Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,674,397 C→G 5.8% P547R (CCG→CGG)  rhlE‑II → ATP‑dependent RNA helicase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,674,3970CG 5.8% 214.4 / 2.0 67P547R (CCG→CGG) rhlE‑IIATP‑dependent RNA helicase
Reads supporting (aligned to +/- strand):  ref base C (32/31);  new base G (2/2);  total (34/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.79e-01

ACCAGGGCAAGAACCAGGGCCGCAACCGCCGCCCGGGTGGTGCTGGCCAAGGTCAGGGGCAAGGCAATGGCCAACGCAGCAACGCTGGCGGCCAGGGCCAAGCCCGCAATGGCGGCCAGCCACGCAGTGGTGGCGGAGAAAAACGCCCGCCCCGCGCCAACAACGGTGGCGGTGCCCGTCGTGATGGCGGCGGTGGCCGTGGTCGCCCTGCGCGTGACGATGCTG‑CCCGGCAAGAGCCGGCCGTGCGCAACCCGCGTCAGCCGGAAAAACAGCCAGTGATCATTCGCAAG  >  NC_002947/5674250‑5674539
                                                                                                                                                   |                                                                                                                                               
aCCAGGGCAAGAACCAGGGCCGCAACCGCCGCCCGGGTGGTGCTGGCCAAGGTCAGGGGCAAGGCAATGGCCAACGCAGCAACGCTGGCGGCCAGGGCCAAGCCCGCAATGGCGGCCAGCCACGCAGTGGTGGCGGAGAAAAAcgcccg                                                                                                                                                >  3:310092/1‑149 (MQ=255)
         aGAACCAGGGCCGCAACCGCCGCCCGGGTGATGCTGGCCAAGGTCAGGGGCAAGGCAATGGCCAACGCAGCAACGCTGGCGGCCAGGGCCAAGCCCGCAATGGCGGCCAGCCACGCAGTGGTGGCGGAGAAAAACGCCCGCCCCgcgc                                                                                                                                        >  8:198858/1‑148 (MQ=255)
             ccAGGGCCGCAACCGCCGCCCGGGTGGTGCTGGCCAAGGTCAGGGGCAAGGCAATGGCCAACGCAGCAACGCTGGCGGCCAGGGCCAAGCCCGCAATGGCGGCCAGCCACGCAGTGGTGGCGGAGAAAAACGCCCGCCCCGCGCcaac                                                                                                                                    >  5:353487/1‑148 (MQ=255)
                    cGCAACCGCCGCCCGGGTGGTGCTGGCCAAGGTCAGGGGCAAGGCTATGGCCAACGCAGCAACGCTGGCGGCCAGGGCCAAGCCCGCAATGGCAGCCAGCCACGCAGTGGTGGCGGAGAAAAACGCCCGCCCCGCGCCAACAACGGTgg                                                                                                                            <  5:296710/149‑1 (MQ=255)
                    cGCAACCGCCGCCCGGGTGGTGCTGGCCAAGGTCAGGGGCAAGGCAATGGCCAACGCAGCAACGCTGGCGGCCAGGGCCAAGCCCGCAATGGCGGCCAGCCACGCAGTGGTGGCGGAGAAAAACGCCCGCCCCGCGCCAACAACGGTgg                                                                                                                            >  3:235401/1‑149 (MQ=255)
                      cAACCGCCGCCCGGGTGGTGCTGGCCAAGGTCAGGGGCAAGGCAATGGCCAACGCAGCAACGCTGGCGGCCAGGGCCAAGCCCGCAATGGCGGCCAGCCACGCAGTGGTGGCGGAGAAAAACGCCCGCCCCGCGCCAACAACGGTGGCg                                                                                                                          >  6:14338/1‑149 (MQ=255)
                          cgccgcCCGGGTGGTGCTGGCCAAGGTCAGGGGCAAGGCAATGGCCAACGCAGCAACGCTGGCGGCCAGGGCCAAGCCCGCAATGGCGGCCAGCCACGCAGTGGTGGCGGAGAAAAACGCCCGCCCCGCGCCAACAACGGTGGCGGTGc                                                                                                                      <  4:235401/149‑1 (MQ=255)
                                        tgCTGGCCAAGGTCAGGGGCAAGGCAATGGCCAACGCAGCAACGCTGGCGGCCAGGGCCAAGCCCGCAATGGCGGCCAGCCACGCAGTGGTGGCGGAGAAAAACGCCCGCCCCGCGCCAACAACGGTGGCGGTGCCCGTCGTGATggcg                                                                                                        >  8:206015/1‑149 (MQ=255)
                                          cTGGCCAAGGTCAGGGGCAAGGCAATGGCCAACGCAGCAACGCTGGCGGCCAGGGCCAAGCCCGCAATGGCGGCCAGCCACGCAGTGGTGGCGGAGAAAAACGCCCGCCCCGCGCCAACAACGGTGGCGGTGCCCGTCGTGATggcgg                                                                                                       <  4:227125/148‑1 (MQ=255)
                                          cTGGCCAAGGTCAGGGGCAAGGCAATGGCCAACGCAGCAACGCTGGCGGCCAGGGCCAAGCCCGCAATGGCGGCCAGCCACGCAGTGGTGGCGGAGAAAAACGCCCGCCCCGCGCCAACAACGGTGGCGGTGCCCGTCGTGATggcgg                                                                                                       <  6:175185/148‑1 (MQ=255)
                                            ggCCAAGGTCAGGGGCAAGGCAATGGCCAACGCAGCAACGCTGGCGGCCAGGGCCAAGCCCGCAATGGCGGCCAGCCACGCAGTGGTGGCGGAGAAAAACGCCCGCCCCGCGCCAACAACGGTGGCGGTGCCCGTCGTGATGGcggcgg                                                                                                    >  4:98105/1‑149 (MQ=255)
                                               cAAGGTCAGGGGCAAGGCAATGGCCAACGCAGCAACGCTGGCGGCCAGGGCCAAGCCCGCAATGGCGGCCAGCCACGCAGTGGTGGCGGAGAAAAACGCCCGCCCCGCGCCAACAACGGTGGCGGTGCCCGTCGTGATGGCGGCGGTgg                                                                                                 <  8:200426/149‑1 (MQ=255)
                                                  ggTCAGGGGCAAGGCAATGGCCAACGCAGCAACGCTGGCGGCCAGGGCCAAGCCCGCAATGGCGGCCAGCCACGCAGTGGTGGCGGAGAAAAACGCCCGCACCGCGCCAACCACGGGGGCGGTGCACGTCGTGATGGCGGCGGTCGCCg                                                                                              >  4:262550/1‑149 (MQ=255)
                                                          gcaaggcaaTGGCCAACGCAGCAACGCTGGCGGCCAGGGCCAAGCCCGCAATGGCGGCCAGCCACGCAGTGGTGGCGGAGAAAAACGCCCGCCCCGCGCCAACAACGGTGGCGGTGCCCGTCGTGATGGCGGCGGTGGCCGTGGTCGcc                                                                                      >  1:304083/1‑149 (MQ=255)
                                                                  aTGGCCAACGCAGCAACGCTGGCGGCCAGGGCCAAGCCCGCAATGGCGGCCAGCCACGCAGTGGTGGCGGAGAAAAACGCCCGCCCCGCGCCAACAACGGTGGCGGTGCCCGTCGTGATGGCGGCGGTGGCCGTGGTCGCCCTGCGCGt                                                                              <  5:320355/149‑1 (MQ=255)
                                                                    ggCCAACGCAGCAACGCTGGCGGCCAGGGCCAAGCCCGCAATGGCGGCCAGCCACGCAGTGGTGGCGGAGAAAAACGCCCGCCCCGCGCCAACAACGGTGGCGGTGCCCGTCGTGATGGCGGCGGTGGCCGTGGTCGCCCTGCGCGTGa                                                                            >  4:31305/1‑149 (MQ=255)
                                                                     gCCAACGCAGCAACGCTGGCGGCCAGGGCCAAGCCCGCAATGGCGGCCAGCCACGCAGTGGTGGCGGAGAAAAACGCCCGCCCCGCGCCAACAACGGTGGCGGTGCCCGTCGTGATGGCGGCGGTGGCCGTGGTCGCCCTGCGCGTGa                                                                            <  3:253717/148‑1 (MQ=255)
                                                                         aCGCAGCAACGCTGGCGGCCAGGGCCAAGCCCGCAATGGCGGCCAGCCACGCAGTGGTGGCGGAGAAAAACGCCCGCCCCGCGCCAACAACGGTgg                                                                                                                            >  7:102351/1‑96 (MQ=255)
                                                                         aCGCAGCAACGCTGGCGGCCAGGGCCAAGCCCGCAATGGCGGCCAGCCACGCAGTGGTGGCGGAGAAAAACGCCCGCCCCGCGCCAACAACGGTgg                                                                                                                            <  8:102351/96‑1 (MQ=255)
                                                                         aCGCAGCAACGCTGGCGGCCAGGGCCAAGCCCGCAATGGCGGCCAGCCACGCAGTGGTGGCGGAGAAAAACGCCCGCCCCGCGCCAACAACGGTGGCGGTGCCCGTCGTGATGGCGGCGGTGGCCGTGGTCGCCCTGCGCGTGACGAtg                                                                       >  2:75320/1‑149 (MQ=255)
                                                                         aCGCAGCAACGCTGGCGGCCAGGGCCAAGCCCGCAATGGCGGCCAGCCACGCAGTGGTGGCGGAGAAAAACGCCCGCCCCGCGCCAACAACGGTGGCGGTGCCCGTCGTGATGGCGGCGGTGGCCGTGGTCGCCCTGCGCGTGACGAtg                                                                       <  3:98105/149‑1 (MQ=255)
                                                                           gcagcaACGCTGGCGGCCAGGGCCAAGCCCGCAATGGCGGCCAGCCACGCAGTGGTGGCGGAGAAAAACGCCCGCCCCGCGCCAACAACGGTGGCGGTGCCCGTCGTGATGGCGGCGGTGGCCg                                                                                              >  4:358255/1‑124 (MQ=255)
                                                                           gcagcaACGCTGGCGGCCAGGGCCAAGCCCGCAATGGCGGCCAGCCACGCAGTGGTGGCGGAGAAAAACGCCCGCCCCGCGCCAACAACGGTGGCGGTGCCCGTCGTGATGGCGGCGGTGGCCg                                                                                              <  3:358255/124‑1 (MQ=255)
                                                                               caACGCTGGCGGCCAGGGCCAAGCCCGCAATGGCGGCCAGCCACGCAGTGGTGGCGGAGAAAAACGCCCGCCCCGCGCCAACAACGGTGGCGGTGCCCGTCGTGATGGCGGCGGTGGCCGTGGTCGCCCTGCGCGTGACGATGCTG‑ccc                                                                >  7:292177/1‑149 (MQ=255)
                                                                                  cGCTGGCGGCCAGGGCCAAGCCCGCAATGGCGGCCAGCCACGCAGTGGTGGCGGAGAAAAACGCCCGCCCCGCGCCAACAACGGTGGCGGTGCCCGTCGTGATGGCGGCGGTGGCCGTGGTCGCCCTGCGCGTGACGATGCTG‑CCCGGc                                                             <  7:206015/149‑1 (MQ=255)
                                                                                       gcggcCAGGGCCAAGCCCGCAATGGCGGCCAGCCACGCAGTGGTGGCGGAGAAAAACGCCGGCCCCGCGCCAACAACGGTGGCGGTGCCCGTCGTGATGGCGGCGGTGGCCGTGGTCGCCCTGCGCGTGACGATGCTG‑CCCGGCAagag                                                        >  4:134650/1‑149 (MQ=255)
                                                                                       gcggcCAGGGCCAAGCCCGCAATGGCGGCCAGCCACGCAGTGGTGGCGGAGAAAAACGCCCGCCCCGCGCCAACAACGGTGGCGGTGCCCGTCGTGATGGCGGCGGTGGCCGTGGTCGCCCTGCGCGTGACGATGCTG‑CCCGGCAagag                                                        <  3:288447/149‑1 (MQ=255)
                                                                                       gcggcCAGGGCCAAGCCCGCAATGGCGGCCAGCCACGCAGTGGTGGCGGAGAAAAACGCCCGCCCCGCGCCAACAACGGTGGCGGTGCCCGTCGTGATGGCGGCGGTGGCCGTGGTCGCCCTGCGCGTGACGATGCTG‑CCCGGCAagag                                                        >  2:64013/1‑149 (MQ=255)
                                                                                       gcggcCAGGGCCAAGCCCGCAATGGCGGCCAGACACGCAGTGGTGGCGGAGAAAAACGCCGGCCCCGCGCCAACAACGGTGGCGGTGCCCGTCGTGATGGCGGCGGTGGCCGTGGTCGCCCTGCGCGTGACGATGCTG‑CCCGGCAagag                                                        >  2:96862/1‑149 (MQ=255)
                                                                                          gcCAGGGCCAAGCCCGCAATGGCGGCCAGCCACGCAGTGGTGGCGGAGAAAAACGCCCGCCCCGCGCCAACAACGGTGGCGGTGCCCGTCGTGATGGCGGCGGTGGCCGTGGTCGCCCTGCGCGTGACGATGCTG‑CCCGGCAAGAgccg                                                     >  3:89741/1‑149 (MQ=255)
                                                                                           ccAGGGCCAAGCCCGCAATGGCGGCCAGCCACGCAGTGGTGGCGGAGAAAAACGCCCGCCCCGCGCCAACAACGGTGGCGGTGCCCGTCGTGATGGCGGCGGTGGCCGTGGTCGCCCTGCGCGTGACGATGCTG‑CCCGGCAaga                                                         <  3:213239/144‑1 (MQ=255)
                                                                                           ccAGGGCCAAGCCCGCAATGGCGGCCAGCCACGCAGTGGTGGCGGAGAAAAACGCCCGCCCCGCCCCAACAACGGTGGCGGTGCCCGTCGTGATGGCGGCGGTGGCCGTGGTCGCCCTGCGCGTGACGATGCTG‑CCCGGCAaga                                                         >  4:213239/1‑144 (MQ=255)
                                                                                             aGGGCCAAGCCCGCAATGGCGGCCAGCCACGCAGTGGTGGCGGAGAAAAACGCCCGCCCCGCGCCAACAACGGTGGCGGTGCCCGTCGTGATGGCGGCGGTGGCCGTGGTCGCCCTGCGCGTGACGATGCTG‑CCCGGCAAGAgccggcc                                                  >  4:337624/1‑149 (MQ=255)
                                                                                                 ccAAGCCCGCAATGGCGGCCAGCCACGCAGTGGTGGCGGAGAAAAACGCCCGCCCCGCGCCAACAACGGTGGCGGTGCCCGTCGTGATGGCGGCGGTGGCCGTGGTCGCCCTGCGCGTGACGATGCTG‑CCCGGCAAGAGCCGGCCGTgc                                              >  8:227119/1‑149 (MQ=255)
                                                                                                 ccAAGCCCGCAATGGCGGCCAGCCACGCAGTGGTGGCGGAGAAAAACGCCCGCCCCGCGCCAACAACGGTGGCGGTGCCCGTCGTGATGGCGGCGGTGGCCGTGGTCGCCCTGCGCGTGACGATGCTG‑CCCGGCAAGAGCCGGCCGTgc                                              >  5:204274/1‑149 (MQ=255)
                                                                                                 ccAAGCCCGCAATGGCGGCCAGCCACGCAGTGGTGGCGGAGAAAAACGCCCGCCCCGCGCCAACAACGGTGGCGGTGCCCGTCGTGATGGCGGCGGTGGCCGTGGTCGCCCTGCGCGTGACGATGCTG‑CCCGGCAAGAGCCGGCCGTgc                                              >  2:277386/1‑149 (MQ=255)
                                                                                                  cAAGCCCGCAATGGCGGCCAGCCACGCAGTGGTGGCGGAGAAAAACGCCCGCCCCGCGCCAACAACGGTGGCGGTGCCCGTCGTGATGGCGGCGGTGGCCGTGGTCGCCCTgc                                                                                  >  6:15218/1‑113 (MQ=255)
                                                                                                  cAAGCCCGCAATGGCGGCCAGCCACGCAGTGGTGGCGGAGAAAAACGCCCGCCCCGCGCCAACAACGGTGGCGGTGCCCGTCGTGATGGCGGCGGTGGCCGTGGTCGCCCTgc                                                                                  <  5:15218/113‑1 (MQ=255)
                                                                                                   aaGCCCGCAATGGCGGCCAGCCACGCAGTGGTGGCGGAGAAAAACGCCGGCCCCGCGCCAACAACGGTGTCGGTGCCCGTCGTGATGGCGGCGGTGGCCGTGGTCGCCCTGCGCGTGACGATGCTG‑CCCGGCAAGAGCCGGCCGTgcgc                                            <  1:96862/149‑1 (MQ=255)
                                                                                                   aaGCCCGCAATGGCGGCCAGCCACGCAGTGGTGGCGGAGAAAAACGCCGGCCCCGCGCCAACAACGGTGGCGGTGCCCGTCGTGATGGCGGCGGTGGCCGTGGTCGCCCTGCGCGTGACGATGCTG‑CCCGGCAAGAGCCGGCCGTgcgc                                            <  3:134650/149‑1 (MQ=255)
                                                                                                        cGCAATGGCGGCCAGCCACGCAGTGGTGGCGGAGAAAAACGCCCGCCCCGCGCCAACAACGGTGGCGGTGCCCGTCGTGATGGCGGCGGTGGCCGTGGTCGCCCTGCGCGt                                                                              >  4:259500/1‑111 (MQ=255)
                                                                                                        cGCAATGGCGGCCAGCCACGCAGTGGTGGCGGAGAAAAACGCCCGCCCCGCGCCAACAACGGTGGCGGTGCCCGTCGTGATGGCGGCGGTGGCCGTGGTCGCCCTGCGCGt                                                                              <  3:259500/111‑1 (MQ=255)
                                                                                                        cGCAATGGCGGCCAGCCACGCAGTGGTGGCGGAGAAAAACGCCCGCCCCGCGCCAACAACGGTGGCGGTGCCCGTCGTGATGGCGGCGGTGGCCGTGGTCGCCCTGCGCGTGACGATGCTG‑CCCGGCAAGAGCCGGCCGTGCGCAAccc                                       >  8:302163/1‑149 (MQ=255)
                                                                                                            aTGGCGGCCAGCCACGCAGTGGTGGCGGAGAAAAACGCCCGCCCCGCGCCAACAACGGTGGCGGTGCCCGTCGTGATGGCGGCGGTGGCCGTGGTCGCCCTGCGCGTGACGATGCTG‑CCCGGCAAGAGCCGGCCGTGCGCAACCCGCGt                                   <  1:277386/149‑1 (MQ=255)
                                                                                                            aTGGCGGCCAGCCACGCAGTGGTGGCGGAGAAAAACGCCCGCCCCGCGCCAACAACGGTGGCGGTGCCCGTCGTGATGGCGGCGGTGGCCGTGGTCGCCCTGCGCGTGACGATGCTG‑CCCGGCAAGAGCCGGCCGTGCGCAACCCGCGt                                   <  4:310092/149‑1 (MQ=255)
                                                                                                             tGGCGGCCAGCCACGCAGTGGTGGCGGAGAAAAACGCCCGCCCCGCGCCAACAACGGTGGCGGTGCCCGTCGTGATGGCGGCGGTGGCCGTGGTCGCCCTGCGCGTGACGATGCTG‑CCCGGCAAGAGCCGGCCGTGCGCAACCCGCGTc                                  <  8:321548/149‑1 (MQ=255)
                                                                                                                  gccagccaCGCAGTGGTGGCGGAGAAAAACGCCCGCCCCGCGCCAACAACGGTGGCGGTGCCCGTCGTGATGGCGGCGGTGGCCGTGGTCGCCCTGCGCGTGACGATGCTGCCCCGGCAAGAGCCGGCCGTGCGCAACCCGCGTCAGcc                              <  1:64013/149‑1 (MQ=255)
                                                                                                                    cagccaCGCAGTGGTGGCGGAGAAAAACGCCCGCCCCGCGCCAACAACGGTGGCGGTGCCCGTCGTGATGGCGGCGGTGGCCGTGGTCGCCCTGCGCGTGACGATGCTG‑CCCGGCAAGAGCCGGCCGTGCGCAACCCGCGTCAGCCGGa                           <  3:31305/149‑1 (MQ=255)
                                                                                                                     agccaCGCAGTGGTGGCGGAGAAAAACGCCCGCCCCGCGCCAACAACGGTGGCGGTGCCCGTCGTGATGGCGGCGGTGGCCGTGGTCGCCCTGCGCGTCACGATGCTG‑CCCGGCAAGAGCCGGCCGTGCGCAACCCGCGTCAGCCGGa                           >  4:194147/1‑148 (MQ=255)
                                                                                                                      gccaCGCAGTGGTGGCGGAGAAAAACGCCCGCCCCGCGCCAACAACGGTGGCGGTGCCCGTCGTGATGGCGGCGGTGGCCGTGGTCGCCCTGCGCGTGACGATGCTG‑CCCGGCAAGAGCCGGCCGTGCGCAACCCGCGTCAGCCGGaa                          >  7:204450/1‑148 (MQ=255)
                                                                                                                         aCGCAGTGGTGGCGGAGAAAAACGCCCGCCCCGCGCCAACAACGGTGGCGGTGCCCGTCGTGATGGCGGCGGTGGCCGTGGTCGCCCTGCGCGTGACGATGCTG‑CCCGGCAAGAGCCGGCCGTGCGCAACCCGCGTCAGCCGGAAAAAc                      <  7:198858/149‑1 (MQ=255)
                                                                                                                         aCGCAGTGGTGGCGGAGAAAAACGCCCGCCCCGCGCCAACAACGGTGGCGGTGCCCGTCGTGATGGCGGCGGTGGCCGTGGTCGCCCTGCGCGTGACGATGCTG‑CCCGGCAAGAGCCGGCCGTGCGCAACCCGCGTCAGCCGGAAAAAc                      <  2:304083/149‑1 (MQ=255)
                                                                                                                          cGCAGTGGTGGCGGAGAAAAACGCCCGCCCCGCGCCAACAACGGTGGCGGTGCCCGTCGTGATGGCGGCGGTGGCCGTGGTCGCCCTGCGCGTGACGATGCTG‑CCCGGCAAGAGCCGGCCGTGCGCAACCCGCGTCAGCCGGAAAAACa                     <  5:14338/149‑1 (MQ=255)
                                                                                                                           gCAGTGGTGGCGGAGAAAAACGCCCGCCCCGCGCCAACAACGGTGGCGGTGCCCGTCGTGATGGCGGCGGTGGCCGTGGTCGCCCTGCGCGTGACGATGCTG‑CCCGGCAAGAGCCGGCCGTGCGCAACCCGCGTCAGCCGGAAAAACAg                    <  7:227119/149‑1 (MQ=255)
                                                                                                                                 gtggCGGAGAAAAACGCCCGCCCCGCGCCAACAACGGTGGCGGTGCCCGTCGTGATGGCGGCGGTGGCCGTGGTCGCCCTGCGCGTGACGATGCTG‑CCCGGCAAGAGCCGGCCGTGCGCAACCCGCGTCAGCCGGAAAAACAGCCAGt               <  2:96590/148‑1 (MQ=255)
                                                                                                                                  tggCGGAGAAAAACGCCCGCCCCGCGCCAACAACGGTGGCGGTGCCCGTCGTGATGGCGGCGGTGGCCGTGGTCGCCCTGCGCGTGACGATGCTG‑CCCGGCAAGAGCCGGCCGTGCGCAACCCGCGTCAGCCGGAAAAACAGCCAGTGa             <  6:204274/149‑1 (MQ=255)
                                                                                                                                    gCGGAGAAAAACGCCCGCCCCGCGCCAACAACGGTGGCGGTGCCCGTCGTGATGGCGGCGGTGGCCGTGGTCGCCCTGCGCGTGACGATGCTG‑CCCGGCAAGAGCCGGCCGTGCGCAACCCGCGTCAGCCGGAAAAACAGCCAGTGAt            >  8:260153/1‑148 (MQ=255)
                                                                                                                                      ggAGAAAAACGCCCGCCCCGCGCCAACAACGGTGGCGGTGCCCGTCGTGATGGCGGCGGTGGCCGTGGTCGCCCTGCGCGTGACGATGCTG‑CCCGGCAAGAGCCGGCCGTGCGCAACCCGCGTCAGCCGGAAAAACAGCCAGTGATCAt         <  3:194147/149‑1 (MQ=255)
                                                                                                                                      ggAGAAAAACGCCCGCCCCGCGCCAACAACGGTGGCGGTGCCCGTCGTGATGGCGGCGGTGGCCGTGGTCGCCCTGCGCGTGACGATGCTG‑CCCGGCAAGAGCCGGCCGTGCGCAACCCGCGTCAGCCGGAAAAACAGCCAGTGATCAt         >  8:172352/1‑149 (MQ=255)
                                                                                                                                        agaAAAACGCCCGCCCCGCGCCAACAACGGTGGCGGTGCCCGTCGTGATGGCGGCGGTGGCCGTGGTCGCCCTGCGCGTGACGATGCTG‑CCCGGCAAGAGCCGGCCGTGCGCAACCCGCGTc                                  <  2:173154/122‑1 (MQ=255)
                                                                                                                                        agaAAAACGCCCGCCCCGCGCCAACAACGGTGGCGGTGCCCGTCGTGATGGCGGCGGTGGCCGTGGTCGCCCTGCGCGTGACGATGCTG‑CCCGGCAAGAGCCGGCCGTGCGCAACCCGCGTc                                  >  1:173154/1‑122 (MQ=255)
                                                                                                                                         gaAAAACGCCCGCCCCGCGCCAACAACGGTGGCGGTGCCCGTCGTGATGGCGGCGGTGGCCGTGGTCGCCCTGCGCGTGACGATGCTG‑CCCGGCAAGAGCCGGCCGTGCGCAACCCGCGTCAGCCGGaaa                         <  5:274012/130‑1 (MQ=255)
                                                                                                                                         gaAAAACGCCCGCCCCGCGCCAACAACGGTGGCGGTGCCCGTCGTGATGGCGGCGGTGGCCGTGGTCGCCCTGCGCGTGACGATGCTG‑CCCGGCAAGAGCCGGCCGTGCGCAACCCGCGTCAGCCGGaaa                         >  6:274012/1‑130 (MQ=255)
                                                                                                                                         gaAAAACGCCCGCCCCGCGCCAACAACGGTGGCGGTGCCCGTCGTGATGGCGGCGGTGGCCGTGGTCGCCCTGCGCGTGACGATGCTG‑CCCGGCAAGAGCCGGCCGTGCGCAACCCGCGTCAGCCGGAAAAACAGCCAGTGATCATTCg      <  7:302163/149‑1 (MQ=255)
                                                                                                                                            aaaCGCCCGCCCCGCGCCAACAACGGTGGCGGTGCCCGTCGTGATGGCGGCGGTGGCCGTGGTCGCCCTGCGCGTGACGATGCTG‑CCCGGCAAGAGCCGGCCGTGCGCACCCCGCGTCAGCCGGAAAAACAGCCAGTGATCATTCGc     >  2:258449/1‑147 (MQ=255)
                                                                                                                                            aaaCGCCCGCCCCGCGCCAACAACGGTGGCGGTGCCCGTCGTGATGGCGGCGGTGGCCGTGGTCGCCCTGCGCGTGACGATGCTG‑CCCGGCAAGAGCCGGCCGTGCGCAACCCGCGTCAGCCGGAAAAACAGCCAGTGATCATTCGc     >  5:201251/1‑147 (MQ=255)
                                                                                                                                            aaaCGCCCGCCCCGCGCCAACAACGGTGGCGGTGCCCGTCGTGATGGCGGCGGTGGCCGTGGTCGCCCTGCGCGTGACGATGCTG‑CCCGGCAAGAGCCGGCCGTGCGCAACCCGCGTCAGCCGGAAAAACAGCCAGTGATCATTCGCaa   <  8:204450/149‑1 (MQ=255)
                                                                                                                                             aaCGCCCGCCCCGCGCCAACAACGGTGGCGGTGCCCGTCGTGATGGCGGCGGTGGCCGTGGTCGCCCTGCGCGTGACGATGCTG‑CCCGGCAAGAGCCGGCCGTGCGCAACCCGCGTCAGCCGGAAAAACAGCCAGTGATCATTCGCAAg  >  4:55413/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                               
ACCAGGGCAAGAACCAGGGCCGCAACCGCCGCCCGGGTGGTGCTGGCCAAGGTCAGGGGCAAGGCAATGGCCAACGCAGCAACGCTGGCGGCCAGGGCCAAGCCCGCAATGGCGGCCAGCCACGCAGTGGTGGCGGAGAAAAACGCCCGCCCCGCGCCAACAACGGTGGCGGTGCCCGTCGTGATGGCGGCGGTGGCCGTGGTCGCCCTGCGCGTGACGATGCTG‑CCCGGCAAGAGCCGGCCGTGCGCAACCCGCGTCAGCCGGAAAAACAGCCAGTGATCATTCGCAAG  >  NC_002947/5674250‑5674539

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑