| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,669,964 | C→G | 6.8% | H147Q (CAC→CAG) | metF → | 5,10‑methylenetetrahydrofolate reductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,669,964 | 0 | C | G | 6.8% | 232.4 / 5.6 | 73 | H147Q (CAC→CAG) | metF | 5,10‑methylenetetrahydrofolate reductase |
| Reads supporting (aligned to +/- strand): ref base C (36/32); new base G (3/2); total (39/34) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.74e-01 | |||||||||||
TGCTGGCCGAGTACAAGGCAGCCGGCATCAAACGCATTGTCGCGCTGCGTGGTGACCTGCCGTCGGGCATGGGCATGGCCAGTGGTGAACTGCGCTATGCCAGTGACC‑‑‑TGGTCGAGTTCATCCGCGAGGAGACCGCTGATCACTTCCAC‑CTGGAAGTGGCTGCCTACCCGGAAATGCACCCGCAGGCGCGCAACTTCGAGGCGGATCTGGCCAACTTTGTGCACAAGGTCAAGGCCGGTGCCGACAGCGCCATCACCCAGTACTTCTTCAACGCCGACAGCTACTTCTACTTCGTC > NC_002947/5669816‑5670111 | tgctgGCCGAGTACAAGGCAGCCGGCATCAAACGCATTGTCGCGCTGCGTGGTGACCTGCCGTCGGGCATGGGCATGGCCAGTGGTGAACTGCGCTATGCCAGTGACC‑‑‑TGGTCGAGTTCATCCGCGAGGAGACCGCTGATCACTTCCAc > 5:117583/1‑149 (MQ=255) gCCGAGTACAAGGCAGCCGGCATCAAACGCATTGTCGCGCTGCGTGGTGACCTGCCGTCGGGCATGGGCATGGCCAGTGGTGAACTGCGCTATGCCAGTGACC‑‑‑TGGTCGAGTTCATCCGCGAGGAGACCGCTGATCACTTCCAC‑CTgg < 8:126069/148‑1 (MQ=255) cGAGTACAAGGCAGCCGGCATCAAACGCATTGTCGCGCTGCGTGGTGACCTGCCGTCGGGCATGGGCATGGCCAGTGGTGAACTGCGCTATGCCAGTGACC‑‑‑TGGTCGAGTTCATCCGCGAGGAGACCGCTGATCACTTCCAC‑CTGGAAg > 1:105136/1‑149 (MQ=255) aCAAGGCAGCCGGCATCAAACGCATTGTCGCGCTGCGTGGTGACCTGCCGTCGGGCATGGGCATGGCCAGTGGTGAACTGCGCTATGCCAGTGACC‑‑‑TGGTCGAGTTCATCCGCGAGGAGACCGCTGATCACTTCCAC‑CTGGAAGTCGCt > 1:163608/1‑149 (MQ=255) cAAGGCAGCCGGCATCAAACGCATTGTCGCGCTGCGTGGTGACCTGCCGTCGGGCATGGGCATGGCCAGTGGTGAACTGCGCTATGCCAGTGACC‑‑‑TGGTCGAGTTCATCCGCGAGGAGACCGCTGATCACTTCCAC‑CTGGAAGTGGCt < 1:62430/148‑1 (MQ=255) cAAGGCAGCCGGCATCAAACGCATTGTCGCGCTGCGTGGTGACCTGCCGTCGGGCATGGGCATGGCCAGTGGTGAACTGCGCTATGCCAGTGACC‑‑‑TGGTCGAGTTCATCCGCGAGGAGACCGCTGATCACTTCCAC‑CTGGAAGTGGCTg < 8:145726/149‑1 (MQ=255) acggCAGCCGGCATCAAACGCTTGGTCGCGCTGCGTGGTGACCTGCCGTCGGGCATGGGCATGGCCAGTGGTGAACTGCGCTATGCCAGTGACC‑‑‑TGGGCGAGTTCATCCGCGAGGAGACCGCTGATCACTTCCAG‑GTGGAAGTgatcag < 8:299341/147‑6 (MQ=255) aGCCGGCATCAAACGCATTGTCGCGCTGCGTGGTGACCTGCCGTCGGGCATGGGCATGGCCAGTGGTGAGCTGCGCTATGCCAGTGACC‑‑‑TGGTCGAGTTCATCCGCGAGGAGACCGCTGATCACTTCCAC‑CTGGAAGTGGCTGCCTAcc > 5:57909/1‑149 (MQ=255) aGCCGGCATCAAACGCATTGTCGCGCTGCGTGGTGACCTGCCGTCGGGCATGGGCATGGCCAGTGGTGAACTGCGCTATGCCAGTGACC‑‑‑TGGTCGAGTTCATCCGCGAGGAGACCGCTGATCACTTCCAC‑CTGGAAGTGGCTGCCTAcc > 1:36832/1‑149 (MQ=255) gCCGGCATCAAACGCATTGTCGCGCTGCGTGGTGACCTGCCGTCGGGCATGGGCATGGCCAGTGGTGAGCTGCGCTATGCCAGTGACC‑‑‑TGGTCGAGTTCATCCGCGAGGAGACCGCTGATCACTTCCAC‑CTGGAAGTGGCTGCCTAccc < 6:57909/149‑1 (MQ=255) aTCAAACGCATTGTCGCGCTGCGTGGTGACCTGCCGTCGGGCATGGGCATGGCCAGTGGTGAACTGCGCTATGCCAGTGACC‑‑‑TGGTCGAGTTCATCCGCGAGGAGACCGCTGATCACTTCCAC‑CTGGAAGTGGCTGCCTACCCGGAAAt < 4:143735/149‑1 (MQ=255) cAAACGCATTGTCGCGCTGCGTGGTGACCTGCCGTCGGGCATGGGCATGGCCAGTGGTGAACTGCGCTATGCCAGTGACC‑‑‑TGGTCGAGTTCATCCGCGAGGAGACCGCTGATCACTTCCAC‑CTGGAAGTGGCTGCCTACCCGGaaa > 7:177045/1‑146 (MQ=255) cAAACGCATTGTCGCGCTGCGTGGTGACCTGCCGTCGGGCATGGGCATGGCCAGTGGTGAACTGCGCTATGCCAGTGACC‑‑‑TGGTCGAGTTCATCCGCGAGGAGACCGCTGATCACTTCCAC‑CTGGAAGTGGCTGCCTACCCGGaaa < 8:177045/146‑1 (MQ=255) aaaCGCATTGTCGCGCTGCGTGGTGACCTGCCGTCGGGCATGGGCATGGCCAGTGGTGAACTGCGCTATGCCAGTGACC‑‑‑TGGTCGAGTTCATCCGCGAGGAGACCGCTGATCACTTCCAC‑CTGGAAGTGGCTGCCTACCCGGAAATGCa < 1:150941/149‑1 (MQ=255) aaaCGCATTGTCGCGCTGCGTGGTGACCTGCCGTCGGGCATGGGCATGGCCAGTGGTGAACTGCGCTATGCCAGTGACC‑‑‑TGGTCGAGTTCATCCGCGAGGAGACCGCTGATCACTTCCAC‑CTGGAAGTGGCTGCCTACCCGGAAATGCa > 2:284722/1‑149 (MQ=255) aaaCGCATTGTCGCGCTGCGTGGTGACCTGCCGTCGGGCATGGGCATGGCCAGTGGTGAACTGCGCTATGCCAGTGACC‑‑‑TGGTCGAGTTCATCCGCGAGGAGACCGCTGATCACTTCCAC‑CTGGAAGTGGCTGCCTACCCGGAAATGCa > 2:152670/1‑149 (MQ=255) aCGCATTGTCGCGCTGCGTGGTGACCTGCCGTCGGGCATGGGCATGGCCAGTGGTGAACTGCGCTATGCCAGTGACC‑‑‑TGGTCGAGTTCATCCGCGAGGAGACCGCTGATCACTTCCAC‑CTGGAAGTGGCTGCCTACCCGGAAATGCAcc > 3:107913/1‑149 (MQ=255) gcgcTGCGTGGTGACCTGCCGTCGGGCATGGGCATGGCCAGTGGTGAACTGCGCTATGCCAGTGACCTGATGGGCGAGTTCATCCGCGAGGAGCCCGCTGATCAATTCCAC‑CTGGAAGTGGCTGCCTACCCGGAAATGCACCCGCAGgc > 1:112312/1‑149 (MQ=255) cgcTGCGTGGTGACCTGCCGTCGGGCATGGGCATGGCCAGTGGTGAACTGCGCTATGCCAGTGACC‑‑‑TGGTCGAGTTCATCCGCGAGGAGACCGCTGATCACTTCCAC‑CTGGAAGTGGCTGCCTACCCGGAAATg > 2:232069/1‑134 (MQ=255) cgcTGCGTGGTGACCTGCCGTCGGGCATGGGCATGGCCAGTGGTGAACTGCGCTATGCCAGTGACC‑‑‑TGGTCGAGTTCATCCGCGAGGAGACCGCTGATCACTTCCAC‑CTGGAAGTGGCTGCCTACCCGGAAATg < 1:232069/134‑1 (MQ=255) cGTGGTGACCTGCCGTCGGGCATGGGCATGGCCAGTGGTGAACTGCGCTATGCCAGTGACC‑‑‑TGGTCGAGTTCATCCGCGAGGAGACCGCTGATCACTTCCAC‑CTGGAAGTGGCTGCCTACCCGGAAATGCACCCGCAGGCGCGCAACtt < 2:105136/149‑1 (MQ=255) tgACCTGCCGTCGGGCATGGGCATGGCCAGTGGTGAACTGCGCTATGCCAGTGACC‑‑‑TGGTCGAGTTCATCCGCGAGGAGACCGCTGATCACTTCCAC‑CTGGAAGTGGCTGCCTACCCGGAAATGCACCCGCAGGCGCGCAACTTCGAgg > 8:30831/1‑149 (MQ=255) aCCTGCCGTCGGGCATGGGCATGGCCAGTGGTGAACTGCGCTATGCCAGTGACC‑‑‑TGGTCGAGTTCATCCGCGAGGAGACCGCTGATCACTTCCAC‑CTGGAAGTGGCTGCCTACCCGGAAATGCACCCGCAGGCGCGCAACTTCGAGGc < 6:316852/148‑1 (MQ=255) aCCTGCCGTCGGGCATGGGCATGGCCAGTGGTGAACTGCGCTATGCCAGTGACC‑‑‑TGGTCGAGTTCATCCGCGAGGAGACCGCTGATCACTTCCAC‑CTGGAAGTGGCTGCCTACCCGGAAATGCACCCGCAGGCGCGCAACTTCGAGGCg > 2:22563/1‑149 (MQ=255) cTGCCGTCGGGCATGGGCATGGCCAGTGGTGAACTGCGCTATGCCAGTGACC‑‑‑TGGTCGAGTTCATCCGCGAGGAGACCGCTGATCACTTCCAC‑CTGGAAGTGGCTGCCTACCCGGAAATGCACCCGCAGGCGCGCAACTTCGAGGCGGa > 4:189387/1‑149 (MQ=255) cTGCCGTCGGGCATGGGCATGGCCAGTGGTGAACTGCGCTATGCCAGTGACC‑‑‑TGGTCGAGTTCATCCGCGAGGAGACCGCTGATCACTTCCAC‑CTGGAAGTGGCTGCCTACCCGGAAATGCACACGCAGGCGCTCAACTTCGAGGCCGa > 6:270650/1‑149 (MQ=255) gCCGTCGGGCATGGGCATGGCCAGTGGTGAACTGCGCTATGCCAGTGACC‑‑‑TGGTCGAGTTCATCCGCGAGGAGACCGCTGATCACTTCCAC‑CTGGAAGTGGCTGCCTACCCGGAAATGCACCCGCAGGCGCGCAACTTCGAGGCGGATc > 4:294571/1‑149 (MQ=255) cGTCGGGCATGGGCATGGGCAGTGGTTAACTGCGCTATGCCAGTGAGC‑‑‑TGGTCGAGTTCATCCGCGAGGAGACCGCTGATCACTTCCAC‑CTGGAAGTGGCTGCCTACCCGGAAATGCACCCGCAGGCGCGCAACTTCGAGGCGGATCTg < 2:163608/149‑1 (MQ=255) tCGGGCATGGGCATGGCCAGTGGTGAACTGCGCTATGCCAGTGACC‑‑‑TGGTCGAGTTCATCCGCGAGGAGACCGCTGATCACTTCCAC‑CTGGAAGTGGCTGCCTACCCGGAAATGCACCCGCAGGCGCGCAACTTCGAGGCGGATCTGGc < 1:152670/149‑1 (MQ=255) ggggctgggcatgggcaGTGGTTAACTGCGCTATGCCATTGACC‑‑‑TGGGCGTTTTAAACCGCGATGTGAACGGTGCTCCCATTCACTTTGGAAGTGGCTGCCTACCCGGAAATGCACCCGCAGGCGCGCAACTTCGAGGCGTATCTGGc < 1:284722/143‑1 (MQ=255) gcatgggcatggCCAGTGGTGAACTGCGCTATGCCAGTGACC‑‑‑TGGTCGAGTTCATCCGCGAGGAGACCGCTGATCACTTCCAC‑CTGGAAGTGGCTGCCTACCCGGAAATGCACCCGCAGGCGCGCAACTTCGAGGCGGATCTGGCCAAc > 6:350479/1‑149 (MQ=255) atgggcatggCCAGTGGTGAACTGCGCTATGCCAGTGACC‑‑‑TGGTCGAGTTCATCCGCGAGGAGACCGCTGATCACTTCCAC‑CTGGAAGTGGCTGCCTACCCGGAAATGCACCCGCAGGCGCGCAACTTCGAGGCGGATCTGGCCAACtt > 2:231540/1‑149 (MQ=255) tgggcatggCCAGTGGTGAACTGCGCTATGCCAGTGACC‑‑‑TGGTCGAGTTCATCCGCGAGGAGACCGCTGATCACTTCCAC‑CTGGAAGTGGCTGCCTACCCGGAAATGCACCCGCAGGCGCGCAACTTCGAGGCGGATCTGGCCAACttt < 5:350479/149‑1 (MQ=255) gggcatggCCAGTGGTGAACTGCGCTATGCCAGTGACC‑‑‑TGGTCGAGTTCATCCGCGAGGAGACCGCTGATCACTTCCAC‑CTGGAAGTGGCTGCCTACCCGGAAATGCACCCGCAGGCGCGCAACTTCGAGGCGGATCTGGCCAACTTtg > 4:113876/1‑149 (MQ=255) ggcatggCCAGTGGTGAACTGCGCTATGCCAGTGACC‑‑‑TGGTCGAGTTCATCCGCGAGGAGACCGCTGATCACTTCCAC‑CTGGAAGTGGCTGCCTACCCGGAAATGCACCCGCAGGCGCGCAACTTCGAGGCGGATCTGGCCAACTTtgt < 2:112312/149‑1 (MQ=255) ggcatggCCAGTGGTGAACTGCGCTATGCCAGTGACC‑‑‑TGGTCGAGTTCATCCGCGAGGAGACCGCTGATCACTTCCAC‑CTGGAAGTGGCTGCCTACCCGGAAATGCACCCGCAGGCGCGCAACTTCGAGGCGGATCTGGCCAACTTtg < 3:189387/148‑1 (MQ=255) gcatggCCAGTGGTGAACTGCGCTATGCCAGTGACC‑‑‑TGGTCGAGTTCATCCGCGAGGAGACCGCTGATCACTTCCAC‑CTGGAAGTGGCTGCCTACCCGGAAATGCACCCGCGGGCGCGCAACTTCGAGGCGGATCTGGCCAACTTtgtg < 7:117831/149‑1 (MQ=255) gcatggCCAGTGGTGAACTGCGCTATGCCAGTGACC‑‑‑TGGTCGAGTTCATCCGCGAGGAGACCGCTGATCACTTCCAC‑CTGGAAGTGGCTGCCTACCCGGAAATGCACCCGCAGGCGCGCAACTTCGAGGCGGATCTGGCCAACttt > 4:218268/1‑146 (MQ=255) gcatggCCAGTGGTGAACTGCGCTATGCCAGTGACC‑‑‑TGGTCGAGTTCATCCGCGAGGAGACCGCTGATCACTTCCAC‑CTGGAAGTGGCTGCCTACCCGGAAATGCACCCGCAGGCGCGCAACTTCGAGGCGGATCTGGCCAACttt < 3:218268/146‑1 (MQ=255) ggCCAGTGGTGAACTGCGCTATGCCAGTGACC‑‑‑TGGTCGAGTTCATCCGCGAGGAGACCGCTGATCACTTCCAC‑CTGGAAGTGGCTGCCTACCCGGAAATGCACCCGCAGGCGCGCAACTTCGAGGCGGATCTGGCCAACTTTGTGcac > 7:82740/1‑148 (MQ=255) gCCAGTGGTGAACTGCGCTATGCCAGTGACC‑‑‑TGGTCGAGTTCATCCGCGAGGAGACCGCTGATCACTTCCAC‑CTGGAAGTGGCTGCCTACCCGGAAATGCACCCGCAGGCGCGCAACTTCGAGGCGGATCTGGCCAACTTTGTGCACaa < 7:30831/149‑1 (MQ=255) gtgAACTGCGCTATGGCAGTGACC‑‑‑TGGTCGAGTTCATCCGCGAGGAGACCGCTGATCACTTCCAC‑CTGGAAGTGGCTGGCTACCCTGAAATGCACCCGCAGGCGCGCAACTTCGAGGCGGATCTGGCCAACTTTGTGCACAAGGTCaa < 5:270650/148‑1 (MQ=255) aCTGCGCTATGCCAGTGACC‑‑‑TGGTCGAGTTCATCCGCGAGGAGACCGCTGATCACTTCCAC‑CTGGAAGTGGCTGCCTACCCGGAAATGCACCCGCAGGCGCGCAACTTCGAGGCGGATCTGGCCAACTTTGTGCACAAGGTCAAGGCCg < 1:231540/149‑1 (MQ=255) cTGCGCTATGCCAGTGACC‑‑‑TGGTCGAGTTCATCCGCGAGGAGACCGCTGATCACTTCCAC‑CTGGAAGTGGCTGCCTACCCGGAAATGCACCCGCAGGCGCGCAACTTCGAGGCGGATCTGGCCAACTTTGTGCACAAGGTCAAGGCCgg < 2:36832/149‑1 (MQ=255) cgcTATGCCAGTGACC‑‑‑TGGTCGAGTTCATCCGCGAGGAGACCGCTGATCACTTCCAC‑CTGGAAGTGGCTGCCTACCCGGAAATGCACCCGCAGGCGCGCAACTTCGAGGCGGATCTGGCCAACTTTGTGCACAAGGTCAAGGCCGGTGc < 3:294571/149‑1 (MQ=255) ccAGTGACC‑‑‑TGGTCGAGTTCATCCGCGAGGAGACCGCTGATCACTTCCAC‑CTGGAAGTGGCTGCCTACCCGGAAATGCACCCGCAGGCGCGCAACTTCGAGGCGGATCTGGCCAACTTTGTGCACAAGGTCAAGGCCGGTGCCGACAgc > 6:200928/1‑149 (MQ=255) c‑‑‑TGGTCGAGTTCATCCGCGAGGCGACCGCTGATCACTTCCAC‑CTGGAAGTGGCTGCCTCACGGGAAATGCACCCGCAGGCACGCGACTTGGAGGCGGATCTGGGCAACTTTGTGCGCAAGGTCAGGGCCGGTGCGGCCAGCGCCATctc > 7:235182/1‑147 (MQ=255) c‑‑‑TGGTCGAGTTCATCCGCGAGGAGACCGCTGATCACTTCCAC‑CTGGAAGTGGCTGCCTACCCGGAAATGCACCCGCAGGCGCGCAACTTCGAGGCGGATCTGGCCAACTTTGTGCACAAGGTCAAGGCCGGTGCCGACAgcgc < 4:339510/143‑1 (MQ=255) c‑‑‑TGGTCGAGTTCATCCGCGAGGAGACCGCTGATCACTTCCAC‑CTGGAAGTGGCTGCCTACCCGGAAATGCACCCGCAGGCGCGCAACTTCGAGGCGGATCTGGCCAACTTTGTGCACAAGGTCAAGGCCGGTGCCGACAgcgc > 3:339510/1‑143 (MQ=255) c‑‑‑TGGTCGAGTTCATCCGCGAGGAGACCGCTGATCACTTCCAC‑CTGGAAGTGGCTGCCTACCCGGAAATGCACCCGCAGGCGCGCAACTTCGAGGCGGATCTGGCCAACTTTGTGCACAAGGTCAAGGCCGGTGCCGACAGCGCCATCAc > 7:235178/1‑149 (MQ=255) tGGTCGAGTTCATCCGCGAGGAGACCGCTGATCACTTCCAC‑CTGGAAGTGGCTGCCTACCCGGAAATGCACCCGCAGGCGCGCAACTTCGAGGCGGATCTGGCCAACTTTGTGCACAAGGTCAAGGCCGGt < 5:68450/131‑1 (MQ=255) tGGTCGAGTTCATCCGCGAGGAGACCGCTGATCACTTCCAC‑CTGGAAGTGGCTGCCTACCCGGAAATGCACCCGCAGGCGCGCAACTTCGAGGCGGATCTGGCCAACTTTGTGCACAAGGTCAAGGCCGGt > 6:68450/1‑131 (MQ=255) ggTCGAGTTCATCCGCGAGGAGACCGCTGATCACTTCCAC‑CTGGAAGTGGCTGCCTACCCGGAAATGCACCCGCAGGCGCGCAACTTCGAGGCGGATCTGGCCAACTTTGTGCACAAGGTCAAg < 3:248131/124‑1 (MQ=255) ggTCGAGTTCATCCGCGAGGAGACCGCTGATCACTTCCAC‑CTGGAAGTGGCTGCCTACCCGGAAATGCACCCGCAGGCGCGCAACTTCGAGGCGGATCTGGCCAACTTTGTGCACAAGGTCAAGGCCGGTGcc > 8:49644/1‑133 (MQ=255) ggTCGAGTTCATCCGCGAGGAGACCGCTGATCACTTCCAC‑CTGGAAGTGGCTGCCTACCCGGAAATGCACCCGCAGGCGCGCAACTTCGAGGCGGATCTGGCCAACTTTGTGCACAAGGTCAAGGCCGGTGcc < 7:49644/133‑1 (MQ=255) ggTCGAGTTCATCCGCGAGGAGACCGCTGATCACTTCCAC‑CTGGAAGTGGCTGCCTACCCGGAAATGCACCCGCAGGCGCCCAACATCGAGGCGGATCAGGCCAACTTTGTGCACAAAGTCAAg > 4:248131/1‑124 (MQ=255) gTCGAGTTCATCCGCGAGGAGACCGCTGATCACTTCCAC‑CTGGAAGTGGCTGCCTACCCGGAAATGCACCCGCAGGCGCGCAACTTCGAGGCGGATCTGGCCAACTTTGTGCACAAGGTCAAGGCCGGTGCCGACAGCGCCATCAcc < 8:82740/147‑1 (MQ=255) gTCGAGTTCATCCGCGAGGAGACCGCTGATCACTTCCAC‑CTGGAAGTGGCTGCCTACCCGGAAATGCACCCGCAGGCGCGCAACTTCGAGGCGGATCTGGCCAACTTTGTGCACAAGGTCAAGGCCGGTGCCGACAGCGCCATCAcc > 8:79505/1‑147 (MQ=255) gTCGAGTTCATCCGCGAGGAGACCGCTGATCACTTCCAC‑CTGGAAGTGGCTGCCTACCCGGAAATGCACCCGCAGGCGCGCAACTTCGAGGCGGATCTGGCCAACTTTGTGCACAAGGTCAAGGCCGGTGCCGACAGCGCCATCACCCa < 4:107913/149‑1 (MQ=255) gTCGAGTTCATCCGCGAGGAGACCGCTGATCACTTCCAC‑CTGGAAGTGACTGCCTACCCGGAAATGCACCCGCAGGCGCGCAACTTCGAGGCGGATCTGGCCAACTTTGTGCACAAGGTCAAGGCCGGTGCCGACAGCGCCATCACCCa < 6:117583/149‑1 (MQ=255) aTCCGCGAGGAGACCGCTGATCACTTCCAC‑CTGGAAGTGGCTGCCTACCCGGAAATGCACCCGCAGGCGCGCAACTTCGAGGCGGATCTGGCCAACTTTGTGCACAAGGTCAAGGCCGGTGCCGACAGCGCCATCACCCAGTActtct < 5:200928/148‑1 (MQ=255) tCCGCGAGGAGACCGCTGATCACTTCCAC‑CTGGAAGTGGCTGCCTACCCGGAAATGCACCCGCAGGCGCGCAACTTCGAGGCGGATCTGGCCAACTTTGTGCACAAGGTCAAGGCCGGTGCCGACAGCGCCATCACCCAGTActtctt > 4:83580/1‑148 (MQ=255) gcgAGGAGACCGCTGATCACTTCCAC‑CTGGAAGTGGCTGCCTACCCGGAAATGCACCCGCAGGCGCGCAACTTCGAGGCGGATCTGGCCAACTTTGTGCACAAGGTCAAGGCCGGTGCCGACAGCGCCATCACCCAGTACTTCTTCAAc > 7:224490/1‑149 (MQ=255) cGCTGATCACTTCCAC‑CTGGAAGTGGCTGCCTACCCGGAAATGCACCCGCAGGCGCGCAACTTCGAGGCGGATCTGGCCAACTTTGTGCACAAGGTCAAGGCCGGTGCCGACAGCGCCATCACCCAGTACTTCTTCAACGCCGACAGc > 2:236163/1‑148 (MQ=255) cGCTGATCACTTCCAC‑CTGGAAGTGGCTGCCTACCCGGAAATGCACCCGCAGGCGCGCAACTTCGAGGCGGATCTGGCCAACTTTGTGCACAAGGTCAAGGCCGGTGCCGACAGCGCCATCACCCAGTACTTCTTCAACGCCGACAGc > 1:220731/1‑148 (MQ=255) cGCTGATCACTTCCAC‑CTGGAAGTGGCTGCCTACCCGGAAATGCACCCGCAGGAGCGCAACTTCGAGGCGGATCTGGCCAACTTTGTGCACAAGGTCAAGGCCGGTGCCGACAGCGCCATCACCCAGTACTTCTTCAACGCCGACAGc > 5:334159/1‑148 (MQ=255) gCTGATCACTTCCAC‑CTGGAAGTGGCTGCCTACCCGGAAATGCACCCGCAGGCGCGCAACTTCGAGGCGGATCTGGCCAACTTTGTGCACAAGGTCAAGGCCGGTGCCGACAGCGACATAACCCAGTACTTCTTCAACGCCGACAGcta > 7:169020/1‑149 (MQ=255) cagccACTTCCAG‑GTGGAAGTGGCTGCCTACCCGGAAATGCACCCGCAGGCGCGCAACTTCGAGGCGGATCTGGCCAACTTTGTGCACAAGGTCAAGGCCGGTGCCGACAGCGCCATCACCCAGTACTTCTTCAACGCCGACAGctact > 7:200533/5‑149 (MQ=255) agccACTTCCAG‑GTGGAAGTGGCTGCCTACCCGGAAATGCACCCGCAGGCGCGCAACTTCGAGGCGGATCTGGCCAACTTTGTGCACAAGGTCAAGGCCGGTGCCGACAGCGCCATCACCCAGTACttcttc < 2:335324/129‑1 (MQ=255) agccACTTCCAG‑GTGGAAGTGGCTGCCTACCCGGAAATGCACCCGCAGGCGCGCAACTTCGAGGCGGATCTGGCCAACTTTGTGCACAAGGTCAAGGCCGGTGCCGACAGCGCCATCACCCAGTACttcttc > 1:335324/4‑132 (MQ=255) ccACTTCCAG‑GTGGAAGTGGCTGCCTACCCGGAAATGCACCCGCAGGCGCGCAACTTCGAGGCGGATCTGGCCAACTTTGTGCACAAGGTCAAGGCCGGTGCCGACAGCGCCATCACCCAGTACTTCTTCAACGCCGACAGctacttc > 5:323659/2‑148 (MQ=255) cACTTCCAC‑CTGGAAGTGGCTGCCTACCCGGAAATGCACCCGCAGGCGCGCAACTTCGAGGCGGATCTGGCCAACTTTGTGCACAAGGTCAAGGCCGGTGCCGACAGCGCCATCACCCAGTACTTCTTCAACGCCGACAGctacttct > 4:341094/1‑148 (MQ=255) ttCCAC‑CTGGAAGTGGCTTCCTACACGGAAATGCACCCGAAGGCGCGCAACTTCGAGGCGGATCTGGCCAACTTTGTGCACAAGGTCAAGGCCGGTGCCGACAGCGCCATCACCAAGTACTTCTTCAACGACGAAAGctacttctact > 5:259851/1‑148 (MQ=255) aC‑CTGGAAGTGGCTGCCTACCCGGAAATGCACCCGCAGGCGCGCAACTTCGAGGCGGATCTGGCCAACTTTGTGCACAAGGTCAAGGCCGGTGCCGACAGCGCCATCACCCAGTACTTCTTCAACGCCGACAGCTACTTCTACTtcgtc < 3:113876/149‑1 (MQ=255) | TGCTGGCCGAGTACAAGGCAGCCGGCATCAAACGCATTGTCGCGCTGCGTGGTGACCTGCCGTCGGGCATGGGCATGGCCAGTGGTGAACTGCGCTATGCCAGTGACC‑‑‑TGGTCGAGTTCATCCGCGAGGAGACCGCTGATCACTTCCAC‑CTGGAAGTGGCTGCCTACCCGGAAATGCACCCGCAGGCGCGCAACTTCGAGGCGGATCTGGCCAACTTTGTGCACAAGGTCAAGGCCGGTGCCGACAGCGCCATCACCCAGTACTTCTTCAACGCCGACAGCTACTTCTACTTCGTC > NC_002947/5669816‑5670111 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |