Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 47,646 G→A 7.5% R577C (CGT→TGT)  czcA‑I ← cation efflux system protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00294747,6460GA 7.5% 152.1 / 3.0 53R577C (CGT→TGT) czcA‑Ication efflux system protein
Reads supporting (aligned to +/- strand):  ref base G (21/28);  new base A (2/2);  total (23/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.73e-01

GAGGCGTTCGGCGGCATCGGGTCAGATGCAATTTCTGCGGTACCCGAGCGTGCGAACATCCGCTCAACTTCAGGCACCTGCGCAATCACCGCTTTCTCCAGACGCTGCTGCATCTCGACAGATTGAGTGAGGCTCGTTCCAGGCACACGCATGGCCTGCATCGCAAAGTCACCCTCACTGAGGCTTGGGATGAACTCGCTACCCATACGACTTGCCAGCAAACCACTGAGCACGACCAAGGCTACTGCGGCCGAGAAAGCGATGTTCCGATGTCCCAGCACCCATTGCAGAACCGG  >  NC_002947/47498‑47793
                                                                                                                                                    |                                                                                                                                                   
gagGCGTTCGGCGGCATCGGGTCAGATGCAATTTCTGCGGTACCCGAGCGTGCGAACATCCGCTCAACTTCAGGCACCTGCGCAATCACCGCTTTCTCCAGACGCTGCTGCATCTCGACAGATTGAGTGAGGCTCGTTCCAGGCACACg                                                                                                                                                     >  2:159810/1‑149 (MQ=255)
   gCGTTCGGCGGCATCGGGTCAGATGCAATTTCTGCGGTACCCGAGCGTGCGAACATCCGCTCAACTTCAGGCACCTGCGCAATCACCGCTTTCTCCAGACGCTGCTGCATCTCGACAGATTGAGTGAGGCTCGTTCCAGGCACACGCAt                                                                                                                                                  <  1:121335/149‑1 (MQ=255)
   gCGTTCGGCGGCATCGGGTCAGATGCAATTTCTGCGGTACCCGAGCGTGCGAACATCCGCTCAACTTCAGGCACCTGCGCAATCACCGCTTTCTCCAGACGCTGCTGCATCTCGACAGATTGAGTGAGGCTCGTTCCAGGCACACGCAt                                                                                                                                                  <  5:146841/149‑1 (MQ=255)
         ggcggcATCGGGTCAGATGCAATTTCTGCGGTACCCGAGCGTGCGAACATCCGCTCAACTTCAGGCACCTGCGCAATCACCGCTTTCTCCAGACGCTGCTGCATCTCGACAGATTGAGTGAGGCTCGTTCCAGGCACACGCATGGCCt                                                                                                                                             <  7:344145/148‑1 (MQ=255)
             gcATCGGGTCAGATGCAATTTCTGCGGTACCCGAGCGTGCGAACATCCGCTCAACTTCAGGCACCTGCGCAATCACCGCTTTCTCCAGACGCTGCTGCATCTCGACAGATTGAGTGAGGCTCGTTCCAGGCACACGCATGGCCTGCATc                                                                                                                                        >  4:315888/1‑149 (MQ=255)
             gcATCGGGTCAGATGCAATTTCTGCGGTACCCGAGCGTGCGAACATCCGCTCAACTTCAGGCACCTGCGCAATCACCGCTTTCTCCAGACGCTGCTGCATCTCGACAGATTGAGTGAGGCTCGTTCCAGGCACACGCATGGCCTGCATc                                                                                                                                        <  5:356136/149‑1 (MQ=255)
                  gggTCAGATGCAATTTCTTCGGTACCCGAGCGTGCGAACATCCGCTCAACTTCAGGCACCTGCGCAATCACCGCTTTCTCCAGACGCTGCTGCATCTCGACAGATTGAGTGAGGCTCGTTCCAGGCACACGCATGGCCTGCATCGCaaa                                                                                                                                   <  5:177438/149‑1 (MQ=255)
                        gATGCAATTTCTGCGGTACCCGAGCGTGCGAACATCCGCTCAACTTCAGGCACCTGCGCAATCACCGCTTTCTCCAGACGCTGCTGCATCTCGACAGATTGAGTGAGGCTCGTTCCAGGCACACGCATGGCCTGCATCGCAAAGTCAcc                                                                                                                             >  3:123692/1‑149 (MQ=255)
                          tGCAATTTCTGCGGTACCCGAGCGTGCGAACATCCGCTCAACTTCAGGCACCTGCGCAATCACCGCTTTCTCCAGACGCTGCTGCATCTCGACAGATTGAGTGAGGCTCGTTCCAGGCACACGCATGGCCTGCATCGCAAAGTCACCCt                                                                                                                           >  6:133448/1‑149 (MQ=255)
                              aTTTCTGCGGTACCCGAGCGTGCGAACATCCGCTCAACTTCAGGCACCTGCGCAATCACCGCTTTCTCCAGACGCTGCTGCATCTCGACAGATTGAGTGAGGCTCGTTCCAGGCACACGCATGGCCTGCATCGCAAAGTCACCCTCACt                                                                                                                       <  4:123692/149‑1 (MQ=255)
                                      ggTACCCGAGCGTGCGAACATCCGCTCAACTTCAGTCACCTGCGCAATCACCGCTTTCTCCAGACGCTGCTGCATCTCGACAGATTGAGTGAGGCTCGTTCCAGGCACACGCATGGCCTGCATCGCAAAGTCACCCTCACTGAGGCTTg                                                                                                               <  5:245106/149‑1 (MQ=255)
                                          cccGAGCGTGCGAACATCCGCTCAACTTCAGGCACCTGCGCAATCACCGCTTTCTCCAGACGCTGCTGCATCTCGACAGATTGAGTGAGGCTCGTTCCAGGCACACGCATGGCCTGCATCGCAAAGTCACCCTCACTGAGGCTTGGGa                                                                                                            <  3:305892/148‑1 (MQ=255)
                                          cccGAGCGTGCGAACATCCGCTCAACTTCAGGCACCTGCGCAATCACCGCTTTCTCCAGACGCTGCTGCATCTCGACAGATTGAGTGAGGCTCGTTCCAGGCACACGCATGGCCTGCATCGCAAAGTCACCCTCACTGAGGCTTGGGAt                                                                                                           <  1:159810/149‑1 (MQ=255)
                                                cGTGCGAACATCCGCTCAACTTCAGGCACCTGCGCAATCACCGCTTTCTCCAGACGCTGCTGCATCTCGACAGATTGAGTGAGGCTCGTTCCAGGCACACGCATGGCCTGCATCGCAAAGTCACCCTCACTGAGGCTTGGGATGAACTc                                                                                                     <  1:113810/149‑1 (MQ=255)
                                                    cGAACATCCGCTCAACTTCAGGCACCTGCGCAATCACCGCTTTCTCCAGACGCTGCTGCATCTCGACAGATTGAGTGAGGCTCGTTCCAGGCACACGCATGGCCTGCATCGCAAAGTCACCCTCACTGAGGCTTGGGATGAACTCGCTa                                                                                                 >  1:227492/1‑149 (MQ=255)
                                                    cGAACATCCGCTCAACTTCAGGCACCTGCGCAATCACCGCTTTCTCCAGACGCTGCTGCATCTCGACAGATTGAGTGAGGCTCGTTCCAGGCACACGCATGGCCTGCATCGCAAAGTCACCCTCACTGAGGCTTGGGATGAACTCGCTa                                                                                                 <  3:315888/149‑1 (MQ=255)
                                                            cGCTCAACTTCAGGCACCTGCGCAATCACCGCTTTCTCCAGACGCTGCTGCATCTCGACAGATTGAGTGAGGCTCGTTCCAGGCACACGCATGGCCTGCATCGCAAAGTCACCCTCACTGAGGCTTGGGATGAACTCGCTACCCATAc                                                                                          <  2:227492/148‑1 (MQ=255)
                                                               tCAACTTCAGGCACCTGCGCAATCACCGCTTTCTCCAGACGCTGCTGCATCTCGACAGATTGAGTGAGGCTCGTTCCAGGCACACGCATGGc                                                                                                                                               >  7:239329/1‑92 (MQ=255)
                                                               tCAACTTCAGGCACCTGCGCAATCACCGCTTTCTCCAGACGCTGCTGCATCTCGACAGATTGAGTGAGGCTCGTTCCAGGCACACGCATGGc                                                                                                                                               <  8:239329/92‑1 (MQ=255)
                                                                  aCTTCAGGCACCTGCGCAATCACCGCTTTCTCCAGACGCTGCTGCATCTCGACAGATTGAGTGAGGCTCGTTCCAGGCACACGCATGGCCTGCATCGCAAAGTCACCCTCACTGAGGCTTGGGATGAACTCGCTACCCATACGACTTGc                                                                                   >  2:267734/1‑149 (MQ=255)
                                                                    ttCAGGCACCTGCGCAATCACCGCTTTCTCCAGACGCTGCTGCATCTCGACAGATTGAGTGAGGCTCGTTCCAGGCACACGCATGGCCTGCATCGCAAAGTCACCCTCACTGAGGTTTGGGAt                                                                                                           >  2:332386/1‑123 (MQ=255)
                                                                    ttCAGGCACCTGCGCAATCACCGCTTTCTCCAGACGCTGCTGCATCTCGACAGATTGAGTGAGGCTCGTTCCAGGCACACGCATGGCCTGCATCGCAAAGTCACCCTCACTGAGGTTTGGGAt                                                                                                           <  1:332386/123‑1 (MQ=255)
                                                                    ttCAGGCACCTGCGCAATCACCGCTTTCTCCAGACGCTGCTGCATCTCGACAGATTGAGTGAGGCTCGTTCCAGGCACACGCATGGCCTGCATCGCAAAGTCACCCTCACTGAGGCTTGGGATGAACTCGCTACCCATACGACTTGc                                                                                   >  5:134399/1‑147 (MQ=255)
                                                                      cAGGCACCTGCGCAATCACCGCTTTCTCCAGACGCTGCTGCATCTCGACAGATTGAGTGAGGCTCGTTCCAGGCACACGCATGGCCTGCATCGCAAAGTCACCCTCACTGAGGCTTGGGATGAACTCGCTACCCATACGACTTGCCa                                                                                 <  2:315880/147‑1 (MQ=255)
                                                                               gcgcAATCACCGCTTTCTCCAGACGCTGCTGCATCTCGACAGATTGAGTGAGGCTCGTTCCAGGCACACGCATGGCCTGCATCGCAAAGTCACCCTCACTGAGGCTTGGGATGAACTCGCTACCCATACGACTTGCCAGCAAACCACt                                                                       >  1:100169/1‑148 (MQ=255)
                                                                                    aTCACCGCTTTCTCCAGACGCTGCTGCATCTCGACAGATTGAGTGAGGCTCGTTCCAGGCACACGCATGGCCTGCATCGCAAAGTCACCCTCACTGAGGCTTGGGATGAACTCGCTACCCATACGACTTGCCAGCAAACCACTGAGCAc                                                                 <  3:241520/149‑1 (MQ=255)
                                                                                      cACCGCTTTCTCCAGACGCTGCTGCATCTCGACAGATTGAGTGAGGCTCGTTCCAGGCACACGCATGGCCTGCATCGCAAAGTCACCCTCACTGAGGCTTGGGATGAACTCGCTACCCATACGACTTGCCAGCAAACCACTGAGCACGa                                                               <  7:141956/149‑1 (MQ=255)
                                                                                       aCCGCTTTCTCCAGACGCTGCTGCATCTCGACAGATTGAGTGAGGCTCGTTCCATGCACACACATGGCCTGCATCGCAAAGTCACCCTCACTGAGGCTTGGGATGAACTCGCTACCCATACGACTTGCCAGCAAACCa                                                                         <  1:126264/138‑1 (MQ=255)
                                                                                       aCCGCTTTCTCCAGACGCTGCTGCATCTCGACAGATTGAGTGAGGCTCGTTCCAGGCACACGCATGGCCTGCATCGCAAAGTCACCCTCACTGAGGCTTGGGATGAACTCGCTACCCATACGACTTGCCAGCAAACCACTGAGCACGa                                                               <  6:145154/148‑1 (MQ=255)
                                                                                       aCCGCTTTCTCCAGACGCTGCTGCATCTCGACAGATTGAGTGAGGCTCGTTCCAGGCACACACATGGCCTGCATCGCAAAGTCACCCTCACTGAGGCTTGGGATGAACTCGCTACCCATACGACTTGCCAGCAAACCa                                                                         <  3:274663/138‑1 (MQ=255)
                                                                                       aCCGCTTTCTCCAGACGCTGCTGCATCTCGACAGATTGAGTGAGGCTCGTTCCAGGCACACACATGGCCTGCATCGCAAAGTCACCCTCACTGAGGCTTGGGATGAACTCGCTACCCATACGACTTGCCAGCAAACCa                                                                         >  2:126264/1‑138 (MQ=255)
                                                                                       aCCGCTTTCTCCAGACGCTGCTGCATCTCGACAGATTGAGTGAGGCTCGTTCCAGGCACACACATGGCCTGCATCGCAAAGTCACCCTCACTGAGGCTTGGGATGAACTCGCTACCCATACGACTTGCCAGCAAACCa                                                                         >  4:274663/1‑138 (MQ=255)
                                                                                           cTTTCTCCAGACGCTGCTGCATCTCGACAGATTGAGTGAGGCTCGTTCCAGGCACACGCATGGCCTGCATCGCAAAGTCACCCTCACTGAGGCTTGGGATGAACTCGCTACCCATACGACTTGCCAGCAAACCACTGAGCACGACCAAg                                                          >  1:239167/1‑149 (MQ=255)
                                                                                            tttCTCCAGACGCTGCTGCATCTCGACAGATTGAGTGAGGCTCGTTCCAGGCACACGCATGGCCTGCATCGCAAAGTCACCCTCACTGAGGCTTGGGATGAACTCGCTACCCATACGACTTGCCAGCAAACCACTGa                                                                     >  6:264624/1‑137 (MQ=255)
                                                                                            tttCTCCAGACGCTGCTGCATCTCGACAGATTGAGTGAGGCTCGTTCCAGGCACACGCATGGCCTGCATCGCAAAGTCACCCTCACTGAGGCTTGGGATGAACTCGCTACCCATACGACTTGCCAGCAAACCACTGa                                                                     <  5:264624/137‑1 (MQ=255)
                                                                                                tcCAGACGCTGCTGCATCTCGACAGATTGAGTGAGGCTCGTTCCAGGCACACGCATGGCCTGCATCGCAAAGTCACCCTCACTGAGGCTTGGGATGAACTCGCTACCCATACGACTTGCCAGCAAACCACTGAGCACGACCAAGGCTAc                                                     >  1:25994/1‑149 (MQ=255)
                                                                                                  cAGACGCTGCTGCATCTCGACAGATTGAGTGAGGCTCGTTCCAGGCACACGCATGGCCTGCATCGCAAAGTCACCCTCACTGAGGCTTGGGATGAACTCGCTACCCATACGACTTGCCAGCAAACCACTGAGCACGACCAAGGCt                                                       <  3:321049/145‑1 (MQ=255)
                                                                                                  cAGACGCTGCTGCATCTCGACAGATTGAGTGAGGCTCGTTCCAGGCACACGCATGGCCTGCATCGCAAAGTCACCCTCACTGAGGCTTGGGATGAACTCGCTACCCATACGACTTGCCAGCAAACCACTGAGCACGACCAAGGCt                                                       >  4:321049/1‑145 (MQ=255)
                                                                                                  cAGACGCTGCTGCATCTCGACAGATTGAGTGAGGCTCGTTCCAGGCACACGCATGGCCTGCATCGCAAAGTCACCCTCACTGAGGCTTGGGATGAACTCGCTACCCATACGACTTGCCAGCAAACCACTGAGCACGACCAAGGCTACt                                                    <  5:133448/148‑1 (MQ=255)
                                                                                                     aCGCTGCTGCATCTCGACAGATTGAGTGAGGCTCGTTCCAGGCACACGCATGGCCTGCATCGCAAAGTCACCCTCACTGAGGCTTGGGATGAACTCGCTACCCATACGACTTGCAAGCAAACCACTGAGCACGACCAAGGCTACTGCgg                                                >  1:55618/1‑149 (MQ=255)
                                                                                                       gctgctGCATCTCGACAGATTGAGTGAGGCTCGTTCCAGGCACACGCATGGCCTGCATCGCAAAGTCACCCTCACTGAGGCTTGGGATGAACTCGCTACCCATACGACtt                                                                                     <  3:119449/110‑1 (MQ=255)
                                                                                                       gctgctGCATCTCGACAGATTGAGTGAGGCTCGTTCCAGGCACACGCATGGCCTGCATCGCAAAGTCACCCTCACTGAGGCTTGGGATGAACTCGCTACCCATACGACtt                                                                                     >  4:119449/1‑110 (MQ=255)
                                                                                                       gctgctGCATCTCGACAGATTGAGTGAGGCTCGTTCCAGGCACACGCATGGCCTGCATCGCAAAGTCACCCTCACTGAGGCTTGGGATGAACTCGCTACCCATACGACTTGCCAGCAAACCACTGAGCACGACCAAGGCTACTGCGGcc                                              >  7:274317/1‑149 (MQ=255)
                                                                                                            tgcATCTCGACAGATTGAGTGAGGCTCGTTCCAGGCACACGCATGGCCTGCATCGCAAAGTCACCCTCACTGAGGCTTGGGATGAACTCGCTACCCATACGACTTGCCAGCAAACCa                                                                         >  2:102039/1‑117 (MQ=255)
                                                                                                            tgcATCTCGACAGATTGAGTGAGGCTCGTTCCAGGCACACGCATGGCCTGCATCGCAAAGTCACCCTCACTGAGGCTTGGGATGAACTCGCTACCCATACGACTTGCCAGCAAACCa                                                                         <  1:102039/117‑1 (MQ=255)
                                                                                                              cATCTCGACAGATTGAGTGAGGCTCGTTCCAGGCACACGCATGGCCTGCATCGCAAAGTCACCCTCACTGAGGCTTGGGATGAACTCGCTACCCATACGACTTGCCAGCAAACCACTGAGCACGACCAAGGCTACTGCGGCCGAGaaa                                        <  2:239167/148‑1 (MQ=255)
                                                                                                                          ttGAGTGAGGCTCGTTCCAGGCACACGCATGGCCTGCATCGCAAAGTCACCCTCACTGAGGCTTGGGATGAACTCGCTACCCATACGACTTGCCAGCAAATCACTGAGCACGACCAAGGCTACTGCGGCCGAGAAAGCGATGTTCCGAt                           <  1:267734/149‑1 (MQ=255)
                                                                                                                               tgagGCTCGTTCCAGGCACACGCATGGCCTGCATCGCAAAGTCACCCTCACTGAGGCTTGGGATGAACTCGCTACCCATACGACTTGCCAGCAAACCACTGAGCACGACCAAGGCTACTGCGGCCGAGAAAGCGATGTTCCGATGTcc                       >  8:176468/1‑148 (MQ=255)
                                                                                                                                    cTCGTTCCAGGCACACGCATGGCCTGCATCGCAAAGTCACCCTCACTGAGGCTTGGGATGAACTCGCTACCCATACGACTTGCCAGCAAACCACTGAGCACGACCAAGGCTACTGCGGCCGAGAAAGCGATGTTCCGATGTCCCAGCAc                 >  3:260800/1‑149 (MQ=255)
                                                                                                                                      cGTTCCAGGCACACGCATGGCCTGCATCGCAAAGTCACCCTCACTGAGGCTTGGGATGAACTCGCTACCCATACGACTTGCCAGCAAACCACTGAGCACGACCAAGGCTACTGCGGCCGAGAAAGCGATGTTCCGATGTCCCAGCAcc                >  8:222885/1‑148 (MQ=255)
                                                                                                                                          ccAGGCACACGCATGGCCTGCATCGCAAAGTCACCCTCACTGAGGCTTGGGATGAACTCGCTACCCATACGACTTGCCAGCAAACCACTGAGCACGACCAAGGCTACTGCGGCCGAGAAAGCGATGTTCCGATGTCCCAGCACCCAtt            <  1:161068/148‑1 (MQ=255)
                                                                                                                                                acacGCATGGCCTGCATCGCAAAGTCACCCTCACTGAGGCTTGGGATGAACTCGCTACCCATACGACTTGCCAGCAAACCACTGAGCACGACCAAGGCTACTGCGGCCGAGAAAGCGATGTTCCGATGTCCCAGCACCCATTGCAGAAc     <  7:222885/149‑1 (MQ=255)
                                                                                                                                                acacGCATGGCCTGCATCGCAAAGTCACCCTCACTGAGGCTTGGGATGAACTCGCTACCCATACGACTTGCCAGCAAACCACTGAGCACGACCAAGGCTACTGCGGCCGAGAAAGCGATGTTCCGATGTCCCAGCACCCATTGCAGAAc     >  7:264225/1‑149 (MQ=255)
                                                                                                                                                   cGCATGGCCTGCATCGCAAAGTCACCCTCACTGAGGCTTGGGATGAACTCGCTACCCATACGACTTGCCAGCAAACCACTGAGCACGACCAAGGCTACTGCGGCCGAGAAAGCGATGTTCCGATGTCCCAGCACCCATTGCAGAACCgg  <  2:25994/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
GAGGCGTTCGGCGGCATCGGGTCAGATGCAATTTCTGCGGTACCCGAGCGTGCGAACATCCGCTCAACTTCAGGCACCTGCGCAATCACCGCTTTCTCCAGACGCTGCTGCATCTCGACAGATTGAGTGAGGCTCGTTCCAGGCACACGCATGGCCTGCATCGCAAAGTCACCCTCACTGAGGCTTGGGATGAACTCGCTACCCATACGACTTGCCAGCAAACCACTGAGCACGACCAAGGCTACTGCGGCCGAGAAAGCGATGTTCCGATGTCCCAGCACCCATTGCAGAACCGG  >  NC_002947/47498‑47793

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑