Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,339,897 C→G 10.7% R375R (CGC→CGG) ‡ pcnB → poly(A) polymerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,339,8970CG10.7% 160.8 / 10.9 56R375R (CGC→CGG) ‡pcnBpoly(A) polymerase
Reads supporting (aligned to +/- strand):  ref base C (26/24);  new base G (3/3);  total (29/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.34e-01

CCTGCAGAACCAGGGCGTGCCGCCGATCCCGGCCATGAACGGTGCGGCCCACGACCTGATCGCCGAGCAGTGCGCGCGCATTGCCATCCCCAAGCGTTTTACCCTGCCGATCCGCGAGATCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCCTGCTGCTGCGTGAAAGCGCCGGGGAAGAAACCGACGAACTGGGCCAGTGGTGGACCGATTACCAGGACGCAAACGACA  >  NC_002947/5339750‑5340039
                                                                                                                                                   |                                                                                                                                              
ccTGCAGAACCAGGGCGTGCCGCCGATCCCGGCCATGAACGGTGCGGCCCACGACCTGATCGCCGAGCAGTGCGCGCGCATTGCCATCCCCAAGCGTTTTACCCTGCCGATCCGCGAGATCTGGGACATGCAGGAGCGCCTgccgcgcc                                                                                                                                               <  5:216817/149‑1 (MQ=255)
   gCAGAACCAGGGCGTGCCGCCGATCCCGGCCATGAACGGTGCGGCCCACGACCTGATCGCCGAGCAGTGCGCGCGCATTGCCATCCCCAAGCGTTTTACCCTGCCGATCCGCGAGATCTGGGACATGCAGGAGCGCCTGCCGCGCCGCa                                                                                                                                            >  6:343289/1‑149 (MQ=255)
     aGAACCAGGGCGTGCCGCCGATCCCGGCCATGAACGGTGCGGCCCACGACCTGATCGCCGAGCAGTGCGCGCGCATTGCCATCCCCAAGCGTTTTACCCTGCCGATCCGCGAGATCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGc                                                                                                                                          <  6:104412/149‑1 (MQ=255)
      gAACCAGGGCGTGCCGCCGATCCCGGCCATGAACGGTGCGGCCCACGACCTGATCGCCGAGCAGTGCGCGCGCATTGCCATCCCCAAGCGTTTTACCCTGCCGATCCGCGAGATCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCg                                                                                                                                         <  5:148430/149‑1 (MQ=255)
          cAGGGCGTGCCGCCGATCCCGGCCATGAACGGTGCGGCCCACGACCTGATCGCCGAGCAGTGCGCGCGCATTGCCATCCCCAAGCGTTTTACCCTGCCGATCCGCGAGATCTGGGACATGCAGGAGCGCTTGCCGCTGCGGCGCGGCag                                                                                                                                     >  6:97273/1‑148 (MQ=255)
            gggCGTGCCGCCGATCCCGGCCATGAACGGTGCGGCCCACGACCTGATCGCCGAGCAGTGCGCGCGCATTGCCATCCCCAAGCGTTTTACCCTGCCGATCCGCGAGATCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCaatc                                                                                                                                   <  2:316643/149‑3 (MQ=255)
                gTGCCGCCGATCCCGGCCATGAACGGTGCGGCCCACGACCTGATCGCCGAGCAGTGCGCGCGCATTGCCATCCCCAAGCGTTTTACCCTGCCGATCCGCGAGATCTGGGACATGCAGGAGCGCTTGCCGCTGCGGCGCGGCAGgcgctc                                                                                                                               <  5:97273/149‑3 (MQ=255)
                     gccgATCCCGGCCATGAACGGTGCGGCCCACGACCTGATCGCCGAGCAGTGCGCGCGCATTGCCATCCCCAAGCGTTTTACCCTGCCGATCCGCGAGATCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACa                                                                                                                          <  7:80712/149‑1 (MQ=255)
                            ccGGCCATGAACGGTGCGGCCCACGACCTGATCGCCGAGCAGTGCGCGCGCATTGCCATCCCCAAGCGTTTTACCCTGCCGATCCGCGAGATCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATgctgct                                                                                                                   <  7:263490/149‑1 (MQ=255)
                             cGGCCATGAACGGTGCGGCCCACGACCTGATCGCCGAGCAGTGCGCGCGCATTGCCATCCCCAAGCGTTTTACCCTGCCGATCCGCGAGATCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGctgctg                                                                                                                  >  1:312726/1‑149 (MQ=255)
                                  aTGAACGGTGCGGCCCACGACCTGATCGCCGAGCAGTGCGCGCGCATTGCCATCCCCAAGCGTTTTACCCTGCCGATCCGCGAGATCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACaa                                                                                                             >  2:128304/1‑149 (MQ=255)
                                       cGGTGCGGCCCACGACCTGATCGCCGAGCAGTGCGCGCGCATTGCCATCCCCAAGCGTTTTACCCTGCCGATCCGCGAGATCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCcgc                                                                                                        <  5:364901/149‑1 (MQ=255)
                                        ggTGCGGCCCACGACCTGATCGCCGAGCAGTGCGCGCGCATTGCCATCCCCAAGCGTTTTACCCTGCCGATCCGCGAGATCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCcgcg                                                                                                       >  5:121423/1‑149 (MQ=255)
                                             ggCCCACGACCTGATCGCCGAGCAGTGCGCGCTCATTGCCATCCCCAAGCGTTTTACCCTGCCGATCCGGGAGATCTGGGACATGCATGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTcc                                                                                                  <  3:283929/149‑1 (MQ=255)
                                             ggCCCACGACCTGATCGCCGAGCAGTGCGCGCGCATTGCCATCCCCAAGCGTTTTACCCTGCCGATCCGCGAGATCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTcc                                                                                                  >  3:182216/1‑149 (MQ=255)
                                             ggCCCACGACCTGATCGCCGAGCAGTGCGCGCGCATTGCCATCCCCAAGCGTTTTACCCTGCCGATCCGCGAGATCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTcc                                                                                                  <  1:105622/149‑1 (MQ=255)
                                                       cTGATCGCCGAGCAGTGCGCGCGCATTGCCATCCCCAAGCGTTTTACCCTGCCGATCCGCGAGATCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTa                                                                                        <  8:174853/149‑1 (MQ=255)
                                                         gATCGCCGAGCAGTGCGCGCGCATTGCCATCCCCAAGCGTTTTACCCTGCCGATCCGCGAGATCTGGGACATGCAGGCGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACg                                                                                      >  2:148374/1‑149 (MQ=255)
                                                         gATCGCCGAGCAGTGCGCGCGCATTGCCATCCCCAAGCGTTTTACCCTGCCGATCCGCGAGATCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGctgctg                                                                                                                  <  6:136287/121‑1 (MQ=255)
                                                         gATCGCCGAGCAGTGCGCGCGCATTGCCATCCCCAAGCGTTTTACCCTGCCGATCCGCGAGATCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGctgctg                                                                                                                  >  5:136287/1‑121 (MQ=255)
                                                              ccGAGCAGTGCGCGCGCATTGCCATCCCCAAGCGTTTTACCCTGCCGATCCGCGAGATCTGGGACATGCAGGAGCGCCTGCCGCTGCGGCGCGGCag                                                                                                                                     <  3:161353/97‑2 (MQ=255)
                                                              ccGAGCAGTGCGCGCGCATTGCCATCCCCAAGCGTTTTACCCTGCCGATCCGCGAGATCTGGGACATGCAGGAGCGCCTGCCGCTGCGGCGCGGCag                                                                                                                                     >  4:161353/1‑96 (MQ=255)
                                                              ccGAGCAGTGCGCGCGCATTGCCATCCCCAAGCGTTTTACCCTGCCGATCCGCGAGATCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTc                                                                                 <  8:171906/149‑1 (MQ=255)
                                                              ccGAGCAGTGCGCGCGCATTGCCATCCCCAAGCGTTTTACCCTGCCGATCCGCGAGATCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTc                                                                                 <  5:343289/149‑1 (MQ=255)
                                                                gAGCAGTGCGCGCGCATTGCCATCCCCAAGCGTTTTACCCTGCCGATCCGCGAGATCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCct                                                                               <  2:312726/149‑1 (MQ=255)
                                                                 accAGTGCGCGCGCATTGCCATCCCCAAGCGTTTTACCCTGCCGATCCGCGAGATCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTcc                                                                                <  1:128304/145‑1 (MQ=255)
                                                                      tGCGCGCGCATTGCCATCCCCAAGCGTTTTACCCTGCCGATCCGCGAGATCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCctgctgct                                                                         >  5:274827/1‑149 (MQ=255)
                                                                           gcgcATTGCCATCCCCAAGCGTTTTACCCTGCCGATCCGCGAGATCTCGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCCTGCTGCTGCGt                                                                     >  1:202156/1‑148 (MQ=255)
                                                                              cATTGCCATCCCCAAGCGTTTTACCCTGCCGATCCGCGAGATCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCCTGCTGCTGCGTGaaa                                                                 <  2:202156/149‑1 (MQ=255)
                                                                                     aTCCCCAAGCGTTTTACCCTGCCGATCCGCGAGATCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCCTGCTGCTGCGTGAAAGCGCCgg                                                          >  6:112274/1‑149 (MQ=255)
                                                                                                   tACCCTGCCGATCCGCGAGATCTGGTGCATGCATGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATTCTTCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCctgct                                                                            <  7:290753/117‑1 (MQ=255)
                                                                                                   tACCCTGCCGATCCGCGAGATCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCCTGCTGCTGCGTGAAAGCGCCGGGGAAGAAACcgac                                             <  6:121423/148‑1 (MQ=255)
                                                                                                   tACCCTGCCGATCCGCGAGATCTGCGGCATGCAGGAGCGACTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTcctcct                                                                            >  8:290753/1‑117 (MQ=255)
                                                                                                      ccTGCCGATCCGCGAGATCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTc                                                                                                   >  8:167667/1‑91 (MQ=255)
                                                                                                      ccTGCCGATCCGCGAGATCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTc                                                                                                   <  7:167667/91‑1 (MQ=255)
                                                                                                         gCCGATCCGCGAGATCTGGGACATGCAGGAGCGCCTGCCGCGCAGCAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGATTCCGCGCCGGTTACGACTTCCTGCTGCTGCGTGAAAGCGCCGGGGAAGAAACCGACGAACTgg                                      >  5:144632/1‑149 (MQ=255)
                                                                                                            gATCCGCGAGATCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCCTGCTGCTGCGTGAAAGCGCCGGGGAAGAAACCGACGAACTGGGc                                    >  8:203171/1‑148 (MQ=255)
                                                                                                                cgcgAGATCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCCTGCTGCTGCGTGAAAGCGCCGGGGAAGAAACCGACGAACTGGGCCAgtg                               >  4:108835/1‑149 (MQ=255)
                                                                                                                 gcgAGATCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCCTGCTGCTGCGTGAAAGCGCCGGGGAAGAAACCGACGAACTGGGCCAgtgg                              >  6:282324/1‑149 (MQ=255)
                                                                                                                  cgAGATCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCCTGCTGCTGCGTGAAAGCGCCGGGGAAGAAACCGACGAACTGGGCCAgtggt                             >  4:340562/1‑149 (MQ=255)
                                                                                                                  cgAGATCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCCTGCTGCTGCGTGAAAGCGCCGGGGAAGAAACCGACGAACTGGGCCAgtggt                             >  5:344164/1‑149 (MQ=255)
                                                                                                                   gagaTCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCCTGCTGCTGCGTGAAAGCGCCGGGGAAGAAACCGACGAACTGGGCCAgtggtg                            >  7:240891/1‑149 (MQ=255)
                                                                                                                    agaTCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCCTGCTGCTGCGTGAAAGCGCCGGGGAAGAAACCGACGAACTGGGCCAgtggtg                            >  3:329592/1‑148 (MQ=255)
                                                                                                                       tCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCCTGCTGCTGCGTGAAAGCGCCGGGGAAGAAACCGACGAACTGGGCCAGTGGTGGAcc                        >  2:79164/1‑149 (MQ=255)
                                                                                                                         tGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCCTGCTGCTGCGTGAAAGCGCCGGGGAAGAAACCGACGAACTGGGCCAGTGGTGGACCGa                      >  4:108583/1‑149 (MQ=255)
                                                                                                                            gACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCCTGCTGCTGCGTGAAAGCGCCGGGGAAGAAACCGACGAACTGGGCCAGTGGTGGACCGATTa                   <  6:274827/149‑1 (MQ=255)
                                                                                                                                   aGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCCTGCTGCTGCGTGAAAGCGCCGGGGAAGAAACCGACGAACTgg                                      >  3:265854/1‑123 (MQ=255)
                                                                                                                                   aGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCCTGCTGCTGCGTGAAAGCGCCGGGGAAGAAACCGACGAACTgg                                      <  4:265854/123‑1 (MQ=255)
                                                                                                                                   aGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCCTGCTGCTGCGTGAAAGCGCCGGGGAAGAAACCGACGAACTGGGCCAGTGGTGGACCGATTACCAGGAc            <  5:112274/149‑1 (MQ=255)
                                                                                                                                     gcgcgcTTGCCGCTGCGGCGCGGCAGGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCCTGCTGCTGCGTGAAAGCGCCGGGga                                                       >  4:207014/3‑104 (MQ=37)
                                                                                                                                     gcgcgcTTGCCGCTGCGGCGCGGCAGGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCCTGCTGCTGCGTGAAAGCGCCGGGga                                                       <  3:207014/102‑1 (MQ=37)
                                                                                                                                          ccTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCCTGCTGCTGCGTGAAAGCGCCgg                                                          >  8:113605/1‑96 (MQ=255)
                                                                                                                                          ccTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCCTGCTGCTGCGTGAAAGCGCCgg                                                          <  7:113605/96‑1 (MQ=255)
                                                                                                                                          ccTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCCTGCTGCTGCGTGAAAGCGCCGGGGAAGAAACCGACGAACTGGGCCAGTGGTGGACCGATTACCAGGACGCAAAc      >  2:230051/1‑148 (MQ=255)
                                                                                                                                          ccTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCCTGCTGCTGCGTGAAAGCGCCGGGGAAGAAACCGACGAACTGGGCCAGTGGTGGACCGATTACCAGGACGCAAACg     <  4:329592/149‑1 (MQ=255)
                                                                                                                                           cTGCCGCGCCGCAGCGGCAAGCGCGCCTACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCCTGCTTCTGCGTGAAAGCGCCGGGGAAGAAACCGACGAACTGGGCCAGTGGTGGACCGATTACCAGGACGCAAACGa    <  4:182216/149‑1 (MQ=255)
                                                                                                                                           cTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCCTGCTGCTGCGTGAAAGCGCCGGGGAAGAAACCGACGAACTGGGCCAGTGGTGGACCGATTACCAGGACGCAAACGa    >  7:45582/1‑149 (MQ=255)
                                                                                                                                              ccgcgccgcAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCCTGCTGCTGCGTGAAAGCGCCGGGGAAGAAACCGACGAACTGGGCCAGTGGTGGACCGATTACCAGGACGCAAACGACa  <  7:203171/148‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                              
CCTGCAGAACCAGGGCGTGCCGCCGATCCCGGCCATGAACGGTGCGGCCCACGACCTGATCGCCGAGCAGTGCGCGCGCATTGCCATCCCCAAGCGTTTTACCCTGCCGATCCGCGAGATCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGCGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCCTGCTGCTGCGTGAAAGCGCCGGGGAAGAAACCGACGAACTGGGCCAGTGGTGGACCGATTACCAGGACGCAAACGACA  >  NC_002947/5339750‑5340039

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑