Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,940,497 G→T 5.4% G112G (GGG→GGT)  yhhJ → translation‑like protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,940,4970GT 5.4% 240.5 / 3.3 74G112G (GGG→GGT) yhhJtranslation‑like protein
Reads supporting (aligned to +/- strand):  ref base G (35/35);  new base T (2/2);  total (37/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.73e-01

GCGCAAGCTCAGCGAAGCCCTGCAACCGCCCGAGTTCCAGCCAGCCGTGCCGCTGCCTCCGCAGCGCCTGGACCAGGCCATGGACAGCGGCCAGTACACCTTCGTCATCAACGTGCCGGTTAACTTCCAGAGCGACCTGCTGGCCGGGCGCTCGC‑CGGAGCTGCAGATCAACGTCGATGCTACGGCCATGAGCCAAGCGTTCATGGGCGCCGGCTACATCGGCCGCATCTTCGAGCGCGAGCTGCTCGACTACAGCCAGCGTGGTAACGAGCAAAGCCCGGTGCTGATCA  >  NC_002947/5940350‑5940639
                                                                                                                                                   |                                                                                                                                               
gcgcAAGCTCAGCGAAGCCCTGCAACCGCCCGAGTTCCAGCCAGCCGTGCCGCTGCCTCCGCAGCGCCTGGACCAGGCCATGGACAGCGGCCAGTACACCTTCGTCATCAACGTGCCGGTTAACTTCCAGAGCGACCTGCTGGCCGGgc                                                                                                                                                <  4:258970/149‑1 (MQ=255)
gcgcAAGCTCAGCGAAGCCCTGCAACCGCCCGAGTTCCAGCCAGCCGTGCCGCTGCCTCCGCAGCGCCTGGACCAGGCCATGGACAGCGGCCAGTACACCTTCGTCATCAACGTGCCGGTTAACTTCCAGAGCGACCTGCCGGCCGGgc                                                                                                                                                <  3:293726/149‑1 (MQ=255)
    aaGCTCAGCGAAGCCCTGCAACCGCCCGAGTTCCAGCCAGCCGTGCCGCTGCCTCCGCAGCGCCTGGACCAGGCCATGGACAGCGGCCAGTACACCTTCGTCATCAACGTGCCGGTTAACTTCCAGAGCGCCCGGCCAGCAGGTCGCTc                                                                                                                                            <  5:126553/149‑1 (MQ=255)
      gCTCAGCGAAGCCCTGCAACCGCCCGAGTTCCAGCCAGCCGTGCCGCTGCCTCCGCAGCGCCTGGACCAGGCCATGGACAGCGGCCAGTACACCTTCGTCATCAACGTGCCGGTTAACTTCCAGAGCGCCCGGCCAGCAGGTCGCTctg                                                                                                                                          <  7:201364/149‑3 (MQ=255)
        tCAGCGAAGCCCTGCAACCGCCCGAGTTCCAGCCAGCCGCGCCGCTGCCTCCGCAGCGCCTGGACCAGGCCATGGACAGCGGCCAGTACACCTTCGTCATCAACGTGCCGGTTAACTTCCAGAGCGACCTGCTGGCCGGGCGCTCGC‑Cg                                                                                                                                       >  4:139352/1‑149 (MQ=255)
          aGCGAAGCCCTGCAACCGCCCGAGTTCCAGCCAGCCGTGCCGCTGCCTCCGCAGCGCCTGGACCAGGCCATGGACAGCGGCCAGTACACCTTCGTCATCAACGTGCCGGTTAACTTCCAGAGCGACCTGCTGGCCGGGCGCTCGC‑Cg                                                                                                                                       >  6:213714/1‑147 (MQ=255)
          aGCGAAGCCCTGCAACCGCCCGAGTTCCAGCCAGCCGTGCCGCTGCCTCCGCAGCGCCTGGACCAGGCCATGGACAGCGGCCAGTACACCTTCGTCATCAACGTGCCGGTTAACTTCCAGAGCGACCTGCTGGCCGGGCGCTCGC‑CGGa                                                                                                                                     <  4:211341/149‑1 (MQ=255)
          aGCGAAGCCCTGCAACCGCCCGAGTTCCAGCCAGCCGTGCCCCTGCCAACGCACCGCCTGGCACACGCCAAGGACAGCGGCCAGTACAACATCGTAATCAACGTGCCGGGTAACTTACAGAGCGACCGGCTGGCCCGGCGCtagacg                                                                                                                                        >  6:250219/1‑142 (MQ=255)
             gAAGCACTGCAACCGCCCGAGTTCCAGCCAGCCGTGCCGCTGCCTGCGCCGCGGCGGGACCAGGCCATGGGCAGCGGGCAGTACACCGTCGGGATGAACGTGCCGGGTGCCTTCCACAGAGCCAGGCTGGCAGGGGGCTCGC‑Cgggggt                                                                                                                                  >  4:205424/1‑145 (MQ=255)
              aaGCCCTGCAACCGCCCGAGTTCCAGCCAGCCGTGCCGCTGCCTCCGCAGCGCCTGGACCAGGCCATGGACAGCGGCCAGTACACCTTCGTCATCAACGTGCCGGTTAACTTCCAGAGCGACCTGCTGGCCGGGCGCTCGC‑CGGAGCTg                                                                                                                                 <  2:72346/149‑1 (MQ=255)
              aaGCCCTGCAACCGCCCGAGTTCCAGCCAGCCGTGCCGCTGCCTCCGCAGCGCCTGGACCAGGCCATGGACAGCGGCCAGTACACCTTCGTCATCAACGTGCCGGTTAACTTCCAGAGCGACCTGCTGGCCGGGCGCTCGC‑CGGAGCTg                                                                                                                                 <  6:303388/149‑1 (MQ=255)
                gCCCTGCAACCGCCCGAGTTCCAGCCAGCCGTGCCGCTGCCTCCGCAGCGCCTGGACCAGGCCATGGACAGCGGCCAGTACACCTTCGTCATCCACGTGCCGGTTAACTTCCAGAGCGACCTGCTGGCCGGGCGCTCGC‑CGGAGCTg                                                                                                                                 >  7:341227/1‑147 (MQ=255)
                gCCCTGCAACCGCCCGAGTTCCAGCCAGCCGTGCCGCTGCCTCCGCAGCGCCTGGACCAGGCCATGGACAGCGGCCAGTACACCTTCGTCATCAACGTGCCGGTTAACTTCCAGAGCGACCTGCTGGCCGGGCGCTCGC‑CGGAGCTGCa                                                                                                                               <  4:335837/149‑1 (MQ=255)
                     gCAACCGCCCGAGTTCCAGCCAGCCGTGCCGCTGCCTCCGCAGCGCCTGGACCAGGCCATGGACAGCGGCCAGTACACCTTCGTCATCAACGTGCCGGTTAACTTCCAGAGCGACCTGCTGGCCGGGCGCTCGC‑CGGAGCTGCAGATc                                                                                                                           >  2:298349/1‑148 (MQ=255)
                      cAACCGCCCGAGTTCCAGCCAGCCGTGCCGCTGCCTCCGCAGCGCCTGGACCAGGCCATGGACAGCGGCCAGTACACCTTCGTCATCAACGTGCCGGTTAACTTCCAGAGCGACCTGCTGGCCGGGCGCTCGC‑CGGAGCTGCAGATc                                                                                                                           <  5:213714/147‑1 (MQ=255)
                              cGAGTTCCAGCCAGCCGTGCCGCTGCCTCCGCAGCGCCTGGACCAGGCCATGGACAGCGGCCAGTACACCTTCGTCATCAACGTGCCGGTTAACTTCCAGAGCGACCTGCTGGCCGGGCGCTCGC‑CGGAGCTGCAGATCAACGTCGATg                                                                                                                 >  7:33742/1‑149 (MQ=255)
                              cGAGTTCCAGCCAGCAGTGCCGCTGCCTCCGCAGCGCCTGGACCAGGCCATGGACAGCGGCCAGTACACCTTCGTCATCAACGTGCCGGTTAACTTCCAGAGCGACCTGCTGGCCGGGCGCTCGC‑CGGAGCTGCAGATCAACGTCGATg                                                                                                                 <  7:156977/149‑1 (MQ=255)
                                        ccagccGTGCCGCTGCCTCCGCAGCGCCTGGACCAGGCCATGGACAGCGGCCAGTACACCTTCGTCATCAACGTGCCGGTTAACTTCCAGAGCGCCCTGCTGGCCGGGCGCTCGC‑CGGAGCTGCAGATCAACGTCGATGCTACGGCCAt                                                                                                       >  7:149743/1‑149 (MQ=255)
                                                 ccGCTGCCTCCGCAGCGCCTGGACCAGGCCATGGACAGCGGCCAGTACACCTTCGTCATCAACGTGCCGGTTAACTTCCAGAGCGACCTGCTGGCCGGGCGCTCGC‑CGGAGCTGCAGATCAACGTCGATGCTACGGCCATGAGCCAAGc                                                                                              >  7:34571/1‑149 (MQ=255)
                                                   gCTGCCTCCGCAGCGCCTGGACCAGGCCATGGACAGCGGCCAGTACACCTTCGTCATCAACGTGCCGGTTAACTTCCAGAGCGACCTGCTGGCCGGGCGCTCGC‑CGGAGCTGCAGATCAACGTCGATGCTACGGCCATGAGCCAAGCGt                                                                                            <  3:139352/149‑1 (MQ=255)
                                                       ccTCCGCAGCGCCTGGACCAGGCCATGGACAGCGGCCAGTACACCTTCGTCATCAACGTGCCGGTTAACTTCCAGAGCGACCTGCTGGCCGGGCGCTCGC‑CGGAGCTGCAGATCAACGTCGATGCTACGGCCATGAGCCAAGCGTTCAt                                                                                        <  3:117587/149‑1 (MQ=255)
                                                       ccTCCGCAGCGCCTGGACCAGGCCATGGACAGCGGCCAGTACACCTTCGTCATCAACGTGCCGGTTAACTTCCAGAGCGACCTGCTGGCCGGGCGCTCGC‑CGGAGCTGCAGATCAACGTCGATGCTACGGCCATGAGCCAAGCGTTCAt                                                                                        >  6:324928/1‑149 (MQ=255)
                                                       ccTCCGCAGCGCCTGGACCAGGCCATGGACAGCGGCCAGTACACCTTCGTCATCAACGTGCCGGTTAACTTCCAGAGCGACCTGCTGGCCGGGCGCTCGC‑CGGAGCTGCAGATCAACGTCGATGCTACGGCCATGAGCCAAGCGTTCAt                                                                                        <  8:33742/149‑1 (MQ=255)
                                                        cTCCGCAGCGCCTGGACCAGGCCATCGACAGCGGCCAGTACCCCTTCGTCATCAACGTGCCGGTTAACTTCCAGAGCGACCTGCTGGCCGGGCGCTCGC‑CGGAGCTGCAGATCAACGTCGATGCTACGGCCATGAGCCAAGCGTTCATg                                                                                       <  2:358061/149‑1 (MQ=255)
                                                         tCCGCAGCGCCTGGACCAGGCCATGGACAGCGGCCAGTACACCTTCGTCATCAACGTGCCGGTTAACTTCCAGAGCGACCTGCTGGCCGGGCGCTCGC‑CGGAGCTGCAGATCAACGTCGATGCTACGGCCATGAGCCAAGCGTTCATgg                                                                                      >  4:285382/1‑149 (MQ=255)
                                                           cGCAGCGCCTGGACCAGGCCATGGACAGCGGCCAGTACACCTTCGTCATCAACGTGCCGGTTAACTTCCAGAGCGACCTGCTGGCCGGGCGCTCGC‑CGGAGCTGCAGATCAACGTCGATGCTACGGCCATGAGCCAAGCGTTCATGGgc                                                                                    >  3:305612/1‑149 (MQ=255)
                                                                cgcCTGGACCAGGCCATGGACAGCGGCCAGTACACCTTCGTCATCAACGTGCCGGTTAACTTCCAGAGCGACCTGCTGGCCGGGCGCTCGC‑CGGAGCTGCAGATCAACGTCGATGCTACGGCCATGAGCCAAGCGTTCATGGGCGCCgg                                                                               <  8:34571/149‑1 (MQ=255)
                                                                  ccTGGACCAGGCCATGGACAGCGGCCAGTACACCTTCGTCATCAACGTGCCGGTTAACTTCCAGAGCGACCTGCTGGCCGGGCGCTCGC‑CGGAGCTGCAGATCAACGTCGATGCTACGGCCATGAGCCAAGCGTTCATGGGCGCCGGc                                                                              >  6:57285/1‑148 (MQ=255)
                                                                  ccTGGACCAGGCCATGGACAGCGGCCAGTACACCTTCGTCATCAACGTGCCGGTTAACTTCCAGAGCGACCTGCTGGCCGGGCGCTCGC‑CGGAGCTGCAGATCAACGTCGATGCTACGGCCATGAGCCAAGCGTTCATGGGCGCCGGCt                                                                             <  5:57285/149‑1 (MQ=255)
                                                                       aCCAGGCCATGGACAGCGGCCAGTACACCTTCGTCATCAACGTGCCGGTTAACTTCCAGAGCGACCTGCTGGCCGGGCGCTCGC‑CGGAGCTGCAGATCAACGTCGATGCTACGGCCATGAGCCAAGCGTTCATGGGCGCCGGCTACATc                                                                        >  4:100266/1‑149 (MQ=255)
                                                                         cAGGCCATGGACAGCGGCCAGTACACCTTCGTCATCAACGTGCCGGTTAACTTCCAGAGCGACCTGCTGGCCGGGCGCTCGC‑CGGAGCTGCAGATCAACGTCGATGCTACGGCCATGAGCCAAGCGTTCATGGGCGCCGGCtctccaag                                                                      >  6:235890/1‑142 (MQ=255)
                                                                           ggCCATGGACAGCGGCCAGTACACCTTCGTCATCAACGTGCCGGTTAACTTCCAGAGCGACCTGCTGGCCGGGCGCTCGC‑CGGAGCTGCAGATCAACGTCGATGCTACGGCCATGAGCCAAGCGTTCATGGGCGCCGGCTACATCGGcc                                                                    <  3:100266/149‑1 (MQ=255)
                                                                            gCCATGGACAGCGGCCAGTACACCTTCGTCATCAACGTGCCGGTTAACTTCCAGAGCGACCTGCTGGCCGGGCGCTCGC‑CGGAGCTGCAGATCAACGTCGATGCTACGGCCATGAGCCAAGCGTTCATg                                                                                       >  5:266981/1‑129 (MQ=255)
                                                                            gCCATGGACAGCGGCCAGTACACCTTCGTCATCAACGTGCCGGTTAACTTCCAGAGCGACCTGCTGGCCGGGCGCTCGC‑CGGAGCTGCAGATCAACGTCGATGCTACGGCCATGAGCCAAGCGTTCATg                                                                                       <  6:266981/129‑1 (MQ=255)
                                                                             ccATGGACAGCGGCCAGTACACCTTCGTCATCAACGTGCCGGTTAACTTCCAGAGCGACCTGCTGGCCGGGCGCTCGC‑CGGAGCTGCAGATCAACGTCGATGCTACGGCCATGAGCCAAGCGTTCATGGGCGCCGGCTACATCGGCCg                                                                   >  1:50023/1‑148 (MQ=255)
                                                                              cATGGACAGCGGCCAGTACACCTTCGTCATCAACGTGCCGGTTAACTTCCAGAGCGACCTGCTGGCCGGGCGCTCGC‑CGGAGCTGCAGATCAACGTCGATGCTACGGCCATGAGCCAAGCGTTCATGGGCGCCGGCTACATCGGCCGCa                                                                 >  4:133368/1‑149 (MQ=255)
                                                                              cATGGACAGCGGCCAGTACACCTTCGTCATCAACGTGCCGGTTAACTTCCAGAGCGACCTGCTGGCCGGGCGCTCGC‑CGGAGCTGCAGATCAACGTCGATGCTACGGCCATGAGCCAAGCGTTCATGGGCGCCGGCTACATCGGCCGCa                                                                 >  5:125376/1‑149 (MQ=255)
                                                                                    cAGCGGCCAGTACACCTTCGTCATCAACGTGCCGGTTAACTTCCAGAGCGACCTGCTGGCCGGGCGCTCGC‑CGGAGCTGCAGATCAACGTCGATGCTACGGCCATGAGCCAAGCGTTCATGGGCGCCGGCTACATCGGCCGCATCTTCg                                                           <  3:285382/149‑1 (MQ=255)
                                                                                     aGCGGCCAGTACACCTTCGTCATCAACGTGCCGGTTAACTTCCAGAGCGACCTGCTGGCCGGGCGCTCGC‑CGGAGCTGCAGATCAACGTCGATGCTACGGCCATGAGCCAAGCGTTCATGGGCGCCGGCTACATCGGCCGCATCTTCGa                                                          <  3:98629/149‑1 (MQ=255)
                                                                                     aGCGGCCAGTACACCTTCGTCATCAACGTGCCGGTTAACTTCCAGAGCGACCTGCTGGCCGGGCGCTCGC‑CGGAGCTGCAGATCAACGTCGATGCTACGGCCATGAGCCAAGCGTTCATGGGCGCCGGCTACATCGGCCGCATCTTCGa                                                          <  8:149743/149‑1 (MQ=255)
                                                                                              tACACCTTCGTCATCAACGTGCCGGTTACCTTCCAGAGCGAGCTGCTGGCCGGGCGCTCGC‑CGGAGCTGCAGATCAACGTCGATGCTACGGCCATGAGCGAAGCGTTCATGGGCGCCGGCTACATCGTCCGCATCTTCGAGCGCGAgc                                                  <  5:323421/148‑1 (MQ=255)
                                                                                              tACACCTTCGTCATCAACGTGCCGGTTAACTTCCAGAGCGACCTGCTGGCCGGGCGCTCGC‑CGGAGCTGCAGATCAACGTCGATGCTACGGCCATGAGCCAAGCGTTCATGGGCGCCGGCTACATCGGCCGCATCTTCGAGCGCGAgct                                                 >  8:236963/1‑149 (MQ=255)
                                                                                              tACACCTTCGTCATCAACGTGCCGGTTAACTTCCAGAGCGACCTGCTGGCCGGGCGCTCGC‑CGGAGCTGCAGATCAACGTCGATGCTACGGCCATGAGCCAAGCGTTCATGGGCGCCGGCTACATCGGCCGCATCTTCCAGCGCGAgc                                                  >  6:323421/1‑148 (MQ=255)
                                                                                                cacCTTCGTCATCAACGTGCCGGTTAACTTCCAGAGCGACCTGCTGGCCGGGCGCTCGC‑CGGAGCTGCAGATCAACGTCGATGCTACGGCCATGAGCCAAGCGTTCATGGGCGCCGGCTACATCGGCCGCATCTTCGAGCGCGAgctgc                                               <  6:224586/149‑1 (MQ=255)
                                                                                                  ccTTCGTCATCACCGTGCCGGTTAACTTCCAGAGCGACCTGCTGGCCGGGCGCTCGC‑CGGAGCTGCAGATCAACGTCGATGCTACGGCCATGAGCCAAGCGTTCATGGGCGCCGGCTACATCGGCCGCATCTTCGAGCGCGAGCTGCTc                                             <  7:205628/149‑1 (MQ=255)
                                                                                                  ccTTCGTCATCAACGTGCCGGTTAACTTCCAGAGCGACCTGCTGGCCGGGCGCTCGC‑CGGAGCTGCAGATCAACGTCGATGCTACGGCCATGAGCCAAGCGTTCATGGGCGCCGGCTACATCGGCCGCATCTTCGAGCGCGAGCTGCTc                                             <  8:341227/149‑1 (MQ=255)
                                                                                                   cTTCGTCATCAACGTGCCGGTTAACTTCCAGAGCGACCTGCTGGCCGGGCGCTCGC‑CGGAGCTGCAGATCAACGTCGATGCTACGGCCATGAGCCAAGCGTTCATGGGCGCCGGCTACATCGGCCGCATCTTCGAGCGCGAGCTGCTc                                             >  8:270266/1‑148 (MQ=255)
                                                                                                   cTTCGTCATCAACGTGCCGGTTAACTTCCAGAGCGACCTGCTGGCCGGGCGCTCGC‑CGGAGCTGCAGATCAACGTCGATGCTACGGCCATGAGCCAAGCGTTCATGGGCGCCGGCTACATCGGCCGCATCTTCGAGCGCGAGCTGCTc                                             >  3:129659/1‑148 (MQ=255)
                                                                                                      cGTCATCAACGTGCCGGTTAACTTCCAGAGCGACCTGCTGGCCGGGCGCTCGC‑CGGAGCTGCAGATCAACGTCGATGCTACGGCCATGAGCCAAGCGTTCATGGGCGCCGGCTACATCGGCCGCATCTTCGAGCGCGAGCTGCTCGAc                                          <  7:236963/148‑1 (MQ=255)
                                                                                                        tcatcaACGTGCCGGTTAACTTCCAGAGCGACCTGCTGGCCGGGCGCTCGC‑CGGAGCTGCAGATCAACGTCGATGCTACGGCCATGAGCCAAGCGTTCATGGGCGCCGGCTACATCGGCCGCATCTTCGAGCGCGAGCTGCTCGACTAc                                       <  2:50023/149‑1 (MQ=255)
                                                                                                          atcaACGTGCCGGTTAACTTCCAGAGCGACCTGCTGGCCGGGCGCTCGCGCGATTGTGCAGATCTTCGTCGATGCTACGGCCATGAGCCAAGCGTTCATGGGCGCCGGCTACATCGGCCGCATCTTCGAGCGCGAGCTGCTCGACTAca                                      <  1:298349/149‑1 (MQ=255)
                                                                                                          atcaACGTGCCGGTTAACTTCCAGAGCGACCTGCTGGCCGGGCGCTCGC‑CGGAGCTGCAGATCAACGTCGATGCTACGGCCATGAGCCAAGCGTTCATGGGCGCCGGCTACATCGGCCGCATCTTCGAGCGCGAGCTGCTc                                             <  8:182053/141‑1 (MQ=255)
                                                                                                          atcaACGTGCCGGTTAACTTCCAGAGCGACCTGCTGGCCGGGCGCTCGC‑CGGAGCTGCAGATCAACGTCGATGCTACGGCCATGAGCCAAGCGTTCATGGGCGCCGGCTACATCGGCCGCATCTTCGAGCGCGAGCTGCTCGACTAcag                                     >  5:356315/1‑149 (MQ=255)
                                                                                                          atcaACGTGCCGGTTAACTTCCAGAGCGACCTGCTGGCCGGGCGCTCGC‑CGGAGCTGCAGATCAACGTCGATGCTACGGCCATGAGCCAAGCGTTCATGGGCGCCGGCTACATCGGCCGCATCTTCGAGCGCGAGCTGCTCGACTAcag                                     >  3:360048/1‑149 (MQ=255)
                                                                                                          atcaACGTGCCGGTTAACTTCCAGAGCGACCTGCTGGCCGGGCGCTCGC‑CGGAGCTGCAGATCAACGTCGATGCTACGGCCATGAGCCAAGCGTTCATGGGCGCCGGCTACATCGGCCGCATCTTCGAGCGCGAGCTGCTCGACTAca                                      <  3:133368/148‑1 (MQ=255)
                                                                                                          atcaACGTGCCGGTTAACTTCCAGAGCGACCTGCTGGCCGGGCGCTCGC‑CGGAGCTGCAGATCAACGTCGATGCTACGGCCATGAGCCAAGCGTTCATGGGCGACGGCTACATCGGCCGCATCTTCGAGCGCGAGCTGCTCGagatag                                      >  7:182053/1‑143 (MQ=255)
                                                                                                             aaCGTGCCGGTTAACTTCCAGAGCGACCTGCTGGCCGGGCGCTCGC‑CGGAGCTGCAGATCAACGTCGATGCTACGGCCATGAGCCAAGCGTTCATGGGCGCCGGCTACATCGGCCGCATCTTCGAGCGCGAGCTGCTCGACTAcagcca                                  <  4:305612/149‑1 (MQ=255)
                                                                                                                  gCCGGTTAACTTCCAGAGCGACCTGCTGGCCGGGCGCTCGC‑CGGAGCTGCAGATCAACGTCGATGCTACGGCCATGAGCCAAGCGTTCATGGGCGCCGGCTACATCGGCCGCATCTTCGAGCGCGAGCTGCTCGACTACAGCCAGCGTg                             >  4:356950/1‑149 (MQ=255)
                                                                                                                      gTTAACTTCCAGGGCGACCTGCTGGCCGGGCGCTCGC‑CGGAGCTGCAGATCAACGTCGATGCTACGGCCATGAGCCAAGCGTTCATGGGCGCCGGCTACATCGGCCGCATCTTCGAGCGCGAGCTGCTCGACTACAGCCAGCGTGGTaa                         >  8:235904/1‑149 (MQ=255)
                                                                                                                         aaCTTCCAGAGCGACCTGCTGGCCGGGCGCTCGC‑CGGAGCTGCAGATCAACGTCGATGCTACGGCCATGAGCCAAGCGTTCATGGGCGCCGGCTACATCGGCCGCATCTTCGAGCGCGAGCTGCTCGACTACAGCCAGCGTGGTAAc                        >  6:55452/1‑147 (MQ=255)
                                                                                                                           cTTCCAGAGCGACCTGCTGGCCGGGCGCTCGC‑CGGAGCTGCAGATCAACGTCGATGCTACGGCCATGAGCCAAGCGTTCATGGGCGCCGGCTACATCGGCCGCATCTTCGAGCGCGAGCTGCTCGACTACAGCCAGCGTGGTAACGAGc                    <  6:125376/149‑1 (MQ=255)
                                                                                                                           cTTCCAGAGCGACCTGCTGGCCGGGCGCTCGC‑CGGAGCTGCAGATCAACGTCGATGCTACGGCCATGAGCCAAGCGTTCATGGGCGCCGGCTACATCGGCCGCATCTTCGAGCGCGAGCTGCTCGACTACAGCCAGCGTGGTAACGAGc                    <  7:270266/149‑1 (MQ=255)
                                                                                                                            ttCCAGAGCGACCTGCTGGCCGGGCGCTCGC‑CGGAGCTGCAGATCAACGTCGATGCTACGGCCATGAGCCAAGCGTTCATGGGCGCCGGCTACATCGGCCGCATCTTCGAGCGCGAGCTGCTCGACTACAGCCAGCGTGGTAACGAGc                    >  6:194593/1‑148 (MQ=255)
                                                                                                                             cggcgagCGCCCGGCCAGCAGGTCGCTCGC‑CGGAGCTGCAGATCAACGTCGATGCTACGGCCATGAGCCAAGCGTTCATGGGCGCCGGCTACATCGGCCGCATCTTCGAGCGCGAGCTGCTCGACTACAGCCAGCGTGGTAACGAGCa                   >  2:166226/5‑148 (MQ=255)
                                                                                                                               gcgagCGCCCGGCCAGCAGGTCGCTCGC‑CGGAGCTGCAGATCAACGTCGATGCTACGGCCATGAGCCAAGCGTTCATGGGCGCCGGCTACATCGGCCGCATCTTCGAGCGCGAGCTGCTCGACTACAGCCAGCGTGGTAACGAGCAAAg                >  7:287907/3‑149 (MQ=255)
                                                                                                                               cAGAGCGACCTGCTGGCCGGGCGCTCGC‑CGGAGCTGCAGATCAACGTCGATGCTACGGCCATGAGCCAAGCGTTCATGGGCGCCGGCTACATCGGCCGCATCTTCGAGCGCGAGCTGCTCGACTACAGCCAGCGTGGTAACGAGCaaa                 <  5:250219/148‑1 (MQ=255)
                                                                                                                                 gagCGACCTGCTGGCCGGGCGCTCGC‑CGGAGCTGCAGATCAACGTCGATGCTACGGCCATGAGCCAAGCGTTCATGGGCGCCGGCTACATCGGCCGCATCTTCGAGCGCGAGCTGCTCGACTACAGCCAGCGTg                             <  4:2124/134‑1 (MQ=255)
                                                                                                                                 gagCGACCTGCTGGCCGGGCGCTCGC‑CGGAGCTGCAGATCAACGTCGATGCTACGGCCATGAGCCAAGCGTTCATGGGCGCCGGCTACATCGGCCGCATCTTCGAGCGCGAGCTGCTCGACTACAGCCAGCGTg                             >  3:2124/1‑134 (MQ=255)
                                                                                                                                 gagCGACCTGCTGGCCGGGCGCTCGC‑CGGAGCTGCAGATCAACGTCGATGCTACGGCCATGAGCCAAGCGTTCATGGGCGCCGGCTACATCGGCCGCATCTTCGAGCGCGAGCTGCTCGACTACAGCCAGCGTGGTAACGAGCAAAGcc              >  3:209785/1‑149 (MQ=255)
                                                                                                                                 gagCGACCTGCTGGCCGGGCGCTCGC‑CGGAGCTGCAGATCAACGTCGATGCTACGGCCATGAGCCAAGCGTTCATGGGCGCCGGCTACATCGGCCGCATCTTCGAGCGCGAGCTGCTCGACTACAGCCAGCGTGGTAACGAGCAAAGcc              >  3:205130/1‑149 (MQ=255)
                                                                                                                                 gagCGACCTGCTGGCCGGGCGCTCGC‑CGGAGCTGCAGATCAACGTCGATGCTACGGCCATGAGCCAAGCGTTCATGGGCGCCGGCTACATCGGCCGCATCTTCGAGCGCGAGCTGCTCGACTACAGCCAGCGTGGTAACGAGCAAAGcc              <  5:324928/149‑1 (MQ=255)
                                                                                                                                   gCGACCTGCTGGCCGGGCGCTCGC‑CGGAGCTGCAGATCAACGTCGATGCTACGGCCATGAGCCAAGCGTTCATGGGCGCCGGCTACATCGGCCGCATCTTCGAGCGCGAGCTGCTCGACTACAGCCAGCGTGGTAACGAGCAAAGCCCg            <  6:356315/149‑1 (MQ=255)
                                                                                                                                            tgGCCGGGCGCTCGC‑CGGAGCTGCAGATCAACGTCGATGCTACGGCCATGAGCCAAGCGTTCATGGGCGCCGGCTACATCGGCCGCATCTTCGAGCGCGAGCTGCTCGACTACAGCCAGCGTGGTAACGAGCAAAGCCCGGTGCTGAt    >  2:334873/1‑148 (MQ=255)
                                                                                                                                              gCCGGGCGCTCGC‑CGGAGCTGCAGATCAACGTCGATGCTACGGCCATGAGCCAAGCGTTCATTGGCGCCGGCTACATCGGCCGCATCTTCGAGCGCGAGCTGCTCGACTACAGCCAGCGTGGTAACGAGCAAAGCCCGGTGCTGATCa  <  5:194593/148‑1 (MQ=255)
                                                                                                                                                cGGGCGCTCGC‑CGGAGCTGCAGATCAACGTCGATGCTACGGCCATGAGCCAAGCGTTCATGGGCGCCGGCTACATCGGCCGCATCTTCGAGCGCGAGCTGCTCGACTACAGCCAGCGTGGTAACGAGCAAAGCCCGGTGc        <  2:268371/140‑1 (MQ=255)
                                                                                                                                                cGGGCGCTCGC‑CGGAGCTGCAGATCAACGTCGATGCTACGGCCATGAGCCAAGCGTTCATGGGCGCCGGCTACATCGGCCGCATCTTCGAGCGCGAGCTGCTCGACTACAGCCAGCGTGGTAACGAGCAAAGCCCGGTGc        >  1:268371/1‑140 (MQ=255)
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GCGCAAGCTCAGCGAAGCCCTGCAACCGCCCGAGTTCCAGCCAGCCGTGCCGCTGCCTCCGCAGCGCCTGGACCAGGCCATGGACAGCGGCCAGTACACCTTCGTCATCAACGTGCCGGTTAACTTCCAGAGCGACCTGCTGGCCGGGCGCTCGC‑CGGAGCTGCAGATCAACGTCGATGCTACGGCCATGAGCCAAGCGTTCATGGGCGCCGGCTACATCGGCCGCATCTTCGAGCGCGAGCTGCTCGACTACAGCCAGCGTGGTAACGAGCAAAGCCCGGTGCTGATCA  >  NC_002947/5940350‑5940639

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑