Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 6,065,215 C→T 6.5% A39V (GCC→GTC)  phoB → two‑component system DNA‑binding response regulator PhoB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029476,065,2150CT 6.5% 176.8 / 2.0 61A39V (GCC→GTC) phoBtwo‑component system DNA‑binding response regulator PhoB
Reads supporting (aligned to +/- strand):  ref base C (29/28);  new base T (2/2);  total (31/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.14e-01

TGTCACACAGCTGCAATAATTCCGTTATCTAATGCGGGCCAAGACACCGAATCCAGGAGAGGTTTTGAGCATGGTAGGCAGGAACATTCTGATCGTCGACGACGAAGCGCCCATTCGCGAGATGATCGCCGTTGCCCTGGAAATGGCCGGCTATGACTGCCTGGAAGCGGAAAACTCCCAACAGGCCCACGCGATCATCGTCGACCGCAAACCGGACCTGATCCTGCTCGACTGGATGCTGCCGGGCACCTCCGGCATCGAGCTGGCCCGCCGCCTGAAGCGCGACGAACT  >  NC_002947/6065069‑6065359
                                                                                                                                                  |                                                                                                                                                
tGTCACACAGCTGCAATAATTCCGTTATCTAATGCGGGCCAAGACACCGAATCCAGGAGAGGTTTTGAGCATGGTAGGCAGGAACATTCTGATCGTCGACGACGAAGCGCCCATTCGCGAGATGATCGCCGTTGCCCTGGAAATGGCCg                                                                                                                                                >  4:320128/1‑149 (MQ=255)
    acacaGCTGCAATAATTCCGTTATCTAATGCGGGCCAAGACACCGAATCCAGGAGAGGTTTTGAGCATGGTAGGCAGGAACATTCTGATCGTCGACGACGAAGCGCCCATTCGCGAGATGATCGCCGTTGCCCTGGAAATGGCCGGCt                                                                                                                                             <  4:254363/148‑1 (MQ=255)
     cacaGCTGCAATAATTCCGTTATCTAATGCGGGCCAAGACACCGAATCCAGGAGAGGTTTTGAGCATGGTAGGCAGGAACATTCTGATCGTCGACGACGAAGCGCCCATTCGCGAGATGATCGCCGTTGCCCTGGAAATGGCCGGCTAt                                                                                                                                           <  5:289143/149‑1 (MQ=255)
      acaGCTGCAATAATTCCGTTATCTAATGCGGGCCAAGACACCGAATCCAGGAGAGGTTTTGAGCATGGTAGGCAGGAACATTCTGATCGTCGACGACGAAGCGCCCATTCGCGAGATGATCGCCGTTGCCCTGGAAATGGCCGGCTAt                                                                                                                                           >  1:18552/1‑148 (MQ=255)
      acaGCTGCAATAATTCCGTTATCTAATGCGGGCCAAGACACCGAATCCAGGAGAGGTTTTGAGCATGGTAGGCAGGAACATTCTGATCGTCGACGACGAAGCGCCCATTCGCGAGATGATCGCCGTTGCCCTGGAAATGGCCGGCTAt                                                                                                                                           >  3:348875/1‑148 (MQ=255)
      acaGCTGCAATAATTCCGTTATCTAATGCGGGCCAAGACACCGAATCCAGGAGAGGTTTTGAGCATGGTAGGCAGGAACATTCTGATCGTCGACGACGAAGCGCCCATTCGCGAGATGATCGCCGTTGCCCTGGAAATGGCCGGCTAt                                                                                                                                           <  1:220111/148‑1 (MQ=255)
      acaGCTGCAATAATTCCGTTATCTAATGCGGGCCAAGACACCGAATACAGGAGAGGTTTTGAGCATGGTAGGCAGGAACATTCTGATCGTCGACGACGAAGCGCCCATTCGCGAGATGATCGCCGTTGCCCTGGAAATGGCCGGCTAt                                                                                                                                           >  2:220111/1‑148 (MQ=255)
        aGCTGCAATAATTCCGTTATCTAATGCGGGCCAAGACACCGAATCCAGGAGAGGTTTTGAGCATGGTAGGCAGGAACATTCTGATCGTCGACGACGAAGCGCCCATTCGCGAGATGATCGCCGTTGCCCTGGAAATGGCCGGCTATGAc                                                                                                                                        >  5:12899/1‑149 (MQ=255)
             cAATAATTCCGTTATCTAATGCGGGCCAAGACACCGAATCCAGGAGAGGTTTTGAGCATGGTAGGCAGGAACATTATGATCGTCGACGACGAAGCGCCCATTAGCGAGATGATCGACGTTGACATGGAAATGGCCTGCTATGACTGCCt                                                                                                                                   >  7:339065/1‑149 (MQ=255)
                taatTCCGTTATCTAATGCGGGCCAAGACACCGAATCCAGGAGAGGTTTTGAGCATGGTAGGCAGGAACATTCTGATCGTCGACGACGAAGCGCCCATTCGCGAGATGATCGCCGTTGCCCTGGAAATGGCCGGCTATGACTGCCTgg                                                                                                                                 >  4:153889/1‑148 (MQ=255)
                  atTCCGTTATCTAATGCGGGCCAAGACACCGAATCCAGGAGAGGTTTTGAGCATGGTGGGCAGGAACATTCTGATCGTCGACGACGAAGCGCCCATTCGCGAGATGATCGCCGTTGCCCTGGAAATGGCCGGCTATGACTGCCTGGAAg                                                                                                                              <  2:107727/149‑1 (MQ=255)
                               aTGCGGGCCAAGACACCGAATCCAGGAGAGGTTTTGATCATGGTAGGCAGGAACATTCTGATCGGCGACGACGAAGCGCCCATTCGCGAGATGATCGCCGTTGCCCTGGAAATGGCCGGCTATGACTGCCTGGAAGCGGAAAACTccc                                                                                                                  <  2:349486/148‑1 (MQ=255)
                                          gACACCGAATCCAGGAGAGGTTTTGAGCATGGTAGGCAGGAACATTCTGATCGTCGACGACGAAGCGCCCATTCGCGAGATGATCGCCGTTGCCCTGGAAATGGCCGGCTATGACTGCCTGGAAGCGGAAAACTCCCAACAGGCCCAcg                                                                                                      >  4:355812/1‑149 (MQ=255)
                                          gACACCGAATCCAGGAGAGGTTTTGAGCATGGTAGGCAGGAACATTCTGATCGTCGACGACGAAGCGCCCATTCGCGAGATGATCGCCGTTGCCCTGGAAATGGCCGGCTATGACTGCCTGGAAGCGGAAAACTCCCAACAGGCCCAcg                                                                                                      <  3:153889/149‑1 (MQ=255)
                                             acCGAATCCAGGAGAGGTTTTGAGCATGGTAGGCAGGAACATTCTGATCGTCGACGACGAAGCGCCCATTCGCGAGATGATCGCCGTTGCCCTGGAAATGGCCGGCTATGACTGCCTGGAAGCGGAAAACTCCCAACAGGCCCAcgcg                                                                                                    >  4:186894/1‑148 (MQ=255)
                                               cGAATCCAGGAGAGGTTTTGAGCATGGTAGGCAGGAACATTCTGATCGTCGACGACGAAGCGCCCATTCGCGAGATGATCGCCGTTGCCCTGGAAATGGCCGGCTATGACTGCCTGGAAGCGGAAAACTCCCAACAGGCCCACGCGat                                                                                                  >  3:294570/1‑148 (MQ=255)
                                                gAATCCAGGAGAGGTTTTGAGCATGGTAGGCAGGAACATTCTGATCGTCGACGACGAAGCGCCCATTCGCGAGATGATCGCCGTTGCCCTGGAAATGGCCGGCTATGACTGCCTGGAAGCGGAAAACTCCCAACAGGCCCACGCGatca                                                                                                <  6:352839/149‑1 (MQ=255)
                                                 aaTCCAGGAGAGGTTTTGAGCATGGTAGGCAGGAACATTCTGATCGTCGACGACGAAGCGCCCATTCGCGAGATGATCGCCGTTGCCCTGGAAATGGCCGGCTATGACTGCCTGGAAGCGGAAAACTCCCAACAGGCCCACGCGatcat                                                                                               >  3:133200/1‑149 (MQ=255)
                                                             gTTTTGAGCATGGTAGGCAGGAACATTCTGATCGTCGACGACGAAGCGCCCATTCGCGAGATGATCGCCGTTGCCCTGGAAATGGCCGGCTATGACTGCCTGGAAGCGGAAAACTCCCAACAGGCCCACGCGATCATCGTCGACCGCa                                                                                    >  1:160375/1‑148 (MQ=255)
                                                                ttGAGCATGGTAGGCAGGAACATTCTGATCGTCGACGACGAAGCGCCCATTCGCGAGATGATCGCCGTTGCCCTGGAAATGGCCGGCTATGACTGCCTGGAAGCGGAAAACTCCCAACAGGCCCACGCGATCATCGTCGACCGCAAAc                                                                                 <  4:294570/148‑1 (MQ=255)
                                                                 tGAGCATGGTAGGCAGGAACATTCTGATCGTCGACGACGAAGCGCCCATTCGCGAGATGATCGCCGTTGCCCTGGAAATGGCCGGCTATGACTGCCTGGAAGCGGAAAACTCCCAACAGGCCCACGCGATCATCGTCGACCGCAAAcc                                                                                <  3:355812/148‑1 (MQ=255)
                                                                  gAGCATGGTAGGCAGGAACATTCTGATCGTCGACGACGAAGCGCCCATTCGCGAGATGATCGCCGTTGCGCTGGAAATGGCCGGCTATGACTGCCTGGAAGCGGAAAACTCCCAACAGGCCCACGCGATCATCGTCGACCGCAAACCgg                                                                              >  1:40008/1‑149 (MQ=255)
                                                                   aGCATGGTAGGCAGGAACATTCTGATCGTCGACGACGAAGCGCCCATTCGCGAGATGATCGCCGTTGCCCTGGAAATGGCCGGCTATGACTGCCTGGAAGCGGAAAACTCCCAACAGGCCCACGCGATCATCGTCGACCGCAAACCGGa                                                                             <  5:329825/149‑1 (MQ=255)
                                                                    gCATGGTAGGCAGGAACATTCTGATCGTCGACGACGAAGCGCCCATTCGCGAGATGATCGCCGTTGCCCTGGAAATGGCCGGCTATGACTGCCTGGAAGCGGAAAACTCCCAACAGGCCCACGCGATCATCGTCGACCGCAAACCGGAc                                                                            <  3:320128/149‑1 (MQ=255)
                                                                         gTAGTCAGGAACATTCTGATCGTCGACGACGAAGCGCCCATTCGCGAGATGATCGCCGTTGCCCTGGAAATGGCCGGCTATGACTGCCTGGAAGCGGAAAACTCCCAACAGGCCCAc                                                                                                       >  5:198575/1‑117 (MQ=255)
                                                                         gTAGGCAGGAACATTCTGATCGTCGACGACGAAGCGCCCATTCGCGAGATGATCGCCGTTGCCCTGGAAATGGCCGGCTATGACTGCCTGGAAGCGGAAAACTCCCAACAGGCCCAc                                                                                                       <  6:198575/117‑1 (MQ=255)
                                                                               aGGAACATTCTGATCGTCGACGACGAAGCGCCCATTCGCGAGATGATCGCCGTTGCCCTGGAAATGGCCGGCTATGACTGCCTGGAAGCGGAAAACTCACAACAGGACCACGCGATCATCGTCTACAGCAAACAGGACCTGATCCTTCt                                                                 >  1:359288/1‑149 (MQ=255)
                                                                               aGGAACATTCTGATCGTCGACGACGAAGCGCCAATTCGCGAGATGATCGCCGTTGCCCTGGAAATGGCAGGCTATGACTGCCTGGAAGCGGAAAACTCACAACAGGCCCACGCGATCATCGTCGACCGCAAACAGGACCTGATCCttc                                                                  >  4:161408/1‑146 (MQ=255)
                                                                                ggAACATTCTGATCGTCGACGACGAAGCGCCCATTCGCGAGATGATCGCCGTTGCCCTGGAAATGGCCGGCTATGACTGCCTGGAAGCGGAAAACTCCCAACAGGCCCACGCGATCATCGTCGACCGCAAACCGGACCTGATCCTGCTc                                                                <  3:186894/149‑1 (MQ=255)
                                                                                ggAACATTCTGATCGTCGACGACGAAGCGCCCATTCGCGAGATGATCGCCGTTGCCCTGGAAATGGCCGGCTATGACTGCCTGGAAGCGGAAAACTCCCAACAGGCCCACGCGATCATCGTCGACCGCAAACCGGACCTGATCCTGCTc                                                                <  7:283008/149‑1 (MQ=255)
                                                                                     aTTCTGATCGTCGACGACGAAGCGCCCATTCGCGAGATGATCGCCGTTGCCCTGGAAATGGCCGGCTATGACTGCCTGGAAGCGGAAAACTCCCAACAGGCCCACGCGATCATCGTCGACCGCAAACCGGACCTGATCCTGCTCGACTg                                                           >  1:333061/1‑149 (MQ=255)
                                                                                     aTTCTGATCGTCGACGACGAAGCGCCCATTCGCGAGATGATCGCCGTTGCCCTGGAAATGGCCGGCTATGACTGCCTGGAAGCGGAAAACTCCCAACAGGCCCACGCGATCATCGTCGACCGCAAACCGGACCTGATCCTGCTCGACTg                                                           >  1:338283/1‑149 (MQ=255)
                                                                                      ttCTGATCGTCGACGACGAAGCGCCCATTCGCGAGATGATCGCCGTTGCCCTGGAAATGGCCGGCTATGACTGCCTGGAAGCGGAAAACTCCCAACAGGCCCACGCGATCATCGTCGACCGCAAACCGGACCTGATCCTGCTCGACTgg                                                          <  4:133200/149‑1 (MQ=255)
                                                                                        cTGATCGTCGACGACGAAGCGCCCATTCGCGAGATGATCGCCGTTGCCCTGGAAATGGCCGGCTATGACTGCCTGGAAGCGGAAAACTCCCAACAGGCCCACGCGATCATCGTCGACCGCAAACCGGACCTGATCCTGCTCGACTGGa                                                         <  3:1647/148‑1 (MQ=255)
                                                                                        cTGATCGTCGACGACGAAGCGCCCATTCGCGAGATGATCGCCGTTGCCCTGGAAATGGCCGGCTATGACTGCCTGGAAGCGGAAAACTCCCAACAGGCCCACGCGATCATCGTCGACCGCAAACCGGACCTGATCCTGCTCGACTGGa                                                         <  2:160375/148‑1 (MQ=255)
                                                                                         tGATCGTCGACGACGAAGCGCCCATTCGCGAGATGATCGCCGTTGCCCTGGAAATGGCCGGCTATGACTGCCTGGAAGCGGAAAACTCCCAACAGGCCCACGCGATCATCGTCGACCGCAAACCGGACCTGATCCTGCTCGACTGGAtg                                                       >  6:80624/1‑149 (MQ=255)
                                                                                          gATCGTCGACGACGAAGCGCCCATTCGCGAGATGATCGCCGTTGCCCTGGAAATGGTCGGCTATGACTGCCTGGAAGCGGAAAACTCCCAACAGGCCCACGCGATCATCGTCGACCGCAAACCGGAc                                                                            >  8:226914/1‑127 (MQ=255)
                                                                                          gATCGTCGACGACGAAGCGCCCATTCGCGAGATGATCGCCGTTGCCCTGGAAATGGTCGGCTATGACTGCCTGGAAGCGGAAAACTCCCAACAGGCCCACGCAATCATCGTCGACCGCAAACCGGAc                                                                            <  7:226914/127‑1 (MQ=255)
                                                                                          gATCGTCGACGACGAAGCGCCCATTCGCGAGATGATCGCCGTTGCCCTGGAAATGGCCGGCTATGACTGCCTGGAAGCGGAAAACTCCCAACAGGCCCACGCGATCATCGTCGACCGCAAACCGGAc                                                                            >  8:84035/1‑127 (MQ=255)
                                                                                          gATCGTCGACGACGAAGCGCCCATTCGCGAGATGATCGCCGTTGCCCTGGAAATGGCCGGCTATGACTGCCTGGAAGCGGAAAACTCCCAACAGGCCCACGCGATCATCGTCGACCGCAAACCGGAc                                                                            <  7:84035/127‑1 (MQ=255)
                                                                                                 gacgacgaAGCGCCCATTCGCGAGATGATCGCCGTTGCCCTGGAAATGGCCGGCTATGACTGCCTGGAAGCGGAAAACTCCCAACAGGCCCACGCGATCATCGTCGACCGCAAACCGGACCTGATCCTGCTCGACTGGATGCTGCCggg                                               >  5:118256/1‑149 (MQ=255)
                                                                                                    gacgaAGCGCCCATTCGCGAGATGATCGCCGTTGCCCTGGAAATGGCCGGCTATGACTGCCTGGAAGCGGAAAACTCCCAACAGGCCCACGCGATCATCGTCGACCGCAAACCGGACCTGATCCTGCTCGACTGGATGCTGCCgg                                                <  2:252136/145‑1 (MQ=255)
                                                                                                    gacgaAGCGCCCATTCGCGAGATGATCGCCGTTGCCCTGGAAATGGCCGGCTATGACTGCCTGGAAGCGGAAAACTCCCAACAGGCCCACGCGATCATCGTCGACCGCAAACCGGACCTGATCCTGCTCGACTGGATGCTGCCgg                                                >  1:252136/1‑145 (MQ=255)
                                                                                                       gaAGCGCCCATTCGCGAGATGATCGCCGTTGCCCTGGAAATGGCCGGCTATGACTGCCTGGAAGCGGAAAACTCCCAACAGGCCCACGCGATCATCGTCGACCGCAAACCGGACCTGATCCt                                                                    <  7:204619/122‑1 (MQ=255)
                                                                                                       gaAGCGCCCATTCGCGAGATGATCGCCGTTGCCCTGGAAATGGCCGGCTATGACTGCCTGGAAGCGGAAAACTCCCAACAGGCCCACGCGATCATCGTCGACCGCAAACCGGACCTGATCCt                                                                    >  8:204619/1‑122 (MQ=255)
                                                                                                          gcgcCCATTCGCGAGATGATCGCCGTTGCCCTGGAAATGGCCGGCTATGACTGCCTGGAAGCGGAAAACTCCCAACAGGCCCACGCGATCATCGTCGACCGCAAACCGGACCTGATCCTGCTCGACTGGATGCTGCCGGGCACCTCCgg                                      >  5:72825/1‑149 (MQ=255)
                                                                                                            gcCCATTCGCGAGATGATCGCCGTTGCCCTGGAAATGGCCGGCTATGACTGCCTGGAAGCGGAAAACTCCCAACAGGCCCACGCGATCATCGTCGACCGCAAACCGGACCTGATCCTGCTCGACTGGATGCTGCCGGGCACCTCCGGc                                     >  4:202629/1‑148 (MQ=255)
                                                                                                             cccATTCGCGAGATGATCGCCGTTGCCCTGGAAATGGTCGGCTATGACTGCCTGGAAGCGGAAAACTCCCAACAGGCCCACGCGATCATCGTCGACCGCAAACCGGACCTGATCCTGCTCGACTGGATGCTGCCGGGCACCTCCGGCAt                                   >  6:171237/1‑149 (MQ=255)
                                                                                                                        gatgatCGCCGTTGCCCTGGAAATGGCCGGCTATGACTGCCTGGAAGCGGAAAACTCCCAACAGGCCCACGCGATCATCGTCGACCGCAAACCGGACCTGATCCTGCTCGACTGGATGCTGCCGGGCACCTCCGGCATCGAGCTGGccc                        <  2:18552/149‑1 (MQ=255)
                                                                                                                         atgatCGCCGTTGCCCTGGAAATGGCCGGCTATGACTGCCTGGAAGCGGAAAACTCCCAACAGGCCCACGCGATCATCGTCGACCGCAAACCGGACCTGATCCTGCTCGACTGGATGCTGCCGGGCACCTCCGGCATCGAGCTGGCccg                       >  6:181149/1‑149 (MQ=255)
                                                                                                                             tCGCCGTTGCCCTGGAAATGGCCGGCTATGACTGCGTGGACGCGGAAAACTCCCAACAGGCCCACGCGATCATCGTCGCCCGCAAACCGGACCTGATCCTGCTCGACTGGATGCTGCCGGGCACCTCCGGCATCGAGCTGGCCcgccgc                   >  6:226741/1‑149 (MQ=255)
                                                                                                                                   ttGCCCTGGAAATGGCCGGCTATGACTGCCTGGAAGCGGAAAACTCCCAACAGGCCCACGCGATCATCGTCGACCGCAAACCGGACCTGATCCTGCTCGACTGGATGCTGCCGGGCACCTCCGGCATCGAGCTGGCCCGCCGCCTGAAg             <  8:153069/149‑1 (MQ=255)
                                                                                                                                     gCCCTGGAAATGGCCGGCTATGACTGCCTGGAAGCGGAAAACTCCCAACAGGCCCACGCGATCATCGTCGAcc                                                                                       >  8:342778/1‑73 (MQ=255)
                                                                                                                                     gCCCTGGAAATGGCCGGCTATGACTGCCTGGAAGCGGAAAACTCCCAACAGGCCCACGCGATCATCGTCGAcc                                                                                       <  7:342778/73‑1 (MQ=255)
                                                                                                                                     gCCCTGGAAATGGCCGGCTATGACTGCCTGGAAGCGGAAAACTCCCAACAGGCCCACGCGATCATCGTCGACCGCAAACCGGAGCTGATCCTGCTCGACTGGATGCTGCCGGGCACCTCCGGCATCGAGCTGGCCCGCCGCCTGAAgc            <  6:118256/148‑1 (MQ=255)
                                                                                                                                      cccTGGAAATGGTCGGCTATGACTGCCTGGAAGCGGAAAACTCCCAACAGGCCCACGCGATCATCGTCGACCGCAAACCGGACCTGATCCTGCTCGACTGGATGCTGCCGGGCACCTCCGGCATCGAGCTGGCCCGCCGCCTGAAgcgc          <  5:171237/149‑1 (MQ=255)
                                                                                                                                        cTGGAAATGGCCGGCTATGACTGCCTGGAAGCGGAAAACTCCCAACAGGCCCACGCGATCATCGTCGACCGCAAACCGGACCTGATCCTGCTCGACTGGATGCTGCCGGGCACCTCCGGCATCGAGCTGGCCCGCCGCCTGAAGCGcga        >  3:178022/1‑149 (MQ=255)
                                                                                                                                        cTGGAAATGGCCGGCTATGACTGCCTGGAAGCGGAAAACTCCCAACAGGCCCACGCGATCATCGTCGACCGCAAACCGGACCTGATCCTGCTCGACTGGATGCTGCCGGGCACCTCCGGCATCGAGCTGGCCCGCCGCCTGAAGCGcga        <  2:40008/149‑1 (MQ=255)
                                                                                                                                             taTGGCCGGCTATGACTGCCTGGAAGCGGAAAACTCCCAACAGGCCCACGAGATCATCGTCGACCGCAAACCTGACCTGATCCTGCTCGACTGGATGCTGCCGGGCACCTCCGGCATCGAGCTGGCCCGCCGCCTGAAGCGCGACGAAc   <  8:339065/148‑1 (MQ=255)
                                                                                                                                             aaTGGCCGGCTATGACTGCCTGGAAGCTGAAAACTCCCAACAGGCCCACGCGATCATCGTCGACCGCAAACCGGACCTGATCCTGCTCGACTGGATGCTGCCGGGCACCTCCGGCATCGAGCTGGCCCGCCGCCTGAAGCGCGACGaa    <  2:359288/148‑1 (MQ=255)
                                                                                                                                              aTGGCCGGCTATGACTGCCTGGAAGCGGAAAACTCCCAACAGGCCCACGCGATCATCGTCGACCGCAAACCGGACCTGATCCTGCTCGACTGGATGCTGCCGGGCACCTCCGGCATCGAGCTGGCCCGCCGCCTGAAGCGCGACGAACt  <  6:72825/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                
TGTCACACAGCTGCAATAATTCCGTTATCTAATGCGGGCCAAGACACCGAATCCAGGAGAGGTTTTGAGCATGGTAGGCAGGAACATTCTGATCGTCGACGACGAAGCGCCCATTCGCGAGATGATCGCCGTTGCCCTGGAAATGGCCGGCTATGACTGCCTGGAAGCGGAAAACTCCCAACAGGCCCACGCGATCATCGTCGACCGCAAACCGGACCTGATCCTGCTCGACTGGATGCTGCCGGGCACCTCCGGCATCGAGCTGGCCCGCCGCCTGAAGCGCGACGAACT  >  NC_002947/6065069‑6065359

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑