Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,743,394	T→C	8.0%	intergenic (‑227/+20)	fbp ← / ← glgP	class 1 fructose‑1,6‑bisphosphatase/glycogen phosphorylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,743,394	0	T	C	8.0%	168.8 / 4.7	65	intergenic (‑227/+20)	fbp/glgP	class 1 fructose‑1,6‑bisphosphatase/glycogen phosphorylase
Reads supporting (aligned to +/- strand):  ref base T (29/28);  new base C (3/2);  total (33/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.10e-01

TCGCCACGGCTCTCAGACGGCACAATAGGTCGGTAGTTCAGCCTTCGCCAGGCCGGTGGTCTGGTTGTGTGTCCATTCGCGGGCGCGCTCGCTCCCACAGGTACAGCGCAAGCCTTGAAGCTTGCGAGACACCTGTGGGAGCGGGCGTGCCCGCGAACGGCCTCACCTCAATCCAATGCCTTCCAGATCTCGGTGGCATATTCGCGAATGGTGCGGTCCGACGAGAACCAGCCCATCCGTGCCGTGTTCAATACCGCCTTGCGCCACCACTCCTGCGGTGTGTGCCACAGATCCTC  >  NC_002947/5743247‑5743542                                                                                                                                                    |                                                                                                                                                     tCGCCACGGCTCTCAGACGGCACAATAGGTCGGTAGTTCAGCCTTCGCCAGGCCGGTGGTCTGGTTGTGTGTCCATTCGCGGGCGCGCTCGCTCCCACAGGTACAGCGCAAGCCTTGAAGCTTGCGAGACACCTGTGGGAGCGGGCGTg                                                                                                                                                     >  7:210765/1‑149 (MQ=255)     cACGGCTCTCAGACGGCACAATAGGTCGGTAGTTCAGCCTTCGCCAGGCCGGTGGTCTGGTTGTGTGTCCATTCGCGGGCGCGCTCGCTCCCACAGGTACAGCGCAAGCCTTGAAGCTTGCGAGACACCTGTGGGAGCGGGCGTGCCcg                                                                                                                                                 <  6:151984/149‑1 (MQ=255)       cGGCTCTCAGACGGCACAATAGGGCGGTAGTGCAGCCTTCGCCAGGCCGGTGGTCTGGTTCTGTGTCCATTCGCGGGCGCGCTCGCTCCCACCGGTACAGCGCAAGCCTGGAAGCTTGCGAGACACCTGTCGGAGCGGGCACGCCcgct                                                                                                                                               <  5:106690/149‑2 (MQ=255)         gCTCTCAGACGGCACAATAGGTCGGTAGTTCAGCCTTCGCCAGGCCGGTGGTCTGGTTGTGTGTCCATTCGCGGGCGCGCGCGCTCCCACAGGTACAGCGCAAGCCTTGAAGCTTGCGAGACACCTGTGGGAGCGGGCGTGCCCGCGaa                                                                                                                                             <  5:180102/149‑1 (MQ=255)          ctctCAGACGGCAAAATAGGGCGGTTGTTCAGCCTTCGCCAGGCGGGTGGTCTGGTTGTGTGTCCATTCGCGGGCGCGCTCGCTCCCACAGTTACAGCGCAAGCTTTTAAGCTTGCTAGACACGTGTGGGTGGGGGGGGGCCCGCGAAc                                                                                                                                            <  8:121739/149‑1 (MQ=255)                      acaATAGGTCGGTAGTTCAGCCTTCGCCAGGCCGGTGGTCTGGTTGTGTGTCCATTCGCGGGCGCGCTCGCTCCCACAGGTACAGCGCAAGCCTTGAAGCTTGCGAGACACCTGTGGGAGCGGGCGTGCCCGCGAACGGcctcacctca                                                                                                                                <  8:210765/149‑1 (MQ=255)                      acaATAGGTCGGTAGTTCAGCCTTCGCCAGGCCGGTGGTCTGGTTGTGTGTCCATTCGCGGGCGCGCTCGCTCCCACAGGTACAGCGCAAGCCTTGAAGCTTGCGAGACACCTGTGGGAGCGGGCGTGCCCGCGAACGGcctcacctc                                                                                                                                 >  4:81152/1‑148 (MQ=255)                      acaATAGGTCGGTAGTTCAGCCTTCGCCAGGCCGGTGGTCTGGTTGTGTGTCCATTCGCGGGCGCGCTCGCTCCCACAGGTACAGCGCAAGCCTTGAAGCTTGCGAGACACCTGTGGGAGCGGGCGTGCCCGCGAACGGcctcacct                                                                                                                                  <  4:88879/147‑1 (MQ=255)                      acaATAGGTCGGTAGTTCAGCCTTCGCCAGGCCGGTGGTCTGGTTGTGTGTCCATTCGCGGGCGCGCTCGCTCCCACAGGTACAGCGCAAGCCTTGAAGCTTGCGAGACACCTGTGGGAGCGGGCGTGCCCGCGAACGGcctcacct                                                                                                                                  >  3:88879/1‑147 (MQ=255)                       caATAGGTCGGTAGTTCAGCCTTCGCCAGGCCGGTGGTCTGGTTGTGTGTCCATTCGCGGGCGCGCTCGCTCCCACAGGTACAGCGCAAGCCTTGAAGCTTGCGAGACACCTGTGGGAGCGGGCGTGCCCGCGAACGGCCTCACCTCaa                                                                                                                               <  3:81152/149‑1 (MQ=255)                         aTAGGTCGGTAGTTCAGCCTTCGCCAGGCCGGTGGTCTGGTTGTGTGTCCATTCGCGGGCGCGCTCGCTCCCACAGGTACAGCGCAAGCCTTGAAGCTTGCGAGACACCTGTGGGAGCGGGCGTGCCCGCGAACGGCCTCACCTCAATc                                                                                                                             <  4:70013/149‑1 (MQ=255)                                 gTAGTTCAGCCTTCGCCAGGCCGGTGGTCTGGTTGTGTGTCCATTCGCGGGCGCGCTCGCTCCCACAGGTACAGCGCAAGCCTTGAAGCTTGCGAGACACCTGTGGGAGCGGGCGTGCCCGCGAACGGCCTCACCTCAATCCAATGCCt                                                                                                                     >  3:343136/1‑149 (MQ=255)                                     ttCAGCCTTCGCCAGGCCGGTGGTCTGGTTGTGTGTCCATTCGCGGGCGCGGTCGCTCCCACAGGAACACCGAAAGCCTTGACGCTTGCTAGACACCTGTGGGAGCGGGCGGGCCCGCGAACGGCCTCACCTCAATCCAATGCCTTCCa                                                                                                                 <  5:213610/149‑1 (MQ=255)                                     ttCAGCCTTCGCCAGGCCGGTGGTCTGGTTGTGTGTCCATTCGCGGGCGCGCTCGCTCCCACAGGTACAGCGCAAGCCTTGAAGCTTGCGAGACACCTGTGGGAGCGGGCGTGCCCGCGAACGGCCTCACCTCAATCCAATGCCTTCCa                                                                                                                 <  5:155940/149‑1 (MQ=255)                                      tCAGCCTTCGCCAGGCCGGTGGTCTGGTTGTGTGTCCATTCGCGGGCGCGCTCGCTCCCACAGGTACAGCGCAAGCCTTGAAGCTTGCGAGACACCTGTGGGAGCGGGCGTGCCCGCGAACGGCCTCACCTCAATCCAATGCCTTc                                                                                                                   >  8:69072/1‑146 (MQ=255)                                       cAGCCTTCGCCAGGCCGGTGGTCTGGTTGTGTGTCCATTCGCGGGCGCGCTCGCTCCCACAGGTACAGCGCAAGCCTTGAAGCTTGCGAGCCACCTGTGGGAGCGGGCGTGCCCGCGAACGGCCTCACCTCAATCCAATGCCTTc                                                                                                                   >  3:82542/1‑145 (MQ=255)                                           cTTCGCCAGGCCGGTGGTCTGGTTGTGTGTCCATTCGCGGGCGCGCTCGCTCCCACAGGTACAGCGCAAGCCTTGAAGCTTGCGAGACACCTGTGGGAGCGGGCGTGCCCGCGAACGGCCTCACCTCAATCCAATGCCt                                                                                                                     >  2:283287/1‑139 (MQ=255)                                           cTTCGCCAGGCCGGTGGTCTGGTTGTGTGTCCATTCGCGGGCGCGCTCGCTCCCACAGGTACAGCGCAAGCCTTGAAGCTTGCGAGACACCTGTGGGAGCGGGCGTGCCCGCGAACGGCCTCACCTCAATCCAATGCCt                                                                                                                     <  1:283287/139‑1 (MQ=255)                                                  aGGCCGGTGGTCTGGTTGTGTGTCCATTCGCGGGCGCGCTCGCTCCCACAGGTACAGCGCAAGCCTTGAAGCTTGCGAGACACCTGTGGGAGCGGGCGTGCCCGCGAACGGCCTCACCTCAATCCAATGCCTTCCAGATCTCGGTGGCa                                                                                                    >  4:239463/1‑149 (MQ=255)                                                         tggtGTGGTTGTGTGGCATTTGGGGGGCGGGCTCTCTCCCAGAGGTACCGCGCATGCCTTGATGCTTGGGAGTCACCTGTGGGAGCGGGCGTGCCCGCGAACGGCCTCACCTCAATCCAATGCCTTCCAGATCTCGGTGGCATATTcgc                                                                                             <  1:265151/149‑1 (MQ=255)                                                                 ttGTGTGTCCATTCGCGGGCGCGCTCGCTCCCACAGGTACAGCGCAAGCCTTGAAGCTTGCGAGACACCTGTGGGAGCGGGCGTGCCCGCGAACGGCCTCACCTCAATCCAATGCCTTCCAGATCTCGGTGGCATATTCGCGAATGGTg                                                                                     <  7:69072/149‑1 (MQ=255)                                                                      tgtCCATTCGCGGGCGCGCTCGCTCCCACAGGTACAGCGCAAGCCTTGAAGCTTGCGAGACACCTGTGGGAGCGGGCGt                                                                                                                                                      >  8:110900/1‑79 (MQ=255)                                                                       gtCCATTCGCGGGCGCGCTCGCTCCCACAGGTACAGCGCAAGCCTTGAAGCTTGCGAGACACCTGTGGGAGCGGGCGTGCCCGCGAACGGCCTCCCCTCAAGCCAATGCCTTCCAGATCTCGGTGGCATATTCGCGAATGGTGCGGTcc                                                                               >  5:23851/1‑149 (MQ=255)                                                                          cATTCGCGGGCGCGCTCGCTCCCACAGGTACAGCGCAAGCCTTGAAGCTTGCGAGACACCTGTGGGAGCGGGCGTGCCCGCGAACGGCCTCACCTCAATCCAATGCCTTCCAGATCTCGGTGGCATATTCGCGAATGGTGCGGTCcga                                                                             <  7:114609/148‑1 (MQ=255)                                                                               gcgGGCGCGCTCGCTCCCACAGGTACAGCGCAAGCCTTGAAGCTTGCGAGACACCTGTGGGAGCGGGCGTGCCCGCGAACGGCCTCACCTCAATCCAATGCCTTCCAGATCTCGGTGGCATATTCGCGAATGGTg                                                                                     >  8:179484/1‑135 (MQ=255)                                                                               gcgGGCGCGCTCGCTCCCACAGGTACAGCGCAAGCCTTGAAGCTTGCGAGACACCTGTGGGAGCGGGCGTGCCCGCGAACGGCCTCACCTCAATCCAATGCCTTCCAGATCTCGGTGGCATATTCGCGAATGGTg                                                                                     <  7:179484/135‑1 (MQ=255)                                                                                       gctcgctcCCACAGGTACAGCGCAAGCCTTGAAGCTTGCGAGACACCTGTGGGAGCGGGCGTGCCCGCGAACGGCCTCACCTCAATCCAATGCCTTCCAGATCTCGGTGGCATATTCGCGAATGGTGCGGTCCGACGAGAACCAGCCCa                                                               >  5:10424/1‑149 (MQ=255)                                                                                       gcccgctcccacaggtgtctcgcaagcttcaaggcttgcgctgtacctgtgggagcgGGCGTGCCCGCGAACGGCCTCACCTCAATCCAAt                                                                                                                         >  8:294192‑M2/58‑91 (MQ=255)                                                                                       gcccgctcccacaggtgtctcgcaagcttcaaggcttgcgctgtacctgtgggagcgGGCGTGCCCGCGAACGGCCTCACCTCAATCCAAt                                                                                                                         <  7:294192‑M2/34‑1 (MQ=255)                                                                                          CGCTCCCACAGGTCCAGCGCAAGCCTTGACGCTTGCGTGACCCCTGTGGGAGCGGGCGGGCCCGCGAACGGCCTCACCTCAATCCAATGCCTTCCAGATCTCGGTGGCATATTCGCGAATGGTGCGGTCCGACGAGAACCAGCCCATcc                                                            >  5:195300‑M1/1‑149 (MQ=255)                                                                                           gcgcccACTGGTCCCGCGCAAGCCGGGAAGCTGGCGAGCCCCCTGTGGTCGCGGGCGTGCCCGCGAACGGCCTCACCTCAATCCAATGCCTTCCAGATCTCGGTGGCATATTCGCGAATGGTGCGGTCCGACGAGAACCAGCCCATcc                                                            <  5:190348/145‑1 (MQ=255)                                                                                           GCTCCCACAGGTACAGCGCAAGCCTTGAAGCTTGCGAGACACCTGTGGGAGCGGGCGTGCCCGAGAACGGCCTCACCTCAATCCAATGCCTTCCAGATCTCGGTGGCATATTCGCGAATGGTGCGGTCCGACGAGAACCAGCCCATccg                                                           <  1:5623‑M1/149‑1 (MQ=255)                                                                                             tcccacaggtgtctcgcaagcttcaaggcttgcgctgtacctgtgggagcgAGCGCGCCCGCGAATGGACACACCTCAATCCAATGCCTTCCAGATCTCGGTGGCATATTCGCGAATGGTGCGGTCCGACGAGAACCAGCCCATccgtg                                                         >  2:332878‑M2/52‑149 (MQ=255)                                                                                              CCCACAGGTACAGCGCAAGCCTTGAAGCTTGCGAGACACCTGTGGGAGCGGGCGTGCCCGCGAACGGCCTCACCTCAATCCAATGCCTTCCAGATCTCGGTGGCATATTCGCGAATGGTGCGGt                                                                                 <  5:306923‑M1/124‑1 (MQ=255)                                                                                              CCCACAGGTACAGCGCAAGCCTTGAAGCTTGCGAGACACCTGTGGGAGCGGGCGTGCCCGCGAACGGCCTCACCTCAATCCAATGCCTTCCAGATCTCGGTGGCATATTCGCGAATGGTGCGGt                                                                                 >  6:306923‑M1/1‑124 (MQ=255)                                                                                               ccacaggtgtctcgcaagcttcaaggcttgcgctgtacctgtgggagcgGGCGTGCCCGCGAACGGCCTCACCTCAATCCAATGCCTTCCAGATCTCGGTGGCATATTCGCGAATGGTGCGGTCCGACGAGAACCAGCCCATccgtgcc                                                       >  7:309913‑M2/50‑149 (MQ=255)                                                                                               ccacaggtgtctcgcaagcttcaaggcttgcgctgtacctgtgggagcgAGCGTGCCCGCGAACGGCCTCACCTCAATCCAATGCCTTCCAGATCTCGGTGGCATATTCGCGAATGGTGCGGTCCGACGAGAACCAGCCCATccgtgcc                                                       >  3:234445‑M2/50‑149 (MQ=255)                                                                                                CACAGGTACAGCGCAAGCCTTGAAGCTTGCGAGACACCTGTGGGAGCGGGCGTGCCCGCGAACGGCCTCACCTCAATCCAATGCCTTCCAGATCTCGGTGGCATATTCGCGAATGGTGCGGTCCGACGAGAACCAGCCCATccgtgccg                                                      <  8:299771‑M1/149‑1 (MQ=255)                                                                                                 acaggtgtctcgcaagcttcaaggcttgcgctgtacctgtgggagcgAGCGCGCCCGCGAACGGCCTCACCTCAATCCAATGCCTTCCAGATCTCGGTGGCATATTCGCGAATGGTGCGGTCCGACGAGAACCAGCCCATccgtgccgt                                                     >  1:110659‑M2/48‑149 (MQ=255)                                                                                                    GGTCCAGCGCAAGCCTTCACGCTGGCGAGACACCTGGGGGCGCGGGCGTGCCCGCGAACGGCCTCACCTCAATCCAATGCCTTCCAGATCTCGGTGGCATATTCGCGAATGGTGCGGTCCGACGAGAACCAGCCCATCCGTGCCGTGtt                                                  >  6:23851‑M1/1‑149 (MQ=255)                                                                                                     gtgtctcgcaagcttcaaggcttgcgctgtacctgtgggagcgGGCGTGCCCGCGAACGGCCTCACCTCAATCCAATGCCTTCCAGATCTCGGTGGCATATTCGCGAATGGTGCGGTCCGACGAGAACCAGCCCATCCGTGCCGTGTTc                                                 <  7:37569‑M2/106‑1 (MQ=255)                                                                                                     gtgtctcgcaagcttcaaggcttgcgctgtacctgtgggagcgAGCGCGCCCGCGAATGGCCTCACCTCAATCCAATGCCTTCCAGATCTCGGTGGCATATTCGCGAATGGTGCGGTCCGACGAGAACCAGCCCATCCGTGCCGTGtt                                                  >  6:153578‑M2/44‑148 (MQ=255)                                                                                                        tctcgcaagcttcaaggcttgcgctgtacctgtgggagcgGGCGTGCCCGCGAACGGCCTCACCTCAATCCAATGCCTTCCAGATCTCGGTGGCa                                                                                                    >  6:100099‑M2/41‑95 (MQ=255)                                                                                                        tctcgcaagcttcaaggcttgcgctgtacctgtgggagcgGGCGTGCCCGCGAACGGCCTCACCTCAATCCAATGCCTTCCAGATCTCGGTGGCa                                                                                                    <  5:100099‑M2/55‑1 (MQ=255)                                                                                                        tctcgcaagcttcaaggcttgcgctgtacctgtgggagcgAGCGCGCCCGCGAACGGCCTCACCTCAATCCAATGCCTTCCAGATCTCGGTGGCATATTCGCGAATGGTGCGGTCCGACGAGAACCAGCCCATCCGTGCCGTGTTCAAt                                              <  2:110659‑M2/109‑1 (MQ=255)                                                                                                           cgcAAGCCTTGAAGCTTGCGAGACACCTGTGGGAGCGGGCGTGCCCGCGAACGGCCTCACCTCAATCCAATGCCTTCCAGATCTCGGTGGCATATTCGCGAATGGTGCGGTCCGACGAGAACCAGCCCATCCGTGCCGTGTTCAATAcc                                           >  7:5650/1‑149 (MQ=255)                                                                                                           cgcAAGCCTTGAAGCTTGCGAGACACCTGTGGGAGCGGGCGTGCCCGCGAACGGCCTCACCTCAATCCAATGCCTTCCAGATCTCGGTGGCATATTCGCGAATGCTGCGGTCCGACGAGAACCAGCCCATCCGTGCCCTGTTCAATAcc                                           >  4:47027/1‑149 (MQ=255)                                                                                                              aaGCCTTGAAGCTTGCGAGACACCTGTGGGAGCGGGCGTGCCCGCGAACGGCCTCACCTCAATCCAATGCCTTCCAGATCTCGGTGGCATATTCGCGAATGGTGCGGTCCGACGAGAACCAGCCCATCCGTGCCGTGTTCAATACCGcc                                        >  8:252169/1‑149 (MQ=255)                                                                                                              aaGCCTTGAAGCTTGCGAGACACCTGTGGGAGCGGGCGTGCCCGCGAACGGCCTCACCTCAATCCAATGCCTTCCAGATCTCGGTGGCATATTCGCGAATGGTGCGGTCCGACGAGAAACAGCCCATCCGTGACGTGTTCAATCCAGcc                                        >  8:257093/1‑149 (MQ=255)                                                                                                                  cTTGAAGCTTGCGAGACACCTGTGGGAGCGGGCGTGCCCGCGAACGGCCTCACCTCAATCCAATGCCTTCCAGATCTCGGTGGCATATTCGCGAATGGTGCGGTCCGACGAGAACCAGCCCATCCGTGCCGTGTTCAATACCGCCTTgc                                    >  4:302326/1‑149 (MQ=255)                                                                                                                  cTTGAAGCTTGCGAGACACCTGTGGGAGCGGGCGTGCCCGCGAACGGCCTCACCTCAATCCAATGCCTTCCAGATCTCGGTGGCATATTCGCGAATGGTGCGGTCCGACGAGAACCAGCCCATCCGTGCCGTGTTCAATACCGCCTTgc                                    >  5:103286/1‑149 (MQ=255)                                                                                                                  agtGATGCTTGCGAGACACCTGTGGGAGCGGGCGTGCCCGCGAACGGCCTCACCTCAATCCAATGCCTTCCAGATCTCGGTGGCATATTCGCGAATGGTGCGGTCCGACGAGAACCAGCCCATCCGTGCCGTGTTCAATACCGCCTTgc                                    <  4:82542/147‑1 (MQ=255)                                                                                                                    tGAAGCTTGCGAGACACCTGTGGGAGCGGGCGTGCCCGCGAACGGCCTCACCTCAATCCAATGCCTTCCAGATCTCGGTGGCATATTCGCGAATGGTGCGGTCCGACGAGAACCAGCCCATCCGTGCCGTGTTCAATACCGCCTTgcgc                                  >  1:213275/1‑149 (MQ=255)                                                                                                                       aGCTTGCGAGACACCTGTGGGAGCGGGCGTGCCCGCGAACGGCCTCACCTCAATCCAATGCCTTCCAGATCTCGGTGGCATATTCGCGAATGGTGCGGTCCGACGAGAACCAGCCCATCCGTGCCGTGTTCAATACCGCCTTGCGccac                               <  7:257093/149‑1 (MQ=255)                                                                                                                       aGCTTGCGAGACACCTGTGGGAGCGGGCGTGCCCGCGAACGGCCTCACCTCAATCCAATGCCTTCCAGATCTCGGTGGCATATTCGCGAATGGTGCGGTCCGACGAGAACCAGCCCATCCGTGCCGTGTTCAATACCGCCTTGCGccac                               <  7:252169/149‑1 (MQ=255)                                                                                                                             cGAGACACCTGTGGGAGCGGGCGTGCCCGCGAACGGCCTCACCTCAATCCAATGCCTTCCAGATCTCGGTGGCATATTCGCGAATGGTGCGGTCCGACGAGAACCa                                                                    >  2:177249/1‑106 (MQ=255)                                                                                                                             cGAGACACCTGTGGGAGCGGGCGTGCCCGCGAACGGCCTCACCTCAATCCAATGCCTTCCAGATCTCGGTGGCATATTCGCGAATGGTGCGGTCCGACGAGAACCa                                                                    <  1:177249/106‑1 (MQ=255)                                                                                                                             cGAGACACCTGTGGGAGCGGGCGTGCCCGCGAACGGCCTCACCTCAATCCAATGCCTTCCAGATCTCGGTGGCATATTCGCGAATGGTGCGGTCCGACGAGAACCAGCCCATCCGTGCCGTGTTCAATACCGCCTTGCGCCACCACTcc                         <  2:213275/149‑1 (MQ=255)                                                                                                                             cGAGACACCTGTGGGAGCGGGCGTGCCCGCGAACGGCCTCACCTCAATCCAATGCCTTCCAGATCTCGGTGGCATATTCGCGAATGGTGCGGTCCGACGAGAACCAGCCCATCCGTGCCGTGTTCAATACCGCCTTGCGCCACCACTc                          <  8:5650/148‑1 (MQ=255)                                                                                                                              gagaCACCTGTGGGAGCGGGCGTGACCGCGAACGGCCTCACCTCAATCCAATGCCTTCCAGATCTCGGTGGCATAGTCGCGAATGGTGCGGTCCGACGAGAACCAGCCCATCCGTGCCGTGTTCAATACCGCCTTGCGACACCACTCCt                        >  5:348338/1‑149 (MQ=255)                                                                                                                                gaCACCTGTGGGAGCGGGCGTGCCCGCGAACGGCCTCACCTCAATCCAATGCCTTCCAGATCTCGGTGGCATATTCGCGAATGGTGCGGTCCGACGAGAACCAGCCCATCCGTGCCGTGTTCAATACCGCCTTGCGCCACCACTCCTGc                      >  8:330733/1‑149 (MQ=255)                                                                                                                                    cctgtgggagcgGGCGTGCCCGCGAACGGCCTCACCTCAATCCAAAGCCTTCCAGATCTCGGTGGCATATTCGCGAATGGTGCGGTACGACGAGAACCAGCACATCCGTGCCGTGTTCAATACCGCCTTGCGCCACCACTCCTGCGgtg                  >  4:223601‑M2/13‑149 (MQ=255)                                                                                                                                     ctgtgggagcgGGCGTGCCCGCGAACGGCCTCACCTCAATCCAATGCCTTCCAGATCTCGGTGGCATATTCGCGAATGGTGCGGTCCGACGAGAACCAGCCCATCCGTGCCGTGTTCAATACCGCCTTGCGCCACCACTCCTGCGgtgt                 <  6:10424‑M2/138‑1 (MQ=255)                                                                                                                                         gggagcgGGCGTGCCCGCGAACGGCCTCACCTCAATCCAATGCCTTCCAGATCTCGGTGGCATATTCGCGAATGGTGCGGTCCGACGAGAACCAGCCCATCCGTGCCGTGTTCAATACCGCCTTGCGCCACCACTCCTGCGGTTTGTGc             <  7:330733‑M2/142‑1 (MQ=255)                                                                                                                                           gagcgGGCGTGCCCGCGAACGGCCTCACCTCAATCCAATGCCTTCCAGATCTCGGTGGCATATTCGCGAATGGTGCGGTCCGACGAGAACCAGCCCATCCGTGCCGTGTTCAATACCGCCTTGCGCCACCACTCCTGCGGTGTGTGCca           >  3:245632‑M2/6‑149 (MQ=255)                                                                                                                                                  cGTGCCCGCGAACGGCCTCACCTCAATCCAATGCCTTCCAGATCTCGGTGGCATATTCGCGAATGGTGCGGTCCGACGAGAACCAGCCCATCCGTGCCGTGTTCAATACCGCCTTGCGCCACCACTCCTGCGGTGTGTGCCACAGATcc    <  3:239463/149‑1 (MQ=255)                                                                                                                                                    tGCCCGCGAACGGCCTCACCTCAATCCAATGCCTTCCAGATCTCGGTGGCATATTCGCGAATGGTGCGGTCCGACGAGAACCAGCCCATCCGTGCCGTGTTCAATACCGCCTTGCGCCACCACTCCTGCGGTGTGTGCCACAGATCCTc  >  4:286819/1‑149 (MQ=255)                                                                                                                                                    |                                                                                                                                                     TCGCCACGGCTCTCAGACGGCACAATAGGTCGGTAGTTCAGCCTTCGCCAGGCCGGTGGTCTGGTTGTGTGTCCATTCGCGGGCGCGCTCGCTCCCACAGGTACAGCGCAAGCCTTGAAGCTTGCGAGACACCTGTGGGAGCGGGCGTGCCCGCGAACGGCCTCACCTCAATCCAATGCCTTCCAGATCTCGGTGGCATATTCGCGAATGGTGCGGTCCGACGAGAACCAGCCCATCCGTGCCGTGTTCAATACCGCCTTGCGCCACCACTCCTGCGGTGTGTGCCACAGATCCTC  >  NC_002947/5743247‑5743542

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑