Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,762,486 T→C 9.7% intergenic (+2/‑139) gpmI → / → PP_5057 2,3‑bisphosphoglycerate‑independent phosphoglycerate mutase/M23/M37 family peptidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,762,4860TC 9.7% 108.0 / 2.8 41intergenic (+2/‑139)gpmI/PP_50572,3‑bisphosphoglycerate‑independent phosphoglycerate mutase/M23/M37 family peptidase
Reads supporting (aligned to +/- strand):  ref base T (20/17);  new base C (2/2);  total (22/19)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.19e-01

GGTACCGTTCATCTACGTGGGCAAGCGCAACGTGAAAGTGCGCGAGGGCGGCGTGCTGGCCGACGTGGCGCCAACCATGCTGAAGCTGCTGGGGCTGGAAAAGCCGGTGGAAATGACCGGTACCTCGATTCTGGTCGACGCCTGAATGTCGAAAGGGGCTGCGTTGCAG‑CCCCAATTGCACATGAATT‑‑CCAGACAAACGCCCCCCTGCATCCGCAGCGAGGCGTTTTTTTTGCCTGCCCAGGCGGGCATACTAGGCCAGTCTCCATCCTTGGTACGCCAAACCCCATG  >  NC_002947/5762340‑5762627
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ggTACCGTTCATCTACGTGGGCAAGCGCAACGTGAAAGTGCGCGAGGGCGGCGTGCTGGCCGACGTGGCGCCAACCATGCTGAAGCTGCTGGGGCTGGAAAAGCCGGTGGAAATGACCGGTACCTCGATTCTGGTCGACGCCTGAATGt                                                                                                                                                >  6:250082/1‑149 (MQ=255)
ggTACCGTTCATCTACGTGGGCAAGCGCAACGTGAAAGTGCGCGAGGGCGGCGTGCTGGCCGACGTGGCGCCAACCATGCTGAAGCTGCTGGGGCTGGAAAAGCCGGTGGAAATGACCGGTACCTCGATTCTGGTCGACGCCTGAATGt                                                                                                                                                >  1:4767/1‑149 (MQ=255)
 gTACCGTTCATCTACGTGGGCAAGCGCAACGTGAAAGTGCGCGAGGGCGGCGTGCTGGCCGACGTGGCGCCAACCATGCTGAAGCTGCTGGGGCTGGAAAAGCCTGTGGAAATGACCTGTACCTCGATTCTGGTCGACGCCTGAATGTc                                                                                                                                               <  1:74951/149‑1 (MQ=255)
             tACGTGGGCAAGCGCAACGTGAAAGTGCGCGAGGGCGGCGTGCTGGCCGACGTGGCGCCAACCATGCTGAAGCTGCTGGGGCTGGAAAAGCCGGTGGAAATGACCGGTACCTCGATTCTGGTCGACGCCTGAATGTCGAAAGGGGCTGc                                                                                                                                   >  7:3589/1‑149 (MQ=255)
               cGTGGGCAAGCGCAACGTGAAAGTGCGCGAGGGCGGCGTGCTGGCCGACGTGGCGCCAACCATGCTGAAGCTGCTGGGGCTGGAAAAGCCGGTGGAAATGACCGGTACCTCGATTCTGGTCGACGCCTGAATGTCGAAAGGGGCt                                                                                                                                     <  5:222904/145‑1 (MQ=255)
               cGTGGGCAAGCGCAACGTGAAAGTGCGCGAGGGCGGCGTGCTGGCCGACGTGGCGCCAACCATGCTGAAGCTGCTGGGGCTGGAAAAGCCGGTGGAAATGACCGGTACCTCGATTCTGGTCGACGCCTGAATGTCGAAAGGGGCTg                                                                                                                                    <  2:136726/146‑1 (MQ=255)
                gTGGGCAAGCGCAACGTGAAAGTGCGCGAGGGCGGCGTGCTGGCCGACGTGGCGCCAACCATGCTGAAGCTGCTGGGGCTGGAAAAGCCGGTGGAAATGACCGGTACCTCGATTCTGGTCGACGCCTGAATGTCGAAAGGGGCTGCGtt                                                                                                                                <  5:106341/149‑1 (MQ=255)
                 tGGGCAAGCGCAACGTGAAAGGTCTCGAGGGCGGCGTGCTGGCCGACGTGGCGCCAACCATGCTGAAGCTGCTGGGGCTGGAAAAGCCGGTGGAAATGACCGGTACCTCGATTCTGGTCGACGCCTGAATGTCGAAAGGGGCTGCGTTg                                                                                                                               <  7:337689/149‑1 (MQ=255)
                   ggCAAGCGCAACGTGAAAGTGCGCGAGGGCGGCGTGCTGGCCGACGTGGCGCCAACCATGCTGAAGCTGCTGGGGCTGGAAAAGCCGGTGGAAATGACCGGTACCTCGATTCTGGTCGACGCCTGAACGTCGAAAGGGGCTGCGTTg                                                                                                                               >  6:140600/1‑147 (MQ=255)
                    gCAAGCGCAACGTGAAAGTGCGCGAGGGCGGCGTGCTGGCCGACGTGGCGCCAACCATGCTGAAGCTGCTGGGGCTGGAAAAGCCGGTGGAAATGACCGGTACCTAGATTCTGGTCGACGCCTGAATGTCGAAAGGGGCTGCGTTGCAg                                                                                                                            >  5:8829/1‑149 (MQ=255)
                            aaCGTGAAAGTGCGCGAGGGCGGCGTGCTGGCCGACGTGGCGCCAACCATGCTGAAGCTGCTGGGGCTGGAAAAGCCGGTGGAAATGACCGGTACCTCGATTCTGGTCGACGCCTGAATGTCGAAAGGGGCTGCGTTGCAG‑CCCCAAtt                                                                                                                   >  3:115896/1‑149 (MQ=255)
                            aaCGTGAAAGTGCGCGAGGGCGGCGTGCTGGCCGACGTGGCGCCAACCATGCTGAAGCTGCTGGGGCTGGAAAAGCCGGTGGAAATGACCGGTACCTCGATTCTGGTCGACGCCTGAACGTCGAAAGGGGCTGCGTTGCAG‑CCCCAAtt                                                                                                                   <  5:140600/149‑1 (MQ=255)
                                  aaaGTGCGCGAGGGCGGCGTGCTGGCCGACGTGGCGCCAACCATGCTGAAGCTGCTGGGGCTGGAAAAGCCGGTGGAAATGACCGGTACCTCGATTCTGGTCGACGCCTGAATGTCGAAAGGGGCTGCGTTGCAG‑CCCCAATTGcaca                                                                                                              <  7:303865/148‑1 (MQ=255)
                                   aaGTGCGCGAGGGCGGCGTGCTGGCCGACGTTGCGCCAACCATGCTGAAGCTGCTGGGGCTGGAAAAGCCGGTGGAAATGACCGGTACCTCGATTCTGGTCGACGCCTGAATGTCGAAAGGGGCTGCGTTGCAG‑CCCCAATTGCACAt                                                                                                             <  4:9911/148‑1 (MQ=255)
                                   aaGTGCGCGAGGGCGGCGTGCTGGCCGACGTGGCGCCAACCATGCTGAAGCTGCTGGGGCTGGAAAAGCCGGTGGAAATGACCGGTACCTCGATTCTGGTCGACGCCTGAATGTCGAAAGGGGCTGCGTTGCAG‑CCCCAATTGCACATg                                                                                                            <  5:250082/149‑1 (MQ=255)
                                     gTGCGCGAGGGCGGCGTGCTGGCCGACGTGGCGCCAACCATGCTGAAGCTGCTGGGGCTGGAAAAGCCGGTGGAAATGACCGGTACCTCGATTCTGGTCGACGCCTGAATGTCGAAAGGGGCTGCGTTGCAG‑CCCCAATTGCACATGaa                                                                                                          <  4:115896/149‑1 (MQ=255)
                                             gggCGGCGTGCTGGCCGACGTGGCGCCAACCATGCTGAAGCTGCTGGTGCTGGAAAAGCCGGTGGAAATGACCGGTACCTCGATTCTGGTCGACGCCTGAATGTCGAAAGGGGCTGCGTTGCAG‑CCCCAATTGCACATGAATT‑‑CCAGa                                                                                                 >  2:97156/1‑148 (MQ=255)
                                               gcggcgTGCTGGCCGACGTGGCGCCAACCATGCTGAAGCTGCTGGGGCTGGAAAAGCCGGTGGAAATGACCGGTACCTCGATTCTGGTCGACGCCTGAATGTCGAAAGGGGCTGCGTTGCAG‑CCCCAATTGCACATGAATT‑‑CCAGACa                                                                                               >  2:102346/1‑148 (MQ=255)
                                                         ggCCGACGTGGCGCCATCCATGCTGAAGCTGCTGGGGCTGGAAAAGCCGGTGGAAATGACCGGTACCTCGATTCTGGTCGACGCCTGAATGTCGAAAGGGGCTGCGTTGCAG‑CCCCAAtt                                                                                                                   <  1:55006/120‑1 (MQ=255)
                                                         ggCCGACGTGGCGCCATCCATGCTGAAGCTGCTGGGGCTGGAAAAGCCGGTGGAAATGACCGGTACCTCGATTCTGGTCGACGCCTGAATGTCGAAAGGGGCTGCGTTGCAG‑CCCCAAtt                                                                                                                   >  2:55006/1‑120 (MQ=255)
                                                          gCCGACGTGGCGCCAACCATGCTGAAGCTGCTGGGGCTGGAAAAGCCGGTGGAAATGACCGGTACCTCGATTCTGGTCGACGCCTGAATGTCGAAAGGGGCTGCGTTGCAG‑CCCCAATTGCACATGAATT‑‑CCAGACAAACGCCCCCCt                                                                                    <  2:4767/148‑1 (MQ=255)
                                                                     gcCAACCATGCTGAAGCTGCTGGGGCTGGAAAAGCCGGTGGAAATGACCGGTACCTCGATTCTGGTCGACGCCTGAATGTCGAAAGGGGCTGCGTTGCAG‑CCCCAATTGCACATGAATT‑‑CCAGACAAACGCCCCCCTGCATCCGCAGc                                                                         >  4:39700/1‑148 (MQ=255)
                                                                          ccATGCTGAAGCTGCTGGGGCTGGAAAAGCCGGTGGAAATGACCGGTACCTCGATTCTGGTCGACGCCTGAATGTCGAAAGGGGCTGCGTTGCAG‑CCCCAATTGCACATGAATT‑‑CCATACAAACGCCCCCCTGCATCCGCAGCGAGGCg                                                                   >  8:211031/1‑149 (MQ=255)
                                                                             tGCTGAAGCTGCTGGGGCTGGAAAAGCCGGTGGAAATGACCGGTACCTCGATTCTGGTCGACGCCTGAATGTCGAAAGGGGCTGCGTTGCAG‑CCCCAATTGCACATGAATT‑‑CCAGACAAACGCCCCCCTGCATCCGCa                                                                           >  6:345970/1‑138 (MQ=255)
                                                                             tGCTGAAGCTGCTGGGGCTGGAAAAGCCGGTGGAAATGACCGGTACCTCGATTCTGGTCGACGCCTGAATGTCGAAAGGGGCTGCGTTGCAG‑CCCCAATTGCACATGAATT‑‑CCAGACAAACGCCCCCCTGCATCCGCa                                                                           <  5:345970/138‑1 (MQ=255)
                                                                               cTGAAGCTGCTGGGGCTGGAAAAGCCGGTGGAAATGACCGGTACCTCGATTCTGGTCGACGCCTGAACGTCGAAAGGGGCTGCGTTGCAG‑CCCCAATTGCACATGAATT‑‑CCAGACAAACGCCCCCCTGCATCCGCAGCGAGGCGttttt                                                              >  8:254804/1‑149 (MQ=255)
                                                                                 gAAGCTGCTGGGGCTGGAAAAGCCGGTGGAAATGACCGGTACCTCGATTCTGGTCGACGCCTGAATGTCGAAAGGGGCTGCGTTGCAG‑CCCCAATTGCACATGAATT‑‑CCAGACAAACGCCCACCTGCATCCGCAGCGAGGCGttttttt                                                            >  7:27196/1‑149 (MQ=255)
                                                                                  aaGCTGCTGGGGCTGGAAAAGCCGGTGGAAATGACCGGTACCTCGATTCTGGTCGACGCCTGAATGTCGAAAGGGGCTGCGTTGCAGCCCCCAATTGCACATGAATT‑‑CCAGACAAACGCCTCGCTGCGGATGCAGGGGGGCGtttgtc                                                             <  1:102346/148‑4 (MQ=38)
                                                                                              cTGGAAAAGCCGGTGGAAATGACCGGTACCTCGATTCTGGTCGACGCCTGAATGTCGAAAGGGGCTGCGTTGCAG‑CCCCAATTGCACATGAATT‑‑CCAGACAAACGCCCCCCTGCATCCGCAGCGAGGCGTTTTTTTTGCCTGCCCAGGc                                               <  1:97156/149‑1 (MQ=255)
                                                                                                 gAAAAGCCGGTGGAAATGACCGGTACCTCGATTCTGGTCGACGCCTGAATGTCGAAAGGGGCTGCGTTGCAG‑CCCCAATTGCACATGAATT‑‑CCAGACAAACGCCCCCCTGCATCCGCAGCGAGGCGTTTTTTTTGCCTGCCCAGGCggg                                            <  8:3589/149‑1 (MQ=255)
                                                                                                      gCCGGTGGAAATGACCGGTACCTCGATTCTGGTCGACGCCTGAACGTCGAAAGGGGCTGCGTTGCAG‑CCCCAATTGCACATGAATT‑‑CCAGACAAACGCCCCCCTGCATCCGCAGCGAGGCGTTTTTTTTGCCTGCCCAGGCGGGCATAc                                       <  7:254804/149‑1 (MQ=255)
                                                                                                       ccGGTGGAAATGACCGGTACCTCGATTCTGGTCGACGCCTGAATGTCGAAAGGGGCTGCGTTGCAG‑CCCCAATTGCACATGAATT‑‑CCAGACAAACGCCCCCCTGCATCCGCAGCGAGGCGTTTTTTTTGCCTGCCCAGGCGGGCATACt                                      <  3:39700/149‑1 (MQ=255)
                                                                                                        cGGTGGAAATGACCGGTACCTCGATTCTGGTCGACGCCTGAATGTCGAAAGGGGCTGCGTGGCAG‑CCCCAATTGCACATGAATT‑‑CCAGACAAACGCCCCCCTGCATCCGCAGCGAGGCGTTTTTTTTGCCTGCCCAGGCGGGCATACTa                                     <  6:267116/149‑1 (MQ=255)
                                                                                                         ggTGGAAATGACCGGTACCTCGATTCTGGTCGACGCCTGAATGTCGAAAGGGGCTGCGTTGCAG‑CCCCAATTGCACATGAATT‑‑CCAGACAAACGCCCCCCTGCATCCGCAGCGAGGCGTTTTTTTTGCCTGCCCAGGCGGGCATACTAg                                    >  1:154735/1‑149 (MQ=255)
                                                                                                                           cTCGATTCTGGTCGACGCCTGAATGTCGAAAGGGGCTGCGTTGCAG‑CCCCAATTGCACATGAATT‑‑CCAGACAAACGCCCCCCTGCATCCGCAGCGAGGCGTTTTTTTTGCCTGCCCAGGCGGGCATACTAGGCCAGTCTCCATCCTTgg                  >  3:239121/1‑149 (MQ=255)
                                                                                                                           cTCGATTCTGGTCGACGCCTGAATGTCGAAAGGGGCTGCGTTGCAG‑CCCCAATTGCACATGAATT‑‑CCAGACAAACGCCCCCCTGCATCCGCAGCGAGGCG‑TTTTTTTGCCTGCCCAGGCGGGCATACTAGGCCAGTCTCCATCCTTgg                  >  4:330941/1‑148 (MQ=255)
                                                                                                                            tCGATTCTGGTCGACGCCTGAATGTCGAAAGGGGCTGCGTTGCAG‑CCCCAATTGCACATGAATT‑‑CCAGACAAACGCCCCCCTGCATCCGCAGCGAGGCGTTTTTTTTGCCTGCCCAGGCGGGCATACTAGGCCAGTCTCCATCCTTgg                  >  6:239212/1‑148 (MQ=255)
                                                                                                                            tCGATTCTGGTCGACGCCTGAATGTCGAAAGGGGCTGCGTTGCAG‑CCCCAATTGCACATGAATT‑‑CCAGACAAACGCCCCCCTGCATCCGCAGCGAGGCGTTTTTTTTGCCTGCCCAGGCGGGCATACTAGGCCAGTCTCCATCCTTgg                  >  1:136035/1‑148 (MQ=255)
                                                                                                                            tCGATTCTGGTCGACGCCTGAATGTCGAAAGGGGCTGCGTTGCAG‑CCCCAATTGCACATGAATT‑‑CCAGACAAACGCCCCCCTGCATCCGCAGCCACGCGTTTTTTATGCCTGCCCAGGCGGGCATACTAGGCCAGACTCCATccatcg                  >  5:236703/1‑144 (MQ=255)
                                                                                                                              gATTCTGGTCGACGCCTGAATGTCGAAAGGGGCTGCGTTGCAG‑CCCCAATTGCACATGAATTGGCCAGACAAACGCCCCCCTGCATCCGCAGCGAGGCGTTTTTTTTGCCTGCCCAGGCGGGCATACTAGGCCAGTCTCCATCCTTgg                  >  1:103317/1‑148 (MQ=255)
                                                                                                                                           gCCTGAATGTCGAAAGGGGCTGCGTTGCAG‑CCCCAATTGCACATGAATT‑‑CCAGACAAACGCCCCCCTGCATCCGCAGCGAGGCGTTTTTTTTGCCTGCCCAGGCGGGCATACTAGGCCAGTCTCCATCCTTGGTACGCCAAACCCCATg  >  7:83506/1‑149 (MQ=255)
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GGTACCGTTCATCTACGTGGGCAAGCGCAACGTGAAAGTGCGCGAGGGCGGCGTGCTGGCCGACGTGGCGCCAACCATGCTGAAGCTGCTGGGGCTGGAAAAGCCGGTGGAAATGACCGGTACCTCGATTCTGGTCGACGCCTGAATGTCGAAAGGGGCTGCGTTGCAG‑CCCCAATTGCACATGAATT‑‑CCAGACAAACGCCCCCCTGCATCCGCAGCGAGGCGTTTTTTTTGCCTGCCCAGGCGGGCATACTAGGCCAGTCTCCATCCTTGGTACGCCAAACCCCATG  >  NC_002947/5762340‑5762627

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑