Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,430,376 A→G 7.8% intergenic (‑58/+103) PP_4769 ← / ← PP_4770 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,430,3760AG 7.8% 217.2 / 6.7 78intergenic (‑58/+103)PP_4769/PP_4770hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (37/34);  new base G (3/3);  total (41/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.67e-01

GGGCGGTGTAGTTGAAATGGCAGCCGTGGGCCTGGCGCACGGTGTAGTAGAGGTTCTGCACATCGGCGAACAGCGCGATCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGA‑GCGGGCGTGCCCGCGAAGGGGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCAGACCTGTCAGACGTAAGAATCGTCGTCCGAGAAGAACCCACCGTCATC  >  NC_002947/5430231‑5430520
                                                                                                                                                 |                                                                                                                                                  
gggCGGTGTAGTTGAAATGGCAGCCGTGGGCCTGGCGCACGGTGTAGTAGAGGTTCTGCACATCGGCGAACAGCGCGATCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGacac                                                                                                                                                 <  3:64765/149‑1 (MQ=255)
 ggCGGTGTAGTTGAAATGGCAGCCGTGGGCCTGGCGCACGGTGTAGTAGAGGTTCTGCACATCGGCGAACAGCGCGATCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACg                                                                                                                                                >  1:110562/1‑149 (MQ=255)
 ggCGGTGTAGTTGAAATGGCAGCCGTGGGCCTGGCGCACGGTGTAGTAGAGGTTCTGCACATCGGCGAACAGCGCGATCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACg                                                                                                                                                >  3:315833/1‑149 (MQ=255)
   cGGTGTAGTTGAAATGGCAGCCGTGGGCCTGGCGCACGGTGTAGTAGAGGTTCTGCACATCGGCGAACAGCGCGATCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGcc                                                                                                                                              >  7:230344/1‑149 (MQ=255)
    ggTGTAGTTGAAATGGCAGCCGTGGGCCTGGCGCACGGTGTAGTAGAGGTTCTGCACATCGGCGAACAGCGCGATCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGccc                                                                                                                                             >  1:105880/1‑149 (MQ=255)
           ttGAAATGGCAGCCGTGGGCCTGGCGCACGGTGTAGTAGAGGTTCTGCACATCGGCGAACAGCGCGATCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCca                                                                                                                                      >  4:74252/1‑149 (MQ=255)
            tGAAATGGCAGCCGTGGGCCTGGCGCACGGTGTAGTAGAGGTTCTGCACATCGGCGAACAGCGCGATCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGC‑‑CCCTTCGCGGGCACGCCCGCTCCcacgg                                                                                                                                   >  4:188043/1‑147 (MQ=255)
             gAAATGGCAGCCGTGGGCCTGGCGCACGGTGTAGTAGAGGTTCTGCACATCGGCGCACAGCGCGATCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCcac                                                                                                                                     >  1:308834/1‑148 (MQ=255)
             gAAATGGCAGCCGTGGGCCTGGCGCACGGTGTAGTAGAGGTTCTGCACATCGGCGAACAGCGCGATCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGGCACGCCCGCTCCcacg                                                                                                                                    >  8:239047/1‑148 (MQ=255)
             gAAATGGCAGCCGTGGGCCTGGCGCACGGTGTAGTAGAGGTTCTGCACATCGGCGAACAGCGCGATCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCca                                                                                                                                      >  3:158047/1‑147 (MQ=255)
             gAAATGGCAGCCGGGGGCCTGGCGCACGGTGTTGTAGAGGTTCTGCTCATCGGCGAACAGCGCGCGCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCcaca                                                                                                                                    <  4:43249/149‑1 (MQ=255)
              aaaTGGCAGCCGTGGGCCTGGCGCACGGTGTAGTAGAGGTTCTGCACATCGGCGAACAGCGCGATCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAg                                                                                                                                   <  5:275035/149‑1 (MQ=255)
                  ggCAGCCGTGGGCCTGGCGCACGGTGTAGTAGAGGTTCTGCACATCGGCGAACAGCGCGATCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAt                                                                                                                               >  3:98633/1‑149 (MQ=255)
                     aGCCGTGGGCCTGGCGCACGGTGTAGTAGAGGTTCTGCACATCGGCGAACAGCGCGATCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑cgc                                                                                                                           <  2:110562/149‑1 (MQ=255)
                       ccGTGGGCCTGGCGCACGGTGTAGTAGAGGTTCTGCACATCGGCGAACAGCGCGATCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑cgcgc                                                                                                                         >  8:26877/1‑149 (MQ=255)
                          tGGGCCTGGCGCACGGTGTAGTAGAGGTTCTGCACATCGGCGAACAGCGCGATCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTa                                                                                                                      <  7:64738/149‑1 (MQ=255)
                               cTGGCGCACGGTGTAGTAGAGGTTCTGCACATCGGCGAACAGCGCGATCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACAccc                                                                                                                 <  2:105880/149‑1 (MQ=255)
                                  gcgcACGGTGTAGTAGAGGTTCTGCACATCGGCGAACAGCGCGATCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGt                                                                                                               <  4:271056/148‑1 (MQ=255)
                                  gcgcACGGTGTAGTAGAGGTTCTGCACATCGGCGAACAGCGCGATCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGt                                                                                                               <  8:230344/148‑1 (MQ=255)
                                   cgcACGGTGTAGTAGAGGTTCTGCACATCGGCGAACAGCGCCTTCATTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCGTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTgg                                                                                                             <  3:204557/149‑1 (MQ=255)
                                     cACGGTGTAGTAGAGGTTCTGCACATCGGCGAACAGCGCGATCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGa                                                                                                           <  4:315833/149‑1 (MQ=255)
                                     cACGGTGTAGTAGAGGTTCTGCACATCGGCGAACAGCGCGATCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGa                                                                                                           <  7:26877/149‑1 (MQ=255)
                                      aCGGTGTAGTAGAGGTTCTGCACATCGGCGAACAGCGCGATCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGC‑‑CCCTTCGCGGGCACGCCCGCTCCCACGGGTGTACGCGCG‑ATACCTGTGGGA‑GCg                                                                                                       <  3:188043/149‑1 (MQ=21)
                                         gtgtAGTAGAGGTTCTGCACATCGGCGAACAGCGCGATCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGA‑GCgg                                                                                                      <  1:63204/149‑1 (MQ=255)
                                         gtgtAGTAGAGGTTCTGCACATCGGCGAACAGCGCGATCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGA‑GCgg                                                                                                      <  6:144552/149‑1 (MQ=255)
                                           gtagtaGAGGTTCTGCACATCGGCGAACAGCGCGATCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCCCGTACACCCGTGGGA‑GCGGGc                                                                                                    >  2:8126/1‑149 (MQ=255)
                                            tagtagAGGTTCTGCACATCGGCGAACAGCGCGATCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGA‑GCGGGc                                                                                                    <  6:8870/148‑1 (MQ=255)
                                            tagtagAGGTTCTGCACATCGGCGAACAGCGCGATCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGA‑GCGGGc                                                                                                    <  4:158047/148‑1 (MQ=255)
                                              gtagAGGTTCTGCACATCGGCGAACAGCGCGATCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGA‑GCGGGCGTg                                                                                                 >  7:225368/1‑149 (MQ=255)
                                               tagAGGTTCTGCACATCGGCGAACAGCGCGATCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGA‑GCGGGCGTGc                                                                                                >  4:150688/1‑149 (MQ=255)
                                                 gagGTTCTGCACATCGGCGAACAGCGCGATCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGCCACGCCCCCGCCCCCCCGGAT‑CGCGCCTACACCCGTGGGAGGCGGGCGGGcc                                                                                               >  8:290491/1‑149 (MQ=255)
                                                   ggTTCTGCACATCGGCGAACAGCGCGATCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGA‑GCGGGCGTGCCcgc                                                                                            <  8:278545/149‑1 (MQ=255)
                                                    gTTCTGCACATCGGCGAACAGCGAGATCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGA‑GCGGGCGTGCCcgc                                                                                            >  8:186574/1‑148 (MQ=255)
                                                       cTGCACATCGGCGAACAGCGCGATCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGGCACGCCCGCTCCCACGGGTGTACGCGCG‑ATACCTGTGGGA‑GCGGGCGTGTCCGCGAAg                                                                                        >  1:242252/1‑149 (MQ=21)
                                                        tGCACATCGGCGAACAGCGCGATCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGA‑GCGGGCGTGCCCGCGAAgg                                                                                       >  2:41008/1‑149 (MQ=255)
                                                         gCACATCGGCGAACAGCGCGATCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGA‑GCGGGCGTGCCCGCGAAggg                                                                                      >  5:133216/1‑149 (MQ=255)
                                                          cacaTCGGCGAACAGCGCGATCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGGCACGCCCGCTCCCACGGGTGTACGCGCG‑ATACCTGTGGGA‑GCGGGCGTGTCCGCGAAg                                                                                        <  2:242252/146‑1 (MQ=21)
                                                          cacaTCGGCGAACAGCGCGATCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGGCACGCCCGCTCCCACGGGTGTACGCGCG‑ATACCTGTGGGA‑GCGGGCGTGTCCGCGAAg                                                                                        <  7:239047/146‑1 (MQ=21)
                                                          cacaTCGGCGAACAGCGCGATCTTTTTCACCTGGCACCCTCATTATTGGCCGGGCGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCCCAGGTAT‑CGCGCGTACACCCGTGGGA‑GCGGGCGTGCCCGCGAAgg                                                                                       <  7:290491/147‑1 (MQ=39)
                                                             aTCGGCGAACAGCGCGATCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGA‑GCGGGCGTGCCCGCGAAGGGgcg                                                                                   >  6:339239/1‑148 (MQ=255)
                                                                    aaCAGCGCGATCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGA‑GCGGGCGTGCCCGCGAAGGGGCGCAAAgcgc                                                                           >  4:246216/1‑149 (MQ=255)
                                                                         cgcgATCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGA‑GCgg                                                                                                      >  6:237470/1‑117 (MQ=255)
                                                                         cgcgATCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGA‑GCgg                                                                                                      <  5:237470/117‑1 (MQ=255)
                                                                           cgATATTTTTCACCTGTGAACATAATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCGGCTCCCACAGGTAT‑CGCGCGTACACCGGTGGGA‑GCGGGCGTGCCCGCGAAGGGGCGCAAAGCGCCCCCGGa                                                                    <  2:308834/149‑1 (MQ=38)
                                                                               cTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGA‑GCGGGCGTGCCCGCGAAGGGGCGCAAAGCGCCCCCGGAAATg                                                                >  5:104169/1‑149 (MQ=255)
                                                                                tttttCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑cgc                                                                                                                           <  1:219022/90‑1 (MQ=255)
                                                                                tttttCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑cgc                                                                                                                           >  2:219022/1‑90 (MQ=255)
                                                                                 ttttCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTgg                                                                                                             >  4:339396/1‑103 (MQ=255)
                                                                                 ttttCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTgg                                                                                                             <  3:339396/103‑1 (MQ=255)
                                                                                   ttCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCTCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGA‑GCGGGCGTGCCCGCGAAGGGGCGCAAAGCGCCCCCGGAAATGCca                                                             <  4:98633/148‑1 (MQ=255)
                                                                                   ttCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGA‑GCGGGCGTGCCCGCGAAGGGGCGCAAAGCGCCCCCGGAAATGCca                                                             <  1:8126/148‑1 (MQ=255)
                                                                                    tCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGA‑GCGGGCGTGCCCGCGAAGGGGCGCAAAGCGCCCCCGGAAATGCcag                                                            <  8:225368/148‑1 (MQ=255)
                                                                                        cTGGGAACCTCATATTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGA‑GCGGGCGTGCCCGCGAAGGGGCGCAAAGCGCCCCCGGAAATGCCAGCAGTa                                                       >  7:67293/1‑149 (MQ=255)
                                                                                         tGGGNACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑AGCGCGTACACCCGTGGGA‑GCGGGCGTGCCCGCGCAGGGGCGCAAAGCGCCCCCGGCAATTCCAGCAGTAt                                                      >  5:31163/1‑149 (MQ=255)
                                                                                         tGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGA‑GCGGGCGTGCCCGCGAAGGGGCGCAAAGCGCCCCCGGAAATGCCAGCAGTAt                                                      >  7:64209/1‑149 (MQ=255)
                                                                                               ccTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGA‑GCGGGc                                                                                                    >  1:315750/1‑97 (MQ=255)
                                                                                               ccTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGA‑GCGGGc                                                                                                    <  2:315750/97‑1 (MQ=255)
                                                                                               ccTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGA‑GCGGGCGTGCCCGCGAAGGGGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCAGa                                                >  6:257528/1‑149 (MQ=255)
                                                                                                 tCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGA‑GCGGGCGTGCCCGCGAAGGGGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCAGAcc                                              >  5:241522/1‑149 (MQ=255)
                                                                                                   aTTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCCCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGA‑GCGGGCGTGCCCGCGAAGGGGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCAGACCTg                                            >  4:177260/1‑149 (MQ=255)
                                                                                                     ttttGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGA‑GCGGGCGTGCCCGCGAAGGGGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCAGACCTGt                                           >  5:215913/1‑148 (MQ=255)
                                                                                                          gCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGA‑GCGGGCGTGCCCGCGAAGGGGCGCAAAGCGCCCCCTGAAATGCCAGCAGTATGCCAGAc                                               <  2:136061/139‑1 (MQ=255)
                                                                                                          gCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGGGGGA‑GCGGGCGTGCCCGCGAAGGGGCGCAAAGCGCCCCCCGAAATGCCAGCAGTATGCCAGAc                                               >  1:136061/1‑139 (MQ=255)
                                                                                                            cTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGA‑GCGGGCGTGCCCGCGAAGGGGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCAGCCCTGTCCGACGt                                    >  4:75135/1‑148 (MQ=255)
                                                                                                                     cGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGA‑GCGGGCGTGCCCGCGAAGGGGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCAGACCTGTCAGACGTAAGAAtcgtc                          <  3:177260/149‑1 (MQ=255)
                                                                                                                      ggggggCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGA‑GCGGGCGTGCCCGCGAAGGGGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCAGACCTGTCAGACGTAAGAAtcgtcg                         <  5:339239/149‑1 (MQ=255)
                                                                                                                      ggggggCTGCTGCGCAGACCTTCGCGGACACCCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGA‑GCCGGCGTGCCCGCGAAGGGGCGCAAAGCGCCCCCCGAAATGCCAGCAGTATGCCAGACCAGTCAGACGTAAGAAacgtcg                         >  8:300029/1‑149 (MQ=255)
                                                                                                                       gggggCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGA‑GCGGGCGTGCCCGCGAAGGGGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCAGACCTGTCAGACGTAAGAATcgtcgt                        <  7:186574/149‑1 (MQ=255)
                                                                                                                        ggggCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGA‑GCGGGCGTGCCCGCGAAGGGGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCAGACCTGTCAGACGTAAGAATCgtcgtc                       <  6:104169/149‑1 (MQ=255)
                                                                                                                             tgctgcGCAGACCTTCGCGGACACGCCCGCTCCCACATGTAT‑CGCGCGTACACCCGTGGGA‑GCGGGCGTGCCCTCGAAGGGGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCAGACCt                                             <  1:222972/122‑1 (MQ=255)
                                                                                                                             tgctgcGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGA‑GCGGGCGTGCCCGCGAAGGGGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCAGACCt                                             >  2:222972/1‑122 (MQ=255)
                                                                                                                             tgctgcGCAGACCTTCGCGGACACGCCCCCACCCACAGGTAT‑AGCGCGTACACCCCTGGGA‑GCGGGCCTGCCCGCGAAGGGGCGCCAAGCGCCCCCGGAAATGCCCCCAGTATGCCAGACCAGTCAGACGTAACAATCGTCGTACgag                   >  8:126121/1‑148 (MQ=255)
                                                                                                                                tgcGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGA‑GCGGGCGTGCCCGCGAAGGGGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCAGACCTGTCAGACGTAAGAATCGTCGTCCGagaaga               <  8:64209/149‑1 (MQ=255)
                                                                                                                                  cgcAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGA‑GCGGGCGTGCCCGCGAAGGGGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCAGACCTGTCAGACGTAAGAATCGTCGTCCGAGAAGAAc             <  6:241522/149‑1 (MQ=255)
                                                                                                                                     aGACCTTCGCGGACCCCCCCCCAACCACACGTAA‑ACCGCGTAAACCCGTGGGA‑GCGGGCGTGCCCGCCAACGGGCGCCAAGCGCCCCCGGAAATGCCCCCAGTAATCCAGACCTGTCAGACCGAAGAATAGTAGTACGAGAAGAAcccc          >  4:168867/1‑148 (MQ=255)
                                                                                                                                       aCCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGA‑GCGGGCGTGCCCGCGAAGGGGCGCAAAGCGCCCCCGGAAATGCCAGCAGTCTGCCAGACCTGTCAGACGTAAGAATCGTCGTCCGAGAAGAACCCAcc        >  4:112702/1‑149 (MQ=255)
                                                                                                                                         cTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGA‑GCGGGCGTGCCCGCGAAGGGGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCAGACCTGTCAGACGTAAGAATCGTCGTcc                      <  8:298849/133‑1 (MQ=255)
                                                                                                                                         cTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGA‑GCGGGCGTGCCCGCGAAGGGGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCAGACCTGTCAGACGTAAGAATCGTCGTcc                      >  7:298849/1‑133 (MQ=255)
                                                                                                                                             gcgGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGA‑GCGGGCGTGCCCGCGAAGGGGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCAGACCTGTCAGACGTAAGAATCGTCGTCCGAGAAGAACCCACCGTCATc  <  1:41008/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                  
GGGCGGTGTAGTTGAAATGGCAGCCGTGGGCCTGGCGCACGGTGTAGTAGAGGTTCTGCACATCGGCGAACAGCGCGATCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGA‑GCGGGCGTGCCCGCGAAGGGGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCAGACCTGTCAGACGTAAGAATCGTCGTCCGAGAAGAACCCACCGTCATC  >  NC_002947/5430231‑5430520

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑