Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,633,939	T→C	7.0%	C1157R (TGC→CGC)	putA →	proline dehydrogenase/1‑pyrroline‑5‑carboxylate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,633,939	0	T	C	7.0%	163.3 / 2.6	57	C1157R (TGC→CGC)	putA	proline dehydrogenase/1‑pyrroline‑5‑carboxylate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base T (27/26);  new base C (2/2);  total (29/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.76e-01

CGCCCGGCCGACGCCATTGGCCGCCACTTCCAGCAGCAGGACGGCGAAGGCACGCCGGACCGCACCCTGCACGAACAGCTGGTAAAGCCGCTGCACGGCCTGAAGGCCTGGGCCGAGAACAACCAGCTGGCCGACCTGGCCGCGCTGTGCAGCCAGTTC‑GCCAGCCAGTCGCAGAGCGGCATCGCCCGCCTGCTGCCAGGCCCGACCGGCGAGCGCAACAGCTACACCATTCTGCCGCGTGAGCACGTGCTGTGCCTGGCCGACAACGAAACCGACCTGCTGGCGCAGTTCGCCG  >  NC_002947/5633792‑5634086                                                                                                                                                    |                                                                                                                                                     cgcccgGCCGACGCCATTGGCCGCCACTTCCAGCAGCAGGACGGCGAAGGCACGCCGGACCGCACCCTGCACGAACAGCTGGTAAAGCCGCTGCACGGCCTGAAGGCCTGGGCCGAGAACAACCAGCTGGCCGACCTGGCCGCGCtgtg                                                                                                                                                     <  8:18166/149‑1 (MQ=255)   cccgGCCGACGCCATTGGCCGCCACTTCCAGCAGCAGGACGGCGAAGGCACGCCGGACCGCACCCTGCACGAACAGCTGGTAAAGCCGCTGCACGGCCTGAAGGCCTGGGCCGAGAACAACCAGCTGGCCGACCTGGCCGCGCTGTGCa                                                                                                                                                   <  1:129806/149‑1 (MQ=255)             gCCATTGGCCGCCACTTCCAGCAGCAGGACGGCGAAGGCACGCCGGACCGCACCCTGCACGAACAGCTGGTAAAGCCGCTGCACGGCCTGAAGGCCTGGGCCGAGAACAACCAGCTGGCCGACCTGGCCGCGCTGTGCAGCCAGTTC‑gc                                                                                                                                        <  1:141361/149‑1 (MQ=255)             gCCATTGGCCGCCACTTCCAGCAGCAGGACGGCGAAGGCACGCCGGACCGCACCCTGCACGAACAGCTGGTAAAGCCGCTGCACGGCCTGAAGGCCTGGGCCGAGAACAACCAGCTGGCCGACCTGGCCGCGCTGTGCAGCCAGTTC‑gc                                                                                                                                        >  8:35080/1‑149 (MQ=255)               cATTGGCCGCCACTTCCAGCAGCAGGACGGCGAAGGCACGCCGGACCGCACCCTGCACGAACAGCTGGTAAAGCCGCTGCACGGCCTGAAGGCCTGGGCCGAGAACAACCAGCTGGCCGACCTGGCCGCGCTGTGCAGCCAGTTC‑gcca                                                                                                                                      <  6:113435/149‑1 (MQ=255)                            ttCCAGCAGCAGGACGGCGAAGGCACGCCGGACCGCACCCTGCACGAACAGCTGGTAAAGCCGCTGCACGGCCTGAAGGCCTGGGCCGAGAACAACCAGCTGG‑CGAACTGGCTGCACAGCGCGGCCAGGTCGgccagctg                                                                                                                                  <  3:326682/140‑3 (MQ=37)                            ttCCAGCAGCAGGACGGCGAAGGCACGCCGGACCGCACCCTGCACGAACAGCTGGTAAAGCCGCTGCACGGCCTGAAGGCCTGGGCCGAGAACAACCAGCTGG‑CGAACTGGCTGCACAGCGCGGCCAGGTCGgccagctg                                                                                                                                  >  4:326682/1‑138 (MQ=37)                              ccAGCAGCAGGACGGCGAAGGCACGCCGGACCGCACCCTGCCCGAACAGCTGGTAACGCCGCTGCACGGCCTGACGGCCTGGGCCGAGAACAACCAGCTGGCCGACCTGGCCGCGCTGTGACGCCAGTTC‑GCCAGCCAGACGCAGAGCg                                                                                                                       >  3:216379/1‑149 (MQ=255)                                agcagcagGACGGCGAAGGCACGCCGGACCGCACCCTGCACGAACAGCTGGTAAAGCCGCTGCACGGCCTGAAGGCCTGGGCCGAGAACAACCAGCTGGCCGACCTGGCCGCGCTGTGCAGCCAGTTC‑GCCAGCCAGTCGCAGAGCgg                                                                                                                      >  3:217281/1‑148 (MQ=255)                                         aCGGCGAAGGCACGCCGGACCGCACCCTGCACGAACAGCTGGTAAAGCCGCTGCACGGCCTGAAGGCCTGGGCCGAGAACAACCAGCTGGCCGACCTGGCCGCGCTGTGCAGCCAGTTC‑GCCAGCCAGTCGCAGAGCGGCATcgcccgc                                                                                                            >  3:25138/1‑149 (MQ=255)                                             cGAAGGCACGCCGGACCGCACCCTGCACGAACAGCTGGTAAAGCCGCTGCACGGCCTGAAGGCCTGGGCCGAGAACAACCAGCTGGCCGACCTGGCCGCGCTGTGCAGCCAGTTC‑GCCAGCCAGTCGCAGAGCGGCATCGCCCGCctgc                                                                                                        >  6:599/1‑149 (MQ=255)                                              gAAGGCACGCCGGACCGCACCCTGCACGAACAGCTGGTAAAGCCGCTGCACGGCCTGAAGGCCTGGGCCGAGAACAACCAGCCGGCCGACCTGGCCGCGCTGTGCAGCCAGTTC‑GCCAGCCAGTCGCAGAGCGGCATCGCCCGCctgct                                                                                                       <  5:269121/149‑1 (MQ=255)                                                aGGCACGCCGGACCGCACCCTGCACGAACAGCTGGTAAAGCCGCTGCACGGCCTGAAGGCCTGGGCCGAGAACAACCAGCTGGCCGACCTGGCCGCGCTGTGCAGCCAGTTC‑GCCAGCCAGTCGCAGAGCGGCATCGCCCGCCtgctgc                                                                                                     <  6:169929/149‑1 (MQ=255)                                                       ccGGACCGCACCCTGCACGAACAGCTGGTAAAGCCGCTGCACGGCCTGAAGGCCTGGGCCGAGAACAACCAGCTGGCCGACCTGGCCGCGCTGTGCAGCCAGTTC‑GCCAGCCAGTCGCAGAGCGGCATCGCCCGCCTGCTGCCAGGccc                                                                                              <  7:35080/149‑1 (MQ=255)                                                       ccGGACCGCACCCTGCACGAACAGCTGGTAAAGCCGCTGCACGGCCTGAAGGCCTGGGCCGAGAACAACCAGCTGGCCGACCTGGCCGCGCTGTGCAGCCAGTTC‑GCCAGCCAGTCGCAGAGCGGCATCGCCCGCCTGCTGCCAGGc                                                                                                <  1:142959/147‑1 (MQ=255)                                                        cGGACCGCACCCTGCACGAACAGCTGGTAAAGCCGCTGCACGGCCTGAAGGCCTGGGCCGAGAACAACCAGCTGGCCGACCTGGCCGCGCTGTGCAGCCAg                                                                                                                                              >  2:50462/1‑101 (MQ=255)                                                        cGGACCGCACCCTGCACGAACAGCTGGTAAAGCCGCTGCACGGCCTGAAGGCCTGGGCCGAGAACAACCAGCTGGCCGACCTGGCCGCGCTGTGCAGCCAg                                                                                                                                              <  1:50462/101‑1 (MQ=255)                                                        cGGACCGCACCCTGCACGAACAGCTGGTAAAGCCGCTGCACGGCCTGAAGGCCTGGGCCGAGAACAACCAGCTGGCCGACCTGGCCGCGCTGTGCAGCCAGTTC‑GCCAGCCAGTCGCAGAGCGGCATCGCCCGCCTGCTGCCAGGccc                                                                                              >  5:156706/1‑148 (MQ=255)                                                           aCCGCACCCTGCACGAACAGCTGGTAAAGCCGCTGCACGGCCTGAAGGCCTGGGCCGAGAACAACCAGCTGGCCGACCTGGCCGCGCTGTGCAGCCAGTTC‑GCCAGCCAGTCGCAGAGCGGCATCGCCCGCCTGCTGCCAGGCCCGAcc                                                                                          >  5:91620/1‑149 (MQ=255)                                                              gCACCCTGCACGAACAGCTGGTAAAGCCGCTGCACGGCCTGAAGGCCTGGGCCGAGAACAACCAGCTGGCCGACCTGGCCGCGCTGTGCAGCCAGTTC‑GCCAGCCAGTCGCAGAGCGGCATCGCCCGCCTGCTGCCAGGCCCGACCGGc                                                                                       <  5:599/149‑1 (MQ=255)                                                                aCCCTGCACGAACAGCTGGTAAAGCCGCTGCACGGCCTGAAGGCCTGGGCCGAGAACAACCAGCTGGCCGACCTGGCCGCGCTGTGCAGCCAGTTC‑GCCAGCCAGTCGCAGAGCGGCATCGCCCGCCTGCTGCCAGGCCCGACCGGCGa                                                                                     <  4:72390/149‑1 (MQ=255)                                                                  ccTGCACGAACAGCTGGTAAAGCCGCTGCACGGCCTGAAGGCCTGGGCCGAGAACAACCAGCTGGCCGACCTGGCCGCGCTGTGCAGCCAGTTC‑GCCAGCCAGTCGCAGAGCGGCATCGCCCGCCTGCTGCCAGGCCCGACCGGCGGgc                                                                                   >  8:90049/1‑149 (MQ=255)                                                                  ccTGCACGAACAGCTGGTAAAGCCGCTGCACGGCCTGAAGGCCTGGGCCGAGAACAACCAGCTGGCCGACCTGGCCGCGCTGTGCAGCCAGTTC‑GCCAGCCAGTCGCAGAGCGGCATCGCCCGCCTGCTGCCAGGCCCGACCGGCGAgc                                                                                   >  6:94963/1‑149 (MQ=255)                                                                   cTGCACGAACAGCTGGTAAAGCCGCTGCACGGCCTGAAGGCCTGGGCCGAGAACAACCAGCTGGCCGACCTGGCCGCGCTGTGCAGCCAGTTC‑GCCAGCCAGTCGCAGAGCGGCATCGCCCGCCTGCTGCCAGGCCCGACCgg                                                                                        >  3:41053/1‑143 (MQ=255)                                                                   cTGCACGAACAGCTGGTAAAGCCGCTGCACGGCCTGAAGGCCTGGGCCGAGAACAACCAGCTGGCCGACCTGGCCGCGCTGTGCAGCCAGTTC‑GCCAGCCAGTCGCAGAGCGGCATCGCCCGCCTGCTGCCAGGCCCGACCgg                                                                                        <  4:41053/143‑1 (MQ=255)                                                                        cGAACAGCTGGTAAAGCCGCTGCACGGCCTGAAGGCCTGGGCCGAGAACAACCAGCTGGCCGACCTGGCCGCGCTGTGCAGCCAGTTC‑GCCAGCCAGTCGCAGAGCGGCATCGCCCGCCTGCTGCCAGGCCCGACCGGCGAgc                                                                                   >  8:276190/1‑143 (MQ=255)                                                                        cGAACAGCTGGTAAAGCCGCTGCACGGCCTGAAGGCCTGGGCCGAGAACAACCAGCTGGCCGACCTGGCCGCGCTGTGCAGCCAGTTC‑GCCAGCCAGTCGCAGAGCGGCATCGCCCGCCTGCTGCCAGGCCCGACCGGCGAgc                                                                                   <  7:276190/143‑1 (MQ=255)                                                                          aaCAGCTGGTAAAGCCGCTGCACGGCCTGAAGGCCTGGGCCGAGAACAACCAGCTGGCCGACCTGGCCGCGCTGTGCAGCCAGTTC‑GCCAGCCAGTCGCAGAGCGGCATCGCCCGCCTGCTGCCAGGCCCGACCGGCGAGCGCAACAGc                                                                           <  6:156706/149‑1 (MQ=255)                                                                            cAGCTGGTAAAGCCGCTGCACGGCCTGAAGGCCTGGGCCGAGAACAACCAGCTGGCCGACCTGGCCGCGCTGTGCAGCCAGTTC‑GCCAGCCAGTCGCAGAGCGGCATCGCCCGCCTGCTGCCAGGCCCGACCGGCGAGCGCAACAGCTa                                                                         <  1:11086/149‑1 (MQ=255)                                                                               cTGGTAAAGCCGCTGCACGGCCTGAAGGCCTGGGCCGAGAACAACCAGCTGGCCGACCTGGCCGCGCTGTGCAGCCAGTTC‑GCCAGCCAGTCGCAGAGCGGCATCGCCCGCCTGCTGCCAGGCCCGACCGGCGAGCGCAACAGCTacac                                                                      <  5:94963/149‑1 (MQ=255)                                                                                         cGCTGCACGACCTGAAGGCCTGGGCCGAGAACAACCAGCTGGCCGACCTGGCCGCGCTGTGCAGCCAGTTC‑GCCAGCCAGTCGCAGAGCGGCATCGCCCGCCTGCTGCCAGGCCCGACCGGCGAGCGCAACAGCTACACCATTCTGCcg                                                            >  5:59093/1‑149 (MQ=255)                                                                                                 ggCCTGAAGGCCTGGGCCGAGAACAACCAGCTGGCCGACCTGGCCGCGCTGTGCAGCCAGTTC‑GCCAGCCAGTCGCa                                                                                                                            <  2:314089/77‑1 (MQ=255)                                                                                                 ggCCTGAAGGCCTGGGCCGAGAACAACCAGCTGGCCGACCTGGCCGCGCTGTGCAGCCAGTTC‑GCCAGCCAGTCGCa                                                                                                                            >  1:314089/1‑77 (MQ=255)                                                                                                       aaGGCCTGGGCCGAGAACAACCAGCTGGCCGACCTGGCCGCGCTGTGCAGCCAGTTC‑GCCAGCCAGTCGCAGAGCGGCATCGCCCGCCTGCTGCCAGGCCCGACCGGCGAGCGCAACAGCTACACCATTCTGCCGCGTGAGCAc                                                   >  7:219115/1‑144 (MQ=255)                                                                                                       aaGGCCTGGGCCGAGAACAACCAGCTGGCCGACCTGGCCGCGCTGTGCAGCCAGTTC‑GCCAGCCAGTCGCAGAGCGGCATCGCCCGCCTGCTGCCAGGCCCGACCGGCGAGCGCAACAGCTACACCATTCTGCCGCGTGAGCAc                                                   <  8:219115/144‑1 (MQ=255)                                                                                                       aaGGCCTGGGCCGAGAACAACCAGCTGGCCGACCTGGCCGCGCTGTGCAGCCAGTTC‑GCCAGCCAGTCGCAGAGCGGCATCGCCCGCCTGCTGCCAGGCCCGACCGGCGAGCACAACAGCTACACCATTCTGCCGCGTGAGCACGTg                                                <  2:70183/147‑1 (MQ=255)                                                                                                          gCCTGGGCCGAGAACAACCAGCTGGCCGACCTGGCCGCGCTGTGCAGCCAGTTC‑GCCAGCCAGTCGCAGAGCGGCATCGCCCGCCTGCTGCCAGGCCCGACCGGCGAGCGCAACAGCTACACCATTCTGCCGCGTGAGCACGTGCtgtg                                           >  1:333926/1‑149 (MQ=255)                                                                                                             tGGGCCGAGAACAACCAGCTGGCCGACCTGGCCGCGCTGTGCAGCCAGTTC‑GCCAGCCAGTCGCAGAGCGGCATCGCCCGCCTGCTGCCAGGCCCGACCGGCGAGCGCAACAGCTACACCATTCTGCCGCGt                                                         <  6:136279/132‑1 (MQ=255)                                                                                                             tGGGCCGAGAACAACCAGCTGGCCGACCTGGCCGCGCTGTGCAGCCAGTTC‑GCCAGCCAGTCGCAGAGCGGCATCGCCCGCCTGCTGCCAGGCCCGACCGGCGAGCGCAACAGCTACACCATTCTGCCGCGt                                                         >  5:136279/1‑132 (MQ=255)                                                                                                               ggCCGAGAACAACCAGCTGGCCGACCTGGCCGCGCTGTGCAGCCAGTTC‑GCCAGCCAGTCGCAGAGCGGCATCGCCCGCCTGCTGCCAGGCCCGACCGGCGAGCGCAACAGCTACACCATTCTGCCGCGTGAGCACGTGCTGTGCCTg                                       <  6:59093/148‑1 (MQ=255)                                                                                                                gCCGAGAACAACCAGCTGGCCGACCTGGCCGCGCTGTGCAGCCAGTTC‑GCCAGCCAGTCGCAGAGCGGCATCGCCCGCCTGCTGCCAGGCCCGACCGGCGAGCGCAACAGCTACACCATTCTGCCGCGTGAGCACGTGCTGTGCCTGGc                                     <  4:25138/149‑1 (MQ=255)                                                                                                                       acaacCAGCTGGCCGACCTGGCCGCGCTGTGCAGCCAGTTC‑GCCAGCCAGTCGCAGAGCGGCATCGCCCGCCTGCTGCCAGGCCCGACCGGCGAGCGCAACAGCTACACCATTCTGCCGCGTGAGCACGTGCTGTGCCTGGCCGACAAc                              <  6:91620/149‑1 (MQ=255)                                                                                                                        caacCAGCTGGCCGACCTGGCCGCGCTGTGCAGCCAGTTC‑GCCAGCCAGTCGCAGAGCGGCATCGCCCGCCTGCTGCCAGGCCCGACCGGCGAGCGCAACAGCTACACCATTCTGCCGCGTGAGCACGTGCTGTGCCTGGCCGACAAc                              >  7:240746/1‑148 (MQ=255)                                                                                                                         aacCAGCTGGCCGACCTGGCCGCGCTGTGCAGCCAGTTC‑GCCAGCCAGTCGCAGAGCGGCATCGCCCGCCTGCTGCCAGGCCCGACCGGCGAGCGCAACAGCTACACCATTCTGCCGCGTGAGCACGTGCTGTGCCTGGc                                     <  5:184756/140‑1 (MQ=255)                                                                                                                         aacCAGCTGGCCGACCTGGCCGCGCTGTGCAGCCAGTTC‑GCCAGCCAGTCGCAGAGCGGCATCGCCCGCCTGCTGCCAGGCCCGACCGGCGAGCGCAACAGCTACACCATTCTGCCGCGTGAGCACGTGCTGTGCCTGGc                                     >  6:184756/1‑140 (MQ=255)                                                                                                                                tGGCCGACCTGGCCGCGCTGTGCAGCCAGTTC‑GCCAGCCAGTCGCAGAGCGGCATCGCCCGCCTGCTGCCAGGCCCGACCGGCGAGCGCAACAGCTACACCATTCTGCCGCGTGAGCACGTGCTGTGCCTGGCCGACAACGAAACCGa                      >  3:198841/1‑148 (MQ=255)                                                                                                                                  gCCGACCTGGCCGCGCTGTGCAGCCAGTTC‑GCCAGCCAGTCGCAGAGCGGCATCGCCCGCCTGCTGCCAGGCCCGACCGGCGAGCGCAACAGCTACACCATTCTGCCGCGTGAGCACGTGCTGTGCCTGGCCGACAACGAAACCGAc                     <  4:217281/147‑1 (MQ=255)                                                                                                                                  gCCGACCTGGCCGCGCTGTGCAGCCAGTTC‑GCCAGCCAGTCGCAGAGCGGCATCGCCCGCCTGCTGCCAGGCCCGACCGGCGAGCGCAACAGCTACACCATTCTGCCGCGTGAGCACGTGCTGTGCCTGGCCGACAACGAAACCGACct                   >  8:216409/1‑149 (MQ=255)                                                                                                                                   gcGAACTGGCTGCACAGCGCGGCCAGGTCGGCCAGCTAGTCGCAGAGCGGCATCGCCCGCCTGCTGCCAGGCCCGACCGGCGAGCGCAACAGCTACACCATTCTGCCGCGTGAGCACGTGCTGTGCCTGGCCGACAACGAAACCGAc                     <  7:201295/146‑1 (MQ=38)                                                                                                                                     gaacTGGCTGCACAGCGCGGCCAGTTC‑GCCAGCCAGTCGCAGAGCGGCATCGCCCGCCTGCTGCCAGGCCCGACCGGCGAGCGCAACAGCTACACCATTCTGCCGCGTGAGCACGTGCTGTGCCTGGCCGACAACGAAACCGACctgct                >  5:99084/4‑149 (MQ=255)                                                                                                                                      aCCTGGCCGCGCTGTGCAGCCAGTTC‑GCCAGCCAGTCGCAGAGCGGCATCGCCCGCCTGCTGCCAGGCCCGACCGGCGAGCGCAACAGCTACACCATTCTGCCGCGTGAGCACGTGCTGTGCCTGGCCGACAACGAAACCGACctgctg               >  5:253077/1‑149 (MQ=255)                                                                                                                                        cTGGCCGCGCTGTGCAGCCAGTTC‑GCCAGCCAGTCGCAGAGCGGCATCGCCCGCCTGCTGCCAGGCCCGACCGGCGAGCGCAACAGCTACACCATTCTGCCGCGTGAGCACGTGCTGTGCCTGGCCGACAACGAAACCGACCTGCTGgc             >  1:350341/1‑149 (MQ=255)                                                                                                                                           gCCGCGCTGTGCAGCCAGTTC‑GCCAGCCAGTCGCAGAGCGGCATCGCCCGCCTGCTGCCAGGCCCGACCGGCGAGCGCAACAGCTACACCATTCTGCCGCGTGAGCACGTGCTGTGCCTGGCCGACAACGAAACCGAcc                    >  3:65816/1‑139 (MQ=255)                                                                                                                                           gCCGCGCTGTGCAGCCAGTTC‑GCCAGCCAGTCGCAGAGCGGCATCGCCCGCCTGCTGCCAGGCCCGACCGGCGAGCGCAACAGCTACACCATTCTGCCGCGTGAGCACGTGCTGTGCCTGGCCGACAACGAAACCGAcc                    <  4:65816/139‑1 (MQ=255)                                                                                                                                           gCCGCGCTGTGCAGCCAGTTC‑GCCAGCCAGTCGCAGAGCGGCATCGCCCGCCTGCTGCCAGGCCCGACCGGCGAGCGCAACAGCTACACCATTCTGCCGCGTGAGCACGTGCTGTGCCTGGCCGACAACGAAACCGACCTGCTGgcg            >  2:246821/1‑147 (MQ=255)                                                                                                                                           gCCGCGCTGTGCAGCCAGTTC‑GCCAGCCAGTCGCAGAGCGGCATCGCCCGCCTGCTGCCAGGCCCGACCGGCGAGCGCAACAGCTACACCATTCTGCCGCGTGAGCACGTGCTGTGCCTGGCCGACAACGAAACCGACCTGCTGgcg            >  3:172187/1‑147 (MQ=255)                                                                                                                                            ccGCGCTGTGCAGCCAGTTC‑GCCAGCCAGTCGCAGAGAGGCATCGCCCTCCTGCTGCCAGGCCCGACCGGCGATCGCAACAGCTACACCATTCTGCCGCGTGAGCACGTGCTGTGCCTGGCCGACAACGAAACCGACCTGCTGGCGCAg         <  4:216379/149‑1 (MQ=255)                                                                                                                                             cgcgCTGTGCAGCCAGTTC‑GCCAGCCAGTCGCAGAGCGGCATCGCCCGCCTGCTGCCAGGCCCGACCGGCGAGCGCAACAGCTACACCATTCTGCCGCGTGAGCACGTGCTGTGCCTGGCCGACAACGAAACCGACCTGCTGGAGCAGt        >  6:252194/1‑149 (MQ=255)                                                                                                                                                    tgCAGCCAGTTC‑GCCAGCCAGTCGCAGAGCGGCATCGCCCGCCTGCTGCCAGGCCCGACCGGCGAGCGCAACAGCTACACCATTCTGCCGCGTGAGCACGTGCTGTGCCTGGCCGACAACGAAACCGACCTGCTGGCGCAGTTcgccg  <  1:246821/148‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                     CGCCCGGCCGACGCCATTGGCCGCCACTTCCAGCAGCAGGACGGCGAAGGCACGCCGGACCGCACCCTGCACGAACAGCTGGTAAAGCCGCTGCACGGCCTGAAGGCCTGGGCCGAGAACAACCAGCTGGCCGACCTGGCCGCGCTGTGCAGCCAGTTC‑GCCAGCCAGTCGCAGAGCGGCATCGCCCGCCTGCTGCCAGGCCCGACCGGCGAGCGCAACAGCTACACCATTCTGCCGCGTGAGCACGTGCTGTGCCTGGCCGACAACGAAACCGACCTGCTGGCGCAGTTCGCCG  >  NC_002947/5633792‑5634086

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑