| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,360,178 | A→C | 8.3% | L31R (CTG→CGG) ‡ | nusA ← | transcription termination/antitermination factor L |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,360,178 | 0 | A | C | 8.3% | 180.2 / 7.8 | 60 | L31R (CTG→CGG) ‡ | nusA | transcription termination/antitermination factor L |
| Reads supporting (aligned to +/- strand): ref base A (29/26); new base C (3/2); total (32/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.52e-01 | |||||||||||
TCCAGCCAGGTCTCGATCGCCGGATCATCAAGATCGGCTTCGTCGACCACGGTCCAGCGACGGAAAGTCTCGT‑AGCTACCGGTGTGGCGGTTGATTTCCACACGTAGGTCGACTTCGTCTTCAAAACGTTTTTTGGTTGCAGTGGCCAGGGCCACTTCCAGCGCTTCGAAAATGACGCCGGGCGGTACACCTTTTTCGTTGGATACCGATTCAACAACCAGCAGTACTTCTTTGCTCATCGTACGCCTCGCCTTGCGCAAGCCATTGGGCCCGGATAGTCCGCCGGGCCCGG > NC_002947/5360031‑5360322 | tCCAGCCAGGTCTCGATCGCCGGATCATCAAGATCGGCTTCGTCGACCACGGTCCAGCGACGGAAAGTCTCGT‑AGCTACCGGTGTGGCGGTTGATTTCCACACGTAGGTCGACTTCGTCTTCAAAACGTTTTTTGGTTGCAGTGGCCAg > 4:201122/1‑149 (MQ=255) cagccagGTCTCGATCGCCGGATCATCAAGATCGGCTTCGTCGACCACGGTCCAGCGACGGAAAGTCTCGT‑AGCTACCGGTGTGGCGGTTGATTTCCACACGTAGGTCGACTTCGTCTTCAAAACGTTTTTTGGTTGCAGTGGCCAggg < 3:186623/149‑1 (MQ=255) cagccagGTCTCGATCGCCGGATCATCAAGATCGGCTTCGTCGACCACGGTCCAGCGACGGAAAGTCTCGT‑AGCTACCGGTGTGGCGGTTGATTTCCACACGTAGGTCGACTTCGTCTTCAAAACGTTTTTTGGTTGCAGTGGCCAggg > 4:326949/1‑149 (MQ=255) cagccagGTCTCGATCGCCGGATCATCAAGATCGGCTTCGTCGACCACGGTCCAGCGACGGAAAGTCTCGT‑AGCTACCGGTGTGGCGGTTGATTTCCACACGTAGGTCGACTTCGTCTTCAAAACGTTTTTTGGTTGCAGTGGCCAgg < 4:58664/148‑1 (MQ=255) agccagGTCTCGATCGCAGGATCATCAAGATCGGCTTCGTCGACCACGGTCCAGCGACGGAAAGTCTCGT‑AGCTACCGGTGTGGCGGTTGATTTCCACACGTAGGTCGACTTCGTCTTCAAAACGTTTTTTGGTTGCAGTGGCCAGGGc < 7:84135/149‑1 (MQ=255) ccagGTCTCGATCGCCGGATCATCAAGATCGGCTTCGTCGACCACGGTCCAGCGACGGAAAGTCTCGT‑AGCTACCGGTGTGGCGGTTGATTTCCACACGTAGGTCGACTTCGTCTTCAAAACGTTTTTTGGTTGCAGTGGCCAGGGCCa > 6:328541/1‑149 (MQ=255) gTCTCGATCGCCGGATCATCAAGATCGGCTTCGTCGACCACGGTCCAGCGACGGAAAGTCTCGT‑AGCTACGGGTGTGGCGGTTGATTTCCACACGTAGGTCGACTTCGTCTTCAAAACGTTTTTTGGTTGCAGTGGCCAGGGCCACTTc < 6:208463/149‑1 (MQ=255) tcGATCGCCGGATCATCAAGATCGGCTTCGTCGACCACGGTCCAGCGACGGAAAGTCTCGTAAGATACCGGTGTGGCGGTTGATTTCCAAACGTAGGTCGACTTCGTCTTCAAAACGTTTTTTGGTTGCAGTGGCCAGGGCCACTTc > 1:107784/1‑147 (MQ=255) aTCGCCGGATCATCAAGATCGGCTTCGTCGACCACGGTCCAGCGACGGAAAGTCTCGT‑AGCTACCGGTGTGGCGGTTGATTTCCACACGTAGGTCGGCTTCGTCTTCAAAACGTTTTTTGGTTGCAGTGGCCAGGGCCACTTCCAgcgc < 8:199712/149‑1 (MQ=255) gCCGGATCATCAAGATCGGCTTCGTCGACCACGGTCCAGCGACGGAAAGTCTCGT‑AGCTACCGGTGTGGCGGTTGATTTCCACACGTAGGTCGACTTCGTCTTCAAAACGTTTTTTGGTTGCAGTGGCCAGGGCCACTTCCAGCGCTTc > 1:80081/1‑149 (MQ=255) gATCATCAAGATCGGCTTCGTCGACCACGGTCCAGCGACGGAAAGTCTCGT‑AGCTACCGGTGTGGCGGTTGATTTCCACACGTAGGTCGACTTCGTCTTCAAAACGTTTTTTGGTTGCAGTGGCCAGGGCCACTTCCAGCGCTTCGaaa < 3:201122/149‑1 (MQ=255) tcaAGATCGGCTTCGTCGACCACGGTCCAGCGACGGAAAGTCTCGT‑AGCTACCGGTGTGGCGGTTGATTTCCACACGTAGGTCGACTTCGTCTTCAAAACGTTTTTTGGTTGCAGTGGCCAGGGCCACTTCCAGCGCTTCGAAAATGAc > 5:124405/1‑149 (MQ=255) caAGATCGGCTTCGTCGACCACGGTCCAGCGACGGAAAGTCTCGT‑AGCTACCGGTGTGGCGGTTGATTTCCACACGTAGGTCGACTTCGTCTTCAAAACGTTTTTTGGTTGCAGTGGCCAGGGCCAc > 1:172181/1‑127 (MQ=255) caAGATCGGCTTCGTCGACCACGGTCCAGCGACGGAAAGTCTCGT‑AGCTACCGGTGTGGCGGTTGATTTCCACACGTAGGTCGACTTCGTCTTCAAAACGTTTTTTGGTTGCAGTGGCCAGGGCCAc < 2:172181/127‑1 (MQ=255) aTCGGCTTCGTCGACCACGGTCCAGCGACGGAAAGTCTCGT‑AGCTACCGGTGTGGCGGTTGATTTCCACACGTAGGTCGACTTCGTCTTCAAAACGTTTTTTGGTTGCAGTGGCCAGGGCCACTTCCAGCGCTTCGAAAATGACGCCgg < 8:66623/149‑1 (MQ=255) gCTTCGTCGACCACGGTCCAGCGACGGAAAGTCTCGT‑AGCTACCGGTGTGGCGGTTGATTTCCACACGTAGGTCGACTTCGTCTTCAAAACGTTTTTTGGTTGCAGTGGCCAGTGCCACTTCCAGCGCTTCGAAAATGACGCCGGGCgg > 3:344896/1‑149 (MQ=255) cTTCGTCGACCACGGTCCAGCGACGGAAAGTCTCGT‑AGCTACCGGTGTGGCGGTTGATTTCCACACGTAGGTCGACTTCGTCTTCAAAACGTTTTTTGGTTGCAGTGGCCAGTGCCACTTCCAGCGCTTCGAAAATGACGCCGGGCGGt < 4:344896/149‑1 (MQ=255) ttCGTCGACCACGGTCCAGCGACGGAAAGTCTCGT‑AGCTACCGGTGTGGCGGTTGATTTCCACACGTAGGTCGACTTCGTCTTCAAAACGTTTTTTGGTTGCAGTGGCCAGGGCCACTTCCAGCGCTTCGAAAATGACGCCGGGCGGTa > 6:322029/1‑149 (MQ=255) tcgACCACGGTCCAGCGACGGAAAGTCTCGT‑AGCTACCGGTGTGGCGGTTGATTTCCACACGTAGGTCGACTTCGTCTTCAAAACGTTTTTTGGTTGCAGTGGCCAGGGCCACTTCCAGCGCTTCGAAAATGACGCCGGGCGGTACAcc > 2:249193/1‑149 (MQ=255) cgACCACGGTCCAGCGACGGAAAGTCTCGT‑AGCTACCGGTGTGGCGGTTGATTTCCACACGTAGGTCGACTTCGTCTTCAAAACGTTTTTTGGTTGCAGTGGCCAGGGCCACTTCCAGCGCTTCGAAAATGACGCCGGGCGGTACAcc < 1:249193/148‑1 (MQ=255) gtacAGCGACGGAAAGGCTCGT‑AGCTACCGGTGTGGCGGTTGATTTCCACACGTAGGTCGACTTCGTCTTCAAAACGTTTTTTGGTTGCAGTGGCCAGGGCCACTTCCAGCGCTTCGAAAATGACGCCGGGCGGTACACCTTTTTCGtt < 8:311642/146‑1 (MQ=255) tCCAGCGACGGAAAGTCTCGT‑AGCTACCGGTGTGGCGGTTGATTTCCACACGTAGGTCGACTTCGTCTTCAAAACGTTTTTTGGTTGCAGTGGCCAGGGCCACTTCCAGCGCTTCGAAAATGACGCCGGGCGGTACACCTTTTTCGTTg < 2:80081/149‑1 (MQ=255) tCCAGCGACGGAAAGTCTCGT‑AGCTACCGGTGTGGCGGTTGATTTCCACACGTAGGTCGACTTCGTCTTCAAAACGTTTTTTGGTTGCAGTGGCCAGGGCCACTTCCAGCGCTTCGAAAATGACGCCGGGCGGTACACCTTTTTCGTTg > 4:317086/1‑149 (MQ=255) cAGCGACGGAAAGTCTCGT‑AGCTACCGGTGTGGCGGTTGATTTCCACACGTAGGTCGACTTCGTCTTCAAAACGTTTTTTGGTTGCAGTGGCCAGGGCCACTTCCAGCGCTTCGAAAATGACGCCGGGCGGTACACCTTTTTCGTTGGa < 5:328541/149‑1 (MQ=255) aGCGACGGAAAGTCTCGT‑AGCTACCGGTGTGGCGGTTGATTTCCACACGTAGGTCGACTTCGTCTTCAAAACGTTTTTTGGTTGCAGTGGCCAGGGCCACTTCCAGCGCTTCGAAAATGACGCCGGGCGGTACACCTTTTTCGTTGGa > 4:52140/1‑148 (MQ=255) cGACGGAAAGTCTCGT‑AGCTACCGGTGTGGCGGTTGATTTCCACACGTAGGTCGACTTCGTCTTCAAAACGTTTTTTGGTTGCAGTGGCCAGGGCCACTTCCAGCGCTTCGAAAATGACGCCGGGCGGTACACCTTTTTCGTTGGATAc < 2:107784/149‑1 (MQ=255) gAAAGTCTCGT‑AGCTACCGGTGTGGCGGTTGATTTCCACACGTAGGTCGACTTCGTCTTCAAAACGTTTTTTGGTTGCAGTGGCCAGGGCCACTTCCAGCGCTTCGAAAATGACGCCGGGCGGTACAACTTTTTCGTTGTATACAGAt > 3:24871/1‑148 (MQ=255) aaaGTCTCGT‑AGCTACCGGTGTGGCGGTTGATTTCCACACGTAGGTCGACTTCGTCTTCAAAACGTTTTTTGGTTGCAGTGGCCAGGGCCACTTCCAGCGCTTCGAAAATGACGCCgg > 4:245475/1‑118 (MQ=255) aaaGTCTCGT‑AGCTACCGGTGTGGCGGTTGATTTCCACACGTAGGTCGACTTCGTCTTCAAAACGTTTTTTGGTTGCAGTGGCCAGGGCCACTTCCAGCGCTTCGAAAATGACGCCgg < 3:245475/118‑1 (MQ=255) aaaGTCTCGT‑AGCTACCGGTGTGGCGGTTGATTTCCACACGTAGGTCGACTTCGTCTTCAAAACGTTTTTTGGTTGCAGTGGCCAGGGCCACTTCCAGCGCTTCGAAAATGACGCCGGGCGGTACACCTTTTTCGTTGGATACCGAtt < 8:317226/148‑1 (MQ=255) aaaGTCTCGT‑AGCTACCGGTGTGGCGGTTGATTTCCACACGTAGGTCGACTTCGTCTTCAAAACGTTTTTTGGTTGCAGTGGCCAGGGCCACTTCCAGCGCTTCGAAAATGACGCCGGGCGGTACACCTTTTTCGTTGGATACCGAtt > 7:317226/1‑148 (MQ=255) gTCTCGT‑AGCTACCGGTGTGGCGGTTGATTTCCACACGTAGGTCGACTTCGTCTTCAAAACGCTTTTTGGTTGCAGTGGCCAGGGCCACTTCCAGCGCTTCGAAAATGACGCCGGGCGGTACACCTTTTTCGTTGGATACCGATTcaac < 3:317086/149‑1 (MQ=255) tcGT‑AGCTACCGGTGTGGCGGTTGATTTCCACACGTAGGTCGACTTCGTCTTCAAAACGTTTTTTGGTTGCAGTGGCCAGGGCCACTTCCAGCGCTTCGAAAATGACGCCGGGCGGTACACCTTTTTCGTTGGATACCGATTCaacaac > 7:189906/1‑149 (MQ=255) t‑AGCTACCGGTGTGGCGGTTGATTTCCACACGTAGGTCGACTTCGTCTTCAAAACGTTTTTTGGTTGCAGTGGCCAGGGCCACTTCCAGCGCTTCGAAAATGACGCCGGGCGGTACACCTTTTTCGTTGGATACCGATTCAACAACcag < 5:322029/149‑1 (MQ=255) gCTACCGGTGTGGCGGTTGATTTCCACACGTAGGTCGACTTCGTCTTCAAAACGTTTTTTGGTTGCAGTGGCCAGGGCCACTTCCAGCGCTTCGAAAATGACGCCGGGCGGTACACCTTTTTCGTTGGATACCGATTCAACAACcagca < 3:311375/149‑1 (MQ=255) aCCGGTGTGGCGGTTGATTTCCACACGTAGGTCGACTTCGTCTTCAAAACGTTTTTTGGTTGCAGTGGCCAGGGCCACTTCCAGCGCTTCGAAAATGACGCCGGGCGGTACACCTTTTTCGTTGGATACCGATTCAACAACCAGCAGTa > 8:168637/1‑149 (MQ=255) tgGCGGTTGATTTCCACACGTAGGTCGACTTCGTCTTCAAAACGTTTTTTGGTTGCAGTGGCCAGGGCCACTTCCAGCGCTTCGAAAATGACGCCGGGCGGTACACCTTTTTCGTTGGATACCGATTCAACAACCAGCAGTACTTCttt < 3:326949/149‑1 (MQ=255) tgGCGGTTGATTTCCACACGTAGGTCGACTTCGTCTTCAAAACGTTTTTTGGTTGCAGTGGCCAGGGCCACTTCCAGCGCTTCGAAAATGACGCCGGGCGGTACACCTTTTTCGTTGGATACCGATTCAACAACCAGCAGTACTTCttt > 6:94888/1‑149 (MQ=255) cacaCGTAGGTCGACTTCGTCTTCAAAACGTTTTTTGGTTGCAGTGGCCAGGGCCACTTCCAGCGCTTCGAAAATGACGCCGGGCGGTACACCTTTTTCGTTGGATACCGATTCAACAACCAGCAGTACTTCTTTGCTCATCGTAcgc > 7:283911/1‑148 (MQ=255) gTAGGTCGACTTCGTCTTCAAAACGTTTTTTGGTTGCAGTGGCCAGGGCCACTTCCAGCGCTTCGAAAATGACGCCGGGCGGTACACCTTTTTCGTTGGATACCGATTCAACAACCAGCAGTACTTCTTTGCTCATCGTAcgcctcgcc < 5:94888/149‑1 (MQ=255) gTAGGTCGACTTCGTCTTCAAAACGTTTTTTGGTTGCAGTGGCCAGGGCCACTTCCAGCGCTTCGAAAATGACGCCGGGCGGTACACCTTTTTCGTTGGATACCGATTCAACAACCAGCAGTACTTCTTTGCTCATCGTAcgcctcgcc > 4:313219/1‑149 (MQ=255) cGTCTTCAAAACGTTTTTTGGTTGCAGTGGCCAGGGCCACTTCCAGCGCTTCGAAAATGACGCCGGGCGGTACACCTTTTTCGTTGGATACCGATTCaacaac > 6:352752/1‑103 (MQ=255) cGTCTTCAAAACGTTTTTTGGTTGCAGTGGCCAGGGCCACTTCCAGCGCTTCGAAAATGACGCCGGGCGGTACACCTTTTTCGTTGGATACCGATTCaacaac < 5:352752/103‑1 (MQ=255) tCTTCAAAACGTTTTTTGGTTGCAGTGGCCAGGGCCACTTCCAGCGCTTCGAAAATGACGCCGGGCGGTACACCTTTTTCGTTGGATACCGATTCAACAACCAGCAGTACTTCTTTGCTCATCGTACGCCTCGCCTTGCGCAAGCCAtt > 7:135545/1‑149 (MQ=255) ttCAAAACGTTTTTTGGTTGCAGTGGCCAGGGCCACTTCCAGCGCTTCGAAAATGACGCCGGGCGGTACACCTTTTTCGTTg < 3:192035/82‑1 (MQ=255) aaaCGTTTTTTGGTTGCAGTGGCCAGGGCCACTTCCAGCGCTTCGAAAATGACGCCGGGCGGTACACCTTTTTCGTTGGATACCGATTCAACAACCAGCAGTACTTCTTTGCTCATCGTACGCCTCGCCTTGCGCAAGCCATTGGGccc < 6:124405/149‑1 (MQ=255) ttttttGGTTGCAGTGGCCAGGGCCACTTCCAGCGCTTCGAAAATGACGCCGGGCGGTACACCTTTTTCGTTGGATACCGATTCAACAACCAGCAGTACTTCTTTGCTCATCGTACGCCTCGCCTTGCGCAAGCCATTGGGCCCGGATa > 4:90923/1‑149 (MQ=255) gTTGCAGTGGCCAGGGCCACTTCCAGCGCTTCGAAAATGACGCCGGGCGGTACACCTTTTTCGTTGGATACCGATTCAACAACCAGCAGTACTTCTTTGCTCATCGTACGCCTCGCCTTGCGCAAGCCATTGGGCCCGGATAGTccgcc > 6:352986/1‑149 (MQ=255) gaaGTGGCCCTGGCCACTTCCAGCGCTTCGAAAATGACGCCGGGCGGTACACCTTTTTCGTTGGATACCGATTCAACAACCAGCAGTACTTCTTTGCTCATCGTACGCCTCGCCTTGCGCAAGCCATTGGGCCCgg < 4:289933/134‑1 (MQ=255) gaaGTGGCCCTGGCCACTTCCAGCGCTTCGAAAATGACGCCGGGCGGTACACCTTTTTCGTTGGATACCGATTCAACAACCAGCAGTACTTCTTTGCTCATCGTACGCCTCGCCTTGCGCAAGCCATTGGGCCCgg > 3:289933/3‑136 (MQ=255) gaaGTGGCCCTGGCCACTTCCAGCGCTTCGAAAATGACGCCGGGCGGTACACCTTTTTCGTTGGATACCGATTCAACAACCAGCAGTACTTCTTTGCTCATCGTACGCCTCGCCTTGCGCAAGCCATTGGGCCCGGATAGTCCGCCggg > 8:296589/3‑149 (MQ=255) gCAGTGGCCAGGGCCACTTCCAGCGCTTCGAAAATGACGCCGGGCGGTACACCTTTTTCGTTGGATACCGATTCAACAACCAGCAGTACTTCTTTGCTCATCGTACGCCTCGCCTTGCGCAAGCCATTGGGCCCGGATAGTCCGCCggg > 7:329787/1‑149 (MQ=255) cAGTGGCCAGGGCCACTTCCAGCGCTTCGAAAATGACGCCGGGCGGTACACCTTTTTCGTTGGATACCGATTCAACAACCAGCAGTACTTCTTTGCTCATCGTACGCCTCGCCTTGCGCAAGCCATTGGGCCCGGATAGTCCGCCGGGc > 1:229367/1‑149 (MQ=255) aGTGGCCAGGGCCACTTCCAGCGCTTCGAAAATGACGCCGGGCGGTACACCTTTTTCGTTGGATACCGATTCAACAACCAGCAGTACTTCTTTGCTCATCGTACGCCTCGCCTTGCGCAAGCCATTGGGCCCGGATAGTCCGCCGGGcc > 3:219388/1‑149 (MQ=255) gTGGCCCTGGCCACTTCCAGCGCTTCGAAAATGACGCCGGGCGGTACACCTTTTTCGTTGGATACCGATTCAACAACCAGCAGTACTTCTTTGCTCATCGTACGCCTCGCCTTgc > 6:61604/1‑115 (MQ=255) gTGGCCCTGGCCACTTCCAGCGCTTCGAAAATGACGCCGGGCGGTACACCTTTTTCGTTGGATACCGATTCAACAACCAGCAGTACTTCTTTGCTCATCGTACGCCTCGCCTTgc < 5:61604/115‑1 (MQ=255) gTGGCCAGGGCCACTTCCAGCGCTTCGAAAATGACGCCGGGCGGTACACCTTTTTCGTTGGATACCGATTCAACAACCAGCAGTACTTCTTTGCTCATCGTACGCCTCGCCTTGCGCAAGCCATTGGGCCCGGATAGTCCGCCGGGccc > 4:309106/1‑149 (MQ=255) gTGGCCAGGGCCACTTCCAGCGCTTCGAAAATGACGCCGGGCGGTACACCTTTTTCGTTGGATACCGATTCAACAACCAGCAGTACTTCTTTGCTCATCGTACGCCTCGCCTTGCGCAAGCCATTGGGCCCGGATAGTCCGCCGGGccc < 3:52140/149‑1 (MQ=255) tGGCCAGGGCCACTTCCAGCGCTTCGAAAATGACGCCGGGCGGTACACCTTTTTCGTTGGATACCGATTCAACAACCAGCAGTACTTCTTTGCTCATCGTACGCCTCGCCTTGCGCAAGCCATTGGGCCCGGATAGTCCGCCGGGCCCg < 4:219388/149‑1 (MQ=255) gCCAGGGCCACTTCCAGCGCTTCGAAAATGACGCCGGGCGGTACACCTTTTTCGTTGGATACCGATTCAACAACCAGCAGTACTTCTTTGCTCATCGTACGCCTCGCCTTGCGCAAGCCATTGGGCCCGGATAGTCCGCCGGGCCCgg > 8:34092/1‑148 (MQ=255) | TCCAGCCAGGTCTCGATCGCCGGATCATCAAGATCGGCTTCGTCGACCACGGTCCAGCGACGGAAAGTCTCGT‑AGCTACCGGTGTGGCGGTTGATTTCCACACGTAGGTCGACTTCGTCTTCAAAACGTTTTTTGGTTGCAGTGGCCAGGGCCACTTCCAGCGCTTCGAAAATGACGCCGGGCGGTACACCTTTTTCGTTGGATACCGATTCAACAACCAGCAGTACTTCTTTGCTCATCGTACGCCTCGCCTTGCGCAAGCCATTGGGCCCGGATAGTCCGCCGGGCCCGG > NC_002947/5360031‑5360322 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |