Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,940,487 T→G 7.0% L109R (CTG→CGG)  yhhJ → translation‑like protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,940,4870TG 7.0% 236.1 / 3.1 76L109R (CTG→CGG) yhhJtranslation‑like protein
Reads supporting (aligned to +/- strand):  ref base T (34/36);  new base G (4/2);  total (38/38)
Fisher's exact test for biased strand distribution p-value = 6.74e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTGCTCTCGCGCAAGCTCAGCGAAGCCCTGCAACCGCCCGAGTTCCAGCCAGCCGTGCCGCTGCCTCCGCAGCGCCTGGACCAGGCCATGGACAGCGGCCAGTACACCTTCGTCATCAACGTGCCGGTTAACTTCCAGAGCGACCTGCTGGCCGGGCGCTCGC‑CGGAGCTGCAGATCAACGTCGATGCTACGGCCATGAGCCAAGCGTTCATGGGCGCCGGCTACATCGGCCGCATCTTCGAGCGCGAGCTGCTCGACTACAGCCAGCGTGGTAACGAGCAAAGCCCG  >  NC_002947/5940342‑5940629
                                                                                                                                                 |                                                                                                                                               
cTGCTCTCGCGCAAGCTCAGCGAAGCCCTGCAACCGCCCGAGTTCCAGCCAGCCGTGCCGCTGCCGCCGCAGCGCCTGGACCAGGGCATGGACAGCGGCCAGTAAACCTTCGTCATCAACGTGCCGGTTAACTTGCAGAGGGACcggct                                                                                                                                              >  5:224586/1‑149 (MQ=255)
cTGCTCTCGCGCAAGCTCAGAGAAGCCCTGCAACCGCCCGAGTTCCAGCCAGCCGTGCCGCTGCCTCCGCAGCGCCTGGACCAGGCCATGGACAGCGGCCAGTACACCTTCGTCATCAACGTGCCGGTTAACTTCCAGAGCGACctgct                                                                                                                                              >  3:335837/1‑149 (MQ=255)
    tctcGCGCAAGCTCAGCGAAGCCCTGCAACCGCCCGAGTTCCAGCCAGCCGTGCCGCTGCCTCCGCAGCGCCTGGACCAGGCCAAGGACAGCCGCCCGTACACCTTCGTCATCAACGTGCCGGGTAACTTCCAGAGCGACCTGCTGGcc                                                                                                                                          >  1:358061/1‑149 (MQ=255)
      tcGCGCAAGCTCAGCGAAGCCCTGCAACCGCCCGAGTTCCAGCCAGCCGTGCCGCTGCCTCCGCAGCGCCTGGACCAGGCCATGGACAGCGGCCAGTACACCTTCGTCCTCAACGTGCCGGTTAACTTCCAGAGCGACCTGCTGGCCgg                                                                                                                                        >  8:205628/1‑149 (MQ=255)
        gcgcAAGCTCAGCGAAGCCCTGCAACCGCCCGAGTTCCAGCCAGCCGTGCCGCTGCCTCCGCAGCGCCTGGACCAGGCCATGGACAGCGGCCAGTACACCTTCGTCATCAACGTGCCGGTTAACTTCCAGAGCGACCTGCTGGCCGGgc                                                                                                                                      <  4:258970/149‑1 (MQ=255)
        gcgcAAGCTCAGCGAAGCCCTGCAACCGCCCGAGTTCCAGCCAGCCGTGCCGCTGCCTCCGCAGCGCCTGGACCAGGCCATGGACAGCGGCCAGTACACCTTCGTCATCAACGTGCCGGTTAACTTCCAGAGCGACCTGCCGGCCGGgc                                                                                                                                      <  3:293726/149‑1 (MQ=255)
            aaGCTCAGCGAAGCCCTGCAACCGCCCGAGTTCCAGCCAGCCGTGCCGCTGCCTCCGCAGCGCCTGGACCAGGCCATGGACAGCGGCCAGTACACCTTCGTCATCAACGTGCCGGTTAACTTCCAGAGCGCCCGGCCAGCAGGTCGCTc                                                                                                                                  <  5:126553/149‑1 (MQ=255)
              gCTCAGCGAAGCCCTGCAACCGCCCGAGTTCCAGCCAGCCGTGCCGCTGCCTCCGCAGCGCCTGGACCAGGCCATGGACAGCGGCCAGTACACCTTCGTCATCAACGTGCCGGTTAACTTCCAGAGCGCCCGGCCAGCAGGTCGCTctg                                                                                                                                <  7:201364/149‑3 (MQ=255)
                tCAGCGAAGCCCTGCAACCGCCCGAGTTCCAGCCAGCCGCGCCGCTGCCTCCGCAGCGCCTGGACCAGGCCATGGACAGCGGCCAGTACACCTTCGTCATCAACGTGCCGGTTAACTTCCAGAGCGACCTGCTGGCCGGGCGCTCGC‑Cg                                                                                                                             >  4:139352/1‑149 (MQ=255)
                  aGCGAAGCCCTGCAACCGCCCGAGTTCCAGCCAGCCGTGCCGCTGCCTCCGCAGCGCCTGGACCAGGCCATGGACAGCGGCCAGTACACCTTCGTCATCAACGTGCCGGTTAACTTCCAGAGCGACCTGCTGGCCGGGCGCTCGC‑Cg                                                                                                                             >  6:213714/1‑147 (MQ=255)
                  aGCGAAGCCCTGCAACCGCCCGAGTTCCAGCCAGCCGTGCCGCTGCCTCCGCAGCGCCTGGACCAGGCCATGGACAGCGGCCAGTACACCTTCGTCATCAACGTGCCGGTTAACTTCCAGAGCGACCTGCTGGCCGGGCGCTCGC‑CGGa                                                                                                                           <  4:211341/149‑1 (MQ=255)
                  aGCGAAGCCCTGCAACCGCCCGAGTTCCAGCCAGCCGTGCCCCTGCCAACGCACCGCCTGGCACACGCCAAGGACAGCGGCCAGTACAACATCGTAATCAACGTGCCGGGTAACTTACAGAGCGACCGGCTGGCCCGGCGCtagacg                                                                                                                              >  6:250219/1‑142 (MQ=255)
                     gAAGCACTGCAACCGCCCGAGTTCCAGCCAGCCGTGCCGCTGCCTGCGCCGCGGCGGGACCAGGCCATGGGCAGCGGGCAGTACACCGTCGGGATGAACGTGCCGGGTGCCTTCCACAGAGCCAGGCTGGCAGGGGGCTCGC‑Cgggggt                                                                                                                        >  4:205424/1‑145 (MQ=255)
                      aaGCCCTGCAACCGCCCGAGTTCCAGCCAGCCGTGCCGCTGCCTCCGCAGCGCCTGGACCAGGCCATGGACAGCGGCCAGTACACCTTCGTCATCAACGTGCCGGTTAACTTCCAGAGCGACctgctg                                                                                                                                             <  8:43757/128‑1 (MQ=255)
                      aaGCCCTGCAACCGCCCGAGTTCCAGCCAGCCGTGCCGCTGCCTCCGCAGCGCCTGGACCAGGCCATGGACAGCGGCCAGTACACCTTCGTCATCAACGTGCCGGTTAACTTCCAGAGCGACctgctg                                                                                                                                             >  7:43757/1‑128 (MQ=255)
                      aaGCCCTGCAACCGCCCGAGTTCCAGCCAGCCGTGCCGCTGCCTCCGCAGCGCCTGGACCAGGCCATGGACAGCGGCCAGTACACCTTCGTCATCAACGTGCCGGTTAACTTCCAGAGCGACCTGCTGGCCGGGCGCTCGC‑CGGAGCTg                                                                                                                       <  2:72346/149‑1 (MQ=255)
                      aaGCCCTGCAACCGCCCGAGTTCCAGCCAGCCGTGCCGCTGCCTCCGCAGCGCCTGGACCAGGCCATGGACAGCGGCCAGTACACCTTCGTCATCAACGTGCCGGTTAACTTCCAGAGCGACCTGCTGGCCGGGCGCTCGC‑CGGAGCTg                                                                                                                       <  6:303388/149‑1 (MQ=255)
                        gCCCTGCAACCGCCCGAGTTCCAGCCAGCCGTGCCGCTGCCTCCGCAGCGCCTGGACCAGGCCATGGACAGCGGCCAGTACACCTTCGTCATCCACGTGCCGGTTAACTTCCAGAGCGACCTGCTGGCCGGGCGCTCGC‑CGGAGCTg                                                                                                                       >  7:341227/1‑147 (MQ=255)
                        gCCCTGCAACCGCCCGAGTTCCAGCCAGCCGTGCCGCTGCCTCCGCAGCGCCTGGACCAGGCCATGGACAGCGGCCAGTACACCTTCGTCATCAACGTGCCGGTTAACTTCCAGAGCGACCTGCTGGCCGGGCGCTCGC‑CGGAGCTGCa                                                                                                                     <  4:335837/149‑1 (MQ=255)
                             gCAACCGCCCGAGTTCCAGCCAGCCGTGCCGCTGCCTCCGCAGCGCCTGGACCAGGCCATGGACAGCGGCCAGTACACCTTCGTCATCAACGTGCCGGTTAACTTCCAGAGCGACCTGCTGGCCGGGCGCTCGC‑CGGAGCTGCAGATc                                                                                                                 >  2:298349/1‑148 (MQ=255)
                              cAACCGCCCGAGTTCCAGCCAGCCGTGCCGCTGCCTCCGCAGCGCCTGGACCAGGCCATGGACAGCGGCCAGTACACCTTCGTCATCAACGTGCCGGTTAACTTCCAGAGCGACCTGCTGGCCGGGCGCTCGC‑CGGAGCTGCAGATc                                                                                                                 <  5:213714/147‑1 (MQ=255)
                                  cGCCCGAGTTCCAGCCAGCCGTGCCGCTGCCTCCGCAGCGCCTGGACCAGGCCATGGACAGCGGCCAGTACACCTTCGTCATCAACGTGCCGGTTAACTTCCAGAGCGACCTGCTGGCCgg                                                                                                                                        <  8:312836/121‑1 (MQ=255)
                                  cGCCCGAGTTCCAGCCAGCCGTGCCGCTGCCTCCGCAGCGCCTGGACCAGGCCATGGACAGCGGCCAGTACACCTTCGTCATCAACGTGCCGGTTAACTTCCAGAGCGACCTGCTGGCCgg                                                                                                                                        >  7:312836/1‑121 (MQ=255)
                                      cGAGTTCCAGCCAGCCGTGCCGCTGCCTCCGCAGCGCCTGGACCAGGCCATGGACAGCGGCCAGTACACCTTCGTCATCAACGTGCCGGTTAACTTCCAGAGCGACCTGCTGGCCGGGCGCTCGC‑CGGAGCTGCAGATCAACGTCGATg                                                                                                       >  7:33742/1‑149 (MQ=255)
                                      cGAGTTCCAGCCAGCAGTGCCGCTGCCTCCGCAGCGCCTGGACCAGGCCATGGACAGCGGCCAGTACACCTTCGTCATCAACGTGCCGGTTAACTTCCAGAGCGACCTGCTGGCCGGGCGCTCGC‑CGGAGCTGCAGATCAACGTCGATg                                                                                                       <  7:156977/149‑1 (MQ=255)
                                                ccagccGTGCCGCTGCCTCCGCAGCGCCTGGACCAGGCCATGGACAGCGGCCAGTACACCTTCGTCATCAACGTGCCGGTTAACTTCCAGAGCGCCCTGCTGGCCGGGCGCTCGC‑CGGAGCTGCAGATCAACGTCGATGCTACGGCCAt                                                                                             >  7:149743/1‑149 (MQ=255)
                                                         ccGCTGCCTCCGCAGCGCCTGGACCAGGCCATGGACAGCGGCCAGTACACCTTCGTCATCAACGTGCCGGTTAACTTCCAGAGCGACCTGCTGGCCGGGCGCTCGC‑CGGAGCTGCAGATCAACGTCGATGCTACGGCCATGAGCCAAGc                                                                                    >  7:34571/1‑149 (MQ=255)
                                                           gCTGCCTCCGCAGCGCCTGGACCAGGCCATGGACAGCGGCCAGTACACCTTCGTCATCAACGTGCCGGTTAACTTCCAGAGCGACCTGCTGGCCGGGCGCTCGC‑CGGAGCTGCAGATCAACGTCGATGCTACGGCCATGAGCCAAGCGt                                                                                  <  3:139352/149‑1 (MQ=255)
                                                               ccTCCGCAGCGCCTGGACCAGGCCATGGACAGCGGCCAGTACACCTTCGTCATCAACGTGCCGGTTAACTTCCAGAGCGACCTGCTGGCCGGGCGCTCGC‑CGGAGCTGCAGATCAACGTCGATGCTACGGCCATGAGCCAAGCGTTCAt                                                                              >  6:324928/1‑149 (MQ=255)
                                                               ccTCCGCAGCGCCTGGACCAGGCCATGGACAGCGGCCAGTACACCTTCGTCATCAACGTGCCGGTTAACTTCCAGAGCGACCTGCTGGCCGGGCGCTCGC‑CGGAGCTGCAGATCAACGTCGATGCTACGGCCATGAGCCAAGCGTTCAt                                                                              <  3:117587/149‑1 (MQ=255)
                                                               ccTCCGCAGCGCCTGGACCAGGCCATGGACAGCGGCCAGTACACCTTCGTCATCAACGTGCCGGTTAACTTCCAGAGCGACCTGCTGGCCGGGCGCTCGC‑CGGAGCTGCAGATCAACGTCGATGCTACGGCCATGAGCCAAGCGTTCAt                                                                              <  8:33742/149‑1 (MQ=255)
                                                                cTCCGCAGCGCCTGGACCAGGCCATCGACAGCGGCCAGTACCCCTTCGTCATCAACGTGCCGGTTAACTTCCAGAGCGACCTGCTGGCCGGGCGCTCGC‑CGGAGCTGCAGATCAACGTCGATGCTACGGCCATGAGCCAAGCGTTCATg                                                                             <  2:358061/149‑1 (MQ=255)
                                                                 tCCGCAGCGCCTGGACCAGGCCATGGACAGCGGCCAGTACACCTTCGTCATCAACGTGCCGGTTAACTTCCAGAGCGACCTGCTGGCCGGGCGCTCGC‑CGGAGCTGCAGATCAACGTCGATGCTACGGCCATGAGCCAAGCGTTCATgg                                                                            >  4:285382/1‑149 (MQ=255)
                                                                   cGCAGCGCCTGGACCAGGCCATGGACAGCGGCCAGTACACCTTCGTCATCAACGTGCCGGTTAACTTCCAGAGCGACCTGCTGGCCGGGCGCTCGC‑CGGAGCTGCAGATCAACGTCGATGCTACGGCCATGAGCCAAGCGTTCATGGgc                                                                          >  3:305612/1‑149 (MQ=255)
                                                                        cgcCTGGACCAGGCCATGGACAGCGGCCAGTACACCTTCGTCATCAACGTGCCGGTTAACTTCCAGAGCGACCTGCTGGCCGGGCGCTCGC‑CGGAGCTGCAGATCAACGTCGATGCTACGGCCATGAGCCAAGCGTTCATGGGCGCCgg                                                                     <  8:34571/149‑1 (MQ=255)
                                                                          ccTGGACCAGGCCATGGACAGCGGCCAGTACACCTTCGTCATCAACGTGCCGGTTAACTTCCAGAGCGACCTGCTGGCCGGGCGCTCGC‑CGGAGCTGCAGATCAACGTCGATGCTACGGCCATGAGCCAAGCGTTCATGGGCGCCGGc                                                                    >  6:57285/1‑148 (MQ=255)
                                                                          ccTGGACCAGGCCATGGACAGCGGCCAGTACACCTTCGTCATCAACGTGCCGGTTAACTTCCAGAGCGACCTGCTGGCCGGGCGCTCGC‑CGGAGCTGCAGATCAACGTCGATGCTACGGCCATGAGCCAAGCGTTCATGGGCGCCGGCt                                                                   <  5:57285/149‑1 (MQ=255)
                                                                               aCCAGGCCATGGACAGCGGCCAGTACACCTTCGTCATCAACGTGCCGGTTAACTTCCAGAGCGACCTGCTGGCCGGGCGCTCGC‑CGGAGCTGCAGATCAACGTCGATGCTACGGCCATGAGCCAAGCGTTCATGGGCGCCGGCTACATc                                                              >  4:100266/1‑149 (MQ=255)
                                                                                 cAGGCCATGGACAGCGGCCAGTACACCTTCGTCATCAACGTGCCGGTTAACTTCCAGAGCGACCTGCTGGCCGGGCGCTCGC‑CGGAGCTGCAGATCAACGTCGATGCTACGGCCATGAGCCAAGCGTTCATGGGCGCCGGCtctccaag                                                            >  6:235890/1‑142 (MQ=255)
                                                                                   ggCCATGGACAGCGGCCAGTACACCTTCGTCATCAACGTGCCGGTTAACTTCCAGAGCGACCTGCTGGCCGGGCGCTCGC‑CGGAGCTGCAGATCAACGTCGATGCTACGGCCATGAGCCAAGCGTTCATGGGCGCCGGCTACATCGGcc                                                          <  3:100266/149‑1 (MQ=255)
                                                                                    gCCATGGACAGCGGCCAGTACACCTTCGTCATCAACGTGCCGGTTAACTTCCAGAGCGACCTGCTGGCCGGGCGCTCGC‑CGGAGCTGCAGATCAACGTCGATGCTACGGCCATGAGCCAAGCGTTCATg                                                                             >  5:266981/1‑129 (MQ=255)
                                                                                    gCCATGGACAGCGGCCAGTACACCTTCGTCATCAACGTGCCGGTTAACTTCCAGAGCGACCTGCTGGCCGGGCGCTCGC‑CGGAGCTGCAGATCAACGTCGATGCTACGGCCATGAGCCAAGCGTTCATg                                                                             <  6:266981/129‑1 (MQ=255)
                                                                                     ccATGGACAGCGGCCAGTACACCTTCGTCATCAACGTGCCGGTTAACTTCCAGAGCGACCTGCTGGCCGGGCGCTCGC‑CGGAGCTGCAGATCAACGTCGATGCTACGGCCATGAGCCAAGCGTTCATGGGCGCCGGCTACATCGGCCg                                                         >  1:50023/1‑148 (MQ=255)
                                                                                      cATGGACAGCGGCCAGTACACCTTCGTCATCAACGTGCCGGTTAACTTCCAGAGCGACCTGCTGGCCGGGCGCTCGC‑CGGAGCTGCAGATCAACGTCGATGCTACGGCCATGAGCCAAGCGTTCATGGGCGCCGGCTACATCGGCCGCa                                                       >  5:125376/1‑149 (MQ=255)
                                                                                      cATGGACAGCGGCCAGTACACCTTCGTCATCAACGTGCCGGTTAACTTCCAGAGCGACCTGCTGGCCGGGCGCTCGC‑CGGAGCTGCAGATCAACGTCGATGCTACGGCCATGAGCCAAGCGTTCATGGGCGCCGGCTACATCGGCCGCa                                                       >  4:133368/1‑149 (MQ=255)
                                                                                            cAGCGGCCAGTACACCTTCGTCATCAACGTGCCGGTTAACTTCCAGAGCGACCTGCTGGCCGGGCGCTCGC‑CGGAGCTGCAGATCAACGTCGATGCTACGGCCATGAGCCAAGCGTTCATGGGCGCCGGCTACATCGGCCGCATCTTCg                                                 <  3:285382/149‑1 (MQ=255)
                                                                                             aGCGGCCAGTACACCTTCGTCATCAACGTGCCGGTTAACTTCCAGAGCGACCTGCTGGCCGGGCGCTCGC‑CGGAGCTGCAGATCAACGTCGATGCTACGGCCATGAGCCAAGCGTTCATGGGCGCCGGCTACATCGGCCGCATCTTCGa                                                <  8:149743/149‑1 (MQ=255)
                                                                                             aGCGGCCAGTACACCTTCGTCATCAACGTGCCGGTTAACTTCCAGAGCGACCTGCTGGCCGGGCGCTCGC‑CGGAGCTGCAGATCAACGTCGATGCTACGGCCATGAGCCAAGCGTTCATGGGCGCCGGCTACATCGGCCGCATCTTCGa                                                <  3:98629/149‑1 (MQ=255)
                                                                                                      tACACCTTCGTCATCAACGTGCCGGTTACCTTCCAGAGCGAGCTGCTGGCCGGGCGCTCGC‑CGGAGCTGCAGATCAACGTCGATGCTACGGCCATGAGCGAAGCGTTCATGGGCGCCGGCTACATCGTCCGCATCTTCGAGCGCGAgc                                        <  5:323421/148‑1 (MQ=255)
                                                                                                      tACACCTTCGTCATCAACGTGCCGGTTAACTTCCAGAGCGACCTGCTGGCCGGGCGCTCGC‑CGGAGCTGCAGATCAACGTCGATGCTACGGCCATGAGCCAAGCGTTCATGGGCGCCGGCTACATCGGCCGCATCTTCGAGCGCGAgct                                       >  8:236963/1‑149 (MQ=255)
                                                                                                      tACACCTTCGTCATCAACGTGCCGGTTAACTTCCAGAGCGACCTGCTGGCCGGGCGCTCGC‑CGGAGCTGCAGATCAACGTCGATGCTACGGCCATGAGCCAAGCGTTCATGGGCGCCGGCTACATCGGCCGCATCTTCCAGCGCGAgc                                        >  6:323421/1‑148 (MQ=255)
                                                                                                        cacCTTCGTCATCAACGTGCCGGTTAACTTCCAGAGCGACCTGCTGGCCGGGCGCTCGC‑CGGAGCTGCAGATCAACGTCGATGCTACGGCCATGAGCCAAGCGTTCATGGGCGCCGGCTACATCGGCCGCATCTTCGAGCGCGAgctgc                                     <  6:224586/149‑1 (MQ=255)
                                                                                                          ccTTCGTCATCACCGTGCCGGTTAACTTCCAGAGCGACCTGCTGGCCGGGCGCTCGC‑CGGAGCTGCAGATCAACGTCGATGCTACGGCCATGAGCCAAGCGTTCATGGGCGCCGGCTACATCGGCCGCATCTTCGAGCGCGAGCTGCTc                                   <  7:205628/149‑1 (MQ=255)
                                                                                                          ccTTCGTCATCAACGTGCCGGTTAACTTCCAGAGCGACCTGCTGGCCGGGCGCTCGC‑CGGAGCTGCAGATCAACGTCGATGCTACGGCCATGAGCCAAGCGTTCATGGGCGCCGGCTACATCGGCCGCATCTTCGAGCGCGAGCTGCTc                                   <  8:341227/149‑1 (MQ=255)
                                                                                                           cTTCGTCATCAACGTGCCGGTTAACTTCCAGAGCGACCTGCTGGCCGGGCGCTCGC‑CGGAGCTGCAGATCAACGTCGATGCTACGGCCATGAGCCAAGCGTTCATGGGCGCCGGCTACATCGGCCGCATCTTCGAGCGCGAGCTGCTc                                   >  8:270266/1‑148 (MQ=255)
                                                                                                           cTTCGTCATCAACGTGCCGGTTAACTTCCAGAGCGACCTGCTGGCCGGGCGCTCGC‑CGGAGCTGCAGATCAACGTCGATGCTACGGCCATGAGCCAAGCGTTCATGGGCGCCGGCTACATCGGCCGCATCTTCGAGCGCGAGCTGCTc                                   >  3:129659/1‑148 (MQ=255)
                                                                                                              cGTCATCAACGTGCCGGTTAACTTCCAGAGCGACCTGCTGGCCGGGCGCTCGC‑CGGAGCTGCAGATCAACGTCGATGCTACGGCCATGAGCCAAGCGTTCATGGGCGCCGGCTACATCGGCCGCATCTTCGAGCGCGAGCTGCTCGAc                                <  7:236963/148‑1 (MQ=255)
                                                                                                                tcatcaACGTGCCGGTTAACTTCCAGAGCGACCTGCTGGCCGGGCGCTCGC‑CGGAGCTGCAGATCAACGTCGATGCTACGGCCATGAGCCAAGCGTTCATGGGCGCCGGCTACATCGGCCGCATCTTCGAGCGCGAGCTGCTCGACTAc                             <  2:50023/149‑1 (MQ=255)
                                                                                                                  atcaACGTGCCGGTTAACTTCCAGAGCGACCTGCTGGCCGGGCGCTCGCGCGATTGTGCAGATCTTCGTCGATGCTACGGCCATGAGCCAAGCGTTCATGGGCGCCGGCTACATCGGCCGCATCTTCGAGCGCGAGCTGCTCGACTAca                            <  1:298349/149‑1 (MQ=255)
                                                                                                                  atcaACGTGCCGGTTAACTTCCAGAGCGACCTGCTGGCCGGGCGCTCGC‑CGGAGCTGCAGATCAACGTCGATGCTACGGCCATGAGCCAAGCGTTCATGGGCGCCGGCTACATCGGCCGCATCTTCGAGCGCGAGCTGCTc                                   <  8:182053/141‑1 (MQ=255)
                                                                                                                  atcaACGTGCCGGTTAACTTCCAGAGCGACCTGCTGGCCGGGCGCTCGC‑CGGAGCTGCAGATCAACGTCGATGCTACGGCCATGAGCCAAGCGTTCATGGGCGCCGGCTACATCGGCCGCATCTTCGAGCGCGAGCTGCTCGACTAcag                           >  5:356315/1‑149 (MQ=255)
                                                                                                                  atcaACGTGCCGGTTAACTTCCAGAGCGACCTGCTGGCCGGGCGCTCGC‑CGGAGCTGCAGATCAACGTCGATGCTACGGCCATGAGCCAAGCGTTCATGGGCGCCGGCTACATCGGCCGCATCTTCGAGCGCGAGCTGCTCGACTAcag                           >  3:360048/1‑149 (MQ=255)
                                                                                                                  atcaACGTGCCGGTTAACTTCCAGAGCGACCTGCTGGCCGGGCGCTCGC‑CGGAGCTGCAGATCAACGTCGATGCTACGGCCATGAGCCAAGCGTTCATGGGCGCCGGCTACATCGGCCGCATCTTCGAGCGCGAGCTGCTCGACTAca                            <  3:133368/148‑1 (MQ=255)
                                                                                                                  atcaACGTGCCGGTTAACTTCCAGAGCGACCTGCTGGCCGGGCGCTCGC‑CGGAGCTGCAGATCAACGTCGATGCTACGGCCATGAGCCAAGCGTTCATGGGCGACGGCTACATCGGCCGCATCTTCGAGCGCGAGCTGCTCGagatag                            >  7:182053/1‑143 (MQ=255)
                                                                                                                     aaCGTGCCGGTTAACTTCCAGAGCGACCTGCTGGCCGGGCGCTCGC‑CGGAGCTGCAGATCAACGTCGATGCTACGGCCATGAGCCAAGCGTTCATGGGCGCCGGCTACATCGGCCGCATCTTCGAGCGCGAGCTGCTCGACTAcagcca                        <  4:305612/149‑1 (MQ=255)
                                                                                                                          gCCGGTTAACTTCCAGAGCGACCTGCTGGCCGGGCGCTCGC‑CGGAGCTGCAGATCAACGTCGATGCTACGGCCATGAGCCAAGCGTTCATGGGCGCCGGCTACATCGGCCGCATCTTCGAGCGCGAGCTGCTCGACTACAGCCAGCGTg                   >  4:356950/1‑149 (MQ=255)
                                                                                                                              gTTAACTTCCAGGGCGACCTGCTGGCCGGGCGCTCGC‑CGGAGCTGCAGATCAACGTCGATGCTACGGCCATGAGCCAAGCGTTCATGGGCGCCGGCTACATCGGCCGCATCTTCGAGCGCGAGCTGCTCGACTACAGCCAGCGTGGTaa               >  8:235904/1‑149 (MQ=255)
                                                                                                                                 aaCTTCCAGAGCGACCTGCTGGCCGGGCGCTCGC‑CGGAGCTGCAGATCAACGTCGATGCTACGGCCATGAGCCAAGCGTTCATGGGCGCCGGCTACATCGGCCGCATCTTCGAGCGCGAGCTGCTCGACTACAGCCAGCGTGGTAAc              >  6:55452/1‑147 (MQ=255)
                                                                                                                                   cTTCCAGAGCGACCTGCTGGCCGGGCGCTCGC‑CGGAGCTGCAGATCAACGTCGATGCTACGGCCATGAGCCAAGCGTTCATGGGCGCCGGCTACATCGGCCGCATCTTCGAGCGCGAGCTGCTCGACTACAGCCAGCGTGGTAACGAGc          <  7:270266/149‑1 (MQ=255)
                                                                                                                                   cTTCCAGAGCGACCTGCTGGCCGGGCGCTCGC‑CGGAGCTGCAGATCAACGTCGATGCTACGGCCATGAGCCAAGCGTTCATGGGCGCCGGCTACATCGGCCGCATCTTCGAGCGCGAGCTGCTCGACTACAGCCAGCGTGGTAACGAGc          <  6:125376/149‑1 (MQ=255)
                                                                                                                                    ttCCAGAGCGACCTGCTGGCCGGGCGCTCGC‑CGGAGCTGCAGATCAACGTCGATGCTACGGCCATGAGCCAAGCGTTCATGGGCGCCGGCTACATCGGCCGCATCTTCGAGCGCGAGCTGCTCGACTACAGCCAGCGTGGTAACGAGc          >  6:194593/1‑148 (MQ=255)
                                                                                                                                     cggcgagCGCCCGGCCAGCAGGTCGCTCGC‑CGGAGCTGCAGATCAACGTCGATGCTACGGCCATGAGCCAAGCGTTCATGGGCGCCGGCTACATCGGCCGCATCTTCGAGCGCGAGCTGCTCGACTACAGCCAGCGTGGTAACGAGCa         >  2:166226/5‑148 (MQ=255)
                                                                                                                                       gcgagCGCCCGGCCAGCAGGTCGCTCGC‑CGGAGCTGCAGATCAACGTCGATGCTACGGCCATGAGCCAAGCGTTCATGGGCGCCGGCTACATCGGCCGCATCTTCGAGCGCGAGCTGCTCGACTACAGCCAGCGTGGTAACGAGCAAAg      >  7:287907/3‑149 (MQ=255)
                                                                                                                                       cAGAGCGACCTGCTGGCCGGGCGCTCGC‑CGGAGCTGCAGATCAACGTCGATGCTACGGCCATGAGCCAAGCGTTCATGGGCGCCGGCTACATCGGCCGCATCTTCGAGCGCGAGCTGCTCGACTACAGCCAGCGTGGTAACGAGCaaa       <  5:250219/148‑1 (MQ=255)
                                                                                                                                         gagCGACCTGCTGGCCGGGCGCTCGC‑CGGAGCTGCAGATCAACGTCGATGCTACGGCCATGAGCCAAGCGTTCATGGGCGCCGGCTACATCGGCCGCATCTTCGAGCGCGAGCTGCTCGACTACAGCCAGCGTg                   <  4:2124/134‑1 (MQ=255)
                                                                                                                                         gagCGACCTGCTGGCCGGGCGCTCGC‑CGGAGCTGCAGATCAACGTCGATGCTACGGCCATGAGCCAAGCGTTCATGGGCGCCGGCTACATCGGCCGCATCTTCGAGCGCGAGCTGCTCGACTACAGCCAGCGTg                   >  3:2124/1‑134 (MQ=255)
                                                                                                                                         gagCGACCTGCTGGCCGGGCGCTCGC‑CGGAGCTGCAGATCAACGTCGATGCTACGGCCATGAGCCAAGCGTTCATGGGCGCCGGCTACATCGGCCGCATCTTCGAGCGCGAGCTGCTCGACTACAGCCAGCGTGGTAACGAGCAAAGcc    <  5:324928/149‑1 (MQ=255)
                                                                                                                                         gagCGACCTGCTGGCCGGGCGCTCGC‑CGGAGCTGCAGATCAACGTCGATGCTACGGCCATGAGCCAAGCGTTCATGGGCGCCGGCTACATCGGCCGCATCTTCGAGCGCGAGCTGCTCGACTACAGCCAGCGTGGTAACGAGCAAAGcc    >  3:205130/1‑149 (MQ=255)
                                                                                                                                         gagCGACCTGCTGGCCGGGCGCTCGC‑CGGAGCTGCAGATCAACGTCGATGCTACGGCCATGAGCCAAGCGTTCATGGGCGCCGGCTACATCGGCCGCATCTTCGAGCGCGAGCTGCTCGACTACAGCCAGCGTGGTAACGAGCAAAGcc    >  3:209785/1‑149 (MQ=255)
                                                                                                                                           gCGACCTGCTGGCCGGGCGCTCGC‑CGGAGCTGCAGATCAACGTCGATGCTACGGCCATGAGCCAAGCGTTCATGGGCGCCGGCTACATCGGCCGCATCTTCGAGCGCGAGCTGCTCGACTACAGCCAGCGTGGTAACGAGCAAAGCCCg  <  6:356315/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                               
CTGCTCTCGCGCAAGCTCAGCGAAGCCCTGCAACCGCCCGAGTTCCAGCCAGCCGTGCCGCTGCCTCCGCAGCGCCTGGACCAGGCCATGGACAGCGGCCAGTACACCTTCGTCATCAACGTGCCGGTTAACTTCCAGAGCGACCTGCTGGCCGGGCGCTCGC‑CGGAGCTGCAGATCAACGTCGATGCTACGGCCATGAGCCAAGCGTTCATGGGCGCCGGCTACATCGGCCGCATCTTCGAGCGCGAGCTGCTCGACTACAGCCAGCGTGGTAACGAGCAAAGCCCG  >  NC_002947/5940342‑5940629

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑