| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,696,584 | A→G | 13.0% | intergenic (+36/‑176) | pyrC' → / → hslV | dihydroorotase‑like protein/ATP‑dependent HslVU protease peptidase subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,696,584 | 0 | A | G | 13.0% | 112.4 / 8.8 | 46 | intergenic (+36/‑176) | pyrC'/hslV | dihydroorotase‑like protein/ATP‑dependent HslVU protease peptidase subunit |
| Reads supporting (aligned to +/- strand): ref base A (15/25); new base G (2/4); total (17/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.54e-01 | |||||||||||
CTGGCGAACAATGGTTCTCCCGTGGCGAGAACTGCCCGTTCATCGGCCACTGCCTGCCGGGCGCGGTGCGTTATACCTTGGTCGATGGCCACATCTGCCACGAGGCCTGAGTACCATGCTTAGCGGGCACGCCCGCTCCCACAGGATCACCACAATTCCAAGGCA‑TGTGGTAACCCTGTGGGAGCGGGCATGCCCGCGAATGCCTTCACATAGTCCCCCCGCCAAGCGCGACCATTCATCCCCCCCGGTTGAAATCCTTCCCCCCACCCCCATATGAGTCT > NC_002947/5696439‑5696719 | cTGGCGAACAATGGTTCTCCCGTGGCGAGAACTGCCCGTTCATCGGCCACTGCCTGCCGGGCGCGGTGCGTTATACCTTGGTCGATGGCCACATCTGCCACGAGGCCTGAGTACCATGCTTAGCGGGCACGCCCGCTCCCACAGGATca < 6:21374/149‑1 (MQ=255) tGGCGAACAATGGTTCTCCCGTGGCGAGAACTGCCCGTTCATCGGCCACTGCCTGCCGGGCGCGGTGCGTTATACCTTGGTCGATGGCCACATCTGCCACGAGGCCTGAGTACCATGCTTAGCGGGCACGCCCGCTCCCACAGGATcac > 4:69260/1‑149 (MQ=255) gCGAACAATGGTTCTCCCGTGGCGAGAACTGCCCGTTCATCGGCCACTGCCTGCCGGGCGCGGTGCGTTATACCTTGGTCGATGGCCACATCTGCCACGAGGCCTGAGTACCATGCTTAGCGGGCACGCCCGCTCCCACAGGATcacca < 8:166456/149‑1 (MQ=255) gCGAACAATGGTTCTCCCGTGGCGAGAACTGCCCGTTCATCGGCCACTGCCTGCCGGGCGCGGTGCGTTATACCTTGGTCGATGGCCACATCTGCCACGAGGCCTGAGTACCATGCTTAGCGGGCACGCCCGCTCCCACAGGATcacca > 6:94356/1‑149 (MQ=255) cGAACAATGGTTCTCCCGTGGCGAGAACTGCCCGTTCATCGGCCACTGCCTGCCGGGCGCGGTGCGTTATACCTTGGTCGATGGCCACATCTGCCACGAGGCCTGAGTACCATGCTTAGCGGGCATGCCCGCTCCCACAGGGTtacca > 2:297356/1‑148 (MQ=255) cGAACAATGGTTCTCCCGTGGCGAGAACTGCCCGTTCATCGGCCACTGCCTGCCGGGCGCGGTGCGTTATACCTTGGTCGATGGCCACATCTGCCACGAGGCCTGAGTACCATGCTTAGCGGGCATGCCCGCTCCCACAGGGTtacca > 2:293240/1‑148 (MQ=255) cGAACAATGGTTCTCCCGTGGCGAGAACTGCCCGTTCATCGGCCACTGCCTGCCGGGCGCGGTGCGTTATACCTTGGTCGATGGCCACATCTGCCACGAGGCCTGAGTACCATGCTTAGCGGGCACGCCCGCTCCCACAGGATcaccac < 2:286235/149‑1 (MQ=255) gAACAATGGTTCTCCCGTGGCGAGAACTGCCCGTTCATCGGCCACTGCCTGCCGGGCGCGGTGCGTTATACCTTGGTCGATGGCCACATCTGCCACGAGGCCTGAGTACCATGCTTAGCGGGCACGCCCGCTCCCACAGGATcaccac > 6:13655/1‑148 (MQ=255) cAATGGTTCTCCCGTGGCGAGAACTGCCCGTTCATCGGCCACTGCCTGCCGGGCGCGGTGCGTTATACCTTGGTCGATGGCCACATCTGCCACGAGGCCTGAGTACCATGCTTAGCGGGCACGCCCGCTCCCACAGGATCACCACAAtt < 2:337061/149‑1 (MQ=255) aaTGGTTCTCCCGTGGCGAGAACTGCCCGTTCATCGGCCACTGCCTGCCGGGCGCGGTGCGTTATACCTTGGTCGATGGCCACATCTGCCACGAGGCCTGAGTACCATGCTTAGCGGGCACGCCCGCTCCCACAGGATCACCACAATTc > 4:82775/1‑149 (MQ=255) aaTGGTTCTCCCGTGGCGAGAACTGCCCGTTCATCGGCCACAGCCTGCCGGGCGCGGTGCGTTATACCTTGGTCGATGGCCACATCTGCCACGAGGCCTGAGTACCATGCTTAGCGGGCACGCCCGCTCCCACAGGATCACCACAATTc > 7:92870/1‑149 (MQ=255) gctctcCCGGGGCGAGAACTGCCCGTTCCTCGGCCACTGCCTGCCGGGCGCGGTGCGATATACCTTTGTCGATGGCCACATCTGCCACGAGGCCTGAGTACCATGCTTAGCGGGCACGCCCGCTCCCACAGGATCACCACAATTCCAAg < 8:288730/147‑1 (MQ=255) ctcCCGTGGCGAGAACTGCCCGTTCATCGGCCACTGCCTGCCGGGCGCGGTGCGTTATACCTTGGTCGATGGCCACATCTGCCACGAGGCCTGAGTACCATGCTTAGCGGGCACGCCCGCTCCCACAGGATCACCACAATTCCAAGGc < 3:82775/148‑1 (MQ=255) ccGTGGCGAGAACTGCCCGTTCATCGGCCACTGCCTGCCGGGCGCGGTGCGTTATACCTTGGTCGATGGCCACATCTGCCACGAGGCCTGAGTACCATGCTTAGCGGGCACGCCCGCTCCCACAGGATCACCACAATTCCAAGGCA‑tgt > 4:313495/1‑149 (MQ=255) ccGTGGCGAGAACTGCCCGTTCATCGGCCACTGCCTGCCGGGCGCGGTGCGTTATACCTTGGTCGATGGCCACATCTGCCACGAGGCCTGAGTACCATGCTTAGCGGGCACGCCCGCTCCCACAGGATCACCACAATTCCAAGGCA‑tg < 3:163901/148‑1 (MQ=255) ccGTGGCGAGAACTGCCCGTTCATCGGCCACTGCCTGCCGGGCGCGGTGCGTTATACCTTGGTCGATGGCCACATCTGCCACGAGGCCTGAGTACCATGCTTAGCGGGCACGCCCGCTCCCACAGGATCACCACAATTCCAAGGCA‑agt > 2:208206/1‑149 (MQ=255) gTGGCGAGAACTGCCCGTTCATCGGCCACTGCCTGCCGGGCGCGGTGCGTTATACCTTGGTCGATGGCCACATCTGCCACGAGGCCTGAGTACCATGCTTAGCGGGCACGCCCGCTCCCACAGGATCACCACAATTCCAAGGCA‑TGTgg > 3:181830/1‑149 (MQ=255) gCGAGAACTGCCCGTTCATCGGCCACTGCCTGCCGGGCGCGGTGCGTTATACCTTGGTCGATGGCCACATCTGCCACGAGGCCTGAGTACCATGCTTAGCGGGCACGCCCGCTCCCACAGGATCACCACAATTCCAAGGCA‑TGTGGTaa < 5:94356/149‑1 (MQ=255) aCTGCCCGTTCATCGGCCACTGCCTGCCGGGCGCGGTGCGTTATACCTTGGTCGATGGCCACATCTGCCACGAGGCCTGAGTACCATGCTTAGCGGGCACGCCCGCTCCCACAGGATCACCACAATTCCAAGGCA‑TGTGGTAACCCtgt < 2:274228/149‑1 (MQ=255) aCTGCCCGTTCATCGGCCACTGCCTGCCGGGCGCGGTGCGTTATACCTTGGTCGATGGCCACATCTGCCACGAGGCCTGAGTACCATGCTTAGCGGGCACGCCCGCTCCCACAGGATCACCACAATTCCAAGGCA‑TGTGGTAACCCtgt < 8:123891/149‑1 (MQ=255) cTGCCCGTTCATCGGCCACTGCCTGCCGGGCGCGGTGCGTTATACCTTGGTCGATGGCCACATCTGCCACGAGGCCTGAGTACCATGCTTAGCGGGCACGCCCGCTCCCACAGGATCACCACAATTCCAAGGCA‑tg < 2:258278/136‑1 (MQ=255) cTGCCCGTTCATCGGCCACTGCCTGCCGGGCGCGGTGCGTTATACCTTGGTCGATGGCCACATCTGCCACGAGGCCTGAGTACCATGCTTAGCGGGCACGCCCGCTCCCACAGGATCACCACAATTCCAAGGCA‑tg > 1:258278/1‑136 (MQ=255) gCCCGTTCATCGGCCACTGCCTGCCGGGCGCGGTGCGTTATACCTTGGTCGATGGCCACATCTGCCACGAGGCCTGAGTACCATGCTTAGCGGGCACGCCCGCTCCCACAGGATCACCACAATTCCAAGGCA‑TGTGGTAACCCTGTggg > 7:340203/1‑149 (MQ=255) cccGTTCATCGGCCACTGCCTGCCGGGCGCGGTGCGTTATACCTTGGTCGATGGCCACATCTGCCACGAGGCCTGAGTACCATGCTTAGCGGGCATGCCCGCTCCCACAGGGTTACCAC‑ATGCCTTGGAATTGTGGTGATCCTGTGGGa < 1:293240/149‑1 (MQ=21) cccGTTCATCGGCCACTGCCTGCCGGGCGCGGTGCGTTATACCTTGGTCGATGGCCACATCTGCCACGAGGCCTGAGTACCATGCTTAGCGGGCATGCCCGCTCCCACAGGGTTACCAC‑ATGCCTTGGAATTGTGGTGATCCTGTGGGa < 8:36928/149‑1 (MQ=21) cccGTTCATCGGCCACTGCCTGCCGGGCGCGGTGCGTTATACCTTGGTCGATGCCCACATCTGCCACGAGGCCTGAGTACCATGCTTAGCGGGCATGCCCGCTCCCACAGGGTTACCAC‑ATGCCTTGGAATTGTGGTGATCCTGTGGGa < 1:297356/149‑1 (MQ=21) ccGTTCATCGGCCACTGCCTGCCGGGCGCGGTGCGTTATACCTTGGTCGATGGCCACATCTGCCACGAGGCCTGAGTACCATGCTTAGCGGGCATGCCCGCTCCCACAGGGTTACCAC‑ATGCCTTGGAATTGTGGTGATCCTGTGGGAg < 1:292988/149‑1 (MQ=21) tCATCGGCCACTGCCTGCCGGGCGCGGTGCGTTATACCTTGGTCGATGGCCACATCTGCCACGAGGCCTGAGTACCATGCTTAGCGGGCACGCCCGCTCCCACAGGATCACCACAATTCCAAGGCA‑TGTGGTAACCCTGTGGGAGCggg > 8:156949/1‑149 (MQ=255) gCCACTGCCTGCCGGGCGCGGTGCGTTATACCTTGGTCGATGGCCACATCTGCCACGAGGCCTGAGTACCATGCTTAGCGGGCACGCCCGCTCCCACAGGATCACCACAATTCCAAGGCA‑TGTGGTAACCCTGTGGGAGCGGGCATGcc > 2:17954/1‑149 (MQ=255) ccACTGCCTGCCTTGCGCGGTGAGTTATACCTTGGTCGATGGCCACATCTGCCACGAGGCCTGAGTACCATGCTTAGCGGGCACGCCCGCTCCCACAGGATCACCACAATTCCAAGGCA‑TGTGGTAACCCTGTGGGAGCGGGCATGccc < 6:354693/149‑1 (MQ=255) cctgccGGGCGCGGTGCGTTATACCTTGGTCGATGGCCACATCTGCCACGAGGCCTGAGTACCATGCTTAGCGGGCACGCCCGCTCCCACAGGATCACCACAATTCCAAGGCA‑TGTGGTAACCCTGTGGGAGCGGGCATGCCCGCGAAt < 7:156949/149‑1 (MQ=255) tgccGGGCGCGGTGCGTTATACCTAGGTCGATGGCCACATCTGCCACCAGGCCTGAGTACCATGCTTAGCGGGCACGCACGCTCCCACACGAACACCACAAATCCACGGCA‑TGTTGTACCCCTGTGGGAGCGGGCATGCCCCCGAAAGc > 8:171130/1‑149 (MQ=255) cGGGCGCGGTGCGTTATACCTTGGTCGATGGCCACATCTGCCACGAGGCCTGAGTACCATGCTTAGCGGGCACGCCCGCTCCCACAGGATCACCACAATTCCAAGGCA‑TGTGGTAACCCTGTGGGAGCGGGCATGCCCGCGAATGCCtt > 1:105528/1‑149 (MQ=255) gcgcGGTGCGTTATACCTTGGTCGATGGCCACATCTGCCACGAGGCCTGAGTACCATGCTTAGCGGGCACGCCCGCTCCCACAGGATCACCACAATTCCAAGGCA‑TGTGGTAACCCTGTGGGAGCGGGCATGCCCGCGAATGCCTTcac < 8:340203/149‑1 (MQ=255) ggCCACATCTGCCACGAGGCCTGAGTACCATGCTTAGCGGGCACGCCCGCTCCCACAGGATCACCACAATTCCAAGGCA‑TGTGGTAACCCTGTGGGAGCGGGCATGCCCGCGAATGCCTTCACATAGTCCCCCCGCCAAGCGCGACCAt > 1:237204/1‑149 (MQ=255) ccACATCTGCCACGAGGCCTGAGTACCATGCTTAGCGGGCACGCCCGCTCCCACAGGATCACCACAATTCCAAGGCA‑TGTGGTAACCCTGTGGGAGCGGGCATGCCCGCGAATGCCTTCACATAGTCCCCCCGCCAAGCGCGACCATTc < 2:237204/149‑1 (MQ=255) caTCTGCCACGAGGCCTGAGTACCATGCTTAGCGGGCACGCCCGCTCCCACAGGATCACCACAATTCCAAGGCA‑TGTGGTAACCCTGTGGGAGCGGGCATGCCCGCGAATGCCTTCACATAGTCCCCCCGCCAAGCGCGACCATTCATc < 3:69260/149‑1 (MQ=255) aGGCCTGAGTACCATGCTTAGCGGGCACGCCCGCTCCAACAGGATCACCACAATTCCAAGGCA‑TGTGGTAACCCTGTGGGAGCGGGCATGCCCGCGAATGCCTTCACATAGTCCCCCCGCCAAGCGCGACCATTCATCCCCCCCGGTTg < 5:4801/149‑1 (MQ=255) gTACCTTGCTTAGGGGCCTCGGGCTCTGCCACAGGACCACCACATTTGCAACGCA‑TCTGGTAACCCTGGGGTAGCGGGCATGCCCGCGAATGCCTTCACATAGTCCCCCCGCCAAGCGCGACCATTCATCCCCCCCGGTTGAAATCCtt < 3:144030/149‑1 (MQ=255) tACCATGCTTAGCGGGCACGCCCGCTCCCACAGGATCACCACAATTCCAAGGCA‑TGTGGTAACCCTGTGGGAGCGGGCATGCCCGCGAATGCCTTCACATAGTCCCCCCGCCAAGCGCGACCATTCATCCCCCCCGGTTGAAATCCtt < 2:105528/148‑1 (MQ=255) cGGGAAGGCCCGCTCCAACAGTAGCACAACAATTCCAAGTCC‑TGTGGTAACCCTGTGGGAGCGGGCATGCCCGCGAATGCCTTCACATAGTCCCCCCGCCAAGCGCGACCATTCATCCCCCCCGGTTGAAATCCTTCcccccaccccc < 1:301778/148‑1 (MQ=255) gggCACGCCCGCTCCCACACGATCACCACAATTCGCAGGCA‑TGTGGATACCCAGTGGGAGCGGGCATGCCCGCGAATGCCTTCACATAGTCCCCCCTCCCAGCGCGACCATTCATCGCCCCCGGTTGAAATCCTTCCCCCCACCCCCat < 8:92870/149‑1 (MQ=255) gggCACGCCAGCTCCCACAGGATCACCACAATTCCAAGGCA‑TGTGGTAACCCTGTGGGAGCGGGCATGCCCGCGAATGCCTTCACATAGTCCCCCCGCCAAGCGCGACCATTCATCCCCCCCGGTTGAAATCCTTCCCCCCACCCCCat < 7:171130/149‑1 (MQ=255) gCACGCCCGCTCCCACAGGATCACCACAATTCCAAGGCA‑TGTGGTAACCCTGTGGGAGCGGGCATGCCCGCGAATGCCTTCACATAGTCCCCCCGCCAAGCGCGACCATTCATCCCCCCCGGTTGAAATCCTTCCCCCCACCCCCatat < 5:13655/149‑1 (MQ=255) gCACGCCCGCTCCCACAGGATCACCACAATTCCAAGGCA‑TGTGGTAACCCTGTGGGAGCGGGCATGCCCGCGAATGCCTTCACATAGTCCCCCCGCCAAGCGCGACCATTCATCCCCCCCGGTTGAAATCCTTCCCCCCACCCCCatat < 3:278291/149‑1 (MQ=255) cctcTCCCAGAGGATCCCCACATATGCAAGGCT‑TGTTGTTACCCTGTGGGAGCGGGCATGCCCGCGAATGCCTTCACATAGTCCCCCCGCCAAGCGCGACCATTCATCCCCCCCGGTTGAAATCCTTCCCCCCACCCCCATATGAGTCt < 3:313495/146‑1 (MQ=255) | CTGGCGAACAATGGTTCTCCCGTGGCGAGAACTGCCCGTTCATCGGCCACTGCCTGCCGGGCGCGGTGCGTTATACCTTGGTCGATGGCCACATCTGCCACGAGGCCTGAGTACCATGCTTAGCGGGCACGCCCGCTCCCACAGGATCACCACAATTCCAAGGCA‑TGTGGTAACCCTGTGGGAGCGGGCATGCCCGCGAATGCCTTCACATAGTCCCCCCGCCAAGCGCGACCATTCATCCCCCCCGGTTGAAATCCTTCCCCCCACCCCCATATGAGTCT > NC_002947/5696439‑5696719 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |