Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,516,304	A→G	7.2%	I39V (ATC→GTC) ‡	PP_4849 →	molecular chaperone DnaK

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,516,304	0	A	G	7.2%	158.7 / 2.1	55	I39V (ATC→GTC) ‡	PP_4849	molecular chaperone DnaK
Reads supporting (aligned to +/- strand):  ref base A (26/25);  new base G (2/2);  total (28/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

GGCCGTTTTGCCTCCGGAAGCCCATTTCCCATGTCTGACGTATCCCCGGCCCGCGCCCTGGGCATCGACTTTGGCACCTCCAACTCCACAGTCGGTTGGCATCGGCCT‑GGCGTCGAGTCGCTGATTGCCCTGGAAGACGGCAAGATCACCTTGCCCTCGGTGGTGTTCTTCAATATCGAAGAGCGGCGCCCGGTGTATGGCCGCCTGGCGCTGCACGAGTACCTGGAAGGCTACGAAGGCC‑GCCTGATGCGCTCGCTGAAAAGCCTGCTGGGCTCGAAGCTGATCAAGC  >  NC_002947/5516160‑5516448                                                                                                                                                  |                                                                                                                                                  ggCCGTTTTGCCTCCGGAAGCCCATTTCCCATGTCTGACGTATCCCCGGCCCGCGCCCTGGGCATCGACTTTGGCACCTCCAACTCCACAGTCGGTTGGCATCGGCCT‑GGCGTCGAGTCGCTGATTGCCCTGGAAGACGGCAAGATCAc                                                                                                                                               >  2:271359/1‑149 (MQ=255)     gTTTTGCCTCCGGAAGCCCATTTCCCATGTCTGACGTATCCCCGGCCCGCGCCCTGGGCATCGACTTTGGCACCTCCAACTCCACAGTCGGTTGGCATCGGCCT‑GGCGTCGAGTCGCTGATTGCCCTGGAAGACGGCAAGATCACCTTg                                                                                                                                           <  3:93769/149‑1 (MQ=255)            cTCCGGAAGCCCATTTCCCATGTCTGACGTATCCCCGGCCCGCGCCCTGGGCATCGACTTTGGCACCTCCAACTCCACAGTCGGTTGGCATCGGCCTCGGCGTCGAGTCGCTGATTGCCCTGGAAGACGGCAAGATCACCTTGCCCTCg                                                                                                                                     <  1:271359/149‑1 (MQ=255)               cGGAAGCCCATTTCCCATGTCTGACGTATCCCCGGCCCGCGCCCTGGGCATCGACTTTGGCACCTCCAACTCCACAGTCGGTTGGCATCGGCCT‑GGCGTCGAGTCGCTGATTGCCCTGGAAGACGGCAAGATCACCTTGCCCTCggtg                                                                                                                                  <  1:19684/148‑1 (MQ=255)               cGGAAGCCCATTTCCCATGTCTGACGTATCCCCGGCCCGCGCCCTGGGCATCGACTTTGGCACCTCCAACTCCACAGTCGGTTGGCATCGGCCT‑GGCGTCGAGTCGCTGATTGCCCTAGAAGACGGCAAGATCACCTTGCCCTCggt                                                                                                                                   <  5:325310/147‑1 (MQ=255)                  aaGACCATTTCCCATGTCTGACGTATGCCGGGCCCGCGCACTTGGCATCGAGTTTGGCACCTCAAACTCCATAGTCGGTTGGCATCGGCCT‑TGCGTCGAGTAGCTGATTGCCCTGGAAGACGGCAAGATCACCTTGCCCTCGGTGgtgt                                                                                                                              <  2:90905/149‑1 (MQ=255)                   aGCCCATTTCCCATGTCTGACGTATCCCCGGCCCGCGCCCTGGGCATCGACTTTGGCACCTCCAACTCCACAGTCGGTTGGCATCGGCCT‑GGCGTCGAGTCGCTGATTGCCCTGGAAGACGGCAAGATCACCTTGCCCTCggtg                                                                                                                                  >  6:225004/1‑144 (MQ=255)                   aGCCCATTTCCCATGTCTGACGTATCCCCGGCCCGCGCCCTGGGCATCGACTTTGGCACCTCCAACTCCACAGTCGGTTGGCATCGGCCT‑GGCGTCGAGTCGCTGATTGCCCTGGAAGACGGCAAGATCACCTTGCCCTCggtg                                                                                                                                  <  5:225004/144‑1 (MQ=255)                      ccATTTCCCATGTCTGACGTATCCCCGGCCCGCGCCCTGGGCATCGACTTTGGCACCTCCAACTCCACAGTCGGTTGGCATCGGCCT‑GGCGTCGAGTCGCTGATTGCCCTGGAAGACGGCAAGATCACCTTGCCCTCGGTGGTGttctt                                                                                                                          >  4:267755/1‑149 (MQ=255)                       cATTTCCCATGTCTGACGTATCCCCGGCCCGCGCCCTGGGCATCGACTTTGGCACCTCCAACTCCACAGTCGGTTGGCATCGGCCT‑GGCGTCGAGTCGCTGATTGCCCTGGAATACGGCAAGATCACCTTGCCCTCGGTGGTGttctt                                                                                                                          >  1:350920/1‑148 (MQ=255)                       cATTTCCCATGTCTGACGTATCCCCGGCCCGCGCCCTGGGCATCGACTTTGGCACCTCCAACTCCACAGTCGGTTGGCATCGGCCT‑GGCGTCGAGTCGCTGATTGCCCAGGAACACGGCAACATCACCTTGCCCTCGGTGGTGatctt                                                                                                                          >  1:356542/1‑148 (MQ=255)                            cccATGTCTGACGTATCCCCGGCCCGCGCCCTGGGCATCGACTTTGGCACCTCCAACTCCACAGTCGGTTGGCATCGGCCT‑GGCGTCGAGTCGCTGATTGCCCTGGAAGACGGCAAGATCACCTTGCCCTCgg                                                                                                                                    >  5:81774/1‑133 (MQ=255)                            cccATGTCTGACGTATCCCCGGCCCGCGCCCTGGGCATCGACTTTGGCACCTCCAACTCCACAGTCGGTTGGCATCGGCCT‑GGCGTCGAGTCGCTGATTGCCCTGGAAGACGGCAAGATCACCTTGCCCTCgg                                                                                                                                    <  6:81774/133‑1 (MQ=255)                                tGTCTGACGTATCCCCGGCCCGCGCCCTGGGCATCGACTTTGGCACCTCCAACTCCACAGTCGGTTGGCATCGGCCT‑GGCGTCGAGTCGCTGATTGCCCTGGAAGACGGCAAGATCACCTTGCCCTCGGTGGTGTTCTTCAATATCGaa                                                                                                                <  6:107296/149‑1 (MQ=255)                                tGTCTGACGTATCCCCGGCCCGCGCCCTGGGCATCGACTTTGGCACCTCCAACTCCACAGTCGGTTGGCATCGGCCT‑GGCGTCGAGTCGCTGATTGCCCTGGAAGACGGCAAGATCACCTTGCCCTCGGTGGTGTTCTTCAATATCGaa                                                                                                                <  1:265171/149‑1 (MQ=255)                                  tCTGACGTATCCCCGGCCCGCGCCCTGGGCATCGACTTTGGCACCTCCAACTCCACAGTCGGTTGGCATCGGCCT‑GGCGTCGAGTCGCTGATTGCCCTGGAAGACGGCAAGATCACCTTGCCCTCGGTGGTGTTCTTCAATATCGaa                                                                                                                >  3:284072/1‑147 (MQ=255)                                    tGACGTATCCCCGGCCCGCGCCCTGGGCATCGACTTTGGCACCTCCAACTCCACAGTCGGTTGGCATCGGCCT‑GGCGTCGAGTCGCTGATTGCCCTGGAAGACGGCAAGATCACCTTGCCCTCGGTGGTGTTCTTCAATATCGAAGAgc                                                                                                            >  3:47894/1‑149 (MQ=255)                                           tcaccGGCCCGCGCCCTGGGCATCGACTTTGGCACCTCCAACTCCACAGTCGGTTGGCATCGGCCT‑GGCGTCGAGTCGCTGATTGCCCTGGAAGACGGCAAGATCAACTTGCCCTCGGTGGTGTTCTTCAATATCGAAGATCTGCGccc                                                                                                     >  4:248903/4‑149 (MQ=255)                                              ccGGCCCGCGCCCTGGGCATCGACTTTGGCACCTCCAACTCCACAGTCGGTTGGCATCGGCCT‑GGCGTCGAGTCGCTGATTGCCCTGGAAGACGGCAAGATCACCTTGCCCTCGGTGGTGTTCTTCAATATCGAAGAGCGGCGCCCGgt                                                                                                  <  4:47894/149‑1 (MQ=255)                                                      cgcCCTGGGCATCGACTTTGGCACCTCCAACTCCACAGTCGGTTGGCATCGGCCT‑GGCGTCGAGTCGCTGATTGCCCTGGAAGACGGCAAGATCACCTTGCCCTCGGTGGTGTTCTTCAATATCGAAGAGCGGCGCCCGGTGTATGgcc                                                                                          <  4:284072/149‑1 (MQ=255)                                                          cTGGGCATCGACTTTGGCACCTCCAACTCCACAGTCGGTTGGCATCGGCCT‑GGCGTCGAGTCGCTGATTGCCCTGGAAGACGGCAAGATCACCTTGCCCTCGGTGGTGTTCTTCAATATCGAAGAGCGGCGCCCGGTGTATGGCCGCCt                                                                                      >  6:55365/1‑149 (MQ=255)                                                             ggCATCGACTTTGGCACCTCCAACTCCACAGTCGGTTGGCATCGGCCT‑GGCGTCGAGTCGCTGATTGCCCTGGAAGACGGCAAGATCACCTTGCCCTCGGTGGTGTTCTTCAATATCGAAGAGCGGCGCCCGGTGTATGGCCGCCTGgc                                                                                   <  3:248903/149‑1 (MQ=255)                                                             ggCATCGACTTTGGCACCTCCAACTCCACAGTCGGTTGGCATCGGCCT‑GGCGTCGAGGCGCTGATTGCCCTGGAAGACGGCAAGATCACCTTGCCCTCGGTGGTGTTCTTCAATATCGAAGAGCGGCGCCCGGTGTATGGCCGCCTgg                                                                                    <  7:26108/148‑1 (MQ=255)                                                                   gACTTTGGCACCTCCAACTCCACAGTCGGTTGGCATCGGCCT‑GGCGTCGAGTCGCTGATTGCCCTGGAAGACGGCAAGATCACCTTGCCCTCGGTGGTGTTCTTCAATATCGAAGAGCGGCGCCCGGTGTATGGCCGCCTGGCGCTGCa                                                                             >  2:266378/1‑149 (MQ=255)                                                                          gCACCTCCAACTCCACAGTCGGTTGGCATCGGCCT‑GGCCTCGAGTCGCTGATTGCCCTGGAAGACGGCAAGATCACCTTGCCCTCGGTGGTGTTCTTCAATATCGAAGAGCGGCGCCCGGTGTATGGCCGCCTGGCGCTGCACGAGTa                                                                       >  2:220389/1‑148 (MQ=255)                                                                           cACCTCCAACTCCACAGTCGGTTGGCATCGGCCT‑GGCGTCGAGTCGCTGATTGCCCTGGAAGACGGCAAGATCACCTTGCCCTCGGTGGTGTTCTTCAATATCGAAGAGCGGCGCCCGGTGTATGGCCGCCTGGCGCTGCACGAGTAcc                                                                     <  3:267755/149‑1 (MQ=255)                                                                               tCCAACTCCACAGTCGGTTGGCATCGGCCT‑GGCGTCGAGTCGCTGATTGCCCTGGAAGACGGCAAGATCACCTTGCCCTCGGTGGTGTTCTTCAATATCGAAGAGCGGCGCCCGgt                                                                                                  >  4:104283/1‑116 (MQ=255)                                                                               tCCAACTCCACAGTCGGTTGGCATCGGCCT‑GGCGTCGAGTCGCTGATTGCCCTGGAAGACGGCAAGATCACCTTGCCCTCGGTGGTGTTCTTCAATATCGAAGAGCGGCGCCCGgt                                                                                                  <  3:104283/116‑1 (MQ=255)                                                                               tCCAACTCCACAGTCGGTTGGCATCGGCCT‑GGCGTCGAGTCGCTGATTGCCCTGGAAGACGGCAAGATCACCTTGCCCTCGGTGGTGTTCTTCAATATCGAAGAGCGGCGCCCGGTGTATGGCCGCCTGGCGCTGCACGAGTACCTgg                                                                  >  1:172283/1‑148 (MQ=255)                                                                               tCCAACTCCACAGTCGGTTGGCATCGGCCT‑GGCGTCGAGTCGCTGATTGCCCTGGAAGACGGCAAGATCACCTTGCCCTCGGTGGTGTTCTTCAATATCGAAGAGCGGCGCCCGGTGTATGGCCGCCTGGCGCTGCACGAGTACCTgg                                                                  >  2:197795/1‑148 (MQ=255)                                                                                 cAACTCCACAGTCGGTTGGCATCGGCCT‑GGCGTCGAGTCGCTGATTGCCCTGGAAGACGGCAAGATCACCTTGCCCTCGGTGGTGTTCTTCAATATCGAAGAGCGGCGCCCGGTGTATg                                                                                             >  4:277672/1‑119 (MQ=255)                                                                                  aaCTCCACAGTCGGTTGGCATCGGCCT‑GGCGTCGAGTCGCTGATTGCCCTGGAAGACGGCAAGATCACCTTGCCCTCGGTGGTGTTCTTCAATATCGAAGAGCGGCGCCCGGTGTATGGCCGCCTGGCGCTGCACGAGTACCTGGAAgg                                                              >  3:53140/1‑149 (MQ=255)                                                                                           gTCGGTTGGCATCGGCCT‑GGCGTCGAGTCGCTGATTGACCTGGAAGACGTCAAGATCAGCTTGCCCTCGGTGGTGTTCTTCAATATCGAAGAGCTGCGCCCGGTGTATGGCCTCCTGGAGCTGCACGAGTACATGGAAGGCTACTAAg                                                      <  2:350920/148‑1 (MQ=255)                                                                                            tCGGTTGGCATCGGCCT‑GGCGTCGAGTCGCTGATTGCCCTGGAAGACGGGAAGATCAGCTTGCCCTCGGTGGTGTTCTTCAATATCGAAGAGCGGCGCCCGGTGTATGGCCGGCTGGCGCTGCACGAGTACCTGGAAGGCTACGatg                                                      <  2:356542/147‑3 (MQ=255)                                                                                                  ggCATCGGCCT‑GGCGTCGAGTCGCTGATTGCCCTGGAAGACGGCAAGATCACCTTGCCCTCGGTGGTGTTCTTCAATATCGAAGAGCGGCGCCCGGTGTATGGCCGCCTGGCGCTGCACGAGTACCTGGAAGGCTACGAAGGCC‑GCCt                                              <  1:197795/148‑1 (MQ=255)                                                                                                    cATCGGCCT‑GGCGTCGAGTCGCTGATTGCCCTGGAAGACGGCAAGATCACCTTGCCCTCGGTGGTGTTCTTCAATATCGAAGAGCGGCGCCCGGTGTATGGCCGCCTGGCGCTGCACGAGTACCTGGAAGGCTACGAAGGCC‑GCCTGAt                                           >  2:162243/1‑149 (MQ=255)                                                                                                          ccT‑GGCGTCGAGTCGCTGATTGCCCTGGAAGACGGCAAGATCACCTTGCCCTCGGTGGTGTTCTTCAATATCGAAGAGCGGCGCCCGGTGTATGGCCGCCTGGCGCt                                                                                <  6:252823/107‑1 (MQ=255)                                                                                                          ccT‑GGCGTCGAGTCGCTGATTGCCCTGGAAGACGGCAAGATCACCTTGCCCTCGGTGGTGTTCTTCAATATAGAAGAGCGGCGCCCGGTGTATGGCCGCCTGGCGCt                                                                                >  5:252823/1‑107 (MQ=255)                                                                                                                  tCGAGTCGCTGATTGCCCTGGAAGACGGCAAGATCACCTTGCCCTCGGTGGTGTTCTTCAATATCGAAGAGCGGCGCCCGGTGTATGGCCGCCTGGCGCTGCACGAGTACCTGGAAGTCTACGAAGGCC‑TCCTGATGCGCTCGCTGaaa                              <  1:220389/149‑1 (MQ=255)                                                                                                                      gTCGCTGATTGCCCTGGAAGACGGCAAGATCACCTTGCCCTCGGTGGTGTTCTTCAATATCGAAGAGCGGCGCCCGGTGTATGGCCGCCTGGCGCTGCACGAGTACCTGGAAGGCTACGAAGGCC‑GCCTGATGCGCTCGCTGAAAAGcc                          <  1:162243/149‑1 (MQ=255)                                                                                                                         gCTGATTGCCCTGGAAGACGGCAAGATCACCTTGCCCTCGGTGGTGTTCTTCAATATCGAAGAGCGGCGCCCGGTGTATGGCCGCCTGGCGCTGCACGAGTACCTGGAAGGCTACGAAGGCC‑GCCTGATGCGCTCGCTGAAAAGCctg                        <  2:172283/148‑1 (MQ=255)                                                                                                                            gATTGCCCTGGAAGACGGCAAGATCACCTTGCCCTCGGTGGTGTTCTTCAATATCGAAGAGCGGCGCCCGGTGTATGGCCGCCTGGCGCTGCACGAGTACCTGGAAGGCTACGAAGGCC‑GCCTGATGCGCTCGCTGAAAAGCCTGCTgg                    >  2:189718/1‑149 (MQ=255)                                                                                                                            gATTGCCCTGGAAGACGGCAAGATCACCTTGCCCTCGGTGGTGTTCTTCAATATCGAAGAGCGGCGCCCGGTGTATGGCCGCCTGGCGCTGCACGAGTACCTGGAAGGCTACGAAGGCC‑GCCTGATGCGCTCGCTGAAAAGCCTGCTgg                    >  1:51403/1‑149 (MQ=255)                                                                                                                                 cccTGGAAGACGGCAAGATCACCTTGCCCTCGGTGGTGTTCTTCAATATCGAAGAGCGGCGCCCGGTGTATGGCCGCCTGGCGCTGCACGAGTACCTGGAAGGCTACGAAGGCC‑GCCTGATGcgctcgc                                   <  3:103648/129‑1 (MQ=255)                                                                                                                                 cccTGGAAGACGGCAAGATCACCTTGCCCTCGGTGGTGTTCTTCAATATCGAAGAGCGGCGCCCGGTGTATGGCCGCCTGGCGCTGCACGAGTACCTGGAAGGCTACGAAGGCC‑GCCTGATGcgctcgc                                   >  4:103648/1‑129 (MQ=255)                                                                                                                                  ccTGGAAGACGGCAAGATCACCTTGCCCTCGGTGGTGTTCTTCAATATCGAAGAGCGGCGCCCGGTGTATGGCCGCCTGGCGCTGCACGAGTACCTGGAAGGCTACGAAGGCC‑GCCTGATGCGCTCGCTGAAAAGCCTGCTGGGCTCg               >  3:148766/1‑148 (MQ=255)                                                                                                                                  ccTGGAAGACGGCAAGATCACCTTGCCCTCGGTGGTGTTCTTCAATATCGAAGAGCGGCGCCCGGTGTATGGCCGCCTGGCGCTGCACGAGTACCTGGAAGGCTACGAAGGCC‑GCCTGATGCGCTCGCTGAAAAGCCTGCTGGGCTCg               >  8:248406/1‑148 (MQ=255)                                                                                                                                         gagggCAAGGTGATCTTGCCCTCGGTGGTGTTCTTCAATATCGAAGAGCGGCGCCCGGTGTATGGCCGCCTGGCGCTGCACGAGTACCTGGAAGGCTACGAAGGCC‑GCCTGATGCGCTCGCTGAAAAGCctgct                      >  3:292281/4‑134 (MQ=255)                                                                                                                                         gagggCAAGGTGATCTTGCCCTCGGTGGTGTTCTTCAATATCGAAGAGCGGCGCCCGGTGTATGGCCGCCTGGCGCTGCACGAGTACCTGGAAGGCTACGAAGGCC‑GCCTGATGCGCTCGCTGAAAAGCctgct                      <  4:292281/131‑1 (MQ=255)                                                                                                                                          agggCAAGGTGATCTTGCCCTCGGTGGTGTTCTTCAATATCGAAGAGCGGCGCCCGGTGTATGGCCGCCTGGCGCTGCACGAGTACCTGGAAGGCTACGAAGGCC‑GCCTGATGCGCTCGCTGAAAAGCCTGCTGGGCTc                <  5:301998/137‑1 (MQ=255)                                                                                                                                          agggCAAGGTGATCTTGCCCTCGGTGGTGTTCTTCAATATCGAAGAGCGGCGCCCGGTGTATGGCCGCCTGGCGCTGCACGAGTACCTGGAAGGCTACGAAGGCC‑GCCTGATGCGCTCGCTGAAAAGCCTGCTGGGCTc                >  6:301998/3‑139 (MQ=255)                                                                                                                                            ggCAAGATCACCTTGCCCTCGGTTGTGTTCTTCAATATCGAAGAGCGGCGCCCGGTGTATGGCCGCCTGGCGCTGCACGAGTACCTGGAAGGCTACGAAGGCC‑GCCTGATGCGCTCGCTGaaaa                             <  5:247195/124‑1 (MQ=255)                                                                                                                                            ggCAAGATCACCTTGCCCTCGGTGGTGTTCTTCAATATCGAAGAGCGGCGCCCGGTGTATGGCCGCCTGGCGCTGCACGAGTACCTGGAAGGCTACGAAGGCC‑GCCTGATGCGCTCGCTGaaaa                             >  6:247195/1‑124 (MQ=255)                                                                                                                                            ggCAAGATCACCTTGCCCTCGGTGGTGTTCTTCAATATCGAAGAGCGGCGCCCGGTGTATGGCCGCCTGGCGCTGCACGAGTACCTGGAAGGCTACGAAGGCC‑GCCTGATGCGCTCGCTGAAAAGCCTGCTGGGCTCGAAGCTGATc      >  1:82275/1‑147 (MQ=255)                                                                                                                                               aaGATCACCTTTCCCTCGGTTGTGTTCTTAAATATCGAATATCGGCGCCCGGTGTATGGCCGCCTGGCGCTTCAAGTGTACCTTGAAGGCTACGAAGGCCAGCCTGATGCGCTCGCTGAAAAGCCTGCTGGGCTCGAAGCTGATCAAGc  <  1:266378/149‑1 (MQ=255)                                                                                                                                                  |                                                                                                                                                  GGCCGTTTTGCCTCCGGAAGCCCATTTCCCATGTCTGACGTATCCCCGGCCCGCGCCCTGGGCATCGACTTTGGCACCTCCAACTCCACAGTCGGTTGGCATCGGCCT‑GGCGTCGAGTCGCTGATTGCCCTGGAAGACGGCAAGATCACCTTGCCCTCGGTGGTGTTCTTCAATATCGAAGAGCGGCGCCCGGTGTATGGCCGCCTGGCGCTGCACGAGTACCTGGAAGGCTACGAAGGCC‑GCCTGATGCGCTCGCTGAAAAGCCTGCTGGGCTCGAAGCTGATCAAGC  >  NC_002947/5516160‑5516448

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑