| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,986,861 | C→T | 100% | intergenic (+141/‑58) | PP_5245 → / → kefB‑III | AraC family transcriptional regulator/glutathione‑regulated potassium/H+ antiporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,986,861 | 0 | C | T | 90.2% | 133.5 / 5.8 | 52 | intergenic (+141/‑58) | PP_5245/kefB‑III | AraC family transcriptional regulator/glutathione‑regulated potassium/H+ antiporter |
| Reads supporting (aligned to +/- strand): ref base C (4/1); new base T (25/21); total (29/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.75e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.34e-01 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
TTAGCGCTGCCTTCTTCGCGGGCGCGCCCGCTCCCACAGAGATAGCACAGGCCTTGAGCCCAGGTAAGC‑GCCGGCACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGTCTGACGCCACACTACCCCTCAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGCCACACGAAGGCAGCCTTCTGCAAACCGCGGTGATCTTCCTGCTTGCCGCCGTCCTCGCCGTCCCCCTGGCCAAGCGCCTG > NC_002947/5986717‑5987002 | ttAGCGCTGCCTTCTTCGCGGGCGCGCCCGCTCCCACAGAGATAGCACAGGCCTTGAGCCCAGGTAAGC‑GCCGGCACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGTCTGACGCCACACTACCCCTTAGaa < 2:190991/149‑1 (MQ=255) ccTTCTTCGCGGGCGCGCCCGCTCCCACAGAGATAGCACAGGCCTTGAGCCCAGGTAAGC‑GCCGGCACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGTCTGACGCCACACTACCCCTTAGAATTGTCCGTc < 3:3762/149‑1 (MQ=255) ttcttcGCGGGCGCGCCCGCTCCCACAGAGATAGCACAGGCCTTGAGCCCAGGTAAGC‑GCCGGCACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGTCTGACGCCACACTACCCCTTAGAATTGTCCGTCa > 7:64211/1‑148 (MQ=255) tcttcGCGGGCGCGCCCGCTCCCACAGAGATAGCACAGGCCTTGAGCCCAGGTAAGCGGCCGGCACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGTCTGACGCCACACTACCCCTTAGAATTGTCCGTCa > 1:281264/1‑148 (MQ=255) gcgGGCGCGCCCGCTCCCACAGAGATAGCACAGGCCTTGAGCCCAGGTAAGC‑GCCGGCACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGTCTGACGCCACACTACCCCTTAGAATTGTCCGTCAAACTGtt < 6:115686/149‑1 (MQ=255) gcgGGCGCGCCCGCTCCCACAGAGATAGCACAGGCCTTGAGCCCAGGTAAGC‑GCCGGAACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGTCTGACGCCACACTACCCCTTAGAATTGTCCGTCAAACTGtt < 7:193303/149‑1 (MQ=255) ccGCTCCCACAGAGATAGCACAGGCCTTGAGCCCAGGTAAGC‑GCCGGCACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGTCTGACGCCACACTACCCCTTAGAATTGTc > 1:237828/1‑127 (MQ=255) ccGCTCCCACAGAGATAGCACAGGCCTTGAGCCCAGGTAAGC‑GCCGGCACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGTCTGACGCCACACTACCCCTTAGAATTGTc < 2:237828/127‑1 (MQ=255) tCCCACAGAGATAGCACAGGCCTTGAGCCCAGGTAAGC‑GCCGGCACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGTCTGACGCCACACTACCCCTTAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCg > 8:52090/1‑149 (MQ=255) tCCCACAGAGATAGCACAGGCCTTGAGCCCAGGTAAGC‑GCCGGCACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGACTGACGCCACACTACCCCTCAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCg > 3:124148/1‑149 (MQ=255) ccACAGAGATAGCACATGCCTTGAGCCCAGGTAAGC‑GCCGTCACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGTCTGACGCCACACTACCCCTTAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCg < 3:262419/147‑1 (MQ=255) ccACAGAGATAGCACAGGCCTTGAGCCCAGGTAAGC‑GCCGGCACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGTCTGACGCCACACTACCCCTTAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCgg < 4:166151/148‑1 (MQ=255) ccACAGAGATAGCACAGGCCTTGAGCCCAGGTAAGC‑GCCGGCACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGTCTGACGCCACACTACCCCTTAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGc > 2:112171/1‑149 (MQ=255) acaGAGATAGCACAGGCCTTGAGCCCAGGTAAGC‑GCCGGCACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGTCTGACGCCACACTACCCCTTAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAg > 3:347603/1‑149 (MQ=255) tAGCACAGGCCTTGAGCCCAGGTAAGC‑GCCGGCACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGTCTGACGCCACACTACCCCTTAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATgg < 4:347603/149‑1 (MQ=255) gCACAGGCCTTGAGCCCAGGTAAGC‑GCCGGCACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGTCTGACGCCACACTACCCCTTAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGa < 8:64211/148‑1 (MQ=255) gCACAGGCCTTGACCCCAGGTAAGC‑GCCGGGACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGTCTGACGCCACACTACCCCTTAGAATTGTCCGTCAAACTGTTCGAACGCTCCCCCGGCAGCTCATGGa < 3:277962/148‑1 (MQ=255) acaGGCCTTGAGCCCAGGTAAGC‑GCCGGCACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGTCTGACGCCACACTACCCCTTAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTa > 6:244363/1‑149 (MQ=255) acaGGCCTTGAGCCCAGGTAAGC‑GCCGGCACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGTCTGACGCCACACTACCCCTTAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTa < 2:217976/149‑1 (MQ=255) gCCTTGAGCCCAGGTAAGC‑GCCGGCACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGTCTGACGCCACACTACCCCTTAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAg > 7:279848/1‑149 (MQ=255) ttGAGCCCAGGTAAGC‑GCCGGCACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGTCTGACGCCACACTACCACTTAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGcc > 6:201054/1‑148 (MQ=255) aGGTAAGC‑GCCGGCACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGTCTGACGCCACACTACCCCTTAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGCcaca > 3:69927/1‑149 (MQ=255) aCCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGACTGACGCCACACTACCCCTCAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGCCACACGAAGGCAGCCTTc < 6:99863/149‑1 (MQ=255) gATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGTCTGACGCCACACTACCCCTTAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGCCACACGAAGGCAGCCTTCTg > 2:89679/1‑148 (MQ=255) aTTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGCCTGACGCCACACTACCCCTTAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGCCACACGAAGGCAGCCTTCTGc > 2:292793/1‑148 (MQ=255) ttttaaCTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGTCTGACGCCACACTACCCCTTAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGCCACACGAAGGc < 7:340674/132‑1 (MQ=255) ttGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGTCTGACGCCACACTACCCCTTAGAATTGTCCGTCAAACTGTTCGAACGCTGCCACGGCAGATCATGGAGTACGAGCCAATGCAACACGAAGGc > 8:340674/1‑137 (MQ=255) gTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGTCTGACGCCACACTACCCCTTAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGCCACACGAAGGCAGCCTTCTGCaaa < 5:244363/148‑1 (MQ=255) ctctGGACAGGACAATTTTTCTTCCCGTCAGATGATTGTCTGACGCCACACTACCCCTTAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGCCACACGAAGGCAGCCTTCTGCAAACcgcg > 8:128448/1‑149 (MQ=255) ctctGGACAGGACAATTTTTCTTCCCGTCAGATGATTGTCTGACGCCACACTACCCCTTAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGCCACACGAAGGCAGCCTTCTGCAAACcgcg > 1:309877/1‑149 (MQ=255) gacaATTTTTCTTCCCGTCAGATGATTGTCTGACGCCACACTACCCCTTAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGCCACACGAAGGCAGCCTTCTGCAAACCGCGGTGATCTTcc > 5:164483/1‑149 (MQ=255) gacaATTTTTCTTCCCGTCAGATGATTGTCTGACGCCACACTACCCCTTAGAATTGTCCATCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAAt > 8:236933/1‑108 (MQ=255) gacaATTTTTCTTCCCGTCAGATGATTGTCTGACGCCACACTACCCCTTAGAATTGTCCATCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAAt < 7:236933/108‑1 (MQ=255) aaTTTTTCTTCCCGTCAGATGATTGTCTGACGCCACACTACCCCTTGGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGCCACACGAAGGCAGCCTTCTGCAAACCGCGGTGATCTTc > 6:265391/1‑145 (MQ=255) aaTTTTTCTTCCCGTCAGATGATTGTCTGACGCCACACTACCCCTTGGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGCCACACGAAGGCAGCCTTCTGCAAACCGCGGTGATCTTc < 5:265391/145‑1 (MQ=255) aaTTTTTCTTCCCGTCAGATGATTGTCTGACGCCACACTACCCCATAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGCCACACGAAGGCAGCCTTCTGCAAACCGCGGTGATCTTCCTGc > 5:168634/1‑149 (MQ=255) tttttCTTCCGGTCTGATGATTGTCTGACGCCACTCTACCCCTTAGAATTGTCCGTCATACTGTTCGAAGGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGCCACACGAAGGCAGCCTTCTGCAAACCGCGGTGATCTTCCTGCtt < 6:168634/149‑1 (MQ=255) ttcCCGTCAGATGATTGTCTGACGCCACACTACCCCTTAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGCCACACGAAGGCAGCCTTCTGCAAACAGCGGTGATCTTCCTGCTTgccgcc > 6:325248/1‑149 (MQ=255) ttcCCGTCAGATGATTGACTGACGCCACACTACCCCTCAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGCCACACGAAGGCAGCCTTCTGCAAACCGCGGTGATCTTCCTGCTTgccgcc > 3:321719/1‑149 (MQ=255) tCAGATGATTGTCTGACGCCACACTACCCCTTAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGCCACACGAAGGCAGCCTTCTGCAAACCGCGGTGATCTTCCTGCTTGCCGCCGTCCTc < 2:309877/149‑1 (MQ=255) aGATGATTGTCTGACGCCACACTACCCCTTAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGCCACACGAAGGCAGCCTTCTGCAAACCGCGGTGATCTTCCTGCTTGCCGCCGTCCTCGc < 8:279848/149‑1 (MQ=255) aGATGATTGCCTGACGCCACACTACCCCTTAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGCCACACGAAGGCAGCCTTCTGCAAACCGCGGTGATCTTCCTGCTTGCCGCCGTCCTCGc < 1:292793/149‑1 (MQ=255) gatgatTGACTGACGCCACACTACCCCTCAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGCCACACGAAGGCAGCCTTCTGCAAACCGCGGTGATCTTCCTGCTTGCCGCCGTCCTCGcc > 4:319994/1‑149 (MQ=255) atgatTGTCTGACGCCACACTACCCCTTAGAATTGTACGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGCCACACGAAGGCAGCCTTATGCAAACCGCGGTGATCTTCCTGCTTGCCGCCGTCCTCGCCg > 7:228344/1‑149 (MQ=255) ttGACTGACGCCACACTACCCCTCAGAATTGTCCGTCAAACTGTTCGAACGCTGCCACTGCAGCTCATGGAGTACGACCCAATGCCACACGAAGGCAGCCTTCTGCAAACCGCGGTGATCTTACTCCTTGACGCCGTCCTCGCAGTccc > 6:217862/1‑149 (MQ=255) tCTGACGCCACACTACCCCTTAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGCCACACGAAGGCAGCCTTCTGCAAACCGCGGTGATCTTCCTGCTTGCCGCCGTCCTCGCCGTCCCCCt > 8:285333/1‑149 (MQ=255) gACGCCACACTACCCCTTAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGCCACACGAAGGCAGCCTTCTGCAAACCGCGGTGATCTTCCTGCTTGCCGCCGTCCTCGCCGTCCCCCTGGc > 2:56024/1‑149 (MQ=255) aCGCCACACTACCCCTGAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGCCACACGAAGGCAGCCTTCTGCAAACCGCGGTGATCTTCCTGCTTGCCGCCGTCCTCGCCGTCCCCCTGGcc < 5:217862/149‑1 (MQ=255) acTACCCCTTAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGc < 6:261194/65‑1 (MQ=255) acTACCCCTTAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGc > 5:261194/1‑65 (MQ=255) cTACCCCTTAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGCCACACGAAGGCAGCCTTCTGCAAACCGCGGTGATCTTCCTGCTTGCCGCCGTCCTCGCCGTCCCCCTGGCCAAGCGCCt < 6:164483/149‑1 (MQ=255) aCCCCTTAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGCCACACGAAGGCAGCCTTCTGCAAACCGCGGTGATCTTCCTGCTTGCCGCCGTCCTCGCCGTCCCCCTGGCCAAGCGCCTg > 5:160986/1‑148 (MQ=255) | TTAGCGCTGCCTTCTTCGCGGGCGCGCCCGCTCCCACAGAGATAGCACAGGCCTTGAGCCCAGGTAAGC‑GCCGGCACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGTCTGACGCCACACTACCCCTCAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGCCACACGAAGGCAGCCTTCTGCAAACCGCGGTGATCTTCCTGCTTGCCGCCGTCCTCGCCGTCCCCCTGGCCAAGCGCCTG > NC_002947/5986717‑5987002 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |