Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 6,166,180 T→A 5.4% Q21L (CAG→CTG)  PP_5406 ← transposase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029476,166,1800TA 5.4% 233.8 / 2.1 74Q21L (CAG→CTG) PP_5406transposase
Reads supporting (aligned to +/- strand):  ref base T (40/30);  new base A (2/2);  total (42/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.40e-01

TGGTAGAGAGCTAATTGCGCAAACACCTTCTCTGGGTTGGAAAGGAGATGAATTGTGCGCCCCAGCTTTCGACTATGCAGTCTGCATATGCGAGAGCCTTTAGGTGCCTCTCGTGGAACCGCCAGCGTGGACGGTACATATGCCTGGCCCCATTCGAACCGGGCCTGGCGTTTCATGATCAGGTCA‑AATCGTTCCTGCGGTTTCATCCAAGCTTTCCAATCTCACTAGGATTTCCTGCCGAAGGCACGTTGGGGATAACAACACGCACACCGGCAAAACCCTTATCCTGCG  >  NC_002947/6166036‑6166326
                                                                                                                                                |                                                                                                                                                   
tggtAGAGAGCTAATTGCGCAAACACCTTCTCTGGGTTGGAAAGGAGATGAATTGTGCGCCCCAGCTTTCGACTATGCAGTCTGCATATGCGAGAGCCTTTAGGTGCCTCTCGTGGAACCGCCAGCGTGGACGGTACATATGCCTGGcc                                                                                                                                                 <  7:264248/149‑1 (MQ=255)
      agagCTAATTGCGCAAACACCTTCTCTGGGTTGGAAAGGAGATGAATTGTGCGCCCCAGCTTTCGACTATGCAGTCTGCATATGCGAGAGCCTTTAGGTGCCTCTCGTGGAACCGCCAGCGTGGACGGTACATATGCCTGGCCCCATTc                                                                                                                                           <  4:347883/149‑1 (MQ=255)
       gagCTAATTGCGCAAACACCTTCTCTGGGTTGGAAAGGAGATGAATTGTGCGCCCCAGCTTTCGACTATGCAGTCTGCATATGCGAGAGCCTTTAGGTGCCTCTCGTGGAACCGCCAGCGTGGACGGTACATATGCCTGGCCCCATTc                                                                                                                                           >  8:182828/1‑148 (MQ=255)
                    aaaCACCTTCTCTGGGTTGGAAAGGAGATGAATTGTGCGCCCCAGCTTTCGACTATGCAGTCTGCATATGCGAGAGCCTTTAGGTGCCTCTCGTGGAACCGCCAGCGTGGACGGTACATATGCCTGGCCCCATTCGAACCgg                                                                                                                                    <  5:217614/142‑1 (MQ=255)
                    aaaCACCTTCTCTGGGTTGGAAAGGAGAAGAATTGTGCGCCCCAGCTTTCGACTATGCAGTCTGCATATGCGAGAGCCTTTAGGTGCCTCTCGTGGAACCGCCACCGTGGACGGTACATATGCCTGGCCCCATTCGAACCgg                                                                                                                                    >  6:217614/1‑142 (MQ=255)
                          cTTCTCTGGGTTGGAAAGGAGATGAATTGTGCGCCCCAGCTTTCGACTATGCAGTCTGCATATGCGAGAGCCTTTAGGTGCCTCTCGTGGAACCGCCAGCGTGGACGGTACATATGCCTGGCCCCATTCGAACCGGGCCTGGCGTTTCa                                                                                                                       >  8:331875/1‑149 (MQ=255)
                                      ggAAAGGAGATGAATTGTGCGCCCCAGCTTTCGACTATGCAGTCTGCATATGCGAGAGCCTTTAGGTGCCTCTCGTGGAACCGCCAGCGTGGACGGTACATATGCCTGGCCCCATTCGAACCGGGCCTGGCGTTTCATGATCAGGTCa                                                                                                            >  5:189597/1‑148 (MQ=255)
                                       gAAAGGAGATGAATTGTGCGCCCCAGCTTTCGACTATGCAGTCTGCATATGCGAGAGCCTTTAGGTGCCTCTCGTGGAACCGCCAGCGTGGACGGTACATATGCCTGGCCCCATTCGAACCGGGCCTGGCGTTTCATGATCAGGTCa‑aa                                                                                                         >  4:161110/1‑149 (MQ=255)
                                         aaGGAGATGAATTGTGCGCCCCAGCTTTCGACTATGCAGTCTGCATATGCGAGAGCCTTTAGGTGCCTCTCGTGGAACCGCCAGCGTGGACGGTACATATGCCTGGCCCCATTCGAACCGGGCCTGGCGTTTCATGATCAGGTCA‑AATc                                                                                                       <  7:182828/149‑1 (MQ=255)
                                         aaGGAGATGAATTGTGCGCCCCAGCTTTCGACTATGCAGTCTGCATATGCGAGAGCCTTTAGGTGCCTCTCGTGGAACCGCCAGCGTGGACGGTACATATGCCTGGCCCCATTCGAACCGGGCCTGGCGTTTCATGATCAGGTCA‑AATc                                                                                                       <  5:65256/149‑1 (MQ=255)
                                           ggAGATGAATTGTGCGCCCCAGCTTTCGACTATGCAGTCTGCATATGCGAGAGCCTTTAGGTGCCTCTCGTGGAACCGCCAGCGTGGACGGTACATATGCCTGGCCCCATTCGAACCGGGCCTGGCGTTTCATGATCAGGTCA‑AATCGt                                                                                                     <  6:189597/149‑1 (MQ=255)
                                              gaTGAATTGTGCGCCCCAGCTTTCGACTATGCAGTCTGCATATGCGAGAGCCTTTAGGTGCCTCTCGTGGAACCGCCAGCGTGGACGGTACATATGCCTGGCCCCATTCGAACCGGGCCTGGCGTTTCa                                                                                                                       <  8:253773/129‑1 (MQ=255)
                                              gaTGAATTGTGCGCCCCAGCTTTCGACTATGCAGTCTGCATATGCGAGAGCCTTTAGGTGCCTCTCGTGGAACCGCCAGCGTGGACGGTACATATGCCTGGCCCCATTCGAACCGGGCCTGGCGTTTCa                                                                                                                       >  7:253773/1‑129 (MQ=255)
                                                  aaTTGTGCGCCCCAGCTTTCGACTATGCAGTCTGCATATGCGAGAGCCTTTAGGTGCCTCTCGTGGAACCGCCAGCGTGGACGGTACATATGCCTGGCCCCATTCGAACCGGGCCTGGCGTTTCATGATCAGGTCA‑AATCGTTCCTGCg                                                                                              >  4:259602/1‑149 (MQ=255)
                                                        gcgcCCCAGCTTTCGACTATGCAGTCTGCATATGCGAGAGCCTTTAGGTGCCTCTCGTGGAACCGCCAGCGTGGACGGTACATATGCCTGGCCCCATTCGAACCGGGCCTGGCGTTTCATGATCAGGTCA‑AATCGTTCCTGCGGTTTCa                                                                                        >  3:152424/1‑149 (MQ=255)
                                                          gcCCCAGCTTTCGACTATGCAGTCTGCATATGCGAGAGCCTTTAGGTGCCTCTCGTGGAACCGCCAGCGTGGACGGTACATATGCCTGGCCCCATTCGAACCGGGCCTGGCGTTTCATGATCAGGTCA‑AATCGTTCCTGCGGTTTCATc                                                                                      <  3:259602/149‑1 (MQ=255)
                                                             ccAGCTTTCGACTATGCAGTCTGCATATGCGAGAGCCTTTAGGTGCCTCTCGTGGAACCGCCAGCGTGGACGGTACATATGCCTGGCCCCATTCGAACCGGGCCTGGCGTTTCATGATCAGGTCA‑AATCGTTCCTGCGGTTTCATCCaa                                                                                   <  3:161110/149‑1 (MQ=255)
                                                              cAGCTTTCGACTATGCAGTCTGCATATGCGAGAGCCTTTAGGTGCCTCTCGTGGAACCGCCAGCGTGGACGGTACATATGCCAGGCCCGGTTCGAATGGGGCCAgg                                                                                                                              <  1:100539/106‑1 (MQ=38)
                                                              cAGCTTTCGACTATGCAGTCTGCATATGCGAGAGCCTTTAGGTGCCTCTCGTGGAACCGCCAGCGTGGACGGTACATATGCCAGGCCCGGTTCGAATGGGGCCAgg                                                                                                                              <  1:105028/106‑1 (MQ=38)
                                                              cAGCTTTCGACTATGCAGTCTGCATATGCGAGAGCCTTTAGGTGCCTCTCGTGGAACCGCCAGCGTGGACGGTACATATGCCAGGCCCGGTTCGAATGGGGCCAgg                                                                                                                              >  2:105028/1‑106 (MQ=38)
                                                              cAGCTTTCGACTATGCAGTCTGCATATGCGAGAGCCTTTAGGTGCCTCTCGTGGAACCGCCAGCGTGGACGGTACATATGCCAGGCCCGGTTCGAATGGGGCCAgg                                                                                                                              >  2:100539/1‑106 (MQ=38)
                                                               aGCTTTCGACTATGCAGTCTGCATATGCGAGAGCCTTTAGGTGCCTCTCGTGGAACCGCCAGCGTGGACGGTACATATGCCTGGCCCCATTCGAACCGGGCCTGGCGTTTCATGATCAGGTCA‑AATCGTTCCTGCGGTTTCATCCAAGc                                                                                 >  2:292222/1‑149 (MQ=255)
                                                                    tctaCTATGCAGTCTGCATATGCGAGAGCCTTTAGGTGCCTCTCGTGGAACCGACAGCGTGGACGGTACATATTCCTGTCCCCATTCGAACCGGGCCTGGCGTTTCATGATCAGGTCA‑AATCGTTCCTGCGGTTTCATCCAAGCTTTc                                                                             <  5:283926/145‑1 (MQ=255)
                                                                    tCGACTATGCAGTCTGCATATGCGAGAGCCTTTAGGTGCCTCTCGTGGAACCGCCAGCGTGGACGGTACATATGCCTGGCCCCATTCGAACCGGGCCTGGCGTTTCATGATCAGGTCA‑AATCGTTc                                                                                                   <  8:105391/126‑1 (MQ=255)
                                                                    tCGACTATGCAGTCTGCATATGCGAGAGCCTTTAGGTGCCTCTCGTGGAACCGCCAGCGTGGACGGTACATATGCCTGGCCCCATTCGAACCGGGCCTGGCGTTTCATGATCAGGTCA‑AATCGTTc                                                                                                   >  7:105391/1‑126 (MQ=255)
                                                                      gACTATGCAGTCTGCATATGCGAGAGCCTTTAGGTGCCTCTCGTGGAACCGCCAGCGTGGACGGTACATATGCCTGGCCCCATTCGAACCGGGCCTGGCGTTTCATGATCAGGTCA‑AATCGTTCCTGCGGTTTCATCCAAGCTTTCCaa                                                                          >  5:148522/1‑149 (MQ=255)
                                                                       aCTATGCAGTCTGCATATGCGAGAGCCTTTAGGTGCCTCTCGTGGAACCGCCAGCGTGGACGGTACATATGCCTGGCCCCATTCGAACCGGGCCTGGCGTTTCATGATCAGGTCA‑AATCGTTCCTGCGGTTTCATCCAAGCTTTCCAAt                                                                         >  7:47187/1‑149 (MQ=255)
                                                                       aCTATGCAGTCTGCATATGCGAGAGCCTTTAGGTGCCTCTCGTGGAACCGCCAGCGTGGACGGTACATATGCCTGGCCCCATTCGAACCGGGCCTGGCGTTTCATGATCAGGTCA‑AATCGTTCCTGCGGTTTCATCCAAGCTTTCCAAt                                                                         >  7:52220/1‑149 (MQ=255)
                                                                              aGTCTGCATATGCGAGAGCCTTTAGGTGCCTCTCGTGGAACCGCCAGCGTGGACGGTACATATGCCTGGCCCCATTCGAACCGGGCCTGGCGTTTCATGATCAGGTCA‑AATCGTTCCTGCGGTTTCATCCAAGCTTTCCAATCTCAc                                                                    >  5:50991/1‑147 (MQ=255)
                                                                              aGTCTGCATATGCGAGAGCCTTTAGGTGCCTCTCGTGGAACCGCCAGCGTGGACGGTACATATGCCTGGCCCCATTCGAACCGGGCCTGGCGTTTCATGATCAGGTCA‑AATCGTTCCTGCGGTTTCATCCAAGCTTTCCAATCTCACTa                                                                  <  4:152424/149‑1 (MQ=255)
                                                                                     atatGCGAGAGCCTTTAGGTGCCTCTCGTGGAACCGCCAGCGTGGACGGTACATATGCCTGGCCCCATTCGAACCGGGCCTGGCGTTTCATGATCa                                                                                                                 <  3:119888/96‑1 (MQ=255)
                                                                                     atatGCGAGAGCCTTTAGGTGCCTCTCGTGGAACCGCCAGCGTGGACGGTACATATGCCTGGCCCCATTCGAACCGGGCCTGGCGTTTCATGATCa                                                                                                                 >  4:119888/1‑96 (MQ=255)
                                                                                     atatGCGAGAGCCTTTAGGTGCCTCTCGTGGAACCGCCAGCGTGGACGGTACATATGCCTGGCCCCATTCGAACCGGGCCTGGCGTTTCATGATCAGGTCA‑AATCGTTCCTGCGGTTTCATCCAAGCTTTCCAATCTCACTAGGATTTc                                                           >  6:161365/1‑149 (MQ=255)
                                                                                        tGCGAGAGCCTTTAGGTGCCTCTCGTGGAACCGCCAGCGTGGACGGTACATATGCCTGGCCCCATTCGAACCGGGCCTGGCGTTTCATGATCAGGTCA‑AATCGTTCCTGCGGTTTCATCCAAGCTTTCCAATCTCACTAGGATTTCCTg                                                        >  5:215274/1‑149 (MQ=255)
                                                                                          cGAGAGCCTTTAGGTGCCTCTCGTGGAACCGCCAGCGTGGACGGTACATATGCCTGGCCCCATTCGAACCGGGCCTGGCGTTTCATGATCAGGTCA‑AATCGTTCCTGCGGTTTCATCCAAGCTTTCCAATCTCACTAGGATTTCCTGc                                                       <  8:249179/148‑1 (MQ=255)
                                                                                            agagCCTTTAGGTGCCTCTCGTGGAACCGCCAGCGTGGACGGTACATATGCCTGGCCCCATTCGAACCGGGCCTGGCGTTTCATGATCAGGTCA‑AATCGTTCCTGCGGTTTCATCCAAGCTTTCCAAtct                                                                       <  5:71973/130‑1 (MQ=255)
                                                                                            agagCCTTTAGGTGCCTCTCGTGGAACCGCCAGCGTGGACGGTACATATGCCTGGCCCCATTCGAACCGGGCCTGGCGTTTCATGATCAGGTCA‑AATCGTTCCTGCGGTTTCATCCAAGCTTTCCAAtct                                                                       >  6:71973/1‑130 (MQ=255)
                                                                                                ccTTTAGGTGCCTCTCGTGGAACCGCCAGCGTGGACGGTACATATGCCTGGCCCCATTCGAACCGGGCCTGGCGTTTCATGATCAGGTCA‑AATCGTTCCTGCGGTTTCATCCAAGCTTTCCAATCTCACTAGGATTTCCTGCCGAAGGc                                                <  6:148522/149‑1 (MQ=255)
                                                                                                  tttAGGTGCCTCTCGTGGAACCGCCAGCGTGGACGGAACATATGCCTGGCCCCATTCGAACCGGGCCTGGCGTTTCATGATCAGGTCA‑AATCGTTCCTGCGGTTTCATCCAAGCTTTCCAATCTCACTAGGATTTCCTGCCGAAGGCAc                                              >  5:102631/1‑149 (MQ=255)
                                                                                                      ggTGCCTCTCGTGGAACCGCCAGCGTGGACGGTACATATGCCTGGCCCCATTCGAACCGGGCCTGGCGTTTCATGATCAGGTCA‑AATCGTTCCTGCGGTTTCATCCAAGCTTTCCAATCTCACTAGGATTTCCTGCCGAAGGCACGTTg                                          >  1:235814/1‑149 (MQ=255)
                                                                                                       gTGCCTCTCGTGGAACCGCCAGCGTGGACGGTACATATGCCTGGCCCCATTCGAACCGGGCCTGGCg                                                                                                                            >  6:280806/1‑67 (MQ=255)
                                                                                                       gTGCCTCTCGTGGAACCGCCAGCGTGGACGGTACATATGCCTGGCCCCATTCGAACCGGGCCTGGCg                                                                                                                            <  5:280806/67‑1 (MQ=255)
                                                                                                        tGCCTCTCGTGGAACCGCCAGCGTGGACGGTACATATGCCTGGCCCCATTCGAACCGGGCCTGGCGTTTCATGATCAGGTCA‑AATCGTTCCTGCGGTTTCATCCAAGCTTTCCAATCTCACTAGGATTTCCTGCCGAAGGCACGTTggg                                        <  6:215274/149‑1 (MQ=255)
                                                                                                          ccTCTCGTGGAACCGCCAGCGTGGACGGTACATATGCCTGGCCCCATTCGAACCGGGCCTGGCGTTTCATGATCAGGTCA‑AATCGTTCCTGCGGTTTCATCCAAGCTTTCCAATCTCACTAGGATTTCCTGCCGAAGGCACGTTGGGGa                                      <  1:292222/149‑1 (MQ=255)
                                                                                                           ctctCGTGGAACCGCCAGCGTGGACGGTACATATGCCTGGCCCCATTCGAACCGGGCCTGGCGTTTCATGATCAGGTCA‑AATCGTTCCTGCGGTTTCATCCAAGCTTTCCAATCTCACTAGGATTTCCTGCCGaa                                                   <  6:205568/135‑1 (MQ=255)
                                                                                                           ctctCGTGGAACCGCCAGCGTGGACGGTACATATGCCTGGCCCCATTCGAACCGGGCCTGGCGTTTCATGATCAGGTCA‑AATCGTTCCTGCGGTTTCATCCAAGCTTTCCAATCTCACTAGGATTTCCTGCAGaa                                                   >  5:205568/1‑135 (MQ=255)
                                                                                                            tctcGTGGAACCGCCAGCGTGGATGGTACATATGCCTGGCCCCATTCGAACCGGGCCTGGCGTTTCATGATCAGGTCA‑AATCGTTCCTGCGGTTTCATCCAAGCTTTCCAATCTCACTAGGATTTCCTGCCGAAGGCACGTTgggg                                       >  8:27916/1‑146 (MQ=255)
                                                                                                            tctcGTGGAACCGCCAGCGTGGATGGTACATATGCCTGGCCCCATTCGAACCGGGCCTGGCGTTTCATGATCAGGTCA‑AATCGTTCCTGCGGTTTCATCCAAGCTTTCCAATCTCACTAGGATTTCCTGCCGAAGGCACGTTgggg                                       <  7:27916/146‑1 (MQ=255)
                                                                                                            tctcGTGGAACCGCCAGCGTGGACGGTACATATGCCTGGCCCCATTCGAACCGGGCCTGGCGTTTCATGATCAGGTCA‑AATCGTTCCTGCGGTTTCATCCAAGCTTTCCAATCTCACTAGGATTTCCTGCCGAAGGCACGTTGGGGAt                                     >  3:77311/1‑148 (MQ=255)
                                                                                                             ctcGTGGAACCGCCAGCGTGGACGGTACATATGCCTGGCCCCATTCGAACCGGGCCTGGCGTTTCATGATCAGGTCA‑AATCGTTCCTGCGGTTTCATCCAAGCTTTCCAATCTCACTAGGATTTCCTGCCGAAGGCACGTTGGGGATaa                                   >  5:282366/1‑149 (MQ=255)
                                                                                                                 tGGAACCGCCAGCGTGGACGGTACATATGCCTGGCCCCATTCGAACCGGGCCTGGCGTTTCATGATCAGGTCA‑AATCGTTCCTGCGGTTTCATCCAAGCTTTCCAATCTCACTAGGATTTCCTGCCGAAGGCACGTTGGGGATaacaa                                >  1:341977/1‑148 (MQ=255)
                                                                                                                   gAACCGCCAGCGTGGACGGTACATATGCCTGGCGCCATTCGAACCGGGACTGGCGTTTAATGATCAGGTCA‑AATCGTTCCTGCGGTTTCATCCAAGATTTCCAATCTCACTACGATTTCCTGCCGAAGGCACGTTGGGGATAAcaccac                             >  6:86092/1‑149 (MQ=255)
                                                                                                                         ccAGCGTGGACGGTACATATGCCTGGCCCCATTCGAACTGGGCCTGGCGTTTCATGATCAGGTCA‑AATCGTTCCTGCGGTTTCATCCAAGCTTTCCAATCTCACTAGGATTTCCTGCCGAAGGCACGTTGGGGATAACAACACGCacac                       >  8:153712/1‑149 (MQ=255)
                                                                                                                         ccAGCGTGGACGGTACATATGCCTGGCCCCATTCGAACCGGGCCTGGCGTTTCATGATCAGGTCA‑AATCGTTCCTGCGGTTTCATCCAAGCTTTCCAATCTCACTAGGATTTCCt                                                         >  1:239207/1‑115 (MQ=255)
                                                                                                                         ccAGCGTGGACGGTACATATGCCTGGCCCCATTCGAACCGGGCCTGGCGTTTCATGATCAGGTCA‑AATCGTTCCTGCGGTTTCATCCAAGCTTTCCAATCTCACTAGGATTTCCt                                                         <  2:239207/115‑1 (MQ=255)
                                                                                                                         ccAGCGTGGACGGTACATATGCCTGGCCCCATTCGAACCGGGCCTGGCGTTTCATGATCAGGTCA‑AATCGTTCCTGCGGTTTCATCCAAGCTTTCCAATCTCACTAGGATTTCCTGCCGAAGGCACGTTGGGGAt                                     >  7:16758/1‑135 (MQ=255)
                                                                                                                         ccAGCGTGGACGGTACATATGCCTGGCCCCATTCGAACCGGGCCTGGCGTTTCATGATCAGGTCA‑AATCGTTCCTGCGGTTTCATCCAAGCTTTCCAATCTCACTAGGATTTCCTGCCGAAGGCACGTTGGGGAt                                     <  8:16758/135‑1 (MQ=255)
                                                                                                                         ccAGCGTGGACGGTACATATGCCTGGCCCCATTCGAACCGGGCCTGGCGTTTCATGATCAGGTCA‑AATCGTTCCTGCGGTTTCATCCAAGCTTTCCAATCTCACTAGGATTTCCTGCCGAAGGCACGTTGGGGATACCAACACGCacac                       >  2:268344/1‑149 (MQ=255)
                                                                                                                         ccAGCGTGGACGGTACATATGCCTGGCCCCATTCGAACCGGGCCTGGCGTTTCATGATCAGGTCA‑AATCGTTCCTGCGGTTTCATCCAAGCTTTCCAATCTCACTAGGATTTCCTGCCGAAGGCACGTTGGGGATAACAACACGCacac                       >  2:272991/1‑149 (MQ=255)
                                                                                                                          cAGCGTGGACGGTACATATGCCTGGCCCCATTCGAACCGGGCCTGGCGTTTCATGATCAGGTCA‑AATCGTTCCTGCGGTTTCATCCAAGCTTTCCAATCTCACTAGGATTTCCTGCCGAAGGCACGTTGGGGATAACAACACGCACAcc                      >  4:59791/1‑149 (MQ=255)
                                                                                                                                   cGGTACATATGCCTGGCCCCATTCGAACCGGGCCTGGCGTTTCATGATCAGGTCA‑AATCGTTCCTGCGGTTTCATCCAAGCTTTCCAATCTCACTAGGATTTCCTGCCGAAGGCACGTTGGGGATAACAACACGCACACCGGCAAAAcc             <  4:77311/149‑1 (MQ=255)
                                                                                                                                   cGGTACATATGCCTGGCCCCATTCGAACCGGGCCTGGCGTTTCATGATCAGGTCA‑AATCGTTCCTGCGGTTTCATCCAAGCTTTCCAATCTCAATAGGATTTCCTGCCGAAGGCACGTTGGGGATAACAACACGCACACCGGCAAAAcc             <  2:341977/149‑1 (MQ=255)
                                                                                                                                     gTACATATGCCTGGCCCCATTCGAACCGGGCCTGGCGTTTCATGATCAGGTCA‑AATCGTTCCTGCGGTTTCATCCAAGCTTTCCAATCTCACTAGGATTTCCTGCCGAAGGCACGTTGGGGATAACAACACGCACACCGGCAAAACCCt           >  8:192249/1‑149 (MQ=255)
                                                                                                                                      tACATATGCCTGGCCCCATTCGAACTGGGCCTGGCGTTTCATGATCAGGTCA‑AATCGTTCCTGCGGTTTCATCCAAGCTTTCCAATCTCACTAGGATTTCCTGCCGAAGGCACGTTGGGGATAACAACACGCACACCGGCAAAACCCtt          <  7:153712/149‑1 (MQ=255)
                                                                                                                                      tACATATGCCTGGCCCCATTCGAACCGGGCCTGGCGTTTCATGATCAGGTCA‑AATCGTTCCTGCGGTTTCATCCAAGCTTTCCAATCTCACTAGGATTTCCTGCCGAAGGCACGTTGGGGATAACAACACGCACACCGGCAAAACCCt           >  3:121809/1‑148 (MQ=255)
                                                                                                                                         atatGCCTGGCCCCATTCGAACCGGGCCTGGCGTTTCATGATCAGGTCA‑AATCGTTCCTGCGGTTTCATCCAAGCTTTCCAATCTCACTAGGATTTCCTGCCGAAGGCACGTTGGGGATAACAACACGCACACCGGCAAAACCCtt          >  7:175642/1‑146 (MQ=255)
                                                                                                                                         atatGCCTGGCCCCATTCGAACCGGGCCTGGCGTTTCATGATCAGGTCA‑AATCGTTCCTGCGGTTTCATCCAAGCTTTCCAATCTCACTAGGATTTCCTGCCGAAGGCACGTTGGGGATAACAACACGCACACCGGCAAAACCCtt          <  8:175642/146‑1 (MQ=255)
                                                                                                                                         atatGCCTGGCCCCATTCGAACCGGGCCTGGCGTTTCATGATCAGGTCA‑AATCGTTCCTGCGGTTTCATCCAAGCTTTCCAATCTCACTAGGATTTCCTGCCGAAGGCACGTTGGGGATAACAACACGCACACCGGCAAAACCCTTATc       >  3:293951/1‑149 (MQ=255)
                                                                                                                                           atGCCTGGCCCCATTCGAACCGGGCCTGGCGTTTCATGATCAGGTCACAATCGTTCCTGCGGTTTCATCCAAGCTTTCCAATCTCACTAGGATTTCCTGCCGAAGGCACGTTGGGGATAACAACACGCACACCGGCAAAACCCTTATcc      >  1:79225/1‑149 (MQ=255)
                                                                                                                                           atGCCTGGCCCCATTCGAACCGGGCCTGGCGTTTCATGATCAGGTCA‑AATCGTTCCTGCGGTTTCATCCAAGCTTTCCAATCTCACTAGGATTTCCTGCCGAAGGCACGTTGGGGATAACAACACGCACACCGGCAAAACCCTTATCCt     >  3:235403/1‑149 (MQ=255)
                                                                                                                                              ccTGGCCCCATTCGAACCGGGCCTGGCGTTTCATGATCAGGTCA‑AATCGTTCCTGCGGTTTCATCCAAGCTTTCCAATCTCACCAGGATTTCCTGCCGAAGGCACGTTGGGGATAACAACACGCACACCGGCAAAACCCTTATCCTGCg  <  8:47187/149‑1 (MQ=255)
                                                                                                                                              ccTGGCCCCATTCGAACCGGGCCTGGCGTTTCATGATCAGGTCA‑AATCGTTCCTGCGGTTTCATCCAAGCTTTCCAATCTCACCAGGATTTCCTGCCGAAGGCACGTTGGGGATAACAACACGCACACCGGCAAAACCCTTATCCTGCg  <  8:52220/149‑1 (MQ=255)
                                                                                                                                               cTGGCCCCATTCGAACCGGGCCTGGCGTTTCATGATCAGGTCA‑AATCGTTCCTGCGGTTTCATCCAAGCTTTCCAATCTCACTAGGATTTCCTGCCGAAGGCACGTTGGGGATAACAACACGCACACCGGCAAAACCCTTa         >  3:191693/1‑141 (MQ=255)
                                                                                                                                               cTGGCCCCAATCGAACCGGGCCTGGCGTTTCATGATCAGGTCA‑AATCGTTCCTGCGGTTTCATCCAAGCTTTCCAATCTCACTAGGATTTCCTGCCGAAGGCACGTTGGGGATAACAACACGCACACCGGCAAAACCCTTa         <  4:191693/141‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                   
TGGTAGAGAGCTAATTGCGCAAACACCTTCTCTGGGTTGGAAAGGAGATGAATTGTGCGCCCCAGCTTTCGACTATGCAGTCTGCATATGCGAGAGCCTTTAGGTGCCTCTCGTGGAACCGCCAGCGTGGACGGTACATATGCCTGGCCCCATTCGAACCGGGCCTGGCGTTTCATGATCAGGTCA‑AATCGTTCCTGCGGTTTCATCCAAGCTTTCCAATCTCACTAGGATTTCCTGCCGAAGGCACGTTGGGGATAACAACACGCACACCGGCAAAACCCTTATCCTGCG  >  NC_002947/6166036‑6166326

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑