Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 6,003,474 C→T 5.6% V155M (GTG→ATG)  amaB ← L‑piperidine‑6‑carboxylate dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029476,003,4740CT 5.6% 215.2 / 2.3 71V155M (GTG→ATG) amaBL‑piperidine‑6‑carboxylate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base C (30/37);  new base T (2/2);  total (32/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.59e-01

GCCAAGCCAGCCGGAGCATCGCCGAAGGCTTTCAGGGCTTTTTCGAACAACGCCTGGCAGGCCAGGGCGGTCAGCGGAGTCTTCTCGGACGGTTTCCATACCACCGAGTTGCCGGCCACCAGGGCCAGGGCGGTGTTCCAGGCCCACACGGCAACCGGGAAGTTGAAGGCGCTGATCACGCCGACCACACCCAGCGGGTGCCAGGTTTCACGCATGTGGTGGCCCGGGCGCTCGGAAGCGATGGTCAGGCCGTACAGCTGGCGCGACAGGCCAACGGCGAAGTCGCAGATGTCGATC  >  NC_002947/6003326‑6003622
                                                                                                                                                    |                                                                                                                                                    
gCCAAGCCAGCCGGAGCATCGCCGAAGGCTTTCAGGGCTTTTTCGAACAACGCCTGGCAGGCCAGGGCGGTCAGCGGAGTCTTCTCGGACGGTTTCCATACCACCGAGTTGCCGGCCACCAGGGCCAGGGCGGTGTTCCAGGCCCacac                                                                                                                                                      >  3:184761/1‑149 (MQ=255)
 ccAAGCCAGCCGGAGCATCGCCGAAGGCTTTCAGGGCTTTTTCGAACACCGCCTGGCAGGCCAGGGCGGTCAGCGGAGTCTTCTCGGACGGTTTCCATACCACCGAGTTGCCGGCCACCAGGGCCAGGGCGGTGTTCCAGGCCCACACg                                                                                                                                                     <  6:118182/149‑1 (MQ=255)
  cAAGCCAGCCGGAGCATCGCCGAAGGCTTTCAGGGCTTTTTCGAACAACGCCTGGCAGGCCAGGGCGGTCAGCGGAGTCTTCTCGGACGGTTTCCATACCACCGAGTTGCCGGCCACCAGGGCCAGGGCGGTGTTCCAGGCCCACACgg                                                                                                                                                    >  1:233374/1‑149 (MQ=255)
  cAAGCCAGCCGGAGCATCGCCGAAGGCTTTCAGGGCTTTTTCGAACAACGCCTGGCAGGCCAGGGCGGTCAGCGGAGTCTTCTCGGACGGTTTCCATACCACCGAGTTGCCGGCCACCAGGGCCAGGGCGGTGTTCCAGGCCCACACgg                                                                                                                                                    >  7:22078/1‑149 (MQ=255)
  cAAGCCAGCCGGAGCATCGCCGAAGGCTTTCAGGGCTTTTTCGAACAAAGCCTGGCAGGCCAGGGCGGTCAGCGGAGTCTTCTCGGACGGTTTCCATACCACCGAGTTGCCGGCCACCAGGTCCAGGGCGGTGTTCCAGGCCCACACgg                                                                                                                                                    >  1:358480/1‑149 (MQ=255)
    agccagccGGAGCATCGCCGAAGGCTTTCAGGGCTTTTTCGAACAACGCCTGGCAGGCCAGGGCGGTCAGCGGAGTCTTCTCGGACGGTTTCCATACCACCGAGTTGCCGGCCACCAGGGCCAGGGCGGTGTTCCAGGACCACACGGc                                                                                                                                                   >  7:203904/1‑148 (MQ=255)
           cGGAGCATCGCCGAAGGCTTTCAGGGCTTTTTCGAACAACGCCTGGCAGGCCAGGGCGGTCAGCGGAGTCTTCTCGGACGGTTTCCATACCACCGAGTTGCCGGCCACCAGGGCCAGGGCGGTGTTCCAGGCCCACACGGCAACCggg                                                                                                                                            >  6:330788/1‑148 (MQ=255)
            ggAGCATCGCCGAAGGCTTTCAGGGCTTTTTCGAACAACGCCTGGCAGGCCAGGGCGGTCAGCGGAGTCTTCTCGGACGGTTTCCATACCACCGAGTTGCCGGCCACCAGGGCCAGGGCGGTGTTCCAGGCCCACACGGCAACCGGGaa                                                                                                                                          <  8:203904/149‑1 (MQ=255)
            ggAGCATCGCCGAAGGCTTTCAGGGCTTTTTCGAACAACGCCTGGCAGGCCAGGGCGGTCAGCGGAGTCTTCTCGGACGGTTTCCATACCACCGAGTTGCCGGCCACCAGGGCCAGGGCGGTGTTCCAGGCCCACACGGCAACCGGGaa                                                                                                                                          <  2:206464/149‑1 (MQ=255)
                 aTCGCCGAAGGCTTTCAGGGCTTTTTCGAACAACGCCTGGCAGGCCAGGGCGGTCAGCGGAGTCTTCTCGGACGGTTTCCATACCACCGAGTTGCCGGCCACCAGGGCCAGGGCGGTGTTCCAGGCCCACACGGCAACCGGGAAGTTg                                                                                                                                      >  7:299903/1‑148 (MQ=255)
                 aTCGCCGAAGGCTTTCAGGGCTTTTTCGAACAACGCCTGGCAGGCCAGGGCGGTCAGCGGAGTCTTCTCGGACGGTTTCCATACCACCGAGTTGCCGGCCACCAGGGCCAGGGCGGTGTTCCAGGCCCACACGGCAACCGGGAAGTTg                                                                                                                                      >  5:133455/1‑148 (MQ=255)
                     ccGAAGGCTTTCAGGGCTTTTTCGAACAACGCCTGGCAGGCCAGGGCGGTCAGCGGAGTCTTCTCGGACGGTTTCCATACCACCGAGTTGCCGGCCACCAGGGCCAGGGCGGTGTTCCAGGCCCACACGGCAACCGGGAAGTTGAAGgc                                                                                                                                 <  8:299903/149‑1 (MQ=255)
                        aaGGCTTTCAGGGCTTTTTCGAACAACGCCTGGCAGGCCAGGGCGGTCAGCGGAGTCTTCTCGGACGGTTTCCATACCACCGAGTTGCCGGCCACCAGGGCCAGGGCGGTGTTCCAGGCCCACACGGCAACCGGGAAGTTGAAGGCGCt                                                                                                                              >  7:152773/1‑149 (MQ=255)
                         aGGCTTTCAGGGCTTTTTCGAACAACGCCTGGCAGGCCAGGGCGGTCAGCGGAGTCTTCTCGGACGGTTTCCATACCACCGAGTTGCCGGCCACCAGGGCCAGGGCGGTGTTCCAGGCCCACACGGCAACCGGGAAGTTGAAGGCGCTg                                                                                                                             <  8:85772/149‑1 (MQ=255)
                           gCTTTCAGGGCTTTTTCGAACAACGCCTGGCAGGCCAGGGCGGTCAGCGGAGTCTTCTCGGACGGTTTCCATACCACCGAGTTGCCGGCCACCAGGGCCAGGGCGGTGTTCCAGGCCCACACGGCAACCGGGAAGTTGAAGGCGCTGAt                                                                                                                           <  3:7551/149‑1 (MQ=255)
                               tCAGGGCTTTTTCGAACAACGCCTGGCAGGCCAGGGCGGTCAGCGGAGTCTTCTCGGACGGTTTCCATACCACCGAGTTGCCGGCCACCAGGGCCAGGGCGGTGTTCCAGGCCCACATGGCAACCGGGAAGTTGAAGGCGCTGATCACg                                                                                                                       >  5:125526/1‑149 (MQ=255)
                               tCAGGGCTTTTTCGAACAACGCCTGGCAGGCCAGGGCGGTCAGCGGAGTCTTCTCGGACGGTTTCCATACCACCGAGTTGCCGGCCACCAGGGCCAGGGCGGTGTTCCAGGCCCACACGGCAACCGGGAAGTTGAAGGCGCTGATCACg                                                                                                                       >  3:199433/1‑149 (MQ=255)
                                  gggCTTTTTCGAACAACGCCTGGCAGGCCAGGGCGGTCAGCGGAGTCTTCTCGGACGGTTTCCATACCACCGAGTTGCCGGCCACCAGGGCCAGGGCGGTGTTCCAGGCCCACACGGCAACCGGGAAGTTGAAGgcgc                                                                                                                               >  1:318056/1‑138 (MQ=255)
                                  gggCTTTTTCGAACAACGCCTGGCAGGCCAGGGCGGTCAGCGGAGTCTTCTCGGACGGTTTCCATACCACCGAGTTGCCGGCCACCAGGGCCAGGGCGGTGTTCCAGGCCCACACGGCAACCGGGAAGTTGAAGgcgc                                                                                                                               <  2:318056/138‑1 (MQ=255)
                                    gCTTTTTCGAACAACGCCTGGCAGGCCAGGGCGGTCAGCGGAGTCTTCTCGGACGGTTTCCATACCACCGAGTTGCCGGCCACCAGGGCCAGGGCGGTGTTCCAGGCCCACACGGCAACCGGGAAGTTGAAGGCGCTGATCACGCCGAc                                                                                                                  <  5:157878/149‑1 (MQ=255)
                                    gCTTTTTCGAACAACGCCTGGCAGGCCAGGGCGGTCAGCGGAGTCTTCTCGGACGGTTTCCATACCACCGAGTTGCCGGCCACCAGGGCCAGGGCGGTGTTCCAGGCCCACACGGCAACCGGGAAGTTGAAGGCGCTGATCACGCCGAc                                                                                                                  <  3:138661/149‑1 (MQ=255)
                                         ttCGAACAACGCCTGGCAGGCCAGGGCGGTCAGCGGAGTCTTCTCGGACGGTTTCCATACCACCGAGTTGCCGGCCACCAGGGCCAGGGCGGTGTTCCAGGCCCACATGGCAACCGGGAAGTTGAAGGCGCTGATCACGCCGACCacac                                                                                                             >  7:172906/1‑149 (MQ=255)
                                             aacaacGCCTGGCAGGCCAGGGCGGTAAGCGGAGTCTTCTCGGACGGTCTCCATACCACCGAGTTCCCGGCCACCAGGGCCAGGGCGGTGTTCCAGGCCCACACGGCAACCGGGAAGTTGAAGGCGCTGATCACGCCGACCACACCCAg                                                                                                         >  4:212177/1‑149 (MQ=255)
                                                aacGCCTGGCAGGCCAGGGCGGTCAGCGGAGTCTTCTCGGACGGTTTCCATACCACCGAGTTGCCGGCCACCAGGGCCAGGGCGGTGTTCCAGGCCCACACGGCAACCGGGAAGTTGAAGGCGCTGATCACGCCGACCACACCCAGCgg                                                                                                      >  6:13027/1‑149 (MQ=255)
                                                  cGCCTGGCAGGCCAGGGCGGTCAGCGGAGTCTTCTCGGACGGTTTCCATACCACCGAGTTGCCGGCCACCAGGGCCAGGGCGGTGTTCCAGGCCCACACGGCAACCGGGAAGTTGAAGGCGCTGATCACGCCGACCACACCCAGCGGGt                                                                                                    <  7:188769/149‑1 (MQ=255)
                                                     cTGGCAGGCCAGGGCGGTCAGCGGAGTCTTCTCGGACGGTTTCCATACCACCGAGTTGCCGGCCACCAGGGCCAGGGCGGTGTTCCAGGCCCACACGGCAACCGGGAAGTTGAAGGCGCTg                                                                                                                             >  3:164686/1‑121 (MQ=255)
                                                     cTGGCAGGCCAGGGCGGTCAGCGGAGTCTTCTCGGACGGTTTCCATACCACCGAGTTGCCGGCCACCAGGGCCAGGGCGGTGTTCCAGGCCCACACGGCAACCGGGAAGTTGAAGGCGCTg                                                                                                                             <  4:164686/121‑1 (MQ=255)
                                                        gCAGGCCAGGGCGGTCAGCGGAGTCTTCTCGGACGGTTTCCATACCACCGAGTTGCCGGCCACCAGGGCCAGGGCGGTGTTCCAGGCCCACACGGCAACCGGGAAGTTGAAGGCGCTGATCACGCCGACCACACCCAGc                                                                                                        >  1:108966/1‑139 (MQ=255)
                                                        gCAGGCCAGGGCGGTCAGCGGAGTCTTCTCGGACGGTTTCCATACCACCGAGTTGCCGGCCACCAGGGCCAGGGCGGTGTTCCAGGCCCACACGGCAACCGGGAAGTTGAAGGCGCTGATCACGCCGACCACACCCAGc                                                                                                        <  2:108966/139‑1 (MQ=255)
                                                        gCAGGCCAGGGCGGTCAGCGGAGTCTTCTCGGACGGTTTCCATACCACCGAGTTGCCGGCCACCAGGGCCAGGGCGGTGTTCCAGGCCCACACGGCAACCGGGAAGTTGAAGGCGCTGATCACGCCGACCACACCCAGCgg                                                                                                      <  7:188981/141‑1 (MQ=255)
                                                        gCAGGCCAGGGCGGTCAGCGGAGTCTTCTCGGACGGTTTCCATACCACCGAGTTGCCGGCCACCAGGGCCAGGGCGGTGTTCCAGGCCCACACGGCAACCGGGAAGTTGAAGGCGCTGATCACGCCGACCACACCCAGCgg                                                                                                      >  8:188981/1‑141 (MQ=255)
                                                         cAGGCCAGGGCGGTCAGCGGAGTCTTCTCGGACGGTTTCCATACCACCGAGTTGCCGGCCACCAGGGCCAGGGCGGTGTTCCAGGCCCACACGGCAACCGGGAAGTTGAAGGCGCTGATCACGCCGACCACACCCAGCGGGTGCCAGGt                                                                                             <  5:299919/149‑1 (MQ=255)
                                                          aGGCCAGGGCGGTCAGCGGAGTCTTCTCGGACGGTTTCCATACCACCGAGTTGCCGGCCACCAGGGCCAGGGCGGTGTTCCAGGCCCACACGGCAACCGGGAAGTTGAAGGCGCTGATCACGCCGACCACACCCAGCGGGTGCCAGGtt                                                                                            <  5:330788/149‑1 (MQ=255)
                                                             ccAGGGCGGTCAGCGGAGTCTTCTCGGACGGTTTCCATACCACCGAGTTGCCGGCCACCAGGGCCAGGGCGGTGTTCCAGGCCCACACGGCAACCGGGAAGTTGAAGGCGCTGATCACGCCGACCACACCCAGCGGGTGCCAGGTTTCa                                                                                         <  3:254405/149‑1 (MQ=255)
                                                                  gCGGTCAGCGGAGTCTTCTCGGACGGTTTCCATACCACCGAGTTGCCGGCCACCAGGGCCAGGGCGGTGTTCCAGGCCCACACGGCAACCGGGAAGTTGAAGGCGCTGATCACGCCGACCACACCCAGCGGGTGCCAGGTTTCACGCAt                                                                                    <  7:153761/149‑1 (MQ=255)
                                                                     gTCAGCGGAGTCTTCTCGGACGGTTTCCATACCACCGAGTTGCCGGCCACCAGGGCCAGGGCGGTGTTCCAGGCCCACACGGCAACCGGGAAGTTGAAGGCGCTGATCACGCCGACCACACCCAGCGGGTGCCAGGTTTCACGCAtgtg                                                                                 <  3:128235/149‑1 (MQ=255)
                                                                        aGCGGAGTCTTCTCGGACGGTTTCCATACCACCGAGTTGCCGGCCACCAGGGCCAGGGCGGTGTTCCAGGCCCACACGGCAACCGGGAAGTTGAAGGCGCTGATCACGCCGACCACACCCAGCGGGTGCCAGGTTTCACGCATgtggtg                                                                              >  2:291377/1‑149 (MQ=255)
                                                                             aGTCTTCTCGGACGGTTTCCATACCACCGAGTTGCCGGCCACCAGGGCCAGGGCGGTGTTCCAGGCCCACACGGCAACCGGGAAGTTGAAGGCGCTGATCACGCCGACCACACCCAGCGGGTGCCAGGTTTCACGCATGTGGTGGCCCg                                                                         <  8:22078/149‑1 (MQ=255)
                                                                              gTCTTCTCGGACGGTTTCCATACCACCGAGTTGCCGGCCACCAGGGCCAGGGCGGTGTTCCAGGCCCACACGGCAACCGGGAAGTTGAAGGCGCTGATCACGCCGACCACACCCAGCGGGTGCCAGGTTTCACGCATGTGGTGGCCCgg                                                                        <  8:152773/149‑1 (MQ=255)
                                                                                      ggACGGTTTCCATACCACCGAGTTGCCGGCCACCAGGTCCAGGGCGGTGTTCCAGGCCCACACGGCAACCGGGAAGTTGAAGGCGCTGATCACGCCGACCACACCCAGCGGGTGCCAGGTTTCACGCATGTGGTGGCCCGGGCGCTc                                                                  <  2:358480/147‑1 (MQ=255)
                                                                                      ggACGGTTTCCATACCACCGAGTTGCCGGCCACCAGGGCCAGGGCGGTGTTCCAGGCCCACATGGCAACCGGGAAGTTGAAGGCGCTGATCACGCCGACCACACCCAGCGGGTGCCAGGTTTCACGCATGTGGTGGCCCGGGCGCTc                                                                  <  8:172906/147‑1 (MQ=255)
                                                                                      ggACGGTTTCCATACCACCGAGTTGCCGGCCACCAGGGCCAGGGCGGTGTTCCAGGCCCACACGGCAACCGGGAAGTTGAAGGCGCTGATCACGCCGACCACACCCAGCGGGTGCCAGGTTTCACGCATGTGGTGGCCCGGGCGCTc                                                                  <  6:133455/147‑1 (MQ=255)
                                                                                      ggACGGTTTCCATACCACCGAGTTGCCGGCCACCAGGGCCAGGGCGGTGTTCCAGGCCCACACGGCAACCGCGAAGTTGAAGGCGCTGATCACGCAGACCACACCCAGCGGGTGCCAGGTATCACGCATGTGGTGGCCCGGGCGCTct                                                                 >  8:239670/1‑147 (MQ=255)
                                                                                      ggACGGTCTCCATACCACCGAGTTGCCGGCCACCAGGGCCAGGGCGGTGTTCCAGGCCCACACGGCAACCGGGAAGTTGAAGGCGCTGATCACGCCGACCACACCCAGCGGGTGCCAGGTTTCAGGCATGTGGTGGCCCGGGCGCTCgg                                                                <  3:212177/149‑1 (MQ=255)
                                                                                        aCGGTTTCCATACCACCGAGTTGCCGGCCACCAGGGCCAGGGCGGTGTTCCAGGCCCACACGGCAACCGGGAAGTTGAAGGCGCTGATCACGCCGACCACACCCAGCGGGTGCCAGGTTTCACGCATGTGGTGGCCCGGGCGCTCGGaa                                                              <  5:13027/149‑1 (MQ=255)
                                                                                            tttCCATACCACCGAGTTGCCGGCCACCAGGGCCAGGGCGGTGTTCCAGGCCCACACGGCAACCGGGAAGTTGAAGGCGCTGATCACGCCGACCACACCCAGCGGGTGCCAGGTTTCACGCATGTGGTGGCCCGGGCGCTCGGAAGCGa                                                          >  3:194956/1‑149 (MQ=255)
                                                                                             ttCGATAGCACCGAGTTGCAGGACACCAGGGACAGGGCGGTGTTCCAGGCCCACACGTCAACCGGGAAGTTGAAGTCGCTGATCACGCCGACCAGTCCCAGCGTGTGCCAGGTTTCACGCATGTGGTGGCCCGGGCGCTCGGAAGCGa                                                          <  2:233374/148‑1 (MQ=255)
                                                                                              tCCATACCACCGAGTTGCCGGCCACCAGGGCCAGGGCGGTGTTCCAGGCCCACACGGCAACCGGGAAGTTGAAGGCGCTGATCACGCCGACCACACCCAGCGGGTGCCAGGTTTCACGCATGTGGTGGCCCGGGCGCTc                                                                  >  3:174289/1‑139 (MQ=255)
                                                                                              tCCATACCACCGAGTTGCCGGCCACCAGGGCCAGGGCGGTGTTCCAGGCCCACACGGCAACCGGGAAGTTGAAGGCGCTGATCACGCCGACCACACCCAGCGGGTGCCAGGTTTCACGCATGTGGTGGCCCGGGCGCTc                                                                  <  4:174289/139‑1 (MQ=255)
                                                                                               ccATACCACCGAGTTGCCGGCCACCAGGGCCAGGGCGGTGTTCCAGGCCCACACGGCAACCGGGAAGTTGAAGGCGCTGATCACGCCGACCACACCCAGCGGGt                                                                                                    <  1:316611/104‑1 (MQ=255)
                                                                                               ccATACCACCGAGTTGCCGGCCACCAGGGCCAGGGCGGTGTTCCAGGCCCACACGGCAACCGGGAAGTTGAAGGCGCTGATCACGCCGACCACACCCAGCGGGt                                                                                                    >  2:316611/1‑104 (MQ=255)
                                                                                                 aTACCACCGAGTTGCCGGCCACCAGGGCCAGGGCGGTGTTCCAGGCCCACACGGCAACCGGGAAGTTGAAGGCGCTGATCACGCCGACCACACCCAGCGGGTGCCAGGTTTCACGCATGTGGTGGCCCgg                                                                        <  3:295112/130‑1 (MQ=255)
                                                                                                 aTACCACCGAGTTGCCGGCCACCAGGGCCAGGGCGGTGTTCCAGGCCCACACGGCAACCGGGAAGTTGAAGGCGCTGATCACGCCGACCACACCCAGCGGGTGCCAGGTTTCACGCATGTGGTGGCCCgg                                                                        >  4:295112/1‑130 (MQ=255)
                                                                                                     caccGAGTTGCCGGCCACCAGGGCCAGGGCGGTGTTCCAGGCCCACACGGCAACCGGGAAGTTGAAGGCGCTg                                                                                                                             <  5:347508/73‑1 (MQ=255)
                                                                                                     caccGAGTTGCCGGCCACCAGGGCCAGGGCGGTGTTCCAGGCCCACACGGCAACCGGGAAGTTGAAGGCGCTg                                                                                                                             >  6:347508/1‑73 (MQ=255)
                                                                                                     caccGAGTTGCCGGCCACCAGGGCCAGGGCGGTGTTCCAGGCCCACACGGCAACCGGGAAGTTGAAGGCGCTGATCACGCCGACCACACCCAGCGGGTGCCAGGTTTCACGCATGTGGTGGCCCGGGCGCTCGGAAGCGATGGTCAGGc                                                 >  1:20551/1‑149 (MQ=255)
                                                                                                     caccGAGTTGCCGGCCACCAGGGCCAGGGCGGTGTTCCAGGCCCACACGGCAACCGGGAAGTTGAAGGCGCTGATCACGCCGACCACACCCAGCGGGTGCCAGGTTTCACGCATGTGGTGGCCCGGGCGCTCGGAAGCGATGGTCAGGc                                                 >  5:36357/1‑149 (MQ=255)
                                                                                                              gCCGGCCACCAGGGCCAGGGCGGTGTTCCAGGCCCACACGGCAACCGGGAAGTTGAAGGCGCTGATCACGCCGACCACACCCAGCGGGTGCCAGGTTTCACGCATGTGGTGGCCCGGGCGCTCGGAAGCGATGGTCAGGCCGTACAGc                                         <  4:184761/148‑1 (MQ=255)
                                                                                                               ccGGCCACCAGGGCCAGGGCGGTGTTCCAGGCCCACACGGCAACCGGGAAGTTGAAGGCGCTGATCACGCCGACCACACCCAGCGGGTGCCAGGTTTCACGCATGTGGTGGCCCGGGCGCTCGGAAGCGATGGTCAGGCCGTACAGCTg                                       >  3:53707/1‑149 (MQ=255)
                                                                                                                cGGCCACCAGGGCCAGGGCGGTGTTCCAGGCCCACACGGCAACCGGGAAGTTGAAGGCGCTGATCACGCCGACCACACCCAGCGGGTGCCAGGTTTCACGCATGTGGTGGCCCGGGCGCTCGGAAGCGATGGTCAGGCCGTACAGCTgg                                      >  1:267601/1‑149 (MQ=255)
                                                                                                                   ccaccaGGGCCAGGGCGGTGTTCCAGGCCCACACGGCAACCGGGAAGTTGAAGGCGCTGATCACGCCGACCACACCCAGCGGGTGCCAGGTTTCACGCATGTGGTGGCCCGGGCGCTCCGAAGCGATGGTCAGGCCGTACAGCTGgcgc                                   <  1:30813/149‑1 (MQ=255)
                                                                                                                      ccagggccagggCGGTGTTCCAGGCCCACACGGCAACCGGGAAGTTGAAGGCGCTGATCACGCCGACCACACCCAGCGGGTGCCAGGTTTCACGCATGTGGTGGCCCGGGCGCTCGGAAGCGATGGTCAGGCCGTACAGCTGGCGCGa                                 >  1:242375/1‑148 (MQ=255)
                                                                                                                          ggccagggcGGTGTTCCAGGCCCACACGGCAACCGGGAAGTTGAAGGCGCTGATCACGCCGACCACACCCAGCGGGTGCCAGGTTTCACGCATGTGGTGGCCCGGGCGCTCGGAAGCGATGGTCAGGCCGTACAGCTGGCGCGACAGGc                            <  2:267601/149‑1 (MQ=255)
                                                                                                                             cagggcGGTGTTCCAGGCCCACACGGCAACCGGGAAGTTGAAGGCGCTGATCACGCCGACCACACCCAGCGGGTGCCAGGTTTCACGCATGTGGTGGCCCGGGCGCTCGGAAGCGATGGTCAGGCCGTAc                                            <  4:276057/130‑1 (MQ=255)
                                                                                                                             cagggcGGTGTTCCAGGCCCACACGGCAACCGGGAAGTTGAAGGCGCTGATCACGCCGACCACACCCAGCGGGTGCCAGGTTTCACGCATGTGGTGGCCCGGGCGCTCGGAAGCGATGGTCAGGCCGTAc                                            >  3:276057/1‑130 (MQ=255)
                                                                                                                               gggcGGTGTTCCAGGCCCACATGGCAACCGGGAAGTTGAAGGCGCTGATCACGCCGACCACACCCAGCGGGTGCCAGGTTTCACGCATGTGGTGGCCCGGGCGCTCGGAAGCGATGGTCAGGCCGTACAGCTGGCGCGACAGGCCAACg                       <  6:125526/149‑1 (MQ=255)
                                                                                                                               gggcGGTGTTCCAGGCCCACACGGCAACCGGGAAGTTGAAGGCGCTGATCACGCCGACCACACCCAGCGGGTGCCAGGTTTCACGCATGTGGTGGCCCGGGCGCTCGGAAGCGATGGTCAGGCCGTACAGCTGGCGCGACAGGCCAACg                       <  1:291377/149‑1 (MQ=255)
                                                                                                                                       ttCCAGGCCCACACGGCAACCGGGAAGTTGAAGGCGCTGATCACGCCGACCACACCCAGCGGGTGCCAGGTTTCACGCATGTGGTGGCCCGGGCGCTCGGAAGGGATGGTCAGGCCGTACAGGTGGCGCGACAGGCCAACGGCGAAGTc               <  2:20551/149‑1 (MQ=255)
                                                                                                                                         ccAGGCCCACACGGCAACCGGGAAGTTGAAGGCGCTGATCACGCCGACCACACCCAGCGGGTGCCAGGTTTCACGCATGTGGTGGCCCGGGCGCTCGGAAGCGATGGTCAGGCCGTACAGCTGGCGCGACAGGCCAACGGc                     <  6:274339/141‑1 (MQ=255)
                                                                                                                                         ccAGGCCCACACGGCAACCGGGAAGTTGAAGGCGCTGATCACGCCGACCACACCCAGCGGGTGCCAGGTTTCACGCATGTGGTGGCCCGGGCGCTCGGAAGCGATGGTCAGGCCGTACAGCTGGCGCGACAGGCCAACGGCGAAGTCg              <  4:199433/148‑1 (MQ=255)
                                                                                                                                         ccAGGCCCACACGGCAACCGGGAAGTTGAAGGCGCTGATCACGCCGACCACACCCAGCGGGTGCCAGGTTTCACGCATGTGGTGGCCCGGGCGCTCGCAAGCGATGGTCAGGCCGTACAGCTGGCGCGACAGGCCAACGGc                     >  5:274339/1‑141 (MQ=255)
                                                                                                                                          cAGGCCCACACGGCAACCGGGAAGTTGAAGGCGCTGATCACGCCGACCACACCCAGCGGGTGCCAGGTTTCACGCATGTGGTGGCCCGGGCGCTCGGAAGCGATGGTTAGGCCGTACAGCTGGCGCGACAGGCCAACGGCGAAGTCg              >  8:188470/1‑147 (MQ=255)
                                                                                                                                                 acacGGCAACCGGGAAGTTGAAGGCGCTGATCACGCCGACCACACCCAGCGGGTGCCAGGTTTCACGCATGTGGTGGCCCGGGCGCTCGGAAGCGATGGTCAGGCCGTACAGCTGGCGCGACAGGCCAACGGCGAAGTCGCAGATGTc      >  4:12892/1‑148 (MQ=255)
                                                                                                                                                  cacGGCAACCGGGAAGTTGAAGGCGCTGATCACGCCGACCACACCCAGCGGGTGCCAGGTTTCACGCATGTGGTGGCCCGGGCGCTCGGAAGCGATGGTCAGGCCGTACAGCTGGCGCGACAGGCCAACGGCGAAGTCGCAGATGTCGa    <  4:53707/149‑1 (MQ=255)
                                                                                                                                                    cGGCAACCGGGAAGTTGAAGGCGCTGATCACGCCGACCACACCCAGCGGGTGCCAGGTTTCACGCATGTGGTGGCCCGGGCGCTCGGAAGCGATGGTCAGGCCGTACAGCTGGCGCGACAGGCCAACGGCGAAGTCGCAGATGTCGATc  <  1:19129/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
GCCAAGCCAGCCGGAGCATCGCCGAAGGCTTTCAGGGCTTTTTCGAACAACGCCTGGCAGGCCAGGGCGGTCAGCGGAGTCTTCTCGGACGGTTTCCATACCACCGAGTTGCCGGCCACCAGGGCCAGGGCGGTGTTCCAGGCCCACACGGCAACCGGGAAGTTGAAGGCGCTGATCACGCCGACCACACCCAGCGGGTGCCAGGTTTCACGCATGTGGTGGCCCGGGCGCTCGGAAGCGATGGTCAGGCCGTACAGCTGGCGCGACAGGCCAACGGCGAAGTCGCAGATGTCGATC  >  NC_002947/6003326‑6003622

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑