Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,462,393 T→G 12.3% intergenic (+16/+18) PP_4798 → / ← PP_4799 membrane‑bound lytic murein transglycosylase/muramoyltetrapeptide carboxypeptidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,462,3930TG12.3% 164.2 / 10.6 63intergenic (+16/+18)PP_4798/PP_4799membrane‑bound lytic murein transglycosylase/muramoyltetrapeptide carboxypeptidase
Reads supporting (aligned to +/- strand):  ref base T (25/30);  new base G (4/4);  total (29/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGGGTAATGCCGATGGCATTATCGGGGCCAATACCCGCAAGGCAATCCGCAATGCCCAGCAAGGGCTGGGATGGCCGGCGGATGGCTACCCGACCCACAAGCTGCTTGAGAGCCTGCGCCAGCAGTAAGCATTCCGGGGCCGCTGCGCGGCCCCGGTAATCTCACGCCCTGAACAGATCCTGCTCCAGCACCAGTGTTTGCTGAACACTGTCCAGCCTCACCCGAGCCCCCAGGGGCAAGCATACATTCGGGTCACAGTGCCCGCTGCGCCAACCCGCCAGCACCGGCA  >  NC_002947/5462250‑5462538
                                                                                                                                               |                                                                                                                                                 
cGGGTAATGCCGATGGCATTATCGGGGCCAATACCCGCAAGGCAATCCGCAATGCCCAGCAAGGGCTGGGATGGCCGGCGGATGGCTACCCGACCCACAAGCTGCTTGAGAGCCTGCGCCAGCAGTAAGCATTCCGGGGCCGCTgcgc                                                                                                                                               <  6:140947/148‑1 (MQ=255)
      aTGCCGTTGGCATTATCGGGGCCAATACCCGAAAGGCAATCCGAATTGCCCAGCATGGGCTGGGATGGCCGGCGGATGGCTACCGGACCCACAAGCTGTTTGAGAGCCTGCGCCAGCAGTATGCTTTCGGGGGCCGCGCAGCGGcccc                                                                                                                                         <  2:339563/148‑1 (MQ=255)
       tGCCGATGGCATTATCGGGGCCAATACCCGCAAGGCAATCCGCAATGCCCAGCAAGGGCTGGGATGGCCGGCGGATGGCTACCCGACCCACAAGCTGCTTGAGAGCCTGCGCCAGCAGTAAGCATTCCGGGGCCGCTGCGCGGCCCCgg                                                                                                                                       >  4:116347/1‑149 (MQ=255)
       tGCCGATGGCATTATCGGGGCCAATACCCGCAAGGCAATCCGCAATGCCCAGCAAGGGCTGGGATGGCCGGCGGATGGCTACCCGACCCACAAGCTGCTTGAGAGCCTGCGCCAGCAGTAAGCATTCCGGGGCCGCTGCGCGGCCCCgg                                                                                                                                       <  1:172991/149‑1 (MQ=255)
       tGCCGATGGCATTATCGGGGCCAATACCCGCAAGGCAATCCGCAATGCCCAGCAAGGGCTGGGATGGCCGGCGGATGGCTACCCGACCCACAAGCTGCTTGAGAGCCTGCGCCAGCAGTAAGCATTCCGGGGCCGCTGCGCGGCCCCgg                                                                                                                                       <  3:116347/149‑1 (MQ=255)
       tGCCGATGGCATTATCGGGGCCAATACCCGCAAGGCAATCCGCAATGCCCAGCAAGGGCTGGGATGGCCGGCGGATGGCTACCCGACCCACAAGCTGCTTGAGAGCCTGCGCCAGCAGTAAGCATTCCGGGGCCGCGCAGCGGCCCCgg                                                                                                                                       <  2:51193/149‑1 (MQ=255)
           gATGGCATTATCGGGGCCAATACCCGCAAGGCAATCCGCAATGCCCAGCAAGGGCTGGGATGGCCGGCGGATGGCTACCCGACCCACAAGCTGCTTGAGAGCCTGCGCCAGCAGTAAGCATTCCGGGGCCGCTGCGCGGCCCCGGTAAt                                                                                                                                   <  4:297790/149‑1 (MQ=255)
           gATGGCATTATCGGGGCCAATACCCGCAAGGCAATCCGCAATGCCCAGCAAGGGCTGGGATGGCCGGCGGATGGCTACCCGACCCACAAGCTGCTTGAGAGCCTGCGCCAGCAGTAAGCATTCCGGGGCCGCTGCGCGGCCCCGGTAAt                                                                                                                                   <  2:6543/149‑1 (MQ=255)
                 aTTATCGGGGCCAATACCCGAAAGGCAATCCGCAATGCCCAGCAAGGGCTGGGATGGCAGGCGGATGGCTACCCGACCCACAAGCTGCTTGAGAGCCTGCGCCAGCAGTAAGCATTCCGGGGCCGCTGCGCGGCCCCGGTAATCTCACg                                                                                                                             >  5:73614/1‑149 (MQ=255)
                    aTCGGGGCCAATACCCGCAAGGCAATCCGCAATGCCCAGCAAGGGCTGGGATGGCCGGCGGATGGCTACCCGACCCACAAGCTGCTTGAGAGCCTGCGCCAGCAGTAAGCATTCCGGGGCCGCTGCGCGGCCCCGGTAATCTCACGcc                                                                                                                           <  4:226790/148‑1 (MQ=255)
                    aTCGGGGCCAATACCCGCAAGGCAATCCGCAATGCCCAGCAAGGGCTGGGATGGCCGGCGGATGGCTACCCGACCCACAAGCTGCTTGAGAGCCTGCGCCAGCAGTAAGCATTCCGGGGCCGCTGCGCGGCCCCGGTAATCTCACGcc                                                                                                                           <  2:33385/148‑1 (MQ=255)
                     tCGGGGCCAATACCCGCAAGGCAATCCGCAATGCCCAGCAAGGGCTGGGATGGCCGGCGGATGGCTACCCGACCCACAAGCTGCTTGAGAGCCTGCGCCAGCAGTAAGCATTCCGGGGCCGCTGCGCGGCCCCGGTAATCTCACGCCCt                                                                                                                         <  6:354739/149‑1 (MQ=255)
                       ggggCCAATACCCGCATGGCAATCCGCAATGCCCAGCAAGGGCTGGGATGGCCGGCGGATGGCTACCCGACCCACAAGCTGCTTGAGAGCCTGCGCCAGCAGTAAGCATTCCGGGGCCGCTGCGCGGCCCCGGTAATCTCACGCCCTg                                                                                                                        >  6:152802/1‑148 (MQ=255)
                       ggggCCAATACCCGCAAGGCAATCCGCAATGCCCAGCAAGGGCTGGGATGGCCGGCGGATGGCTACCCGACCCACAAGCTGCTTGAGAGCCTGCGCCAGCAGTAAGCATTCCGGGGCCGCTGCGCGGCCCCGGTAATCTCACGCCCTg                                                                                                                        >  5:96264/1‑148 (MQ=255)
                         ggCCAATACCCGCAAGGCAATCCGCAATGCCCAGCAAGGGCTGGGATGGCCGGCGGATGGCTACCCGACCCACAAGCTGCTTGAGAGCCTGCGCCAGCAGTAAGCATTCCGGGGCCGCTGCGCGGCCCCGGTAATCTCACGCCCTGAAc                                                                                                                     <  2:233013/149‑1 (MQ=255)
                          gCCAATACCCGCAAGGCAATCCGCAATGCCCAGCAAGGGCTGGGATGGCCGGCGGATGGCTACCCGACCCACAAGCTGCTTGAGAGCCTGCGCCAGCAGTAAGCATTCCGGGGCCGCTGCGCGGCCCCGGTAATCTCACGCCCTGAACa                                                                                                                    >  3:47958/1‑149 (MQ=255)
                           ccAATAGCCGCAAGGCAATCCGCAATGCCCAGCATGGGCTGGGATGGCCGGCGGATGGCTACCCGACCCACAAGCTGCTTGAGAGCCTGCGCCAGCAGTAAGCATTCCGGGGCCGCTGCGCGGCCCCGGTAATCTCACGCCCTGAACAg                                                                                                                   <  4:229501/149‑1 (MQ=255)
                                aCCCGCAAGGCAATCCGCAATGCCCAGCAAGGGCTGGGATGGCCGGCGGATGGCTACCCGACCCACAAGCTGCTTGAGAGCCTGCGCCAGCAGTAAGCATTCCGGGGCCGCTGCGCGGCCCCGGTAATCTCACGCCCTGAACAGATCCt                                                                                                              >  1:299410/1‑149 (MQ=255)
                                  ccGCATGGCAATCCGCAATGCCCAGCAAGGGCTGGGATGGCCGGCGGATGGCTACCCGACCCACAAGCTGCTTGAGAGCCTGCGCCAGCAGTAAGCATTCCGGGGCCGCTGCGCGGCCCCGGTAATCTCACGCCCTGAACAGATCCTGc                                                                                                            <  5:152802/149‑1 (MQ=255)
                                   cGCAAGGCAATCCGCAATGCCCAGCAAGGGCTGGGATGGCCGGCGGATGGCTACCCGACCCACAAGCTGCTTGAGAGCCTGCGCCAGCAGTAAGCATTCCGGGGCCGCTGCGCGGCCCCGGTAATCTCACGCCCTGAACAGATCCTGCt                                                                                                           >  4:74705/1‑149 (MQ=255)
                                        ggCAATCCGCAATGCCCAGCAAGGGCTGGGATGGCCGGCGGATGGCTACCCGACCCACAAGCTGCTTGAGAGCCTGCGCCAGCAGTAAGCATTCCGGGGCCGCTGCGCGGCCCCGGTAATCTCACGCCCTGAACAGATCCTGCTCCAg                                                                                                       >  7:271212/1‑148 (MQ=255)
                                           aaTCCGCAATGCCCAGCAAGGGCTGGGATGGCCGGCGGATGGCTACCCGACCCACAAGCTGCTTGAGAGCCTGCGCCAGCAGTAAGCATTCCGGGGCCGCTGCGCGGCCCCGGTAATCTCACGCCCTGAACAGATCCTGCTCCAGCAc                                                                                                    >  7:262285/1‑148 (MQ=255)
                                                    tGCCCAGCAAGGGCTGGGATGGCCGGCGGATGGCTACCCGACCCACAAGCTGCTTGAGAGCCTGCGCCAGCAGTAAGCATTCCGGGGCCGCTGCGCGGCCCCGGTAATCTCACGCCCTGAACAGATCCTGCTCCAGCACCAGTGTTTGc                                                                                          <  2:63252/149‑1 (MQ=255)
                                                      cccAGCAAGGGCTGGGATGGCCGGCGGATGGCTACCCGACCCACAAGCTGCTTGAGAGCCTGCGCCAGCAGTAAGCATTCCGGGGCCGCTGCGCgg                                                                                                                                             <  2:188863/96‑1 (MQ=255)
                                                      cccAGCAAGGGCTGGGATGGCCGGCGGATGGCTACCCGACCCACAAGCTGCTTGAGAGCCTGCGCCAGCAGTAAGCATTCCGGGGCCGCTGCGCgg                                                                                                                                             >  1:188863/1‑96 (MQ=255)
                                                       ccAGCAAGGGCTGGGATGGCCGGCGGATGGCTACCCGACCCACAAGCTGCTTGAGAGCCTGCGCCAGCAGTAAGCATTCCGGGGCCGCTGCGCGGCCCCGGTAATCTCACGCCCTGAACAGATCCTGCTCCAGCACCAGTGTTTGCTg                                                                                        >  7:209543/1‑148 (MQ=255)
                                                       ccAGCAAGGGCTGGGATGGCCGGCGGATGGCTACCCGACCCACAAGCTGCTTGAGAGCCTGCGCCAGCAGTAAGCATTCCGGGGCCGCGCAGCGGCCCCggaa                                                                                                                                     <  6:311709/103‑3 (MQ=255)
                                                       ccAGCAAGGGCTGGGATGGCCGGCGGATGGCTACCCGACCCACAAGCTGCTTGAGAGCCTGCGCCAGCAGTAAGCATTCCGGGGCCGCGCAGCGGCCCCggaa                                                                                                                                     >  5:311709/1‑101 (MQ=255)
                                                        cAGCAAGGGCTGGGATGGCCGGCGGATGGCTACCCGACCCACAAGCTGCTTGAGAGCCTGCGCCAGCAGTAAGCATTCCGTGGCCGCTGCGCGGCCCCGGTAATCTCACGCCCTGAACAGATCCTGCTCCAGCACCAGTGTTTGCTGaa                                                                                      <  6:73614/149‑1 (MQ=255)
                                                            aaGGGCTGGGATGGCCGGCGGATGGCTACCCGACCCACAAGCTGCTTGAGAGCCTGCGCCAGCAGTAAGCATTCCGGGGCCGCTGCGCGGCCCCGGTAATCTCACGCCCTGAACAGATCCTGCTCCAGCACCAGTGTTTGCTGAACACt                                                                                  <  2:299410/149‑1 (MQ=255)
                                                                    ggATGGCCGGCGGATGGCTACCCGACCCACAAGCTGCTTGAGAGCCTGCGCCAGCAGTAAGCATTCCGGGGCCGCTGCGCGGCCCCGGTAATCTCACGCCCTGAACAGATCCTGCTCCAGCACCAGTGTTTGCTGAACACTGTCCAGc                                                                           <  4:47958/148‑1 (MQ=255)
                                                                          ccGGCGGATGGCTACCCGACCCACAAGCTGCTTGAGAGCCTGCGCCAGCAGTAAGCATTCCGGGGCCGCTGCGCGGCCCCGGTAATCTCACGCCCTGAACAGATCCTGCTCCAGCACCAGTGTTTGCTGAACACTGTCCAGCCTCAccc                                                                    >  7:322291/1‑149 (MQ=255)
                                                                               ggATGGCTACCCGACCCACAAGCTGCTTGTGAGCCTGCGCCAGCAGTAAGCATTCCGGGGCCGCTGCGCGGCCCCGGTAATCTCACGCCCTGAACAGATCCTGCTCCAGCACCAGTGTTTGCTGAACACTGTCCAGCCTCACCCGAGcc                                                               >  4:33006/1‑149 (MQ=255)
                                                                                           gACCCACAAGCTGCTTGAGAGCCTGCGCCAGCAGTAAGCATTCCGGGGCCGCTGCGCGGCCCCGGTAATCTCACGCCCTGAACAGATCCTGCTCCAGCACCAGTGTTTGCTGAACACTGTCCAGCCTCACCCGAGCCCCCAGGGGCAAg                                                   >  8:303528/1‑149 (MQ=255)
                                                                                           gACCCACAAGCTGCTTGAGAGCCTGCGCCAGCAGTAAGCATTCCGGGGCCGCTGCGCGGCCCCGGTAATCTCACGCCCTGAACAGATCCTGCTCCAGCACCAGTGTTTGCTGAACACTGTCCAGCCTCACCCGAGCCCCCAGGGGCAAg                                                   >  6:107366/1‑149 (MQ=255)
                                                                                            aCCCACAAGCTGCTTGAGAGCCTGCGCCAGCAGTAAGCATTCCGGGGCCGCTGCGCGGCCCCGGTAATCTCACGCCCTGAACAGATCCTGCTCCAGCACCAGTGTTTGCTGAACACTGTCCAGCCTCACCCGAGCCCCCAGGGGCAAGc                                                  <  6:96264/149‑1 (MQ=255)
                                                                                              ccACAAGCTGCTTGAGAGCCTGCGCCAGCAGTAAGCATTCCGGGGCCGCTGCGCGGCCCCGGTAATCTCACGCCCTGAACAGATCCTGCTCCAGCACCAGTGTTTGCTGAACACTGTCCAGCCTCACCCGAGCCCCCAGGGGCAAGCa                                                 >  8:285727/1‑148 (MQ=255)
                                                                                              ccACAAGCTGCTTGAGAGCCTGCGCCAGCAGTAAGCATTCCGGGGCCGCTGCGCGGCCCCGGTAATCTCACGCCCTGAACAGATCCTGCTCCAGCACCAGTGTTTGCTGAACACTGTCCAGCCTCACCCGAGCCCCCAGGGGCAAGCAt                                                <  7:303528/149‑1 (MQ=255)
                                                                                                acaAGCTGCTTGAGAGCCTGCGCCAGCAGTAAGCATTCCGGGGCCGCTGCGCGGCCCCGGTAATCTCACGCCCTGAACAGATCCTGCTCCAGCACCAGTGTTTGCTGAACACTGTCCAGCCTCACCCGAGCCCCCAGGGGCAAGCATa                                               >  5:140161/1‑148 (MQ=255)
                                                                                                 caAGCTGCTTGAGAGCCTGCGCCAGCAGTAAGCATTCCGGGGCCGCTGCGCGGCCCCGGTAATCTCACGCCCTGAACAGATCCTGCTCCAGCACCAGTGTTTGCTGAACACTGTCCAGCCTCACCCGAGCCCCCAGGGGCAAGCATACa                                             >  2:116142/1‑149 (MQ=255)
                                                                                                   aGCTTCTTGAGAGTCTGCGACAGCAGTAAGCATTCCGGGGCCGCTGCGCGGCCTCGGTAATCTCACTGCCTGAAGAGATCCTGCTCGAGCTCCATTGTTTGCTGAACACTGTCGAGCCTCACCCGAGCCCCAATGGGCAAGCATACAtt                                           <  8:322291/149‑1 (MQ=255)
                                                                                                    gctgctTGAGAGCCTGCGCCAGCAGTAAGCATTCCGGGGCCGCTGCGCGGCCCCGGTAATCTCACGCCCTGAACAGATCCTGCTCCAGCACCAGTGTTTGCTGAACACTGTCCAGCCTCACCCGAGCCCCCAGGGGCAAGCATACATTc                                          <  6:140161/149‑1 (MQ=255)
                                                                                                        ctTGAGAGCCTGCGCCAGCAGTAAGCATTCCGGGGCCGCTGCGCGGCCCCGGTAATCTCACGCCCTGAACAGATCCTGCTCCAGCACCAGTGTTTGCTGAACACTGTCCAGCCTCACCCGAGCCCCCAGGGGCAAGCATACATTc                                          <  3:18058/145‑1 (MQ=255)
                                                                                                        ctTGAGAGCCTGCGCCAGCAGTAAGCATTCCGGGGCCGCTGCGCGGCCCCGGTAATCTCACGCCCTGAACAGATCCTGCTCCAGCACCAGTGTTTGCTGAACACTGTCCAGCCTCACCCGAGCCCCCAGGGGCAAGCATACATTCggg                                       <  7:285727/148‑1 (MQ=255)
                                                                                                        ctTGAGAGCCTGCGCCAGCAGTAAGCATTCCGGGGCCGCTGCGCGGCCCCGGTAATCTCACGCCCTGAACAGATCCTGCTCCAGCACCAGTGTTTGCTGAACACTGTCCAGCCTCACCCGAGCCCCCAGGGGCAAGCATACATTCgg                                        <  8:209543/147‑1 (MQ=255)
                                                                                                        ctTGAGAGCCTGCGCCAGCAGTAAGCATTCCGGGGCCGCGGCGCGGCCCCGGGAATATCACGCCCGGAACAGAGACTGCGGCAGCACCAGTGTTTGCTGAACACAGTACAGCCTCACCCCAGCCCCCAGGGGCAAGCATACATTc                                          >  4:18058/1‑145 (MQ=255)
                                                                                                                 cTGCGCCAGCAGTAAGCATTCCGGGGCCGCTGCGCGGCCCCGGTAATCTCACGCCCTGAACAGATCCTGCTCCAGCACCAGTGTTTGCTGAACACTGTCCAGCCTCACCCGAGCCCCCAGGGGCAAGCATACATTCGGGTCACAGTGcc                             >  4:133624/1‑149 (MQ=255)
                                                                                                                  tGCGCCAGCAGTAAGCATTCCGGGGCCGCTGCGCGGCCCCGGTAATCTCACGCCCTGAACAGATCCTGCTCCAGCACCAGTGTTTGCTGAACACTGTCCAGCCTCACCCGAGCCCCCAGGGGCAAGCATACATTCGGGTCACAGTGc                              <  4:71112/147‑1 (MQ=255)
                                                                                                                  tGCGCCAGCAGTAAGCATTCCGGGGCCGCTGCGCGGCCCCGGTAATCTCACGCCCTGAACAGATCCTGCTCCAGCACCAGTGTTTGCTGAACACTGTCCAGCCTCACCCGAGCCCCCAGGGGCAAGCATACATTCGGGTCACAGTGc                              >  3:71112/1‑147 (MQ=255)
                                                                                                                       cagcagTAAGCATTCCGGGGCCGCTGCGCGGCCCCGGTAATCTCACGCCCTGAACAGATCCTGCTCCAGCACCAGTGTTTGCTGAACACTGTCCAGCCTCACCCGAGCCCCCAGGGGCAAGCATACATTCGGGTCACAGTGCCCGCTgc                       >  8:271018/1‑149 (MQ=255)
                                                                                                                         gcagTAAGCATTCCGGGGCCGCTGCGCGGCCCCGGTAATCTCACGCCCTGAACACATCCTGCTCCAGCACCAGTGTTTGCTGAACACTGTCCAGCCTCACCCGAGCCCCCAGGGGCAAGCATACATTCGGGTCACAGTTCCCGCTgcgc                     <  8:271212/149‑1 (MQ=255)
                                                                                                                            gTAAGCATTCCGGGGCCGCTGCGCGGCCCCGGTAATCTCACGCCCTGAACAGATCCTGCTCCAGCACCAGTGTTTGCTGAACACTGTCCAGCCTCACCCGAGCCCCCAGGGGCAAGCATACATTCGGGTCACAGTGCCCGCTGCGCCaa                  <  3:133624/149‑1 (MQ=255)
                                                                                                                             tAAGCATTCCGGGGCCGCTGCGCGGCCCCGGTAATCTCACGCCCTGAACAGATCCTGCTCCAGCACCAGTGTTTGCt                                                                                         <  8:81142/77‑1 (MQ=255)
                                                                                                                             tAAGCATTCCGGGGCCGCTGCGCGGCCCCGGTAATCTCACGCCCTGAACAGATCCTGCTCCAGCACCAGTGTTTGCt                                                                                         >  7:81142/1‑77 (MQ=255)
                                                                                                                             tAAGCATTCCGGGGCCGCTGCGCGGCCCCGGTAATCTCACGCCCTGAACAGATCCTGCTCCAGCACCAGTGTTTGCTGAACACTGTCCAGCCTCACCCGAGCCCCCAGGGGCAAGCATACATTCGGGTcaca                                  >  3:47652/1‑132 (MQ=255)
                                                                                                                             tAAGCATTCCGGGGCCGCTGCGCGGCCCCGGTAATCTCACGCCCTGAACAGATCCTGCTCCAGCACCAGTGTTTGCTGAACACTGTCCAGCCTCACCCGAGCCCCCAGGGGCAAGCATACATTCGGGTcaca                                  <  4:47652/132‑1 (MQ=255)
                                                                                                                                  aTTCCGGGGCCGCTGCGCGGCCCCGGTAATCTCACGCCCTGAACAGATCCTGCTCCAGCACCAGTGTTTGCTGAACACTGTCCAGCCTCACCCGAGCCCCCAGGGGCAAGCATACATTCGGGTCACAGTGCCCGCTGCGCCAACCCGc             >  1:43754/1‑148 (MQ=255)
                                                                                                                                   ttCCGGGGCCGCTGCGCGGCCCCGGTAATCTCACGCCCTGAACAGATCCTGCTCCAGCACCAGTGTTTGCTGAACACTGTCCAGCCTCACCCGAGCCCCCAGGGGCAAGCATACATTCGGGTCACAGTGCCCGCTGCGCCAACCCGCCa           <  8:262285/149‑1 (MQ=255)
                                                                                                                                   taccGGGGCCGCGCAGCGGCCCCGGTAATCTCACGCCCTGAACAGATCCTGCTCCAGCACCAGTGTTAGCTGAACACTGTCCAGCCTCACCCGAGCCCCCAGGGGCAAGCATACATTCGGGTCACAGTGCCCGCTGCGCCAAccc               <  7:334037/143‑1 (MQ=255)
                                                                                                                                   taccGGGGCCGCGCAGCGGCCCCGGTAATCTCACGCCCTGAACAGATCCTGCTCCAGCACCAGTGTTAGCTGAACACTGTCCAGCCTCACCCGAGCCCCCAGGGGCAAGCATACATTCGGGTCACAGTGCCCGCTGCGCCAAccc               >  8:334037/3‑145 (MQ=255)
                                                                                                                                     ccGGGGCCGCGCAGCGGCCCCGGTAATCTCACGCCCTGAACAGATCCTGCTCCAGCACCAGTGTTTGCGGAACACTGGCCAGCCTCCCCCGAGCCCCCCGGGGCGAGCAGACATTCGGGTCCCCGTGCCCGCTGCGCGCCCCGGCCAGc         >  4:16325/1‑149 (MQ=255)
                                                                                                                                             gCTGCGCGGCCCCGGTAATCTCACGCCCTGAACAGATCCTGCTCCAGCACCAGTGTTTGCTGAACACTGTCCAGCCTCACCCGAGCCCCCAGGGGCAAGCATACATTCGGGTCACAGTGCCCGCTGCGCCAACCCGCCAGCACCGGca  >  8:193669/1‑148 (MQ=255)
                                                                                                                                             gCTGCGCGGCCCCGGTAATCTCACGCCCTGAACAGATCCTGCTCCAGCACCAGTGTTTGCTGAACACTGTCCAGCCTCACCCGAGCCCCCAGGGGCAAGCATACATTCGGGTCACAGTGCCCGCTGCGCCAACCCGCCAGCACCGGca  <  7:193669/148‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                                 
CGGGTAATGCCGATGGCATTATCGGGGCCAATACCCGCAAGGCAATCCGCAATGCCCAGCAAGGGCTGGGATGGCCGGCGGATGGCTACCCGACCCACAAGCTGCTTGAGAGCCTGCGCCAGCAGTAAGCATTCCGGGGCCGCTGCGCGGCCCCGGTAATCTCACGCCCTGAACAGATCCTGCTCCAGCACCAGTGTTTGCTGAACACTGTCCAGCCTCACCCGAGCCCCCAGGGGCAAGCATACATTCGGGTCACAGTGCCCGCTGCGCCAACCCGCCAGCACCGGCA  >  NC_002947/5462250‑5462538

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑