Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,363,327 C→T 7.0% V147I (GTT→ATT)  glmM ← phosphoglucosamine mutase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,363,3270CT 7.0% 167.3 / 2.1 57V147I (GTT→ATT) glmMphosphoglucosamine mutase
Reads supporting (aligned to +/- strand):  ref base C (27/26);  new base T (2/2);  total (29/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.65e-01

ACGGAATACGCTGGGCGCAACCTTGTAGGTAGCACCATGGGCGCAGTCGACCACCAGCTTGAGGCCTTCGAAGCTGGTGCTGCTCGGCACACTGCTCTTGCAGAATTCGATGTAGCGGCCGGCGGCATCGTTGATACGCGAAACCTTGCCCAGCTTGCCCGACTCGACAACGGTCATCGGTTGGTCGAGCAGCTCTTCGATCATCAGCTCGACCTCGTCCGGCAGCTTGGTGCCCTGGCCCGAGAAGAACTTGATGCCGTTGTCATCGTGCGGGTTGTGCGAGGCACTGAT  >  NC_002947/5363184‑5363474
                                                                                                                                               |                                                                                                                                                   
aCGGAATACGCTGGGCGCAACCTTGTAGGTAGCACCATGGGCGCAGTCGACCACCAGCTTGAGGCCTTCGAAGCTGGTGCTGCTCGGCACACTGCTCTTGCAGAATTCGATGTAGCGGCCGGCGGCATCGTTGATACGCGAAACCTTg                                                                                                                                                 <  4:181698/148‑1 (MQ=255)
      tACGCTGGGCGCAACCTTGTAGGTAGCACCATGGGCGCAGTCGACCACCAGCTTGAGGCCTTCGAAGCTGGTGCTGCTCGGCACACTGCTCTTGCAGAATTCGATGTAGCGGCCGGCGGCATCGTTGATACGCGAAACCTTGCCCAGCt                                                                                                                                          <  6:349628/149‑1 (MQ=255)
        cGCTGGGCGCAACCTTGTAGGTAGCACCATGGGCGCAGTCGACCACCAGCTTGAGGCCTTCGAAGCTGGTGCTGCTCGGCACACTGCTCTTGCAGAATTCGATGTAGCGGCCGGCGGCATCGTTGATACGCGAAACCTTGCCCAGCTTg                                                                                                                                        >  6:145260/1‑149 (MQ=255)
         gCTGGGCGCAACCTTGTAGGTAGCACCATGGGCGCAGTCGACCACCAGCTTGAGGCCTTCGAAGCTGGTGCTGCTCGGCACACTGCTCTTGCAGAATTCGATGTAGCGGCCGGCGGCATCGTTGATACGCGAAACCTTGCCCAGCTTGc                                                                                                                                       >  7:44503/1‑149 (MQ=255)
             ggCGCAACCTTGTAGGTAGCACCATGGGCGCAGTCGACCACCAGCTTGAGGCCTTCGAAGCTGGTGCTGCTCGGCACACTGCTCTTGCAGAATTCGATGTAGCGGCCGGCGGCATCGTTGATACGCGAAACCTTGCCCAGCTTGccc                                                                                                                                     <  1:99423/147‑1 (MQ=255)
              gcgcAACCTTGTAGGTAGCACCATGGGCGCAGTCGACCACCAGCTTGAGGCCTTCGAAGCTGGTGCTGCTCGGCACACTGCTCTTGCAGAATTCGATGTAGCGGCCGGCGGCATCGTTGATACGCGAAACCTTGCCCAg                                                                                                                                            <  5:330695/139‑1 (MQ=255)
              gcgcAACCTTGTAGGTAGCACCATGGGCGCAGTCGACCACCAGCTTGAGGCCTTCGAAGCTGGTGCTGCTCGGCACACTGCTCTTGCAGAATTCGATGTAGCGGCCGGCGGCATCGTTGATACGCGAAACCTTGCCCAg                                                                                                                                            >  6:330695/1‑139 (MQ=255)
                      ttGTAGGTAGCACCATGGGCGCAGTCGACCACCAGCTTGAGGCCTTCGAAGCTGGTGCTGCTCGGCACACTGCTCTTGCAGAATTCGATGTAGCGGCCGGCGGCATCGTTGATACGCGAAACCTTGCCCAGCTTGCCCGACTCGACAAc                                                                                                                          >  8:307398/1‑149 (MQ=255)
                                cACCATGGGCGCAGTCGACCACCAGCTTGAGGCCTTCGAAGCTGGTGCTGCTCGGCACACTGCTCTTGCAGAATTCGATGTAGCGGCCGGCGGCATCGTTGATACGCGAAACCTTGCCCAGCTTGCCCGACTCGACAACGGTCATCGGt                                                                                                                >  1:53250/1‑149 (MQ=255)
                                cACCATGGGCGCAGTCGACCACCAGCTTGAGGCCTTCGAAGCTGGTGCTGCTCGGCACACTGCTCTTGCAGAATTCGATGTAGCGGCCGGCGGCATCGTTGATACGCGAAACCTTGCCCAGCTTGCCCGACTCGACAACGGTCATCGGt                                                                                                                >  1:58468/1‑149 (MQ=255)
                                 tcgaTGGGATCAGGCGAACACCAGATTGAGGGCTTCGAAGATGGTGCTGCGAGGCTCACTGCTCTTGCAGAATTCGATGTAGCGGCCGTCGGCAGCTTTGATACGCGAAACCTTGGCCAGCTTGCCCGACTGGAGAACGGTCATCTGt                                                                                                                <  8:60039/145‑1 (MQ=255)
                                 aCCATGGGCGCAGTCGACCACCAGCTTGAGGCCTTCGAAGCTGGTGCTGCTCGGCACACTGCTCTTGCAGAATTCGATGTAGCGGCCGGCGGCATCGTTGATACGCGAAACCTTGCCCAGCTTGCCCGACTCGACAACGGTCATCGGtt                                                                                                               <  3:194051/149‑1 (MQ=255)
                                 aCCATGGGCGCAGTCGACCACCAGCTTGAGGCCTTCGAAGCTGGTGCTGCTCGGCACACTGCTCTTGCAGAATTCGATGTAGCGGCCGGCGGCATCGTTGATACGCGAAACCTTGCCCAGCTTGCCCGACTCGACAACGGTCATCGGt                                                                                                                >  7:60039/1‑148 (MQ=255)
                                   cATGGGCGCAGTCGACCACCAGCTTGAGGCCTTCGAAGCTGGTGCTGCTCGGCACACTGCTCTTGCAGAATTCGATGTAGCGGCCGGCGGCATCGTTGATACGCGAAACCTTGCCCAGCTTGCCCGACTCGACAACGGTCATCGGTTg                                                                                                              >  8:234770/1‑148 (MQ=255)
                                   cATGGGCGCAGTCGACCACCAGCTTGAGGCCTTCGAAGCTGGTGCTGCTCGGCACACTGCTCTTGCAGAATTCGATGTAGCGGCCGGCGGCATCGTTGATACGCGAAACCTTGCCCAGCTTGCCCGACTCGACAACGGTCATCGGTTg                                                                                                              <  7:234770/148‑1 (MQ=255)
                                    aTGGGCGCAGTCGACCACCAGCTTGAGGCCTTCGAAGCTGGTGCTGCTCGGCACACTGCTCTTGCAGAATTCGATGTAGCGGCCGGCGGCATCGTTGATACGCGAAACCTTGCCCATCTTGCCCGACTCGACAACGGTCATCGGTTGGt                                                                                                            <  4:328772/149‑1 (MQ=255)
                                        gcgcAGTCGACCACCAGCTTGAGGCCTTCGAAGCTGGTGCTGCTCGGCACACTGCTCTTGCAGAATTCGATGTAGCGGCCGGCGGCATCGTTGATACGCGAAACCTTGCCCAGCTTGCCCGACTCGACAACGGTCATCGGTTGGTCGag                                                                                                        <  3:340173/149‑1 (MQ=255)
                                         cgcAGTCGACCACCAGCTTGAGGCCTTCGAAGCTGGTGCTGCTCGGCACACTGCTCTTGCAGAATTCGATGTAGCGGCCGGCGGCATCGTTGATACGCGAAACCTTGCCCAGCTTGCCCGACTCGACAACGGTCATCGGTTGGTCGagc                                                                                                       <  5:145260/149‑1 (MQ=255)
                                                gACCACCAGCTTGAGGCCTTCGAAGCTGGTGCTGCTCGGCACACTGCTCTTGCAGAATTCGATGTAGCGGCCGGCGGCATCGTTGATACGCGAAACCTTGCCCAGCTTGCCCGACTCGACAACGGTCATCGGTTGGTCGAGCAGCTCtt                                                                                                <  7:307398/149‑1 (MQ=255)
                                                gACCACCAGCTTGAGGCCTTCGAAGCTGGTGCTGCTCGGCACACTGCTCTTGCAGAATTCGATGTAGCGGCCGGCGGCATCGTTGATACGCGAAACCTTGCCCAGCTTGCCCGACTCGACAACGGTCATCGGTTGGTCGAGCAGCTCtt                                                                                                >  4:97390/1‑149 (MQ=255)
                                                 accaccAGCTTGAGGCCTTCGAAGCTGGTGCTGCTCGGCACACTGCTCTTGCAGAATTCGATGTAGCGGCCGGCGGCATCGTTGATACGCGAAACCTTGCCCAGCTTGCCCGACTCGACAACGGTCATCGGTTGGTCGAGCAGCTCTTc                                                                                               <  2:53250/149‑1 (MQ=255)
                                                 accaccAGCTTGAGGCCTTCGAAGCTGGTGCTGCTCGGCACACTGCTCTTGCAGAATTCGATGTAGCGGCCGGCGGCATCGTTGATACGCGAAACCTTGCCCAGCTTGCCCGACTCGACAACGGTCATCGGTTGGTCGAGCAGCTCTTc                                                                                               <  2:58468/149‑1 (MQ=255)
                                                               gCCTTCGAAGCTGGTGCCGCTCGGCACACTGCTCTTGCAGAATTCGATGTAGCGGCCGGCGGCATCGTTGATACGCGAAACCTTGCCCAGCTTGCCCGACTCGACAACGGTCATCGGTTGGTCGAGCAGCTCTTCGATCATCAGCTCGa                                                                                 >  3:46544/1‑149 (MQ=255)
                                                                 cTTCGAAGCTGGTGCTGCTCGGCACACTGCTCTTGCAGAATTCGATGTAGCGGCCGGCGGCATCGTTGATACGCGAAACCTTGCCCAGCTTGCCCGACTCGACAACGGTCATCGGTTGGTCGAGCAGCTCTTCGATCATCAGCTCGAcc                                                                               >  4:230170/1‑149 (MQ=255)
                                                                  ttCGAAGCTGGTGCTGCTCGGCACACTGCTCTTGCAGAATTCGATGTAGCGGCCGGCGGCATCGTTGATACGCGAAATCTTGCCCAGCTTGCCCGACTCGACAACGGTCATCGGTTGGTCGAGCAGCTCTTCGATCATCAGCTCGACCt                                                                              >  5:135530/1‑149 (MQ=255)
                                                                  ttCGAAGCTGGTGCTGCTCGGCACACTGCTCTTGCAGAATTCGATGTAGCGGCCGGCGGCATCGTTGATACGCGAAATCTTGCCCAGCTTGCCCGACTCGACAACGGTCATCGGTTGGTCGAGCAGCTCTTCGATCATCAGCTCGACCt                                                                              >  5:141143/1‑149 (MQ=255)
                                                                  ttCGAAGCTGGTGATGCTCGGCACACTGCTCTTGCAGAATTCGATGTAGCGGCCGGCGGCATCGTTGATACGCGAAACCTAGCCCAGCTTGCCCGACTCGACAACGGTCATCGGTTGGTCGAGCAGATCATCGATCATCAGCTCGACCt                                                                              >  6:199967/1‑149 (MQ=255)
                                                                         cTGGTGCTGCTCGGCACACTGCTCTTGCAGAATTCGATGTAGCGGCCGGCGGCATCGTTGATACGCGAAACCTTGCCCAGCTTGCCCGACTCGACAACGGTCATCGGTTGGTCGAGCAGCTCTTCGATCATCAGCTCGACCTCGTCCgg                                                                       <  5:42512/149‑1 (MQ=255)
                                                                                          acTGCTCTTGCAGAATTCGATGTAGCGGCCGGCGGCATCGTTGATACGCGAAACCTTGCCCAGCTTGCCCGACt                                                                                                                                 >  7:185765/1‑74 (MQ=255)
                                                                                          acTGCTCTTGCAGAATTCGATGTAGCGGCCGGCGGCATCGTTGATACGCGAAACCTTGCCCAGCTTGCCCGACt                                                                                                                                 <  8:185765/74‑1 (MQ=255)
                                                                                          acTGCTCTTGCAGAATTCGATGTAGCGGCCGGCGGCATCGTTGATACGCGAAACCTTGCCCAGCTTGCCCGACTCGACAACGGTCATCGGTTGGTCGAGCAGCTCTTCGATCATCAGCTCGACCTCGTACGGCAGCTTGGTGCCCTGGc                                                      >  8:236948/1‑149 (MQ=255)
                                                                                              ctctTGCAGAATTCGATGTAGCGGCCGGCGGCATCGTTGATACGCGAAACCTTGCCCAGCTTGCCCGACTCGACAACGGTCATCGGTTGGTCGAGCAGCTCTTCGatcatc                                                                                        <  4:287707/111‑1 (MQ=255)
                                                                                              ctctTGCAGAATTCGATGTAGCGGCCGGCGGCATCGTTGATACGCGAAACCTTGCCCAGCTTGCCCGACTCGACAACGGTCATCGGTTGGTCGAGCAGCTCTTCGatcatc                                                                                        >  3:287707/1‑111 (MQ=255)
                                                                                                ctTGCAGAATTCGATGTAGCGGCCGGCGGCATCGTTGATACGCGAAACCTTGCCCAGCTTGCCCGACTCGACAACGGTCATCGGTTGGTCGAGCAGCTCTTCGATCATCAGCTCGACCTCGTCCGGCAGCTTGGTGCCCTGACCCgag                                                 >  5:211853/1‑148 (MQ=255)
                                                                                                   gCAGAATTCGATGTAGCGGCCGGCGGCATCGTTGATACGCGAAACCTTGCCCAGCTTGCCCGACTCGACAACGGTCATCGGTTGGTCGAGCAGCTCTTCGATCATCAGCTCGACCTCGTCCGGCAGCTTGGTGCCCTGGCCCGagaa                                               <  6:359878/147‑1 (MQ=255)
                                                                                                     aGAATTCGATGTAGCGGCCGGCGGCATCGTTGATACGCGAAACCTTGCCCAGCTTGCCCGACTCGACAACGGTCATCGGTTGGTCGAGCAGCTCTTCGATCATCAGCTCGACCTCGTCCGGCAGCTTGGTGCCCTGGCCCGAGAAGAAc                                           >  8:88272/1‑149 (MQ=255)
                                                                                                         ttCGATGTAGCGGCCGGCGGCATCGTTGATACGCGAAACCTTGCCCAGCTTGCCCGACTCGACAACGGTCATCGGTTGGTCGAGCAGCTCTTCGATCATCAGCTCGCCCTCGTCCGGCAGCTTGGTGCCCTGGCCCGAGAAGAACTTGa                                       >  7:246683/1‑149 (MQ=255)
                                                                                                            gATGTAGCGGCCGGCGGCATCGTTGATACGCGAAACCTTGCCCAGCTTGCCCGACTCGACAACGGTCATCGGTTGGTCGAGCAGCTCTTCGATCATCAGCTCGACCTCGTCCGGCAGCTTGGTGCCCTGGCCCGAGAAGAACTTGATGc                                    >  2:79754/1‑149 (MQ=255)
                                                                                                                tAGCGGCCGGCGGCATCGTTGATACGCGAAATCTTGCCCAGCTTGCCCGACTCGACAACGGTCATCGGTTGGTCGAGCAGCTCTTCGATCATCAGCTCGACCTCGTCCGGCAGCTTGGTGCCCTGGCCCGAGAAGAACTTGATGCCGtt                                <  6:141143/149‑1 (MQ=255)
                                                                                                                tAGCGGCCGGCGGCATCGTTGATACGCGAAATCTTGCCCAGCTTGCCCGACTCGACAACGGTCATCGGTTGGTCGAGCAGCTCTTCGATCATCAGCTCGACCTCGTCCGGCAGCTTGGTGCCCTGGCCCGAGAAGAACTTGATGCCGtt                                <  6:135530/149‑1 (MQ=255)
                                                                                                                   cggccggcGGCATCGTTGATACGCGAAACCTTGCCCAGCTTGCCCGACTCGACAACGGTCATCGGTTGGTCGAGCAGCTCTTCGATCATCAGCTCGACCTCGTCCGGCAGCTTGGTGCCCTGACCCGAGAAGAACTTGATGCCGTTGTc                             <  6:211853/149‑1 (MQ=255)
                                                                                                                    ggccggcGGCATCGTTGATACGCGAAACCTTGCCCAGCTTGCCCGACTCGACAACGGTCATCGGTTGGTCGAGCAGCTCTTCGATCATCAGCTCGACCTCGTCCGGCAGCTTGGTGCCCTGGCCCGAGAAGAACTTGATGCCGTTGTCa                            <  7:2161/149‑1 (MQ=255)
                                                                                                                        ggcggcATCGTTGATACGCGAAACCTTGCCCAGCTTGCCCGACTCGACAACGGTCATCGGTTGGTCGAGCAGCTCTTCGATCATCAGCTCGACCTCGTCCGGCAGCTTGGTGCCCTGGCCCGAGAAGAACTTGATGCCGTTGTCATcag                        >  2:264137/1‑147 (MQ=255)
                                                                                                                           ggcATCGTTGATACGCGAAACCTTGCCCAGCTTGCCCGACTCGACAACGGTCATCGGTTGGTCGAGCAGCTCTTCGATCATCAGCTCGACCTCGTCCGGCAGCTTGGTGCCCTGGCCCGAGAAGAACTTGAt                                      >  1:279250/1‑132 (MQ=255)
                                                                                                                           ggcATCGTTGATACGCGAAACCTTGCCCAGCTTGCCCGACTCGACAACGGTCATCGGTTGGTCGAGCAGCTCTTCGATCATCAGCTCGACCTCGTCCGGCAGCTTGGTGCCCTGGCCCGAGAAGAACTTGAt                                      <  2:279250/132‑1 (MQ=255)
                                                                                                                              aTCGTTGATACGCGAAACCTTGCCCAGCTTGCCCGACTCGACAACGGTCATCGGTTGGTCGAGCAGCTCTTCGATCATCAGCTCGACCTCGTCCGGCAGCTTGGTGCCCTGGCCCGAGAAGAACTTGATGCCGTTGTCATCGTGCGGGt                  >  6:176933/1‑149 (MQ=255)
                                                                                                                              aTCGTTGATACGCGAAACCTTGCCCAGCTTGCACGACTCGACAACGGTCATCGGTTGGTCGAGCAGCTCTTCGATCATCAGCTCGACCTCGTCCGGCAGCTTGGTGCCCTGGCCCGAGAAGAACTTGATGCCGTTGTCATCGTGCGGGt                  >  4:231275/1‑149 (MQ=255)
                                                                                                                                    gATACGCGAAACCTTGCCCAGCTTGCCCGACTCGACAACGGTCATCGGTTGGTCGAGCAGCTCTGCGATCATCAGCTCGACCTCGTCCGGCAGCTTGGTGCCCTGGCCCGAGAAGAACTTGATGCCGTTGTCATCGTGCGGGTTGTGc             <  7:236948/148‑1 (MQ=255)
                                                                                                                                     aTACGCGAAACCTTGCCCAGCTTGCCCGACTCGACAACGGTCATCGGTTGGTCGAGCAGCTCTTCGATCATCAGCTCGACCTCGTCCGGCAGCTTGGTGCCCTGGCCCGAGAAGAACTTGATGCCGTTGTCATCGTGCGGGTTGTGCGa           <  4:46544/149‑1 (MQ=255)
                                                                                                                                       aCGCGAAACCTTGCCCAGCTTGCCCGACTCGACAACGGTCATCGGTTGGTCGAGCAGCTCTTCGATCATCAGCTCGACCTCGTCCGGCAGCTTGGTGCCCTGGCCCGAGAAGAACTTGATGCCGTTGTCATCGTGCGGGTTGTGCGAgg         >  5:348658/1‑149 (MQ=255)
                                                                                                                                         gcgAAACCTTGCCCAGCTTGCCCGACTCGACAACGGTCATCGGTTg                                                                                                              >  6:5438/1‑46 (MQ=255)
                                                                                                                                         gcgAAACCTTGCCCAGCTTGCCCGACTCGACAACGGTCATCGGTTg                                                                                                              <  5:5438/46‑1 (MQ=255)
                                                                                                                                         gcgAAACCTTGCCCAGCTTGCCCGACTCGACAACGGTCATCGGTTGGTCGAGCAGCTCTTCGATCATCAGCTCGACCTCGTCCGGCAGCTTGGTGCCCTGGCCCGAGAAGAACTTGATGCCGTTGTCATCGTGCGGGTTGTGCGAGGCa       >  4:337199/1‑149 (MQ=255)
                                                                                                                                           gAATCCTTGCCCCGCTTGCCCGACTCGACAACGGTCATCGGTTGGTCGAGCAGCTCTGCGTTCATCAGCTCGACCTCGTGCGGCAGCTTGGTGCCGTGGCCCGAGAAGAACTTGATGCCGTTCTCATCGTGCGGGTTGTGCGAGGCACt     <  5:42676/149‑1 (MQ=255)
                                                                                                                                           gAAACCTTGCCCAGCTTGCCCGACTCGACAACGGTCATCGGTTGGTCGAGCAGCTCTTCGATCATCAGCTCGACCTCGTCCGGCAGCTTGGTGCCCTGGCCCGAGAAGAACTTGATGCCGTTGTCATCGTGCg                     >  4:227865/1‑133 (MQ=255)
                                                                                                                                           gAAACCTTGCCCAGCTTGCCCGACTCGACAACGGTCATCGGTTGGTCGAGCAGCTCTTCGATCATCAGCTCGACCTCGTCCGGCAGCTTGGTGCCCTGGCCCGAGAAGAACTTGATGCCGTTGTCATCGTGCg                     <  3:227865/133‑1 (MQ=255)
                                                                                                                                              aCCTTGCCCAGCTTGCCCGACTCGACAACGGTCATCGGTTGGTCGAGCAGCTCTTCGATCATCAGCTCGACCTCGTCCGGCAGCTTGGTGCCCTGGCCCGAGAAGAACTTGATGCCGTTGTCATCGTGCGGGTTGTGCGAGGCACtgat  <  6:348658/149‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                                   
ACGGAATACGCTGGGCGCAACCTTGTAGGTAGCACCATGGGCGCAGTCGACCACCAGCTTGAGGCCTTCGAAGCTGGTGCTGCTCGGCACACTGCTCTTGCAGAATTCGATGTAGCGGCCGGCGGCATCGTTGATACGCGAAACCTTGCCCAGCTTGCCCGACTCGACAACGGTCATCGGTTGGTCGAGCAGCTCTTCGATCATCAGCTCGACCTCGTCCGGCAGCTTGGTGCCCTGGCCCGAGAAGAACTTGATGCCGTTGTCATCGTGCGGGTTGTGCGAGGCACTGAT  >  NC_002947/5363184‑5363474

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑