Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,324,162 C→A 7.5% R266R (CGG→AGG)  PP_4684 → kinase‑like domain‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,324,1620CA 7.5% 190.3 / 4.3 62R266R (CGG→AGG) PP_4684kinase‑like domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base C (23/34);  new base A (3/2);  total (26/36)
Fisher's exact test for biased strand distribution p-value = 6.41e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.47e-01

CACCTGGGCAACGCCACCTTGATCGATGGCAAGGTGGTGATCTTCGATTGCATCGAGTTCAACGAGCCTTTCCGACTGACCGACGTCTACGCCGACACCGCCTTCCTGGCCATGGACCTGGAAGACCGCGGCCTGAAGTGCCTGGCCCGGCGCTTCATCAGCCAGTACCTGGAACTGACGGGGGATTACGAAGGCCTGGAACTGCTCAACTTCTACAAAGCCTACCGCGCGCTCGTACGGGCCAAGGTCGCATTGTTCAGCATGCCGGCCGACGCTGATGGCGTTCAGCGT  >  NC_002947/5324015‑5324305
                                                                                                                                                   |                                                                                                                                               
cACCTGGGCAACGCCACCTTGATCGATGGCAAGGTGGTGATCTTCGATTGCATCGAGTTCAACGAGCCTTTCCGACTGACCGACGTCTACGCCGACACCGCCTTCCTGGCCATGGACCTGGAAGACCGCGGCCTGAAGTGCCTGGCCCg                                                                                                                                                >  2:80750/1‑149 (MQ=255)
 aCCTGGGCAACGCCACCTTGATCGATGGCAAGGTGGTGATCTTCGATTGCATCGAGTTCAACGAGCCTTTCCGACTGACCGACGTCTACGCCGACACCGCCTTCCTGGCCATGGACCTGGAAGACCGCGGCCTGAAGTGCCTGGCCCgg                                                                                                                                               <  7:31135/149‑1 (MQ=255)
 aCCTGGGCAACGCCACCTTGATCGATGGCAAGGTGGTGATCTTCGATTGCATCGAGTTCAACGAGCCTTTCCGACTGACCGACGTCTACGCCGACACCGCCTTCCTGGCCATGGACCTGGAAGACCGCGGCCTGAAGTGCCTGGCCCgg                                                                                                                                               >  5:280066/1‑149 (MQ=255)
  ccTGGGCAACGCCACCTTGATCGATGGCAAGGTGGTGATCTTCGATTGCATCGAGTTCAACGAGCCTTTCCGACTGACCGACGTCTACGCCGACACCGCCTTCCTGGCCATGGACCTGGAAGACCGCGGCCTGAAGTGCCTGGCCCGgc                                                                                                                                              >  1:194028/1‑149 (MQ=255)
  ccTGGGCAACGCCACCTTGATCGATGGCAAGGTGGTGATCTTCGATTGCATCGAGTTCAACGAGCCTTTCCGACTGACCGACGTCTACGCCGACACCGCCTTCCTGGCCATGGACCTGGAAGACCGCGGCCTGAAGTGCCTGGCCCGgc                                                                                                                                              <  6:280066/149‑1 (MQ=255)
   cTGGGCAACGCCACCTTGATCGATGGCAAGGTGGTGATCTTCGATTGCATCGAGTTCAACGAGCCTTTCCGACTGACCGACGTCTACGCCGACACCGCCTTCCTGGCCATGGACCTGGAAGACCGCGGCCTGAAGTGCCTGGCCCGgcg                                                                                                                                             <  2:93100/149‑1 (MQ=255)
   cTGGGCAACGCCACCTTGATCGATGGCAAGGTGGTGATCTTCGATTGCATCGAGTTCAACGAGCCTTTCCGACTGACCGACGTCTACGCCGACACCGCCTTCCTGGCCATGGACCTGGAAGACCGCGGCCTGAAGTGCCTGGCCCGgcg                                                                                                                                             <  1:286867/149‑1 (MQ=255)
        cAACGCCACCTTGATCGATGGCAAGGTGGTGATCTTCGATTGCATCGAGTTCAACGAGCCTTTCCGACTGACCGACGTCTACGCCGACACCGCCTTCCTGGCCATGGACCTGGAAGACCGCGGCCTGAAGCGCCGGGCCAGGCACTtca                                                                                                                                        <  4:204439/149‑1 (MQ=255)
          aCGCCACCTTGATCGATGGCAAGGTGGTGATCTTCGATTGCATCGAGTTCAACGAGCCTTTCCGACTGACCGACGTCTACGCCGACACCGCCTTCCTGGCCATGGACCTGGAAGACCGCGGCCTGAAGTGCCTGGCCCGGCGCTtcat                                                                                                                                       >  1:136789/1‑148 (MQ=255)
           cGCCAACTTGATCGATGGCAATGTGGTGATCTTCTATTGCATCGAGTTCAACGAGCATTTCCGACTGACCGACGTTTACGCCGACACCGCCTTCCTTGCCATGGACCTGGAAGACCGCGGCATGAAGTGCCTGGCCCGGCGCTtcatca                                                                                                                                     <  2:60222/149‑1 (MQ=255)
              cACCTTGATCGATGGCAAGGTGGTGATCTTCGATTGCATCGAGTTCAACGAGCCTTTCCGACTGACCGACGTCTACGCCGACACCGCCTTCCTGGCCATGGACCTGGAAGACCGCGGCCTGAAGTGCCTGGCCCGGCGCTTCATCAGc                                                                                                                                   >  2:220116/1‑148 (MQ=255)
               aCCTTGATCGATGGCAAGGTGGTGATCTTCGATTGCATCGAGTTCAACGAGCCTTTCCGACTGACCGACGTCTACGCCGACACCGCCTTCCTGGCCATGGACCTGGAAGACCGCGGCCTGAAGTGCCTGGCCCGGCGCTTCATCAGCCa                                                                                                                                 <  2:315562/149‑1 (MQ=255)
               aCCTTGATCGATGGCAAGGTGGTGATCTTCGATTGCATCGAGTTCAACGAGCCTTTCCGACTGACCGACGTCTACGCCGACACCGCCTTCCTGGCCATGGACCTGGAAGACCGCGGCCTGAAGCGCCGGGCCAGGCACTtca                                                                                                                                        <  6:306589/142‑1 (MQ=255)
               aCCTTGATCGATGGCAAGGTGGTGATCTTCGATTGCATCGAGTTCAACGAGCCTTTCCGACTGACCGACGTCTACGCCGACACCGCCTTCCTGGCCATGGACCTGGAAGACCGCGGCCTGAAGCGCCGGGCCAGGCACTtca                                                                                                                                        >  5:306589/1‑142 (MQ=255)
                    gATCGATGGCAAGGTGGTGATCTTCGATTGCATCGAGTTCAACGAGCCTTTCCGACTGACCGACGTCTACGCCGACACCGCCTTCCTGGCCATGGACCTGGAAGACCGCGGCCTGAAGTGCCTGGCCCGGCGCTTCATCCGCCAGTa                                                                                                                              <  7:174540/147‑1 (MQ=255)
                    gATCGATGGCAAGGTGGTGATCTTCGATTGCATCGAGTTCAACGAGCCTTTCCGACTGACCGACGTCTACGCCGACACCGCCTTCCTGGCCATGGACCTGGAAGACCGCGGCCTGAAGTGCCTGGCCCGGCGCTTCATCAGCCAGTAcc                                                                                                                            >  4:278806/1‑149 (MQ=255)
                      tCGATGGCAAGGTGGTGATCTTCGATTGCATCGAGTTCAACGAGCCTTTCCGACTGACCGACGTCTACGCCGACACCGCCTTCCTGGCCATGGACCTGGAAGACCGCGGCCTGAAGTGCCTGGCCCGGCGCTTCATCAGCCAGTACCTg                                                                                                                          <  2:194028/149‑1 (MQ=255)
                         aTGGCAAGGTGGTGATCTTCGATTGCATCGAGTTCAACGAGCCTTTCCGACTGACCGACGTCTACGCCGACACCGCCTTCCTGGCCATGGACCTGGAAGACCGCGGCCTGAAGTGCCTGGCCCGGCGCTTCATCAGCCAGTACCTGGaa                                                                                                                       <  6:65301/149‑1 (MQ=255)
                             cAAGGTGGTGATCTTCGATTGCATCGAGTTCAACGAGCCTTTCCGACTGACCGACGTCTACGCCGACACCGCCTTCCTGGCCATGGACCTGGAAGACCGCGGCCTGAAGTGCCTGGCCCGGCGCTTCATCAGCCAGTACCTGGAACTGa                                                                                                                   >  3:119816/1‑149 (MQ=255)
                             cAAGGTGGTGATCTTCGATTGCATCGAGTTCAACGAGCCTTTCCGACTGACCGACGTCTACGCCGACACCGCCTTCCTGGCCATGGACCTGGAAGACCGCGGCCTGAAGTGCCTGGCCCGGCGCTTCATCAGCCAGTACCTGGAACTGa                                                                                                                   <  2:136789/149‑1 (MQ=255)
                                  tggtgATCTTCGATTGCATCGAGTTCAACGAGCCTTTCCGACTGACCGACGTCTACGCCGACACCGCCTTCCTGGCCATGGACCTGGAAGACCGCGGCCTGAAGTGCCTGGCCCGGCGCTTCATCAGCCAGTACCTGGAACTGACgggg                                                                                                              <  4:319216/149‑1 (MQ=255)
                                     tgATCTTCGATTGCATCGAGTTCAACGAGCCTTTCCGACTGACCGACGTCTACGCCGACACCGCCTTCCTGGCCATGGACCTGGAAGACCGCGGCCTGAAGTGCCTGGCCCGGCGCTTCATCAGCCAGTACCTGGAACTGACGGGGGAt                                                                                                           >  6:366260/1‑149 (MQ=255)
                                        tCTTCGATTGCATCGAGTTCAACTAGCCTTTCCGACTGACCGACGTCTACGCCGACACCGCCTTCCTTGCCATGGACCTTGAAGACCGCGGCCTGAAGTGCCTGGCCCGGCGCTTCATCAGCCAGTACCTGGAACTGACGGGGGATTa                                                                                                         <  8:89018/148‑1 (MQ=255)
                                             gATTGCATCGAGTTCAACGAGCCTTTCCGACTGACCGACGTCTACGCCGACACCGCCTTCCTGGCCATGGACCTGGAAGACCGCGGCCTGAAGTGCCTGGCCCGGCGCTTCATCAGCCAGTACCTGGAACTGACGGGGGATTACGAAgg                                                                                                   >  5:68045/1‑149 (MQ=255)
                                             gATTGCATCGAGTTCAACGAGCCTTTCCGACTGACCGACGTCTACGCCGACACCGCCTTCCTGGCCATGGACCTGGAAGACCGCGGCCTGAAGTGCCTGGCCCGGCGCTTCATCAGCCAGTACCTGGAACTGACGGGGGATTACGAAg                                                                                                    <  3:138662/148‑1 (MQ=255)
                                               ttGCATCGAGTTCAACGAGCCTTTCCGACTGACCGACGTCTACGCCGACACCGCCTTCCTGGCCATGGACCTGGAAGACCGCGGCCTGAAGTGCCTGGCCCGGCGCTTCATCAGCCAGTACCTGGAACTGACGGGGGATTACGAAGGcc                                                                                                 <  5:366260/149‑1 (MQ=255)
                                                  cATCGAGTTCAACGAGCCTTTCCGACTGACCGACGTCTACGCCGACACCGCCTTCCTGGCCATGGACCTGGAAGACCGCGGCCTGAAGTGCCTGGCCCGGCGCTTCATCAGCCAGTACCTGGAACTGACGGGGGATTACGAAGGCCTgg                                                                                              <  8:20859/149‑1 (MQ=255)
                                                   aTCGAGTTCAACGAGCCTTTCCGACTGACCGACGTCTACGCCGACACCGCCTTCCTGGCCATGGACCTGGAAGACCGCGGCCTGAAGTGCCTGGCCCGGCGCTTCATCAGCCAGTACCTGGAACTGACGGGGGATTACGAAGGCCTgg                                                                                              >  4:305956/1‑148 (MQ=255)
                                                    tCGAGTTCAACGAGCCTTTCCGACTGACCGACGTCTACGCCGACACCGCCTTCCTGGCCATGGACCTGGAAGACCGCGGCCTGAAGTGCCTGGCCCGGCGCTTCATCAGCCAGTACCTGGAACTGACGGGGGATTACGAAGGCCTg                                                                                               >  8:167705/1‑146 (MQ=255)
                                                    tCGAGTTCAACGAGCCTTTCCGACTGACCGACGTCTACGCCGACACCGCCTTCCTGGCCATGGACCTGGAAGACCGCGGCCTGAAGTGCCTGGCCCGGCGCTTCATCAGCCAGTACCTGGAACTGACGGGGGATTACGAAGGCCTg                                                                                               <  7:167705/146‑1 (MQ=255)
                                                    tCGAGTTCAACGAGCCTTTCCGACTGACCGACGTCTACGCCGACACCGCCTTCCTGGCCATGGACCTGGAAGACCGCGGCCTGAAGTGCCTGGCCCGGCGCTTCATCAGCCAGTACCTGGAACTGACGGGGGATTACGAAGGCCTGGaa                                                                                            <  3:305956/149‑1 (MQ=255)
                                                    tCGAGTTCAACGAGCCTTTCCGACTGACCGACGTCTACGCCGACACCGCCTTCCTGGCCATGGACCTGGAAGACCGCGGCCTGAAGTGCCTGGCCCGGCGCTTCATCAGCCAGTACCTGGAACTGACGGGGGATTACGAAGGCCTGGaa                                                                                            >  6:71049/1‑149 (MQ=255)
                                                         ttCAACGAGCCTTTCCGACTGACCGACGTCTACGCCGACACCGCCTTCCTGGCCATGGACCTGGAAGACCGCGGCCTGAAGTGCCTGGCCCGGCGCTTCATCAGCCAGTACCTGGAACTGACGGGGGATTACGAAGGCCTGGAACTGCt                                                                                       <  4:119816/149‑1 (MQ=255)
                                                           cAACTAGCCTTTCCCACTCCCCCAGGTCTACGCCTACACCGCTTTCGTGCCAATGAACCTGTAATACCGCGGCCGTAATTGCCTGGCCCGGCGCTTCATCAGCCAGTACCTGGAACTGACGGGGGATTACGAAGGCCTGGAACTGCTc                                                                                      <  1:94284/148‑1 (MQ=255)
                                                            aaCGAGCCTTTCCGACTGACCGACGTCTACGCCGACACCGCCTTCCTGGCCATGGACCTGGAAGACCGCGGCCTGAAGTGCCTGGCCCGGCGCTTCATCAGCCAGTACCTGGAACTGACGGGGGATTACGAAGGCCTGGAACTGCTCaa                                                                                    <  1:80750/149‑1 (MQ=255)
                                                            aaCGAGCCTTTCCGACTGACCGACGTCTACGCCGACACCGCCTTCCT‑GCCATGGACCTGGAAGACCGCGGCCTGAAGTGCCTGGCCCGGCGCTTCATCAGCCAGTACCTGGAACTGACGGGGGATTACGAAGGCCTGGAACTGCTCaa                                                                                    <  1:237657/148‑1 (MQ=255)
                                                             aCGAGCCTTTCCGACTGACCGACGTCTACGCCGACACCGCCTTCCTGGCCATGGACCTGGAAGACCGCGGCCTGAAGTGCCTGGCCCGGCGCTTCATCAGCCAGTACCTGGAACTGACGGGGGATTACGAAGGCCTGGAACTGCTCAAc                                                                                   <  1:220116/149‑1 (MQ=255)
                                                             aCGAGCCTTTCCGACTGACCGACGTCTACGCCGACACCGCCTTCCTGGCCATGGACCTGGAAGACCGCGGCCTGAAGTGCCTGGCCCGGCGCTTCATCAGCCAGTACCTGGAACTGACGGGGGATTACGAAGGCCTGGAACTGCTCAAc                                                                                   <  7:2480/149‑1 (MQ=255)
                                                                aGCCTTTCCGACTGACCGACGGCTACGCCGACACCGCCTTCCTGGCCATGGACCTGGAAGACCGCGGCCTGAAGTGCCTGGCCCGGCGCTTCATCAGCCAGTACCTGGAACTGACGGGGGATTACGAAGGCCTGGAACTGCTCAACTTc                                                                                <  6:68045/149‑1 (MQ=255)
                                                                    tttCCGACTGACCGACGTCTACGCCGACACCGCCTTCCTGGCCATGGACCTGGAAGACCGCGGCCTGAAGTGCCTGGCCCGGCGCTTCATCAGCCAGTACCTGGAACTGACGGGGGATTACGAAGGCCTTGAACTGCt                                                                                       <  2:1709/138‑1 (MQ=255)
                                                                    tttCCGACTGACCGACGTCTACGCCGACACCGCCTTCCTGGCCATGGACCTGGAAGACCGCGGCCTGAAGTGCCTGGCCCGGCGCTTCATCAGCCAGTACCTGGAACTGACGGGGGATTACGAAGGCCTGGAACTGCt                                                                                       >  1:1709/1‑138 (MQ=255)
                                                                       ccGACTGACCGACGTCTACGCCGACACCGCCTTCCTGGCCATGGACCTGGAAGACCGCGGCCTGAAGTGCCTGGCCCGGCGCTTCATCAGCCAGTACCTGGAACTGACGGGGGATTACGAAGGCCTGGAACTGCTCAACTTCTACaaa                                                                          <  3:351537/148‑1 (MQ=255)
                                                                              aCCGACGTCTACGCCGACACCGCCTTCCTGGCCATGGACCTGGAAGACCGCGGCCTGAAGTGCCTGGCCCGGCGCTTCATCAGCCAGTACCTGGAACTGACGGGGGATTACGAAGGCCTGGAACTGCTCAACTTCTACAAAGCCTAcc                                                                   <  3:278806/148‑1 (MQ=255)
                                                                                                  cGCCTTCCTGGCCATGGACCTGGAAGACCGCGGCCTGAAGTGCCTGGCCCGGCGCTTCATCAGCCAGTACCTGGAACTGACGGGGGATTACGAAGGCCTGGAACTGCTCAACTTCTACAAAGCCTACCGCGCGCTCGTACGGGCCAAgg                                              >  5:303470/1‑149 (MQ=255)
                                                                                                   gCCTTCCTGGCCATGGACCTTGAAGACCGCGGCCTGAAGTGCCTGGCCCGGCGCTTCATCAGCCAGTACCTGGAACTGACGGGGGATTACGaa                                                                                                     <  4:181612/93‑1 (MQ=255)
                                                                                                   gCCTTCCTGGCCATGGACCTGGAAGACCGCGGCCTGAAGTGCCTGGCCCGGCGCTTCATCAGCCAGTACCTGGAACTGACGGGGGATTACGaa                                                                                                     >  3:181612/1‑93 (MQ=255)
                                                                                                              cATGGACCTGGAAGACCGCGGCCTGAAGTGCCTGGCCCGGCGCTTCATCTGCCAGTACCTGGAACTGACGGGGGATGACGAAGGCCTGGAACTGCTCAAGTTCTACAAAGCCTACCGCGCGCGCGTACTGGCCAAGGTCGCAtt                                       <  7:140276/144‑1 (MQ=255)
                                                                                                              cATGGACCTGGAAGACCGCGGCCTGAAGTGCCTGGCCCGGCGCTTCATCAGCCAGTACCTGGAACTGACGGGGGATTACGAAGGCCTGGAACTGCTCAACTTCTACAAAGACTACCGCGCGCTCGTACGGGCCAAGGTCCCAtt                                       >  8:140276/1‑144 (MQ=255)
                                                                                                                 ggACCTGGAAGACCGCGGCCTGAAGTGCCTGGCCCGGCGCTTCATCAGCCAGTACCTGGAACTGACGGGGGATTACGAAGGCCTGGAACTGCTCAACTTCTACAAAGCCTACCGCGCGCTCGTACGGGCCAAGGTCGCAt                                        >  2:175221/1‑140 (MQ=255)
                                                                                                                 ggACCTGGAAGACCGCGGCCTGAAGTGCCTGGCCCGGCGCTTCATCAGCCAGTACCTGGAACTGACGGGGGATTACGAAGGCCTGGAACTGCTCAACTTCTACAAAGCCTACCGCGCGCTCGTACGGGCCAAGGTCGCAt                                        >  4:77883/1‑140 (MQ=255)
                                                                                                                 ggACCTGGAAGACCGCGGCCTGAAGTGCCTGGCCCGGCGCTTCATCAGCCAGTACCTGGAACTGACGGGGGATTACGAAGGCCTGGAACTGCTCAACTTCTACAAAGCCTACCGCGCGCTCGTACGGGCCAAGGTCGCAt                                        <  3:77883/140‑1 (MQ=255)
                                                                                                                 ggACCTGGAAGACCGCGGCCTGAAGTGCCTGGCCCGGCGCTTCATCAGCCAGTACCTGGAACTGACGGGGGATTACGAAGGCCTGGAACTGCTCAACTTCTACAAAGCCTACCGCGCGCTCGTACGGGCCAAGGTCGCAt                                        <  1:175221/140‑1 (MQ=255)
                                                                                                                      tGGAAGACCGCGGCCTGAAGTGCCTGGCCCGGCGCTTCATCAGCCAGTACCTGGAACTGACGGGGGATTACGAAGGCCTGGAACTGCTCAACTTCTACAAAGCCTACCGCGCGCTCGTACGGGCCAAGGTCGCATTGTTc                                   <  1:23167/140‑1 (MQ=255)
                                                                                                                      tGGAAGACCGCGGCCTGAAGTGCCTGGCCCGGCGCTTCATCAGCCAGTACCTGGAACTGACGGGGGATTACGAAGGCCTGGAACTGCTCAACTTCTACAAAGCCTACCGCGCGCTCGTACGGGCCAAGGTCGCATTGTTc                                   >  2:23167/1‑140 (MQ=255)
                                                                                                                       ggAAGACCGCGGCCTGAAGTGCCTGGCCCGGCGCTTCATCAGCCAGTACCTGGAACTGACGGGGGATTACGAAGGCCTGGAACTGCTCAACTTCTACAAAGCCTACCGCGCGCTCGTACGGGCCAAGGTCGCATTGTTCAGCATgccgg                         >  5:125235/1‑149 (MQ=255)
                                                                                                                              cgcgGCCTGAAGTGCCTGGCCCGGCGCTTCATCAGCCAGTACCTGGAACTGACGGGGGATTACGAAGGCCTGGAACTGCTCAACTTCTACAAAGCCTACCGCGCGCTCGTACGGGCCAAGGTCGCATTGt                                     <  6:282108/130‑1 (MQ=255)
                                                                                                                              cgcgGCCTGAAGTGCCTGGCCCGGCGCTTCATCAGCCAGTACCTGGAACTGACGGGGGATTACGAAGGCCTGGAACTGCTCAACTTCTACAAAGCCTACCGCGCGCTCGTACGGGCCAAGGTCGCATTGt                                     >  5:282108/1‑130 (MQ=255)
                                                                                                                                 ctgatGAAGCGCCGGGCCAGGCACTTCATCAGCCAGTACCTGGAACTGACGGGGGATTACGAAGGCCTGGAACTGCTCAACTTCTACAAAGCCTACCGCGCGCTCGTACGGGCCAAGGTCGCATTGTTCAGCATGCCGGCCGACGCTGa               >  2:18580/5‑149 (MQ=255)
                                                                                                                                    atGAAGCGCCGGGCCAGGCACTTCATCAGCCAGTACCTGGAACTGACGGGGGATTACGAAGGCCTGGAACTGCTCAACTTCTACAAAGCCTACCGCGCGCTCGTACGGGCCAAGGTCGCATTGTTCAGCATGCCGGCCGACGCTGATgg            >  7:27611/2‑149 (MQ=255)
                                                                                                                                       aaGTGCCTGGCCCGGCGCTTCATCAGCCAGTACCTGGAACTGACGGGGGATTACGAAGGCCTGGAACTGCTCAACTTCTACAAAGCCTACCGCGCGCTCGTACGGGCCAAGGTCGCATTGTTCAGCATGCCGGCCGACGCTGATGGCGt         >  1:202048/1‑149 (MQ=255)
                                                                                                                                            ccTGGCCCGGCGCTTCATCAGCCAGTACCTGGAACTGACGGGGGATTACGAAGGCCTGGAACTGCTCAACTTCTACAAAGCCTACCGCGCGCTCGTACGGGCCAAGGTCGCATTGTTCAGCATGCCGGCCGACGCTGATGGCGTTCAGc    >  8:152265/1‑149 (MQ=255)
                                                                                                                                                gCCCGGCGCTTCATCAGCCAGTACCTGGAACTGACGGGGGATTACGAAGGCCTGGAACTGCTCAACTTCTACAAAGCCTACCGCGCGCTCGTACGGGCCAAGGTCGCATTGTTCAGCATGCCGGCCGACGCTGATGGCGTTCAGCGt  <  5:71049/147‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                               
CACCTGGGCAACGCCACCTTGATCGATGGCAAGGTGGTGATCTTCGATTGCATCGAGTTCAACGAGCCTTTCCGACTGACCGACGTCTACGCCGACACCGCCTTCCTGGCCATGGACCTGGAAGACCGCGGCCTGAAGTGCCTGGCCCGGCGCTTCATCAGCCAGTACCTGGAACTGACGGGGGATTACGAAGGCCTGGAACTGCTCAACTTCTACAAAGCCTACCGCGCGCTCGTACGGGCCAAGGTCGCATTGTTCAGCATGCCGGCCGACGCTGATGGCGTTCAGCGT  >  NC_002947/5324015‑5324305

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑