Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,790,150	A→G	7.4%	intergenic (+29/+39)	ynfM → / ← PP_5073	efflux transporter/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,790,150	0	A	G	7.4%	185.3 / 4.6	67	intergenic (+29/+39)	ynfM/PP_5073	efflux transporter/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (27/35);  new base G (2/3);  total (29/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.86e-01

GGCGGGGTGTTCTGGCACCAGTGGGGCTGGAACGGGATCGGGCTGTTCATTGGCAGCTTGCTGGCGGTGGCCTTGATGGTGGCGTTGCACCTGAGCAAGTTGCCACCCAAAACGGTGTGATGCATTCGTGGGC‑ACGCCCGCACCCACAACCTGTGGGAGCGGGTGTGACCACGAAGAGGCCATCAGATCAGTTGATGATCTCGACAATATCCACATCCACTTCCCGGCTCATCAGGTGCTTTTCAACCTCACCGGTCAGCTTCACCTTGGTCTTGTCGTTGAACGGTGTCGGCGGC  >  NC_002947/5790002‑5790297                                                                                                                                                      |                                                                                                                                                    ggcgggGTGTTCTGGCACCAGTGGGGCTGGAACGGGATCGGGCTGTTCATTGGCAGCTTGCTGGCGGTGGCCTTGATGGTGGAGTTGCACCTGAGCAAGTTGCCACCCAAAACGGTGTGATGCATTCGTGGGC‑ACGCCCGCACCCACaa                                                                                                                                                     >  3:166202/1‑149 (MQ=255)        tgtTCTGGCACCAGTGGGGCTGGAACGGGATCGGGCTGTTCATTGGCAGCTTGCTGGCGGTGGCCTTGATGGTGGCGTTGCACCTGAGCAAGTTGCCACCCAAAACGGTGTGATGCATTCGTGGGC‑ACGCCCGCACCCACAACCTGTgg                                                                                                                                              <  4:296245/149‑1 (MQ=255)          ttCTGGCACCAGTGGGGCTGGAACGGGATCGGGCTGTTCATTGGCAGCTTGCTGGCGGTGGCCTTGATGGTGGCGTTGCACCTGAGCAAGTTGCCACCCAAAACGGTGTGATGCATTCGTGGGC‑ACGCCCGCACCCACAACCTGTgg                                                                                                                                              >  1:324169/1‑147 (MQ=255)             tGGCACCAGTGGGGCTGGAACGGGATCGGGCTGTTCATTGGCAGCTTGCTGGCGGTGGCCTTGATGGTGGCGTTGCACCTGAGCAAGTTGCCACCCAAAACGGTGTGATGCATTCGTGGGC‑ACGCCCGCACCCACAACCTGTGGGAGCg                                                                                                                                         <  7:119331/149‑1 (MQ=255)               gCACCAGTGGGGCTGGAACGGGATCGGGCTGTTCATTGGCAGCTTGCTGGCGGTGGCCTTGATGGTGGCGTTGCACCTGAGCAAGTTGCCACCCAAAACGGTGTGATGCATTCGTGGGC‑ACGCCCGCACCCACAACCTGTGGGAGCgg                                                                                                                                        <  2:292112/148‑1 (MQ=255)               gCACCAGTGGGGCTGGAACGGGATCGGGCTGTTCATTGGCAGCTTGCTGGCGGTGGCCTTGATGGTGGCGTTGCACCTGAGCAAGTTGCCACCCAAAACGGTGTGATGCATTCGTGGGC‑ACGCCCGCACCCACAACCTGTGGGAGCgg                                                                                                                                        <  6:266499/148‑1 (MQ=255)               gCACCAGTGGGGCGGGAACGGGATCGGGCTGTTCATTGGCAGCTTGCTGGCGGTGGCCTTGATGGTGGCGTTGCACCTGAGCAAGTTGCCACCCAAAACGGTGTGATGCATTCGTGGGC‑ACACCCGCTCCCACAGGTTGTGGGTGCgg                                                                                                                                        <  8:299230/148‑1 (MQ=39)                cACCAGTGGGGCTGGAACGGGATCGGGCTGTTCATTGGCAGCTTGCTGGCGGTGGCCTTGATGGTGGCGTTGCACCTGAGCAAGTTGCCACCCAAAACGGTGTGATGCATTCGTGGGC‑ACGCCCGCACCCACAACCTGTGGGAGCGGgt                                                                                                                                      <  3:2237/149‑1 (MQ=255)                 aCCAGTGGGGCTGGAACGGGATCGGGCTGTTCATTGGCAGCTTGCTGGCGGTGGCCTTGATGGTGGCGTTGCACCTGAGCAAGTTGCCACCCAAAACGGTGTGATGCATTCGTGGTC‑ACACCCGCTCCCACAGGCTGTGGGTGCgggcg                                                                                                                                     <  8:290756/149‑3 (MQ=38)                  ccAGTGGGGCTGGAACGGGATCGGGCTGTTCATTGGCAGCTTGCTGGCGGTGGCCTTGATGGTGGCGTTGCACCTGAGCAAGTTGCCACCCAAAACGGTGTGATGCATTCGTGGGC‑ACGCCCGCACCCACAACCTGTGGGAGCGGgtgt                                                                                                                                    <  6:100688/149‑1 (MQ=255)                  ccAGTGGGGCTGGAACGGGATCGGGCTGTTCATTGGCAGCTTGCTGGCGGTGGCCTTGATGGTGGCGTTGCACCTGAGCAAGTTGCCACCCAAAACGGTGTGATGCATTCGTGGGC‑ACGCCCGCACCCACAACCTGTGGGAGCGGgtgt                                                                                                                                    <  4:234842/149‑1 (MQ=255)                      tGGGGCTGGAACGGGATCGGGCTGTTCATTGGCAGCTTGCTGGCGGTGGCCTTGATGGTGGGGTTGCACCTGAGCAAGTTGCCACCCAAAACGGTGTGATGCATTCGTGGGC‑ACGCCCGCACCCACAACCTGTGGGAGCGGGTGTGAc                                                                                                                                 <  4:166202/148‑1 (MQ=255)                            tGGAACGGGATCGGGCTGTTCATTGGCAGCTTGCTGGCGGTGGCCTTGATGGTGGCGTTGCACCTGAGCAAGTTGCCACCCAAAACGGTGTGATGCATTCGTGGGC‑ACGCCCGCACCCACAACCTGTGGGAGCGGGTGTGACCACg                                                                                                                             >  2:183945/1‑146 (MQ=255)                              gAACGGGATCGGGCTGTTCATTGGCAGCTTGCTGGCGGTGGCCTTGATGGTGGCGTTGCACCTGAGCAAGTTGCCACCCAAAACGGTGTGATGCATTCGTGGGC‑ACGCCCGCACCCACAACCTGTGGGAGCGGGTGTGACCACGAagag                                                                                                                        >  6:43832/1‑149 (MQ=255)                              gAACGGGATCGGGCTGTTCATTGGCAGCTTGCTGGCGGTGGCCTTGATGGTGGCGGTGCACCTGAGCAAGTTGCCACCCAAAACGGTGTGATGCATTCGTGGGC‑ACGCCCGCACCCACAACCTGTGGGAGCGGGTGTGACCACGAagag                                                                                                                        <  7:90621/149‑1 (MQ=255)                               aaCGGGATCGGGCTGTTCATTGGCAGCTTGCTGGCTGTTGCCTTGATGGTGGCGTTGCACCTGAGCAAGTTGCCACCCAAAACGGTGTGATGCATTCGTGGGC‑ACGCCCTAACCCACAACCTGTGGGAGCGGGTGTGACAACGAAGAgg                                                                                                                       <  4:162216/149‑1 (MQ=255)                                  gggATGGGGCTGTTCATTGGGAGCTTGCTGGCGGTGGCCTTGATGGTGGCGTTGCACCTGAGCAAGTTGCCACCCAAAACGGTGTGATGCATTCGTGGGC‑ACGCCCGCACCCACAACCTGTGGGAGCGGGTGTGACCTCGAAGAGGCCa                                                                                                                    <  7:5494/149‑1 (MQ=255)                                   ggATCGGGCTGTTCATTGGCAGCTTGCTGGCGGTGGCCTTGATGGTGGCGTTGCACCTGAGCAAGTTGCCACCCAAAACGGTGTGATGCATTCGTGGGC‑ACGCCCGCACCCACAACCTGTGGGAGCGGGTGTGACCACGAAGAGGCCat                                                                                                                   <  5:43832/149‑1 (MQ=255)                                         ggCTGTTCATTGGCAGCTTGCTGGCGGTGGCCTTGATGGTGGCGTTGCACCTGAGCAAGTTGCCACCCAAAACGGTGTGATGCATTCGTGGGC‑ACGCCCGCACCCACAACCTGTGGGAGCGGGTGTGACCACGAAGAGGCCatcacat                                                                                                              >  4:604/1‑148 (MQ=255)                                             gTTCATTGGCCGCTTGCTGCGGGTGCCCTTCTTGGTGGCGTGCAACCTCCGCATTTTGCAACCAAAAACGGGGTCATCCATTCGTGGCCAACGCCCGCACCCACAACCTGTGGGAGCGGGTGTGACCACGAAGAGGCCATCAGATCAGt                                                                                                          <  1:285387/149‑1 (MQ=255)                                                cATTGGCAGCTTGCTGGCGGTGGCCTTGATGGTGGCGTTGCACCTGAGCAAGTTGCCACCCAAAACGGTGTGATGCATTCGTGGGC‑ACGCCCGCACCCACAACCTGTGGGAg                                                                                                                                           <  6:289536/112‑1 (MQ=255)                                                cATTGGCAGCTTGCTGGCGGTGGCCTTGATGGTGGCGTTGCACCTGAGCAAGTTGCCACCCAAAACGGTGTGATGCATTCGTGGGC‑ACGCCCGCACCCACAACCTGTGGGAg                                                                                                                                           >  5:289536/1‑112 (MQ=255)                                                 aTTGGCAGCTTGCTGGCGGTGGCCTTGATGGTGGCGTTGCACCTGAGCAAGTTGCCACCCAAAACGGTGTGATGCATTCGTGGGC‑ACGCCCGCACCCACAACCTGTGGGAGCGGGTGTGACCACGAAGAGGCCATCAGATCAGtt                                                                                                         <  6:33487/145‑1 (MQ=255)                                                 aTTGGCAGCTTGCTGGCGGTGGCCTTGATGGTGGCGTTGCACCTGAGCAAGTTGCCACCCAAAACGGTGTGATGCATTCGTGGGC‑ACGCCCGCACCCACAACCTGTGGGAGCGGGTGTGACCACGAAGAGGCCATCAGATCAGtt                                                                                                         >  5:33487/1‑145 (MQ=255)                                                 aTTGGCAGCTTGCTGGCGGTGGCCTTGATGGTGGCGTTGCACCTGAGCAAGTTGCCACCCAAAACGGTGTGATGCATTCGTGGGC‑ACGCCCGCACCCACAACCTGTGGGAGCGGGTGTGACCACGAAGAGGCCATCAGATCAGTtgatg                                                                                                     <  6:182146/149‑1 (MQ=255)                                                 aTTGGCAGCTTGCTGGCGGTGGCCTTGATGGTGGCGTTGCACCTGAGCAAGTTGCCACCCAAAACGGTGTGATGCATTCGTGGGC‑ACGCCCGCACCCACAACCTGTGGGAGCGGGTGTGACCACGAAGAGGCCATCAGATCAGTtgat                                                                                                      >  6:208442/1‑148 (MQ=255)                                                 aTTGGCAGCTTGCTGGCGGTGGCCTTGAAGGTGGCGTTGCACCTGAGCAAGTTGCCACCCAAAACGGTGTGATGCATTCGTGGGC‑ACGCCCGCACCCACAACCTGTGGGAGCGGGTGTGACCACGAAGAGGCCATCAGATCAGTtgat                                                                                                      >  1:203904/1‑148 (MQ=255)                                                    ggCAGCTTGCTGGCGGTGGCCTTGATGGTGGCGTTGCACCTGAGCAAGTTGCCACCCAAAACGGTGTGATGCATTCGTGGGC‑ACGCCCGCACCCACAACCTGTgg                                                                                                                                              <  3:90178/105‑1 (MQ=255)                                                    ggCAGCTTGCTGGCGGTGGCCTTGATGGTGGCGTTGCACCTGAGCAAGTTGCCACCCAAAACGGTGTGATGCATTCGTGGGC‑ACGCCCGCACCCACAACCTGTgg                                                                                                                                              >  4:90178/1‑105 (MQ=255)                                                     gCAGCTTGCTGGCGGTGGCCTTGATGGTGGCGTTGCACCTGAGCAAGTTGCCACCCAAAACGGTGTGATGCATTCGTGGGC‑ACGCCCGCACCCACAACCTGTGGGAGCGGGTGTGACCACGAAGAGGCCATCAGATCAGTTGATGAtct                                                                                                 >  6:318175/1‑149 (MQ=255)                                                     ccAGCTTGCTGGCGGTGGCCTCGAGGGTGCCGTTGCACCGGAGCAATTTGCCACCCAAAAGGGTGGCATGCATTCGTGGGC‑ACGCCGGCACCCACAACCTGGGGGTGGGGGGGTGACCACGAAGAGGCCATCAGATCAGTTGATGAtct                                                                                                 <  8:119023/148‑1 (MQ=255)                                                         cTTGCTGGCGGTGGCCTTGATGGTGGCGTTGCACCTGAGCAAGTTGCCACCCAAAACGGTGTGATGCATTCGTGGGC‑ACGCCCGCACCCACAACCTGTGGGAGCGGGTGTGACCACGAAGAGGCCATCAGATCAGTTGATGATCTCGa                                                                                              >  8:314418/1‑148 (MQ=255)                                                                gCGGTGGCCTTGATGGTGGCGTTGCACCTGAGCAAGTTGCCACCCAAAACGGTGTGATGCATTCGTGGGC‑ACGCCCGCACCCACAACCTGTGGGAGCGGGTGTGACCACGAAGAGGCCATCAGATCAGTTGATGATCTCGACAATatcc                                                                                      <  6:70668/149‑1 (MQ=255)                                                                gCGGTGGCCTTGATGGTGGCGTTGCACCTGAGCAAGTTGCCACCCAAAACGGTGTGATGCATTCGTGGGC‑ACGCCCGCACCCACAACCTGTGGGAGCGGGTGTGACCACGAAGAGGCCATCAGATCAGTTGATGATCTCGACAATatc                                                                                       <  2:324169/148‑1 (MQ=255)                                                                      gCCTTGATGGTGGCGTTGCACCTGAGCAAGTTGCCACCCAAAACGGTGTGATGCATTCGTGGGC‑ACGCCCGCACCCACAACCTGTGGGAGCGGGTGTGACCACGAAGAGGCCATCAGATCAGTTGATGAtctc                                                                                                <  7:199956/133‑1 (MQ=255)                                                                      gCCTTGATGGTGGCGTTGCACCTGAGCAAGTTGCCACCCAAAACGGTGTGATGCATTCGTGGGC‑ACGCCCGCACCCACAACCTGTGGGAGCGGGTGTGACCACGAAGAGGCCATCAGATCAGTTGATGAtctc                                                                                                >  8:199956/1‑133 (MQ=255)                                                                        cTTGATGGTGGCGTTGCACCTGAGCAAGTTGCCACCCAAAACGGTGTGATGCATTCGTGGGC‑ACGCCCGCACCCACAACCTGTGGGAGCGGGTGTGACCACGAAGAGGCCATCAGATCAGTTGATGATCTCGACAATatccacatccac                                                                              >  3:136329/1‑149 (MQ=255)                                                                            aTGGTGGCGTTGCACCTGAGCAAGTTGCCACCCAAAACGGTGTGATGCATTCGTGGGC‑ACGCCCGCACCCACAACCTGTGGGAGCGGGTGTGACCACGAAGAGGCCATCAGATCAGTTGATGATCTCGACAATATCCACATCCACTTcc                                                                          <  1:183945/149‑1 (MQ=255)                                                                                     ttGCACCTGAGCAAGTTGCCACCCAAAACGGTGTGATGCATTCGTGGGC‑ACGCCCGCACCCACAACCTGTGGGAGCGGGTGTGACCACGAAGAGGCCATCAGATCAGtt                                                                                                         >  7:251599/1‑109 (MQ=255)                                                                                     ttGCACCTGAGCAAGTTGCCACCCAAAACGGTGTGATGCATTCGTGGGC‑ACGCCCGCACCCACAACCTGTGGGAGCGGGTGTGACCACGAAGAGGCCATCAGATCAGtt                                                                                                         <  8:251599/109‑1 (MQ=255)                                                                                     ttGCACCTGAGCAAGTTGCCACCCAAAACGGTGTGATGCATTCGTGGGC‑ACGCCCGCACCCACAACCTGTGGGAGCGGGTGTGACCACGAAGAGGCCATCAGATCAGTTGATGATCTCGa                                                                                              >  7:47956/1‑120 (MQ=255)                                                                                     ttGCACCTGAGCAAGTTGCCACCCAAAACGGTGTGATGCATTCGTGGGC‑ACGCCCGCACCCACAACCTGTGGGAGCGGGTGTGACCACGAAGAGGCCATCAGATCAGTTGATGATCTCGa                                                                                              <  8:47956/120‑1 (MQ=255)                                                                                         aCCTGAGCAAGTTGCCACCCAAAACGGTGTGATGCATTCGTGGGC‑ACGCCCGCACCCACAACCTGTGGGAGCGGGTGTGACCACGAAGAGGCCATCAGATCAGTTGATGATCTCGACAATatccacatcca                                                                               <  6:250024/131‑1 (MQ=255)                                                                                         aCCTGAGCAAGTTGCCACCCAAAACGGTGTGATGCATTCGTGGGC‑ACGCCCGCACCCACAACCTGTGGGAGCGGGTGTGACCACGAAGAGGCCATCAGATCAGTTGATGATCTCGACAATatccacatcca                                                                               >  5:250024/1‑131 (MQ=255)                                                                                                cAAGTTGCCACCCAAAACGGTGTGATGCATTCGTGGGC‑ACGCCCGCACCCACAACCTGTGGGAGCGGGTGTGACCACGAAGAGGCCATCAGATCAGTTGATGATCTCGACAATATCCACATCCACTTCCCGGCTCATCAGGTGCTTTTc                                                      <  5:208442/149‑1 (MQ=255)                                                                                                    ttGCCACCCAAAACGGTGTGATGCATTCGTGGGC‑ACGCCCGCACCCACAACCTGTGGGAGCGGGTGTGACCACGAAGAGGCCATCAGATCAGtt                                                                                                         <  7:261546/94‑1 (MQ=255)                                                                                                    ttGCCACCCAAAACGGTGTGATGCATTCGTGGGC‑ACGCCCGCACCCACAACCTGTGGGAGCGGGTGTGACCACGAAGAGGCCATCAGATCAGtt                                                                                                         >  8:261546/1‑94 (MQ=255)                                                                                                          cccAAAACGGTGTGATGCATTCGTGGGC‑ACGCCCGCACCCACAACCTGTGGGAGCGGGTGTGACCACGAAGAGGCCATCAGATCAGTTGATGATCTCGa                                                                                              >  5:368651/1‑99 (MQ=255)                                                                                                          cccAAAACGGTGTGATGCATTCGTGGGC‑ACGCCCGCACCCACAACCTGTGGGAGCGGGTGTGACCACGAAGAGGCCATCAGATCAGTTGATGATCTCGa                                                                                              <  6:368651/99‑1 (MQ=255)                                                                                                           ccAAAACGGTGTGATGCATTCGTGGGC‑ACGGCCGCACCCACAACCTGTGGGAGCGGGTGTGACCACGAAGAGGCCATCAGATCAGTTGATGATCTCGACAATATCCACATCCACTTCCCGGCTCATCAGGTGCTTTTCAACCTCACCgg                                           >  1:28478/1‑149 (MQ=255)                                                                                                           ccAAAACGGTGTGATGCATTCGTGGGC‑ACGCCCGCACCCACAACCTGTGGGAGCGGGTGTGACCACCAAGAGGCCATCAGATCAGTTGATGATCTCGACAATATCCACATCCACTTCCCGGCTCATCAGGTGCTTTTCAACCTCACCgg                                           >  5:32649/1‑149 (MQ=255)                                                                                                                      tgATGCATTCGTGGGC‑ACGCCCGCACCCACAACCTGTGGGAGCGGGTGTGACCACGAAGAGGCCATCAGATCAGTTGATGATCTCGACAATATCCACATCCACTTCCCGGCTCATCAGGTGCTTTTCAACCTCACCGGTCAGCTTCAc                                 <  2:28478/148‑1 (MQ=255)                                                                                                                        aTGCATTCGTGGGC‑ACGCCCGCACCCACAACCTGTGGGAGCGGGTGTGACAACGAAGAGGCCATCAGATCAGTTGATGATCTCGACAATATCCACATCCACTTCCCGGCTCATCAGGTGCTTTTCAACCTCACCGGTCAGCTTCACCtt                              >  2:208766/1‑149 (MQ=255)                                                                                                                          gCATTCGTGGGC‑ACGCCCGCACCCACAACCTGTGGGAGCGGGTGTGACCACGAAGAGGCCATCAGATCAGTTGATGATCTCGACAATATCCACATCCACTTCCCGGCACATCAGGTGCTTTTCAACCTCACCTGTCAGCTTCACCTTgt                            >  4:199071/1‑148 (MQ=255)                                                                                                                           tcttCGTGGTC‑ACACCCGCTCCCACAGGTTGTGGGTGCGGGCGTGCCCACGAAGAGGCCATCAGATCAGTTGATGATCTCGACAATATCCACATCCACTTCCCGGCTCATCAGGTGCTTTTCAACCTCACCGGTCAGCTTCACCTTGGt                           >  5:118594/3‑149 (MQ=25)                                                                                                                            aTTCGTGGGC‑ACGCCCGCACCCACAACCTGTGGGAGCGGGTGTGACCACGAAGAGGCCATCAGATCAGTTGATGATCTCGACAATATCCACATCCACTTCCCGGCTCATCAGGTGCTTTTCAACCTCACCGGTCAGCTTCACCTTGGTc                          <  5:318175/149‑1 (MQ=255)                                                                                                                             ttCGTGGGC‑ACGCCCGCACCCACAACCTGTGGGAGCGGGTGTGACCACGAAGAGGCCATCAGATCAGTTGATGATCTCGACAATATCCACATCCACTTCCCGGCTCATCAGGTGCTTTTCAACCTCACCGGTCAGCTTCACCTTGGTCt                         >  3:166269/1‑149 (MQ=255)                                                                                                                                gTGGGC‑ACGCCCGCACCCACAACCTGTGGGAGCGGGTGTGACCACGAAGAGGCCATCAGATCAGTTGATGATCTCGACAATATCCACATCCACTTCCCGGCTCATCAGGTGCTTTTCAACCTCACCGGTCAGCTTCACCTTGGTCTTg                       <  3:604/148‑1 (MQ=255)                                                                                                                                  gggC‑ACGCCCGCA‑CCACAACCTGTGGGAGCGGGTGTGACCACGAAGAGGCCATCAGATCAGTTGATGATCTCGACAATATCCACATCCACTTCCCGGCTCATCAGGTGCTTTTCAACCTCACCGGTCAGCTTCACCTAGGTCTTGTCGt                   >  1:291667/1‑149 (MQ=255)                                                                                                                                  gggC‑ACGCCCGCA‑CCACAACCTGTGGGAGCGGGTGTGACCACGAAGAGGCCATCAGATCAGTTGATGATCTCGACAATATCCACATCCACTTCCCGGATCATCAGGTGCTTTTCAACCTCACCGGTCAGCTTCACCTTGGTCTTGTCGt                   >  1:287348/1‑149 (MQ=255)                                                                                                                                    tc‑acaCCCGCTCCCACAGGTTGTGGGTGCGGGCGTGCCCACGAAGAGGCCATCAGATCAGTTGATGATCTCGACAATATCCACATCCACTTCCCGGCTCATCAGGTGCTTTTCAACCTCACCGGTCAGCTTCACCTTGGTCTTGTCGtt                  <  6:118594/148‑1 (MQ=38)                                                                                                                                    gC‑ACGCCCGCACCCACAACCTGTGGGAGCGGGTGTGACCACGAAGAGGCCATCAGATCAGTTGATGATCTCGACAATATCCACATCCACTTCCCGGCTCATCAGGTGCTTTTCAACCTCACCGGTCAGCTTCACCTTGGTCTTGTCGtt                  <  2:203904/149‑1 (MQ=255)                                                                                                                                            cGCTCCCACAGGTTGTGGGTGCGGGCGTGCCCACGAAGAGGCCATCAGATCAGTTGATGATCTCGACAATATCCACATCCACTTCCCGGCTCATCAGGTGCTTTTCAACCTCACCGGTCAGCTTCACCTTGGTCTTGTCGTTGAACGgt           >  7:246502/1‑149 (MQ=38)                                                                                                                                              cACCCACAACCTGTGGGAGCGGGTGTGACCACGAAGAGGCCATCAGATCAGTTGATGATCTCGACAATATCCACATCCACTTCCCGGCTCATCAGGTGCTTTTCAACCTCACCGGTCAGCTTCACCTTGGTCTTGTCGTTGAACGgtgt         <  1:208766/149‑1 (MQ=255)                                                                                                                                                 ccACAACCTGTGGTAGCGGGTGTTACCACGAATAGGCCATCAGATCAGTTTATTATCTCGACAATATCCACATCCACTTCCCGTCTCATCAGGTGCTTTTCAACCTCACCGGt                                          <  1:27823/113‑1 (MQ=255)                                                                                                                                                 ccACAACCTGTGGGAGCGGGTGTGACCACGAAGAGGCCATCAGATCAGTTGATGATCTCGACAATATCCACATCCACTTCCCGGCTCATCAGGTGCTTTTCAACCTCACCTGTCAGCTTCACCTTGGTCTTGTCGTTGAACGGTGTcgg      >  1:248566/1‑149 (MQ=255)                                                                                                                                                   acaACCTGTGGGAGCGGGTGTGACCACGAAGAGGCCATCAGATCAGTTGATGATCTCGACAATATCCACATCCACTTCCCGGCTCATCAGGTGCTTTTCAACCTCACCGGTCAGCtt                                    >  7:5128/1‑117 (MQ=255)                                                                                                                                                   acaACCTGTGGGAGCGGGTGTGACCACGAAGAGGCCATCAGATCAGTTGATGATCTCGACAATATCCACATCCACTTCCCGGCTCATCAGGTGCTTTTCAACCTCACCGGTCAGCtt                                    <  8:5128/117‑1 (MQ=255)                                                                                                                                                     aaCCTGTGGGAGCGGGTGTGACCACGAAGAGGCCATCAGATCAGTTGATGATCTCGACAATATCCACATCCACTTCCCGGCTCATCAGGTGCTTTTCAACCTCACCGGTCAGCTTCACCTTGGTCTTGTCGTTGAACGGTGTCggcggc  <  3:199071/149‑1 (MQ=255)                                                                                                                                                     aaCCTGTGGGAGCGGGTGTGACCACGAAGAGGCCATCAGATCAGTTGATGATCTCGACAATATCCACATCCACTTCCCGGCTCATCAGGTGCTTTTCAACCTCACCGGTCAGCTTCACCTTGGTCTTGTCGTTGAACGGTGTCggcggc  <  7:314418/149‑1 (MQ=255)                                                                                                                                                      |                                                                                                                                                    GGCGGGGTGTTCTGGCACCAGTGGGGCTGGAACGGGATCGGGCTGTTCATTGGCAGCTTGCTGGCGGTGGCCTTGATGGTGGCGTTGCACCTGAGCAAGTTGCCACCCAAAACGGTGTGATGCATTCGTGGGC‑ACGCCCGCACCCACAACCTGTGGGAGCGGGTGTGACCACGAAGAGGCCATCAGATCAGTTGATGATCTCGACAATATCCACATCCACTTCCCGGCTCATCAGGTGCTTTTCAACCTCACCGGTCAGCTTCACCTTGGTCTTGTCGTTGAACGGTGTCGGCGGC  >  NC_002947/5790002‑5790297

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑