Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	6,166,186	C→G	5.4%	W19S (TGG→TCG) ‡	PP_5406 ←	transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	6,166,186	0	C	G	5.4%	239.0 / 3.6	74	W19S (TGG→TCG) ‡	PP_5406	transposase
Reads supporting (aligned to +/- strand):  ref base C (40/30);  new base G (2/2);  total (42/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.11e-01

AGAGCTAATTGCGCAAACACCTTCTCTGGGTTGGAAAGGAGATGAATTGTGCGCCCCAGCTTTCGACTATGCAGTCTGCATATGCGAGAGCCTTTAGGTGCCTCTCGTGGAACCGCCAGCGTGGACGGTACATATGCCTGGCCCCATTCGAACCGGGCCTGGCGTTTCATGATCAGGTCA‑AATCGTTCCTGCGGTTTCATCCAAGCTTTCCAATCTCACTAGGATTTCCTGCCGAAGGCACGTTGGGGATAACAACACGCACACCGGCAAAACCCTTATCCTGCGGGCAGG  >  NC_002947/6166042‑6166332                                                                                                                                                 |                                                                                                                                                    agagCTAATTGCGCAAACACCTTCTCTGGGTTGGAAAGGAGATGAATTGTGCGCCCCAGCTTTCGACTATGCAGTCTGCATATGCGAGAGCCTTTAGGTGCCTCTCGTGGAACCGCCAGCGTGGACGGTACATATGCCTGGCCCCATTc                                                                                                                                                 <  4:347883/149‑1 (MQ=255)  gagCTAATTGCGCAAACACCTTCTCTGGGTTGGAAAGGAGATGAATTGTGCGCCCCAGCTTTCGACTATGCAGTCTGCATATGCGAGAGCCTTTAGGTGCCTCTCGTGGAACCGCCAGCGTGGACGGTACATATGCCTGGCCCCATTc                                                                                                                                                 >  8:182828/1‑148 (MQ=255)               aaaCACCTTCTCTGGGTTGGAAAGGAGATGAATTGTGCGCCCCAGCTTTCGACTATGCAGTCTGCATATGCGAGAGCCTTTAGGTGCCTCTCGTGGAACCGCCAGCGTGGACGGTACATATGCCTGGCCCCATTCGAACCgg                                                                                                                                          <  5:217614/142‑1 (MQ=255)               aaaCACCTTCTCTGGGTTGGAAAGGAGAAGAATTGTGCGCCCCAGCTTTCGACTATGCAGTCTGCATATGCGAGAGCCTTTAGGTGCCTCTCGTGGAACCGCCACCGTGGACGGTACATATGCCTGGCCCCATTCGAACCgg                                                                                                                                          >  6:217614/1‑142 (MQ=255)                     cTTCTCTGGGTTGGAAAGGAGATGAATTGTGCGCCCCAGCTTTCGACTATGCAGTCTGCATATGCGAGAGCCTTTAGGTGCCTCTCGTGGAACCGCCAGCGTGGACGGTACATATGCCTGGCCCCATTCGAACCGGGCCTGGCGTTTCa                                                                                                                             >  8:331875/1‑149 (MQ=255)                                 ggAAAGGAGATGAATTGTGCGCCCCAGCTTTCGACTATGCAGTCTGCATATGCGAGAGCCTTTAGGTGCCTCTCGTGGAACCGCCAGCGTGGACGGTACATATGCCTGGCCCCATTCGAACCGGGCCTGGCGTTTCATGATCAGGTCa                                                                                                                  >  5:189597/1‑148 (MQ=255)                                  gAAAGGAGATGAATTGTGCGCCCCAGCTTTCGACTATGCAGTCTGCATATGCGAGAGCCTTTAGGTGCCTCTCGTGGAACCGCCAGCGTGGACGGTACATATGCCTGGCCCCATTCGAACCGGGCCTGGCGTTTCATGATCAGGTCa‑aa                                                                                                               >  4:161110/1‑149 (MQ=255)                                    aaGGAGATGAATTGTGCGCCCCAGCTTTCGACTATGCAGTCTGCATATGCGAGAGCCTTTAGGTGCCTCTCGTGGAACCGCCAGCGTGGACGGTACATATGCCTGGCCCCATTCGAACCGGGCCTGGCGTTTCATGATCAGGTCA‑AATc                                                                                                             <  5:65256/149‑1 (MQ=255)                                    aaGGAGATGAATTGTGCGCCCCAGCTTTCGACTATGCAGTCTGCATATGCGAGAGCCTTTAGGTGCCTCTCGTGGAACCGCCAGCGTGGACGGTACATATGCCTGGCCCCATTCGAACCGGGCCTGGCGTTTCATGATCAGGTCA‑AATc                                                                                                             <  7:182828/149‑1 (MQ=255)                                      ggAGATGAATTGTGCGCCCCAGCTTTCGACTATGCAGTCTGCATATGCGAGAGCCTTTAGGTGCCTCTCGTGGAACCGCCAGCGTGGACGGTACATATGCCTGGCCCCATTCGAACCGGGCCTGGCGTTTCATGATCAGGTCA‑AATCGt                                                                                                           <  6:189597/149‑1 (MQ=255)                                         gaTGAATTGTGCGCCCCAGCTTTCGACTATGCAGTCTGCATATGCGAGAGCCTTTAGGTGCCTCTCGTGGAACCGCCAGCGTGGACGGTACATATGCCTGGCCCCATTCGAACCGGGCCTGGCGTTTCa                                                                                                                             <  8:253773/129‑1 (MQ=255)                                         gaTGAATTGTGCGCCCCAGCTTTCGACTATGCAGTCTGCATATGCGAGAGCCTTTAGGTGCCTCTCGTGGAACCGCCAGCGTGGACGGTACATATGCCTGGCCCCATTCGAACCGGGCCTGGCGTTTCa                                                                                                                             >  7:253773/1‑129 (MQ=255)                                             aaTTGTGCGCCCCAGCTTTCGACTATGCAGTCTGCATATGCGAGAGCCTTTAGGTGCCTCTCGTGGAACCGCCAGCGTGGACGGTACATATGCCTGGCCCCATTCGAACCGGGCCTGGCGTTTCATGATCAGGTCA‑AATCGTTCCTGCg                                                                                                    >  4:259602/1‑149 (MQ=255)                                                   gcgcCCCAGCTTTCGACTATGCAGTCTGCATATGCGAGAGCCTTTAGGTGCCTCTCGTGGAACCGCCAGCGTGGACGGTACATATGCCTGGCCCCATTCGAACCGGGCCTGGCGTTTCATGATCAGGTCA‑AATCGTTCCTGCGGTTTCa                                                                                              >  3:152424/1‑149 (MQ=255)                                                     gcCCCAGCTTTCGACTATGCAGTCTGCATATGCGAGAGCCTTTAGGTGCCTCTCGTGGAACCGCCAGCGTGGACGGTACATATGCCTGGCCCCATTCGAACCGGGCCTGGCGTTTCATGATCAGGTCA‑AATCGTTCCTGCGGTTTCATc                                                                                            <  3:259602/149‑1 (MQ=255)                                                        ccAGCTTTCGACTATGCAGTCTGCATATGCGAGAGCCTTTAGGTGCCTCTCGTGGAACCGCCAGCGTGGACGGTACATATGCCTGGCCCCATTCGAACCGGGCCTGGCGTTTCATGATCAGGTCA‑AATCGTTCCTGCGGTTTCATCCaa                                                                                         <  3:161110/149‑1 (MQ=255)                                                         cAGCTTTCGACTATGCAGTCTGCATATGCGAGAGCCTTTAGGTGCCTCTCGTGGAACCGCCAGCGTGGACGGTACATATGCCAGGCCCGGTTCGAATGGGGCCAgg                                                                                                                                    <  1:100539/106‑1 (MQ=38)                                                         cAGCTTTCGACTATGCAGTCTGCATATGCGAGAGCCTTTAGGTGCCTCTCGTGGAACCGCCAGCGTGGACGGTACATATGCCAGGCCCGGTTCGAATGGGGCCAgg                                                                                                                                    >  2:105028/1‑106 (MQ=38)                                                         cAGCTTTCGACTATGCAGTCTGCATATGCGAGAGCCTTTAGGTGCCTCTCGTGGAACCGCCAGCGTGGACGGTACATATGCCAGGCCCGGTTCGAATGGGGCCAgg                                                                                                                                    >  2:100539/1‑106 (MQ=38)                                                         cAGCTTTCGACTATGCAGTCTGCATATGCGAGAGCCTTTAGGTGCCTCTCGTGGAACCGCCAGCGTGGACGGTACATATGCCAGGCCCGGTTCGAATGGGGCCAgg                                                                                                                                    <  1:105028/106‑1 (MQ=38)                                                          aGCTTTCGACTATGCAGTCTGCATATGCGAGAGCCTTTAGGTGCCTCTCGTGGAACCGCCAGCGTGGACGGTACATATGCCTGGCCCCATTCGAACCGGGCCTGGCGTTTCATGATCAGGTCA‑AATCGTTCCTGCGGTTTCATCCAAGc                                                                                       >  2:292222/1‑149 (MQ=255)                                                               tctaCTATGCAGTCTGCATATGCGAGAGCCTTTAGGTGCCTCTCGTGGAACCGACAGCGTGGACGGTACATATTCCTGTCCCCATTCGAACCGGGCCTGGCGTTTCATGATCAGGTCA‑AATCGTTCCTGCGGTTTCATCCAAGCTTTc                                                                                   <  5:283926/145‑1 (MQ=255)                                                               tCGACTATGCAGTCTGCATATGCGAGAGCCTTTAGGTGCCTCTCGTGGAACCGCCAGCGTGGACGGTACATATGCCTGGCCCCATTCGAACCGGGCCTGGCGTTTCATGATCAGGTCA‑AATCGTTc                                                                                                         <  8:105391/126‑1 (MQ=255)                                                               tCGACTATGCAGTCTGCATATGCGAGAGCCTTTAGGTGCCTCTCGTGGAACCGCCAGCGTGGACGGTACATATGCCTGGCCCCATTCGAACCGGGCCTGGCGTTTCATGATCAGGTCA‑AATCGTTc                                                                                                         >  7:105391/1‑126 (MQ=255)                                                                 gACTATGCAGTCTGCATATGCGAGAGCCTTTAGGTGCCTCTCGTGGAACCGCCAGCGTGGACGGTACATATGCCTGGCCCCATTCGAACCGGGCCTGGCGTTTCATGATCAGGTCA‑AATCGTTCCTGCGGTTTCATCCAAGCTTTCCaa                                                                                >  5:148522/1‑149 (MQ=255)                                                                  aCTATGCAGTCTGCATATGCGAGAGCCTTTAGGTGCCTCTCGTGGAACCGCCAGCGTGGACGGTACATATGCCTGGCCCCATTCGAACCGGGCCTGGCGTTTCATGATCAGGTCA‑AATCGTTCCTGCGGTTTCATCCAAGCTTTCCAAt                                                                               >  7:47187/1‑149 (MQ=255)                                                                  aCTATGCAGTCTGCATATGCGAGAGCCTTTAGGTGCCTCTCGTGGAACCGCCAGCGTGGACGGTACATATGCCTGGCCCCATTCGAACCGGGCCTGGCGTTTCATGATCAGGTCA‑AATCGTTCCTGCGGTTTCATCCAAGCTTTCCAAt                                                                               >  7:52220/1‑149 (MQ=255)                                                                         aGTCTGCATATGCGAGAGCCTTTAGGTGCCTCTCGTGGAACCGCCAGCGTGGACGGTACATATGCCTGGCCCCATTCGAACCGGGCCTGGCGTTTCATGATCAGGTCA‑AATCGTTCCTGCGGTTTCATCCAAGCTTTCCAATCTCAc                                                                          >  5:50991/1‑147 (MQ=255)                                                                         aGTCTGCATATGCGAGAGCCTTTAGGTGCCTCTCGTGGAACCGCCAGCGTGGACGGTACATATGCCTGGCCCCATTCGAACCGGGCCTGGCGTTTCATGATCAGGTCA‑AATCGTTCCTGCGGTTTCATCCAAGCTTTCCAATCTCACTa                                                                        <  4:152424/149‑1 (MQ=255)                                                                                atatGCGAGAGCCTTTAGGTGCCTCTCGTGGAACCGCCAGCGTGGACGGTACATATGCCTGGCCCCATTCGAACCGGGCCTGGCGTTTCATGATCa                                                                                                                       >  4:119888/1‑96 (MQ=255)                                                                                atatGCGAGAGCCTTTAGGTGCCTCTCGTGGAACCGCCAGCGTGGACGGTACATATGCCTGGCCCCATTCGAACCGGGCCTGGCGTTTCATGATCa                                                                                                                       <  3:119888/96‑1 (MQ=255)                                                                                atatGCGAGAGCCTTTAGGTGCCTCTCGTGGAACCGCCAGCGTGGACGGTACATATGCCTGGCCCCATTCGAACCGGGCCTGGCGTTTCATGATCAGGTCA‑AATCGTTCCTGCGGTTTCATCCAAGCTTTCCAATCTCACTAGGATTTc                                                                 >  6:161365/1‑149 (MQ=255)                                                                                   tGCGAGAGCCTTTAGGTGCCTCTCGTGGAACCGCCAGCGTGGACGGTACATATGCCTGGCCCCATTCGAACCGGGCCTGGCGTTTCATGATCAGGTCA‑AATCGTTCCTGCGGTTTCATCCAAGCTTTCCAATCTCACTAGGATTTCCTg                                                              >  5:215274/1‑149 (MQ=255)                                                                                     cGAGAGCCTTTAGGTGCCTCTCGTGGAACCGCCAGCGTGGACGGTACATATGCCTGGCCCCATTCGAACCGGGCCTGGCGTTTCATGATCAGGTCA‑AATCGTTCCTGCGGTTTCATCCAAGCTTTCCAATCTCACTAGGATTTCCTGc                                                             <  8:249179/148‑1 (MQ=255)                                                                                       agagCCTTTAGGTGCCTCTCGTGGAACCGCCAGCGTGGACGGTACATATGCCTGGCCCCATTCGAACCGGGCCTGGCGTTTCATGATCAGGTCA‑AATCGTTCCTGCGGTTTCATCCAAGCTTTCCAAtct                                                                             >  6:71973/1‑130 (MQ=255)                                                                                       agagCCTTTAGGTGCCTCTCGTGGAACCGCCAGCGTGGACGGTACATATGCCTGGCCCCATTCGAACCGGGCCTGGCGTTTCATGATCAGGTCA‑AATCGTTCCTGCGGTTTCATCCAAGCTTTCCAAtct                                                                             <  5:71973/130‑1 (MQ=255)                                                                                           ccTTTAGGTGCCTCTCGTGGAACCGCCAGCGTGGACGGTACATATGCCTGGCCCCATTCGAACCGGGCCTGGCGTTTCATGATCAGGTCA‑AATCGTTCCTGCGGTTTCATCCAAGCTTTCCAATCTCACTAGGATTTCCTGCCGAAGGc                                                      <  6:148522/149‑1 (MQ=255)                                                                                             tttAGGTGCCTCTCGTGGAACCGCCAGCGTGGACGGAACATATGCCTGGCCCCATTCGAACCGGGCCTGGCGTTTCATGATCAGGTCA‑AATCGTTCCTGCGGTTTCATCCAAGCTTTCCAATCTCACTAGGATTTCCTGCCGAAGGCAc                                                    >  5:102631/1‑149 (MQ=255)                                                                                                 ggTGCCTCTCGTGGAACCGCCAGCGTGGACGGTACATATGCCTGGCCCCATTCGAACCGGGCCTGGCGTTTCATGATCAGGTCA‑AATCGTTCCTGCGGTTTCATCCAAGCTTTCCAATCTCACTAGGATTTCCTGCCGAAGGCACGTTg                                                >  1:235814/1‑149 (MQ=255)                                                                                                  gTGCCTCTCGTGGAACCGCCAGCGTGGACGGTACATATGCCTGGCCCCATTCGAACCGGGCCTGGCg                                                                                                                                  <  5:280806/67‑1 (MQ=255)                                                                                                  gTGCCTCTCGTGGAACCGCCAGCGTGGACGGTACATATGCCTGGCCCCATTCGAACCGGGCCTGGCg                                                                                                                                  >  6:280806/1‑67 (MQ=255)                                                                                                   tGCCTCTCGTGGAACCGCCAGCGTGGACGGTACATATGCCTGGCCCCATTCGAACCGGGCCTGGCGTTTCATGATCAGGTCA‑AATCGTTCCTGCGGTTTCATCCAAGCTTTCCAATCTCACTAGGATTTCCTGCCGAAGGCACGTTggg                                              <  6:215274/149‑1 (MQ=255)                                                                                                     ccTCTCGTGGAACCGCCAGCGTGGACGGTACATATGCCTGGCCCCATTCGAACCGGGCCTGGCGTTTCATGATCAGGTCA‑AATCGTTCCTGCGGTTTCATCCAAGCTTTCCAATCTCACTAGGATTTCCTGCCGAAGGCACGTTGGGGa                                            <  1:292222/149‑1 (MQ=255)                                                                                                      ctctCGTGGAACCGCCAGCGTGGACGGTACATATGCCTGGCCCCATTCGAACCGGGCCTGGCGTTTCATGATCAGGTCA‑AATCGTTCCTGCGGTTTCATCCAAGCTTTCCAATCTCACTAGGATTTCCTGCCGaa                                                         <  6:205568/135‑1 (MQ=255)                                                                                                      ctctCGTGGAACCGCCAGCGTGGACGGTACATATGCCTGGCCCCATTCGAACCGGGCCTGGCGTTTCATGATCAGGTCA‑AATCGTTCCTGCGGTTTCATCCAAGCTTTCCAATCTCACTAGGATTTCCTGCAGaa                                                         >  5:205568/1‑135 (MQ=255)                                                                                                       tctcGTGGAACCGCCAGCGTGGATGGTACATATGCCTGGCCCCATTCGAACCGGGCCTGGCGTTTCATGATCAGGTCA‑AATCGTTCCTGCGGTTTCATCCAAGCTTTCCAATCTCACTAGGATTTCCTGCCGAAGGCACGTTgggg                                             <  7:27916/146‑1 (MQ=255)                                                                                                       tctcGTGGAACCGCCAGCGTGGATGGTACATATGCCTGGCCCCATTCGAACCGGGCCTGGCGTTTCATGATCAGGTCA‑AATCGTTCCTGCGGTTTCATCCAAGCTTTCCAATCTCACTAGGATTTCCTGCCGAAGGCACGTTgggg                                             >  8:27916/1‑146 (MQ=255)                                                                                                       tctcGTGGAACCGCCAGCGTGGACGGTACATATGCCTGGCCCCATTCGAACCGGGCCTGGCGTTTCATGATCAGGTCA‑AATCGTTCCTGCGGTTTCATCCAAGCTTTCCAATCTCACTAGGATTTCCTGCCGAAGGCACGTTGGGGAt                                           >  3:77311/1‑148 (MQ=255)                                                                                                        ctcGTGGAACCGCCAGCGTGGACGGTACATATGCCTGGCCCCATTCGAACCGGGCCTGGCGTTTCATGATCAGGTCA‑AATCGTTCCTGCGGTTTCATCCAAGCTTTCCAATCTCACTAGGATTTCCTGCCGAAGGCACGTTGGGGATaa                                         >  5:282366/1‑149 (MQ=255)                                                                                                            tGGAACCGCCAGCGTGGACGGTACATATGCCTGGCCCCATTCGAACCGGGCCTGGCGTTTCATGATCAGGTCA‑AATCGTTCCTGCGGTTTCATCCAAGCTTTCCAATCTCACTAGGATTTCCTGCCGAAGGCACGTTGGGGATaacaa                                      >  1:341977/1‑148 (MQ=255)                                                                                                              gAACCGCCAGCGTGGACGGTACATATGCCTGGCGCCATTCGAACCGGGACTGGCGTTTAATGATCAGGTCA‑AATCGTTCCTGCGGTTTCATCCAAGATTTCCAATCTCACTACGATTTCCTGCCGAAGGCACGTTGGGGATAAcaccac                                   >  6:86092/1‑149 (MQ=255)                                                                                                                    ccAGCGTGGACGGTACATATGCCTGGCCCCATTCGAACTGGGCCTGGCGTTTCATGATCAGGTCA‑AATCGTTCCTGCGGTTTCATCCAAGCTTTCCAATCTCACTAGGATTTCCTGCCGAAGGCACGTTGGGGATAACAACACGCacac                             >  8:153712/1‑149 (MQ=255)                                                                                                                    ccAGCGTGGACGGTACATATGCCTGGCCCCATTCGAACCGGGCCTGGCGTTTCATGATCAGGTCA‑AATCGTTCCTGCGGTTTCATCCAAGCTTTCCAATCTCACTAGGATTTCCt                                                               <  2:239207/115‑1 (MQ=255)                                                                                                                    ccAGCGTGGACGGTACATATGCCTGGCCCCATTCGAACCGGGCCTGGCGTTTCATGATCAGGTCA‑AATCGTTCCTGCGGTTTCATCCAAGCTTTCCAATCTCACTAGGATTTCCt                                                               >  1:239207/1‑115 (MQ=255)                                                                                                                    ccAGCGTGGACGGTACATATGCCTGGCCCCATTCGAACCGGGCCTGGCGTTTCATGATCAGGTCA‑AATCGTTCCTGCGGTTTCATCCAAGCTTTCCAATCTCACTAGGATTTCCTGCCGAAGGCACGTTGGGGAt                                           <  8:16758/135‑1 (MQ=255)                                                                                                                    ccAGCGTGGACGGTACATATGCCTGGCCCCATTCGAACCGGGCCTGGCGTTTCATGATCAGGTCA‑AATCGTTCCTGCGGTTTCATCCAAGCTTTCCAATCTCACTAGGATTTCCTGCCGAAGGCACGTTGGGGAt                                           >  7:16758/1‑135 (MQ=255)                                                                                                                    ccAGCGTGGACGGTACATATGCCTGGCCCCATTCGAACCGGGCCTGGCGTTTCATGATCAGGTCA‑AATCGTTCCTGCGGTTTCATCCAAGCTTTCCAATCTCACTAGGATTTCCTGCCGAAGGCACGTTGGGGATACCAACACGCacac                             >  2:268344/1‑149 (MQ=255)                                                                                                                    ccAGCGTGGACGGTACATATGCCTGGCCCCATTCGAACCGGGCCTGGCGTTTCATGATCAGGTCA‑AATCGTTCCTGCGGTTTCATCCAAGCTTTCCAATCTCACTAGGATTTCCTGCCGAAGGCACGTTGGGGATAACAACACGCacac                             >  2:272991/1‑149 (MQ=255)                                                                                                                     cAGCGTGGACGGTACATATGCCTGGCCCCATTCGAACCGGGCCTGGCGTTTCATGATCAGGTCA‑AATCGTTCCTGCGGTTTCATCCAAGCTTTCCAATCTCACTAGGATTTCCTGCCGAAGGCACGTTGGGGATAACAACACGCACAcc                            >  4:59791/1‑149 (MQ=255)                                                                                                                              cGGTACATATGCCTGGCCCCATTCGAACCGGGCCTGGCGTTTCATGATCAGGTCA‑AATCGTTCCTGCGGTTTCATCCAAGCTTTCCAATCTCACTAGGATTTCCTGCCGAAGGCACGTTGGGGATAACAACACGCACACCGGCAAAAcc                   <  4:77311/149‑1 (MQ=255)                                                                                                                              cGGTACATATGCCTGGCCCCATTCGAACCGGGCCTGGCGTTTCATGATCAGGTCA‑AATCGTTCCTGCGGTTTCATCCAAGCTTTCCAATCTCAATAGGATTTCCTGCCGAAGGCACGTTGGGGATAACAACACGCACACCGGCAAAAcc                   <  2:341977/149‑1 (MQ=255)                                                                                                                                gTACATATGCCTGGCCCCATTCGAACCGGGCCTGGCGTTTCATGATCAGGTCA‑AATCGTTCCTGCGGTTTCATCCAAGCTTTCCAATCTCACTAGGATTTCCTGCCGAAGGCACGTTGGGGATAACAACACGCACACCGGCAAAACCCt                 >  8:192249/1‑149 (MQ=255)                                                                                                                                 tACATATGCCTGGCCCCATTCGAACTGGGCCTGGCGTTTCATGATCAGGTCA‑AATCGTTCCTGCGGTTTCATCCAAGCTTTCCAATCTCACTAGGATTTCCTGCCGAAGGCACGTTGGGGATAACAACACGCACACCGGCAAAACCCtt                <  7:153712/149‑1 (MQ=255)                                                                                                                                 tACATATGCCTGGCCCCATTCGAACCGGGCCTGGCGTTTCATGATCAGGTCA‑AATCGTTCCTGCGGTTTCATCCAAGCTTTCCAATCTCACTAGGATTTCCTGCCGAAGGCACGTTGGGGATAACAACACGCACACCGGCAAAACCCt                 >  3:121809/1‑148 (MQ=255)                                                                                                                                    atatGCCTGGCCCCATTCGAACCGGGCCTGGCGTTTCATGATCAGGTCA‑AATCGTTCCTGCGGTTTCATCCAAGCTTTCCAATCTCACTAGGATTTCCTGCCGAAGGCACGTTGGGGATAACAACACGCACACCGGCAAAACCCtt                >  7:175642/1‑146 (MQ=255)                                                                                                                                    atatGCCTGGCCCCATTCGAACCGGGCCTGGCGTTTCATGATCAGGTCA‑AATCGTTCCTGCGGTTTCATCCAAGCTTTCCAATCTCACTAGGATTTCCTGCCGAAGGCACGTTGGGGATAACAACACGCACACCGGCAAAACCCtt                <  8:175642/146‑1 (MQ=255)                                                                                                                                    atatGCCTGGCCCCATTCGAACCGGGCCTGGCGTTTCATGATCAGGTCA‑AATCGTTCCTGCGGTTTCATCCAAGCTTTCCAATCTCACTAGGATTTCCTGCCGAAGGCACGTTGGGGATAACAACACGCACACCGGCAAAACCCTTATc             >  3:293951/1‑149 (MQ=255)                                                                                                                                      atGCCTGGCCCCATTCGAACCGGGCCTGGCGTTTCATGATCAGGTCACAATCGTTCCTGCGGTTTCATCCAAGCTTTCCAATCTCACTAGGATTTCCTGCCGAAGGCACGTTGGGGATAACAACACGCACACCGGCAAAACCCTTATcc            >  1:79225/1‑149 (MQ=255)                                                                                                                                      atGCCTGGCCCCATTCGAACCGGGCCTGGCGTTTCATGATCAGGTCA‑AATCGTTCCTGCGGTTTCATCCAAGCTTTCCAATCTCACTAGGATTTCCTGCCGAAGGCACGTTGGGGATAACAACACGCACACCGGCAAAACCCTTATCCt           >  3:235403/1‑149 (MQ=255)                                                                                                                                         ccTGGCCCCATTCGAACCGGGCCTGGCGTTTCATGATCAGGTCA‑AATCGTTCCTGCGGTTTCATCCAAGCTTTCCAATCTCACCAGGATTTCCTGCCGAAGGCACGTTGGGGATAACAACACGCACACCGGCAAAACCCTTATCCTGCg        <  8:47187/149‑1 (MQ=255)                                                                                                                                         ccTGGCCCCATTCGAACCGGGCCTGGCGTTTCATGATCAGGTCA‑AATCGTTCCTGCGGTTTCATCCAAGCTTTCCAATCTCACCAGGATTTCCTGCCGAAGGCACGTTGGGGATAACAACACGCACACCGGCAAAACCCTTATCCTGCg        <  8:52220/149‑1 (MQ=255)                                                                                                                                          cTGGCCCCATTCGAACCGGGCCTGGCGTTTCATGATCAGGTCA‑AATCGTTCCTGCGGTTTCATCCAAGCTTTCCAATCTCACTAGGATTTCCTGCCGAAGGCACGTTGGGGATAACAACACGCACACCGGCAAAACCCTTa               >  3:191693/1‑141 (MQ=255)                                                                                                                                          cTGGCCCCAATCGAACCGGGCCTGGCGTTTCATGATCAGGTCA‑AATCGTTCCTGCGGTTTCATCCAAGCTTTCCAATCTCACTAGGATTTCCTGCCGAAGGCACGTTGGGGATAACAACACGCACACCGGCAAAACCCTTa               <  4:191693/141‑1 (MQ=255)                                                                                                                                             gCCCCATTCGAACCGGGCCTGGCGTTTCATGATCAGGTCA‑AATCGTTCCTGCGGTTTCATCCAAGCTTTCCAATCTCACTAGGATTTCCTGCCGAAGGCACGTTGGGGATAACAACACGCACACGGGCAAAACCCTTATCCTGCGGGc     <  6:282366/148‑1 (MQ=255)                                                                                                                                              ccccATTCGAACCGGGCCTGGCGTTTCATGATCAGGTCA‑AATCGTTCCTGCGGTTTCATCCAAGCTTTCCAATCTCACTATGATTTCCTGCCGAAGGCACGTTGGTGATAACAACACGCACACCGGCAAAACCCTTATCCTGCGGGTAg   <  2:235814/149‑1 (MQ=255)                                                                                                                                              ccccATTCGAACCGGGCCTGGCGTTTCATGATCAGGTCA‑AATCGTTCCTGCGGTTTCATCCAAGCTTTCCAATCTCACTAGGATTTCCTGCCGAAGGCACGTTGGGGATAACAACACGCACACCGGCAAAACCCTTATCCTGCGGGCAg   <  7:331875/149‑1 (MQ=255)                                                                                                                                               cccATTCGAACCGGGCCTGGCGTTTCATGATCAGGTCA‑AATCGTTCCTGCGGTTTCATCCAAGCTTTCCAATCTCACTAGGATTTCCTGCCGAAGGCACGTTGGGGATAACAACACGCACACCGGCAAAACCCTTATCCTGCGGGCAgg  >  6:352191/1‑149 (MQ=255)                                                                                                                                                ccATTCGAACCGGGCCTGGCGTTTCATGATCAGGTCA‑AATCGTTCCTGCGGTTTCATCCAAGCTTTCCAATCTCACTAGGATTTCCTGCCGAAGGCACGTTGGGGATAACAACACGCACACCGGCAAAACCCTTATCCTGCGGGCAgg  >  6:350511/1‑148 (MQ=255)                                                                                                                                                 |                                                                                                                                                    AGAGCTAATTGCGCAAACACCTTCTCTGGGTTGGAAAGGAGATGAATTGTGCGCCCCAGCTTTCGACTATGCAGTCTGCATATGCGAGAGCCTTTAGGTGCCTCTCGTGGAACCGCCAGCGTGGACGGTACATATGCCTGGCCCCATTCGAACCGGGCCTGGCGTTTCATGATCAGGTCA‑AATCGTTCCTGCGGTTTCATCCAAGCTTTCCAATCTCACTAGGATTTCCTGCCGAAGGCACGTTGGGGATAACAACACGCACACCGGCAAAACCCTTATCCTGCGGGCAGG  >  NC_002947/6166042‑6166332

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑