Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	495,966	A→T	6.2%	L23Q (CTG→CAG)	PP_0408 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	495,966	0	A	T	6.2%	244.6 / 5.0	81	L23Q (CTG→CAG)	PP_0408	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (30/46);  new base T (2/3);  total (32/49)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.94e-01

CGGCCTGCAGGGTCTGCTGCTCAGCCTTGGGCAGCAGCCGTTCCAGCGCCAGTGCATCCTCCTGGCTGCGTTCCAGCAAGGGTGGGCGTGGGGCCTCGGCAGCCGGTGCCATGGCATTTGCAGGTGGTTTTTCGGCATCGGCGGCCAG‑GGC‑GCCGAGTGGAAGGACCGAGGCCAGGCAGCACATTGCCAG‑CGTCGTGCGATAAAGTGTGGACATGAGTCAACCCAAGGCCAGAATGAAACCGGCAGCCTAATAGTATTTGCCCGTGACGGCCATGCTGCATGGCCGTCTT  >  NC_002947/495820‑496109                                                                                                                                                   |                                                                                                                                                   cGGCCTGCAGGGTCTGCTGCTCAGCCTTGGGCAGCAGCCGTTCCAGCGCCAGTGCATCCTCCTGGCTGCGTTCCAGCAAGGGTGGGCGTGGGGCCTCGGCAGCCGGTGCCATGGCATTTGCAGGTGGTTTTTCGGCATCGGCGGCCAg‑g                                                                                                                                                 <  3:252903/149‑1 (MQ=255) cGGCCTGCAGGGTCTGCTGCTCAGCCTTGGGCAGCAGCCGTTCCAGCGCCAGTGCATCCTCCTGGCTGCGTTCCAGCAAGGGTGGGCGTGGGGCCTCGGCAGCCGGTGCCATGGCATTTGCAGGTGGTTTTTCGGCATCGGCGGCCAg‑g                                                                                                                                                 >  7:118842/1‑149 (MQ=255)  ggCCTGCAGGGTCTGCTGCTCAGCCTTGGGCAGCAGCCGTTCCAGCGCCAGTGCATCCTCCTGGCTGCGTTCCAGCAAGGGTGGGCGTGGGGCCTCGGCAGCCGGTGCCATGGCATTTGCAGGTGGTTTTTCGGCATCGGCGGCCAg‑gg                                                                                                                                                <  5:107120/149‑1 (MQ=255)     cTGCAGGGTCTGCTGCTCAGCCTTGGGCAGCAGCCGTTCCAGCGCCAGTGCATCCTCCTGGCTGCGTTCCAGCAAGGGTGGGCGTGGGGCCTCGGCAGCCGGTGCCATGGCATTTGCAGGTGGTTTTTCGGCATCGGCGCCCTG‑Gcc‑gc                                                                                                                                            >  5:98906/1‑149 (MQ=255)      tGCAGGGTCTGCTGCTCAGCCTTGGGCAGCAGCCGTTCCAGCGCCAGTGCATCCTCCTGGCTGCGTTCCAGCAAGGGTGGGCGTGGGGCCTCGGCAGCCGGTGCCATGGCATTTGCAGGTGGTTTTTCGGCATCGGCGGCCAG‑GGC‑Gcc                                                                                                                                           >  5:28389/1‑149 (MQ=255)      tGCAGGGTCTGCTGCTCAGCCTTGGGCAGCAGCCGTTCCAGCGCCAGTGCATCCTCCTGGCTGCGTTCCAGCAAGGGTGGGCGTGGGGCCTCGGCAGCCGGTGCCATGGCATTTGCAGGTGGTTTTTCGGCATCGGCGGCCAG‑GGC‑Gcc                                                                                                                                           >  3:133988/1‑149 (MQ=255)        cAGGGTCTGCTGCTCAGCCTTGGGCAGCAGCCGTTCCAGCGCCAGTGCATCCTCCTGGCTGCGTTCCAGCAAGGGTGGGCGTGGGGCCTCGGCAGCCGGTGCCATGGCATTTGCAGGTGGTTTTTCGGCATCGGCGGCCAG‑GGC‑Gcc                                                                                                                                           <  8:118842/147‑1 (MQ=255)         aGGGTCTGCTGCTCAGCCTTGGGCAGCAGCCGTTCCAGCGCCAGTGCATCCTCCTGGCTGCGTTCCAGCAAGGGTGGGCGTGGGGCCTCGGCAGCCGGTGCCATGGCATTTGCAGGTGGTTTTTCGGCATCGGCGGCCAG‑GGC‑GCCGAg                                                                                                                                        <  5:253584/149‑1 (MQ=255)         aGGGTCTGCTGCTCAGCCTTGGGCAGCAGCCGTTCCAGCGCCAGTGCATCCTCCTGGCTGCGTTCCAGCAAGGGTGGGCGTGGGGCCTCGGCAGCCGGTGCCATGGCATTTGCAGGTGGTTTTTCGGCATCGGCGGCCAG‑GGC‑GCCGAg                                                                                                                                        >  3:179023/1‑149 (MQ=255)          gggTCTGCTGCTCAGCCTTGGGCAGCAGCCGTTCCAGCGCCAGTGCATCCTCCTGGCTGCGTTCCAGCAAGGGTGGGCGTGGGGCCTCGGCAGCCGGTGCCATGGCATTTGCAGGTGGTTTTTCGGCATCGGCGGCCAG‑GGC‑GCCGAGt                                                                                                                                       <  5:225688/149‑1 (MQ=255)          gggTCTGCTGCTCAGCCTTGGGCAGCAGCCGTTCCAGCGCCAGTGCATCCTCCTGGCTGCGTTCCAGCAAGGGTGGGCGTGGGGCCTCGGCAGCCGGTGCCATGGCATTTGCAGGTGGTTTTTCGGCATCGGCGGCCAG‑GGC‑GCCGAGt                                                                                                                                       <  3:156991/149‑1 (MQ=255)           ggTCTGCTGCTCAGCCTTGGGCAGCAGCCGTTCCAGCGCCAGTGCATCCTCCTGGCTGCGTTCCAGCAAGGGTGGGCGTGGGGCCTCGGCAGCCGGTGCCATGGCATTTGCAGGTGGTTTTTCGGCATCGGCGGCCAG‑GGC‑GCCGAGTg                                                                                                                                      <  2:186836/149‑1 (MQ=255)           ggTCTGCTGCTCAGCCTTGGGCAGCAGCCGTTCCAGCGCCAGTGCATCCTCCTGGCTGCGTTCCAGCAAGGGTGGGCGTGGGGCCTCGGCAGCCGGTGCCATGGCATTTGCAGGTGGTTTTTCGGCATCGGCGCCCTG‑GCC‑GCCGatg                                                                                                                                       <  8:31283/148‑3 (MQ=255)            gTCTGCTGCTCAGCCTTGGGCAGCAGCCGTTCCAGCGCCAGTGCATCCTCCTGGCTGCGTTCCAGCAAGGGTGGGCGTGGGGCCTCGGCAGCCGGTGCCATGGCATTTGCAGGTGGTTTTTCGGCATCGGCGCCCTG‑GCC‑GCCGatgcc                                                                                                                                     <  6:98906/149‑5 (MQ=255)             tCTGCTGCTCAGCCTTGGGCAGCAGCCGTTCCAGCGCCAGTGCATCCTCCTGGCTGCGTTCCAGCAAGGGTGGGCGTGGGGCCTCGGCAGCCGGTGCCATGGCATTTGCAGGTGGTTTTTCGGCATCGGCGGCCAG‑GGC‑GCCGAGTgg                                                                                                                                     >  5:53296/1‑148 (MQ=255)                 ctgctCAGCCTTGGGCAGCAGCCGTTCCAGCGCCAGTGCATCCTCCTGGCTGCGTTCCAGCAAGGGTGGGCGTGGGGCCTCGGCAGCCGGTGCCATGGCATTTGCAGGTGGTTTTTCGGCATCGGCGGCCAG‑GGC‑GCCGAGTGGaa                                                                                                                                   <  3:352264/146‑1 (MQ=255)                 ctgctCAGCCTTGGGCAGCAGCCGTTCCAGCGCCAGTGCATCCTCCTGGCTGCGTTCCAGCAAGGGTGGGCGTGGGGCCTCGGCAGCCGGTGCCATGGCATTTGCAGGTGGTTTTTCGGCATCGGCGGCCAG‑GGC‑GCCGAGTGGaa                                                                                                                                   <  1:1015/146‑1 (MQ=255)                 ctgctCAGCCTTGGGCAGCAGCCGTTCCAGCGCCAGTGCATCCTCCTGGCTGCGTTCCAGCAAGGGTGGGCGTGGGGCCTCGGCAGCCGGTGCCATGGCATTTGCAGGTGGTTTTTCGGCATCGGCGGCCAG‑GGC‑GCCGAGTGGaa                                                                                                                                   <  6:248522/146‑1 (MQ=255)                       aGCCTTGGGCAGCAGCCGTTCCCGCGCCAGTGCATCCTCCTGGCTGCGTTCCAGCAAGGGTGGGCGTGGGGCCTCGGCAGCCGGTGCCATGGCATTTGCAGGTGGTTTTTCGGCATCGGCGGCCAG‑GGC‑GCCGAGTTGAAGGACCGa                                                                                                                            <  1:326905/147‑1 (MQ=255)                       aGCCTTGGGCAGCAGCCGTTCCAGCGCCAGTGCATCCTCCTGGCTGCGTTCCAGCAAGGGTGGGCGTGGGGCCTCGGCAGCCGGTGCCATGGCATTTGCAGGTGGTTTTTCGGCATCGGCGGCCAG‑GGC‑GCCGAGTGGAAGGACCGa                                                                                                                            <  1:332125/147‑1 (MQ=255)                       aGCCTTGGGCAGCAGCCGTTCCAGCGCCAGTGCATCCTCCTGGCTGCGTTCCAGCAAGGGTGGGCGTGGGGCCTCGGCAGCCGGTGCCATGGCATTTGCAGGTGGTTTTTCGGCATCGGCGGCCAG‑GGC‑GCCGAGTGGAAGGACCGAgg                                                                                                                          >  5:139076/1‑149 (MQ=255)                       aGCCTTGGGCAGCAGCCGTTCCAGCGCCAGTGCATCCTCCTGGCTGCGTTCCAGCAAGGGTGGGCGTGGGGCCTCGGCAGCCGGTGCCATGGCATTTGCAGGTGGTTTTTCGGCATCGGCGGCCAG‑GGC‑GCCGAGTGGAAGGACCGAg                                                                                                                           <  6:205843/148‑1 (MQ=255)                       aGCCTTGGGCAGCAGCCGTTCCAGCGCCAGTGCATCCTCCTGGCTGCGTTCCAGCAAGGGTGGGCGTGGGGCCTCGGCAGCCGGTGCCATGGCATTTGCAGGTGGTTTTTCGGCATCGGCGGCCAG‑GGC‑GCCGAGTGGAAGGACCGAg                                                                                                                           <  5:64293/148‑1 (MQ=255)                        gCCTTGGGCAGCAGCCGTTCCAGCGCCAGTGCATCCTCCTGGCTGCGTTCCAGCAAGGGTGGGCGTGGGGCCTCGGCAGCCGGTGCCATGGCATTTGCAGGTGGTTTTTCGGCATCGGCGGCCAG‑GGC‑GCCGAGTGGAAGGACCGAGGc                                                                                                                         <  8:128244/149‑1 (MQ=255)                        gCCTTGGGCAGCAGCCGTTCCAGCGCCAGTGCATCCTCCTGGCTGCGTTCCAGCAAGGGTGGGCGTGGGGCCTCGGCAGCCGGTGCCATGGCATTTGCAGGTGGTTTTTCGGCATCGGCGGCCAG‑GGC‑GCCGAGTGGAAGGACCGAGGc                                                                                                                         <  6:28389/149‑1 (MQ=255)                             gggCAGCAGCCGTTCCAGCGCCAGTGCATCCTCCTGGCTGCGTTCCAGCAAGGGTGGGCGTGGGGCCTCGGCAGCCGGTGCCATGGCATTTGCAGGTGGTTTTTCGGCATCGGCGGCCAG‑GGC‑GCCGAGTGGAAGGACCGAg                                                                                                                           <  2:282466/142‑1 (MQ=255)                             gggCAGCAGCCGTTCCAGCGCCAGTGCATCCTCCTGGCTGCGTTCCAGCAAGGGTGGGCGTGGGGCCTCGGCAGCCGGTGCCATGGCATTTGCAGGTGGTTTTTCGGCATCGGCGGCCAG‑GGC‑GCCGAGTGGAAGGACCGAg                                                                                                                           >  1:282466/1‑142 (MQ=255)                             gggCAGCAGCCGTTCCAGCGCCAGTGCATCCTCCTGGCTGCGTTCCAGCAAGGGTGGGCGTGGGGCCTCGGCAGCCGGTGCCATGGCATTTGCAGGTGGTTTTTCGGCATCGGCGGCCAG‑GGC‑GCCGAGTGGAAGGACCGAGGCCAgg                                                                                                                     <  5:257656/148‑1 (MQ=255)                              ggCAGCAGCCGTTCCAGCGCCAGTGCATCCTCCTGGCTGCGTTCCAGCAAGGGTGGGCGTGGGGCCTCGGCAGCCGGTGCCATGGCATTTGCAGGTGGTTTTTCGGCATCGGCGGCCAG‑GGC‑GCCGAGTGGAAGGACCGAGGCCAGgc                                                                                                                    <  4:133988/148‑1 (MQ=255)                                         ttCCAGCGCCAGTGCATCCTCCTGGCTGCGTTCCAGCAAGGGTGGGCGTGGGGCCTCGGCAGCCGGTGCCATGGCATTTGCAGGTGGTTTTTCGGCATCGGCGGCCAG‑GGC‑GCCGAGTGGAAGGACCGAGGCCAGGCAGCACATTGc                                                                                                          >  6:127933/1‑147 (MQ=255)                                         ttCCAGCGCCAGTGCATCCTCCTGGCTGCGTTCCAGCAAGGGTGGGCGTGGGGCCTCGGCAGCCGGTGCCATGGCATTTGCAGGTGGTTTTTCGGCATCGGCGGCCAG‑GGC‑GCCGAGTGGAAGGACCGAGGCCAGGCAGCACATTGc                                                                                                          >  6:122985/1‑147 (MQ=255)                                            cAGCGCCAGTGCATCCTCCTGGCTGCGTTCCAGCAAGGGTGGGCGTGGGGCCTCGGCAGCCGGTGCCATGGCATTTGCAGGTGGTTTTTCGGCATCGGCGGCCAG‑GGC‑GCCGAGTGGAAGGACCGAGGCCAGGCAGCACAtt                                                                                                            >  6:33465/1‑142 (MQ=255)                                            cAGCGCCAGTGCATCCTCCTGGCTGCGTTCCAGCAAGGGTGGGCGTGGGGCCTCGGCAGCCGGTGCCATGGCATTTGCAGGTGGTTTTTCGGCATCGGCGGCCAG‑GGC‑GCCGAGTGGAAGGACCGAGGCCAGGCAGCACAtt                                                                                                            <  5:33465/142‑1 (MQ=255)                                              gcgcCAGTGCATCCTCCTGGCTGCGTTCCAGCAAGGGTGGGCGTGGGGCCTCGGCAGCCGGTGCCATGGCATTTGCAGGTGGTTTTTCGGCATCGGCGGCCAG‑GGC‑GCCGAGTGGAAGGACCGAGGCCAGGCAGCACATTGCCAG‑cgtc                                                                                                  <  4:100498/149‑1 (MQ=255)                                                gcCAGTGCATCCTCCTGGCTGCGTTCCAGCAAGGGTGGGCGTGGGGCCTCGGCAGCCGGTGCCATGGCATTTGCTGGTGGTTTTTCGGCATCGGCGGCCAG‑GGC‑GCCGAGTGGAAGGCCCGAGGCCAGCCAGCACATTGCCCG‑cgtcgt                                                                                                >  4:230684/1‑149 (MQ=255)                                                    gTGCATCCTCCTGGCTGCGTTCCAGCAAGGGTGGGCGTGGGGCCTCGGCAGCCGGTGCCATGGCATTTGCAGGTGGTTTTTCGGCATCGGCGGCCAG‑GGC‑GCCGAGTGGAAGGACCGAGGCCAGGCAGCACATTGCCAG‑CGTCGTGCGa                                                                                            <  5:342381/149‑1 (MQ=255)                                                     tGCATCCTCCTGGCTGCGTTCCAGCAAGGGTGGGCGTGGGGCCTCGGCAGCCGGTGCCATGGCATTTGCAGGTGGTTTTTCGGCATCGGCGGCCAGTCGC‑GCCGAGTGGAAGGACCGAGGCCAGGCAGCACATTGCCAG‑CGTCGTGCGa                                                                                            <  1:271240/149‑1 (MQ=255)                                                      gCATCCTCCTGGCTGCGTTCCAGCAAGGGTGGGCGTGGGGCCTCGGCAGCCGGTGCCATGGAATTTGCAGGTGGTTTTTCGGCATCGGCGGCCAG‑GGC‑GGCGAGTGGAAGGACCGAGGCCAGGCAGCACATTGACAG‑CGTGGTGCGAt                                                                                           >  7:54050/1‑148 (MQ=255)                                                       cATCCTCCTGGCTGCGTTCCAGCAAGGGTGGGCGTGGGGCCTCGGCAGCCGGTGCCATGGCATTTGCAGGTGGTTTTTCGGCATCGGCGGCCAG‑GGC‑GCCGAGTGGAAGGACCGAGGCCAGGCAGCACATTGCCAG‑CGTCGTGCGATa                                                                                          >  3:353323/1‑148 (MQ=255)                                                         tcctccTGGCTGCGTTCCAGCAAGGGTGGGCGTGGGGCCTCGGCAGCCGGTGCCATGGCATTTGCAGGTGGTTTTTCGGCATCGGCGGCCAG‑GGC‑GCCGAGTGGAAGGACCGAGGCCAGGCAGCACATTGCCAG‑CGTCGTGCGATaaa                                                                                        <  3:257490/148‑1 (MQ=255)                                                         tcctccTGGCTGCGTTCCAGCAAGGGTGGGCGTGGGGCCTCGGCAGCCGGTGCCATGGCATTTGCAGGTGGTTTTTCGGCATCGGCGGCCAG‑GGC‑GCCGAGTGGAAGGACCGAGGCCAGGCAGCACATTGCCAG‑CGTCGTGCGATa                                                                                          >  4:257490/1‑146 (MQ=255)                                                           ctcctGGCTGCGTTCCAGCAAGGGTGGGCGTGGGGCCTCGGCAGCCGGTGCCATGGCATTTGCAGGTGGTTTTTCGGCATCGGCGGCCAG‑GGC‑GCCGAGTGGAAGGACCGAGGCCAGGCAGCACATTGCCAG‑CGTCGTGCGATAAAgtg                                                                                     <  6:139076/149‑1 (MQ=255)                                                              ctGGCTGCGTTCCAGCAAGGGTGGGCGTGGGGCCTCGGCAGCCGGTGCCATGGCATTTGCAGGTGGTTTTTCGGCATCGGCGGCCAG‑GGC‑GCCGAGTGGAAGGACCGAGGCCAGGCAGCACATTGCCAG‑CGTCGTGCGATAAAGtgtg                                                                                   <  3:46471/148‑1 (MQ=255)                                                              ctGGCTGCGTTCCAGCAAGGGTGGGCGTGGGGCCTCGGCAGCCGGTGCCATGGCATTTGCAGGTGGTTTTTCGGCATCGGCGGCCAG‑GGC‑GCCGAGTGGAAGGACCGAGGCCAGGCAGCACATTGCCAG‑CGTCGTGCGATAAAGtgtg                                                                                   <  7:335037/148‑1 (MQ=255)                                                                 gCTGCGTTCCAGCAAGGGTGGGCGTGGGGCCTCGGCAGCCGGTGCCATGGCATTTGCAGGTGGTTTTTCGGCATCGGCGGCCAG‑GGC‑GCCGAGTGGAAGGACCGAGGCCAGGCAGCACATTGCCAG‑CGTCGTGCGATAAAgt                                                                                      <  6:371878/142‑1 (MQ=255)                                                                 gCTGCGTTCCAGCAAGGGTGGGCGTGGGGCCTCGGCAGCCGGTGCCATGGCATTTGCAGGTGGTTTTTCGGCATCGGCGGCCAG‑GGC‑GCCGAGTGGAAGGACCGAGGCCAGGCAGCACATTGCCAG‑CGTCGTGCGATAAAgt                                                                                      >  5:371878/1‑142 (MQ=255)                                                                    gCGTTCCAGCAAGGGTGGGCGTGGGGCCTCGGCAGCCGGTGCCATGGCATTTGCAGGTGGTTTTTCGGCATCGGCGGCCAG‑GGC‑GCCGAGTGGAAGGACCGAGGCCAGGCAGCACATTGCCAG‑CGTCGTGCGATAAAGTGTGGACATGa                                                                            <  2:320485/149‑1 (MQ=255)                                                                          cAGCAAGGGTGGGCGTGGGGCCTCGGCAGCCGGTGCCATGGCATTTGCAGGTGGTTTTTCGGCATCGGCGGCCAG‑GGC‑GCCGAGTGGAAGGACCGAg                                                                                                                           <  4:182076/97‑1 (MQ=255)                                                                          cAGCAAGGGTGGGCGTGGGGCCTCGGCAGCCGGTGCCATGGCATTTGCAGGTGGTTTTTCGGCATCGGCGGCCAG‑GGC‑GCCGAGTGGAAGGACCGAg                                                                                                                           >  3:182076/1‑97 (MQ=255)                                                                           aGCAAGGGTGGGCGTGGGGCCTCGGCAGCCGGTGCCATGGCATTTGCAGGTGGTTTTTCGGCATCGGCGGCCAG‑GGC‑GCCGAGTGGAAGGACCGAGGCCAGGCAGCACATTGCCAG‑CGTCGTGCGATAAAGTGTGGACATGAGTCAAcc                                                                     >  8:48534/1‑149 (MQ=255)                                                                            gCAAGGGTGGGCGTGGGGCCTCGGCAGCCGGTGCCATGGCATTTGCAGGTGGTTTTTCGGCATCGGCGGCCAG‑GGC‑GCCGAGTGGAAGGACCGAGGCCAGGCAGCACATTGCCAG‑CGTCGTGCGATAAAGTGTGGACATGAGTCAAccc                                                                    <  4:179023/149‑1 (MQ=255)                                                                                  gTGGGCGTGGGGCCTCGGCAGCCGGTGCCATGGCATTTGCAGGTGGTTTTTCGGCATCGGCGGCCAG‑GGC‑GCCGAGTGGAAGGACCGAGGCCAGGCAGCACATTGCCAG‑CGTCGTGCGATAAAGTGTGGACATGAGTCAACCCAAGGc                                                               >  4:339982/1‑148 (MQ=255)                                                                                   tGGGCGTGGGGCCTCGGCAGCCGGTGCCATGGCATTTGCAGGTGGTTTTTCTGCATCGGCGGCCAT‑GGC‑GCCGAGTGGAAGGACCGAGGCCAGGCAGCACATTGCCAG‑CGTCGTGCGATAAAGTGTGTACATGAGTCAACCCAAGGCCa                                                             <  3:230684/149‑1 (MQ=255)                                                                                   tGGGCGTGGGGCCTCGGCAGCCGGTGCCATGGCATTTGCAGGTGGTTTTTCGGCATCGGCGGCCAG‑GGC‑GCCGAGTGGAAGGACCGAGGCCAGGCAGCACATTGCCAG‑CGTCGTGCGATAAAGTGTGGACATGAGTCAACCCAAGGCCa                                                             >  7:84657/1‑149 (MQ=255)                                                                                      gCGTGGGGCCTCGGCAGCCGGTGCCATGGCATTTGCAGGTGGTTTTTCGGCATCGGCGGCCAG‑GGC‑GCCGAGTGGAAGGACCGAGGCCAGGCAGCACATTGCCAG‑CGTCGTGCGATAAAGTGTGGACATGAGTCAACCCAAGGCCAGaa                                                          <  4:353323/149‑1 (MQ=255)                                                                                              ccTCGGCAGCCGGTGCCATGGCATTTGCAGGTGGTTTTTCGGCATCGGCGGCCAG‑GGC‑GCCGAGTGGAAGGACCGAGGCCAGGCAGCACATTGCCAG‑CGTCGTGCGATAAAGTGTGGACATGAGTCAACCCAAGGCCAGAATGAAACCg                                                  >  6:274440/1‑149 (MQ=255)                                                                                                  ggCAGCCGGTGCCATGGCATTTGCAGGTGGTTTTTCGGCATCGGCGGCCAG‑GGC‑GCCGAGTGGAAGGACCGAGGCCAGGCAGCACATTGCCAG‑CGTCGTGCGATAAAGTGTGGACATGAGTCAACCCAAGGCCAGAATGAAACCGGCa                                               <  6:53296/148‑1 (MQ=255)                                                                                                    cAGCCGGTGCCATGGCATTTGCAGGTGGTTTTTCGGCATCGGCGGCCAG‑GGC‑GCCGAGTGGAAGGACCGAGGCCAGGCAGCACATTGCCAG‑CGTCGTGCGATAAAGTGTGGACATGa                                                                            <  5:19661/117‑1 (MQ=255)                                                                                                    cAGCCGGTGCCATGGCATTTGCAGGTGGTTTTTCGGCATCGGCGGCCAG‑GGC‑GCCGAGTGGAAGGACCGAGGCCAGGCAGCACATTGCCAG‑CGTCGTGCGATAAAGTGTGGACATGa                                                                            >  6:19661/1‑117 (MQ=255)                                                                                                       ccGGTGCCATGGCATTTGCAGGTGGTTTTTCGGCATCGGCGGCCAG‑GGC‑GCCGAGTGGAAGGACCGAGGCCAGGCAGCACATTGCCAG‑CGTCGTGCGATAAAGTGTGGACATGAGTCAACCCAAGGCCAGAATGAAACCGGCAGCCTaa                                         <  3:339982/149‑1 (MQ=255)                                                                                                         ggTGCCATGGCATTTGCAGGTGGTTTTTCGGCATCGGCGGCCAG‑GGC‑GCCGAGTGGAAGGACCGAGGCCAGGCAGCACATTGCCAG‑CGTCGTGCGATAAAGTGTGGACATGAGTCAACCCAAGGCCAGAATGAAACCGGCAGCCTAATa                                       <  5:127933/149‑1 (MQ=255)                                                                                                         ggTGCCATGGCATTTGCAGGTGGTTTTTCGGCATCGGCGGCCAG‑GGC‑GCCGAGTGGAAGGACCGAGGCCAGGCAGCACATTGCCAG‑CGTCGTGCGATAAAGTGTGGACATGAGTCAACCCAAGGCCAGAATGAAACCGGCAGCCTAATa                                       <  8:36987/149‑1 (MQ=255)                                                                                                          gTGCCATGGCATTTGCAGGTGGTTTTTCGGCATCGGCGGCCAG‑GGC‑GCCGAGTGGAAGGACCGAGGCCAGGCAGCACATTGCCAG‑CGTTGTGCGATAAAGTGTGGACATGAGTCAACCCAAGGCCAGAATGAAACCGGCAGCCTAATAg                                      <  1:103840/149‑1 (MQ=255)                                                                                                             ccATGGCATTTGCAGGTGGTTTTTCGGCGTCGGCGGCCAG‑GGCGGCCGAGTGGAAGGACCGAGGCCAGGCAGCACATTGCCAG‑CGTCGTGCGATAAAGTGTGGACATGAGTCAACCCAAGGCCAGAATGAAACCGGCAGCCTAATAGTa                                    >  1:251669/1‑149 (MQ=255)                                                                                                               aTGGCATTTGCAGGTGGTTTTTCGGCATCGGCGGCCAG‑GGC‑GCCGAGTGGAAGGACCGAGGCCAGGCAGCACATTGCCAG‑CGTCGTGCGATAAAGTGTGGACATGAGTCAACCCAAGGCCAGAATGAAACAGGCAGCCTAATAGTAttt                                 >  6:68215/1‑149 (MQ=255)                                                                                                                  ccATTTGCAGGTGTTTTATCGGCATCGGCGGCCAG‑GGC‑GCCGAGTGTAAGGACCGAGGCCAGGCAGCACATTGCCAG‑CGTCGTGCGATAAAGTGTGGACATGAGTCAACCCAAGGCCAGAATGAAACCGGCAGCCTAATAGTATTTGc                               <  8:54050/147‑1 (MQ=255)                                                                                                                   cATTTGCAGGTGGTTTTTCGGCATCGGCGGCCAG‑GGC‑GCCGAGTGGAAGGACCGAGGCCAGGCAGCACATTGCCAG‑CGTCGTGCGATAAAGTGTGGACATGAGTCAACCCAAGGCCAGAATGAAACCGGCAGCCTAATAGTATTTGccc                             <  4:213742/149‑1 (MQ=255)                                                                                                                    aTTTGCAGGTGGTTTTTCGGCATCGGCGGCCAG‑GGC‑GCCGAGTGGAAGGACCGAGGCCAGGCAGCACATTGCCAG‑CGTCGTGCGATAAAGTGTGGACATGAGTCAACCCAAGGCCAGAATGAAACCGGCAGCCTAATAGTATTTGCCCg                            >  1:334934/1‑149 (MQ=255)                                                                                                                         cAGGTGGTTTTTCGGCATCGGCGGCCAG‑GGC‑GCCGAGTGGAATGACCGAGGCCAGGCAGCACATTGCCAG‑CGTCGTGCGATAAAGTGTGGACATGAGTCAACCCAAGGCCAGAATGAAACCGGCAGCCTAATAGTATTTGCCCGTGAc                        <  6:341655/148‑1 (MQ=255)                                                                                                                         cAGGTGGTTTTTCGGCATCGGCGGCCAG‑GGC‑GCCGAGTGGAAGGACCGAGGCCAGGCAGCACATTGCCAG‑CGTCGTGCGATAAAGTGTGGACATGAGTCAACCCAAGGCCAGAATGAAACCGGCAGCCTAATAGTATTTGCCCGTGACg                       <  2:111641/149‑1 (MQ=255)                                                                                                                           ggtggtTTTTCGGCATCGGCGGCCAG‑GGC‑GCCGAGTGGAAGGACCGAGGCCAGGCAGCACATTGCCAG‑CGTCGTGCGATAAAGTGTGGACATGATTCAACCCAAGGCCAGAATGAAACCGGCAGCCTAATAGTATTTGCCCGTGACGGc                     <  8:84657/149‑1 (MQ=255)                                                                                                                             tggtTTTTCGGCATCGGCGGCCAG‑GGC‑GCCGAGTGGAAGGACCGAGGCCAGGCAGCACATTGCCAGTCGTCGTGCGATAAAGTGTGGACATGAGTCAACCCAAGGCCAGAATGAAACCGGCAGCCTAATAGTATTTGCCCGTGACGGc                     >  1:289537/1‑148 (MQ=255)                                                                                                                              ggtTTTTCGGCATCGGCGGCCAG‑GGC‑GCCGAGTGGAAGGACCGAGGCCAGGCAGCACATTGCCAG‑CGTCGTGCGATAAAGTGTGGACATGAGTCAACCCAAGGCCAGAATGAAACCGGCAGCCTAATAGTATTTGCCCGTGACGGCCAt                  >  3:100913/1‑149 (MQ=255)                                                                                                                              ggtTTTTCGGCATCGGCGGCCAG‑GGC‑GCCGAGTGGAAGGACCGAGGCCAGGCAGCACATTGCCAG‑CGTCGTGCGATAAAGTGTGGACATGAGTCAACCCAAGGCCAGAATGAAACCGGCAGCCTAATAGTATTTGCCCGTGACGGCCAt                  >  1:185883/1‑149 (MQ=255)                                                                                                                                      ccactCGGCGCCCTG‑GCC‑GCCGA‑TGGAAGGACCGAGGCCAGGCAGCACATTGCCAG‑CGTCGTGCGATAAAGTGTGGACATGAGTCAAcc                                                                     >  4:31414/5‑89 (MQ=37)                                                                                                                                      ccactCGGCGCCCTG‑GCC‑GCCGA‑TGGAAGGACCGAGGCCAGGCAGCACATTGCCAG‑CGTCGTGCGATAAAGTGTGGACATGAGTCAAcc                                                                     <  3:31414/85‑1 (MQ=37)                                                                                                                                       gCATCGGCGGCCAG‑GGC‑GCCGAGTGGAAGGACCGAGGCCAGGCAGCACATTGCCAG‑CGTCGTGCGATAAAGTGTGGACATGAGTCAACCCAAGGCCAGAATGAAACCGGCAGCCTAATAGTATTTGCCCGTGACGGCCATGCTGCATgg         >  7:38947/1‑149 (MQ=255)                                                                                                                                         aTCGGCGGCCAG‑GGC‑GCCGAGTGGAAGGACCGAGGCCAGGCAGCACATTGCCAG‑CGTCGTGCGATAAAGTGTGGACATGAGTCAACCCAAGGCCAGAATGAAACCGGCAGCCTAATAGTATTTGCCCGTGACGGCCATGCTGCATGGcc       >  2:236028/1‑149 (MQ=255)                                                                                                                                         aTCGGCGGCCAG‑GGC‑GCCGAGTGGAAGGACCGAGGCCAGGCAGCACATTGCCAG‑CGTCGTGCGATAAAGTGTGGACATGAGTCAACCCAAGGCCAGAATGAAACCGGCAGCCTAATAGTATTTGCCCGTGACGGCCATGCTGCATGGcc       >  7:206047/1‑149 (MQ=255)                                                                                                                                            ggcggcCAG‑GGC‑GCCGAGTGGAAGGACCGAGGCCAGGCAGCACATTGCCAG‑CGTCGTGCGATAAAGTGTGGACATGAGTCAACCCAAGGCCAGAATGAAACCGGCAGCCTAATAGTATTTGCCCGTGACGGCCATGCAGCATGGCCGTc    <  7:48534/149‑1 (MQ=255)                                                                                                                                              cggcCAG‑GGC‑GCCGAGTGGAAGGACCGAGGCCAGGCAGCACATTGCCAG‑CGTCGTGCGATAAAGTGTGGACATGAGTCAACCCAAGGCCAGAATGAAACCGGCAGCCTAATAGTATTTGCCCGTGACGGCCATGCAGCATGGCCGTcac  <  2:334934/149‑3 (MQ=255)                                                                                                                                                   |                                                                                                                                                   CGGCCTGCAGGGTCTGCTGCTCAGCCTTGGGCAGCAGCCGTTCCAGCGCCAGTGCATCCTCCTGGCTGCGTTCCAGCAAGGGTGGGCGTGGGGCCTCGGCAGCCGGTGCCATGGCATTTGCAGGTGGTTTTTCGGCATCGGCGGCCAG‑GGC‑GCCGAGTGGAAGGACCGAGGCCAGGCAGCACATTGCCAG‑CGTCGTGCGATAAAGTGTGGACATGAGTCAACCCAAGGCCAGAATGAAACCGGCAGCCTAATAGTATTTGCCCGTGACGGCCATGCTGCATGGCCGTCTT  >  NC_002947/495820‑496109

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑