Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,348,352	A→G	6.8%	K276E (AAG→GAG)	PP_4704 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,348,352	0	A	G	6.8%	170.2 / 2.6	59	K276E (AAG→GAG)	PP_4704	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (32/23);  new base G (2/2);  total (34/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.01e-01

CTGTTCTGGCCCTACGTGCGCGATGCCGTGCCACTGGTGCTGGAAGACGGCGTGGTAAGCCTCGATACGCATTACAAGCTCAACCTCGCCAAACAGACCGAGCTGCTGCTGGATAACGTCTCCGTGCGCATCGCGCCATTCGCCATCAAGGCGCCGGATGGCCGACCGTTGGCACGTCT‑‑‑‑GG‑CCAGCCTGGAGGTTAGCGAAACCTCCATCGATCTGGTCAAGCAGCAGGTCACCGTGGGCAAGGTTCGCAGCGAAAAGCTCGAAACCTGGGCGGCGCTGGAAAAAGATGGC  >  NC_002947/5348205‑5348495                                                                                                                                                    |                                                                                                                                                     cTGTTCTGGCCCTACGTGCGCGATGCCGTGCCACTGGTGCTGGAAGACGGCGTGGTAAGCCTCGATACTCATTACAAGCTCAACCTCGCCAAACAGACCGAGCTGCTGCTGGATAACGTCTCCGTGCGCATCGCGCCATTCGCCATCaa                                                                                                                                                     <  3:47185/149‑1 (MQ=255)           ccTACGTGCGCGATGCCGTGCCACTGGTGCTGGAAGACGGCGTGGTAAGCCTCGATACGCATTACAAGCTCAACCTCGCCAAACAGACCGAGCTGCTGCTGGATAACGTCTCCGTGCGCATCGCGCCATTCGCCATCAAGGCGCCGGAt                                                                                                                                           >  8:247028/1‑149 (MQ=255)               cGTGCGCGATGCCGTGCCACTGGTGCTGGAAGACGGCGTGGTAAGCCTCGATACGCATTACAAGCTCAACCTCGCCAAACAGACCGAGCTGCTGCTGGATAACGTCTCCGTGCGCATCGCGCCATTCGCCATCAAGGCGCCGGATgg                                                                                                                                         <  6:157572/147‑1 (MQ=255)                      gATGCCGTGCCACTGGTGCTGGAAGACGGCGTGGTAAGCCTCGATACGCATTACAAGCTCAACCTCGCCAAACAGACCGAGCTGCTGCTGGATAACGTCTCCGTGCGCATCGCGCCATTCGCCATCAAGGCGCCGGATGGCCGACCGtt                                                                                                                                >  7:241823/1‑149 (MQ=255)                          ccGTGCCACTGGTGCTGGAAGACGGCGTGGTAAGCCTCGATACGCATTACAAGCTCAACCTCGCCAAACAGACCGAGCTGCTGCTGGATAACGTCTCCGTGCGCATCGCGCCATTCGCCATCAAGGCGCCGGATGGCCGACCGTTGGc                                                                                                                             <  6:59632/148‑1 (MQ=255)                                cACTGGTGCTGGAAGACGGCGTGGTAAGCCTCGATACGCATTACAAGCTCAACCTCGCCAAACAGACCGAGCTGCTGCTGGATAACGTCTCCGTGCGCATCGCGCCATTCGCCATCAAGGCGCCGGATGGCCGACCGTTGGCACGTCT‑‑‑‑g                                                                                                                  <  1:172338/149‑1 (MQ=255)                                cACTGGTGCTGGAAGACGGCGTGGTAAGCCTCGATACGCATTACAAGCTCAACCTCGCCAAACAGACCGAGCTGCTGCTGGATAACGTCTCCGTGCGCATCGCGCCATTCGCCATCAAGGCGCCGGATGGCCGACCGTTGGCACGTCT‑‑‑‑g                                                                                                                  >  5:9974/1‑149 (MQ=255)                                        cTGGAAGACGGCGTGGTAAGCCTCGATACGCATTACAAGCTCAACCTCGCCAAACAGACCGAGCTGCTGCTGGATAACGTCTCCGTGCGCATCGCGCCATTCGCCATCAAGGCGCCGGATGGCCGACCGTTGGCACGTCT‑‑‑‑GG‑CCAGcc                                                                                                          <  8:241823/148‑1 (MQ=255)                                          ggAAGACGGCGTGGTAAGCCTCGATACGCATTACAAGCTCAACCTCGCCAAACAGACCGAGCTGCTGCTGGATAACGTCTCCGTGCGCATCGCGCCATTCGCCATCAAGGCGCCGGATGGCCGACCGTTGGCACGTCT‑‑‑‑GG‑CCAGCCTg                                                                                                        >  7:187870/1‑148 (MQ=255)                                           gAAGACGGCGTGGTAAGCCTCGATACGCATTACAAGCTCAACCTCGCCAAACAGACCGAGCTGCTGCTGGATAACGTCTCCGTGCGCATCGCGCCATTCGCCATCAAGGCGCCGGATGGCCGACCGTTGGCACGTCT‑‑‑‑GG‑CCAGCCTg                                                                                                        >  7:343394/1‑147 (MQ=255)                                           gAAGACGGCGTGGTAAGCCTCGATACGCATTACAAGCTCAACCTCGCCAAACAGACCGAGCTGCTGCTGGATAACGTCTCCGTGCGCATCGCGCCATTCGCCATCAAGGCGCCGGATGGCCGACCGTTGGCACGTCT‑‑‑‑GG‑CCAGCCTGGa                                                                                                      <  3:111317/149‑1 (MQ=255)                                            aaGACGGCGTGGTAAGCCTCGATACGCATTACAAGCTCAACCTCGCCAAACAGACCGAGCTGCTGCTGGATAACGTCTCCGTGCGCATCGCGCCATTCGCCATCAAGGCGCCGGATGGCCGACCGTTGGCACGTCT‑‑‑‑GG‑CCAGCCTGGAg                                                                                                     <  6:89902/149‑1 (MQ=255)                                              gACGGCGTGGTAAGCCTCGATACGCATTACAAGCTCAACCTCGCCAAACAGACCGAGCTGCTGCTGGATAACGTCTCCGTGCGCATCGCGCCATTCGCCATCAAGGCGCCGGATGGCCGACCGTTGGCACGTCT‑‑‑‑GG‑CCAGCCTGGAGGt                                                                                                   <  2:282161/149‑1 (MQ=255)                                                    gTGGTAAGCCTCGATACGCATTACAAGCTCAACCTCGCCAAACAGACCGAGCTGCTGCTGGATAACGTCTCCGTGCGCATCGCGCCATTCGCCATCAAGGCGCCGGATGGCCGACCGTTGGCACGTCT‑‑‑‑GG‑CCAGCCTGGAg                                                                                                     >  6:321989/1‑141 (MQ=255)                                                    gTGGTAAGCCTCGATACGCATTACAAGCTCAACCTCGCCAAACAGACCGAGCTGCTGCTGGATAACGTCTCCGTGCGCATCGCGCCATTCGCCATCAAGGCGCCGGATGGCCGACCGTTGGCACGTCT‑‑‑‑GG‑CCAGCCTGGAg                                                                                                     <  5:321989/141‑1 (MQ=255)                                                             cTCGATACGCATTACAAGCTCAACCTCGCCAAACAGACCGAGCTGCTGCTGGATAACGTCTCCGTGCGCATCGCGCCATTCGCCATCAAGGCGCCGGATGGCCGACCGTTGGCACGTCT‑‑‑‑GG‑CCAGCCTGGAg                                                                                                     >  3:61451/1‑132 (MQ=255)                                                             cTCGATACGCATTACAAGCTCAACCTCGCCAAACAGACCGAGCTGCTGCTGGATAACGTCTCCGTGCGCATCGCGCCATTCGCCATCAAGGCGCCGGATGGCCGACCGTTGGCACGTCT‑‑‑‑GG‑CCAGCCTGGAg                                                                                                     <  4:61451/132‑1 (MQ=255)                                                             cTCGATACGCATTACAAGCTCAACCTCGCCAAACAGACCGAGCTGCTGCTGGATAACGTCTCCGTGCGCATCGCGCCATTCGCCATCAAGGCGCCGGATGGCCGACCGTTGGCACGTCT‑‑‑‑GG‑CCAGCCTGGAGGTTa                                                                                                 <  7:330672/136‑1 (MQ=255)                                                             cTCGATACGCATTACAAGCTCAACCTCGCCAAACAGACCGAGCTGCTGCTGGATAACGTCTCCGTGCGCATCGCGCCATTCGCCATCAAGGCGCCGGATGGCCGACCGTTGGCACGTCT‑‑‑‑GG‑CCAGCCTGGAGGTTa                                                                                                 >  8:330672/1‑136 (MQ=255)                                                                      cATTACAAGCTCAACCTCGCCAAACAGACCGAGCTGCTGCTGGATAACGTCTCCGTGCGCATCGCGCCATTCGCCATCAAGGCGCCGGATGGCCGACCGTTGGCACGTCT‑‑‑‑GG‑CCAGCCTGGAGGTTAGCGAAACCTCCATCGATCTGGt                                                                           <  7:247028/149‑1 (MQ=255)                                                                            aaGCTCAACCTCGCCAAACAGACCGAGCTGCTGCTGGATAACGTCTCCGTGCGCATCGCGCCATTCGCCATCGAGGCGCCGGATgg                                                                                                                                         >  2:242864/1‑86 (MQ=255)                                                                            aaGCTCAACCTCGCCAAACAGACCGAGCTGCTGCTGGATAACGTCTCCGTGCGCATCGCGCCATTCGCCATCGAGGCGCCGGATgg                                                                                                                                         <  1:242864/86‑1 (MQ=255)                                                                            aaGCTCAACCTCGCCAAACAGACCGAGCTGCTGCTGGATAACGTCTCCGTGCGCATCGCGCCATTCGCCATCAAGGCGCCGGATGGCCGACCGTTGGCACGTCT‑‑‑‑GG‑CCAGCCTGGAGGt                                                                                                   >  5:11752/1‑119 (MQ=255)                                                                            aaGCTCAACCTCGCCAAACAGACCGAGCTGCTGCTGGATAACGTCTCCGTGCGCATCGCGCCATTCGCCATCAAGGCGCCGGATGGCCGACCGTTGGCACGTCT‑‑‑‑GG‑CCAGCCTGGAGGt                                                                                                   <  6:11752/119‑1 (MQ=255)                                                                              gCTCAACCTCGCCAAACAGACCGAGCTGCTGCTGGATAACGTCTCCGTGCGCATCGCGCCATTCGCCATCAAGGCGCCGGATGGCCGACCGTTGGCACGTCT‑‑‑‑GG‑CCAGCCTGGAGGTTAGCGAAACCTCCATCGATCTg                                                                             <  4:163130/139‑1 (MQ=255)                                                                              gCTCAACCTCGCCAAACAGACCGAGCTGCTGCTGGATAACGTCTCCGTGCGCATCGCGCCATTCGCCATCAAGGCGCCGGATGGCCGACCGTTGGCACGTCT‑‑‑‑GG‑CCAGCCTGGAGGTTAGCGAAACCTCCATCGATCTg                                                                             >  3:163130/1‑139 (MQ=255)                                                                              gCTCAACCTCGCCAAACAGACCGAGCTGCTGCTGGATAACGTCTCCGTGCGCATCGCGCCATTCGCCATCAAGGCGCCGGATGGCCGACCGTTGGCACGTCT‑‑‑‑GG‑CCAGCCTGGAGGTTAGCGAAACCTCCATCGATCTGGTCAagcag                                                                    >  5:329394/1‑148 (MQ=255)                                                                                tCAACCTCGCCAAACAGACCGAGCTGCTGCTGGATAACGTCTCCGTGCGCATCGCGCCATTCGCCATCGAGGCGCCGGATGGCCGACCGTTGGCACGTCT‑‑‑‑GG‑CCAGCCTGGAGGTTAGCGAAACCTCCATCGATCTGGTCAAGcagcag                                                                 >  5:133754/1‑149 (MQ=255)                                                                                  aaCCTCGCCAAACAGACCGAGCTGCTGCTGGATAACGTCTCCGTGCGCATCGCGCCATTCGCCATCAAGGCGCCGGATGGCCGACCGTTGGCACGTCTCGGGGGTGCAGACTGGAGGTTAGCGAAACCTCCATCGATCTGCTCAagcag                                                                    >  1:120222/1‑149 (MQ=255)                                                                                  aaCCTCGCCAAACAGACCGAGCTGCTGCTGGATAACGTCTCCGTGCGCATCGCGCCATTCGCCATCAAGGCGCCGGATGGCCGACCGTTGGCACGTCT‑‑‑‑GG‑CCAGCCTGGAGGTTAGCGAAACCTCCATCGATCTGGTCAAGCAGCAGGt                                                               >  6:300332/1‑149 (MQ=255)                                                                                   aCCTCGCCAAACAGACCGAGCTGCTGCTGGATAACGTCTCCGTGCGCATCGCGCCATTCGCCATCAAGGCGCCGGATGGCCGACCGTTGGCACGTCT‑‑‑‑GG‑CCAGCCTGGAGGTTAGCGAAACCTCCATCGATCTGGTCAAGCAGCAGGt                                                               >  6:67168/1‑148 (MQ=255)                                                                                      tCGCCAAACAGACCGAGCTGCTGCTGGATAACGTCTCCGTGCGCATCGCGCCATTCGCCATCAAGGCGCCGGATGGCCGACCGTTGGCACGTCT‑‑‑‑GG‑CCAGCCTGGAGGTTAGCGAAACCTCCATCGATCTGGTCAAGCAGCAGGTCAcc                                                           >  5:3504/1‑149 (MQ=255)                                                                                          cAAACAGACCGAGCTGCTGCTGGATAACGTCTCCGTGCGCATCGCGCCATTCGCCATCAAGGCGCCGGATGGCCGACCGTTGGCACGTCT‑‑‑‑GG‑CCAGCCTGGAGGTTAGCGAAACCTCCATCGATCTGGTCAAGCAGCAGGTCACCGTgg                                                       <  6:329394/149‑1 (MQ=255)                                                                                            aaCAGACCGAGCTGCTGCTGGATAACGTCTCCGTGCGCATCGCGCCATTCGCCATCAAGGCGCCGGATGGCCGACCGTTGGCACGTCT‑‑‑‑GG‑CCAGCCTGGAGGTTAGCGAAACCTCCATCGATCTGGTCAAGCAGCAGGTCACCGTggg                                                      >  4:85925/1‑148 (MQ=255)                                                                                                 aCCGAGCTGCTGCTGGATAACGTCTCCGTGCGCATCGCGCCATTCGCCATCAAGGCGCCGGATGGCCGACCGTTGGCACGTCT‑‑‑‑GG‑CCAGCCTGGAGGTTAGCGAAACCGCCATCGATCTGGTCAAGCAGCAGGTCACCGTGGGCAAGGt                                                >  6:214715/1‑149 (MQ=255)                                                                                                     aGCTGCTGCTGGATAACGTCTCCGTGCGCATCGCGCCATTCGCCATCGAGGCGCCGGATGGCCGACCGTTGGCACGTCT‑‑‑‑GG‑CCAGCCTGGAGGTTAGCGAAACCTCCATCGATCTGGTCAAGCAGCAGGTCACCGTGGGCAAGGTTCGc                                            <  6:133754/149‑1 (MQ=255)                                                                                                        tgctgctgGATAACGTCTCCGTGCGCATCGCGCCATTCGCCATCAAGGCGCCGGATGGCCGACCGTTGGCACGTCT‑‑‑‑GG‑CCAGCCTgg                                                                                                       <  6:333741/87‑1 (MQ=255)                                                                                                        tgctgctgGATAACGTCTCCGTGCGCATCGCGCCATTCGCCATCAAGGCGCCGGATGGCCGACCGTTGGCACGTCT‑‑‑‑GG‑CCAGCCTgg                                                                                                       >  5:333741/1‑87 (MQ=255)                                                                                                          ctgctgGATAACGTCTCCGTGCGCATCGCGCCATTCGCCATCAAGGCGCCGGATGGCCGACCGTTGGCACGTCT‑‑‑‑GG‑CCAGCCTGGAGGTTAGCGAAACCTCCATCGATCTGGTCAAGCAGCAGGTCACCGTGGGCAAGGTTCGCAGCg                                        >  1:81287/1‑148 (MQ=255)                                                                                                               ggATAACGTCTCCGTGCGCATCGCGCCATTCGCCATCAAGGCGCCGGATGGCCGACCGTTGGCACGTCT‑‑‑‑GG‑CCAGCCTGGAGGTTAGCGAAACCTCCATCGATCTGGTCAAGCAGCAGGTCACCGTGGGCAAGGTTCGCAGCGAAAAGc                                  >  1:183111/1‑149 (MQ=255)                                                                                                                gATAACGTCTCCGTGCGCATCGCGCCATTCGCCATCAAGGCGCCGGATGGCCGACCGTTGGCACGTCT‑‑‑‑GG‑CCAGCCTGGAGGTTAGCGAAACCTCCATCGATCTGGTCAAGCAGCAGGTCACCGTgg                                                       >  6:358230/1‑127 (MQ=255)                                                                                                                gATAACGTCTCCGTGCGCATCGCGCCATTCGCCATCAAGGCGCCGGATGGCCGACCGTTGGCACGTCT‑‑‑‑GG‑CCAGCCTGGAGGTTAGCGAAACCTCCATCGATCTGGTCAAGCAGCAGGTCACCGTGGGCAAGGTTCGCAGCGAAAAGCt                                 >  3:314707/1‑149 (MQ=255)                                                                                                                gATAACGTCTCCGTGCGCATCGCGCCATTCGCCATCAAGGCGCCGGATGGCCGACCGTTGGCACGTCT‑‑‑‑GG‑CCAGCCTGGAGGTTAGCGAAACCGCCATCGATCTGGTCAAGCAGCAGGTCACCGTGGGCAAGGTTCGCAGCGAAAAGc                                  <  5:214715/148‑1 (MQ=255)                                                                                                                gATAACGTCTCCGTGCGCATCGCGCCATTCGCCATCAAGGCGCCGGATGGCAGACCGTTGGCACGTCT‑‑‑‑GG‑CCAGCCTGGAGGTTAGCGAAACCTCCATCGATCTGGTCAAGCAGCAGGTCACCGTgg                                                       <  5:358230/127‑1 (MQ=255)                                                                                                                   aaCGTCTCCGTGCGCATCGCGCCATTCGCCATCAAGGCGCCGGATGGCCGACCGTTGGCACGTCT‑‑‑‑GG‑CCAGCCTGGAGGTTAGCGAAACCTCCATCGATCTGGTCAAGCAGCAGGTCACCGTGGGCAAGGTTCGCAGCGAAAAGCTCg                               >  6:312304/1‑148 (MQ=255)                                                                                                                    aCGTCTCCGTGCGCATCGCGCCATTCGCCATCAAGGCGCCGGATGGCCGACCGTTGGCACGTCT‑‑‑‑GG‑CCAGCCTGGAGGTTAGCGAAACCTCCATCGATCTGGTCAAGCAGCAGGTCACCGTGGGCAAGGTTCGCAGCGAAAAGCTCGa                              <  2:183111/148‑1 (MQ=255)                                                                                                                     cGTCTCCGTGCGCATCGCGCCATTCGCCATCAAGGCGCCGGATGGCCGACCGTTGGCACGTCT‑‑‑‑GG‑CCAGCCTGGAGGTTAGCGAAACCTCCATCGATCTGGTCAAGCAGCAGGTCACCGTGGGCAAGGTTCGCAGCGAAAAGCt                                 >  7:191847/1‑144 (MQ=255)                                                                                                                     cGTCTCCGTGCGCATCGCGCCATTCGCCATCAAGGCGCCGGATGGCCGACCGTTGGCACGTCT‑‑‑‑GG‑CCAGCCTGGAGGTTAGCGAAACCTCCATCGATCTGGTCAAGCAGCAGGTCACCGTGGGCAAGGTTCGCAGCGAAAAGCt                                 <  8:191847/144‑1 (MQ=255)                                                                                                                           cGTGCGCATCGCGCCATTCGCCATCAAGGCGCCGGATGGCCGCCCGTTGGCACGTCT‑‑‑‑GG‑CCAGCCTGGAGGTTAGCGAAACCTCCATCGATCTGGTCAAGCAGCAGGTCACCGTGGGCAAGGTTCGCAGCGAAAAGCTCGAAACCTggg                      >  6:193827/1‑149 (MQ=255)                                                                                                                           cGTGCGCATCGCGCCATTCGCCATCAAGGCGCCGGATGGCCGACCGTTGGCACGTCT‑‑‑‑GG‑CCAGCCTGGAGGTTAGCGCAACCTCCATCGATCTGGTCAAGCAGCAGGTCACCGTGGGCAAGGTTCGCAGCGAAAAGCTCGAAACCTggg                      >  7:160793/1‑149 (MQ=255)                                                                                                                           cGTGCGCATCGCGCCATTCGCCATCAAGGCGCCGGATGGCCGACCGTTGGCACGTCT‑‑‑‑GG‑CCAGCCTGGAGGTTAGCGAAACCTCCATCGATCTGGTCAAGCAGCAGGTCACCGTGGGCAAGGTTCGCa                                           <  2:330282/128‑1 (MQ=255)                                                                                                                           cGTGCGCATCGCGCCATTCGCCATCAAGGCGCCGGATGGCCGACCGTTGGCACGTCT‑‑‑‑GG‑CCAGCCTGGAGGTTAGCGAAACCTCCATCGATCTGGTCAAGCAGCAGGTCACCGTGGGCAAGGTTCGCa                                           >  1:330282/1‑128 (MQ=255)                                                                                                                           cGTGCGCATCGCGCCATTCGCCATCAAGGCGCCGGATGGCCGACCGTTGGCACGTCT‑‑‑‑GG‑CCAGCCTGGAGGTTAGCGAAACCTCCATCGATCTGGTCAAGCAGCAGGTCACCGTGGGCAAGGTTCGCAGCGAAAAGCTCGAAACCTggg                      >  5:182342/1‑149 (MQ=255)                                                                                                                                  aTCGCGCCATTCGCCATCAAGGCGCCGGATGGCCGACCGTTGGCACGTCT‑‑‑‑GG‑CCAGCCTGGAGGTTAGCGAAACCTCCATCGATCTGGTCAAGCAGCAGGTCACCGTGGGCAAGGTTCGCAGCGAAAAGCTCGAAACCTGGGCGGCGCt               <  3:85925/149‑1 (MQ=255)                                                                                                                                       gcCATTCGCCATCAAGGCGCCGGATGGCCGACCGTTGGCACGTCT‑‑‑‑GG‑CCAGCCTGGAGGTTAGCGAAACCTCCATCGATCTGGTCAAGCAGCAGGTCACCGTGGGCAAGGTTCGCAGCGAAAAGCTCGAAACCTGGGCGGCGCTGGaa           >  1:157296/1‑148 (MQ=255)                                                                                                                                        ccATTCGCCATCAAGGCGCCGGATGGCCGACCGTTGGCACGTCT‑‑‑‑GG‑CCAGCCTGGAGGTTAGCGAAACCTCCATCGATCTGGTCAAGCAGCAGGTCACCGTGGGCAAGGTTCGCAGCGAAAAGCTCGAAACCTGGGCGGCGCTGGaaa          >  5:371314/1‑148 (MQ=255)                                                                                                                                        ccATTCGCCATCAAGGCGCCGGATGGCCGACCGTTGGCACGTCT‑‑‑‑GG‑CCAGCCTGGAGGTTAGCGAAACCTCCATCGATCTGGTCAAGCAGCAGGTCACCGTGGGCAAGGTTCGCAGCGAAAAGCTCGAAACCTGGGCGGCGCTGGaaa          >  4:308654/1‑148 (MQ=255)                                                                                                                                         cATTCGCCATCAAGGCGCCGGATGGCCGACCGTTGGCACGTCT‑‑‑‑GG‑CCAGCCTGGAGGTTAGCGAAACCTCCATCGATCTGGTCAAGCAGCAGGTCACCGTGGGCAAGGTTCGCAGCGAAAAGCTCGAAACCTGGGCGGCGCTGGaaaaa        <  2:120222/149‑1 (MQ=255)                                                                                                                                              gCCATCAAGGCGCCGGATGGCCGACCGTTGGCACGTCT‑‑‑‑GG‑CCAGCCTGGAGGTTAGCGAAACCTCCATCGATCTGGTCAAGCAGCAGGTCACCGTGGGCAAGGTTCGCAGCGAAAAGCTCGAAACCTGGGCGGCGCTGGAAAAAGATgg   >  6:200091/1‑149 (MQ=255)                                                                                                                                               ccATCAAGGCGCCGGATGGCCGACCGTTGGCACGTCT‑‑‑‑GG‑CCAGCCTGGAGGTTAGCGAAACCTCCATCGATCTGGTCAAGCAGCAGGTCACCGTGGGCAAGGTTCGCAGCGAAAAGCTCGAAACCTGGGCGGCGCTGGAAAAAGATGGc  <  6:9974/149‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                     CTGTTCTGGCCCTACGTGCGCGATGCCGTGCCACTGGTGCTGGAAGACGGCGTGGTAAGCCTCGATACGCATTACAAGCTCAACCTCGCCAAACAGACCGAGCTGCTGCTGGATAACGTCTCCGTGCGCATCGCGCCATTCGCCATCAAGGCGCCGGATGGCCGACCGTTGGCACGTCT‑‑‑‑GG‑CCAGCCTGGAGGTTAGCGAAACCTCCATCGATCTGGTCAAGCAGCAGGTCACCGTGGGCAAGGTTCGCAGCGAAAAGCTCGAAACCTGGGCGGCGCTGGAAAAAGATGGC  >  NC_002947/5348205‑5348495

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑