Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,459,561 C→A 10.9% intergenic (+19/‑21) PP_4795 → / → holA lipoprotein/DNA polymerase III subunit delta

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,459,5610CA10.9% 150.8 / 7.5 53intergenic (+19/‑21)PP_4795/holAlipoprotein/DNA polymerase III subunit delta
Reads supporting (aligned to +/- strand):  ref base C (22/25);  new base A (2/4);  total (24/29)
Fisher's exact test for biased strand distribution p-value = 6.78e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.74e-01

CCCAACTGGATGAGCTGCAGCGTAAAGCAGACGAGCGCGCCCAAGCCGAAGCTGCCGCGCTGGAGGCTGCCCGCCGCCAGCAAGCCGAGACGCCTCAGCAATCGCCACTGGAAGTAC‑CGGGCAACTAAGCCCGCGAGGGGCGCCCTCGGGCGCCCCGCCTGTTCCCCATGAAACTCGCCCCCGCCCAACTCAACAAGCACCTGCAAGGCGCCCTGGCACCGGTCTACGTGGTCAGTGGCGACGACCCGCTGCTGTGCCAGGAAGCCGCCGACGCCATCCGCAATG  >  NC_002947/5459415‑5459699
                                                                                                                                                   |                                                                                                                                          
cccAACTGGATGAGCTGCAGCGTAAAGCAGACGAGCGCGCCCAAGCCGAAGCTGCCGCGCTGGAGGCTGCCCGCCGCCAGCAAGCCGAGACGCCTCAGCAATCGCCACTGGAAGTAC‑CGGGCAACTAAGCCCGCGAGGGGCGCCCTCgg                                                                                                                                          >  2:302428/1‑149 (MQ=255)
 ccAACTGGATGAGCTGCAGCGTAAAGCAGACGAGCGCGCCCAAGCCGAAGCTGCCGCGCTGGAGGCTGCCCGCCGCCAGCAAGCCGAGACGCCTCAGCAATCGCCACTGGAAGTAC‑CGGGCAACTAAGCCCGCGAGGGGCGCCCTCggg                                                                                                                                         >  3:353284/1‑149 (MQ=255)
 ccAACTGGATGAGCTGCAGCGTAAAGCAGACGAGCGCGCCCAAGCCGAAGCTGCCGCGCTGGAGGCTGCCCGCCGCCAGCAAGCCGAGACGCCTCAGCAATCGCCACTGGAAGTAC‑CGGGCAACTAAGCCCGCGAGGGGCGCCCTCggg                                                                                                                                         <  1:60475/149‑1 (MQ=255)
    aCTGGATGAGCTGCAGCGTAAAGCAGACGAGCGCGCCCAAGCCGAAGCTGCCGCGCTGGAGGCTGCCCGCCGCCAGCAAGCCGAGACGCCTCAGCAATCGCCACTGGAAGTAC‑CGGGCAACTAAGCCCGCGAGGGGCGCCCTCGGgcgc                                                                                                                                      >  8:81346/1‑149 (MQ=255)
     cTGGATGTGCTGCAGCGTAAAGCAGACGAGCGCGCCCAAGCCGAAGCTGCCGCGCTGGAGGCTGCCCGCCGCCAGCAAGCCGAGACGCCTCAGCAATCGCCACTGGAAGTAC‑CGGGCAACTAAGCCCGCGAGGGGCGCCCTCGGGCGcc                                                                                                                                     >  6:351912/1‑149 (MQ=255)
        gATGAGCTGCAGCGTAAAGCAGACGAGCGCGCCCAAGCCGAAGCTGCCGCGCTGGAGGCTGCCCGCCGCCAGCAAGCCGAGACGCCTCAGCAATCGCCACTGGAAGTAC‑CGGGCAACTAAGCCCGCGAGGGGCGCCCGAGGGCGccc                                                                                                                                    <  7:256332/147‑1 (MQ=255)
         ctGAGCTGCAGCGTAAAGCAGACGAGCGCGCCCAAGCCGAAGCTGCCGCGCTGGAGGCTGCCCGCCGCCAGCAAGCCGAGACGCCTCAGCAATCGCCACTGGAAGTAC‑CGGGCAACTAAGCCCGCGAGGGGCGCCCGAGGGCGcccctc                                                                                                                                 <  7:294248/148‑3 (MQ=255)
         aTGAGCTGCAGCGTAAAGCAGACGAGCGCGCCCAAGCCGAAGCTGCCGCGCTGGAGGCTGCCCGCCGCCAGCAAGCCGAGACGCCTCAGCAATCGCCACTGGAAGTAC‑CGGGCAACTAAGCCCGCGAGGGGCGCCCTCGGGCGCCCCGc                                                                                                                                 <  3:314935/149‑1 (MQ=255)
            aGCTGCAGCGTAAAGCAGACGAGCGCGCCCAAGCCGAAGCTGCCGCGCTGGAGGCTGCCCGCCGCCAGCAAGCCGAGACGCCTCAGCAATCGCCACTGGAAGTAC‑CGGGCAACTAAGCCCGCGAGGGGCGCCCTCGGGCGCCCCGCCt                                                                                                                               <  7:24674/148‑1 (MQ=255)
                 cAGCGTAAAGCAGACGAGCGCGCCCAAGCCGAAGCTGCCGCGCTGGAGGCTGCCCGCCGCCAGCAAGCCGAGACGCCTCAGCAATCGCCACTGGAAGTAC‑CGGGCAACTAAGCCCGCGAGGGGCGCCCTCGGGCGCCCCGCCTGTTccc                                                                                                                         >  2:200109/1‑149 (MQ=255)
                  aGCGTAAAGCAGACGAGCGCGCCCAAGCCGAAGCTGCCGCGCTGGAGGCTGCCCGCCGCCAGCAAGCCGAGACGCCTCAGCAATCGCCACTGGAAGTAC‑CGGGCAACTAAGCCCGCGAGGGGCGCCCTCGGGCGCCCCGCCTGTTcccc                                                                                                                        <  1:286173/149‑1 (MQ=255)
                     gTAAAGCAGACGAGCGCGCCCAAGCCGAAGCTGCCGCGCTGGAGGCTGCCCGCCGCCACCAAGCCGAGACGCCGCAGCAATCGCCACTGGAAGTAC‑CGGGCAACTAAGCCCGCGAGGGGCGCCCTCGGGCGCCCCGCCTGTTCCCCATg                                                                                                                     <  3:142929/149‑1 (MQ=255)
                      tAAAGCAGACGAGCGCGCCCAAGCCGAAGCTGCCGCGCTGGAGGCTGCCCGCCGCCAGCAAGCCGAGACGCCTCAGCAATCGCCACTGGAAGTAC‑CGGGCAACTAAGCCCGCGAGGGGCGCCCTCGGGCGCCCCGCCTGTTCCCCATg                                                                                                                     >  5:106474/1‑148 (MQ=255)
                       aaaGCAGACGAGCGCGCCCAAGCCGAAGCTGCCGAGCTGGAGGCTGCCCGCCGCCAGCAAGCCGAGACGCCTCAGCAATCGCCACTGGAAGTAC‑CGGGCAACTAAGCCCGCCAGGGGCGCCCTCGGGCGCCCCGCCGGGTGCCCCTGa                                                                                                                    >  8:68886/1‑148 (MQ=255)
                        aaGCAGACGAGCGCGCCCAAGCCGAAGCTGCCGCGCTGGAGGCTGCCCGCCGCCAGCAAGCCGAGACGCCTCAGCAATCGCCACTGGAAGTAC‑CGGGCAACTAAGCCCGCGAGGGGCGCCCTCGGGCGCCCCGCCTGTTCCCCATGaaa                                                                                                                  <  6:5172/149‑1 (MQ=255)
                        aaGCAGACGACCGCGCCCAAGCCGAACCTGCCGCGCTGGTTGCTGCCCGCCGCCAGCAATCCGAGACGCCGCATCAATCCGCACTGGAATTACTCGGGCAACTAAGCCCGCGAGGGGCGCCCTCGGGCGCCCCGCCTGTTCCCCATGa                                                                                                                    <  1:276873/148‑1 (MQ=255)
                             gACGAGCGCGCCCAAGCCGAAGCTGCCGCGCTGGAGGCTGCCCGCCGCCAGCAAGCCGAGACGCCTCAGCAATCGCCACTGGAAGTAC‑CGGGCAACTAAGCCCGCGAGGGGCGCCCTCGGGCGCCCCGCCTGTTCCCCATGAAACTCGc                                                                                                             <  6:120004/149‑1 (MQ=255)
                                gAGCGCGCCCAAGCCGAAGCTGCCGCGCTGGAGGCTGCCCGCCGCCAGCAAGCCGAGACGCCTCAGCAATCGCCACTGGAAGTAC‑CGGGCAACTAAGCCCGCGAGGGGCGCCCTCGGGCGCCCCGCCTGTTcccc                                                                                                                        <  4:146622/135‑1 (MQ=255)
                                  gcgcgcCCAAGCCGAAGCTGCCGCGCTGGAGGCTGCCCGCCGCCAGCAAGCCGAGACGCCTCAGCAATCGCCACTGGAAGTAC‑CGGGCAACTAAGCCCGCGAGGGGCGCCCGAGGGCGccc                                                                                                                                    <  1:97714/121‑1 (MQ=255)
                                  gcgcgcCCAAGCCGAAGCTGCCGCGCTGGAGGCTGCCCGCCGCCAGCAAGCCGAGACGCCTCAGCAATCGCCACTGGAAGTAC‑CGGGCAACTAAGCCCGCGAGGGGCGCCCGAGGGCGccc                                                                                                                                    >  2:97714/1‑121 (MQ=255)
                                          aaGCCGAAGCTGCCGCGCTGGAGGCTGCCCGCCGCCAGCAAGCCGAGACGCCTCAGCAATCGCCACTGGAAGTAC‑CGGGCAACTAAGCCCGCGAGGGGCGCCCGAGGGCGcccc                                                                                                                                   <  6:129274/114‑1 (MQ=255)
                                          aaGCCGAAGCTGCCGCGCTGGAGGCTGCCCGCCGCCAGCAAGCCGAGACGCCTCAGCAATCGCCACTGGAAGTAC‑CGGGCAACTAAGCCCGCGAGGGGCGCCCGAGGGCGcccc                                                                                                                                   >  5:129274/1‑114 (MQ=255)
                                            gCCGAAGCTGCCGCGCTGGAGGCTGCCCGCCGCCAGCAAGCCGAGACGCCTCAGCAATCGCCACTGGAAGTAC‑CGGGCAACTAAGCCCGCGAGGGGCGCCCTCGGGCGCCCCGCCTGTTCCCCATGAAACTCGccccc                                                                                                         <  7:138942/138‑1 (MQ=255)
                                            gCCGAAGCTGCCGCGCTGGAGGCTGCCCGCCGCCAGCAAGCCGAGACGCCTCAGCAATCGCCACTGGAAGTAC‑CGGGCAACTAAGCCCGCGAGGGGCGCCCTCGGGCGCCCCGCCTGTTCCCCATGAAACTCGccccc                                                                                                         >  8:138942/1‑138 (MQ=255)
                                            gCCGAAGCTGCCGCGCTGGAGGCTGCCCGCCGCCAGCAAGCCGAGACGCCTCAGCAATCGCCACTGGAAGTAC‑CGGGCAACTAAGCCCGCGAGGGGCGCCCTCGGGCGCCCCGCCTGTTCCCCATGAAACTCGCCCCCGCCCAACTcaa                                                                                              <  5:351912/149‑1 (MQ=255)
                                             ccGAAGCTGCCGCGCTGGAGGCTGCCCGCCGCCAGCAAGCCGAGACGCCTCAGCAATCGCCACTGGAAGTAC‑CGGGCAACTAAGCCCGCGAGGGGCGCCCTCGGGCGCCCCGCCTGTTCCCCATGAAACTCGCCCCCGCCCAACTcaac                                                                                             <  7:81346/149‑1 (MQ=255)
                                                   cTGCCGCGCTGGAGGCTGCCCGCCGCCAGCAAGCCGAGACGCTTTTGCAATCGCCACTGGAAGTAC‑CGGGCAACTAAGCCCGCGAGGGGCGCCCTCGGGCGCCCCGCCTGTTCCCCATGAAACTCGCCCCCGCCCAACTCAACAAGCAc                                                                                       <  1:302428/149‑1 (MQ=255)
                                                     gCCGCGCTGGAGGCTTCCCGCCGCCAGCAAGCCGAGACGCCTCAGCAATCGCCACTGGAAGTAC‑CCGGCAACTAAGCCCGCGACGCGCGCCCTCGGGCGCCCCCACTCTTCCCCATGAAACTCGCCCCCGCCCAACAGAACAAGCACCt                                                                                     >  8:270308/1‑149 (MQ=255)
                                                      ccGCGCTGGAGGCTGCCCGCCGCCAGCAAGCCGAGACGCCTCAGCAATCGCCACTGGAAGTAC‑CGGGCAACTAAGCCCGCGAGGGGCGCCCTCGGGCGGCCCGCCAGTTGCCCATGAAACACCCCCCCCGCCAACACAACAAGCAccag                                                                                    >  8:345755/1‑147 (MQ=255)
                                                      ccGCGCTGGAGGCTGCCCGCCGCCAGCAAGCCGAGACGCCTCAGCAATCGCCACTGGAAGTAC‑CGGGCAACTAAGCCCGCGAGGGGCGCCCTCGGGCGCCCCGCCTGTTCCCCATGAAACTCGCCCCCGCCCAACTCAACAAGCACCTg                                                                                    <  4:194703/149‑1 (MQ=255)
                                                      ccGCGCTGGAGGCTGCCCGCCGCCAGCAAGCCGAGACGCCTCAGCAATCGCCACTGGAAGTAC‑CGGGCAACTAAGCCCGCGAGGGGCGCCCTCGCGCGCCCCCCCAGGTCACAAATAAAAAAGCCCCCCCCCCACACAAAAAACAccag                                                                                    >  4:160630/1‑147 (MQ=255)
                                                              gAGGCTGCCCGCCGCCAGCAAGCCGAGACGCCTCAGCAATCGCCACTGGAAGTAC‑CGGGCAACTAAGCCCGCGAGGGGCGCCCTCGGGCGCCCCGCCTGTTCCCCCTGAAACTCGCCCCCGCCCCACTCAACAAGCACCAGCCAGgcgc                                                                            >  2:32966/1‑149 (MQ=255)
                                                              gAGGCTGCCCGCCGCCAGCAAGCCGAGACGCCTCAGCAATCGCCACTGGAAGTAC‑CGGGCAACTAAGCCCGCGAGGGGCGCCCTCGGGCGCCCCGCCTGTTCCCCATGAAACTCGCCCCCGCCCAACTCAACAAGCACCTGCAAGgcgc                                                                            <  3:202114/149‑1 (MQ=255)
                                                                 gCTGCCCGCCGCCAGCAAGCCGAGACGCCTCAGCAATCGCCACTGGAAGTAC‑CGGGCAACTAAGCCCGCGAGGGGCGCCCTCGGGCGCCCCGCCTGTTCCCCATGAAACTCGCCCCCGCCCAACTCAACAAGCACCTGCAAGGCGCCCt                                                                         <  4:353284/149‑1 (MQ=255)
                                                                   tGCCCGCCGCCAGCAAGCCGAGACGCCTCAGGAATCGCCACTGGAAGTAC‑CGGGCAACTAAGCCCGCGAGGGGCGCCCTCGGGCGCCCCGCCTGTTCCCCATGAAACTCGCCCCCGCCCaa                                                                                                   <  3:159839/121‑1 (MQ=255)
                                                                   tGCCCGCCGCCAGCAAGCCGAGACGCCTCAGCAATCGCCACTGGAAGTAC‑CGGGCAACTAAGCCCGCGAGGGGCGCCCTCGGGCGCCCCGCCTGTTCCCCATGAAACTCGCCCCCGCCCAACTCAACAAGCACCt                                                                                     <  3:85874/135‑1 (MQ=255)
                                                                   tGCCCGCCGCCAGCAAGCCGAGACGCCTCAGCAATCGCCACTGGAAGTAC‑CGGGCAACTAAGCCCGCGAGGGGCGCCCTCGGGCGCCCCGCCTGTTACCCAAGAAACTAGCCCCCCCCCAACAcaacaaacccca                                                                                     >  4:85874/1‑129 (MQ=255)
                                                                          cgccAGCAAGCCGAGACGCCTCAGCAATCGCCACTGGAAGTAC‑CGGGCAACTAAGCCCGCGAGGGGCGCCCTCGGGCGCCCCGCCTGTTCCCCATGAAACTCGCCCCCGCCCAACTCAACAAGCACCTGCAAGGCGCCCTGGCACCGGt                                                                <  7:68886/149‑1 (MQ=255)
                                                                               gCAAGCCGAGACGCCTCAGCAATCGCCACTGGAAGTAC‑CGGGCAACTAAGCCCGCGAGGGGCGCCCTCGGGCGCCCCGCCGGTTCCCCATGAAACTCGCCCCCGCCCCACTCAACAAGCCCCAGCCAGGCGCCCGGGCACCGGGCTACg                                                           >  6:207831/1‑149 (MQ=255)
                                                                                 aaGCCGAGACGCCTCAGCAATCGCCACTGGAAGTAC‑CGGGCAACTAAGCCCGCGAGGGGCGCCCTCGGGCGCCCCGCCTGTTCCCCATGAAACTCGCCCCCGCCCAACTCAACAAGCACCTGCAAGGCGCCCTGGCACCGGTCTACGTg                                                         >  2:98822/1‑149 (MQ=255)
                                                                                          cGCCTCAGCAATCGCCACTGGAAGTAC‑CGGGCAACTAAGCCCGCGAGGGGCGCCCTCGGGCGCCCCGCCTGTTCCCCATGAAACTCGCCCCCGCCCAACTCAACAAGCACCTGCAAGGCGCCCTGGCACCGGTCTACGTGGTCAGTGGc                                                <  1:200109/149‑1 (MQ=255)
                                                                                             cTCAGCAATCGCCACTGGAAGTAC‑CGGGCAACTAAGCCCGCGAGGGGCGCCCTCGGGCGCCCCGCCTGTTCCCCATGAAACTAGCCCCCCCCCAACACAACAAGCACCTGCAAGGCGCCCAGGCCCCGGTCTAAGTGGTCAGTGGcgac                                             >  8:112316/1‑149 (MQ=255)
                                                                                                          aCTGGAAGTAC‑CGGGCAACTAAGCCCGCGAGGGGCGCCCTCGGGCGCCCCGCCTGTTCCCCATGAAACTCGCCCCCGCCCCACGCCACAACCACCTGCAAGGCGCCCGGGCCCCGGTGTACGTGGTCAGTGGCGCCGCCCCGCTGCggt                                >  7:240842/1‑149 (MQ=255)
                                                                                                              gAAGTAC‑CGGGCAACTAAGCCCGCGAGGGGCGCCCTCGGGCGCCCCGCCTGTTCCCCATGAAACGCGCCCCCGCCCAACTCAACAAGCACCTGCAAGGCGCCCTGGCACCGGTCTACGTGGTCAGTGGCGACGACCCGCTGCTGTGCCa                            <  7:345755/149‑1 (MQ=255)
                                                                                                                   aC‑CGGGCAACTAAGCCCGCGAGGGGCGCCCTCGGGCGCCCCGCCTGTTCCCCATGAAACTCGCCCCCGCCCCACTCAACAAGCACCGGCAAGGCGCCCAGGCCCCGGTGTTCGTGGTCAGTGGCGGCGACCCGCGGCTGTGGCAGGGAg                       >  5:165858/1‑149 (MQ=255)
                                                                                                                    c‑cGGGCAACTAAGCCCGCGAGGGGCGCCCTCGGGCGCCCCGCCTGTTCCCCATGAAACTCGCCCCCGCCCAACTCAACAAGCACCTGCAAGGCGCCCTGGCACCGGTCTACGTGGTCAGTGGCGACGACCCGCTGCTGTGCCAgg                          <  7:18751/145‑1 (MQ=255)
                                                                                                                    c‑cGGGCAACTAAGCCCGCGAGGGGCGCCCTCGGGCGCCCCGCCTGTTACCCATGAAACTCGCCCCCGCCCCACTCAACAAGCACCAGCAAGGCGCCCAGGCACCGGTCTACGTGGTCAGTGGCGACGCCCCGCAGCTGTGCCAGGAAg                       >  8:18751/1‑148 (MQ=255)
                                                                                                                           aaCTAAGCCCGCGAGGGGCGCCCTCGGGCGCCCCGCCTGTTGCCCATGAAACTCGCCCCCGCCCCACTCAACAAGCACCAGCAAGGCGCCCTGGCCCCGGGCTACGTGGGCAGTGGGGGCGCCCCGCTGGGGTGGCCGGAAGCCGCCGa                >  1:308203/1‑149 (MQ=255)
                                                                                                                            aCTAAGCCCGCGAGGGGCGCCCTCGGGCGCCCCGCCTGTTCCCCATGAAACTCGCCCCCGCCCAACTCAACAAGCACCTGCAAGGCGCCCTGGCACCGGTCTACGTGGTCAGTGGCGACGACCCGCTGCTGTGCCAGGAAGCCGCCGAc               <  3:298793/149‑1 (MQ=255)
                                                                                                                              tAAGCCCGCGAGGGGCGCCCTCGGGCGCCCCGCCTGTTCCCCATGAAACTCGCCCCCGCCCAACTCAACAAGCACCTGCAAGGCGCCCTGGCACCGGTCTACGTGGTCAGTg                                                  <  1:207885/112‑1 (MQ=255)
                                                                                                                                  cccGCGAGGGGCGCCCTCGGGCGCCCCGCCTGTTCCCCATGAAACTCGCCCCCGCCCAACTAAACAAGCACCTGCAAGGCGCCCTGGCACCGGTCTACGTGGTCAGTGGCGACGACCCGCTGCTGTGCCAGGAAGCCGCCGACGCCATc         >  4:277989/1‑149 (MQ=255)
                                                                                                                                    cgcgAGGGGCGCCCTCGGGCGCCCCGCCTGTTCCCCATGAAACTCGCCCCCGCCCAACTCAACAAGCACCTGCAAGGCGCCCTGGCACCGGTCTACGTGGTCAGTGGCGACGACCCGCTGCTGTGCCAGGAAGCCGCCGACGCCATCCg       <  7:112316/149‑1 (MQ=255)
                                                                                                                                         ggggCGCCCTCGGGCGCCCCGCCTGTTCCCCATGAAACTCGCCCCCGCCCAACTCAACAAGCACCTGCAAGGCGCCCTGGCACCGGTCTACGTGGTCAGTGGCGACGACCCGCTGCTGTGCCAGGAAGCCGCCGACGCCATCCGCAATg  >  2:41410/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                          
CCCAACTGGATGAGCTGCAGCGTAAAGCAGACGAGCGCGCCCAAGCCGAAGCTGCCGCGCTGGAGGCTGCCCGCCGCCAGCAAGCCGAGACGCCTCAGCAATCGCCACTGGAAGTAC‑CGGGCAACTAAGCCCGCGAGGGGCGCCCTCGGGCGCCCCGCCTGTTCCCCATGAAACTCGCCCCCGCCCAACTCAACAAGCACCTGCAAGGCGCCCTGGCACCGGTCTACGTGGTCAGTGGCGACGACCCGCTGCTGTGCCAGGAAGCCGCCGACGCCATCCGCAATG  >  NC_002947/5459415‑5459699

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑