Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,974,855	C→T	7.0%	V25V (GTC→GTT)	PP_5727 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,974,855	0	C	T	7.0%	160.6 / 2.1	58	V25V (GTC→GTT)	PP_5727	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (30/23);  new base T (2/2);  total (32/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.68e-02

GATCCCGCGCGCCTGTTTTTTCGCTAGAACGAACAGAGGGTTATCTTTTGTTTGATAGAAGGCTATTAAAGAATGCGAGACCTATCAGAAAAAGAGCTGTCTCAGATTTCCGGAGGCGACAAGGGCGATACATTTTTTGAAAATGTCGGTGCGACGCTGGGAGGAGCCTTAGGTGCAGCCATCGGATCGAGAACGCCAATTGGTGCAACGGGTGGTGCTGCA‑GTAGGTAGTGGCTTAGGCGCTATAGGAGGTGGAACGGCTTACAATGCTGTCAAGGATGCTGCCAATGCTTG  >  NC_002947/5974709‑5975001                                                                                                                                                   |                                                                                                                                                    gATCCCGCGCGCCTGTTTTTTCGCTAGAACGAACAGAGGGTTATCTTTTGTTTGATAGAAGGCTATTAAAGAATGCGAGACCTATCAGAAAAAGAGCTGTCTCAGATTTCCGGAGGCGACAAGGGCGATACATTTTTTGAAAATGTCg                                                                                                                                                    <  4:290485/148‑1 (MQ=255)    cccGCGCGCCTGTTTTTTAGCTAGAACGAACAGAGGGTTATCTTTTGTTTGATAGAAGGCTATTAAAGAATGCGAGACCTATCAGAAAAAGAGCTGTCTCAGATTTCCGGAGGCGACAAGGTCCATACATTTTGTGAAAATCTCGGTGc                                                                                                                                                >  8:230003/1‑149 (MQ=38)             cTGTTTTTTCGCTAGAACGAACAGAGGGTTATCTTTTGTTTGATAGAAGGCTATTAAAGAATGCGAGACCTATCAGAAAAAGAGCTGTCTCAGATTTCCGGAGGCGACAAGGGCGATACATTTTTTGAAAATGTCGGTGCGACGCTgg                                                                                                                                        >  3:174168/1‑148 (MQ=255)             cTGTTTTTTCGCTAGAACGAACAGAGGGTTATCTTTTGTTTGATAGAAGGCTATTAAAGAATGCGAGACCTATCAGAAAAAGAGCTGTCTCAGATTTCCGGAGGCGACAAGGGCGATACACTTTTTGAAAATGTCGGTGCGACGCTgg                                                                                                                                        >  7:295813/1‑148 (MQ=255)                ttttttCGCTAGAACGAACAGAGGGTTATCTTTTGTTTGATAGAAGGCTATTAAAGAATGCGAGACCTATCAGAAAAAGAGCTGTCTCAGATTTCCGGAGGCGACAAGGGCGATACATTTTTTGAAAATGTCGGTGCGACGCTGggag                                                                                                                                     <  1:17491/148‑1 (MQ=255)                    ttCGCTAGAACGAACAGAGGGTTATCTTTTGTTTGATAGAAGGCTATTAAAGAATGCGAGACCTATCAGAAAGAGAGCTGTCTCAGATTTCCGGAGGCGACAAGGGCGATACATTTTTTGAAAATGTCGGTGCGAGGCTGGGAGGAGcc                                                                                                                                >  3:198886/1‑149 (MQ=255)                     tCGCTAGAACGAACAGAGGGTTATCTTTTGTTTGATAGAAGGCTATTAAAGAATGCGAGACCTATCAGAAAAAGAGCTGTCTCAGATTTCCGGAGGCGACAAGGGCGATACATTTTTTGAAAATGTCGGTGCGACGCTGGGAGGAGcc                                                                                                                                <  1:290934/148‑1 (MQ=255)                         tAGAACGAACAGAGGGTTATCTTTTGTTTGATAGAAGGCTATTAAAGAATGCGAGACCTATCAGAAAAAGAGCTGTCTCAGATTTCCGGAGGCGACAAGGGCGATACATTTTTTGAAAATGTCGGTGAGACTCTGGGAGGAGCATTAgg                                                                                                                           >  8:150288/1‑149 (MQ=255)                             acgaacAGAGGGTTATCTTTTGTTTGATAGAAGGCTATTAAAGAATGCGAGACCTATCAGAAAAAGAGCTGTCTCAGATTTCCGGAGGCGACAAGGGCGATACATTTTTTGAAAATGTCGGTGCGACGCTGGGAGGAGCCTTAGGTGCa                                                                                                                       <  4:118069/149‑1 (MQ=255)                              cgaacAGAGGGTTATCTTTTGTTTGATAGAAGGCTATTAAAGAATGCGAGACCTATCAGAAAAAGAGCTGTCTCAGATTTCCGGAGGCGACAAGGGCGATACATTTTTTGAAAATGTCGGTGCGACGCTGGGAGGAGCCTTAGGTGCAg                                                                                                                      >  8:40485/1‑149 (MQ=255)                                           aTCTTTTGTTTGATAGAAGGCTATTAAAGAATGCGAGACCTATCAGAAAAAGAGCTGTCTCAGATTTCCGGAGGCGACAAGGGCTATACATTTTTTGAAAATGTCGGTGCGACGCTGGGAGGAGCCTTAGGTGCAGCCATCTTATCga                                                                                                          <  7:230003/148‑1 (MQ=255)                                           aTCTTTTGTTTGATAGAAGGCTATTAAAGAATGCGAGACCTATCAGAAAAAGAGCTGTCTCAGATTTCCGCAGGCGACAAGCGCGATACATTTTTTGAAAATGTCGGTGCGACGCTGGGAGGAGCCTTAGGTGCAGCCATCGGATCga                                                                                                          <  6:174407/148‑1 (MQ=255)                                            tCTTTTGTTTGATAGAAGGCTATTAAAGAATGCGAGACCTATCAGAAAAAGAGCTGTCTCAGATTTCCGGAGGCGACAAGGGCGATACATTTTTTGAAAATGTCGGTGCGACGCTGGGAGGAGCCTTAGGTGCAGCCATCGGATCgag                                                                                                         >  1:287479/1‑148 (MQ=255)                                            tCTTTTGTTTGATAGAAGGCTATTAAAGAATGCGAGACCTATCAGAAAAAGAGCTGTCTCAGATTTACGGAGTCGACAAGGGCGATACATTTTTTGAAAATGTCTGTGCGAAGCTGGGAGGAGCCTTAGGTGCAGACATCGGATCgag                                                                                                         >  5:275021/1‑148 (MQ=255)                                                        tAGAAGGCTATTAAAGAATGCGAGACCTATCAGAAAAAGAGCTGTCTCAGATTTCCGGAGGCGACAAGGGCGATACATTTTTTGAAAATGTCGGt                                                                                                                                                  >  8:203076/1‑95 (MQ=255)                                                         aGAAGGCTATTAAAGAATGCGAGACCTATCAGAAAAAGAGCTGTCCCAGATTTCAGGAGTCGACAAGGGCGATACATTTTTTGAAAATGTCGGTGCGACGCTGGGAGTAGCCTTAGTTGCAGCCATCGGATCGAGAAAGCCAATTGGTg                                                                                           >  6:326906/1‑149 (MQ=255)                                                         aGAAGGCTATTAAAGAATGCGAGACCTATCAGAAAAAGAGCAGTCTCAGATTTCCGGAGGCGACAAGGGCGATACATTATTTGAAAATGTCGGTGCGAAGCTCGGAGGAGACTTAGCTGCAGCCATCGTATCCAGAACGCAAATTGGt                                                                                            >  1:81996/1‑148 (MQ=255)                                                          gatgtcTATTAAAGAATGCGAGACCTATCAGAAAAAGAGCTGTCACAGATTTCCGGAGGCGACAAGGGCGATACATTTTTTGAAAATGTCGTTGCGACGCTGGTAGGAGCCTTAGGTGCAGCGATCGGATCGAGAACGGCAATTGGTg                                                                                           <  5:326906/143‑1 (MQ=255)                                                             ggCTATTAAAGAATGCGAGACCTATCAGAAAAAGAGCTGTCTCAGATTTCCGGAGGCGACAAGGGCGATACATTTTTTGAAAATGTCGGTGCGACGCTGGGAGGAGCCTTAGGTGCAGCCATCGGATCGAGAACGCCAATTGGTGCAAc                                                                                       <  4:174168/149‑1 (MQ=255)                                                                tATTAAAGAATGCGAGACCTATCAGAAAAAGAGCTGTCTCAGATTTCCGGAGGCGACAAGGGCGATACATTTTTTGAAAATGTTGGTGCGACGCTGGGAGGAGCCTTAGGTGCAGCCATCGGATc                                                                                                            >  4:139983/1‑125 (MQ=255)                                                                tATTAAAGAATGCGAGACCTATCAGAAAAAGAGCTGTCTCAGATTTCCGGAGGCGACAAGGGCGATACATTTTTTGAAAATGTTGGTGCGACGCTGGGAGGAGCCTTAGGTGCAGCCATCGGATc                                                                                                            <  5:123029/125‑1 (MQ=255)                                                                tATTAAAGAATGCGAGACCTATCAGAAAAAGAGCTGTCTCAGATTTCCGGAGGCGACAAGGGCGATACATTTTTTGAAAATGTTGGTGCGACGCTGGGAGGAGCCTTAGGTGCAGCCATCGGATc                                                                                                            >  6:123029/1‑125 (MQ=255)                                                                tATTAAAGAATGCGAGACCTATCAGAAAAAGAGCTGTCTCAGATTTCCGGAGGCGACAAGGGCGATACATTTTTTGAAAATGTTGGTGCGACGCTGGGAGGAGCCTTAGGTGCAGCCATCGGATc                                                                                                            <  3:139983/125‑1 (MQ=255)                                                                tATTAAAGAATGCGAGACCTATCAGAAAAAGAGCTGTCTCAGATTTCCGGAGGCGACAAGGGCGATACATTTTTTGAAAATGTCGGTGCGACGCTGGGAGGAGCCTTAGGTGCAGCCATCGGATCGAGAACGCCAATTGGTGCAACggg                                                                                    >  3:331094/1‑149 (MQ=255)                                                                tATTAAAGAATGCGAGACCTATCAGAAAAAGAGCTGTCTCAGATTTCCGGAGGCGACAAGGGCGATACATTTTTTGAAAATGTCGGTGCGACGCTGGGAGGAGCCTTAGGTGCAGCCATCGGATCGAGAACGCCAATTGGTGCAACggg                                                                                    >  5:312576/1‑149 (MQ=255)                                                                tATTAAAGAATGCGAGACCTATCAGAAAAAGAGCTGTCTCAGATTTCCGGAGGCGACAAGGGCGATACATTTTTTGAAAATGTCGGTGCGACGCTGGGAGGAGCCTTAGGTGCAGCCATCGGATCGAGAACGCCAATTGGTGCAACggg                                                                                    >  5:123466/1‑149 (MQ=255)                                                                  ttAAAGAATGCGAGACCTATCAGAAAAAGAGCTGTCTCAGATTTCCGGAGGCGACAAGGGCGATACATTTTTTGAAAATGTCGGTGCGACGCTGGGAGGAGCCTTAGGTGCAGCCATCGGATCGAGAACGCCAATTGGTGCAACGggtg                                                                                  <  7:40485/149‑1 (MQ=255)                                                                  ttAAAGAATGCGAGACCTATCAGAAAAAGAGCTGTCTCAGATTTCCGGAGGCGACAAGGGCGATACATTTTTTGAAAATGTCGGTGCGACGCTGGGAGGAGCCTTAGGTGCAGCCATCGGATCGAGAACGCCAATTGGTGCAACGggtg                                                                                  >  5:279486/1‑149 (MQ=255)                                                                    aaaGAATGCGAGACCTATCAGAAAAAGAGCTGTCTCAGATTTCCGGAGGCGACAAGGGCGATACATTTTTTGAAAATGTCGGTGCGACGCTGGGAGGAGCCTTAGGTGCAGCCATCGGATCGAGAACGCCAATTGGTGCAACGggtgg                                                                                 <  2:254640/148‑1 (MQ=255)                                                                        aaTGCGAGACCTATCAGAAAAAGAGCTGTCTCAGATTTCCGGAGGCGACAAGGGCGATACATTTTTTGAAAATGTCGGTGCGACGCTGGGAGGAGCCTTAGGTGCAGCCATCGGATCGAGAACGCCAATTGGTGCAACGGGTGGtgctg                                                                            <  6:279486/149‑1 (MQ=255)                                                                        aaTGCGAGACCTATCACAAAAAGAGCTGTCTCAGATTTCCGGAGGCGACAAGGGCGAAACATTTTTTGAAAATGTCCGTGCGAAGCTGGGAGGAGCATTAGGTGCAGCCATCGGATCGAGAAAGCCAATTGGTGCAACGGGTGGtgatg                                                                            >  4:11951/1‑149 (MQ=255)                                                                             gagaCCTATCAGAAAAAGAGCTGTCTCAGATTTCCGGAGGCGACAAGGGCGATACATTTTTTGAAAATGTCGGTGCGACGCTGGGAGGAGCCTTAGGTGCAGCCATCGGATCGAGAACGCCAATTGGTGCAACGGGTGGTGCTGCA‑g                                                                        >  3:35361/1‑147 (MQ=255)                                                                               gaCCTATCAGAAAAAGAGCTGTCTCAGATTTCCGGAGGCGACAAGGGCGATACACTTTTTGAAAATGTCGGTGCGACGCTGGGAGGAGCCTTAGGTGCAGCCATCGGATCGAGAACGCCAATTGGTGCAACGGGTGGTGCTGCA‑gtagg                                                                    <  8:295813/149‑1 (MQ=255)                                                                                  cTATCAGAAAAAGAGCTGTCTCAGATTTCCGGAGGCGACAAGGGCGATACATTTTTTGAAAATGTCGGTGCGACGCTGGGAGGAGCCTTAGGTGCAGCCATCGGATCGAGAACGCCAATTGGTGCAACGGGTGGTGCTGCA‑gtaggtag                                                                 >  2:28110/1‑149 (MQ=255)                                                                                   tATCAGAAAAAGAGCTGTCTCAGATTTCCGGAGGCGACAAGGGCGATACATTTTTTGAAAATGTCGGTGCGACGCTGGGAGGAGCCTTAGGTGCAGCCATCGGATCg                                                                                                           <  4:346643/107‑1 (MQ=255)                                                                                   tATCAGAAAAAGAGCTGTCTCAGATTTCCGGAGGCGACAAGGGCGATACATTTTTTGAAAATGTCGGTGCGACGCTGGGAGGAGCCTTAGGTGCAGCCATCGGATCg                                                                                                           >  3:346643/1‑107 (MQ=255)                                                                                    aTCAGAAAAAGAGCTGTCTCAGATTTCCGGAGGCGACAAGGGCGATACATTTTTTGAAAATGTCGGTGCGACGCTGGGAGGAGCCTTAGGTGCAGCCATCTGATCGAGAACGCCAATTGGTGCAACGGGTGGTGCTGCA‑GTAGGTAGTg                                                               >  5:353643/1‑149 (MQ=255)                                                                                        gAAAAAGAGCTGTCTCAGATTTCCGGAGGCGACAAGGGCGATACATTTTTTGAAAATGTCGGTGCGACGCTGGGAGGAGCCTTAGGTGCAGCCATCGGATCGAGAACGCCAATTGGTGCAACGGGTGGTGCTGCA‑GTAGGTAGTGGCt                                                            >  5:107392/1‑148 (MQ=255)                                                                                        gAAAAAGAGCTGTCTCAGATTTCCGGAGGCGACAAGGGCGATACATTTTTTGAAAATGTCGGTGCGACGCTGGGAGGAGCCTTAGGTGCAGCCATCGGATCGAGAACGCCAATTGGTGCAACGGGTGGTGCTGCA‑GTAGGTAGTGGCt                                                            >  2:197251/1‑148 (MQ=255)                                                                                          aaaaGAGCTGTCTCAGATTTCCGGAGGCGACAAGGGCTATACATTTTTTGAAAATGTCGGTGCGACGCTGGGAGGAGCCTTAGGTGCAGCCATCGGATCGAGAACGCCAATTGGTGCAACGGGTGGTGCTGCA‑GTAGGTAGTGGCTTa                                                          <  4:331094/148‑1 (MQ=255)                                                                                             agagCTCTCTCAGATTTCCGGAGGCGACAAGGGCGATACATTTTTTGAAAATGTCGGAGCGACGCTGGGAGGAGCCTTAGGTGAAGCCATCGGATCGAGAACGCCAATAGGTGCAACGGGTGGTGCTCAA‑GTAGGTACTGGCTAAGgcg                                                      >  2:74179/1‑149 (MQ=255)                                                                                                gCTGTCTCAGATTTCCGGAGGCGACAAGGGCGATACATTTTTTGAAAATGTCGGTGCGACGCTGGGAGGAGCCTTAGGTGCAGCCATCGGATCGAGAACGCCAATTGGTGCAACGGGTGGTGCTGCA‑GTAGGTAGTGGCTTAGGCGCta                                                   <  1:303277/149‑1 (MQ=255)                                                                                                 cTGTCTCAGATTTCCGGAGGCGACAAGGGCGATACATTTTTTGAAAATGTCGGTGCGACGCTGGGAGGAGCCTTAGGTGCAGCCATCGGATCGAGAACGCCAATTGGTGCAACGGGTGGTGCTGCA‑GTAGGTAGTGGCTTAGGCGCtat                                                  <  6:107392/149‑1 (MQ=255)                                                                                                  tGTCTCAGATTTCCGGAGGCGACAAGGGCGATACATTTTTTGAAAATGTCGGTGCGACGCTGGGAGGAGCCTTAGGTGCAGCCATCGGATCGAGaa                                                                                                       <  6:140827/96‑1 (MQ=255)                                                                                                  tGTCTCAGATTTCCGGAGGCGACAAGGGCGATACATTTTTTGAAAATGTCGGTGCGACGCTGGGAGGAGCCTTAGGTGCAGCCATCGGATCGAGaa                                                                                                       >  5:140827/1‑96 (MQ=255)                                                                                                    tctcAGATTTCCGGAGGCGACAAGGGCGATACATTTTTTGAAAATGTCGGTGCGACGCTGGGAGGAGCCTTAGGTGCAGCCATCGGATCGAGAACGCCAATTGGTGCAACGGGTGGTGCTGCA‑GTAGGTAGTGGCTTAGGCGCTATag                                                >  8:8891/1‑148 (MQ=255)                                                                                                                ggAGGCGACAAGGGCGATACATTTTTTGAAAATGTCGGTGCGACGCTGGGAGGAGCCTTAGGTGCAGCCATCGGATGGAGAACGCCATTTGGTGCAACGGGTGGTGCTGGA‑GTAGGTAGTGGCTTAGGCGCTATAGGAGGTGGAACGGc                                   >  2:315517/1‑149 (MQ=255)                                                                                                                 gAGGCGACAAGGGCGATACATTTTTTGAAAATGTCGGTGCGACGCTGGGAGGAGCCTTAGGTGCAGCCATCGGATCGAGAACGCCAATTGGTGCAACGGGTGGTGCTGCA‑GTAGGTAGTGGCTTAGGCGCTATAGGAGGTGGAACGGCt                                  >  8:340209/1‑149 (MQ=255)                                                                                                                    gCGACAAGGGCGATACATTTTTTGAAAATGTCGGTGCGACGCTGGGAGGAGCCTTAGGTGCAGCCATCGGATCGAGAACGCCAATTGGTGCAACGGGTGGTGCTGCA‑GTAGGTAGTGGCTTAGGCGCTATAGGAGGTGGAACGGCTTAc                               <  6:123466/149‑1 (MQ=255)                                                                                                                        cAAGGGCGATACATTTTTTGAAAATGTGGGTGCGACGCTGGGAGGAGCCTTAGGTGCAGCCATCGGATCGAGAACGCCAATTGGTGCAACGGGTGGTGCTGCACGTAGGTAGTGGCTTATGCGCTATAGGAGGTGGAACGGCTTACAAt                            <  1:74179/149‑1 (MQ=255)                                                                                                                        cAAGGGCGATACATTTTTTGAAAATGTCGGTGCGACGCTGGGAGGAGCCTTAGGTGCAGCCATCGGATCGAGAACGCCAATTGGTGCAACGGGTGGTGCTGCA‑GTAGGTAGTGGCTTAGGCGCTATAGGAGGTGGAACGGCTTACAATg                           <  7:8891/149‑1 (MQ=255)                                                                                                                        cAAGGGCGATACATTTTTTGAAAATGTCGGTGCGACGCTGGGAGGAGCCTTAGGTGCAGCCATCGGATCGAGAACGCCAATTGGTGCAACGGGTGGTGCTGCA‑GTAGGTAGTGGCTTAGGCGCTATAGGAGGTGGAACGGCTTACAATg                           <  1:28110/149‑1 (MQ=255)                                                                                                                          aGGGCGATACATTTTTTGAAAATGTCGGTGCGACGCTGGGAGGAGCCTTAGGTGCAGCCATCGGATCGAGAACGCCAATTGGTGCAACGGGTGGTGCTGCA‑GTAGGTAGTGGCTTAGGCGCTATAGGAGGTGGAACGGCTTACAATGCt                         <  4:35361/149‑1 (MQ=255)                                                                                                                               gATACATTTTTTGAAAATGTCGGTGCGACGCTGGGAGGAGCCTTAGGTGCAGCCATCGGGTCGAGAACGCCAATTGGTGCAACGGGTGGTGCTGCA‑GTAGGTAGTGGCTTAGGCGCTATAGGAGGTGGAACGGCTTACAATGCTGTc                      >  1:45870/1‑147 (MQ=255)                                                                                                                                   cATTTTTTGAAAATGTCGGTGCGACGCTGGGAGGAGCCTTAGGTACAGCCATCGGATCGAGAACGCCAATTGGTGCAACGGGTGGTGCTGCA‑GTAGGTAGTGGCTTAGGCGCTATAGGAGGTGGAACGGCTTACAATGCTGTCAAGGAt                >  7:271193/1‑149 (MQ=255)                                                                                                                                    aTTTTTTGAAAATGTCGGTGCGACGCTGGGAGGAGCCTTAGGTGCAGCCATCGGATCGAGAACGCCAATTGGTGCAACGGGTGGTGCTGCA‑GTAGGTAGTGGCTTAGGCGCta                                                   <  8:333634/113‑1 (MQ=255)                                                                                                                                           gAAAATGTCGGTGCGACGCTGGGAGGAGCCTTAGGTGCAGCCATCGGATCGAGAACGCCAATTGGTGCAACGGGTGGTGCTGCA‑GTAGGTAGTGGCTTAGGCGCTATAGGAGGTGGAACGGCTTACAATGCTGTCAAGGATGCAGCCaa        >  3:43378/1‑149 (MQ=255)                                                                                                                                                  tCGGTGCGAAGCTGGGAGGAGCCTTAGGTGCAGCCATCGGATCGAGAACTCCAATTGGTGCAACGGGTGGTGCTGCA‑GTAGGTAGTGGCTTAGGCGCTATAGGAGGTGGAACGGCTTACAATGCTGTCAAGGATGCTGCCAATGCTTg  <  6:353643/148‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                    GATCCCGCGCGCCTGTTTTTTCGCTAGAACGAACAGAGGGTTATCTTTTGTTTGATAGAAGGCTATTAAAGAATGCGAGACCTATCAGAAAAAGAGCTGTCTCAGATTTCCGGAGGCGACAAGGGCGATACATTTTTTGAAAATGTCGGTGCGACGCTGGGAGGAGCCTTAGGTGCAGCCATCGGATCGAGAACGCCAATTGGTGCAACGGGTGGTGCTGCA‑GTAGGTAGTGGCTTAGGCGCTATAGGAGGTGGAACGGCTTACAATGCTGTCAAGGATGCTGCCAATGCTTG  >  NC_002947/5974709‑5975001

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑