Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	6,166,195	C→G	5.3%	R16P (CGG→CCG)	PP_5406 ←	transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	6,166,195	0	C	G	5.3%	239.5 / 3.6	77	R16P (CGG→CCG)	PP_5406	transposase
Reads supporting (aligned to +/- strand):  ref base C (41/30);  new base G (2/2);  total (44/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.62e-01

AAACACCTTCTCTGGGTTGGAAAGGAGATGAATTGTGCGCCCCAGCTTTCGACTATGCAGTCTGCATATGCGAGAGCCTTTAGGTGCCTCTCGTGGAACCGCCAGCGTGGACGGTACATATGCCTGGCCCCATTCGAACCGGGCCTGGCGTTTCATGATCAGGTCA‑AATCGTTCCTGCGGTTTCATCCAAGCTTTCCAATCTCACTAGGATTTCCTGCCGAAGGCACGTTGGGGATAACAACACGCACACCGGCAAAACCCTTATCCTGCGGGCAGGTAACCGCG  >  NC_002947/6166056‑6166340                                                                                                                                            |                                                                                                                                                   aaaCACCTTCTCTGGGTTGGAAAGGAGATGAATTGTGCGCCCCAGCTTTCGACTATGCAGTCTGCATATGCGAGAGCCTTTAGGTGCCTCTCGTGGAACCGCCAGCGTGGACGGTACATATGCCTGGCCCCATTCGAACCgg                                                                                                                                                  <  5:217614/142‑1 (MQ=255) aaaCACCTTCTCTGGGTTGGAAAGGAGAAGAATTGTGCGCCCCAGCTTTCGACTATGCAGTCTGCATATGCGAGAGCCTTTAGGTGCCTCTCGTGGAACCGCCACCGTGGACGGTACATATGCCTGGCCCCATTCGAACCgg                                                                                                                                                  >  6:217614/1‑142 (MQ=255)       cTTCTCTGGGTTGGAAAGGAGATGAATTGTGCGCCCCAGCTTTCGACTATGCAGTCTGCATATGCGAGAGCCTTTAGGTGCCTCTCGTGGAACCGCCAGCGTGGACGGTACATATGCCTGGCCCCATTCGAACCGGGCCTGGCGTTTCa                                                                                                                                     >  8:331875/1‑149 (MQ=255)                   ggAAAGGAGATGAATTGTGCGCCCCAGCTTTCGACTATGCAGTCTGCATATGCGAGAGCCTTTAGGTGCCTCTCGTGGAACCGCCAGCGTGGACGGTACATATGCCTGGCCCCATTCGAACCGGGCCTGGCGTTTCATGATCAGGTCa                                                                                                                          >  5:189597/1‑148 (MQ=255)                    gAAAGGAGATGAATTGTGCGCCCCAGCTTTCGACTATGCAGTCTGCATATGCGAGAGCCTTTAGGTGCCTCTCGTGGAACCGCCAGCGTGGACGGTACATATGCCTGGCCCCATTCGAACCGGGCCTGGCGTTTCATGATCAGGTCa‑aa                                                                                                                       >  4:161110/1‑149 (MQ=255)                      aaGGAGATGAATTGTGCGCCCCAGCTTTCGACTATGCAGTCTGCATATGCGAGAGCCTTTAGGTGCCTCTCGTGGAACCGCCAGCGTGGACGGTACATATGCCTGGCCCCATTCGAACCGGGCCTGGCGTTTCATGATCAGGTCA‑AATc                                                                                                                     <  5:65256/149‑1 (MQ=255)                      aaGGAGATGAATTGTGCGCCCCAGCTTTCGACTATGCAGTCTGCATATGCGAGAGCCTTTAGGTGCCTCTCGTGGAACCGCCAGCGTGGACGGTACATATGCCTGGCCCCATTCGAACCGGGCCTGGCGTTTCATGATCAGGTCA‑AATc                                                                                                                     <  7:182828/149‑1 (MQ=255)                        ggAGATGAATTGTGCGCCCCAGCTTTCGACTATGCAGTCTGCATATGCGAGAGCCTTTAGGTGCCTCTCGTGGAACCGCCAGCGTGGACGGTACATATGCCTGGCCCCATTCGAACCGGGCCTGGCGTTTCATGATCAGGTCA‑AATCGt                                                                                                                   <  6:189597/149‑1 (MQ=255)                           gaTGAATTGTGCGCCCCAGCTTTCGACTATGCAGTCTGCATATGCGAGAGCCTTTAGGTGCCTCTCGTGGAACCGCCAGCGTGGACGGTACATATGCCTGGCCCCATTCGAACCGGGCCTGGCGTTTCa                                                                                                                                     <  8:253773/129‑1 (MQ=255)                           gaTGAATTGTGCGCCCCAGCTTTCGACTATGCAGTCTGCATATGCGAGAGCCTTTAGGTGCCTCTCGTGGAACCGCCAGCGTGGACGGTACATATGCCTGGCCCCATTCGAACCGGGCCTGGCGTTTCa                                                                                                                                     >  7:253773/1‑129 (MQ=255)                               aaTTGTGCGCCCCAGCTTTCGACTATGCAGTCTGCATATGCGAGAGCCTTTAGGTGCCTCTCGTGGAACCGCCAGCGTGGACGGTACATATGCCTGGCCCCATTCGAACCGGGCCTGGCGTTTCATGATCAGGTCA‑AATCGTTCCTGCg                                                                                                            >  4:259602/1‑149 (MQ=255)                                     gcgcCCCAGCTTTCGACTATGCAGTCTGCATATGCGAGAGCCTTTAGGTGCCTCTCGTGGAACCGCCAGCGTGGACGGTACATATGCCTGGCCCCATTCGAACCGGGCCTGGCGTTTCATGATCAGGTCA‑AATCGTTCCTGCGGTTTCa                                                                                                      >  3:152424/1‑149 (MQ=255)                                       gcCCCAGCTTTCGACTATGCAGTCTGCATATGCGAGAGCCTTTAGGTGCCTCTCGTGGAACCGCCAGCGTGGACGGTACATATGCCTGGCCCCATTCGAACCGGGCCTGGCGTTTCATGATCAGGTCA‑AATCGTTCCTGCGGTTTCATc                                                                                                    <  3:259602/149‑1 (MQ=255)                                          ccAGCTTTCGACTATGCAGTCTGCATATGCGAGAGCCTTTAGGTGCCTCTCGTGGAACCGCCAGCGTGGACGGTACATATGCCTGGCCCCATTCGAACCGGGCCTGGCGTTTCATGATCAGGTCA‑AATCGTTCCTGCGGTTTCATCCaa                                                                                                 <  3:161110/149‑1 (MQ=255)                                           cAGCTTTCGACTATGCAGTCTGCATATGCGAGAGCCTTTAGGTGCCTCTCGTGGAACCGCCAGCGTGGACGGTACATATGCCAGGCCCGGTTCGAATGGGGCCAgg                                                                                                                                            <  1:105028/106‑1 (MQ=38)                                           cAGCTTTCGACTATGCAGTCTGCATATGCGAGAGCCTTTAGGTGCCTCTCGTGGAACCGCCAGCGTGGACGGTACATATGCCAGGCCCGGTTCGAATGGGGCCAgg                                                                                                                                            >  2:100539/1‑106 (MQ=38)                                           cAGCTTTCGACTATGCAGTCTGCATATGCGAGAGCCTTTAGGTGCCTCTCGTGGAACCGCCAGCGTGGACGGTACATATGCCAGGCCCGGTTCGAATGGGGCCAgg                                                                                                                                            >  2:105028/1‑106 (MQ=38)                                           cAGCTTTCGACTATGCAGTCTGCATATGCGAGAGCCTTTAGGTGCCTCTCGTGGAACCGCCAGCGTGGACGGTACATATGCCAGGCCCGGTTCGAATGGGGCCAgg                                                                                                                                            <  1:100539/106‑1 (MQ=38)                                            aGCTTTCGACTATGCAGTCTGCATATGCGAGAGCCTTTAGGTGCCTCTCGTGGAACCGCCAGCGTGGACGGTACATATGCCTGGCCCCATTCGAACCGGGCCTGGCGTTTCATGATCAGGTCA‑AATCGTTCCTGCGGTTTCATCCAAGc                                                                                               >  2:292222/1‑149 (MQ=255)                                                 tctaCTATGCAGTCTGCATATGCGAGAGCCTTTAGGTGCCTCTCGTGGAACCGACAGCGTGGACGGTACATATTCCTGTCCCCATTCGAACCGGGCCTGGCGTTTCATGATCAGGTCA‑AATCGTTCCTGCGGTTTCATCCAAGCTTTc                                                                                           <  5:283926/145‑1 (MQ=255)                                                 tCGACTATGCAGTCTGCATATGCGAGAGCCTTTAGGTGCCTCTCGTGGAACCGCCAGCGTGGACGGTACATATGCCTGGCCCCATTCGAACCGGGCCTGGCGTTTCATGATCAGGTCA‑AATCGTTc                                                                                                                 <  8:105391/126‑1 (MQ=255)                                                 tCGACTATGCAGTCTGCATATGCGAGAGCCTTTAGGTGCCTCTCGTGGAACCGCCAGCGTGGACGGTACATATGCCTGGCCCCATTCGAACCGGGCCTGGCGTTTCATGATCAGGTCA‑AATCGTTc                                                                                                                 >  7:105391/1‑126 (MQ=255)                                                   gACTATGCAGTCTGCATATGCGAGAGCCTTTAGGTGCCTCTCGTGGAACCGCCAGCGTGGACGGTACATATGCCTGGCCCCATTCGAACCGGGCCTGGCGTTTCATGATCAGGTCA‑AATCGTTCCTGCGGTTTCATCCAAGCTTTCCaa                                                                                        >  5:148522/1‑149 (MQ=255)                                                    aCTATGCAGTCTGCATATGCGAGAGCCTTTAGGTGCCTCTCGTGGAACCGCCAGCGTGGACGGTACATATGCCTGGCCCCATTCGAACCGGGCCTGGCGTTTCATGATCAGGTCA‑AATCGTTCCTGCGGTTTCATCCAAGCTTTCCAAt                                                                                       >  7:52220/1‑149 (MQ=255)                                                    aCTATGCAGTCTGCATATGCGAGAGCCTTTAGGTGCCTCTCGTGGAACCGCCAGCGTGGACGGTACATATGCCTGGCCCCATTCGAACCGGGCCTGGCGTTTCATGATCAGGTCA‑AATCGTTCCTGCGGTTTCATCCAAGCTTTCCAAt                                                                                       >  7:47187/1‑149 (MQ=255)                                                           aGTCTGCATATGCGAGAGCCTTTAGGTGCCTCTCGTGGAACCGCCAGCGTGGACGGTACATATGCCTGGCCCCATTCGAACCGGGCCTGGCGTTTCATGATCAGGTCA‑AATCGTTCCTGCGGTTTCATCCAAGCTTTCCAATCTCAc                                                                                  >  5:50991/1‑147 (MQ=255)                                                           aGTCTGCATATGCGAGAGCCTTTAGGTGCCTCTCGTGGAACCGCCAGCGTGGACGGTACATATGCCTGGCCCCATTCGAACCGGGCCTGGCGTTTCATGATCAGGTCA‑AATCGTTCCTGCGGTTTCATCCAAGCTTTCCAATCTCACTa                                                                                <  4:152424/149‑1 (MQ=255)                                                                  atatGCGAGAGCCTTTAGGTGCCTCTCGTGGAACCGCCAGCGTGGACGGTACATATGCCTGGCCCCATTCGAACCGGGCCTGGCGTTTCATGATCa                                                                                                                               >  4:119888/1‑96 (MQ=255)                                                                  atatGCGAGAGCCTTTAGGTGCCTCTCGTGGAACCGCCAGCGTGGACGGTACATATGCCTGGCCCCATTCGAACCGGGCCTGGCGTTTCATGATCa                                                                                                                               <  3:119888/96‑1 (MQ=255)                                                                  atatGCGAGAGCCTTTAGGTGCCTCTCGTGGAACCGCCAGCGTGGACGGTACATATGCCTGGCCCCATTCGAACCGGGCCTGGCGTTTCATGATCAGGTCA‑AATCGTTCCTGCGGTTTCATCCAAGCTTTCCAATCTCACTAGGATTTc                                                                         >  6:161365/1‑149 (MQ=255)                                                                     tGCGAGAGCCTTTAGGTGCCTCTCGTGGAACCGCCAGCGTGGACGGTACATATGCCTGGCCCCATTCGAACCGGGCCTGGCGTTTCATGATCAGGTCA‑AATCGTTCCTGCGGTTTCATCCAAGCTTTCCAATCTCACTAGGATTTCCTg                                                                      >  5:215274/1‑149 (MQ=255)                                                                       cGAGAGCCTTTAGGTGCCTCTCGTGGAACCGCCAGCGTGGACGGTACATATGCCTGGCCCCATTCGAACCGGGCCTGGCGTTTCATGATCAGGTCA‑AATCGTTCCTGCGGTTTCATCCAAGCTTTCCAATCTCACTAGGATTTCCTGc                                                                     <  8:249179/148‑1 (MQ=255)                                                                         agagCCTTTAGGTGCCTCTCGTGGAACCGCCAGCGTGGACGGTACATATGCCTGGCCCCATTCGAACCGGGCCTGGCGTTTCATGATCAGGTCA‑AATCGTTCCTGCGGTTTCATCCAAGCTTTCCAAtct                                                                                     <  5:71973/130‑1 (MQ=255)                                                                         agagCCTTTAGGTGCCTCTCGTGGAACCGCCAGCGTGGACGGTACATATGCCTGGCCCCATTCGAACCGGGCCTGGCGTTTCATGATCAGGTCA‑AATCGTTCCTGCGGTTTCATCCAAGCTTTCCAAtct                                                                                     >  6:71973/1‑130 (MQ=255)                                                                             ccTTTAGGTGCCTCTCGTGGAACCGCCAGCGTGGACGGTACATATGCCTGGCCCCATTCGAACCGGGCCTGGCGTTTCATGATCAGGTCA‑AATCGTTCCTGCGGTTTCATCCAAGCTTTCCAATCTCACTAGGATTTCCTGCCGAAGGc                                                              <  6:148522/149‑1 (MQ=255)                                                                               tttAGGTGCCTCTCGTGGAACCGCCAGCGTGGACGGAACATATGCCTGGCCCCATTCGAACCGGGCCTGGCGTTTCATGATCAGGTCA‑AATCGTTCCTGCGGTTTCATCCAAGCTTTCCAATCTCACTAGGATTTCCTGCCGAAGGCAc                                                            >  5:102631/1‑149 (MQ=255)                                                                                   ggTGCCTCTCGTGGAACCGCCAGCGTGGACGGTACATATGCCTGGCCCCATTCGAACCGGGCCTGGCGTTTCATGATCAGGTCA‑AATCGTTCCTGCGGTTTCATCCAAGCTTTCCAATCTCACTAGGATTTCCTGCCGAAGGCACGTTg                                                        >  1:235814/1‑149 (MQ=255)                                                                                    gTGCCTCTCGTGGAACCGCCAGCGTGGACGGTACATATGCCTGGCCCCATTCGAACCGGGCCTGGCg                                                                                                                                          >  6:280806/1‑67 (MQ=255)                                                                                    gTGCCTCTCGTGGAACCGCCAGCGTGGACGGTACATATGCCTGGCCCCATTCGAACCGGGCCTGGCg                                                                                                                                          <  5:280806/67‑1 (MQ=255)                                                                                     tGCCTCTCGTGGAACCGCCAGCGTGGACGGTACATATGCCTGGCCCCATTCGAACCGGGCCTGGCGTTTCATGATCAGGTCA‑AATCGTTCCTGCGGTTTCATCCAAGCTTTCCAATCTCACTAGGATTTCCTGCCGAAGGCACGTTggg                                                      <  6:215274/149‑1 (MQ=255)                                                                                       ccTCTCGTGGAACCGCCAGCGTGGACGGTACATATGCCTGGCCCCATTCGAACCGGGCCTGGCGTTTCATGATCAGGTCA‑AATCGTTCCTGCGGTTTCATCCAAGCTTTCCAATCTCACTAGGATTTCCTGCCGAAGGCACGTTGGGGa                                                    <  1:292222/149‑1 (MQ=255)                                                                                        ctctCGTGGAACCGCCAGCGTGGACGGTACATATGCCTGGCCCCATTCGAACCGGGCCTGGCGTTTCATGATCAGGTCA‑AATCGTTCCTGCGGTTTCATCCAAGCTTTCCAATCTCACTAGGATTTCCTGCCGaa                                                                 <  6:205568/135‑1 (MQ=255)                                                                                        ctctCGTGGAACCGCCAGCGTGGACGGTACATATGCCTGGCCCCATTCGAACCGGGCCTGGCGTTTCATGATCAGGTCA‑AATCGTTCCTGCGGTTTCATCCAAGCTTTCCAATCTCACTAGGATTTCCTGCAGaa                                                                 >  5:205568/1‑135 (MQ=255)                                                                                         tctcGTGGAACCGCCAGCGTGGATGGTACATATGCCTGGCCCCATTCGAACCGGGCCTGGCGTTTCATGATCAGGTCA‑AATCGTTCCTGCGGTTTCATCCAAGCTTTCCAATCTCACTAGGATTTCCTGCCGAAGGCACGTTgggg                                                     <  7:27916/146‑1 (MQ=255)                                                                                         tctcGTGGAACCGCCAGCGTGGATGGTACATATGCCTGGCCCCATTCGAACCGGGCCTGGCGTTTCATGATCAGGTCA‑AATCGTTCCTGCGGTTTCATCCAAGCTTTCCAATCTCACTAGGATTTCCTGCCGAAGGCACGTTgggg                                                     >  8:27916/1‑146 (MQ=255)                                                                                         tctcGTGGAACCGCCAGCGTGGACGGTACATATGCCTGGCCCCATTCGAACCGGGCCTGGCGTTTCATGATCAGGTCA‑AATCGTTCCTGCGGTTTCATCCAAGCTTTCCAATCTCACTAGGATTTCCTGCCGAAGGCACGTTGGGGAt                                                   >  3:77311/1‑148 (MQ=255)                                                                                          ctcGTGGAACCGCCAGCGTGGACGGTACATATGCCTGGCCCCATTCGAACCGGGCCTGGCGTTTCATGATCAGGTCA‑AATCGTTCCTGCGGTTTCATCCAAGCTTTCCAATCTCACTAGGATTTCCTGCCGAAGGCACGTTGGGGATaa                                                 >  5:282366/1‑149 (MQ=255)                                                                                              tGGAACCGCCAGCGTGGACGGTACATATGCCTGGCCCCATTCGAACCGGGCCTGGCGTTTCATGATCAGGTCA‑AATCGTTCCTGCGGTTTCATCCAAGCTTTCCAATCTCACTAGGATTTCCTGCCGAAGGCACGTTGGGGATaacaa                                              >  1:341977/1‑148 (MQ=255)                                                                                                gAACCGCCAGCGTGGACGGTACATATGCCTGGCGCCATTCGAACCGGGACTGGCGTTTAATGATCAGGTCA‑AATCGTTCCTGCGGTTTCATCCAAGATTTCCAATCTCACTACGATTTCCTGCCGAAGGCACGTTGGGGATAAcaccac                                           >  6:86092/1‑149 (MQ=255)                                                                                                      ccAGCGTGGACGGTACATATGCCTGGCCCCATTCGAACTGGGCCTGGCGTTTCATGATCAGGTCA‑AATCGTTCCTGCGGTTTCATCCAAGCTTTCCAATCTCACTAGGATTTCCTGCCGAAGGCACGTTGGGGATAACAACACGCacac                                     >  8:153712/1‑149 (MQ=255)                                                                                                      ccAGCGTGGACGGTACATATGCCTGGCCCCATTCGAACCGGGCCTGGCGTTTCATGATCAGGTCA‑AATCGTTCCTGCGGTTTCATCCAAGCTTTCCAATCTCACTAGGATTTCCt                                                                       >  1:239207/1‑115 (MQ=255)                                                                                                      ccAGCGTGGACGGTACATATGCCTGGCCCCATTCGAACCGGGCCTGGCGTTTCATGATCAGGTCA‑AATCGTTCCTGCGGTTTCATCCAAGCTTTCCAATCTCACTAGGATTTCCt                                                                       <  2:239207/115‑1 (MQ=255)                                                                                                      ccAGCGTGGACGGTACATATGCCTGGCCCCATTCGAACCGGGCCTGGCGTTTCATGATCAGGTCA‑AATCGTTCCTGCGGTTTCATCCAAGCTTTCCAATCTCACTAGGATTTCCTGCCGAAGGCACGTTGGGGAt                                                   <  8:16758/135‑1 (MQ=255)                                                                                                      ccAGCGTGGACGGTACATATGCCTGGCCCCATTCGAACCGGGCCTGGCGTTTCATGATCAGGTCA‑AATCGTTCCTGCGGTTTCATCCAAGCTTTCCAATCTCACTAGGATTTCCTGCCGAAGGCACGTTGGGGAt                                                   >  7:16758/1‑135 (MQ=255)                                                                                                      ccAGCGTGGACGGTACATATGCCTGGCCCCATTCGAACCGGGCCTGGCGTTTCATGATCAGGTCA‑AATCGTTCCTGCGGTTTCATCCAAGCTTTCCAATCTCACTAGGATTTCCTGCCGAAGGCACGTTGGGGATACCAACACGCacac                                     >  2:268344/1‑149 (MQ=255)                                                                                                      ccAGCGTGGACGGTACATATGCCTGGCCCCATTCGAACCGGGCCTGGCGTTTCATGATCAGGTCA‑AATCGTTCCTGCGGTTTCATCCAAGCTTTCCAATCTCACTAGGATTTCCTGCCGAAGGCACGTTGGGGATAACAACACGCacac                                     >  2:272991/1‑149 (MQ=255)                                                                                                       cAGCGTGGACGGTACATATGCCTGGCCCCATTCGAACCGGGCCTGGCGTTTCATGATCAGGTCA‑AATCGTTCCTGCGGTTTCATCCAAGCTTTCCAATCTCACTAGGATTTCCTGCCGAAGGCACGTTGGGGATAACAACACGCACAcc                                    >  4:59791/1‑149 (MQ=255)                                                                                                                cGGTACATATGCCTGGCCCCATTCGAACCGGGCCTGGCGTTTCATGATCAGGTCA‑AATCGTTCCTGCGGTTTCATCCAAGCTTTCCAATCTCACTAGGATTTCCTGCCGAAGGCACGTTGGGGATAACAACACGCACACCGGCAAAAcc                           <  4:77311/149‑1 (MQ=255)                                                                                                                cGGTACATATGCCTGGCCCCATTCGAACCGGGCCTGGCGTTTCATGATCAGGTCA‑AATCGTTCCTGCGGTTTCATCCAAGCTTTCCAATCTCAATAGGATTTCCTGCCGAAGGCACGTTGGGGATAACAACACGCACACCGGCAAAAcc                           <  2:341977/149‑1 (MQ=255)                                                                                                                  gTACATATGCCTGGCCCCATTCGAACCGGGCCTGGCGTTTCATGATCAGGTCA‑AATCGTTCCTGCGGTTTCATCCAAGCTTTCCAATCTCACTAGGATTTCCTGCCGAAGGCACGTTGGGGATAACAACACGCACACCGGCAAAACCCt                         >  8:192249/1‑149 (MQ=255)                                                                                                                   tACATATGCCTGGCCCCATTCGAACTGGGCCTGGCGTTTCATGATCAGGTCA‑AATCGTTCCTGCGGTTTCATCCAAGCTTTCCAATCTCACTAGGATTTCCTGCCGAAGGCACGTTGGGGATAACAACACGCACACCGGCAAAACCCtt                        <  7:153712/149‑1 (MQ=255)                                                                                                                   tACATATGCCTGGCCCCATTCGAACCGGGCCTGGCGTTTCATGATCAGGTCA‑AATCGTTCCTGCGGTTTCATCCAAGCTTTCCAATCTCACTAGGATTTCCTGCCGAAGGCACGTTGGGGATAACAACACGCACACCGGCAAAACCCt                         >  3:121809/1‑148 (MQ=255)                                                                                                                      atatGCCTGGCCCCATTCGAACCGGGCCTGGCGTTTCATGATCAGGTCA‑AATCGTTCCTGCGGTTTCATCCAAGCTTTCCAATCTCACTAGGATTTCCTGCCGAAGGCACGTTGGGGATAACAACACGCACACCGGCAAAACCCtt                        >  7:175642/1‑146 (MQ=255)                                                                                                                      atatGCCTGGCCCCATTCGAACCGGGCCTGGCGTTTCATGATCAGGTCA‑AATCGTTCCTGCGGTTTCATCCAAGCTTTCCAATCTCACTAGGATTTCCTGCCGAAGGCACGTTGGGGATAACAACACGCACACCGGCAAAACCCtt                        <  8:175642/146‑1 (MQ=255)                                                                                                                      atatGCCTGGCCCCATTCGAACCGGGCCTGGCGTTTCATGATCAGGTCA‑AATCGTTCCTGCGGTTTCATCCAAGCTTTCCAATCTCACTAGGATTTCCTGCCGAAGGCACGTTGGGGATAACAACACGCACACCGGCAAAACCCTTATc                     >  3:293951/1‑149 (MQ=255)                                                                                                                        atGCCTGGCCCCATTCGAACCGGGCCTGGCGTTTCATGATCAGGTCACAATCGTTCCTGCGGTTTCATCCAAGCTTTCCAATCTCACTAGGATTTCCTGCCGAAGGCACGTTGGGGATAACAACACGCACACCGGCAAAACCCTTATcc                    >  1:79225/1‑149 (MQ=255)                                                                                                                        atGCCTGGCCCCATTCGAACCGGGCCTGGCGTTTCATGATCAGGTCA‑AATCGTTCCTGCGGTTTCATCCAAGCTTTCCAATCTCACTAGGATTTCCTGCCGAAGGCACGTTGGGGATAACAACACGCACACCGGCAAAACCCTTATCCt                   >  3:235403/1‑149 (MQ=255)                                                                                                                           ccTGGCCCCATTCGAACCGGGCCTGGCGTTTCATGATCAGGTCA‑AATCGTTCCTGCGGTTTCATCCAAGCTTTCCAATCTCACCAGGATTTCCTGCCGAAGGCACGTTGGGGATAACAACACGCACACCGGCAAAACCCTTATCCTGCg                <  8:47187/149‑1 (MQ=255)                                                                                                                           ccTGGCCCCATTCGAACCGGGCCTGGCGTTTCATGATCAGGTCA‑AATCGTTCCTGCGGTTTCATCCAAGCTTTCCAATCTCACCAGGATTTCCTGCCGAAGGCACGTTGGGGATAACAACACGCACACCGGCAAAACCCTTATCCTGCg                <  8:52220/149‑1 (MQ=255)                                                                                                                            cTGGCCCCATTCGAACCGGGCCTGGCGTTTCATGATCAGGTCA‑AATCGTTCCTGCGGTTTCATCCAAGCTTTCCAATCTCACTAGGATTTCCTGCCGAAGGCACGTTGGGGATAACAACACGCACACCGGCAAAACCCTTa                       >  3:191693/1‑141 (MQ=255)                                                                                                                            cTGGCCCCAATCGAACCGGGCCTGGCGTTTCATGATCAGGTCA‑AATCGTTCCTGCGGTTTCATCCAAGCTTTCCAATCTCACTAGGATTTCCTGCCGAAGGCACGTTGGGGATAACAACACGCACACCGGCAAAACCCTTa                       <  4:191693/141‑1 (MQ=255)                                                                                                                               gCCCCATTCGAACCGGGCCTGGCGTTTCATGATCAGGTCA‑AATCGTTCCTGCGGTTTCATCCAAGCTTTCCAATCTCACTAGGATTTCCTGCCGAAGGCACGTTGGGGATAACAACACGCACACGGGCAAAACCCTTATCCTGCGGGc             <  6:282366/148‑1 (MQ=255)                                                                                                                                ccccATTCGAACCGGGCCTGGCGTTTCATGATCAGGTCA‑AATCGTTCCTGCGGTTTCATCCAAGCTTTCCAATCTCACTATGATTTCCTGCCGAAGGCACGTTGGTGATAACAACACGCACACCGGCAAAACCCTTATCCTGCGGGTAg           <  2:235814/149‑1 (MQ=255)                                                                                                                                ccccATTCGAACCGGGCCTGGCGTTTCATGATCAGGTCA‑AATCGTTCCTGCGGTTTCATCCAAGCTTTCCAATCTCACTAGGATTTCCTGCCGAAGGCACGTTGGGGATAACAACACGCACACCGGCAAAACCCTTATCCTGCGGGCAg           <  7:331875/149‑1 (MQ=255)                                                                                                                                 cccATTCGAACCGGGCCTGGCGTTTCATGATCAGGTCA‑AATCGTTCCTGCGGTTTCATCCAAGCTTTCCAATCTCACTAGGATTTCCTGCCGAAGGCACGTTGGGGATAACAACACGCACACCGGCAAAACCCTTATCCTGCGGGCAgg          >  6:352191/1‑149 (MQ=255)                                                                                                                                  ccATTCGAACCGGGCCTGGCGTTTCATGATCAGGTCA‑AATCGTTCCTGCGGTTTCATCCAAGCTTTCCAATCTCACTAGGATTTCCTGCCGAAGGCACGTTGGGGATAACAACACGCACACCGGCAAAACCCTTATCCTGCGGGCAgg          >  6:350511/1‑148 (MQ=255)                                                                                                                                         aaCCGGGCCTGGCGTTTCATGATCAGGTCA‑AATCGTTCCTGCGGTTTCATCCAAGCTTTCCAATCTCACTAGGATTTCCTGCCGAAGGCACGTTGGGGATAACAACACGCACACCGGCAAAACCCTTATCCTGCGGGCAGGTAACcgcg  >  8:72332/1‑149 (MQ=255)                                                                                                                                            |                                                                                                                                                   AAACACCTTCTCTGGGTTGGAAAGGAGATGAATTGTGCGCCCCAGCTTTCGACTATGCAGTCTGCATATGCGAGAGCCTTTAGGTGCCTCTCGTGGAACCGCCAGCGTGGACGGTACATATGCCTGGCCCCATTCGAACCGGGCCTGGCGTTTCATGATCAGGTCA‑AATCGTTCCTGCGGTTTCATCCAAGCTTTCCAATCTCACTAGGATTTCCTGCCGAAGGCACGTTGGGGATAACAACACGCACACCGGCAAAACCCTTATCCTGCGGGCAGGTAACCGCG  >  NC_002947/6166056‑6166340

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑