Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,524,281	C→T	7.7%	V198I (GTC→ATC)	yhjG ←	protein YhjG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,524,281	0	C	T	7.7%	148.3 / 2.9	52	V198I (GTC→ATC)	yhjG	protein YhjG
Reads supporting (aligned to +/- strand):  ref base C (22/26);  new base T (2/2);  total (24/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.23e-01

ACGGCTGGCTGGCGTCCTGCAGGGCCAGCAGGCCACCGATCTTGCCGGTGCCGGACACTGGCTGGCCTTTGTAGCGGCCCTGGGCCTTGAGGCCGAAGGCATAGTCCTGAGCACCGCCGGCCTTCTCGGCGCTGGCCTTGCCGACAATCTCACTGAACGGGATTGGCT‑TGCCAAGCGGGTCGATCTGCACCTTCATGCTGGTTTTCAGGGTCTGGTCGTCGAAGCTGACGTTGCCCTGGTCAAAGCCGATGGCGCCGATGTCCAGCTGCCATTTGGAAGGTTCGGCGTTT  >  NC_002947/5524137‑5524426                                                                                                                                                 |                                                                                                                                                   aCGGCTGGCTGGCGTCCTGCAGGGCCAGCAGGCCACCGATCTTGCCGGTGCCGGACACTGGCTGGCCTTTGTAGCGGCCCTGGGCCTTGAGGCCGAAGGCATAGTCCTGAGCACCGCCGGCCTTCTCGGCGCTGGCCTTGCCGACAAtc                                                                                                                                                <  7:172911/149‑1 (MQ=255)    gctggctgGCGTCCTGCAGGGCCAGCAGGCCACCGATCTTGCCGGTGCCGGACACTGGCTGGCCTTTGTAGCGGCCCTGGGCCTTGAGGCCGAAGGCATAGTCCTGAGCACCGCCGGCCTTCTCGGCGCTGGCCTTGCCGACAATCTCa                                                                                                                                             >  1:312753/1‑149 (MQ=255)         ctggcGTCCTGCAGGGCCAGCAGGCCACCGATCTTGCCGGTGCCGGACACTGGCTGGCCTTTGTAGCGGCCCTGGGCCTTGAGGCCGAAGGCATAGTCCTGAGCACCGCCGGCCTTCTCGGCGCTGGCCTTGCCGACAATCTCACTGaa                                                                                                                                        <  7:200093/149‑1 (MQ=255)            gcGTCCTGCAGGGCCAGCAGGCCACCGATCTTGCCGGTGCCGGACACTGGCTGGCCTTTGTAGCGGCCCTGGGCCTTGAGGCCGAAGGCATAGTCCTGAGCACCGCCGGCCTTCTCGGCGCTGGCCTTGCCGACAATCTCACTGAACg                                                                                                                                      <  1:2638/148‑1 (MQ=255)                        gCCAGCAGGCCACCGATCTTGCCGGTGCCGGACACTGGCTGGCCTTTGTAGCGGCCCTGGGCCTTGAGGCCGAAGGCATAGTCCTGAGCACCGCCGGCCTTCTCGGCGCTGGCCTTGCCGACAATCTCACTGAACGGGATTGGCT‑TGc                                                                                                                         >  7:171074/1‑148 (MQ=255)                           agcagGCCACCGATCTTGCCGGTGCCGGACACTGGCTGGCCTTTGTAGCGGCCCTGGGCCTTGAGGCCGAAGGCATAGTCCTGAGCACCGCCGGCCTTCTCGGCGCTGGCCTTGCCGACAATCTCACTGAACg                                                                                                                                      >  3:174488/1‑133 (MQ=255)                           agcagGCCACCGATCTTGCCGGTGCCGGACACTGGCTGGCCTTTGTAGCGGCCCTGGGCCTTGAGGCCGAAGGCATAGTCCTGAGCACCGCCGGCCTTCTCGGCGCTGGCCTTGCCGACAATCTCACTGAACg                                                                                                                                      <  4:174488/133‑1 (MQ=255)                               ggCCACCGATCTTGCCGGTGCCGGACACTGGCTGGCCTTTGTAGCGGCCCTGGGCCTTGAGGCCGAAGGCATAGTCCTGAGCACCGCCGGCCTTCTCGGCGCTGGCCTTGACGACAATCTCACTGAACGGGATTGGCT‑TGCCAAGCggg                                                                                                                 <  5:92630/149‑1 (MQ=255)                                  cACCGATCTTGCCGGTGCCGGACACTGGCTGGCCTTTGTAGCGGCCCTGGGCCTTGAGGCCGAAGGCATAGTCCTGAGCACCGCCGGCCTTCTCGGCGCTGGCCTTGCCGATAATCTCACTGAACGGGATTGGCT‑TGCCAAGCggg                                                                                                                 >  8:97449/1‑146 (MQ=255)                                  cACCGATCTTGCCGGTGCCGGACACTGGCTGGCCTTTGTAGCGGCCCTGGGCCTTGAGGCCGAAGGCATAGTCCTGAGCACCGCCGGCCTTCTCGGCGCTGGCCTTGCCGATAATCTCACTGAACGGGATTGGCT‑TGCCAAGCggg                                                                                                                 >  8:92242/1‑146 (MQ=255)                                  cACCGATCTTGCCGGTGCCGGACACTGGCTGGCCTTTGTAGCGGCCCTGGGCCTTGAGGCCGAAGGCATAGTCCTGAGCACCGCCGGCCTTCTCGGCGCTGGCCTTGCCGATAATCTCACTGAACGGGATTGGCT‑TGCCAAGCggg                                                                                                                 <  7:97449/146‑1 (MQ=255)                                  cACCGATCTTGCCGGTGCCGGACACTGGCTGGCCTTTGTAGCGGCCCTGGGCCTTGAGGCCGAAGGCATAGTCCTGAGCACCGCCGGCCTTCTCGGCGCTGGCCTTGCCGATAATCTCACTGAACGGGATTGGCT‑TGCCAAGCggg                                                                                                                 <  7:92242/146‑1 (MQ=255)                                        atctgccggtgccggACACTGGCTGGCCTTGGTAGCGGCCCTGGGCCTTGAGGCCGAAGGCATAGTCCTGAGCACCGCCGGCCTTCTCGGCGCTGGCCTTGCCGACAATCTCACTGAACGCGATTGGCT‑TGCCAAGCGGGTCGATCTGc                                                                                                        <  7:179116/146‑1 (MQ=255)                                            gccggtgccggACACTGGCTGGCCTTTGTAGCGGCCCTGGGCCTTGAGGCCGAAGGCATAGTCCTGAGCACCGCCGGCCTTCTCGGCGCTGGCCTTGCCGACAATCTCACTGAAC‑GGATTGGCT‑TGCCAAGCGGGTCGATCTGCACCt                                                                                                    <  3:71680/148‑1 (MQ=255)                                             ccggtgccggACACTGGCTGGCCTTTGTAGCGGCCCTGGGCCTTGAGGCCGAAGGCATAGTCCTGAGCACCGCCGGCCTTCTCGGCGCTGGCCTTGCCGACAATCTCACTGAACGGGATTGGCT‑TGACAAGCGGGTCGATCCGc                                                                                                        >  8:179116/1‑144 (MQ=255)                                                   ccggACACTGGCTGGCCTTTGTAGCGGCCCTGGGCCTTGAGGCCGAAGGCATAGTCCTGAGCACCGCCGGCCTTCTCGGCGCTGGCCTTGCCGACAATCTCACTGAACGGGATTGGCT‑TGCCAAGCGGGTCGATCTGCACCTTCATGc                                                                                              <  2:210849/148‑1 (MQ=255)                                                    cggACACTGGCTGGCCTTTGTAGCGGCCCTGGGCCTTGAGGCCGAAGGCATAGTCCTGAGCACCGCCGGCCTTCTCGGCGCTGGCCTTGCCGACAATCTCACTGAACGGGATTGGCT‑TGCCAAGCGGGTCGATCTGCACCTTCATGCTg                                                                                            <  3:95264/149‑1 (MQ=255)                                                    cggACACTGGCTGGCCTTTGTAGCGGCCCTGGGCCTTGAGGCCGAAGGCATAGTCCTGAGCACCGCCGGCCTTCTCGGCGCTGGCCTTGCCGACAATCTCACTGAACGGGATTGGCT‑TGCCAAGCGGGTCGATCTGCACCTTCATGCTg                                                                                            >  8:273140/1‑149 (MQ=255)                                                           tggctggcCTTTGTAGCGGCCCTGGGCCTTGAGGCCGAAGGCATAGTCCTGAGCACCGCCGGCCTTCTCGGCGCTGGCCTTGCCGACAATCTCACTGAACGGGATTGGCT‑TGCCAAGCGGGTCGATCTGCACCTTCATGCTGGtttt                                                                                       >  5:325403/1‑147 (MQ=255)                                                             gctggcCTTTGTAGCGGCCCTGGGCCTTGAGGCCGAAGGCATAGTCCTGAGCACCGCCGGCCTTCTCGGCGCTGGCCTTGCCGACAATCTCACTGAACGGGATTGGCT‑TGCCAAGCGGGTCGATCTGCACCTTCATGCTGGTTTTCAgg                                                                                   <  5:343982/149‑1 (MQ=255)                                                                 gcCTTTGTAGCGGCCCTGGGCCTTGAGGCCGAAGGCATAGTCCTGAGCACCGCCGGCCTTCTCGGCGCTGGCCTTGCCGACAATCTCACTGAACGGGATTGGCTCTGCCAAGCGGGTCGATCTGCACCTTCATGCTGGTTTTCAGGGTc                                                                                <  1:74260/149‑1 (MQ=255)                                                                   cTTTGTAGCGGCCCTGGGCCTTGAGGCCGAAGGCATAGTCCTGAGCACCGCCGGCCTTCTCGGCGCTGGCCTTGCCGACAATCTCACTGAACGGGATTGGCT‑TGCCAAGCGGGTCGATCTGCACCTTCATGCTGGTTTTCAGGGTCTgg                                                                             <  6:203076/149‑1 (MQ=255)                                                                    tttGTAGCGGCCCTGGGCCTTGAGGCCGAAGGCATAGTCCTGAGCACCGCCGGCCTTCTCGGCGCTGGCCTTGCCGACAATCTCACTGACCGGGATTGGCT‑TGc                                                                                                                         >  2:123576/1‑104 (MQ=255)                                                                      tGTAGCGGCCCTGGGCCTTGAGGCCGAAGGCATAGTCCTGAGCACCGCCGGCCTTCTCGGCGCTGGCCTTGCCGACAATCTCACTGAAGGGGATTGGCT‑TGCCAAGCTGGTCGATCTGCACCTTCATGCTGGTTTTCAGGGTCTGgtc                                                                           <  6:325403/148‑1 (MQ=255)                                                                          gCGGCCCTGGGCCTTGAGGCCGCAGGCTTAGTCCTGAGCACCGACGGCCTTCTCCGCGCTGGCCTTGCCGACAATCTCACTGAACGGGAATGGCT‑TGCCAAGCGGGACGAGATGCACATTCATGCTGGTTGTCAGGGTCTGGtcgtcg                                                                       >  4:277505/1‑148 (MQ=255)                                                                          gCGGCCCTGGGCCTTGAGGCCGAAGGCATAGTCCTGAGCACCGCCGGCCTTCTCGGCGCTGGCCTTGCCGACAATCTCACTGAACGGGAtt                                                                                                                                 <  7:95610/91‑1 (MQ=255)                                                                          gCGGCCCTGGGCCTTGAGGCCGAAGGCATAGTCCTGAGCACCGCCGGCCTTCTCGGCGCTGGCCTTGCCGACAATCTCACTGAACGGGAtt                                                                                                                                 >  8:95610/1‑91 (MQ=255)                                                                          gCGGCCCTGGGCCTTGAGGCCGAAGGCATAGTCCTGAGCACCGCCGGCCTTCTCGGCGCTGGCCTTGCCGACAATCTCACTGAACGGGATTGGCT‑TGcc                                                                                                                        >  2:217004/1‑99 (MQ=255)                                                                           cGGCCCTGGGCCTTGAGGCCGAAGGCATAGTCCTGAGCACCGCCGGCCTTCTCGGCGCTGGCCTTGCCGACAATCTCACTGAACGGGATTGGCT‑TGCCAAGCGGGTCGATCTGCACCTTCATGCTGGTTTTCAGGGTCTGGtcgtcg                                                                       >  8:9453/1‑147 (MQ=255)                                                                             gCCCTGGGCCTTGAGGCCGAAGGCATAGTCCTGAGCACCGCCGGCCTTCTCGGCGCTGGCCTTGCCGACAATCTCACTGAACGGGATTGGCT‑TGCCAAGCGGGTCGATCTGCACCTTCATGCTGGTTTTCAGGGTCTGGTCGTCGAAGc                                                                   >  6:130663/1‑149 (MQ=255)                                                                                     ccTTGAGGCCGAAGGCATAGTCCTGAGCACCGCCGGCCTTCTCGGCGCTGGCCTTGCCGACAATCTCGCTGAACGGGATTGGCT‑TGCCAAGCGGGTCGATCTGCACCTTCATGCTGGTTTTCAGGGTCTGGTCGTCGAAGCTGACGTTg                                                           >  3:79166/1‑149 (MQ=255)                                                                                     ccTTGAGGCCGAAGGCATAGTCCTGAGCACCGCCGGCCTTCTCGGCGCTGGCCTTGCCGACAATCTCACTGAACGGGATTGGCT‑TGCCAAGCGGGTCGATCTGCACCTTCATGCTGGTTTTCAGGGTCTGGTCGTCGAAGCTGACGTTg                                                           >  2:232611/1‑149 (MQ=255)                                                                                         gAGGCCGAAGGCATAGTCCTGAGCACCGCCGGCCTTCTCGGCGCTGGCCTTGCCGACAATCTCACTGAACGGGATTGGCT‑TGCCAAGCGGGt                                                                                                                <  3:324875/92‑1 (MQ=255)                                                                                         gAGGCCGAAGGCATAGTCCTGAGCACCGCCGGCCTTCTCGGCGCTGGCCTTGCCGACAATCTCACTGAACGGGATTGGCT‑TGCCAAGCGGGt                                                                                                                >  4:324875/1‑92 (MQ=255)                                                                                         gAGGCCGAAGGCATAGTCCTGAGCACCGCCGGCCTTCTCGGCGCTGGCCTTGCCGACAATCTCACTGAACGGGATTGGCT‑TGCCAAGCGGGTCGATCTGCACCTTCATGCTGGTTTTCAGGGTCTGGTCGTCGAAGCTGACGTTGccc                                                        <  7:273140/148‑1 (MQ=255)                                                                                           ggCCGAAGGCATAGTCCTGAGCACCGCCTGCCTTCTCGGCGCTGGCCTTGCCGACAATCTCACTGAACGGGTTTGGCT‑TGCCAAGCGGGTCGATCTGCACCGTCATGCTGGTTTTCAgtg                                                                                  <  6:277347/120‑3 (MQ=255)                                                                                           ggCCGAAGGCATAGTCCTGAGCACCGACGGACTTCTCGGCGCTGGCCTTGCCGACAATATCACTGAACGGGATTGGCT‑TGCCAAGCGGGTCGATATGCACCTTCATGCTGGTTTTCAggg                                                                                  >  5:277347/1‑120 (MQ=255)                                                                                             ccGAAGGCATAGTCCTGAGCACCGCCGGCCTTCTCGGCGCTGGCCTTGCCGACAATCTCACTGAACGGGATTGGCT‑TGCCAAGCGGGTCGATCTGCACCTTCATGCTGGTTTTCAGGGTCTGGTCGTCGAAGCTGACGTTGCCCTGGTc                                                   <  2:312753/149‑1 (MQ=255)                                                                                               gAAGGCATAGTCCTGAGCACCGCCGGCCTTCTCGGCGCTGGCCTTGCCGACAATCTCACTGAACGGGATTGGCT‑TGCCAAGCGGGTCGATCTGCACCTTCATGCTGGTTTTCAGGGTCTGGTCGTCGAAGCTGACGTTGCCCTGGTCa                                                  >  8:175876/1‑148 (MQ=255)                                                                                                  ggCATAGTCCTGAGCACCGCCGGCCTTCTCGGCGCTGGCCTTGCCGACAATCTCACTGAACGGGATTGGCT‑TGCCAAGCGGGTCGATCTGCACCTTCATGCTGGTTTTCAGGGTCTGGTCGTCGAAGCTGACGTTGCCCTGGTCAAAGc                                              <  1:232611/149‑1 (MQ=255)                                                                                                     aTAGTCCTGAGCACCGCCGGCCTTCTCGGCGCTGGCCTTGCCGACAATCTCACTGAACGGGATTGGCT‑TGCCAAGCGGGTCGATCTGCACCTTCATGCTGGTTTTCAGGGTCTGGTCGTCGAAGCTGACGTTGCCCTGGTCAAAGCCGa                                           >  3:185882/1‑149 (MQ=255)                                                                                                              aGCACCGCCGGCCTTCTCGGCGCTGGCCTTGCCGACAATCTCACTGAACGGGATTGGCT‑TGCCAAGCGGGTCGATCTGCACCTTCATGCTGGTTTTCAGGGTCTGGTCGTCGAAGCTGACGTTGCCCTGGTCAAAGCCGATGGCGCCg                                   <  7:9453/148‑1 (MQ=255)                                                                                                                      cgGCCTTCTCGGCGCTGGCCTTGCCGACAATCTCACTGAACGGGATTGGCT‑TGCCAAGCGGGTCGATCTGCACCTTCATGCTGGTTTTCAGGGTCTGGTCGTCGAAGCTGACGTTGCCCTGGTCAAAGCCGATGGCGCCGATGTCCAGc                          >  5:125346/1‑149 (MQ=255)                                                                                                                             ctcGGCGCTGGCCTTGCCGACAATCTCACTGAACGGGATTGGCT‑TGCCAAGCGGGTCGATCTGCACCTTCATGCTGGTTTTCAGGGTCTGGTCGTCGAAGCTGACGTTGCCCTGGTCAAAGCCGATGGCGCCGATGTCCAGCTGCCAtt                   >  2:48645/1‑149 (MQ=255)                                                                                                                                ggCGCTGGCCTTGCCGACAATCTCGCTGAACGGGATTGGCT‑TGCCAAGCGGGTCGATCTGCACCTTCATGCTGGTTTTCAGGGTCTGGTCGTCGAAGCTGACGTTGCCCTGGTCAAAGCCGATGGCGCCGATGTCCAGCTGCCATTTg                 <  4:79166/148‑1 (MQ=255)                                                                                                                                 gcgcTGGCCTTGCCGACAATCTCACTGAACGGGATTGGCT‑TGCCAAGCGGGTCGATCTGCACCTTCATGCTGGTTTTCAGGGTCTGGTCGTCGAAGCTGACGTTGCCCTGGTCAAAGCCGATGGCGCCGATGTCCAGCTGCCATTTGGa               <  5:130663/149‑1 (MQ=255)                                                                                                                                  cgcTGGCCTTGCCGACAATCTCACTGAACGGGATTGGCT‑TGCCAAGCGGGTCGATCTGCACCTTCATGCTGGTTTTCAGGGTCTGGTCGTCGAAGCTGACGTTGCCCTGGTCAAAGCCGATGGCGCCGATGTCCAGCTGCCATTTGGaa              <  8:171074/149‑1 (MQ=255)                                                                                                                                    cTGGCCTTGCCGACAATCTCACTGAACGGGATTGGCT‑TGCCAAGCGGGTCGATCTGCACCTTCATGCTGGTTTTCAGGGTCTGGTCGTCGAAGCTGACGTTGCCCTGGTCAAAGCCGATGGCGCCGATGTCCAGCTGCCATTTGGAAgg            >  5:22945/1‑149 (MQ=255)                                                                                                                                        ccTTGCCGACAATCTCACTGAACGGGATTGGCT‑TGCCAAGCGGGTCGATCTGCACCTTCATGCTGGTTTTCAGGGTCTGGTCGTCGAAGCTGACGTTGCCCTGGTCAAAGCCGATGGCGCCGATGTCCAGCTGCCATTTGGaa              <  4:310488/143‑1 (MQ=255)                                                                                                                                        ccTTGCCGACAATCTCACTGAACGGGATTGGCT‑TGCCAAGCGGGTCGATCTGCACCTTCATGCTGGTTTTCAGGGTCTGGTCGTCGAAGCTGACGTTGCCCTGGTCAAAGCCGATGGCGCCGATGTCCAGCTGCCATTTGGaa              >  3:310488/1‑143 (MQ=255)                                                                                                                                        ccTTGCCGACAATCTCACTGAACGGGATTGGCT‑TGCCAAGCGGGTCGATCTGCACCTTCATGCTGGTTTTCAGGGTCTGGTCGTCGAAGCTGACGTTGCCCTGGTCAAAGCCGATGGCGCCGATGTCCAGCTGCCATTTGGAAGGTTCg        <  6:125346/149‑1 (MQ=255)                                                                                                                                              cGACAATCTCACTGAACGGGATTGGCT‑TGCCAAGCGGGTCGATCTGCACCTTCATGCTGGTTTTCAGGGTCTGGTCGTCGAAGCTGACGTTGCCCTGGTCAAAGCCGATGGCGCCGATGTCCAGCTGCCATTTGGAAGGTTCGGCGttt  <  4:185882/149‑1 (MQ=255)                                                                                                                                                 |                                                                                                                                                   ACGGCTGGCTGGCGTCCTGCAGGGCCAGCAGGCCACCGATCTTGCCGGTGCCGGACACTGGCTGGCCTTTGTAGCGGCCCTGGGCCTTGAGGCCGAAGGCATAGTCCTGAGCACCGCCGGCCTTCTCGGCGCTGGCCTTGCCGACAATCTCACTGAACGGGATTGGCT‑TGCCAAGCGGGTCGATCTGCACCTTCATGCTGGTTTTCAGGGTCTGGTCGTCGAAGCTGACGTTGCCCTGGTCAAAGCCGATGGCGCCGATGTCCAGCTGCCATTTGGAAGGTTCGGCGTTT  >  NC_002947/5524137‑5524426

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑