Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,737,793 C→T 12.2% A143T (GCC→ACC)  PP_5034 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,737,7930CT12.2% 119.9 / 7.3 49A143T (GCC→ACC) PP_5034hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (22/21);  new base T (3/3);  total (25/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.13e-01

CAGCGCCCATCAAGTTCGATCAGGCAGCACCGCCCCTGCACAACCAGCCCCAGCAATTCATCATTGCCTTCCAGCCGCAAGCAGTCGAACAACCCCAACCGCTGCGTCTCGCGCCCGGAAATAGTGACTTCGACCAGGCTGCTGGCAGCAAACAACAACACCGTCGACGCCGAGCTGTACACCCGGCTGGTGCCATCCAGCCACTGCAGCCGCGCCCGATATCGCTGCGGTGCATAGATCAGGTTGAAATCGCGGATCGCCCCGCCCAGCAACTTGCAGCTGACCTGGC  >  NC_002947/5737648‑5737936
                                                                                                                                                 |                                                                                                                                               
cAGCGCCCATCAAGTTCGATCAGGCAGCACCGCCCCTGCACAACCAGCCCCAGCAATTCATCATTGCCTTCCAGCCGCAAGCAGTCGAACAACCCCAACCGCTGCGTCTCGCGCCCGGAAATAGTGACTTCGACCAGGCTGCTGgca                                                                                                                                                <  1:106373/147‑1 (MQ=255)
  gcgcCCATCAAGTTCGATCAGGCAGCACCGCCCCTGCACAACCAGCCCCAGCAATTCATCATTGCCTTCCAGCCGCAAGCAGTCGAACAACCCCAACCGCTGCGTCTCGCGCCCGGAAATAGTGACTTCGACCAGGCTGCTGgtagca                                                                                                                                             >  8:189884/1‑148 (MQ=255)
  gcgcCCATCAAGTTCGATCAGGCAGCACCGCCCCTGCACAACCAGCCCCAGCAATTCATCATTGCCTTCCAGCCGCAAGCAGTCGAACAACCCCAACCGCTGCGTCTCGCGCCCGGAAATAGTGACTTCGACCAGGCTGCTGgcacca                                                                                                                                             >  4:256411/1‑148 (MQ=255)
    gcCCATCAAGTTCGATCAGGCAGCACCGCCCCTGCACAACCAGCCCCAGCAATTCATCATTGCCTTCCAGCCGCAAGCAGTCGAACAACCCCAACCGCTGCGTCTCGCGCCCGGAAATAGTGACTTCGACCAGGCTGCTGGCAGCAaac                                                                                                                                          <  4:84661/149‑1 (MQ=255)
     cccATCAAGTTCGATCAGGCAGCACCGCCCCTGCACAACCAGCCCCAGCAATTCATCATTGCCTTCCAGCCGCAAGCAGTCGAACAACCCCAACCGCTGCGTCTCGCGCCCGGAAATAGTGACTTCGACCAGGCTGCTGGTAGCAaaca                                                                                                                                         <  7:189884/149‑1 (MQ=255)
      ccATCAAGTTCGATCAGGCAGCACCGCCCCTGCACAACCAGCCCCAGCAATTCATCATTGCCTTCCAGCCGCAAGCAGTCGAACAACCCCAACCGCTGCGTCTCGCGCCCGGAAATAGTGACTTCGACCAGGCTGCTGGCAGCAaacaa                                                                                                                                        <  1:166345/149‑1 (MQ=255)
                 gATCAGGCAGCACCGCCCCTGCACAACCAGCCCCAGCAATTCATCATTGCCTTCCAGCCGCAAGCAGTCGAACAACCCCAACCGCTGCGTCTCGCGCCCGGAAATAGTGACTTCGACCAGGCTGCTGGCAGCAAACAACAACACCGTc                                                                                                                              >  4:277729/1‑148 (MQ=255)
                   tCAGGCAGCACCGCCCCTGCACAACCAGCCCCAGCAATTCATCATTGCCTTCCAGCCGCAAGCAGTCGAACAACCCCAACCGCTGCGTCTCGCGCCCGGAAATAGTGACTTCGACCAGGCTGCTGGCAGCAAACAACAACACCGTCGAc                                                                                                                           >  2:8283/1‑149 (MQ=255)
                    cttgcagctCCGACTCTTCACAACCAGCCCCAGCAATTCTTCATTGCCTTCCAGACGCAATCAGTCGAACAACCCCAACCGCTGCGTCTCGAGCCCGGAAATAGTGACTTCGACCAGGCTGCTGGCAGCAAACAACAACACCGTCGAc                                                                                                                           <  4:286984/145‑1 (MQ=255)
                       gcagcaCCGCCCCTGCACAACCAGCCCCAGCAATTCATCATTGCCTTCCAGCCGCAAGCAGTCGAACAACCCCAACCGCTGCGTCTCGCGCCCGGAAATAGTGACTTCGACCAGGCTGCTGGCAGCaaa                                                                                                                                           >  8:236597/1‑129 (MQ=255)
                       gcagcaCCGCCCCTGCACAACCAGCCCCAGCAATTCATCATTGCCTTCCAGCCGCAAGCAGTCGAACAACCCCAACCGCTGCGTCTCGCGCCCGGAAATAGTGACTTCGACCAGGCTGCTGGCAGCaaa                                                                                                                                           <  7:236597/129‑1 (MQ=255)
                       gcagcaCCGCCCCTGCACAACCAGCCCCAGCAATTCATCATTGCCTTCCAGCCGCAAGCAGTCGAACAACCCCAACCGCTGCGTCTCGCGCCCGGAAATAGTGACTTCGACCAGGCTGCTGGCAGCAAACAACAACACCGTCGAc                                                                                                                           >  3:286984/1‑145 (MQ=255)
                         agcaCCGCCCCTGCACAACCAGCCCCAGCAATTCATCATTGCCTTCCAGCCGCAAGCAGTCGAACAACCCCAACCGCTGCGTCTCGCGCCCGGAAATAGTGACTTCGACCAGGCTGCTGGCAGCAAACAACAACACCGTCGACGCCGAg                                                                                                                     >  2:280139/1‑149 (MQ=255)
                           caCCGCCCCTGCACAACCAGCCCCAGCAATTCATCATTGCCTTCCAGCCGCAAGCAGTCGAACAACCCCAACCGCTGCGTCTCGCGCCCGGAAATAGTGACTTCGACCAGGCTGCTGGTAGCAAACAACAACACCGTCGACGCCGAGCt                                                                                                                   <  7:277147/149‑1 (MQ=255)
                                       acaACCAGCCCCAGCAATTCATCATTGCCTTCCAGCCGCAAGCAGTCGAACAACCCCAACCGCTGCGTCTCGCGCCCGGAAATAGTGACTTCGACCAGGCTGCTGGTAGCAAACAACAACACCGTCGACGCCGAGCTGt                                                                                                                 >  4:17670/1‑139 (MQ=255)
                                       acaACCAGCCCCAGCAATTCATCATTGCCTTCCAGCCGCAAGCAGTCGAACAACCCCAACCGCTGCGTCTCGCGCCCGGAAATAGTGACTTCGACCAGGCTGCTGGTAGCAAACAACAACACCGTCGACGCCGAGCTGt                                                                                                                 <  3:17670/139‑1 (MQ=255)
                                       acaACCAGCCCCAGCAATTCATCATTGCCTTCCAGCCGCAAGCAGTCGAACAACCCCAACCGCTGCGTCTCGCGCCCGGAAATAGTGACTTCGACCAGGCTGCTGGCAGCAAACAACAACACCGTCGACGCCGAGCTGTACACCCGGCt                                                                                                       >  1:204639/1‑149 (MQ=255)
                                        caACCAGCCCCAGCAATTCATCATTGCCTTCCAGCCGCAAGCAGTCGAACAACCCCAACCGCTGCGTCTCGCGCCCGGAAATAGTGACTTCGACCAGGCTGCTGGCAGCAAACAACAACACCGTCGACGCCGAGCTGTACACCCGGCTg                                                                                                      <  3:256411/149‑1 (MQ=255)
                                                  cAGCAATTCATCATTGCCTTCCAGCCGCAAGCAGTCGAACAACCCCAACCGCTGCGTCTCGCGCCCGGAAATAGTGACTTCGACCAGGCTGCTGGCAGCAAACAACAACACCGTCGACGCCGAGCTGTACACCCGGCTGGTGCCATCCa                                                                                            <  2:204639/149‑1 (MQ=255)
                                                           atcatTGCCTTCCAGCCGCAAGCAGTCGAACAACCCCAACCGCTGCGTCTCGCGCCCGGAAATAGTGACTTCGACCAGGCTGCTGGCAGCAAACAACAACACCGTCGACGCCGAGCTGTACACCCGGCTGGTGCCATCCAGCCACTGCa                                                                                   <  1:8283/149‑1 (MQ=255)
                                                            tcatTGCCTTCCAGCCGCAAGCAGTCGAACAACCCCAACCGCTGCGTCTCGCGCCCGGAAATAGTGACTTCGACCAGGCTGCTGGCAGCAAACAACAACACCGTCGACGCCGAGCTGTACACCCGGCTGGTGCCATCCAGCCa                                                                                        >  2:170227/1‑143 (MQ=255)
                                                            tcatTGCCTTCCAGCCGCAAGCAGTCGAACAACCCCAACCGCTGCGTCTCGCGCCCGGAAATAGTGACTTCGACCAGGCTGCTGGCAGCAAACAACAACACCGTCGACGCCGAGCTGTACACCCGGCTGGTGCCATCCAGCCa                                                                                        <  1:170227/143‑1 (MQ=255)
                                                            tcatTGCCTTCCAGCCGCAAGCAGTCGAACAACCCCAACCGCTGCGTCTCGCGCCCGGAAATAGTGACTTCGACCAGGCTGCTGGCAGCAAACAACAACACCGTCGACGCCGAGCTGTACACCCGGCTGGTGCCATCCAGCCACTGCAg                                                                                  >  1:178442/1‑149 (MQ=255)
                                                               ttGCCTTCCAGCCGCAAGCAGTCGAACAACCCCAACCGCTGCGTCTCGCGCCCGGAAATAGTGACTTCGACCAGGCTGCTGGCAGCAAACAACAACACCGTCGACGCCGAGCTGTACACCCGGCTGGTGTCATCCAGCCACTGCAGCcg                                                                               >  8:210567/1‑149 (MQ=255)
                                                                   cTTCCAGCCGCAAGCAGTCGAACAACCCCAACCGCTGCGTCTCGCGCCCGGAAATAGTGACTTCGACCAGGCTGCTGGCAGCAAACAACAACACCGTCGACGCCGAGCTGTACACCCGGCTGGTGCCATCCAGCCACTGCAGCCGCGcc                                                                           >  6:244758/1‑149 (MQ=255)
                                                                     tCCAGCCGCAAGCAGTCGAACAACCCCAACCGCTGCGTCTCGCGCCCGGAAATAGTGACTTCGACCAGGCTGCTGGCAGCAAACAACAACACCGTCGACGCCGAGCTGTACACCCGGCTGGTGCCATCCAGCCACTGCAGCCGCGccc                                                                          >  6:101223/1‑148 (MQ=255)
                                                                     tCCAGCCGCAAGCAGTCGAACAACCCCAACCGCTGCGTCTCGCGCCCGGAAATAGTGACTTCGACCAGGCTGCTGGCAGCAAACAACAACACCGTCGACGCCGAGCTGTACACCCGGCTGGTGCCATCCAGCCACTGCAGCCGCGccc                                                                          <  5:101223/148‑1 (MQ=255)
                                                                          ccGCAAGCAGTCGAACAACCCCAACCGCTGCGTCTCGCGCCCGGAAATAGTGACTTCGACCAGGCTGCTGGCAGCAAACAACAACACCGTCGACGCCGAGCTGTACACCCGGCTGGTGCCATCCAGCCACTGCAGCCGCGCCCGATATc                                                                    <  3:277729/149‑1 (MQ=255)
                                                                          ccGCAAGCAGTCGAACAACCCCAACCGCTGCGTCTCGCGCCCGGAAATAGTGACTTCGACCAGGCTGCTGGCAGCAAACAACAACACCGTCGACGCCGAGCTGTACACCCGGCTGGTGCCATCCAGCCACTGCAGCCGCGCCCGATATc                                                                    >  7:67958/1‑149 (MQ=255)
                                                                                  aGTCGAACAACCCCAACCGCTGCGTCTCGCGCCCGGAAATAGTGACTTCGACCAGGCTGCTGGCAGCAAACAACAACACCGTCGACGCCGAGCTGTACACCCGGCTGGTGCCATCCAGCCACTGCAGCCGCGCCCGATATCGCTGCGGt                                                            <  8:67958/149‑1 (MQ=255)
                                                                                    tCGAACAACCCCAACCGCTGCGTCTCGCGCCCGGAAATAGTGACTTCGACCAGGCTGCTGGCAGCAAACAACAACACCGTCGACGCCGAGCTGTACACCCGGCTGGTGCCATCCAGCCACTGCAGCCGCGCCCGat                                                                       >  2:79343/1‑136 (MQ=255)
                                                                                    tCGAACAACCCCAACCGCTGCGTCTCGCGCCCGGAAATAGTGACTTCGACCAGGCTGCTGGCAGCAAACAACAACACCGTCGACGCCGAGCTGTACACCCGGCTGGTGCCATCCAGCCACTGCAGCCGCGCCCGat                                                                       <  1:79343/136‑1 (MQ=255)
                                                                                    tCGAACAACCCCAACCGCTGCGTCTCGCGCCCGGAAATAGTGACTTCGACCAGGCTGCTGGCAGCAAACAACAACACCGTCGACGCCGAGCTGTACACCCGGCTGGTGCCATCCAGCCACTGCAGCCGCGCCCGATATCGCTGCGGTGc                                                          <  1:280139/149‑1 (MQ=255)
                                                                                        acaacCCCAACCGCTGCGTCTCGCGCCCGGAAATAGTGACTTCGACCAGGCTGCTGGCAGCAAACAACAACACCGTCGACGCCGAGCTGTACACCCGGCTGGTGCCATCCAGCCACTGCAGCCGCGCCCGATATCGCTGCGGTGCa                                                         >  1:191539/1‑146 (MQ=255)
                                                                                             cccAACCGCTGCGTCTCGCGCCCGGAAATAGTGACTTCGACCAGGCTGCTGGCAGCAAACAACAACACCGTCGACGCCGAGCTGTACACCCGGCTGGTGCCATCCAGCCACTGCAGCCGCGCCCGATATCGCTGCGGTGCATAGATCAg                                                 >  2:58888/1‑149 (MQ=255)
                                                                                               cAACCGCTGCGTCTCGCGCCCGGAAATAGTGACTTCGACCAGGCTGCTGGCAGCAAACAACAACACCGTCGACGCCGAGCTGTACACCCGGCTGGTGCCATCCAGCCACTGCAGCCGCGCCCGATATCGCTGCGGTGCATAGATCAGGt                                               >  1:159535/1‑149 (MQ=255)
                                                                                                aaCCGCTGCGTCTCGCGCCCGGAAATAGTGACTTCGACCAGGCTGCTGGCAGCAAACAACAACACCGTCGACGCCGAGCTGTACACCCGGCTGGTGCCATCCAGCCACTGCAGCCGCGCCCGATATCGCTGCGGTGCATAGATCAGGtt                                              <  4:258895/149‑1 (MQ=255)
                                                                                                        cGTCTCGCGCCCGGAAATAGTGACTTCGACCAGGCTGCTGGCAGCAAACAACAACACCGTCGACGCCGAGCTGTACACCCGGCTGGTGCCATCCAGCCACTGCAGCCGCGCCCGATATCGCTGCGGTGCATAGATCAGGTTGAAATcgc                                      <  1:183835/149‑1 (MQ=255)
                                                                                                          tctcGCGCCCGGAAATAGTGACTTCGACCAGGCTGCTGGTAGCAAACAACAACACCGTCGACGCCGAGCTGTACACCCGGCTGGTGCCATCCAGCCACTGCAGCCGCGCCCGATATCGCTGCGGTGCATAGATCAGGTTGAAATcgcg                                     >  2:42238/1‑148 (MQ=255)
                                                                                                              gcgcCCGGAAATAGTGACTTCGACCAGGCTGCTGGCAGCAAACAACAACACCGTCGACGCCGAGCTGTACACCCGGCTGGTGTCATCCAGCCACTGCAGCCGCGCCCGATATCGCTGCGGTGCATAGATCAGGTTGAAATCGCGGa                                   <  7:210567/146‑1 (MQ=255)
                                                                                                                gcCCGGAAATAGTGACTTCGACCAGGCTGCTGGTAGCAAACAACAACACCGTCGACGCCGAGCTGTACACCCGGCTGGTGCCATCCAGCCACTGCAGCCGCGCCCGATATCGCTGCGGTGCATAGATCAGGTTGAAATCGCGGATcgcc                              >  8:54067/1‑149 (MQ=255)
                                                                                                                     gAAATAGTGACTTCGACCAGGCTGCTGTCATTAAAAAACTATACGGTCTACTCCGAGATGTACACCCGGCTGGTGCCATCCAGCCACTGCAGCCGCGCCCGATATCGCTGCGGTGCATAGATCAGGTTGAAATCGCGGATcgccccgcc                         <  2:191539/149‑1 (MQ=255)
                                                                                                                        aTAGTGACTTCGACCAGGCTGCTGGCAGCAAACAACAACACCGTCGACGCCGAGCTGTACACCCGGCTGGTGCCATCCAGCCACTGCAGCCGCGCCCGATATCGCTGCGGTGCATAGATCAGGTTGAAATCGCGGATCGCCCCGCCCAg                      <  1:58888/149‑1 (MQ=255)
                                                                                                                           gTGACTTCGACCAGGCTGCTGGCAGCAAACAACAACACCGTCGACGCCGAGCTGTACACCCGGCTGGTGCCATCCAGCCACTGCAGCCGCGCCCGATATCGCTGCGGTGCATAGATCAGGTTGAAATCGCGGATCGCCCCGCCCAGCaa                   >  2:355593/1‑149 (MQ=255)
                                                                                                                            tGACTTCGACCAGGCTGCTGGCAGCAAACAACAACACCGTCGACGCCGAGCTGTACACCCGGCTGGTGCCATCCAGCCACTGCAGCCGCGCCCGATATCGCTGCGGTGCATAGATCAGGTTGAAATCGCGGATCGCCCCGCCCAGCAAc                  >  1:101470/1‑149 (MQ=255)
                                                                                                                                ttCGACCAGGCTGCTGGCAGCAAACAACAACACCGTCGACGCCGAGCTGTACACCCGGCTGGTGCCATCCAGCCACTGCAGCCGCGCCCGATATCGCTGCGGTGCATAGATCAGGTTGAAATCGCGGATCGCCCCGCCCAGCAACTTg               >  6:167376/1‑148 (MQ=255)
                                                                                                                                  cGACCAGGCTGCTGGCAGCAAACAACAACACCGTCGACGCCGAGCTGTACACCCGGCTGGTGCCATCCAGCCACTGCAGCCGCGCCCGATATCGCTGCGGTGCATAGATCAGGTTGAAATCGCGGATCGCCCCGCCCAGCAACTTGCa             <  5:244758/148‑1 (MQ=255)
                                                                                                                                    aCCAGGCTGCTGGCAGCAAACAACAACACCGTCGACGCCGAGCTGTACACCCGGCTGGTGCCATCCAGCCACTGCAGCCGCGCCCGATATCGCTGCGGTGCATAGATCAGGTTGAAATCGCGGATCGCCCCGCCCAGCAACTTGCAg            >  3:305080/1‑147 (MQ=255)
                                                                                                                                    aCCAGGCTGCTGGCAGCAAACAACAACACCGTCGACGCCGAGCTGTACACCCGGCTGGTGCCATCCAGCCACTGCAGCCGCGCCCGATATCGCTGCGGTGCATAGATCAGGTTGAAATCGCGGATCGCCCCGCCCAGCAACTTGCAg            <  4:305080/147‑1 (MQ=255)
                                                                                                                                        ggCTGCTGGCAGCAAACAACAACACCGTCGACGCCGAGCTGTACACCCGGCTGGTGCCATCCAGCCACTGCAGCCGCGCCCGATATCGCTGCGGt                                                            <  6:358668/95‑1 (MQ=255)
                                                                                                                                        ggCTGCTGGCAGCAAACAACAACACCGTCGACGCCGAGCTGTACACCCGGCTGGTGCCATCCAGCCACTGCAGCCGCGCCCGATATCGCTGCGGt                                                            >  5:358668/1‑95 (MQ=255)
                                                                                                                                            gctgGCAGCAAACAACAACACCGTCGACGCCGAGCTGTACACCCGGCTGGTGCCATCCAGCCACTGCAGCCGCGCCCGAAATCGCTGCGGTGCATAGATCAGGTTGAAATCGCGGATCGCCCCGCCCAGCAACTTGCAGCTGACCTGGc  >  6:297227/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                               
CAGCGCCCATCAAGTTCGATCAGGCAGCACCGCCCCTGCACAACCAGCCCCAGCAATTCATCATTGCCTTCCAGCCGCAAGCAGTCGAACAACCCCAACCGCTGCGTCTCGCGCCCGGAAATAGTGACTTCGACCAGGCTGCTGGCAGCAAACAACAACACCGTCGACGCCGAGCTGTACACCCGGCTGGTGCCATCCAGCCACTGCAGCCGCGCCCGATATCGCTGCGGTGCATAGATCAGGTTGAAATCGCGGATCGCCCCGCCCAGCAACTTGCAGCTGACCTGGC  >  NC_002947/5737648‑5737936

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑