Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,671,667 T→A 8.3% intergenic (+86/‑84) PP_4978 → / → PP_4979 hypothetical protein/substrate‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,671,6670TA 8.3% 126.0 / 3.1 48intergenic (+86/‑84)PP_4978/PP_4979hypothetical protein/substrate‑binding protein
Reads supporting (aligned to +/- strand):  ref base T (23/21);  new base A (2/2);  total (25/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.93e-01

TCGCTGGGCGAGCGGCGCAAGGATATCGAGGCGCATCTGCTGCGATCCACCCAAGCCTGATGTGCCCGCAAAACTCATTGTCTTGCGCGGTCTTGTGGGAGCGGGCGTGCCCGCGAAGAGAGCAACCCGATCGTTGGCACCGGCTTCGCCGGTGTTCGCGGGCACGCCCGCTCCCACATCGATAATGCATGCCTTTACCGTACACCGGCCGAGCGCGTAATCTCCGGCCATGCCACATATCGCCCGTCTGTTCTGGATCCTGCTGCTCGTTTGCCTGAGCCCGTTGGCTCTGG  >  NC_002947/5671522‑5671814
                                                                                                                                                 |                                                                                                                                                   
tCGCTGGGCGAGCGGCGCAAGGATATCGAGGCGCATCTGCTGCGATCCACCCAAGCCTGATGTGCCCGCAAAACTCATTGTCTTGCGCGGTCTTGTGGGAGCGGGCGTGCCCGCGAAGAGAGCAACCCGATCGTTGGCACCGGCTTCGc                                                                                                                                                  <  6:137441/149‑1 (MQ=255)
 cGCTGGGCGAGCGGCGCAAGGATATCGAGGCGCATCTGCTGCGATCCACCCAAGCCTGATGTGCCCGCAAAACTCATTGTCTTGCGCGGTCTTGTGGGAGCGGGCGTGCCCGCGAAGAGAGCAACCCGATCGTTGGCACCGGCTTCGcc                                                                                                                                                 >  6:138592/1‑149 (MQ=255)
  gCTGGGCGAGCGGCGCAAGGATATCGAGGCGCATCTGCTGCGATCCACCCAAGCCTGATGTGCCCGCAAAACTCATTGTCTTGCGCGGTCTTGTGGGAGCGGGCGTGCCCGCGAAGAGAGCAACCCGATCGTTGGCACCGGCTTCGCCg                                                                                                                                                <  8:26772/149‑1 (MQ=255)
  gCTGGGCGAGCGGCGCAAGGATATCGAGGCGCATCTGCTGCGATCCACCCAAGCCTGATGTGCCCGCAAAACTCATTGTCTTGCGCGGTCTTGTGGGAGCGGGCGTGCCCGCGAAGAGAGCAACCCGATCGTTGGCACCGGCTTCGCCg                                                                                                                                                >  7:26772/1‑149 (MQ=255)
      ggCGAGCGGCGCAAGGATATCGAGGCGCATCTGCTGCGATCCACCCAAGCCTGATGTGCCCGCAAAACTCATTGTCTTGCGCGGTCTTGTGGGAGCGGGCGTGCCCGCGAAGAGAGCAACCCGATCGTTGGCACCGGCGAAGCCGgtg                                                                                                                                             <  1:70906/148‑1 (MQ=255)
          aGCGGCGCAAGGATATCGAGGCTCATCTGCTGCGATCCACCCAAGCCTGATGTGCCCGCAAAACTCATTGTCTTGCGCGGTCTTGTGGGAGCGGGCGTGCCCGCGAAGAGAGCAACCCGATCGTTGGCACCGGCTTCGCCGGTGTTcgc                                                                                                                                        <  4:111593/149‑1 (MQ=255)
                gcAAGGATATCGAGGCGCATCTGCTGCGATCCACCCAAGCCTGATGTGCCCGCAAAACTCATTGTCTTGCGCGGTCTTGTGGGAGCGGGCGTGCCCGCGAAGAGAGCAACCCGATCGTTGGCACCGGCTTCGCCGGTGTTCGCgg                                                                                                                                      <  8:39698/145‑1 (MQ=255)
                gcAAGGATATCGAGGCGCATCTGCTGCGATCCACCCAAGCCTGATGTGCCCGCAAAACTCATTGTCTTGCGCGGTCTTGTGGGAGCGGGCGTGCCCGCGAAGAGAGCAACCCGATCGTTGGCACCGGCTTCGCCGGTGTTCGCgg                                                                                                                                      >  7:39698/1‑145 (MQ=255)
                      atatCGAGGCGCATCTGCTGCGATCCACCCAAGCCTGATGTGCCCGCAAAACTCATTGTCTTGCGCGGTCTTGTGGGAGCGGGCGTGCCCGCGAAGAGAGCAACCCGATCGTTGGCACCGGCTTCGCCGGTGTTCGCGGGCACGCCCGc                                                                                                                            >  7:101163/1‑149 (MQ=255)
                         tCGAGGCGCATCTGCTGCGATCCACCCAAGCCTGATGTGCCCGCAAAACTCATTGTCTTGCGCGGTCTTGTGGGAGCGGGCGTGCCCGCGAAGAGAGCAACCCGATCGTTGGCACCGGCTTCGCCGGTGTTCGCGGGCACGCCCGCTcc                                                                                                                         <  2:273495/149‑1 (MQ=255)
                                      gctgcGATCCACCCAAGCCTGATGTGCCCGCAAAACTCATTGTCTTGCGCGGTCTTGTGGGAGCGGGCGTGCCCGCGAAGAGAGCAACCCGATCGTTGGCACCGGCTTCGCCGGTGTTCGCGGGCACGCCCGCTCCCACATCGATAatg                                                                                                            <  5:138592/149‑1 (MQ=255)
                                           gATCCACCCAAGCCTCATGTGCCCGCAAAACTCATTGTCTTGCGCGGTCTTGTGGGAGCGGGCGTGCCCGCGAAGAGAGCAACCCGATCGTTGGCACGGGCTTCGCCGGTGTTGGCGGGCACGCCCGCTCCCACATCGATAatgcatgc                                                                                                       <  3:181944/149‑1 (MQ=255)
                                                aCCCAAGCCTGATGTGCCCGCAAAACTCATTGTCTTGCGCGGTCTTGTGGGAGCGGGCGTGCCCGCGAAGAGAGCAACCCGATCGTTGGCACCGGCTTCGCCGGTGTTCGCGGGc                                                                                                                                    >  3:347499/1‑115 (MQ=255)
                                                aCCCAAGCCTGATGTGCCCGCAAAACTCATTGTCTTGCGCGGTCTTGTGGGAGCGGGCGTGCCCGCGAAGAGAGCAACCCGATCGTTGGCACCGGCTTCGCCGGTGTTCGCGGGc                                                                                                                                    <  4:347499/115‑1 (MQ=255)
                                                           aTGTGCCCGCAAAACTCATTGTCTTGCGCGGTCTTGTGGGAGCGGGCGTGCCCGCGAAGAGAGCAACCCGATCGTTGGCACCGGCTTc                                                                                                                                                    >  2:315417/1‑88 (MQ=255)
                                                           aTGTGCCCGCAAAACTCATTGTCTTGCGCGGTCTTGTGGGAGCGGGCGTGCCCGCGAAGAGAGCAACCCGATCGTTGGCACCGGCTTc                                                                                                                                                    <  1:315417/88‑1 (MQ=255)
                                                           aTGTGCCCGCAAAACTCATTGTCTTGCGCGGTCTTGTGGGAGCGGGCGTGCCCGCGAAGAGAGCAACCCGATCGTTGGCACCGGCTTCGCCGGTGTTCGCGGGCACGCCCGCTCCCACATCGATAATGCATGCCTTTACCGTACACCg                                                                                        <  8:101163/148‑1 (MQ=255)
                                                                cccGCAAAACTCATTGTCTTGCGCGGTCTTGTGGGAGCGGGCGTGCCCGCGAAGAGAGCAACCCGATCGTTGGCACCGGCTTAGCCGGTGTTCGCGGGCACGCCCGCTCCCACAACCATAATGCATGCCTTTACCGTACACCGGCCgcg                                                                                  >  3:126759/1‑147 (MQ=255)
                                                                                   tGCGCGGTCTTGTGGGAGCGGGCGTGCCCGCGAAGAGAGCAACCCGATCGTTTGCACCGGCTTCGCCGGTGTTAGCGGGCACGCCCGCGCCCACATAGATAAAGCATGCCTTTACCGTACACCCGCCGAGCCCGTAATCTACGGCCaag                                                               >  8:174490/1‑147 (MQ=25)
                                                                                    gcgcGGTCTTGTGGTCGCGGGCGGGCCCGCGAAGAGAGCAACCCGATCGTTGCCACCGGCTTCGCCGGTGTTCGCGGGCACGCCCGCTCCCACATCGATAATGCATGCCTTTACCGTACACCGGCCGAGCGCGTAATCTCCGGCCAt                                                                <  6:368430/147‑1 (MQ=255)
                                                                                       cgGTCTTGTGGGAGTGGGGTGGTCCGCGATGAGATCAACCCGATCGTTGGCACCGTCTTCGCTGGTGTTCGCGGGCACGCCCGCTCCCACATCGATAATGCATGCCTTTACGGTACACCGTCCGAGCGCGTAATCTCCGGCCATGCcac                                                           <  1:114240/149‑1 (MQ=37)
                                                                                         gtgttGTGGGAGCGGGCGGCCCCGCTAAGACACAAACCCGATCGTGGGCACCGGCTTCGCCGGTGTTCGCGGCCACGCCCGCTCCCACATCGATAATGCATGCCTTTACCGTACACCGGCCGAGCGCGTAATCTCCGGCCATGCCACat                                                         <  5:52788/146‑1 (MQ=38)
                                                                                            ttGTGGGAGCGGGCGTGCCCGCGAAGAGAGCAACCCGATCGTTGGCACCGGCTTCGCCGGTGTTCGCGGGCACGCCCGCTCCCACATCGATAATGCATGCCTTTACCGTACACCGGCCGAGCGCGTAATCTCCGGCCATGCCACATATc                                                      >  3:175508/1‑149 (MQ=255)
                                                                                               tgGGAGCGGGCGTGCCCGCGAAGAGAGCAACCCGATCGTTGGCACCGGCTTCGCCGGTGTTCGCGGGCACGCCCGCTCCCACATCGATAatgcatgc                                                                                                       <  7:220163/97‑1 (MQ=255)
                                                                                               tgGGAGCGGGCGTGCCCGCGAAGAGAGCAACCCGATCGTTGGCACCGGCTTCGCCGGTGTTCGCGGGCACGCCCGCTCCCACATCGATAatgcatgc                                                                                                       >  8:220163/1‑97 (MQ=255)
                                                                                                gggAGCGGGCGTGCCCGCGAAGAGAGCAACCCGATCGTTGGCACCGGCTTCGCCGGTGTTCGCGGGCACGCCCGCTCCCACATCGCTAATGCATGCCTTTACCGTACACCGGCCGAGCGCGTAATCTCCGCCCATGCCACATATCGccc                                                  >  1:141837/1‑149 (MQ=255)
                                                                                                 ttcgCGGGCACGCCCGCGAAGAGAGCAACCCGATCGTTGGCACCGGCTTCGCCGGTGTTCGCGGGCACGCCCGCTCCCACATCGATAATGCATGCCTTTACCGTACACCGGCCGAGCGCGTAATCTCCGGCCATGCCACATATCGCCCg                                                 >  6:19155/4‑149 (MQ=255)
                                                                                                    gCGGGCGTGCCCGCGAAGAGAGCAACCCGATCGTTGGCACCGGCTTCGCCGGTGTTCGCGGGCACGCCCGCTCCCACATCCATAATGCATGCCATTACCGTACACCGGCCGACCGCGTAATCTCCGGCCATGCCACATATAGCCCGTCt                                              >  2:34943/1‑149 (MQ=255)
                                                                                                      gggCGTGCCCGCGAAGAGAGCAACCCGATCGTTGGCACCGGCTTCGCCGGTGTTCGCGGGCACGCCCGCTCCCACATCGATAATGCATGCCTTTACCGTACACCGGCCGAGCGCGTAATCTCCGGCCATGCCACATATCGCCCGTCTGt                                            >  4:345599/1‑149 (MQ=255)
                                                                                                        gCGTGCCCGCGAAGAGAGCAACCCGATCGTTGGCACCGGCTTCGCCGGTGTTCGCGGGCACGCCCGCTCCCACATCGATAATGCATGCCTTTACCGTACACCGGCCGAGCGCGTAATCTCCGGCCATGCCACATATCGCCCGTCTGTTc                                          >  5:101975/1‑149 (MQ=255)
                                                                                                        gCGTGCCCGCGAAGAGAGCAACCCGATCGTTGGCACCCGCTTCGCCGGTGTTAGCGGGAACGCCCGCTCCCACATCGATAATGCATGCCATTACCGTACACCGGCCGAGCGCGTAATAACCGGCCATGCCACAAATCGCCCGTCTCTTc                                          >  4:209817/1‑149 (MQ=255)
                                                                                                         cacgCCCGCGAAGAGAGCAACCCGATCGTTGGCACCGGCTTCGCCGGTGTTCGCGGGCACGCCCGCTCCCACATCGATAATGCATGCCTTTACCGTACACCGGCCGAGCGCGTAATCTCCGGCCATGCCACATATCGCCCGTCTGTTCt                                         <  5:19155/146‑1 (MQ=255)
                                                                                                         cGTGCCCGCGAAGAGAGCAACCCGATCGTTGGCACCGGCTTCGCCGGTGTTCGCGGGCACGCCCGCTCCCACATCGATAATGCATGCCTTTACCGTACACCGGCCGAGCGCGTAATCTCCGGCCATGCCACATATCGCCCGTCTGTTCt                                         <  4:175508/149‑1 (MQ=255)
                                                                                                               cgcgAAGAGAGCAACCCGATCGTTGGCACCGGCTTCGCCGGTGTTCGCGGGCACGCCCGCTCCCACATCGATAATGCATGCCTTTACCGTACACCGGCCGAGCGCGTAATCTCCGGCCATGCCACATATCGCCCGTCTGTTCTGGATcc                                   <  4:349380/149‑1 (MQ=255)
                                                                                                                 cgAAGAGAGCAACCCGATCGTTGGAACCGTCTTCGCCGGTGGTCGCGGGCACGCCCGCGCCAACATCGATAATGCATGCCTTTACCGTACACCGGCCGAGCGCGTAATCTCCGGCCATGCCACATATCGCCCGTCTGTTCTGGATCctg                                 <  4:52648/149‑1 (MQ=255)
                                                                                                                   aaGAGAGCAACCCGATCGTTGGCACCGGCTTCGCCGGTGTTCGCGGGCACGCCCGCTCCCACATCGATAATGCATGCCTTTACCGTACACCGGCCGAGCGCGTAATCTCCGGCCATGCCACATATCGCCCGTCTGTTCTGGATCctgct                               <  7:337580/149‑1 (MQ=255)
                                                                                                                          cAACCCGATCGTTGGCACCGGCTTCGCCGGTGTTCGCGGGCACGCCCGCTCCCACATCGATAATGCATGCCTTTACCGTACACCGGCCGAGCGCGTAATCTCCGGCCATGCCACATATCGCCCGTCTGTTCt                                         >  6:209291/1‑132 (MQ=255)
                                                                                                                          cAACCCGATCGTTGGCACCGGCTTCGCCGGTGTTCGCGGGCACGCCCGCTCCCACATCGATAATGCATGCCTTTACCGTACACCGGCCGAGCGCGTAATCTCCGGCCATGCCACATATCGCCCGTCTGTTCTGGATCCTGCTGCTCGtt                        >  1:146714/1‑149 (MQ=255)
                                                                                                                           aaCCCGATCGTTGGCACCGGCTTCGCCGGTGTTCGCGGGCACGCCCGCTCCCACATCGATAATGCATGCCTTTACCGTACACCGGCCGAGCGCGTAATCTCCGGCCATGCCACATATCGCCCGTCTGTTCTGGATCCTGCTGCTCGttt                       <  7:174490/149‑1 (MQ=255)
                                                                                                                             cccGATCGTTGGCACCGGCTTCGCCGGTGTTCGCGGGCACGCCCGCTCCCACATCGATAATGCATGCCTTTACCGTACACCGGCCGAGCGCGTAATCTCCGGCCATGCCACATATCGCCCGTCTGTTCTGGATCCTGCTGCTCGttt                       <  6:136313/147‑1 (MQ=255)
                                                                                                                             cccGATCGTTGGCACCGGCTTCGCCGGTGTTCGCGGGCACGCCCGCTCCCACATCGATAATGCATGCCTTTACCGTACACCGGCCGAGCGCGTAATCTCCGGCCATGCCACATATCGCCCGTCTGTTCTGGATCCTGCTGCTCGttt                       >  5:136313/1‑147 (MQ=255)
                                                                                                                             cccGATCGTTGGCACCGGCTTCGCCGGTGTTCGCGGGCACGCCCGCTCCCACATCGATAATGCATGCCTTTACCGTACACCGGCCGAGCGCGTAATCTCCGGCCATGCCACATATCGCCCGTCTGTTCTGGATCCTGCTGCTCGTTTGc                     >  4:221224/1‑149 (MQ=255)
                                                                                                                              ccGATCGTTGGCACCGGCTTCGCCGGTGTTCGCGGGCACGCCCGCTCCCACATCGATAATGCATGCCTTTACCGTACACCGGCCGAGCGCGTAATCTCCGGCCATGCCACATATCGCCCGTCTGTTCTGGATCCTGCTGCTCGTTTGcc                    >  6:3837/1‑149 (MQ=255)
                                                                                                                               cGATCGTTGGCACCGGCTTCGCCGGTGTTCGCGGGCACGCCCGCTCCCACATCGATAATGCATGCCTTTACCGTACACCGGCCGAGCGCGTAATCTCCGGCCATGCCACATATCGCCCGTCTGTTCTGGATCCTGCTGCTCGTTTGCCt                   >  1:141443/1‑149 (MQ=255)
                                                                                                                                gATCGTTGGCACCGGCTTCGCCGGTGTTCGCGGGCACGCCCGCTCCCACATCGATAATGCATGCCTTTACCGTACACCGGCCGAGCGCGTAATCTCCGGCCATGCCACATATCGCCCGTCTGTTCTGGATCCTGCTGCTCGTTTGCCt                   >  8:104926/1‑148 (MQ=255)
                                                                                                                                    gcgaacACCGGCGAAGCCGGTGTTCGCGGGCACGCCCGCTCCCACATCGATAATGCATGCCTTTACCGTACACCGGCCGAGCGCGTAATCTCCGGCCATGCCACATATCGCCCGTCTGTTCTGGATCCTGCTGCTCGTTTGCCTGAGcc              >  2:21647/6‑149 (MQ=255)
                                                                                                                                     cgaacACCGGCGAAGCCGGTGCTCGCGGGCACGCCCGCTCCCACATCGATAATGCATGCCTTTACCGTACACCGGCCGAGCGCGTAATCTCCGGCCATGCCACATATCGCCCGTCTGtt                                           <  6:341641/115‑1 (MQ=39)
                                                                                                                                     cgaacACCGGCGAAGCCGGTGCTCGCGGGCACGCCCGCTCCCACATCGATAATGCATGCCTTTACCGTACACCGGCCGAGCGCGTAATCTCCGGCCATGCCACATATCGCCCGTCTGtt                                           >  5:341641/5‑119 (MQ=39)
                                                                                                                                                ttCGCCGGTGTTCGCGGGCACGCCCGCTCCCACATCGATAATGCATGCCTTTACCGTACACCGGCCGAGCGCGTAATCTCCGGCCATGCCACATATCGCCCGTCTGTTCTGGATCCTGCTGCTCGTTTGCCTGAGCCCGTTGGCTCTgg  >  6:336209/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                                   
TCGCTGGGCGAGCGGCGCAAGGATATCGAGGCGCATCTGCTGCGATCCACCCAAGCCTGATGTGCCCGCAAAACTCATTGTCTTGCGCGGTCTTGTGGGAGCGGGCGTGCCCGCGAAGAGAGCAACCCGATCGTTGGCACCGGCTTCGCCGGTGTTCGCGGGCACGCCCGCTCCCACATCGATAATGCATGCCTTTACCGTACACCGGCCGAGCGCGTAATCTCCGGCCATGCCACATATCGCCCGTCTGTTCTGGATCCTGCTGCTCGTTTGCCTGAGCCCGTTGGCTCTGG  >  NC_002947/5671522‑5671814

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑