Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,940,494	C→A	5.9%	A111A (GCC→GCA)	yhhJ →	translation‑like protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,940,494	0	C	A	5.9%	249.1 / 3.7	77	A111A (GCC→GCA)	yhhJ	translation‑like protein
Reads supporting (aligned to +/- strand):  ref base C (35/37);  new base A (3/2);  total (38/39)
Fisher's exact test for biased strand distribution p-value = 6.75e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCTCGCGCAAGCTCAGCGAAGCCCTGCAACCGCCCGAGTTCCAGCCAGCCGTGCCGCTGCCTCCGCAGCGCCTGGACCAGGCCATGGACAGCGGCCAGTACACCTTCGTCATCAACGTGCCGGTTAACTTCCAGAGCGACCTGCTGGCCGGGCGCTCGC‑CGGAGCTGCAGATCAACGTCGATGCTACGGCCATGAGCCAAGCGTTCATGGGCGCCGGCTACATCGGCCGCATCTTCGAGCGCGAGCTGCTCGACTACAGCCAGCGTGGTAACGAGCAAAGCCCGGTGCTGATCA  >  NC_002947/5940346‑5940639                                                                                                                                                     |                                                                                                                                                   tctcGCGCAAGCTCAGCGAAGCCCTGCAACCGCCCGAGTTCCAGCCAGCCGTGCCGCTGCCTCCGCAGCGCCTGGACCAGGCCAAGGACAGCCGCCCGTACACCTTCGTCATCAACGTGCCGGGTAACTTCCAGAGCGACCTGCTGGcc                                                                                                                                                    >  1:358061/1‑149 (MQ=255)   tcGCGCAAGCTCAGCGAAGCCCTGCAACCGCCCGAGTTCCAGCCAGCCGTGCCGCTGCCTCCGCAGCGCCTGGACCAGGCCATGGACAGCGGCCAGTACACCTTCGTCCTCAACGTGCCGGTTAACTTCCAGAGCGACCTGCTGGCCgg                                                                                                                                                  >  8:205628/1‑149 (MQ=255)     gcgcAAGCTCAGCGAAGCCCTGCAACCGCCCGAGTTCCAGCCAGCCGTGCCGCTGCCTCCGCAGCGCCTGGACCAGGCCATGGACAGCGGCCAGTACACCTTCGTCATCAACGTGCCGGTTAACTTCCAGAGCGACCTGCTGGCCGGgc                                                                                                                                                <  4:258970/149‑1 (MQ=255)     gcgcAAGCTCAGCGAAGCCCTGCAACCGCCCGAGTTCCAGCCAGCCGTGCCGCTGCCTCCGCAGCGCCTGGACCAGGCCATGGACAGCGGCCAGTACACCTTCGTCATCAACGTGCCGGTTAACTTCCAGAGCGACCTGCCGGCCGGgc                                                                                                                                                <  3:293726/149‑1 (MQ=255)         aaGCTCAGCGAAGCCCTGCAACCGCCCGAGTTCCAGCCAGCCGTGCCGCTGCCTCCGCAGCGCCTGGACCAGGCCATGGACAGCGGCCAGTACACCTTCGTCATCAACGTGCCGGTTAACTTCCAGAGCGCCCGGCCAGCAGGTCGCTc                                                                                                                                            <  5:126553/149‑1 (MQ=255)           gCTCAGCGAAGCCCTGCAACCGCCCGAGTTCCAGCCAGCCGTGCCGCTGCCTCCGCAGCGCCTGGACCAGGCCATGGACAGCGGCCAGTACACCTTCGTCATCAACGTGCCGGTTAACTTCCAGAGCGCCCGGCCAGCAGGTCGCTctg                                                                                                                                          <  7:201364/149‑3 (MQ=255)             tCAGCGAAGCCCTGCAACCGCCCGAGTTCCAGCCAGCCGCGCCGCTGCCTCCGCAGCGCCTGGACCAGGCCATGGACAGCGGCCAGTACACCTTCGTCATCAACGTGCCGGTTAACTTCCAGAGCGACCTGCTGGCCGGGCGCTCGC‑Cg                                                                                                                                       >  4:139352/1‑149 (MQ=255)               aGCGAAGCCCTGCAACCGCCCGAGTTCCAGCCAGCCGTGCCGCTGCCTCCGCAGCGCCTGGACCAGGCCATGGACAGCGGCCAGTACACCTTCGTCATCAACGTGCCGGTTAACTTCCAGAGCGACCTGCTGGCCGGGCGCTCGC‑Cg                                                                                                                                       >  6:213714/1‑147 (MQ=255)               aGCGAAGCCCTGCAACCGCCCGAGTTCCAGCCAGCCGTGCCGCTGCCTCCGCAGCGCCTGGACCAGGCCATGGACAGCGGCCAGTACACCTTCGTCATCAACGTGCCGGTTAACTTCCAGAGCGACCTGCTGGCCGGGCGCTCGC‑CGGa                                                                                                                                     <  4:211341/149‑1 (MQ=255)               aGCGAAGCCCTGCAACCGCCCGAGTTCCAGCCAGCCGTGCCCCTGCCAACGCACCGCCTGGCACACGCCAAGGACAGCGGCCAGTACAACATCGTAATCAACGTGCCGGGTAACTTACAGAGCGACCGGCTGGCCCGGCGCtagacg                                                                                                                                        >  6:250219/1‑142 (MQ=255)                  gAAGCACTGCAACCGCCCGAGTTCCAGCCAGCCGTGCCGCTGCCTGCGCCGCGGCGGGACCAGGCCATGGGCAGCGGGCAGTACACCGTCGGGATGAACGTGCCGGGTGCCTTCCACAGAGCCAGGCTGGCAGGGGGCTCGC‑Cgggggt                                                                                                                                  >  4:205424/1‑145 (MQ=255)                   aaGCCCTGCAACCGCCCGAGTTCCAGCCAGCCGTGCCGCTGCCTCCGCAGCGCCTGGACCAGGCCATGGACAGCGGCCAGTACACCTTCGTCATCAACGTGCCGGTTAACTTCCAGAGCGACCTGCTGGCCGGGCGCTCGC‑CGGAGCTg                                                                                                                                 <  2:72346/149‑1 (MQ=255)                   aaGCCCTGCAACCGCCCGAGTTCCAGCCAGCCGTGCCGCTGCCTCCGCAGCGCCTGGACCAGGCCATGGACAGCGGCCAGTACACCTTCGTCATCAACGTGCCGGTTAACTTCCAGAGCGACCTGCTGGCCGGGCGCTCGC‑CGGAGCTg                                                                                                                                 <  6:303388/149‑1 (MQ=255)                     gCCCTGCAACCGCCCGAGTTCCAGCCAGCCGTGCCGCTGCCTCCGCAGCGCCTGGACCAGGCCATGGACAGCGGCCAGTACACCTTCGTCATCCACGTGCCGGTTAACTTCCAGAGCGACCTGCTGGCCGGGCGCTCGC‑CGGAGCTg                                                                                                                                 >  7:341227/1‑147 (MQ=255)                     gCCCTGCAACCGCCCGAGTTCCAGCCAGCCGTGCCGCTGCCTCCGCAGCGCCTGGACCAGGCCATGGACAGCGGCCAGTACACCTTCGTCATCAACGTGCCGGTTAACTTCCAGAGCGACCTGCTGGCCGGGCGCTCGC‑CGGAGCTGCa                                                                                                                               <  4:335837/149‑1 (MQ=255)                          gCAACCGCCCGAGTTCCAGCCAGCCGTGCCGCTGCCTCCGCAGCGCCTGGACCAGGCCATGGACAGCGGCCAGTACACCTTCGTCATCAACGTGCCGGTTAACTTCCAGAGCGACCTGCTGGCCGGGCGCTCGC‑CGGAGCTGCAGATc                                                                                                                           >  2:298349/1‑148 (MQ=255)                           cAACCGCCCGAGTTCCAGCCAGCCGTGCCGCTGCCTCCGCAGCGCCTGGACCAGGCCATGGACAGCGGCCAGTACACCTTCGTCATCAACGTGCCGGTTAACTTCCAGAGCGACCTGCTGGCCGGGCGCTCGC‑CGGAGCTGCAGATc                                                                                                                           <  5:213714/147‑1 (MQ=255)                               cGCCCGAGTTCCAGCCAGCCGTGCCGCTGCCTCCGCAGCGCCTGGACCAGGCCATGGACAGCGGCCAGTACACCTTCGTCATCAACGTGCCGGTTAACTTCCAGAGCGACCTGCTGGCCgg                                                                                                                                                  >  7:312836/1‑121 (MQ=255)                               cGCCCGAGTTCCAGCCAGCCGTGCCGCTGCCTCCGCAGCGCCTGGACCAGGCCATGGACAGCGGCCAGTACACCTTCGTCATCAACGTGCCGGTTAACTTCCAGAGCGACCTGCTGGCCgg                                                                                                                                                  <  8:312836/121‑1 (MQ=255)                                   cGAGTTCCAGCCAGCCGTGCCGCTGCCTCCGCAGCGCCTGGACCAGGCCATGGACAGCGGCCAGTACACCTTCGTCATCAACGTGCCGGTTAACTTCCAGAGCGACCTGCTGGCCGGGCGCTCGC‑CGGAGCTGCAGATCAACGTCGATg                                                                                                                 >  7:33742/1‑149 (MQ=255)                                   cGAGTTCCAGCCAGCAGTGCCGCTGCCTCCGCAGCGCCTGGACCAGGCCATGGACAGCGGCCAGTACACCTTCGTCATCAACGTGCCGGTTAACTTCCAGAGCGACCTGCTGGCCGGGCGCTCGC‑CGGAGCTGCAGATCAACGTCGATg                                                                                                                 <  7:156977/149‑1 (MQ=255)                                             ccagccGTGCCGCTGCCTCCGCAGCGCCTGGACCAGGCCATGGACAGCGGCCAGTACACCTTCGTCATCAACGTGCCGGTTAACTTCCAGAGCGCCCTGCTGGCCGGGCGCTCGC‑CGGAGCTGCAGATCAACGTCGATGCTACGGCCAt                                                                                                       >  7:149743/1‑149 (MQ=255)                                                      ccGCTGCCTCCGCAGCGCCTGGACCAGGCCATGGACAGCGGCCAGTACACCTTCGTCATCAACGTGCCGGTTAACTTCCAGAGCGACCTGCTGGCCGGGCGCTCGC‑CGGAGCTGCAGATCAACGTCGATGCTACGGCCATGAGCCAAGc                                                                                              >  7:34571/1‑149 (MQ=255)                                                        gCTGCCTCCGCAGCGCCTGGACCAGGCCATGGACAGCGGCCAGTACACCTTCGTCATCAACGTGCCGGTTAACTTCCAGAGCGACCTGCTGGCCGGGCGCTCGC‑CGGAGCTGCAGATCAACGTCGATGCTACGGCCATGAGCCAAGCGt                                                                                            <  3:139352/149‑1 (MQ=255)                                                            ccTCCGCAGCGCCTGGACCAGGCCATGGACAGCGGCCAGTACACCTTCGTCATCAACGTGCCGGTTAACTTCCAGAGCGACCTGCTGGCCGGGCGCTCGC‑CGGAGCTGCAGATCAACGTCGATGCTACGGCCATGAGCCAAGCGTTCAt                                                                                        >  6:324928/1‑149 (MQ=255)                                                            ccTCCGCAGCGCCTGGACCAGGCCATGGACAGCGGCCAGTACACCTTCGTCATCAACGTGCCGGTTAACTTCCAGAGCGACCTGCTGGCCGGGCGCTCGC‑CGGAGCTGCAGATCAACGTCGATGCTACGGCCATGAGCCAAGCGTTCAt                                                                                        <  3:117587/149‑1 (MQ=255)                                                            ccTCCGCAGCGCCTGGACCAGGCCATGGACAGCGGCCAGTACACCTTCGTCATCAACGTGCCGGTTAACTTCCAGAGCGACCTGCTGGCCGGGCGCTCGC‑CGGAGCTGCAGATCAACGTCGATGCTACGGCCATGAGCCAAGCGTTCAt                                                                                        <  8:33742/149‑1 (MQ=255)                                                             cTCCGCAGCGCCTGGACCAGGCCATCGACAGCGGCCAGTACCCCTTCGTCATCAACGTGCCGGTTAACTTCCAGAGCGACCTGCTGGCCGGGCGCTCGC‑CGGAGCTGCAGATCAACGTCGATGCTACGGCCATGAGCCAAGCGTTCATg                                                                                       <  2:358061/149‑1 (MQ=255)                                                              tCCGCAGCGCCTGGACCAGGCCATGGACAGCGGCCAGTACACCTTCGTCATCAACGTGCCGGTTAACTTCCAGAGCGACCTGCTGGCCGGGCGCTCGC‑CGGAGCTGCAGATCAACGTCGATGCTACGGCCATGAGCCAAGCGTTCATgg                                                                                      >  4:285382/1‑149 (MQ=255)                                                                cGCAGCGCCTGGACCAGGCCATGGACAGCGGCCAGTACACCTTCGTCATCAACGTGCCGGTTAACTTCCAGAGCGACCTGCTGGCCGGGCGCTCGC‑CGGAGCTGCAGATCAACGTCGATGCTACGGCCATGAGCCAAGCGTTCATGGgc                                                                                    >  3:305612/1‑149 (MQ=255)                                                                     cgcCTGGACCAGGCCATGGACAGCGGCCAGTACACCTTCGTCATCAACGTGCCGGTTAACTTCCAGAGCGACCTGCTGGCCGGGCGCTCGC‑CGGAGCTGCAGATCAACGTCGATGCTACGGCCATGAGCCAAGCGTTCATGGGCGCCgg                                                                               <  8:34571/149‑1 (MQ=255)                                                                       ccTGGACCAGGCCATGGACAGCGGCCAGTACACCTTCGTCATCAACGTGCCGGTTAACTTCCAGAGCGACCTGCTGGCCGGGCGCTCGC‑CGGAGCTGCAGATCAACGTCGATGCTACGGCCATGAGCCAAGCGTTCATGGGCGCCGGc                                                                              >  6:57285/1‑148 (MQ=255)                                                                       ccTGGACCAGGCCATGGACAGCGGCCAGTACACCTTCGTCATCAACGTGCCGGTTAACTTCCAGAGCGACCTGCTGGCCGGGCGCTCGC‑CGGAGCTGCAGATCAACGTCGATGCTACGGCCATGAGCCAAGCGTTCATGGGCGCCGGCt                                                                             <  5:57285/149‑1 (MQ=255)                                                                            aCCAGGCCATGGACAGCGGCCAGTACACCTTCGTCATCAACGTGCCGGTTAACTTCCAGAGCGACCTGCTGGCCGGGCGCTCGC‑CGGAGCTGCAGATCAACGTCGATGCTACGGCCATGAGCCAAGCGTTCATGGGCGCCGGCTACATc                                                                        >  4:100266/1‑149 (MQ=255)                                                                              cAGGCCATGGACAGCGGCCAGTACACCTTCGTCATCAACGTGCCGGTTAACTTCCAGAGCGACCTGCTGGCCGGGCGCTCGC‑CGGAGCTGCAGATCAACGTCGATGCTACGGCCATGAGCCAAGCGTTCATGGGCGCCGGCtctccaag                                                                      >  6:235890/1‑142 (MQ=255)                                                                                ggCCATGGACAGCGGCCAGTACACCTTCGTCATCAACGTGCCGGTTAACTTCCAGAGCGACCTGCTGGCCGGGCGCTCGC‑CGGAGCTGCAGATCAACGTCGATGCTACGGCCATGAGCCAAGCGTTCATGGGCGCCGGCTACATCGGcc                                                                    <  3:100266/149‑1 (MQ=255)                                                                                 gCCATGGACAGCGGCCAGTACACCTTCGTCATCAACGTGCCGGTTAACTTCCAGAGCGACCTGCTGGCCGGGCGCTCGC‑CGGAGCTGCAGATCAACGTCGATGCTACGGCCATGAGCCAAGCGTTCATg                                                                                       <  6:266981/129‑1 (MQ=255)                                                                                 gCCATGGACAGCGGCCAGTACACCTTCGTCATCAACGTGCCGGTTAACTTCCAGAGCGACCTGCTGGCCGGGCGCTCGC‑CGGAGCTGCAGATCAACGTCGATGCTACGGCCATGAGCCAAGCGTTCATg                                                                                       >  5:266981/1‑129 (MQ=255)                                                                                  ccATGGACAGCGGCCAGTACACCTTCGTCATCAACGTGCCGGTTAACTTCCAGAGCGACCTGCTGGCCGGGCGCTCGC‑CGGAGCTGCAGATCAACGTCGATGCTACGGCCATGAGCCAAGCGTTCATGGGCGCCGGCTACATCGGCCg                                                                   >  1:50023/1‑148 (MQ=255)                                                                                   cATGGACAGCGGCCAGTACACCTTCGTCATCAACGTGCCGGTTAACTTCCAGAGCGACCTGCTGGCCGGGCGCTCGC‑CGGAGCTGCAGATCAACGTCGATGCTACGGCCATGAGCCAAGCGTTCATGGGCGCCGGCTACATCGGCCGCa                                                                 >  5:125376/1‑149 (MQ=255)                                                                                   cATGGACAGCGGCCAGTACACCTTCGTCATCAACGTGCCGGTTAACTTCCAGAGCGACCTGCTGGCCGGGCGCTCGC‑CGGAGCTGCAGATCAACGTCGATGCTACGGCCATGAGCCAAGCGTTCATGGGCGCCGGCTACATCGGCCGCa                                                                 >  4:133368/1‑149 (MQ=255)                                                                                         cAGCGGCCAGTACACCTTCGTCATCAACGTGCCGGTTAACTTCCAGAGCGACCTGCTGGCCGGGCGCTCGC‑CGGAGCTGCAGATCAACGTCGATGCTACGGCCATGAGCCAAGCGTTCATGGGCGCCGGCTACATCGGCCGCATCTTCg                                                           <  3:285382/149‑1 (MQ=255)                                                                                          aGCGGCCAGTACACCTTCGTCATCAACGTGCCGGTTAACTTCCAGAGCGACCTGCTGGCCGGGCGCTCGC‑CGGAGCTGCAGATCAACGTCGATGCTACGGCCATGAGCCAAGCGTTCATGGGCGCCGGCTACATCGGCCGCATCTTCGa                                                          <  8:149743/149‑1 (MQ=255)                                                                                          aGCGGCCAGTACACCTTCGTCATCAACGTGCCGGTTAACTTCCAGAGCGACCTGCTGGCCGGGCGCTCGC‑CGGAGCTGCAGATCAACGTCGATGCTACGGCCATGAGCCAAGCGTTCATGGGCGCCGGCTACATCGGCCGCATCTTCGa                                                          <  3:98629/149‑1 (MQ=255)                                                                                                   tACACCTTCGTCATCAACGTGCCGGTTACCTTCCAGAGCGAGCTGCTGGCCGGGCGCTCGC‑CGGAGCTGCAGATCAACGTCGATGCTACGGCCATGAGCGAAGCGTTCATGGGCGCCGGCTACATCGTCCGCATCTTCGAGCGCGAgc                                                  <  5:323421/148‑1 (MQ=255)                                                                                                   tACACCTTCGTCATCAACGTGCCGGTTAACTTCCAGAGCGACCTGCTGGCCGGGCGCTCGC‑CGGAGCTGCAGATCAACGTCGATGCTACGGCCATGAGCCAAGCGTTCATGGGCGCCGGCTACATCGGCCGCATCTTCGAGCGCGAgct                                                 >  8:236963/1‑149 (MQ=255)                                                                                                   tACACCTTCGTCATCAACGTGCCGGTTAACTTCCAGAGCGACCTGCTGGCCGGGCGCTCGC‑CGGAGCTGCAGATCAACGTCGATGCTACGGCCATGAGCCAAGCGTTCATGGGCGCCGGCTACATCGGCCGCATCTTCCAGCGCGAgc                                                  >  6:323421/1‑148 (MQ=255)                                                                                                     cacCTTCGTCATCAACGTGCCGGTTAACTTCCAGAGCGACCTGCTGGCCGGGCGCTCGC‑CGGAGCTGCAGATCAACGTCGATGCTACGGCCATGAGCCAAGCGTTCATGGGCGCCGGCTACATCGGCCGCATCTTCGAGCGCGAgctgc                                               <  6:224586/149‑1 (MQ=255)                                                                                                       ccTTCGTCATCACCGTGCCGGTTAACTTCCAGAGCGACCTGCTGGCCGGGCGCTCGC‑CGGAGCTGCAGATCAACGTCGATGCTACGGCCATGAGCCAAGCGTTCATGGGCGCCGGCTACATCGGCCGCATCTTCGAGCGCGAGCTGCTc                                             <  7:205628/149‑1 (MQ=255)                                                                                                       ccTTCGTCATCAACGTGCCGGTTAACTTCCAGAGCGACCTGCTGGCCGGGCGCTCGC‑CGGAGCTGCAGATCAACGTCGATGCTACGGCCATGAGCCAAGCGTTCATGGGCGCCGGCTACATCGGCCGCATCTTCGAGCGCGAGCTGCTc                                             <  8:341227/149‑1 (MQ=255)                                                                                                        cTTCGTCATCAACGTGCCGGTTAACTTCCAGAGCGACCTGCTGGCCGGGCGCTCGC‑CGGAGCTGCAGATCAACGTCGATGCTACGGCCATGAGCCAAGCGTTCATGGGCGCCGGCTACATCGGCCGCATCTTCGAGCGCGAGCTGCTc                                             >  8:270266/1‑148 (MQ=255)                                                                                                        cTTCGTCATCAACGTGCCGGTTAACTTCCAGAGCGACCTGCTGGCCGGGCGCTCGC‑CGGAGCTGCAGATCAACGTCGATGCTACGGCCATGAGCCAAGCGTTCATGGGCGCCGGCTACATCGGCCGCATCTTCGAGCGCGAGCTGCTc                                             >  3:129659/1‑148 (MQ=255)                                                                                                           cGTCATCAACGTGCCGGTTAACTTCCAGAGCGACCTGCTGGCCGGGCGCTCGC‑CGGAGCTGCAGATCAACGTCGATGCTACGGCCATGAGCCAAGCGTTCATGGGCGCCGGCTACATCGGCCGCATCTTCGAGCGCGAGCTGCTCGAc                                          <  7:236963/148‑1 (MQ=255)                                                                                                             tcatcaACGTGCCGGTTAACTTCCAGAGCGACCTGCTGGCCGGGCGCTCGC‑CGGAGCTGCAGATCAACGTCGATGCTACGGCCATGAGCCAAGCGTTCATGGGCGCCGGCTACATCGGCCGCATCTTCGAGCGCGAGCTGCTCGACTAc                                       <  2:50023/149‑1 (MQ=255)                                                                                                               atcaACGTGCCGGTTAACTTCCAGAGCGACCTGCTGGCCGGGCGCTCGCGCGATTGTGCAGATCTTCGTCGATGCTACGGCCATGAGCCAAGCGTTCATGGGCGCCGGCTACATCGGCCGCATCTTCGAGCGCGAGCTGCTCGACTAca                                      <  1:298349/149‑1 (MQ=255)                                                                                                               atcaACGTGCCGGTTAACTTCCAGAGCGACCTGCTGGCCGGGCGCTCGC‑CGGAGCTGCAGATCAACGTCGATGCTACGGCCATGAGCCAAGCGTTCATGGGCGCCGGCTACATCGGCCGCATCTTCGAGCGCGAGCTGCTc                                             <  8:182053/141‑1 (MQ=255)                                                                                                               atcaACGTGCCGGTTAACTTCCAGAGCGACCTGCTGGCCGGGCGCTCGC‑CGGAGCTGCAGATCAACGTCGATGCTACGGCCATGAGCCAAGCGTTCATGGGCGCCGGCTACATCGGCCGCATCTTCGAGCGCGAGCTGCTCGACTAcag                                     >  3:360048/1‑149 (MQ=255)                                                                                                               atcaACGTGCCGGTTAACTTCCAGAGCGACCTGCTGGCCGGGCGCTCGC‑CGGAGCTGCAGATCAACGTCGATGCTACGGCCATGAGCCAAGCGTTCATGGGCGCCGGCTACATCGGCCGCATCTTCGAGCGCGAGCTGCTCGACTAcag                                     >  5:356315/1‑149 (MQ=255)                                                                                                               atcaACGTGCCGGTTAACTTCCAGAGCGACCTGCTGGCCGGGCGCTCGC‑CGGAGCTGCAGATCAACGTCGATGCTACGGCCATGAGCCAAGCGTTCATGGGCGCCGGCTACATCGGCCGCATCTTCGAGCGCGAGCTGCTCGACTAca                                      <  3:133368/148‑1 (MQ=255)                                                                                                               atcaACGTGCCGGTTAACTTCCAGAGCGACCTGCTGGCCGGGCGCTCGC‑CGGAGCTGCAGATCAACGTCGATGCTACGGCCATGAGCCAAGCGTTCATGGGCGACGGCTACATCGGCCGCATCTTCGAGCGCGAGCTGCTCGagatag                                      >  7:182053/1‑143 (MQ=255)                                                                                                                  aaCGTGCCGGTTAACTTCCAGAGCGACCTGCTGGCCGGGCGCTCGC‑CGGAGCTGCAGATCAACGTCGATGCTACGGCCATGAGCCAAGCGTTCATGGGCGCCGGCTACATCGGCCGCATCTTCGAGCGCGAGCTGCTCGACTAcagcca                                  <  4:305612/149‑1 (MQ=255)                                                                                                                       gCCGGTTAACTTCCAGAGCGACCTGCTGGCCGGGCGCTCGC‑CGGAGCTGCAGATCAACGTCGATGCTACGGCCATGAGCCAAGCGTTCATGGGCGCCGGCTACATCGGCCGCATCTTCGAGCGCGAGCTGCTCGACTACAGCCAGCGTg                             >  4:356950/1‑149 (MQ=255)                                                                                                                           gTTAACTTCCAGGGCGACCTGCTGGCCGGGCGCTCGC‑CGGAGCTGCAGATCAACGTCGATGCTACGGCCATGAGCCAAGCGTTCATGGGCGCCGGCTACATCGGCCGCATCTTCGAGCGCGAGCTGCTCGACTACAGCCAGCGTGGTaa                         >  8:235904/1‑149 (MQ=255)                                                                                                                              aaCTTCCAGAGCGACCTGCTGGCCGGGCGCTCGC‑CGGAGCTGCAGATCAACGTCGATGCTACGGCCATGAGCCAAGCGTTCATGGGCGCCGGCTACATCGGCCGCATCTTCGAGCGCGAGCTGCTCGACTACAGCCAGCGTGGTAAc                        >  6:55452/1‑147 (MQ=255)                                                                                                                                cTTCCAGAGCGACCTGCTGGCCGGGCGCTCGC‑CGGAGCTGCAGATCAACGTCGATGCTACGGCCATGAGCCAAGCGTTCATGGGCGCCGGCTACATCGGCCGCATCTTCGAGCGCGAGCTGCTCGACTACAGCCAGCGTGGTAACGAGc                    <  7:270266/149‑1 (MQ=255)                                                                                                                                cTTCCAGAGCGACCTGCTGGCCGGGCGCTCGC‑CGGAGCTGCAGATCAACGTCGATGCTACGGCCATGAGCCAAGCGTTCATGGGCGCCGGCTACATCGGCCGCATCTTCGAGCGCGAGCTGCTCGACTACAGCCAGCGTGGTAACGAGc                    <  6:125376/149‑1 (MQ=255)                                                                                                                                 ttCCAGAGCGACCTGCTGGCCGGGCGCTCGC‑CGGAGCTGCAGATCAACGTCGATGCTACGGCCATGAGCCAAGCGTTCATGGGCGCCGGCTACATCGGCCGCATCTTCGAGCGCGAGCTGCTCGACTACAGCCAGCGTGGTAACGAGc                    >  6:194593/1‑148 (MQ=255)                                                                                                                                  cggcgagCGCCCGGCCAGCAGGTCGCTCGC‑CGGAGCTGCAGATCAACGTCGATGCTACGGCCATGAGCCAAGCGTTCATGGGCGCCGGCTACATCGGCCGCATCTTCGAGCGCGAGCTGCTCGACTACAGCCAGCGTGGTAACGAGCa                   >  2:166226/5‑148 (MQ=255)                                                                                                                                    gcgagCGCCCGGCCAGCAGGTCGCTCGC‑CGGAGCTGCAGATCAACGTCGATGCTACGGCCATGAGCCAAGCGTTCATGGGCGCCGGCTACATCGGCCGCATCTTCGAGCGCGAGCTGCTCGACTACAGCCAGCGTGGTAACGAGCAAAg                >  7:287907/3‑149 (MQ=255)                                                                                                                                    cAGAGCGACCTGCTGGCCGGGCGCTCGC‑CGGAGCTGCAGATCAACGTCGATGCTACGGCCATGAGCCAAGCGTTCATGGGCGCCGGCTACATCGGCCGCATCTTCGAGCGCGAGCTGCTCGACTACAGCCAGCGTGGTAACGAGCaaa                 <  5:250219/148‑1 (MQ=255)                                                                                                                                      gagCGACCTGCTGGCCGGGCGCTCGC‑CGGAGCTGCAGATCAACGTCGATGCTACGGCCATGAGCCAAGCGTTCATGGGCGCCGGCTACATCGGCCGCATCTTCGAGCGCGAGCTGCTCGACTACAGCCAGCGTg                             >  3:2124/1‑134 (MQ=255)                                                                                                                                      gagCGACCTGCTGGCCGGGCGCTCGC‑CGGAGCTGCAGATCAACGTCGATGCTACGGCCATGAGCCAAGCGTTCATGGGCGCCGGCTACATCGGCCGCATCTTCGAGCGCGAGCTGCTCGACTACAGCCAGCGTg                             <  4:2124/134‑1 (MQ=255)                                                                                                                                      gagCGACCTGCTGGCCGGGCGCTCGC‑CGGAGCTGCAGATCAACGTCGATGCTACGGCCATGAGCCAAGCGTTCATGGGCGCCGGCTACATCGGCCGCATCTTCGAGCGCGAGCTGCTCGACTACAGCCAGCGTGGTAACGAGCAAAGcc              >  3:205130/1‑149 (MQ=255)                                                                                                                                      gagCGACCTGCTGGCCGGGCGCTCGC‑CGGAGCTGCAGATCAACGTCGATGCTACGGCCATGAGCCAAGCGTTCATGGGCGCCGGCTACATCGGCCGCATCTTCGAGCGCGAGCTGCTCGACTACAGCCAGCGTGGTAACGAGCAAAGcc              >  3:209785/1‑149 (MQ=255)                                                                                                                                      gagCGACCTGCTGGCCGGGCGCTCGC‑CGGAGCTGCAGATCAACGTCGATGCTACGGCCATGAGCCAAGCGTTCATGGGCGCCGGCTACATCGGCCGCATCTTCGAGCGCGAGCTGCTCGACTACAGCCAGCGTGGTAACGAGCAAAGcc              <  5:324928/149‑1 (MQ=255)                                                                                                                                        gCGACCTGCTGGCCGGGCGCTCGC‑CGGAGCTGCAGATCAACGTCGATGCTACGGCCATGAGCCAAGCGTTCATGGGCGCCGGCTACATCGGCCGCATCTTCGAGCGCGAGCTGCTCGACTACAGCCAGCGTGGTAACGAGCAAAGCCCg            <  6:356315/149‑1 (MQ=255)                                                                                                                                                 tgGCCGGGCGCTCGC‑CGGAGCTGCAGATCAACGTCGATGCTACGGCCATGAGCCAAGCGTTCATGGGCGCCGGCTACATCGGCCGCATCTTCGAGCGCGAGCTGCTCGACTACAGCCAGCGTGGTAACGAGCAAAGCCCGGTGCTGAt    >  2:334873/1‑148 (MQ=255)                                                                                                                                                   gCCGGGCGCTCGC‑CGGAGCTGCAGATCAACGTCGATGCTACGGCCATGAGCCAAGCGTTCATTGGCGCCGGCTACATCGGCCGCATCTTCGAGCGCGAGCTGCTCGACTACAGCCAGCGTGGTAACGAGCAAAGCCCGGTGCTGATCa  <  5:194593/148‑1 (MQ=255)                                                                                                                                                     cGGGCGCTCGC‑CGGAGCTGCAGATCAACGTCGATGCTACGGCCATGAGCCAAGCGTTCATGGGCGCCGGCTACATCGGCCGCATCTTCGAGCGCGAGCTGCTCGACTACAGCCAGCGTGGTAACGAGCAAAGCCCGGTGc        <  2:268371/140‑1 (MQ=255)                                                                                                                                                     cGGGCGCTCGC‑CGGAGCTGCAGATCAACGTCGATGCTACGGCCATGAGCCAAGCGTTCATGGGCGCCGGCTACATCGGCCGCATCTTCGAGCGCGAGCTGCTCGACTACAGCCAGCGTGGTAACGAGCAAAGCCCGGTGc        >  1:268371/1‑140 (MQ=255)                                                                                                                                                     |                                                                                                                                                   TCTCGCGCAAGCTCAGCGAAGCCCTGCAACCGCCCGAGTTCCAGCCAGCCGTGCCGCTGCCTCCGCAGCGCCTGGACCAGGCCATGGACAGCGGCCAGTACACCTTCGTCATCAACGTGCCGGTTAACTTCCAGAGCGACCTGCTGGCCGGGCGCTCGC‑CGGAGCTGCAGATCAACGTCGATGCTACGGCCATGAGCCAAGCGTTCATGGGCGCCGGCTACATCGGCCGCATCTTCGAGCGCGAGCTGCTCGACTACAGCCAGCGTGGTAACGAGCAAAGCCCGGTGCTGATCA  >  NC_002947/5940346‑5940639

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑