Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,890,793 G→T 6.3% intergenic (+81/+39) PP_5164 → / ← plpB penicillin amidase family protein/NLPA lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,890,7930GT 6.3% 198.3 / 3.6 63intergenic (+81/+39)PP_5164/plpBpenicillin amidase family protein/NLPA lipoprotein
Reads supporting (aligned to +/- strand):  ref base G (29/29);  new base T (2/2);  total (32/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.08e-01

AGTATCAGCGGGCGCAGATGGGGGTGATTCCGGCGCAAAGTACCTTACGGCTGGTACCCCCGAAGTAAGAGGCTGCTTTGCGGCCGCTTCGCAGCAAAAGGCTTCTACGGTGCTGTGCGGGCAGGGCTGAAGAAAAACGC‑CGACACAAGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGAACGCCGGCAGTACGGCACCTTGGTACTTCTTGGCAATGAATGCCTTGACTTCCGGGCTGGTCAGGGCCTTGGCCAGTTTCTGGATGGCCTCACTGTCCTTGTT  >  NC_002947/5890645‑5890939
                                                                                                                                                     |                                                                                                                                                  
aGTATCAGCGGGCGCAGATGGGGGTGATTCCGGCGCAAAGTACCTTACGGCTGGTACCCCCGAAGTAAGAGGCTGCTTTGCGGCCGCTTCGCAGCAAAAGGCTTCTACGGTGCTGTGCGGGCAGGGCTGAAGAAAAACGC‑CGACACAAg                                                                                                                                                    >  6:228232/1‑149 (MQ=255)
  tATCAGCGGGCGCAGATGGGGGTGATTCCGGCGCAAAGTACCTTACGGCTGGTACCACCGAAGTAAGAGGCTGCTTTGCGGCCGCTTCTCAGCAAAAGGCTTCTACGGTGCTGTGCGGGCAGGGCTGAAGAAAAACGC‑CGACACAACgt                                                                                                                                                  >  5:263181/1‑149 (MQ=255)
      aGCGGGCGCAGATGGGGGTGATTCCGGCGCAAAGTACCTTACGGCTGGTACCCCCGAAGTAAGAGGCTGCTTTGCGGCCGCTTCGCAGCAAAAGGCTTCTACGGTGCTGTGCGGGCAGGGCTGAAGAAAAACGC‑CGACACCTTGTGTCg                                                                                                                                              <  5:153212/149‑1 (MQ=255)
      aGCGGGCGCAGATGGGGGTGATTCCGGCGCAAAGTACCTTACGGCTGGTACCCCCGAAGTAAGAGGCTGCTTTGCGGCCGCTTCGCAGCAAAAGGCTTCTACGGTGCTGTGCGGGCAGGGCTGAAGAAAAACGC‑CGACACAAGGTGTCg                                                                                                                                              >  6:242652/1‑149 (MQ=255)
       gCGGGCGCAGATGGGGGTGATTCCGGCGCAAAGTACCTTACGGCTGGTACCCCCGAAGTAAGAGGCTGCTTTGCGGCCGCTTCGCAGCAAAAGGCTTCTACGGTGCTGTGCGGGCAGGGCTGAAGAAAAACGC‑CGACACAAGGTGTCgg                                                                                                                                             >  2:271755/1‑149 (MQ=255)
             gcAGATGGGGGTGATTCCGGCGCAAAGTACCTTACGGCTGGTACCCCCGAAGTAAGAGGCTGCTTTGCGGCCGCTTCGCAGCAAAAGGCTTCTACGGTGCTGTGCGGGCAGGGCTGAAGAAAAACGC‑CGACACAAGGTGTCGGCGTTgg                                                                                                                                       <  8:188331/149‑1 (MQ=255)
                 aTGGGGGTGATTCCGGCGCAAAGTACCTTACGGCTGGTACCCCCGAAGTAAGAGGCTGCTTTGCGGCCGCTTCGCAGCAAAAGGCTTTTACGGTGCTGTGCGGGCAGGGCTGAAGAAAAACGC‑CGACACCTTGTGTCGGCGtttttctt                                                                                                                                   <  1:284585/149‑7 (MQ=255)
                 aTGGGGGTGATTCCGGCGCAAAGTACCTTACGGCTGGTACCCCCGAAGTAAGAGGCTGCTTTGCGGCCGCTTCGCAGCAAAAGGCTTCTACGGTGCTGTGCGGGCAGGGCTGAAGAAAAACGC‑CGACACAAGGTGTCGGCGTTGGcaca                                                                                                                                   >  3:277638/1‑149 (MQ=255)
                     gggTGATTCCGGCGCAAAGTACCTTACGGCTGGTACCCCCGAAGTAAGAGGCTGCTTTGCGGCCGCTTCGCAGCAAAAGGCTTCTACGGTGCTGTGCGGGCAGGGCTGAAGAAAAACGC‑CGACACAAGGTGTCGGCGTTGGCACatat                                                                                                                                >  3:354772/1‑148 (MQ=255)
                      ggTGATTCCGGCGCAAAGTACCTTACGGCTGGTACCCCCGAAGTAAGAGGCTGCTTTGCGGCCGCTTCGCAGC‑AAAGGCTTCTACGGTGCTGTGCGGGCAGGGCTGAAGAAAAACGC‑CGACACAAGGTGTCGGCGTTGGCACATATCAg                                                                                                                             >  6:278933/1‑149 (MQ=255)
                       gTGATTCCGGCGCAAAGTACCTTACGGCTGGTACCCCCGAAGTAAGAGGCTGCTTTGCGGCCGCTTCGCAGCAAAAGGCTTCTACGGTGCTGTGCGGGCAGGGCTGAAGAAAAACGC‑CGACACAAGGTGTCGGCGTTGGCACATATCAg                                                                                                                             <  5:242652/149‑1 (MQ=255)
                           ttCCGGCGCAAAGTACCTTACGGCTGGTACCCCCGAAGTAAGAGGCTGCTTTGCGGCCGCTTCGCAGCAAAAGGCTTCTACGGTGCTGTGCGGGCAGGGCTGAAGAAAAACGC‑CGACACAAGGTGTCg                                                                                                                                              >  1:273558/1‑128 (MQ=255)
                            tCCGGCGCAAAGTACCTTACGGCTGGTACCCCCGAAGTAAGAGGCTGCTTTGCGGCCGCTTCGCAGCAAAAGGCTTCTACGGTGCTGTGCGGGCAGGGCTGAAGAAAAACGC‑CGACACAAGGTGTCGGCGTTGGCACATATCAGGCGtt                                                                                                                        >  6:93370/1‑149 (MQ=255)
                             gcGGCTCAAAGTACCTTACTGCTGGTAACCCCGAAGTAAGAGGCTGCTTTGCGGCCGCTTCGGAGCAAAAGGCTTCTACGGTGCTTTGCGTGCAGGGCTTAAGAAAATCGC‑CGACACAAGGTGTCGGCGTTGGCAAATATCAGGCGtt                                                                                                                        <  6:263181/147‑1 (MQ=255)
                             ccGGCGCAAAGTACCTTACGGCTGGTACCCCCGAAGTAAGAGGCTGCTTTGCGGCCGCTTCGCAGCAAAAGGCTTCTACGGTGCTGTGCGGGCAGGGCTGAAGAAAAACGC‑CGACACAAGGTGTCGGCGTTGGCACATATCAGGCGTTg                                                                                                                       >  8:217958/1‑149 (MQ=255)
                                 cgcAAAGTACCTTACGGCTGGTACCCCCGAAGTAAGAGGCTGCTTTGCGGCCGCTTCGCAGCAAAAGGCTTCTACGGTGCTGTGCGGGCAGGGCTGAAGAAAAACGC‑CGACACAAGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAg                                                                                                                   <  7:53480/149‑1 (MQ=255)
                                   cAAAGTACCTTACGGCTGGTACCCCCGAAGTAAGAGGCTGCTTTGCGGCCGCTTCGCAGCAAAAGGCTTCTCCGGTGCTGTGCGGGCAGGGCTGAAGAAAAACGC‑CGACACAAGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCg                                                                                                                 <  7:94585/149‑1 (MQ=255)
                                    aaaGTACCTTACGGCTGGTACCCCCGAAGTAAGAGGCTGCTTTGCGGCCGCTTCGCAGCAAAAGGCTTCTACGGTGCTGTGCGGGCAGGGCTGAAGAAAAACGC‑CGACACAAGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCg                                                                                                                 >  3:19209/1‑148 (MQ=255)
                                      aGTACCTTACGGCTGGTACCCCCGAAGTAAGAGGCTGCTTTGCGGCCGCTTCGCAGCAAAAGGCTTCTACGGTGCTGTGCGGGCAGGGCTGAAGAAAAACGC‑CGACACAAGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGaaa                                                                                                              <  5:228232/149‑1 (MQ=255)
                                        tACCTTACGGCTGGTACCCCCGAAGTAAGAGGCTGCTTTGCGGCCGCTTCGCAGCAAAAGGCTTCTACGGTGCTGTGCGGGCAGGGCTGAAGAAAAACGC‑CGACACAAGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAAt                                                                                                             >  1:132868/1‑148 (MQ=255)
                                        tACCTTACGGCTGGTACCCCCGAAGTAAGAGGCTGCTTTGCGGCCGCTTCGCAGCAAAAGGCTTCTACGGTGCTGTGCGGGCAGGGCTGAAGAAAAACGC‑CGACACAAGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAAt                                                                                                             <  2:132868/148‑1 (MQ=255)
                                           cTTACGGCTGGTAACCCCGTAGTAAGAGGCTGCTTTGCGGCCGCTTCGCATCAAAATGCTTCTACGGTGCTTTGCGGGCATGGCTGAATAAAAACGCTCGACACAAGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAg                                                                                                          <  1:271755/149‑1 (MQ=255)
                                            ttACGGCTGGTACCCCCGAAGTAAGAGGCTGCTTTGCGGCCGCTTCGCAGCAAAAGGCTTCTACGGTGCTGTGCGGGCAGGGCTGAAGAAAAACGC‑CGACACAAGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGaa                                                                                                        >  5:121433/1‑149 (MQ=255)
                                               cGGCTGGTACCCCCGAAGTAAGAGGCTGCTTTGCGGCCGCTTCGCAGCAAAAGGCTTCTACGGTGCTGTGCGGGCAGGGCTGAAGAAAAACGC‑CGACACAAGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGAACGc                                                                                                     <  3:343133/149‑1 (MQ=255)
                                                        cccccGAAGTAAGAGGCTGCTTTGCGGCCGCTTCGCAGCAAAAGGCTTCTACGGTGCTGTGCGGGCAGGGCTGAAGAAAAACGC‑CGACACAAGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGAACGCCGGCAGTAc                                                                                            <  4:354772/149‑1 (MQ=255)
                                                         ccccGAAGTAAGAGGCTGCTTTGCGGCCGCTTCGCAGCAAAAGGCTTCTACGGTGCTGTGCGGGCAGGGCTGAAGAAAAACGC‑CGACACAAGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGAACGCCGGCAGTGCg                                                                                           <  7:217958/149‑1 (MQ=255)
                                                                        ctgcgaagCGGCCGCAAAGCAGCAAAAGGCTTCTACGGTGCTGTGCGGGCAGGGCTGAAGAAAAACGC‑CGACACAAGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGAACGCCGGCAGTACGGCACCTTGGTActtc                                                                            >  1:336698/8‑149 (MQ=255)
                                                                         tgctTTGCGGCCGCTTCGCAGCAAAAGGCTTCTACGGTGCTGTGCGGGCAGGGCTGAAGAAAAACGC‑CGACACAAGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGAACGCCGGCAGTACGGCACCTTGGTActtct                                                                           >  1:233274/1‑149 (MQ=255)
                                                                           ctTTGCGGCCGCTTCGCAGCAAAAGGCTTCTACGGTGCTGTGCGGGCAGGGCTGAAGAAAAACGC‑CGACACAAGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGAACGCCGGCAGTACGGCACCTTGGTACTTCTTg                                                                         <  5:271967/149‑1 (MQ=255)
                                                                           ctTTGCGGCCGCTTCGCAGC‑AAAGGCTTCTACGGTGCTGTGCGGGCAGGGCTGAAGAAAAACGC‑CGACACAAGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGAACGCCGGCAGTACGGCACCTTGGTActtctt                                                                          <  5:278933/147‑1 (MQ=255)
                                                                            gaagCGGCCGCAAAGCAGCAAAAGGCTTCTACGGTGCTGTGCGGGCAGGGCTGAAGAAAAACGC‑CGACACAAGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGAACGCCGGCAGTACGGCACCTTGGTACTTCTTgg                                                                        <  3:92898/146‑1 (MQ=255)
                                                                                cagCCGCTTCGCAGCAAAAGGCTTCTACGGTGCTGTGCGGGCAGGGCTGAAGAAAAACGC‑CGACACAAGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGAACGCCGGCAGTACGGCACCTTGGTACTTCTTGGCaat                                                                    <  3:135319/147‑1 (MQ=255)
                                                                                cagCCGCTTCGCAGCAAAAGGCTTCTACGGTGCTGTGCGGGCAGGGCTGAAGAAAAACGC‑CGACACAAGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGAACGCCGGCAGTACGGCACCTTGGTACTTCTTGGCaat                                                                    <  3:130163/147‑1 (MQ=255)
                                                                                cGGCCGCTTCGCAGCAAAAGGCTTCTACGGTGCTGTGCGGGCAGGGCTGAAGAAAAACGC‑CGACACAAGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGAACGCCGGCAGTACGGCACCTTGGTACTTCTTGGCaa                                                                     <  7:251992/148‑1 (MQ=255)
                                                                                              caAAAGGCTTCTACGGTGCTGTGCGGGCAGGGCTGAAGAAAAACGC‑CGACACAAGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGAACGCCGGCAGTACGGCACCTTGGTACTTCTTGGCAATGAATGCCTTGACtt                                                      >  4:144153/1‑149 (MQ=255)
                                                                                               aaaaGGCTTCTACGGTGCTGTGCGGGCAGGGCTGAAGAAAAACGC‑CGACACAAGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGAACGCCGGCAGTACGGCACCTTGGTACTTCTTGGCAATGAATGCCTTGACTTc                                                     >  8:283922/1‑149 (MQ=255)
                                                                                               aaaaGGCTTCTACGGTGCTGTGCGGGCAGGGCTGAAGAAAAACGC‑CGACACAAGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGAACGCCGGCAGTACGGCACCTTGGTACTTCTTGGCAATGAATGCCTTGACTTc                                                     >  2:69508/1‑149 (MQ=255)
                                                                                                           cGGTGCTGTGCGGGCAGGGCTGAAGAAAAACGC‑CGACACAAGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGAACGCCGGCAGTACGGCACCTTGGTACTTCTTGGCAATGAATGCCTTGACTTCCGGGCTGGTCAg                                         >  3:268891/1‑149 (MQ=255)
                                                                                                                   tgCGGGCAGGGCTGAAGAAAAACGC‑CGACACAAGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGAACGCCGGCAGTACGGCACCTTGGTACTTCTTGGCAATGAATGCCTTGACTTCCGGGCTGGTCAGGGCCTTgg                                 <  1:69508/149‑1 (MQ=255)
                                                                                                                    gCGGGCAGGGCTGAAGAAAAACGC‑CGACACAAGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGAACGCCGGCAGTACGGCACCTTGGTACTTCTTGGCAATGAATGCCTTGACTTCCGGGCTGGTCAGGGCCTTGGc                                >  6:212422/1‑149 (MQ=255)
                                                                                                                              cTGAAGAAAAACGC‑CGACACAAGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGAACGCCGGCAGTACGGCACCTTg                                                                                   >  2:347320/1‑88 (MQ=255)
                                                                                                                              cTGAAGAAAAACGC‑CGACACAAGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGAACGCCGGCAGTACGGCACCTTg                                                                                   <  1:347320/88‑1 (MQ=255)
                                                                                                                               tGAAGAAAAACGC‑CGACACAAGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGAACGCCGGCAGTACGGCACCTTGGTACTTCTTGGCAATGAATGCCTTGACTTCCGGGCTGGTCAGGGCCTTGGCCAGTTTCTgg                      >  6:359573/1‑148 (MQ=255)
                                                                                                                               tGAAGAAAAACGC‑CGACACAAGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGAACGCCGGCAGTACGGCACCTTGGTACTTCTTGGCAATGAATGCCTTGACTTCCGGGCTGGTCAGGGCCTTGGCCAGTTTCTGGa                     >  1:160562/1‑149 (MQ=255)
                                                                                                                               tGAAGAAAAACGC‑CGACACAAGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGAACGCCGGCAGTACGGCACCTTGGTACTTCTTGGCAATGAATGCCTTGACTTCCGGGCTGGTCAGGGCCTTGGCCAGTTTCTGGa                     >  2:26362/1‑149 (MQ=255)
                                                                                                                                gaagaaAAACGC‑CGACACAAGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGAACGCCGGCAGTACGGCACCTTGGTACTTCTTGGCAATGAATGCCTTGACTTCCGGGCTGGTCAGGGCCTTGGCCAGTTTCTGGAt                    >  1:339695/1‑149 (MQ=255)
                                                                                                                                 aagaaAAACGC‑CGACACAAGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGAACGCCGGCAGTACGGCACCTTGGTACTTCTTGGCAATGAATGCCTTGACTTCCGGGCTGGTCAGGGCCTTGGCCAGTTTCt                        <  5:316195/144‑1 (MQ=255)
                                                                                                                                 aagaaAAACGC‑CGACACAAGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGAACGCCGGCAGTACGGCACCTTGGTACTTCTTGGCAATGAATGCCTTGACTTCCGGGCTGGTCAGGGCCTTGGCCAGTTTCTgg                      <  5:359573/146‑1 (MQ=255)
                                                                                                                                 aagaaAAACGC‑CGACACAAGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGAACGCCGGCAGTACGGCACCTTGGTACTTCTTGGCAATGAATGCCTTGACTTCCGGGCTGGTCAGGGCCTTGGCCAGTTTCTGGa                     <  3:144153/147‑1 (MQ=255)
                                                                                                                                 aagaaAAACGC‑CGACACAAGGTGTAGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGAACGCCGGCAGTACGGCACCTTGGTACTTCTTGGCAATGAATGCCTTGACTTCCGGGCTGGTCAGGGCCTTGGCCAGTTTCt                        >  6:316195/1‑144 (MQ=255)
                                                                                                                                   gaaAAACGC‑CGACACAAGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGAACGCCGGCAGTACGGCGCCTTGGTACTTCTTGGCAATGAATGCCtt                                                           >  4:244712/1‑107 (MQ=255)
                                                                                                                                   gaaAAACGC‑CGACACAAGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGAACGCCGGCAGTACGGCACCTTGGTACTTCTTGGCAATGAATGCCtt                                                           <  3:244712/107‑1 (MQ=255)
                                                                                                                                       aaCGC‑CGACACAAGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGAACGCCGGCAGTACGGCACCTTGGTACTTCTTGGCAATGAATGCCTTGACTTCCGGGCTGGTCAGGGCCTTGGCCAGTTTCTGGATGGCCTCa             <  6:121433/149‑1 (MQ=255)
                                                                                                                                        aCGC‑CGACACCTTGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGAACGCCGGCAGTACGGCACCTTGGTACTTCTTGGCAATGAATGCCTTGACTTCCGGGCTGGTCAGGGCCTTGGCCAGTTTCTGGATGGCCTCAc            >  2:344401/1‑149 (MQ=255)
                                                                                                                                         cGC‑CGACACAAGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGAACGCCGGCAGTACGGCACCTTGGTACTTCTTGGCAATGAATGCCTTGACTTCCGGGCTGGTCAGGGCCTTGGCCAGTTTCTGGATGGCCTCACt           >  7:51884/1‑149 (MQ=255)
                                                                                                                                          gC‑CGACACCTTGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGAACGCCGGCAGTACGGCACCTTGGTACTTCTTGGCAATGAATGCCTTGACTTCCGGGCTGGTCAGGGCCTTGGCCAGTTTCTGGATGGCCTCACTg          >  6:259672/1‑149 (MQ=255)
                                                                                                                                           c‑cGACACAAGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGAACGCCGGCAGTACGGCACCTTGGTACTTCTTGGCAATGAATGCCTTGACTTCCGGGCTGGTCAGGGCCTTGGCCAGTTTCTGGATGGCCTCACTGt         >  4:170405/1‑149 (MQ=255)
                                                                                                                                             cGACACAAGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGAACGCCGGCAGTACGGCACCTTGGTACTTCTTGGCAATGAATGCCTTGACTTcc                                                    <  3:288839/105‑1 (MQ=255)
                                                                                                                                             cGACACAAGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGAACGCCGGCAGTACGGCACCTTGGTACTTCTTGGCAATGAATGCCTTGACTTcc                                                    >  4:288839/1‑105 (MQ=255)
                                                                                                                                              gACACAAGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGAACGCCGGCAGTACGGCACCTTGGTACTTCTTGGCAATGAATGCCTTGACTTCCGGGCTGGTCAGGGCCTTGGCCAGTTTCTGGATGGCCTCACTGTcc       <  7:254408/149‑1 (MQ=255)
                                                                                                                                                 acaAGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGAACGCCGGCAGTACGGCACCTTGGTACTTCTTGGCAATGAATGCCTTGACTTCCGGGCTGGTCAGGGCCTTGGCCAGTTTCTGGATGGCCTCACTGTCCttg    <  5:212422/149‑1 (MQ=255)
                                                                                                                                                 acaAGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGAACGCCGGCAGTACGGCACCTTGGTACTTCTTGGCAATGAATGCCTTGACTTCCGGGCTGGTCAGGGCCGTGGCCAGTTTCTGGATGGCCTCACTGTCCttg    <  5:93370/149‑1 (MQ=255)
                                                                                                                                                  caAGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGAACGCCGGCAGTACGGCACCTTGGTACTTCTTGGCAATGAATGCCTTGACTTCCGGGCTGGTCAGGGCCTTGGCCAGTTTCTGGATGGCCTCACTGTCCttgt   <  2:160562/149‑1 (MQ=255)
                                                                                                                                                   aaGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGAACGCCGGCAGTACGGCACCTTGGTACTTCTTGGCAATGAATGCCTTGACTTCCGGGCTGGTCAGGGCCTTGGCCAGTTTCTGGATGGCCTCACTGTCCttgtt  >  8:32961/1‑149 (MQ=255)
                                                                                                                                                     |                                                                                                                                                  
AGTATCAGCGGGCGCAGATGGGGGTGATTCCGGCGCAAAGTACCTTACGGCTGGTACCCCCGAAGTAAGAGGCTGCTTTGCGGCCGCTTCGCAGCAAAAGGCTTCTACGGTGCTGTGCGGGCAGGGCTGAAGAAAAACGC‑CGACACAAGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGAACGCCGGCAGTACGGCACCTTGGTACTTCTTGGCAATGAATGCCTTGACTTCCGGGCTGGTCAGGGCCTTGGCCAGTTTCTGGATGGCCTCACTGTCCTTGTT  >  NC_002947/5890645‑5890939

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑