCAACGCCACCTTGATCGATGGCAAGGTGGTGATCTTCGATTGCATCGAGTTCAACGAGCCTTTCCGACTGACCGACGTCTACGCCGACACCGCCTTCCTGGCCATGGACCTGGAAGACCGCGGCCTGAAGTGCCTGGCCCGgc                                                                                                                                            >  1:194028/1‑149 (MQ=255)
       ccTGGGCAACGCCACCTTGATCGATGGCAAGGTGGTGATCTTCGATTGCATCGAGTTCAACGAGCCTTTCCGACTGACCGACGTCTACGCCGACACCGCCTTCCTGGCCATGGACCTGGAAGACCGCGGCCTGAAGTGCCTGGCCCGgc                                                                                                                                            <  6:280066/149‑1 (MQ=255)
        cTGGGCAACGCCACCTTGATCGATGGCAAGGTGGTGATCTTCGATTGCATCGAGTTCAACGAGCCTTTCCGACTGACCGACGTCTACGCCGACACCGCCTTCCTGGCCATGGACCTGGAAGACCGCGGCCTGAAGTGCCTGGCCCGgcg                                                                                                                                           <  1:286867/149‑1 (MQ=255)
        cTGGGCAACGCCACCTTGATCGATGGCAAGGTGGTGATCTTCGATTGCATCGAGTTCAACGAGCCTTTCCGACTGACCGACGTCTACGCCGACACCGCCTTCCTGGCCATGGACCTGGAAGACCGCGGCCTGAAGTGCCTGGCCCGgcg                                                                                                                                           <  2:93100/149‑1 (MQ=255)
             cAACGCCACCTTGATCGATGGCAAGGTGGTGATCTTCGATTGCATCGAGTTCAACGAGCCTTTCCGACTGACCGACGTCTACGCCGACACCGCCTTCCTGGCCATGGACCTGGAAGACCGCGGCCTGAAGCGCCGGGCCAGGCACTtca                                                                                                                                      <  4:204439/149‑1 (MQ=255)
               aCGCCACCTTGATCGATGGCAAGGTGGTGATCTTCGATTGCATCGAGTTCAACGAGCCTTTCCGACTGACCGACGTCTACGCCGACACCGCCTTCCTGGCCATGGACCTGGAAGACCGCGGCCTGAAGTGCCTGGCCCGGCGCTtcat                                                                                                                                     >  1:136789/1‑148 (MQ=255)
                cGCCAACTTGATCGATGGCAATGTGGTGATCTTCTATTGCATCGAGTTCAACGAGCATTTCCGACTGACCGACGTTTACGCCGACACCGCCTTCCTTGCCATGGACCTGGAAGACCGCGGCATGAAGTGCCTGGCCCGGCGCTtcatca                                                                                                                                   <  2:60222/149‑1 (MQ=255)
                   cACCTTGATCGATGGCAAGGTGGTGATCTTCGATTGCATCGAGTTCAACGAGCCTTTCCGACTGACCGACGTCTACGCCGACACCGCCTTCCTGGCCATGGACCTGGAAGACCGCGGCCTGAAGTGCCTGGCCCGGCGCTTCATCAGc                                                                                                                                 >  2:220116/1‑148 (MQ=255)
                    aCCTTGATCGATGGCAAGGTGGTGATCTTCGATTGCATCGAGTTCAACGAGCCTTTCCGACTGACCGACGTCTACGCCGACACCGCCTTCCTGGCCATGGACCTGGAAGACCGCGGCCTGAAGTGCCTGGCCCGGCGCTTCATCAGCCa                                                                                                                               <  2:315562/149‑1 (MQ=255)
                    aCCTTGATCGATGGCAAGGTGGTGATCTTCGATTGCATCGAGTTCAACGAGCCTTTCCGACTGACCGACGTCTACGCCGACACCGCCTTCCTGGCCATGGACCTGGAAGACCGCGGCCTGAAGCGCCGGGCCAGGCACTtca                                                                                                                                      <  6:306589/142‑1 (MQ=255)
                    aCCTTGATCGATGGCAAGGTGGTGATCTTCGATTGCATCGAGTTCAACGAGCCTTTCCGACTGACCGACGTCTACGCCGACACCGCCTTCCTGGCCATGGACCTGGAAGACCGCGGCCTGAAGCGCCGGGCCAGGCACTtca                                                                                                                                      >  5:306589/1‑142 (MQ=255)
                         gATCGATGGCAAGGTGGTGATCTTCGATTGCATCGAGTTCAACGAGCCTTTCCGACTGACCGACGTCTACGCCGACACCGCCTTCCTGGCCATGGACCTGGAAGACCGCGGCCTGAAGTGCCTGGCCCGGCGCTTCATCCGCCAGTa                                                                                                                            <  7:174540/147‑1 (MQ=255)
                         gATCGATGGCAAGGTGGTGATCTTCGATTGCATCGAGTTCAACGAGCCTTTCCGACTGACCGACGTCTACGCCGACACCGCCTTCCTGGCCATGGACCTGGAAGACCGCGGCCTGAAGTGCCTGGCCCGGCGCTTCATCAGCCAGTAcc                                                                                                                          >  4:278806/1‑149 (MQ=255)
                           tCGATGGCAAGGTGGTGATCTTCGATTGCATCGAGTTCAACGAGCCTTTCCGACTGACCGACGTCTACGCCGACACCGCCTTCCTGGCCATGGACCTGGAAGACCGCGGCCTGAAGTGCCTGGCCCGGCGCTTCATCAGCCAGTACCTg                                                                                                                        <  2:194028/149‑1 (MQ=255)
                              aTGGCAAGGTGGTGATCTTCGATTGCATCGAGTTCAACGAGCCTTTCCGACTGACCGACGTCTACGCCGACACCGCCTTCCTGGCCATGGACCTGGAAGACCGCGGCCTGAAGTGCCTGGCCCGGCGCTTCATCAGCCAGTACCTGGaa                                                                                                                     <  6:65301/149‑1 (MQ=255)
                                  cAAGGTGGTGATCTTCGATTGCATCGAGTTCAACGAGCCTTTCCGACTGACCGACGTCTACGCCGACACCGCCTTCCTGGCCATGGACCTGGAAGACCGCGGCCTGAAGTGCCTGGCCCGGCGCTTCATCAGCCAGTACCTGGAACTGa                                                                                                                 >  3:119816/1‑149 (MQ=255)
                                  cAAGGTGGTGATCTTCGATTGCATCGAGTTCAACGAGCCTTTCCGACTGACCGACGTCTACGCCGACACCGCCTTCCTGGCCATGGACCTGGAAGACCGCGGCCTGAAGTGCCTGGCCCGGCGCTTCATCAGCCAGTACCTGGAACTGa                                                                                                                 <  2:136789/149‑1 (MQ=255)
                                       tggtgATCTTCGATTGCATCGAGTTCAACGAGCCTTTCCGACTGACCGACGTCTACGCCGACACCGCCTTCCTGGCCATGGACCTGGAAGACCGCGGCCTGAAGTGCCTGGCCCGGCGCTTCATCAGCCAGTACCTGGAACTGACgggg                                                                                                            <  4:319216/149‑1 (MQ=255)
                                          tgATCTTCGATTGCATCGAGTTCAACGAGCCTTTCCGACTGACCGACGTCTACGCCGACACCGCCTTCCTGGCCATGGACCTGGAAGACCGCGGCCTGAAGTGCCTGGCCCGGCGCTTCATCAGCCAGTACCTGGAACTGACGGGGGAt                                                                                                         >  6:366260/1‑149 (MQ=255)
                                             tCTTCGATTGCATCGAGTTCAACTAGCCTTTCCGACTGACCGACGTCTACGCCGACACCGCCTTCCTTGCCATGGACCTTGAAGACCGCGGCCTGAAGTGCCTGGCCCGGCGCTTCATCAGCCAGTACCTGGAACTGACGGGGGATTa                                                                                                       <  8:89018/148‑1 (MQ=255)
                                                  gATTGCATCGAGTTCAACGAGCCTTTCCGACTGACCGACGTCTACGCCGACACCGCCTTCCTGGCCATGGACCTGGAAGACCGCGGCCTGAAGTGCCTGGCCCGGCGCTTCATCAGCCAGTACCTGGAACTGACGGGGGATTACGAAgg                                                                                                 >  5:68045/1‑149 (MQ=255)
                                                  gATTGCATCGAGTTCAACGAGCCTTTCCGACTGACCGACGTCTACGCCGACACCGCCTTCCTGGCCATGGACCTGGAAGACCGCGGCCTGAAGTGCCTGGCCCGGCGCTTCATCAGCCAGTACCTGGAACTGACGGGGGATTACGAAg                                                                                                  <  3:138662/148‑1 (MQ=255)
                                                    ttGCATCGAGTTCAACGAGCCTTTCCGACTGACCGACGTCTACGCCGACACCGCCTTCCTGGCCATGGACCTGGAAGACCGCGGCCTGAAGTGCCTGGCCCGGCGCTTCATCAGCCAGTACCTGGAACTGACGGGGGATTACGAAGGcc                                                                                               <  5:366260/149‑1 (MQ=255)
                                                       cATCGAGTTCAACGAGCCTTTCCGACTGACCGACGTCTACGCCGACACCGCCTTCCTGGCCATGGACCTGGAAGACCGCGGCCTGAAGTGCCTGGCCCGGCGCTTCATCAGCCAGTACCTGGAACTGACGGGGGATTACGAAGGCCTgg                                                                                            <  8:20859/149‑1 (MQ=255)
                                                        aTCGAGTTCAACGAGCCTTTCCGACTGACCGACGTCTACGCCGACACCGCCTTCCTGGCCATGGACCTGGAAGACCGCGGCCTGAAGTGCCTGGCCCGGCGCTTCATCAGCCAGTACCTGGAACTGACGGGGGATTACGAAGGCCTgg                                                                                            >  4:305956/1‑148 (MQ=255)
                                                         tCGAGTTCAACGAGCCTTTCCGACTGACCGACGTCTACGCCGACACCGCCTTCCTGGCCATGGACCTGGAAGACCGCGGCCTGAAGTGCCTGGCCCGGCGCTTCATCAGCCAGTACCTGGAACTGACGGGGGATTACGAAGGCCTg                                                                                             <  7:167705/146‑1 (MQ=255)
                                                         tCGAGTTCAACGAGCCTTTCCGACTGACCGACGTCTACGCCGACACCGCCTTCCTGGCCATGGACCTGGAAGACCGCGGCCTGAAGTGCCTGGCCCGGCGCTTCATCAGCCAGTACCTGGAACTGACGGGGGATTACGAAGGCCTg                                                                                             >  8:167705/1‑146 (MQ=255)
                                                         tCGAGTTCAACGAGCCTTTCCGACTGACCGACGTCTACGCCGACACCGCCTTCCTGGCCATGGACCTGGAAGACCGCGGCCTGAAGTGCCTGGCCCGGCGCTTCATCAGCCAGTACCTGGAACTGACGGGGGATTACGAAGGCCTGGaa                                                                                          <  3:305956/149‑1 (MQ=255)
                                                         tCGAGTTCAACGAGCCTTTCCGACTGACCGACGTCTACGCCGACACCGCCTTCCTGGCCATGGACCTGGAAGACCGCGGCCTGAAGTGCCTGGCCCGGCGCTTCATCAGCCAGTACCTGGAACTGACGGGGGATTACGAAGGCCTGGaa                                                                                          >  6:71049/1‑149 (MQ=255)
                                                              ttCAACGAGCCTTTCCGACTGACCGACGTCTACGCCGACACCGCCTTCCTGGCCATGGACCTGGAAGACCGCGGCCTGAAGTGCCTGGCCCGGCGCTTCATCAGCCAGTACCTGGAACTGACGGGGGATTACGAAGGCCTGGAACTGCt                                                                                     <  4:119816/149‑1 (MQ=255)
                                                                cAACTAGCCTTTCCCACTCCCCCAGGTCTACGCCTACACCGCTTTCGTGCCAATGAACCTGTAATACCGCGGCCGTAATTGCCTGGCCCGGCGCTTCATCAGCCAGTACCTGGAACTGACGGGGGATTACGAAGGCCTGGAACTGCTc                                                                                    <  1:94284/148‑1 (MQ=255)
                                                                 aaCGAGCCTTTCCGACTGACCGACGTCTACGCCGACACCGCCTTCCTGGCCATGGACCTGGAAGACCGCGGCCTGAAGTGCCTGGCCCGGCGCTTCATCAGCCAGTACCTGGAACTGACGGGGGATTACGAAGGCCTGGAACTGCTCaa                                                                                  <  1:80750/149‑1 (MQ=255)
                                                                 aaCGAGCCTTTCCGACTGACCGACGTCTACGCCGACACCGCCTTCCT‑GCCATGGACCTGGAAGACCGCGGCCTGAAGTGCCTGGCCCGGCGCTTCATCAGCCAGTACCTGGAACTGACGGGGGATTACGAAGGCCTGGAACTGCTCaa                                                                                  <  1:237657/148‑1 (MQ=255)
                                                                  aCGAGCCTTTCCGACTGACCGACGTCTACGCCGACACCGCCTTCCTGGCCATGGACCTGGAAGACCGCGGCCTGAAGTGCCTGGCCCGGCGCTTCATCAGCCAGTACCTGGAACTGACGGGGGATTACGAAGGCCTGGAACTGCTCAAc                                                                                 <  1:220116/149‑1 (MQ=255)
                                                                  aCGAGCCTTTCCGACTGACCGACGTCTACGCCGACACCGCCTTCCTGGCCATGGACCTGGAAGACCGCGGCCTGAAGTGCCTGGCCCGGCGCTTCATCAGCCAGTACCTGGAACTGACGGGGGATTACGAAGGCCTGGAACTGCTCAAc                                                                                 <  7:2480/149‑1 (MQ=255)
                                                                     aGCCTTTCCGACTGACCGACGGCTACGCCGACACCGCCTTCCTGGCCATGGACCTGGAAGACCGCGGCCTGAAGTGCCTGGCCCGGCGCTTCATCAGCCAGTACCTGGAACTGACGGGGGATTACGAAGGCCTGGAACTGCTCAACTTc                                                                              <  6:68045/149‑1 (MQ=255)
                                                                         tttCCGACTGACCGACGTCTACGCCGACACCGCCTTCCTGGCCATGGACCTGGAAGACCGCGGCCTGAAGTGCCTGGCCCGGCGCTTCATCAGCCAGTACCTGGAACTGACGGGGGATTACGAAGGCCTTGAACTGCt                                                                                     <  2:1709/138‑1 (MQ=255)
                                                                         tttCCGACTGACCGACGTCTACGCCGACACCGCCTTCCTGGCCATGGACCTGGAAGACCGCGGCCTGAAGTGCCTGGCCCGGCGCTTCATCAGCCAGTACCTGGAACTGACGGGGGATTACGAAGGCCTGGAACTGCt                                                                                     >  1:1709/1‑138 (MQ=255)
                                                                            ccGACTGACCGACGTCTACGCCGACACCGCCTTCCTGGCCATGGACCTGGAAGACCGCGGCCTGAAGTGCCTGGCCCGGCGCTTCATCAGCCAGTACCTGGAACTGACGGGGGATTACGAAGGCCTGGAACTGCTCAACTTCTACaaa                                                                        <  3:351537/148‑1 (MQ=255)
                                                                                   aCCGACGTCTACGCCGACACCGCCTTCCTGGCCATGGACCTGGAAGACCGCGGCCTGAAGTGCCTGGCCCGGCGCTTCATCAGCCAGTACCTGGAACTGACGGGGGATTACGAAGGCCTGGAACTGCTCAACTTCTACAAAGCCTAcc                                                                 <  3:278806/148‑1 (MQ=255)
                                                                                                       cGCCTTCCTGGCCATGGACCTGGAAGACCGCGGCCTGAAGTGCCTGGCCCGGCGCTTCATCAGCCAGTACCTGGAACTGACGGGGGATTACGAAGGCCTGGAACTGCTCAACTTCTACAAAGCCTACCGCGCGCTCGTACGGGCCAAgg                                            >  5:303470/1‑149 (MQ=255)
                                                                                                        gCCTTCCTGGCCATGGACCTTGAAGACCGCGGCCTGAAGTGCCTGGCCCGGCGCTTCATCAGCCAGTACCTGGAACTGACGGGGGATTACGaa                                                                                                   <  4:181612/93‑1 (MQ=255)
                                                                                                        gCCTTCCTGGCCATGGACCTGGAAGACCGCGGCCTGAAGTGCCTGGCCCGGCGCTTCATCAGCCAGTACCTGGAACTGACGGGGGATTACGaa                                                                                                   >  3:181612/1‑93 (MQ=255)
                                                                                                                   cATGGACCTGGAAGACCGCGGCCTGAAGTGCCTGGCCCGGCGCTTCATCTGCCAGTACCTGGAACTGACGGGGGATGACGAAGGCCTGGAACTGCTCAAGTTCTACAAAGCCTACCGCGCGCGCGTACTGGCCAAGGTCGCAtt                                     <  7:140276/144‑1 (MQ=255)
                                                                                                                   cATGGACCTGGAAGACCGCGGCCTGAAGTGCCTGGCCCGGCGCTTCATCAGCCAGTACCTGGAACTGACGGGGGATTACGAAGGCCTGGAACTGCTCAACTTCTACAAAGACTACCGCGCGCTCGTACGGGCCAAGGTCCCAtt                                     >  8:140276/1‑144 (MQ=255)
                                                                                                                      ggACCTGGAAGACCGCGGCCTGAAGTGCCTGGCCCGGCGCTTCATCAGCCAGTACCTGGAACTGACGGGGGATTACGAAGGCCTGGAACTGCTCAACTTCTACAAAGCCTACCGCGCGCTCGTACGGGCCAAGGTCGCAt                                      <  1:175221/140‑1 (MQ=255)
                                                                                                                      ggACCTGGAAGACCGCGGCCTGAAGTGCCTGGCCCGGCGCTTCATCAGCCAGTACCTGGAACTGACGGGGGATTACGAAGGCCTGGAACTGCTCAACTTCTACAAAGCCTACCGCGCGCTCGTACGGGCCAAGGTCGCAt                                      >  2:175221/1‑140 (MQ=255)
                                                                                                                      ggACCTGGAAGACCGCGGCCTGAAGTGCCTGGCCCGGCGCTTCATCAGCCAGTACCTGGAACTGACGGGGGATTACGAAGGCCTGGAACTGCTCAACTTCTACAAAGCCTACCGCGCGCTCGTACGGGCCAAGGTCGCAt                                      <  3:77883/140‑1 (MQ=255)
                                                                                                                      ggACCTGGAAGACCGCGGCCTGAAGTGCCTGGCCCGGCGCTTCATCAGCCAGTACCTGGAACTGACGGGGGATTACGAAGGCCTGGAACTGCTCAACTTCTACAAAGCCTACCGCGCGCTCGTACGGGCCAAGGTCGCAt                                      >  4:77883/1‑140 (MQ=255)
                                                                                                                           tGGAAGACCGCGGCCTGAAGTGCCTGGCCCGGCGCTTCATCAGCCAGTACCTGGAACTGACGGGGGATTACGAAGGCCTGGAACTGCTCAACTTCTACAAAGCCTACCGCGCGCTCGTACGGGCCAAGGTCGCATTGTTc                                 >  2:23167/1‑140 (MQ=255)
                                                                                                                           tGGAAGACCGCGGCCTGAAGTGCCTGGCCCGGCGCTTCATCAGCCAGTACCTGGAACTGACGGGGGATTACGAAGGCCTGGAACTGCTCAACTTCTACAAAGCCTACCGCGCGCTCGTACGGGCCAAGGTCGCATTGTTc                                 <  1:23167/140‑1 (MQ=255)
                                                                                                                            ggAAGACCGCGGCCTGAAGTGCCTGGCCCGGCGCTTCATCAGCCAGTACCTGGAACTGACGGGGGATTACGAAGGCCTGGAACTGCTCAACTTCTACAAAGCCTACCGCGCGCTCGTACGGGCCAAGGTCGCATTGTTCAGCATgccgg                       >  5:125235/1‑149 (MQ=255)
                                                                                                                                   cgcgGCCTGAAGTGCCTGGCCCGGCGCTTCATCAGCCAGTACCTGGAACTGACGGGGGATTACGAAGGCCTGGAACTGCTCAACTTCTACAAAGCCTACCGCGCGCTCGTACGGGCCAAGGTCGCATTGt                                   >  5:282108/1‑130 (MQ=255)
                                                                                                                                   cgcgGCCTGAAGTGCCTGGCCCGGCGCTTCATCAGCCAGTACCTGGAACTGACGGGGGATTACGAAGGCCTGGAACTGCTCAACTTCTACAAAGCCTACCGCGCGCTCGTACGGGCCAAGGTCGCATTGt                                   <  6:282108/130‑1 (MQ=255)
                                                                                                                                      ctgatGAAGCGCCGGGCCAGGCACTTCATCAGCCAGTACCTGGAACTGACGGGGGATTACGAAGGCCTGGAACTGCTCAACTTCTACAAAGCCTACCGCGCGCTCGTACGGGCCAAGGTCGCATTGTTCAGCATGCCGGCCGACGCTGa             >  2:18580/5‑149 (MQ=255)
                                                                                                                                         atGAAGCGCCGGGCCAGGCACTTCATCAGCCAGTACCTGGAACTGACGGGGGATTACGAAGGCCTGGAACTGCTCAACTTCTACAAAGCCTACCGCGCGCTCGTACGGGCCAAGGTCGCATTGTTCAGCATGCCGGCCGACGCTGATgg          >  7:27611/2‑149 (MQ=255)
                                                                                                                                            aaGTGCCTGGCCCGGCGCTTCATCAGCCAGTACCTGGAACTGACGGGGGATTACGAAGGCCTGGAACTGCTCAACTTCTACAAAGCCTACCGCGCGCTCGTACGGGCCAAGGTCGCATTGTTCAGCATGCCGGCCGACGCTGATGGCGt       >  1:202048/1‑149 (MQ=255)
                                                                                                                                                 ccTGGCCCGGCGCTTCATCAGCCAGTACCTGGAACTGACGGGGGATTACGAAGGCCTGGAACTGCTCAACTTCTACAAAGCCTACCGCGCGCTCGTACGGGCCAAGGTCGCATTGTTCAGCATGCCGGCCGACGCTGATGGCGTTCAGc  >  8:152265/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
ACATCCACCTGGGCAACGCCACCTTGATCGATGGCAAGGTGGTGATCTTCGATTGCATCGAGTTCAACGAGCCTTTCCGACTGACCGACGTCTACGCCGACACCGCCTTCCTGGCCATGGACCTGGAAGACCGCGGCCTGAAGTGCCTGGCCCGGCGCTTCATCAGCCAGTACCTGGAACTGACGGGGGATTACGAAGGCCTGGAACTGCTCAACTTCTACAAAGCCTACCGCGCGCTCGTACGGGCCAAGGTCGCATTGTTCAGCATGCCGGCCGACGCTGATGGCGTTCAGC  >  NC_002947/5324010‑5324303

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑