Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,559,451 T→C 7.9% Q349R (CAA→CGA)  hisZ ← ATP phosphoribosyltransferase regulatory subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,559,4510TC 7.9% 144.9 / 2.2 52Q349R (CAA→CGA) hisZATP phosphoribosyltransferase regulatory subunit
Reads supporting (aligned to +/- strand):  ref base T (24/23);  new base C (2/2);  total (27/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AACTCACTGGGCCAGCGGCAGAACCTGCCAGCGCCCGTCTTGCTGAATCAATTGCCGATCACAATCCGCCTCGAGAGCAGCACTCAACGGCTGGCCAGGCAGAGCCTGAACCACACGCTGGCCCTCGTTGCGCAACTGGCAGACTTGCTGCCAGAGGGCCGCATCGCCACTGTCCGGCATCCAGATGCCGCCAGTTGGCAATACGACCTCCGCTCGCCCCAGTGTGACCAGGGTCTTCAAATCCGTGGAGAAACCAGTGGCCGGGCGCGCCCGGCCAAAGTCGGCGCCGATAT  >  NC_002947/5559306‑5559598
                                                                                                                                                 |                                                                                                                                                   
aaCTCACTGGGCCAGCGGCAGAACCTGCCAGCGCCCGTCTTGCTGAATCAATTGCCGATCACAATCCGCCTCGAGAGCAGCACTCAACGGCTGGCCAGGCAGAGCCTGAACCACACGCTGGCCCTCGTTGCGCAACTGGCAGACTtgct                                                                                                                                                  <  7:23084/149‑1 (MQ=255)
aaCTCACTGGGCCAGCGGCAGAACCTGCCAGCGCCCGTCTTGCTGAATCAATTGCCGATCACAATCCGCCTCGAGAGCAGCACTCAACGGCTGGCCAGGCAGAGCCTGAACCACACGCTGGCCCTCGTTGCGCAACTGGCAGACTtgct                                                                                                                                                  <  3:143446/149‑1 (MQ=255)
   tCACTGGGCCAGCGGCAGAACCTGCCAGCGCCCGTCTTGCTGAATCAATTGCCGATCACAATCCGCCTCGAGAGCAGCACTCAACGGCTGGCCAGGCAGAGCCTGAACCACACGCTGGCCCTCGTTGCGCAACTGGCAGACTtgctgc                                                                                                                                                >  6:52285/1‑148 (MQ=255)
         ggCCAGCGGCAGAACCTGCCAGCGCCCGTCTTGCTGAATCAATTGCCGATCACAATCCGCCTCGAGAGCAGCACTCAACGGCTGGCCAGGCAGAGCCTGAACCACACGCTGGCCCTCGTTGCGCAACTGGCAGACTTGCTGCCAGAgg                                                                                                                                          >  6:163924/1‑148 (MQ=255)
         ggCCAGCGGCAGAACCTGCCAGCGCCCGTCTTGCTGAATCAATTGCCGATCACAATCCGCCTCGAGAGCAGCACTCAACGGCTGGCCAGGCAGAGCCTGAACCACACGCTGGCCCTCGTTGCGCAACTGGCAGACTTGCTGCCAGAgg                                                                                                                                          <  5:163924/148‑1 (MQ=255)
           ccAGCGGCAGAACCTGCCAGCGCCCGTCTTGCTGAATCAATTGCCGATCACAATCCGCCTCGAGAGCAGCACTCAACGGCTGGCCAGGCAGAGCCTGAACCACACGCTGGCCCTCGTTGCGCAACTGGCAGACTTGCTGCCAGAGGGcc                                                                                                                                       <  5:52285/149‑1 (MQ=255)
            cAGCGGCAGAACCTGCCAGCGCCCGTCTTGCTGAATCAATTGCCGATCACAATCCGCCTCGAGAGCAGCACTCAACGGCTGGCCAGGCAGAGCCTGAACCACACGCTGGCCCTCGTTGCGCAACTGGCAGACTTGCTGCCAGAGGGCCg                                                                                                                                      >  4:16331/1‑149 (MQ=255)
             aGCGGCAGAACCTGCCAGCGCCCGTCTTGCTGAATCAATTGCCGATCACAATCCGCCTCGAGAGCAGCACTCAACGGCTGGCCAGGCAGAGCCTGAACCACACGCTGGCCCTCGTTGCGCAACTGGCAGACTTGCTGCCAGAGGGCCg                                                                                                                                      >  2:299929/1‑148 (MQ=255)
               cGGCAGAACCTGCCAGCGCCCGTCTTGCTGAATCAATTGCCGATCACAATCCGCCTCGAGAGCAGCACTCAACGGCTGGCCAGGCAGAGCCTGAACCACACGCTGGCCCTCGTTGCGCAACTGGCAGACTCGCTGCCAGAGGGCCGCAt                                                                                                                                   >  4:201149/1‑149 (MQ=255)
                ggCAGAACCTGCCAGCGCCCGTCTTGCTGAATCAATTGCCGATCACAATCCGCCTCGAGAGCAGCACTCAACGGCTGGCCAGGCAGAGCCTGAACCACACGCTGGCCCTCGTTGCGCAACTGGCAGACTTGCTGCCAGAGGGcc                                                                                                                                       <  4:316319/144‑1 (MQ=255)
                ggCAGAACCTGCCAGCGCCCGTCTTGCTGAATCAATTGCCGATCACAATCCGCCTCGAGAGCAGCACTCAACGGCTGGCCAGGCAGAGCCTGAACCACACGCTGGCCCTCGTTGCGCAACTGGCAGACTTGCTGCCAGAGGGcc                                                                                                                                       >  3:316319/1‑144 (MQ=255)
                ggCAGAACCTGCCAGCGCCCGTCTTGCTGAATCAATTGCCGATCACAATCCGCCTCGAGAGCAGCACTCAACGGCTGGCCAGGCAGAGCCTGAACCACACGCTGGCCCTCGTTGCGCAACTGGCAGACTCGCTGCCAGAGGGCCGCATc                                                                                                                                  >  1:44678/1‑149 (MQ=255)
                 gCAGAACCTGCCAGCGCCCGTCTTGCTGAATCAATTGCCGATCACAATCCGCCTCGAGAGCAGCACTCAACGGCTGGCCAGGCAGAGCCTGAACCACACGCTGGCCCTCGTTGCGCAACTGGCAGACTTGCTGCCAGAGGGCCGCATCg                                                                                                                                 <  2:267273/149‑1 (MQ=255)
                 gCAGAACCTGCCAGCGCCCGTCTTGCTGAATCAATTGCCGATCACAATCCGCCTCGAGAGCAGCACTCAACGGCTGGCCAGGCAGAGCCTGAACCACACGCTGGCCCTCGTTGCGCAACTGGCAGACTTGCTGCCAGAGGGCCGCATCg                                                                                                                                 >  6:115702/1‑149 (MQ=255)
                    gAACCTGCCAGCGCCCGTCTTGCTGAATCAATTGCCGATCACAATCCGCCTCGAGAGCAGCACTCAACGGCTGGCCAGGCAGAGCCTGAACCACACGCTGGCCCTCGTTGCGCAACTGGCAGACTTGCTGCCAGAGGGCCGCATCGCCa                                                                                                                              <  1:299929/149‑1 (MQ=255)
                     aaCCTGCCAGCGCCCGTCTTGCTGATTCAATTGCCGATCACAATCCGCCTCGAGAGCAGCACTCAACGGCTGGCCAGGCAGAGCCTGAACCACACGCTGGCCCTCGTTGCGCAACTGGCAGACTTGCTGCCAGAGGGCCGCATCGCCAc                                                                                                                             <  6:305511/149‑1 (MQ=255)
                     aaCCTGCAAGCGCCCGTCTTGCTGAATCAATTGCCGATCACAATCCGCCTCCACAGCACCAATAAACCGCTGGCCAGGCAGAGCCAGAACCACACGCTGGCCCTCGTTGCGCAACTCCCAGACAAGCTGCCAGAGCGCCGCATCGacac                                                                                                                             >  5:226150/1‑149 (MQ=255)
                         tGCCAGCGCCCGTCTTGCTGAATCAATTGCCGATCACAATCCGCCTCGAGAGCAGCACTCAACGGCTGGCCAGGCAGAGCCTGAACCACACGCTGGCCCTCGTTGCGCAACTGGCAGACTTGCTGCCAGAGGGCCGCATCGCCACTGTc                                                                                                                         >  2:152536/1‑149 (MQ=255)
                           ccAGCGCCCGTCTTGCTGAATCAATTGCCGATCACAATCCGCCTCGAGAGCAGCACTCAACGGCTGGCCAGGCAGAGCCTGAACCACACGCTGGCCCTCGTTGCGCAACTGGCAGACTTGCTGCCAGAGGGCCGCATCGCCACTGTcc                                                                                                                        <  3:112192/148‑1 (MQ=255)
                           ccAGCGCCCGTCTTGCTGAATCAATTGCCGATCACAATCCGCCTCGAGAGCAGCACTCAACGGCTGGCCAGGCAGAGCCTGAACCACACGCTGGCCCTCGTTGCGCAACTGGCAGACTTGCTGCCAGAGGGCCGCATCGCCACTGTCCg                                                                                                                       <  3:41115/149‑1 (MQ=255)
                              gcgcCCGTCTTGCTGAATCAATTGCCGATCACAATCCGCCTCGAGAGCAGCACTCAACGGCTGGCGAGGCAGAGCCTGAACCACACGCTGGCCCTCGTTGCGCAACTGGCAGACTTGCTGCCAGAGGGCCGCATCGCCACTGTCCggaa                                                                                                                    <  7:234033/149‑3 (MQ=255)
                                  ccGTCTTGCTGAATCAATTGCCGATCACAATCCGCCTCGAGAGCAGCACTCAACGGCTGGCCAGGCAGAGCCTGAACCACACGCTGGCCCTCGTTGCGCAACTGGCAGACTTGCTGCCAGAGGGCCGCATCGCCACTGTCCGGCATCCa                                                                                                                <  1:250995/149‑1 (MQ=255)
                                      cTTGCTGAATCAATTGCCGATCACAATCCGCCTCGAGAGCAGCACTCAACGGCTGGCCAGGCAGAGCCTGAACAACACGCTGGCCCTCGTTGCGCAACTGGCAGACTTGCTGCCAGAGGGCCGCATCGCCACTGTCCGGCATCCAGATg                                                                                                            >  6:131840/1‑149 (MQ=255)
                                         gCTGAATCAATTGCCGATCACAATCCGCCTCGAGAGCAGCACTCAACGGCTGGCCAGGCAGAGCCTGAACCACACGCTGGCCCTCGTTGCGCAACTGGCAGACTTGCTGCCAGAGGGCCGCATCGCCACTGTCCGGCATCCAGATgccg                                                                                                         >  5:8995/1‑149 (MQ=255)
                                         gCTGAATCAATTGCCGATCACAATCCGCCTCGAGAGCAGCACTCAACGGCTGGCCAGGCAGAGCCTGAACCACACGCTGGCCCTCGTTGCGCAACTGGCAGACTTGCTGCCAGAGGGCCGCATCGCCACTGTCCGGCATCCAGATgc                                                                                                           <  7:257895/147‑1 (MQ=255)
                                          cTGTATGAATTGCCGATCTCAATCCTCCTCGATAGCAGCACTCAACGGCTGGCCATGCAGAGCCTGAACCTCACGCTGGCCCTCGTTGAGCAACTGGCAGACTTGCTGCCAGAGGGCCGCATCGCCACTGTCCGCCATCCAGATgccg                                                                                                         <  6:226150/148‑1 (MQ=255)
                                                  aTTGCCGATCACAATCCGCCTCGAGAGCAGCACTCAACGGCTGGCCAGGCAGAGCCTGAACCACACGCTGGCCCTCGTTGCGCAACTGGCAGACTTGCTGCCAGAGGGCCGCATCGCCACTGTCCGGCATCCAGATGCCGCCAGTTgg                                                                                                 >  5:182825/1‑148 (MQ=255)
                                                   ttGCCGATCACAATCCGCCTCGAGAGCAGCACTCAACGGCTGGCCAGGCAGAGCCTGAACCACACGCTGGCCCTCGTTGCGCAACTGGCAGACTTGCTGCCAGAGGGCCGCATCACCACTGTCCGGCATCCAGATGCCGCCAGTTGGc                                                                                                >  6:19843/1‑148 (MQ=255)
                                                         aTCACAATCCGCCTCGAGAGCAGCACTCAACGGCTGGCCAGGCAGAGCCTGAACCACACGCTGGCCCTCGTTGCGCAACTGGCAGACTTGCTGCCAGAGGGCCGCATCGCCACTGTCCGGCATCCAGATGCCGCCAGTTGGCAATACGa                                                                                         <  1:152536/149‑1 (MQ=255)
                                                                      tCGAGAGCAGCACTCAACGGCTGGCCAGGCAGAGCCTGAACCACACGCTGGCCCTCGTTGCGCAACTGGCAGACTTGCTGCCAGAGGGCCGCATCGCCACTGTCCGGCATCCAGATGCCGCCAGTTGGCAATACGACCTCCGCTCGccc                                                                            >  3:25470/1‑149 (MQ=255)
                                                                               gcaCTCAACGGCTGGCCAGGCAGAGCCTGAACCACACGCTGGCCCTCGTTGCGCAACTGGCAGACTCGCTGCCAGAGGGCCGCATCGCCACTGTCCGGCATCCAGATGCCGCCAGTTGGCAATACGACCTCCGCTCGCCCCAGTGTGAc                                                                   <  2:44678/149‑1 (MQ=255)
                                                                                   tCAACGGCTGGCCAGGCAGAGCCTGAACCACACGCTGGCCCTCGTTGCGCAACTGGCAGACTTGCTGCCAGAGGGcc                                                                                                                                       >  7:248476/1‑77 (MQ=255)
                                                                                   tCAACGGCTGGCCAGGCAGAGCCTGAACCACACGCTGGCCCTCGTTGCGCAACTGGCAGACTTGCTGCCAGAGGGcc                                                                                                                                       <  8:248476/77‑1 (MQ=255)
                                                                                   tCAACGGCTGGCCAGGCAGAGCCTGAACCACACGCTGGCCCTCGTTGCGCAACTGGCAGACTTGCTGCCAGAGGGCCGCATCGCCACTGTCCGGCATCCAGATGCCGCCAGTTGGCAATACGACCTCCGCTCGCCCCAGTGTGACCAgg                                                               >  2:30495/1‑149 (MQ=255)
                                                                                     aaCGGCTGGCCAGGCAGAGCCTGAACCACACGCTGGCCCTCGTTGCGCAACTGGCAGACTTGCTGCCAGAGGGCCGCATCGCCACTGTCCGGCATCCAGATGCCGCCAGTTGGCAATACGACCTCCGCTCGCCCCAGTGTGACCAGGGt                                                             >  2:213063/1‑149 (MQ=255)
                                                                                              ccAGGCAGAGCCTGAACCACACGCTGGCCCTCGTTGCGCAACTGGCAGACTTGCTGCCAGAGGGCCGCATCGCCACTGTCCGGCATCCAGATGCCGCCAGTTGGCAATACGACCTCCGCTCGc                                                                              <  3:105351/123‑1 (MQ=255)
                                                                                              ccAGGCAGAGCCTGAACCACACGCTGGCCCTCGTTGCGCAACTGGCAGACTTGCTGCCAGAGGGCCGCATCGCCACTGTCCGGCATCCAGATGCCGCCAGTTGGCAATACGACCTCCGCTCGc                                                                              >  4:105351/1‑123 (MQ=255)
                                                                                              ccAGGCAGAGCCTGAACCACACGCTGGCCCTCGTTGCGCAACTGGCAGACTCGCTGCCAGAGGGCCGCATCGCCACTGTCCGGCATCCAGATGCCGCCAGTTGGCAATACGACCTCCGCTCGCCCCAGTGTGACCAGGGTCTTCAAATc                                                    <  3:201149/149‑1 (MQ=255)
                                                                                                     gagCCTGAACCACACGCTGGCCCTCGTTGCGCAACTGGCAGACTTGCTGCCAGAGGGCCGCATCGCCACTGTCCGGCATCCAGATGCCGCCAGTTgg                                                                                                 <  7:336788/97‑1 (MQ=255)
                                                                                                     gagCCTGAACCACACGCTGGCCCTCGTTGCGCAACTGGCAGACTTGCTGCCAGAGGGCCGCATCGCCACTGTCCGGCATCCAGATGCCGCCAGTTgg                                                                                                 >  8:336788/1‑97 (MQ=255)
                                                                                                            aaCCACACGCTGGCCCTCGTTGCGCAACTGGCAGACTTGCTGCCAGAGGGCCGCATCACCACTGTCCGGCATCCAGATGCCGCCAGTTGGCAATACGACCTCCGCTCGCCCCAGTGTGACCAGGGTCTTCAAATCCGTGGAGAAACCa                                       <  5:19843/148‑1 (MQ=255)
                                                                                                               cacaCGCTGGCCCTCGTTGCGCAACTGGCAGACTTGCTGCCAGAGGGCCGCATCGCCACTGTCCGGCATCCAGATgc                                                                                                           >  4:130143/1‑77 (MQ=255)
                                                                                                               cacaCGCTGGCCCTCGTTGCGCAACTGGCAGACTTGCTGCCAGAGGGCCGCATCGCCACTGTCCGGCATCCAGATgc                                                                                                           <  3:130143/77‑1 (MQ=255)
                                                                                                                 cacGCTGGCCCTCGTTGCGCAACTGGCAGACTTGCTGCCAGAGGGCCGCATCGCCACTGTCCGGCATCCAGATGCCGCCAGTTGGCAATACGACCTCCGCTCGCCCCAGTGTGACCAGGGTCTTCAAATCCGTGGAGAAACCAGTGGcc                                 >  6:152219/1‑149 (MQ=255)
                                                                                                                    gCTGGCCCTCGTTGCGCAACTGGCAGACTTGCTGCCAGAGGGCCGCATCGCCACTGTCCGGCATCCAGATGCCGCCAGTTGGCAATACGACCTCCGCTCGCCCCAGTGTGACCAGGGTCTTCAAATCCGTGGAGAAACCAGTGGCCggg                              >  5:226335/1‑149 (MQ=255)
                                                                                                                    gCTGGCCCTCGTTGCGCAACTGGCAGACTTGCTGCCAGAGGGCCGCATCGCCACTGTCCGGCATCCAGATGCCGCCAGTTGGCAATACGACCTCCGCTCGCCCCAGTGTGACCAGGGTCTTCAAATCCGTGGAGAAACCAGTGGCCggg                              >  8:147787/1‑149 (MQ=255)
                                                                                                                        gCCCTCGTTGCGCAACTGGCAGACTTGCTGCCAGAGGGCCGCATCGCCACTGTCCGGCATCCAGATGCCGCCAGTTGGCAATACGACCTCCGCTCGCCCCAGTGTGACCAGGGTCTTCAAATCCGTGGAGAAACCAGTGGCCGGgcgcg                          >  7:135185/1‑149 (MQ=255)
                                                                                                                         cccTCGTTGCGCAACTGGCAGACTTGCTGCCAGAGGGCCGCATCGCCACTGTCCGGCATCCAGATGCCGCCAGTTGGCAATACGa                                                                                         <  6:233547/85‑1 (MQ=255)
                                                                                                                         cccTCGTTGCGCAACTGGCAGACTTGCTGCCAGAGGGCCGCATCGCCACTGTCCGGCATCCAGATGCCGCCAGTTGGCAATACGACCTCCGCTCGCCCCAGTGTGACCAGGGTCTTCAAATCCGTGGAGAAACCAGTGGCCGGgcgcgc                         <  1:213063/149‑1 (MQ=255)
                                                                                                                              gTTGCGCAACTGGCAGACTTGCTGCCAGAGGGCCGCATCGCCACTGTCCGGCATCCAGATGCCGCCAGTTGGCAATACGACCTCCGCTCGCCCCAGTGTGACCAGGGTCTTCAAATCCGTGGAGAAACCAGTGGCCGGGCGCGCCCg                      <  4:25470/147‑1 (MQ=255)
                                                                                                                              gTTGCGCAACTGGCAGACTTGCTGCCAGAGGGCCGCATCGCCACTGTCCGGCATCCAGATGCCGCCAGTTGGCAATACGACCTCCGCTCGCCCCAGTGTGACCAGGGTCTTCAAATCCGTGGAGAAACCAGTGGCCGGGCGCGCCCGGc                    >  5:57787/1‑149 (MQ=255)
                                                                                                                                  cgcAACTGGCAGACTTGCTGCCAGAGGGCCGCATCGCCACTGTCCGGCATCCAGATGCCGCCAGTTGGCAATACGACCTCCGCTCGCCCCAGTGTGACCAGGGTCTTCAAATCCGTGGAGAAACCAGTGGCCGGGCGCGCCCGGCCact                <  7:147787/149‑3 (MQ=255)
                                                                                                                                  cgcAACTGGCAGACTTGCTGCCAGAGGGCCGCATCGCCACTGTCCGGCATCCAGATGCCGCCAGTTGGCAATACGACCTCCGCTCGCCCCAGTGTGACCAGGGTCTTCAAATCCGTGGAGAAACCAGTGGCCGGGCGCGCCCGGCCaaa                <  5:131840/149‑1 (MQ=255)
                                                                                                                                            aGACTTGCTGCCAGAGGGCCGCATCGCCACTGTCCGGCATCCAGATGCCGCCAGTTGGCAATTCGACCTCCGCTCGCCCCAGTGTGACCAGGGTCTTCAAATCCGTGGAGAAACCAGTGGCCGGGCGCGCCCGGCCAAAGTCGGCGcc       >  4:25506/1‑148 (MQ=255)
                                                                                                                                              aCTTGCTGCCAGAGGGCCGCATCGCCACTGTCCGGCATCCAGATGCCGCCAGTTGGCAATACGACCTCCGCTCGCCCCAGTGTGACCAGGGTCTTCAAATCCGTGGAGAAACCAGTGGCCGGGCGCGCCCGGCCAAAGTCGGCGCCGa     >  6:5255/1‑148 (MQ=255)
                                                                                                                                                ttGCTGCCAGAGGGCCGCATCGCCACTGTCCGGCATCCAGATGCCGCCAGTTGGCAATACGACCTCCGCTCGCCCCAGTGTGACCAGGGTCTTCAAATCCGTGGAGAAACCAGTGGCCGGGCGCGCCCGGCCAAAGTCGGCGCCGatat  >  3:206730/1‑149 (MQ=255)
                                                                                                                                                ttGCTGCCAGAGGGCCGCATCGCCACTGTCCGGCATCCAGATGCCGCCAGTTGGCAATACGACCTCCGCTCGCCCCAGTGTGACCAGGGTCTTCAAATCCGTGGAGAAACCAGTGGCCGGGCGCGCCCGGCCAAAGTCGGCGCCGatat  >  1:252636/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                                   
AACTCACTGGGCCAGCGGCAGAACCTGCCAGCGCCCGTCTTGCTGAATCAATTGCCGATCACAATCCGCCTCGAGAGCAGCACTCAACGGCTGGCCAGGCAGAGCCTGAACCACACGCTGGCCCTCGTTGCGCAACTGGCAGACTTGCTGCCAGAGGGCCGCATCGCCACTGTCCGGCATCCAGATGCCGCCAGTTGGCAATACGACCTCCGCTCGCCCCAGTGTGACCAGGGTCTTCAAATCCGTGGAGAAACCAGTGGCCGGGCGCGCCCGGCCAAAGTCGGCGCCGATAT  >  NC_002947/5559306‑5559598

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑