Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,962,276 G→T 5.3% G391V (GGC→GTC)  lysA‑II → diaminopimelate decarboxylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,962,2760GT 5.3% 243.3 / 3.3 75G391V (GGC→GTC) lysA‑IIdiaminopimelate decarboxylase
Reads supporting (aligned to +/- strand):  ref base G (31/40);  new base T (2/2);  total (33/42)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.34e-01

GCGAGACTGGCGACTTCCTCGGCAAGGACCGCGTCCTCAACCTGGCCGAAGGTGACCTGCTGGCCGTAGAGTCCGCGGGCGCCTATGGTTTTGTCATGAGCTCCAACTACAACACCCGCGGCCGTTGCGCTGAAATCCTGGTCGACGGCGACCAGGCGTTCGAAGTACGCCGCCGCGAGACCGTCGCCGAACTGTATGCTGGCGAAAGCCTGCTGCCGGAGTAAGCCCATGCTGCTGCGTTTTACCAAGATGCATGGGCTGGGCAACGACTTCATGGTCCTGGACCTGGTCAGCC  >  NC_002947/5962129‑5962423
                                                                                                                                                   |                                                                                                                                                   
gCGAGACTGGCGACTTCCTCGGCAAGGACCGCGTCCTCAACCTGGCCGAAGGTGACCTGCTGGCCGTAGAGTCCGCGGGCGCCTATGGTTTTGTCATGAGCTCCAACTACAACACCCGCGGCCGTTGCGCTGAAATCCTGGTCGACGGc                                                                                                                                                    >  4:106712/1‑149 (MQ=255)
 cGAGACTGGCGACTTCCTCGGCAAGGACCGCGTCCTCAACCTGGCCGAAGGTGACCTGCTGGCCGTAGAGTCCGCGGGCGCCTATGGTTTTGTCATGAGCTCCAACTACAACACCCGCGGCCGTTGCGCTGAAATCCTGGTCGACGGc                                                                                                                                                    <  4:21914/148‑1 (MQ=255)
  gagaCTGGCGACTTCCTCGGCAAGGACCGCGTCCTCAACCTGGCCGAAGGTGACCTGCTGGCCGTAGAGTCCGCGGGCGCCTATGGTTTTGTCATGAGCTCCAACTACAACACCCGCGGCCGTTGCGCTGAAATCCTGGTCGACGGCGa                                                                                                                                                  >  3:198930/1‑149 (MQ=255)
        ggCGACTTCCTCGGCAAGGACCGCGTCCTCAACCTGGCCGAAGGTGACCTGCTGGCCGTAGAGTCCGCGGGCGCCTATGGTTTTGTCATGAGCTCCAACTACAACACCCGCGGCCGTTGCGCTGAAATCCTGGTCGACGGCGACCAGGc                                                                                                                                            >  5:17473/1‑149 (MQ=255)
        ggCGACTTCCTCGGCAAGGACCGCGTCCTCAACCTGGCCGAAGGTGACCTGCTGGCCGTAGAGTCCGCGGGCGCCTATGGTTTTGTCATGAGCTCCAACTACAACACCCGCGGCCGTTGCGCTGAAATCCTGGTCGACGGCGACCAGGc                                                                                                                                            >  1:77913/1‑149 (MQ=255)
         gCGACTTCCTCGGCAAGGACCGCGTCCTCAACCTGGCCGAAGGTGACCTGCTGGCCGTAGAGTCCGCGGGCGCCTATGGTTTTGTCATGAGCTCCAACTACAACACCCGCGGCCGTTGCGCTGAAATCCTGGTCGCCGTCGACCAggat                                                                                                                                           <  1:76265/149‑3 (MQ=255)
           gACTTCCTCGGCAAGGACCGCGTCCTCAACCTGGCCGAAGGTGACCTGCTGGCCGTAGAGTCCGCGGGCGCCTATGGTTTTGTCATGAGCTCCAACTACAACACCCGCGGCCGTTGCGCTGAAATCCTGGTCGACGGCGACCAGGCGtt                                                                                                                                         <  8:42671/149‑1 (MQ=255)
                ccTCGGCAAGGACCGCGTCCTCAACCTGGCCGAAGGTGACCTGCTGGCCGTAGAGTCCGCGGGCGCCTATGGTTTTGTCATGAGCTCCAACTACAACACCCGCGGCCGTTGCGCTGAAATCCTGGTCGACGGCGACCAGGCGTTCGaa                                                                                                                                     <  5:314100/148‑1 (MQ=255)
                    ggCAAGGACCGCGTCCTCAACCTGGCCGAAGGTGACCTGCTGGCCGTAGAGTCCGCGGGCGCCTATGGTTTTGTCATGAGCTCCAACTACAACACCCGCGGCCGTTGCGCTGAAATCCTGGTCGACGGCGACCAGGCGTTCGAAGTAc                                                                                                                                 <  4:129678/148‑1 (MQ=255)
                    ggCAAGGACCGCGTCCTCAACCTGGCCGAAGGTGACCTGCTGGCCGTAGAGTCCGCGGGCGCCTATGGTTTTGTCATGAGCTCCAACTACAACACCCGCGGCCGTTGCGCTGAAATCCTGCTCGACGGCGACCAGGCGTTCGAAGTAcg                                                                                                                                <  1:201959/149‑1 (MQ=255)
                     gCAAGGACCGCGTCCTCAACCTGGCCGAAGGTGACCTGCTGGCCGTAGAGTCCGCGGGCGCCTATGGTTTTGTCATGAGCTCCAACTACAACACCCGCGGCCGTTGCGCTGAAATCCTGGTCGACGGCGACCAGGCGTTCGAAGTAcgc                                                                                                                               >  5:85688/1‑149 (MQ=255)
                      cAAGGACCGCGTCCTCAACCTGGCCGAAGGTGACCTGCTGGCCGTAGAGTCCGCGGGCGCCTATGGTTTTGTCATGAGCTCCAACTACAACACCCGCGGCCGTTGCGCTGAAATCCTGGTCGACGGCGACCAGGCGTTCGAAGTAcgcc                                                                                                                              <  1:327431/149‑1 (MQ=255)
                       aaGGACCGCGTCCTCAACCTGGCCGAAGGTGACCTGCTGGCCGTAGAGTCCGCGGGCGCCTATGGTTTTGTCATGAGCTCCAACTACAACACCCGCGGCCGTTGCGCTGAAATCCTGGTCGACGGCGACCAGGCGTTCGaa                                                                                                                                     >  6:223991/1‑141 (MQ=255)
                       aaGCACCGCGTCCTCAACCTGGCCGAAGGTGACCTGCTGGCCGTAGAGTCCGCGGCCGCCTATGGTTTTGTCATGAGCTCCAACTACAACACCCGCGGCCGTTGCGCTGAAATCCTGGTCGACGGCGACCAGGCGTTCGaa                                                                                                                                     <  5:223991/141‑1 (MQ=255)
                             cgcgTCCTCAACCTGGCCGAAGGTGACCTGCTGGCCGTAGAGTCCGCGGGCGCCTATGGTTTTGTCATGAGCTCCAACTACAACACCCGCGGCCGTTGCGCTGAAATCCTGGTCGACGGCGACCAGGCGTTCGAAGTACGCCGCCGCga                                                                                                                       <  7:138622/149‑1 (MQ=255)
                             cgcgTCCTCAACCTGGCCGAAGGTGACCTGCTGGCCGTAGAGTCCGCGGGCGCCTATGGTTTTGTCATGAGCTCCAACTACAACACCCGCGGCCGTTGCGCTGAAATCCTGGTCGACGGCGACCAGGCGTTCGAAGTACGCCGCCGCga                                                                                                                       <  2:274871/149‑1 (MQ=255)
                               cgTCCTCAACCTGGCCGAAGGTGACCTGCTGGCCGTAGAGTCCGCGGGCGCCTATGGTTTTGTCATGAGCTCCAACTACAACACCCGCGGCCGTTGCGCTGAAATCCTGGTCGACGGCGACCAGGCGTTCGAAGTACGCCGCCGCgaga                                                                                                                     <  3:106712/149‑1 (MQ=255)
                               cgTCCTCAACCTGGCCGAAGGTGACCTGCTGGCCGTAGAGTCCGCGGGCGCCTATGGTTTTGTCATGAGCTCCAACTACAACACCCGCGGCCGTTGCGCTGAAATCCTGGTCGACGGCGACCAGGCGTTCGAAGTACGCCGCCGCgaga                                                                                                                     >  2:222430/1‑149 (MQ=255)
                                   cTCAACCTGGCCGAAGGTGACCTGCTGGCCGTAGAGTCCGCGGGCGCCTATGGTTTTGTCATGAGCTCCAACTACAACACCCGCGGCCGTTGCGCTGAAATCCTGGTCGACGGCGACCAGGCGTTCGAAGTACGCCGCCGCGAGACCGt                                                                                                                 >  4:250018/1‑149 (MQ=255)
                                            gCCGAAGGTGACCTGCTGGCCGTAGAGTCCGCGGGCGCCTATGGTTTTGTCATGAGCTCCAACTACAACACCCGCGGCCGTTGCGCTGAAATCCTGGTCGACGGCGACCAGGCGTTCGAAGTACGCCGCCGCGAGACCGTCGCCGaa                                                                                                          <  3:250018/147‑1 (MQ=255)
                                            gCCGAAGGTGACCTGCTGGCCGTAGAGTCCGCGGGCGCCTATGGTTTTGTCATGAGCTCCAACTACAACACCCGCGGCCGTTGCGCTGAAATCCTGGTCGACGGCGACCAGGCGTTCGAAGTACGCCGCCGCGAGACCGTCGCCGAAc                                                                                                         <  1:222430/148‑1 (MQ=255)
                                              cGAAGGTGACCTGCTGGCCGTAGAGTCCGCGGGCGCCTATGGTTTTGTCATGAGCTCCAACTACAACACCCGCGGCCGTTGCGCTGAAATCCTGGTCGACGGCGACCAGGCGTTCGAAGTACGCCGCCGCGAGACCGTCGCCGAACTg                                                                                                       >  6:191618/1‑148 (MQ=255)
                                                aaGGTGACCTGCTGGCCGTAGAGTCCGCGGGCGCCTATGGTTTTGTCATGAGCTCCAACTACAACACCCGCGGCCGTTGCGCTGAAATCCTGGTCGACGGCGACCAGGCGTTCGAAGTACGCCGCCGCGAGACCGTCGCCGAACTGTAt                                                                                                    <  6:85688/149‑1 (MQ=255)
                                                          gctgGCCGTAGAGTCCGCGGGCGCCTATGGTTTTGTCATGAGCTCCAACTACAACACCCGCGGCCGTTGCGCTGAAATCCTGGTCGACGGCGACCAGGCGTTCGAAGTACGCCGCCGCGAGACCGTCGCCGAACTGTATGCTGGCGa                                                                                            >  1:119253/1‑147 (MQ=255)
                                                           ctgGCCGTAGAGTCCGCGGGCGCCTATGGTTTTGTCATGAGCTCCAACTACAACACCCGCGGCCGTTGCGCTGAAATCCTGGTCGACGGCGACCAGGCGTTCGAAGTACGCCGCCGCGAGACCGTCGCCGAACTGTATGCTGGCGAAAg                                                                                         <  1:60667/149‑1 (MQ=255)
                                                           ctgGCCGTAGAGTCCGCGGGCGCCAATGGTTTTGTCATGAGCTCCAACTACAACACCCGCGGCCGTTGCGCTGAAATCCTGGTCGACGGCGACCAGGCGTTCGAAGTACGCCCCCGCCAGACCGTAGCCGAACTGTATGCTGGCGAAAg                                                                                         >  4:327839/1‑149 (MQ=255)
                                                              gCCGTAGAGTCCGCGGGCGCCTATGGTTTTGTCATGAGCTCCAACTACAACACCCGCGGCCGTTGCGCTGAAATCCTGGTCGACGGCGACCAGGCGTTCGAAGTACGCCGCCGCGAGACCGTCGCCGAACTGTATGCTGGCGAAAGc                                                                                        <  3:47942/147‑1 (MQ=255)
                                                                 gTAGAGTCCGCGGGCGCCTATGGTTTTGTCATGAGCTCCAACTACAACACCCGCGGCCGTTGCGCTGAAATCCTGGTCGACGGCGACCAGGCGTTCGAAGTACGCCGCCGCGAGACCGTCGCCGAACTGTATGCTGGCGAAAGCctgct                                                                                   <  7:288541/149‑1 (MQ=255)
                                                                       tCCGCGGGCGCCTATGGTTTTGTCATGAGCTCCAACTACAACACCCGCGGCCGTTGCGCTGAAATCCTGGTCGACGGCGACCAGGCGTTCGAAGTACGCCGCCGCGAGACCGTCGCCGAACTGTATGCTGGCGAAAGCCTGCTGCCg                                                                               >  7:215557/1‑147 (MQ=255)
                                                                       tCCGCGGGCGCCTATGGTTTTGTCATGAGCTCCAACTACAACACCCGCGGCCGTTGCGCTGAAATCCTGGTCGACGGCGACCAGGCGTTCGAAGTACGCCGCCGCGAGACCGTCGCCGAACTGTATGCTGGCGAAAGCCTGCTGCCGGa                                                                             <  3:312802/149‑1 (MQ=255)
                                                                       tCCGCGGGCGCCTATGGTTTTGTCATGAGCTCCAACTACAACACCCGCGGCCGTTGCGCTGAAATCCTGGTCGACGGCGACCAGGCGTTCGAAGTACGCCGCCGCGAGACCGTCGCCGAACTGTATGCTGGCGAAAGCCTGCTGCCGGa                                                                             <  8:215557/149‑1 (MQ=255)
                                                                          gcgGGCGCCTATGGTTTTGTCATGAGCTCCAACTACAACACCCGCGGCCGTTGCGCTGAAATCCTGGTCGACGGCGACCAGGCGTTCGAAGTACGCCGCCGCGAGACCGTCGCCGAACTGTATGCTGGCGAAAGCCTGCTGCCGGAGt                                                                           >  1:357510/1‑148 (MQ=255)
                                                                              gcgcCTATGGTTTTGTCATGAGCTCCAACTACAACACCCGCGGCCGTTGCGCTGAAATCCTGGTCGACGGCGACCAGGCGTTCGAAGTACGCCGCCGCGAGACCGTCGCCGAACTGTATGCTGGCGAAAGCCTGCTGCCGGAGTAAGcc                                                                      <  5:191618/149‑1 (MQ=255)
                                                                                 ccTATGGTTTTGTCATGAGCTCCAACTACAACACCCGCGGCCGTTGCGCTGAAATCCTGGTCGACGGCGACCAGGCGTTCGAAGTACGCCGCCGCGAGACCGTCGCCGAACTGTATGCTGGCGAAAGCCTGCTGCCGGAGTAAGCCCAt                                                                   <  4:198930/149‑1 (MQ=255)
                                                                                   tATGGTTTTGTCATGAGCTCCAACTACAACACCCGCGGCCGTTGCGCTGAAATCCTGGTCGACGGCGACCAGGCGTTCGAAGTACGCCGCCGCGAGACCGTCGCCGAACTGTATGCTGGCGAAAGCCTGCTGCCGGAGTAAGCCCAtgc                                                                 >  7:198270/1‑149 (MQ=255)
                                                                                   tATGGTTTTGTCATGAGCTCCAACTACAACACCCGCGGCCGTTGCGCTGAAATCCTGGTCGACGGCGACCAGGCGTTCGAAGTACGCCGCCGCGAGACCGTCGCCGAACTGTATGCTGGCGAAAGCCTGCTGCCGGAGTAAGCCCAtgc                                                                 >  7:290886/1‑149 (MQ=255)
                                                                                    aTGGTTTTGTCATGAGCTCCAACTACAACACCCGCGGCCGTTGCGCTGAAATCCTGGTCGACGGCGACCAGGCGTTCGAAGTACGCCGCCGCGAGACCGTCGCCGAACTGTATGCTGGCGAAAGCCTGCTGCCGGAGTAAGCCCa                                                                    >  4:46362/1‑145 (MQ=255)
                                                                                    aTGGTTTTGTCATGAGCTCCAACTACAACACCCGCGGCCGTTGCGCTGAAATCCTGGTCGACGGCGACCAGGCGTTCGAAGTACGCCGCCGCGAGACCGTCGCCGAACTGTATGCTGGCGAAAGCCTGCTGCCGGAGTAAGCCCa                                                                    <  3:46362/145‑1 (MQ=255)
                                                                                       gTTTTGTCATGAGCTCCAACTACAACACCCGCGGCCGTTGCGCTGAAATCCTGGTCGACGGCGACCAGGCGTTCGAAGTACGCCGCCGCGAGACCGTCGCCGAACTGTATGCTGGCGAAAGCCTGCTGCCGGAGTAAGCCCAtgctgct                                                             >  7:186489/1‑149 (MQ=255)
                                                                                        ttttGTCATGAGCTCCAACTACAACACCCGCGGCCGTTGCGCTGAAATCCTGGTCGACGGCGACCAGGCGTTCGAAGTACGCCGCCGCGAGACCGTCGCCGAACTGTATGCTGGCGAAAGCCTGCTGCCGGAGTAAGCCCAtgctgctg                                                            >  7:340558/1‑149 (MQ=255)
                                                                                               aTGAGCTCCAACTACAACACCCGCGGCCGTTGCGCTGAAATCCTGGTCGACGGCGACCAGGCGTTCGAAGTACGCCGCCGCGAGACCGTCGCCGAACTGTATGCTGGCGAAAGCCTGCTGCCGGAGt                                                                           >  6:302081/1‑127 (MQ=255)
                                                                                               aTGAGCTCCAACTACAACACCCGCGGCCGTTGCGCTGAAATCCTGGTCGACGGCGACCAGGCGTTCGAAGTACGCCGCCGCGAGACCGTCGCCGAACTGTATGCTGGCGAAAGCCTGCTGCCGGAGt                                                                           <  5:302081/127‑1 (MQ=255)
                                                                                                tGAGCTCCAACTACAACACCCGCGGCCGTTGCGCTGAAATCCTGGTCGACGGCGACCAGGCGTTCGAAGTACGCCGCCGCGAGACCGTCGCCGAACTGTATGCTGGCGAAAGCCTGCTGCCGGAGTAAGCCCATGCTGCTGCGTTTTa                                                     >  5:235510/1‑148 (MQ=255)
                                                                                                      ccAACTACAACACCCGCGGCCGTTGCGCTGAAATCCTGGTCGACGGCGACCAGGCGTTCGAAGTACGCCGCCGCGAGACCGTCGCCGAACTGTATGCTGGCGAAAGCCTGCTGCCGGAGTAAGCCCATGCTGCTGCGTTTTACCaa                                                 >  6:180835/1‑146 (MQ=255)
                                                                                                      ccAACTACAACACCCGCGGCCGTTGCGCTGAAATCCTGGTCGACGGCGACCAGGCGTTCGAAGTACGCCGCCGCGAGACCGTCGCCGAACTGTATGCTGGCGAAAGCCTGCTGCCGGAGTAAGCCCATGCTGCTGCGTTTTACCaa                                                 <  5:180835/146‑1 (MQ=255)
                                                                                                       cAACTACAACACCCGCGGCCGTTGCGCTGAAATCCTGGTCGACGGCGACCAGGCGTTCGAAGTACGCCGCCGCGAGACCGTCGCCGAACTGTATGCTGGCGAAAGCCTGCTGCCGGAGTAAGCCCATGCTGCTGCGTTTTACCAAGa                                               <  8:198270/147‑1 (MQ=255)
                                                                                                        aaCTACAACACCCGCGGCCGTTGCGCTGAAATCCTGGTCGACGGCGACCAGGCGTTCGAAGTACGCCGCCGCGAGACCGTCGCCGAACTGTATGCTGGCGAAAGCCTGCTGCCGGAGTAAGCCCATGCTGCTGCGTTTTACCAAGATg                                             >  4:67502/1‑148 (MQ=255)
                                                                                                           tACAACACCCGCGGCCGTTGCGCTGAAATCCTGGTCGACGGCGACCAGGCGTTCGAAGTACGCCGCCGCGAGACCGTCGCCGAACTGTATGCTGGCGAAAGCCTGCTGCCGGAGTAAGCCCATGCTGCTGCGttt                                                       >  5:313150/1‑135 (MQ=255)
                                                                                                           tACAACACCCGCGGCCGTTGCGCTGAAATCCTGGTCGACGGCGACCAGGCGTTCGAAGTACGCCGCCGCGAGACCGTCGCCGAACTGTATGCTGGCGAAAGCCTGCTGCCGGAGTAAGCCCATGCTGCTGCGttt                                                       <  6:313150/135‑1 (MQ=255)
                                                                                                           tACAACACCCGCGGCCGTTGCGCTGAAATCCTGGTCGACGGCGACCAGGCGTTCGAAGTACGCCGCCGCGAGACCGTCGCCGAACTGTATGCTGGCGAAAGCCTGCTGCCGGAGTAAGCCCATGCTGCTGCGTTTTACCAAGATGCAtg                                         >  5:207431/1‑149 (MQ=255)
                                                                                                           tACAACACCCGCGGCCGTTGCGCTGAAATCCTGGTCGACGGCGACCAGGCGTTCGAAGTACGCCGCCGCGAGACCGTCGCCGAACTGTATGCTGGCGAAAGCCTGCTGCCGGAGTAAGCCCATGCTGCTGCGTTTTACCAAGATGCAtg                                         >  6:257332/1‑149 (MQ=255)
                                                                                                              aacaCCCGCGGCCGTTGCGCTGAAATCCTGGTCGACGGCGACCAGGCGTTCGAAGTACGCCGCCGCGAGACCGTCGCCGAACTGTATGCTGGCGAAAGCCTGCTGCCGGAGTAAGCCCATGCTGCTGCGTTTTACCAAGATGCAtgggc                                      <  7:311403/149‑1 (MQ=255)
                                                                                                              aacaCCCGCGGCCGTTGCGCTGAAATCCTGGTCGACGGCGACCAGGCGTTCGAAGTACGCCGCCGCGAGACCGTCGCCGAACTGTATGCTGGCGAAAGCCTGCTGCCGGAGTAAGCCCATGCTGCTGCGTTTTACCAAGATGCAtgggc                                      <  3:346954/149‑1 (MQ=255)
                                                                                                              aacaCCCGCGGCCGTTGCGCTGAAATCCTGGTCGACGGCGACCAGGCGTTCGAAGTACGCCGCCGCGAGACCGTCGCCGAACTGTATGCTGGCGAAAGCCTGCTGCCGGAGTAAGCCCATGCTGCTGCGTTTTACCAAGATGCAtgggc                                      >  8:297044/1‑149 (MQ=255)
                                                                                                              aacaCCCGCGGCCGTTGCGCTGAAATCCTGGTCGACGGCGACCAGGCGTTCGAAGTACGCCGCCGCGAGACCGTCGCCGAACTGTATGCTGGCGAAAGCCTGCTGCCGGAGTAAGCCCATGCTGCTGCGTTTTACCAAGATGCAtgggc                                      >  5:53407/1‑149 (MQ=255)
                                                                                                               acacCCGCGGCCGTTGCGCTGAAATCCTGGTCGACGGCGACCAGGCGTTCGAAGTACGCCGCCGCGAGACCGTCGCCGAACTGTATGCTGGCGAAAGCCTGCTGCCGGAGTAAGCCCATGCTGCTGCGTTTTACCAAGATGCAtgggct                                     <  2:275975/149‑1 (MQ=255)
                                                                                                                      cgGCCGTTGCGCTGAACTCCTGGTCGACGGCGACCAGGCGTTCGAAGTACGTCGCCGCGAGACCGTCGCCGAACTGTATGCTGGCGAAAGCCTGCTGCCGGAGTAAGCCCATGCTGCTGCGTTTTACCAAGATGCATGGGCTGGGCAAc                              <  3:64749/149‑1 (MQ=255)
                                                                                                                       ggCCGTTGCGCTGAAATCCTGGTCGACGGCGACCAGGCGTTCGAAGTACGCCGCCGCGAGACCGTCGCCGAACTGTATGCTGGCGAAAGCCTGCTGCCGGAGTAAGCCCATGCTGCTGCGTTTTACCAAGATGCATGGGCTGGGCAAc                              >  2:165310/1‑148 (MQ=255)
                                                                                                                          cGTTGCGCTGAAATCCTGGTCGACGGCGACCAGGCGTTCGAAGTACGCCGCCGCGAGACCGTCGCCGAACTGTATGCTGGCGAAAGCCTGCTGCCGGAGTAAGCCCATGCTGCTGCGTTTTACCAAGATGCATGGGCTGGGCAACGACt                          >  6:237668/1‑149 (MQ=255)
                                                                                                                            ttGCGCTGAAATCGTGGTCGACGGCGACCAGGCGTTCGAAGTACGCCGCCGCGAGACCGTCGCCGAACTGTATGCTGGCGAAAGCCTGCTGCCGGAGTAAGCCCATGCTGCTGCGTTTTACCAAGATGCATGGGCTGGGCAACGACTTc                        <  2:357510/149‑1 (MQ=255)
                                                                                                                                    aaaTCCTGGTCGACGGCGACCAGGCGTTCGAAGTACGCCGCAGCGAGACCGTCGCCGAACTGTATGCTGGCGAAAGCCTGCTGCCGGAGTAAGCCCATGCTGCTGCGTTTTACCAAGATGCATGGGCTGGGCAACGACTTCATGGTCCt                <  8:340558/149‑1 (MQ=255)
                                                                                                                                       tCCTGGTCGACGGCGACCAGGCGTTCGAAGTACGCCGCCGCGAGACCGTCGCCGAACTGTATGCTGGCGAAAGCCTGCTGCCGGAGTAAGCCCATGCTGCTGCGTTTTACCAAGATGCATGGGCTGGGCAACGACTTCATGGTCCTGGa             <  5:237668/149‑1 (MQ=255)
                                                                                                                                       gccTGGTCGCCGTCGACCAGGCGTTCGAAGTACGCCGCCGCGAGACCGTCGCCGAACTGTATGCTGGCGAAAGCCTGCTGCCGGAGTAAGCCCATGCTGCTGCGTTTTACCAAGATGCAtgggctgg                                   >  4:25404/2‑127 (MQ=255)
                                                                                                                                       gccTGGTCGCCGTCGACCAGGCGTTCGAAGTACGCCGCCGCGAGACCGTCGCCGAACTGTATGCTGGCGAAAGCCTGCTGCCGGAGTAAGCCCATGCTGCTGCGTTTTACCAAGATGCAtgggctgg                                   <  3:25404/126‑1 (MQ=255)
                                                                                                                                       gccTGGTCGCCGTCGACCAGGCGTTCGAAGTACGCCGCCGCGAGACCGTCGCCGAACTGTATGCTGGCGAAAGCCTGCTGCCGGAGTAAGCCCATGCTGCTGCGTTTTACCAAGATGCATGGGCTGGGCAACGACTTCATGGTCCTGGa             >  3:240819/2‑149 (MQ=255)
                                                                                                                                         cTGGTCGACGGCGACCAGGCGTTCGAAGTACGCCGCCGCGAGACCGTCGCCGAACTGTATGCTGGCGAAAGCCTGCTGCCGGAGTAAGCCCATGCTGCTGCGTTTTACCAAGATGCATGGGCTGGGCAACGACTTCATGGTCCTg               <  6:307492/145‑1 (MQ=255)
                                                                                                                                         cTGGTCGACGGCGACCAGGCGTTCGAAGTACGCCGCCGCGAGACCGTCGCCGAACTGTATGCTGGCGAAAGCCTGCTGCCGGAGTAAGCCCATGCTGCTGCGTTTTACCAAGATGCATGGGCTGGGCAACGACTTCATGGTCCTg               >  5:307492/1‑145 (MQ=255)
                                                                                                                                           ggTCGACGGCGACCAGGCGTTCGAAGTACGCCGCCGCGAGACCGTCGCCGAACTGTATGCTGGCGAAAGCCTGCTGCCGGAGTAAGCCCATGCTGCTGCGTTTTACCAAGATGCATGGGCTGGGCAACGACTTCATGGTCCTGGACCTg         >  4:6198/1‑149 (MQ=255)
                                                                                                                                            gTCGACGGCGACCAGGCGTTCGAAGTACGCCGCCGCGAGACCGTCGCCGAACTGTATGCTGGCGAAAGCCTGCTGCCGGAGTAAGCCCATGCTGCTGCGTTTTACCAAGATGCATGGGCTGGGCAACGACTTCATGGTCCTGGACCt          <  3:67502/147‑1 (MQ=255)
                                                                                                                                            gTCGACGGCGACCAGGCGTTCGAAGTACGCCGCCGCGAGACCGTCGCCGAACTGTATGCTGGCGAAAGCCTGCTGCCGGAGTAAGCCCATGCTGCTGCGTTTTACCAAGATGCATGGGCTGGGCAACGACTTCATGGTCCTGGACCTg         <  1:165310/148‑1 (MQ=255)
                                                                                                                                            gTCGACGGCGACCAGGCGTTCGAAGTACGCCGCCGCGAGACCGTCGCCGAACTGTATGCTGGCGAAAGCCTGCTGCCGGAGTAAGCCCATGCTGCTGCGTTTTACCAAGATGCATGGGCTGGGCAACGACTTCATGGTCCTGGACCTg         <  5:182105/148‑1 (MQ=255)
                                                                                                                                               gACGGCGACCAGGCGTTCGAAGTACGCCGCCGCGAGACCGTCGCCGAACTGTATGCTGGCGAAAGCCTGCTGCCGGAGTAAGCCCATGCTGCTGCGTTTTACCAAGATGCATGGGCTGGGCAACGACTTCATGGTCCTGGACCTGGTca     <  7:297044/149‑1 (MQ=255)
                                                                                                                                               gACGGCGACCAGGCGTTCGAAGTACGCCGCCGCGAGACCGTCGCCGAACTGTATGCTGGCGAAAGCCTGCTGCCGGAGTAAGCCCATGCTGCTGCGTTTTACCAAGATGCATGGGCTGGGCAACGACTTCATGGTCCTGGACCTGGTca     <  3:327839/149‑1 (MQ=255)
                                                                                                                                               gACGGCGACCAGGCGTTCGAAGTACGCCGCCGCGAGACCGTCGCCGAACTGTATGCTGGCGAAAGCCTGCTGCCGGAGTAAGCCCATGCTGCTGCGTTTTACCAAGATGCATGGGCTGGGCAACGACTTCATGGTCCTGGACCTGGTca     <  5:257332/149‑1 (MQ=255)
                                                                                                                                               gACGGCGACCAGGCGTTCGAAGTACGCCGCCGCGAGACCGTCGCCGAACTGTATGCTGGCGAAAGCCTGCTGCCGGAGTAAGCCCATGCTGCTGCGTTTTACCAAGATGCATGGGCTGGGCAACGACTTCATGGTCCTGGACCTGGTc      <  2:77913/148‑1 (MQ=255)
                                                                                                                                                  ggCGACCAGGCGTTCGAAGTACGCCGCCGCGAGACCGTCGCCGAACTGTATGCTGGCGAAAGCCTGCTGCCGGAGTAAGCCCATGCTGCTGCGTTTTACCAAGATGCATGGGCTGGGCAACGACTTCATGGTCCTGGACCTGGTcagcc  <  3:6198/149‑1 (MQ=255)
                                                                                                                                                  ggCGACCAGGCGTTCGAAGTACGCCGCCGCGAGACCGTCGCCGAACTGTATGCTGGCGAAAGCCTGCTGCCGGAGTAAGCCCATGCTGCTGCGTTTTACCAAGATGCATGGGCTGGGCAACGACTTCATGGTCCTGGACCTGGTcagc   >  1:185349/1‑148 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
GCGAGACTGGCGACTTCCTCGGCAAGGACCGCGTCCTCAACCTGGCCGAAGGTGACCTGCTGGCCGTAGAGTCCGCGGGCGCCTATGGTTTTGTCATGAGCTCCAACTACAACACCCGCGGCCGTTGCGCTGAAATCCTGGTCGACGGCGACCAGGCGTTCGAAGTACGCCGCCGCGAGACCGTCGCCGAACTGTATGCTGGCGAAAGCCTGCTGCCGGAGTAAGCCCATGCTGCTGCGTTTTACCAAGATGCATGGGCTGGGCAACGACTTCATGGTCCTGGACCTGGTCAGCC  >  NC_002947/5962129‑5962423

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑