Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 6,141,001 T→C 8.0% I246T (ATC→ACC)  cusA → CzcA family RND family copper transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029476,141,0010TC 8.0% 141.2 / 2.8 50I246T (ATC→ACC) cusACzcA family RND family copper transporter
Reads supporting (aligned to +/- strand):  ref base T (23/23);  new base C (2/2);  total (25/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.57e-01

CCAGCCTCGGCATCACCCAGGGCGAGGTCGCAAACGCCATCGGTAAGGCCAATCAGGAAACAGGCGGTGGCGTGCTGGAACAGGGCGAGGCGGAGTTCATGGTGCGGGCCTCGGGCTACCTGAAGACACTGGACGACTTTCGCGCCATCCCCCTGCGCCTGGCTGCCAACGGTGCACCGGTGATGCTGGGCGACGTTGCCAGAGTACAGCTCGGACCGGAAGCCCGACGGGGTATCGGAGAGCTGGACGGCGAAGGCGAAGCCGTGGGTGGTGTGGTGATCCTGCGCAGTGGC  >  NC_002947/6140854‑6141146
                                                                                                                                                   |                                                                                                                                                 
ccAGCCTCGGCATCACCCAGGGCGAGGTCGCAAACGCCATCGGTAAGGCCAATCAGGAAACAGGCGGTGGCGTGCTGGAACAGGGCGAGGCGGAGTTCATGGTGCGGGCCTCGGGCTACCTGAAGACACTGGACGACTTTCGCGCCATc                                                                                                                                                  <  1:204043/149‑1 (MQ=255)
     cTCGGCATCACCCAGGGCGAGGTCGCAAACGCCATCGGTAAGGCCAATCAGGAAACAGGCGGTGGCGTGCTGGAACAGGGCGAGGCGGAGTTCATGGTGCGGGCCTCGGGCTACCTGAAGACACTGGACGACTTTCGCGCCATCCCCCt                                                                                                                                             <  1:199679/149‑1 (MQ=255)
              aCCCAGGGCGAGGTCGCAAACGCCATCGGTAAGGCCAATCAGGAAACAGGCGGTGGCGTGCTGGAACAGGGCGAGGCGGAGTTCATGGTGCGGGCCTCGGGCTACCTGAAGACACTGGACGACTTTCGCGCCATCCCCCTGCGCCTGGc                                                                                                                                    >  3:112020/1‑149 (MQ=255)
               ccgagggcgagggcgCAAACGGCATCGGTAAGGCCAATCAGGACTCCGGCGGTGGCGTGCTGGAACAGGGCGAGGCGGAGTTCATGGTGCGGGCGGCGGGCTACCTGAAGACACTGGACGACTTTCGCGCCATCCCCCTGCGCCTGGCt                                                                                                                                   <  3:267435/146‑1 (MQ=255)
               cccAGGGCGAGGTCGCAAACGCCATCGGTAAGGCCAATCAGGAAACAGGCGGTGGCGTGCTGGAACAGGGCGAGGCGGAGTTCATGGTGCGGGCCTCGGGCTACCTGAAGACACTGGACGACTTTCGCGCCATCCCCCTGCGCCTGGCt                                                                                                                                   >  3:242131/1‑149 (MQ=255)
                             gCAAACGCCATCGGTAAGGCCAATCAGGAAACAGGCGGTGGCGTGCTGGAACAGGGCGAGGCGGAGTTCATGGTGCGGGCCTCGGGCTACCTGAAGACACTGGACGACTTTCGCGCCATCCCCCTGCGCCTGGCTGCCAACGGTGCAcc                                                                                                                     >  8:230827/1‑149 (MQ=255)
                              cAAACGCCATCGGTAAGGCCAATCAGGAAACAGGCGGTGGCGTGCTGGAACAGGGCGAGGCGGAGTTCATGGTGCGGGCCTCGGGCTACCTGAAGACACTGGACGACTTTCGCGCCATCCCCCTGCGCCTGGCTGCCAACGGTGCACCg                                                                                                                    <  7:61875/149‑1 (MQ=255)
                              cAAACGCCATCGGTAAGGCCAATCAGGAAACAGGCGGTGGCGTGCTGGAACAGGGCGAGGCGGAGTTCATGGTGCGGGCCTCGGGCTACCTGAAGACACTGGACGACTTTCGCGCCATCCCCCTGCGCCTGGCTGCCAACGGTGCACCg                                                                                                                    >  1:265595/1‑149 (MQ=255)
                              cAAACGACATCGGTAAGGCCAATCAGGAAACAGGCGGTGGCGTGCTGGAACAGGGCGAGGCGGAGTTCATGGTGCGGTCCTCGGGCTACCTGAAGACACTGTACGACTTTCGCGCCATCCACCTGCGCCTGTCTGCCAACGGTGAACCg                                                                                                                    >  6:299979/1‑149 (MQ=255)
                               aaaCGCCATCGGTAAGGCCAATCAGGAAACAGGCGGTGGCGTGCTGGAACAGGGCGAGGCGGAGTTCATGGTGCGGGCCTCGGGCTACCTGAAGACACTGGACGACTTTCGCGCCATCCCCCTGCGCCTGGCTGCCAACGGCGCACCgg                                                                                                                   >  1:314902/1‑149 (MQ=255)
                                   gCCATCGGTAAGGCCAATCAGGAAACAGGCGGTGGCGTGCTGGAACAGGGCGAGGCGGAGTTCATGGTGCGGGCCTCGGGCTACCTGAAGACACTGGACGACTTTCGCGCCATCCCCCTGCGCCTGGCTGCCAACGGTGCACCGGTGAt                                                                                                               >  1:195811/1‑149 (MQ=255)
                                         ggTAAGGCCAATCAGGAAACAGGCGGTGGCGTGCTGGAACAGGGCGAGGCGGAGTTCATGGTGCGGGCCTCGGGCTACCTGAAGACACTGGACGACTTTCGCGCCATCCCCCTGCGCCTGGCTGCCAACGGTGCACCGGTGATGCTggg                                                                                                         <  4:112020/149‑1 (MQ=255)
                                          gTAAGGCCAATCAGGAAACAGGCGGTGGCGTGCTGGAACAGGGCGAGGCGGAGTTCATGGTGTGGGCCTCGGGCTACCTGAAGACACTGGACGACTTTCGCGCCATCCCCCTGCGCCTGGCTGCCAACGGTGCACCGGTGATGCTGGGc                                                                                                        >  1:237114/1‑149 (MQ=255)
                                               gCCAATCAGGAAACAGGCGGTGGCGTGCTGGAACAGGGCGAGGCGGAGTTCATGGTGCGGGCCTCGGGCTACCTGAAGACACTGGACGACTTTCGCGCCATCCCCCTGCGCCTGGCTGCCAACGGTGCACCGGTGATGCTGGGCGACGt                                                                                                   >  4:95697/1‑149 (MQ=255)
                                                  aaTCAGGAAACAGGCGGTGGCGTGCTGGAACAGGGCGAGGCGGGGTTCATGGTGCGGGCCTCGGGCTACCTGAAGACACTGGACGACTTTCGCGCCATCCCCCTGCGCCTGGCTGCCAACGGt                                                                                                                          <  2:133217/123‑1 (MQ=255)
                                                  aaTCAGGAAACAGGCGGTGGCGTGCTGGAACAGGGCGAGGCGGGGTTCATGGTGCGGGCCTCGGGCTACCTGAAGACACTGGACGACTTTCGCGCCATCCCCCTGCGCCTGGCTGCCAACGGt                                                                                                                          >  1:133217/1‑123 (MQ=255)
                                                    tCAGGAAACAGGCGGTGGCGTGCTGGAACAGGGCGAGGCGGAGTTCATGGTGCGGGCCTCGGGCTACCTGAAGACACTGGACGACTTTCGCGCCATCCCCCTGCGCCTGGCTGCCAACGGTGCACCGGTGATGCTGGGCGACGTTGCCa                                                                                              <  2:195811/149‑1 (MQ=255)
                                                      aGGAAACAGGCGGTGGCGTGCTGGAACAGGGCGAGGCGGAGTTCATGGTGCGGGCCTCGGGCTACCTGAAGACACTGGACGACTTTCGCGCCATCCCCCTGCGCCTGGCTGCCAACGGTGCACCGGTGATGCTGGGCGACGTTGCCaga                                                                                            <  7:230827/149‑1 (MQ=255)
                                                      aGGAAACAGGCGGTGGCGTGCTGGAACAGGGCGAGGCGGAGTTCATGGTGCGGGCCTCGGGCTACCTGAAGACACTGGACGACTTTCGCGCCATCCCCCTGCGCCTGGCTGCCAACGGTGCACCGGTGATGCTGGGCGACGTTGCCaga                                                                                            <  5:240390/149‑1 (MQ=255)
                                                      aGGAAACAGGCGGTGGCGTGCTGGAACAGGGCGAGGCGGAGTTCATGGTGCGGGCCTCGGGCTACCTGAAGACACTGGACGACTTTCGCGCCATCCCCCTGCGCCTGGCTGCCAACGGTGCACCGGTGATGCTGGGCGACGTTGCCaga                                                                                            <  3:254994/149‑1 (MQ=255)
                                                       ggAAACAGGCGGTGGCGTGCTGGAACAGGGCGAGGCGGAGTTCATGGTGCGGGCCTCGGGCTACCTGAAGACACTGGACGACTTTCGCGCCATCCCCCTGCGCCTGGCTGCCAACGGTGCAc                                                                                                                      <  6:65174/122‑1 (MQ=255)
                                                       ggAAACAGGCGGTGGCGTGCTGGAACAGGGCGAGGCGGAGTTCATGGTGCGGGCCTCGGGCTACCTGAAGACACTGGACGACTTTCGCGCCATCCCCCTGCGCCTGGCTGCCAACGGTGCAc                                                                                                                      >  5:65174/1‑122 (MQ=255)
                                                         aaaCAGGCGGTGGCGTGCTGGAACAGGGCGAGGCGGAGTTCATGGTGCGGGCCTCGGGCTACCTGAAGACACTGGACGACTTTCGCGCCATCCCCCTGCGCCTGGCTGCCAACGGTGCACCGGTGATGCTGGGCGACGTTGCCAGAGTa                                                                                         <  4:242131/149‑1 (MQ=255)
                                                          aaCAGGCGGTGGCGTGCTGGAACAGGGCGAGGCGGAGTTCATGGTGCGGGCCTCGGGCTACCTGAAGACACTGGACGACTTTCGCGCCATCCCCCTGCGCCTGGCTGCCAACGGTGCACCGGTGATGCTGGGCGACGTTGCCAGAGTa                                                                                         <  2:265595/148‑1 (MQ=255)
                                                                             gAACAGGGCGAGGCGGAGTTCATGGTGCGGGCCTCGGGCTACCTGAAGACACTGGACGACTTTCGCGCCATCCCCCTGCGCCTGGCTGCCAACGGTGCACCGGTGATGCTGGGCGACGTTGCCaga                                                                                            >  6:29499/1‑126 (MQ=255)
                                                                             gAACAGGGCGAGGCGGAGTTCATGGTGCGGGCCTCGGGCTACCTGAAGACACTGGACGACTTTCGCGCCATCCCCCTGCGCCTGGCTGCCAACGGTGCACCGGTGATGCTGGGCGACGTTGCCaga                                                                                            <  5:29499/126‑1 (MQ=255)
                                                                             gAACAGGGCGAGGCGGAGTTCATGGTGCGGGCCTCGGGCTACCTGAAGACACTGGACGACTTTCGCGCCATCCCCCTGCGCCTGGCTGCCAACGGTGCACCGGTGATGCTGGGCGACGTTGCCAGAGTACAGCTCGGACCGGAAGccc                                                                      >  1:259311/1‑148 (MQ=255)
                                                                                          cGGAGTTCATGGTGCGGGCCTCGGGCTACCTGAAGACACTGGACGACTTTCGCGCCATCCCCCTGCGCCTGGCTGCCAACGGTGCACCGGTGATGCTGGGCGACGTTGCCAGAGTACAGCTCGGACCGGAAGCCCGACGGGGTATCGga                                                        <  2:259311/149‑1 (MQ=255)
                                                                                           ggAGTTCATGGTGTGGGCCTCGGGCTACCTGAAGACACTGGACGACTTTCGCGCCATCCCCCTGCGCCTGGCTGCCAACGGTGCACCGGTGATGCTGGGCGACGTTGCCAGAGTACAGCTCGGACCGGAAGCCCGACGGGGTATCGgag                                                       <  2:237114/149‑1 (MQ=255)
                                                                                              gTTCATGGTGCGGGCCTCGGGCTACCTGAAGACACTGGACGACTTTCGCGCCATCCCCCTGCGCCTGGCTGCCAACGGTGCACCGGTGATGCTGGGCGACATTGCCAGAGTACAGCTCGGACCGGAAGCCCGACGGGGTATCGGAGAGc                                                    >  5:356466/1‑149 (MQ=255)
                                                                                               ttCATGGTGCGGGCCTCGGGCTACCTGAAGACACTGGACGACTTTCGCGCCATCCCCCTGCGCCTGGCTGCCAACGGTGCACCGGTGATGCTGGGCGACGTTGCCAGAGTACAGCTCGGACCGGAAGCCCGACGGGGTATCGGAGAGCt                                                   >  8:279336/1‑149 (MQ=255)
                                                                                                  aTGGTGCGGGCCTCGGGCTACCTGAAGACACTGGACGACTTTCGCGCCATCCCCCTGCGCCTGGCTGCCAACGGTGCACCGGTGATGCTGGGCGACGTTGCCAGAGTACAGCTCGGACCGGAAGCCCGACGGGGTATCGGAGAGCTGGa                                                >  5:216865/1‑149 (MQ=255)
                                                                                                       gCGGGCCTCGGGCTACCTGAAGACACTGGACGACTTTCGCGCCATCCCCCTGCGCCTGGCTGCCAACGGTGCACCGGTGATGCTGGGCGACGTTGCCAGAGTACAGCTCGGACCGGAAGCCCGACggg                                                                >  2:356293/1‑128 (MQ=255)
                                                                                                       gCGGGCCTCGGGCTACCTGAAGACACTGGACGACTTTCGCGCCATCCCCCTGCGCCTGGCTGCCAACGGTGCACCGGTGATGCTGGGCGACGTTGCCAGAGTACAGCTCGGACCGGAAGCCCGACggg                                                                <  1:356293/128‑1 (MQ=255)
                                                                                                         gggCCTCGGGCTACCTGAAGACACTGGACGACTTTCGCGCCATCCCCCTGCGCCTGGCTGCCAACGGTGCACCGGTGATGCTGGGCGACGTTGCCAGAGTACAGCTCGGACCGGAAGCCCGACGGGGTATCGGAGAGCTGGACggcgaa                                         <  3:119872/149‑1 (MQ=255)
                                                                                                           gCCTCGGGCTACCTGAAGACACTGGACGACTTTCGCGCCATCCCCCTGCGCCTGGCTGCCAACGGTGCACCGGTGATGCTGGGCGACGTTGCCAGAGTACAGCTCGGACCGGAAGCCCGACGGGGTATCGGAGAGCTGGACggcgaagg                                       <  3:318324/149‑1 (MQ=255)
                                                                                                             cTCGGGCTACCTGAAGACACTGGACGACTTTCGCGCCATCCCCCTGCGCCTGGCTGCCAACGGTGCACCGGTGATGCTGGGCGACGTTGCCAGAGTACAGCTCGGACCGGAAGCCCGACGGGGTATCGGAGAGCTGGACggcgaag                                        >  4:282228/1‑146 (MQ=255)
                                                                                                             cTCGGGCTACCTGAAGACACTGGACGACTTTCGCGCCACCCCCCTGCGCCTGGCTGCCAACGGTGCACCGGTGATGCTGGGCGACGTTGCCAGAGTACAGCTCGGACCgg                                                                            <  5:192840/110‑1 (MQ=255)
                                                                                                             cTCGGGCTACCTGAAGACACTGGACGACTTTCGCGCCACCCCCCTGCGCCTGGCTGCCAACGGTGCACCGGTGATGCTGGGCGACGTTGCCAGAGTACAGCTCGGACCgg                                                                            >  6:192840/1‑110 (MQ=255)
                                                                                                                               acTGGACGACTTTCGCGCCATCCCCCTGCGCCTGGCTGCCAACGGTGCACCGGTGATGCTGGGCGACGTTGCCAGAGTACAGCTCGGACCGGAAGCCCGACGGGGTATCGGAGAGCTGGACggcgaaggcgaa                                   <  4:3541/133‑1 (MQ=255)
                                                                                                                               acTGGACGACTTTCGCGCCATCCCCCTGCGCCTGGCTGCCAACGGTGCACCGGTGATGCTGGGCGACGTTGCCAGAGTACAGCTCGGACCGGAAGCCCGACGGGGTATCGGAGAGCTGGACggcgaaggcgaa                                   >  3:3541/1‑133 (MQ=255)
                                                                                                                               acTGGACGACTTTCGCGCCACCCCCCTGCGCCTGGCTGCCAACGGTGCACCGGTGATGCTGGGCGACGTTGCCa                                                                                              <  5:340381/74‑1 (MQ=255)
                                                                                                                               acTGGACGACTTTCGCGCCACCCCCCTGCGCCTGGCTGCCAACGGTGCACCGGTGATGCTGGGCGACGTTGCCa                                                                                              >  6:340381/1‑74 (MQ=255)
                                                                                                                                        cTTTCGCGCCATCCCCCTGCGCCTGGCTGCCAACGGTGCACCGGTGATGCTGGGCGACGTTGCCAGAGTACAGCTCGGACCGGAAGCCCGACGGGGTATCGGAGAGCTGGACGGCGAAGGCGAAGCCGTGGgtggtg                      <  4:299745/137‑1 (MQ=255)
                                                                                                                                        cTTTCGCGCCATCCCCCTGCGCCTGGCTGCCAACGGTGCACCGGTGATGCTGGGCGACGTTGCCAGAGTACAGCTCGGACCGGAAGCCCGACGGGGTATCGGAGAGCTGGACGGCGAAGGCGAAGCCGTGGgtggtg                      >  3:299745/1‑137 (MQ=255)
                                                                                                                                        cTTTCGCGCCATCCCCCTGCGCCTGGCTGCCAACGGTGCACCGGTGATGCTGGGCGACGTTGCCAGAGTACAGCTCGGACCGGAAGCCCGACGGGGTATCGGAGAGCTGGACGGCGAAGGCGAAGCCGTGGGTGGTGTGGTGATCCTgc          >  4:357110/1‑149 (MQ=255)
                                                                                                                                        cTTTCGCGCCATCCCCCTGCGCCTGGCTGCCAACGGTGCACCGGTGATGCTGGGCGACGTTGCCAGAGTACAGCTCGGACCGGAAGCCCGACGGGGTATCGGAGAGCTGGACGGCGAAGGCGAAGCCGTGGGTGGTGTGGTGATCCTgc          >  2:35483/1‑149 (MQ=255)
                                                                                                                                             gcgcCATCCCGATGCGCCTGTCTGCCAACGGTGCACCGGTGATGCTGGGCGACTTTGCCAGAGTAGAGCTCGGACCGGAAGCCAGACGGGGTATCGGAGAGCTGGACGGCGAAGGCGAAGCCGTGGGTGGTGTGGTGATCCTGCGCAGt     <  5:299979/149‑1 (MQ=255)
                                                                                                                                              cgcCATCCCCCTGCGCCTGGCTGCCAACGGTGCACCGGTGATGCTGGGCGACGTTGCCAGAGTACAGCTCGGACCGGAAGCCCGACGGGGTATCGGAGAGCTGGACGGCGAAGGCGAAGCCGTGGGTGGTGTGGTGATCCTGCGCAGTg    <  6:216865/149‑1 (MQ=255)
                                                                                                                                                  aTCCCCCTGCGCCTGGCTGCCAACGGTGCACCGGTGATGCTGGGCGACGTTGCCAGAGTACAGCTCGGACCGGAAGCCCGACGGGGTATCGGAGAGCTGGACGGCGAAGGCGAAGCCGTGGGTGGTGTGGTGATCCTGCGCAGTGGc  >  3:135436/1‑147 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
CCAGCCTCGGCATCACCCAGGGCGAGGTCGCAAACGCCATCGGTAAGGCCAATCAGGAAACAGGCGGTGGCGTGCTGGAACAGGGCGAGGCGGAGTTCATGGTGCGGGCCTCGGGCTACCTGAAGACACTGGACGACTTTCGCGCCATCCCCCTGCGCCTGGCTGCCAACGGTGCACCGGTGATGCTGGGCGACGTTGCCAGAGTACAGCTCGGACCGGAAGCCCGACGGGGTATCGGAGAGCTGGACGGCGAAGGCGAAGCCGTGGGTGGTGTGGTGATCCTGCGCAGTGGC  >  NC_002947/6140854‑6141146

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑