Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	503,831	A→T	15.5%	intergenic (+34/‑117)	PP_0414 → / → rpe	polyamine ABC transporter permease/ribulose‑5‑phosphate 3‑epimerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	503,831	0	A	T	15.5%	107.1 / 11.3	45	intergenic (+34/‑117)	PP_0414/rpe	polyamine ABC transporter permease/ribulose‑5‑phosphate 3‑epimerase
Reads supporting (aligned to +/- strand):  ref base A (23/15);  new base T (2/5);  total (25/20)
Fisher's exact test for biased strand distribution p-value = 2.14e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.47e-01

CCGACCATTGCCGCGGCGGCGACCTTGCTGATTGCGTTCTCGGTGTTGTTGCTGCTGACCCTGGAGTGGTTGCGTGGGCGTAGCGAGAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTG‑TACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTTCATCGCTTTCCCTGAGTTACAATGCGCGCCACCGTGAATTTGCCAACAGGTGCGCCATGCAGCCCTACGCTATTGCCCCCTC  >  NC_002947/503684‑503973                                                                                                                                                     |                                                                                                                                                ccGACCATTGCCGCGGCGGCGACCTTGCTGATTGCGTTCTCGGTGTTGTTGCTGCTGACCCTGGAGTGGTTGCGTGGGCGTAGCGAGAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTG‑TACCGGCCTCTTCGCGGCTaa                                                                                                                                                <  3:171425/149‑1 (MQ=255)  cGACCATTGCCGCGGCGGCGACCTTGCTGATTGCGTTCTCGGTGTTGTTGCTGCTGACCCTGGAGTGGTTGCGTGGGCGTAGCGAGAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTG‑TACCGGCCTCTTCGCGGCTa                                                                                                                                                 >  5:295167/1‑147 (MQ=255)            cgcgGCGGCGACCTTGCTGATTGCGTTCTCGGTGTTGTTGCTGCTGACCCTGGAGTGGTTGCGTGGGCGTAGCGAGAAGCTGAGGACTCAACAGTCGGCTTGATGATGGCTTGCCTG‑TACCGGCCTCTTCGCGGCTAAAGCCG‑GAagag                                                                                                                                    <  6:120423/149‑1 (MQ=255)            cgcgGCGGCGACCTTGCTGATTGCGTTCTCGGTGTTGTTGCTGCTGACCCTGGAGTGGTTGCGTGGGCGTAGCGAGAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTG‑TGCCGGCCTCTTC‑CGGCTTTAGCCGCGAagag                                                                                                                                    <  7:345938/149‑1 (MQ=39)            cgcgGCGGCGACCTTGCTGATTGCGTTCTCGGTGTTGTTGCTGCTGACCCTGGAGTGGTTGCGTGGGCGTAGCGAGAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTG‑TGCCGGCCTCTTC‑CGGCTTTAGCCGCGAagag                                                                                                                                    <  5:142168/149‑1 (MQ=39)              cggcggcgACCTTGCTGATTGCGTTCTCGGTGTTGTTGCTGCTGACCCTGGAGTGGTTGCGTGGGCGTAGCGAGAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTG‑TACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGc                                                                                                                                  >  5:337911/1‑149 (MQ=255)               ggcggcgACCTTGCTGATTGCGTTCTCGGTGTTGTTGCTGCTGACCCTGGAGTGGTTGCGTGGGCGTAGCGAGAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTG‑TGCCGGCCTCTTC‑CGGCTTTAGCCGCGAAGAGGcc                                                                                                                                 <  3:231223/149‑1 (MQ=38)                 cggcgACCTTGCTGATTGCGTTCTCGGTGTTGTTGCTGCTGACCCTGGAGTGGTTGCGTGGGCGTAGCGAGAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTG‑TACCAGCCTCTTC‑CGGCTTTAGCCGCGAAGAGGCCgg                                                                                                                               <  4:222413/149‑1 (MQ=39)                     gaagttGCTGATTGCGTTCTCGGTGTTGTTGCTTCTGACCCTGGAGTGGTTGCGTGGGGTTAGCGAGAAGCTGAGGACTCAACAGCCGGCGTGATGATGGCTTGCCTG‑TACCGGCCTCTTCGCGGCTAAAGCCG‑TAAGAGGCCGGcaca                                                                                                                           <  6:211093/145‑1 (MQ=255)                     gACCTTGCTGATTGCGTTCTCGGTGTTGTTGCTGCTGACCCTGGAGTGGTTGCGTGGGCGTAGCGAGAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTG‑TGCCGGCCTCTTC‑CGGCTTTAGCCGCGAAGAGGCCGGtaca                                                                                                                           <  7:3514/149‑1 (MQ=38)                       ccTTGCTGATTGCGTTCTCGGTGTTGTTGCTGCTGACCCTGGAGTGGTTGCGTGGGCGTAGCGAGAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTG‑TACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAgg                                                                                                                         <  7:207675/149‑1 (MQ=255)                            cTGATTGCGTTCTCGGTGTTGTTGCTGCTGACCCTGGAGTGGTTGCGTGGGCGTAGCGAGAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTG‑TACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCg                                                                                                                    >  4:224358/1‑149 (MQ=255)                               aTTGCGTTCTCGGTGTTGTTGCTGCTGACCCTGGAGTGGTTGCGTGGGCGTAGCGAGAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTG‑TACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGt                                                                                                                   <  3:224358/147‑1 (MQ=255)                                   cGTTCTCGGTGTTGTTGCTGCTGACCCTGGAGTGGTTGCGTGGGCGTAGCGAGAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTG‑TACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTc                                                                                                              <  4:310924/148‑1 (MQ=255)                                   cGTTCTCGGTGTTGTTGCTGCTGACCCTGGAGTGGTTGCGTGGGCGTAGCGAGAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTG‑TACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTc                                                                                                              >  3:183245/1‑148 (MQ=255)                                    gTTCTCGGTGTTGTTGCTGCTGACCCTGGAGTGGTTGCGTGGGCGTAGCGAGAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTG‑TACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGa                                                                                                            >  5:372183/1‑149 (MQ=255)                                       ctcGGTGTTGTTGCTGCTGACCCTGGAGTGGTTGCGTGGGCGTAGCGAGAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTG‑TACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGaca                                                                                                          <  6:337911/148‑1 (MQ=255)                                        tcGGTGTTGTTGCTGCTGACCCTGGAGTGGTTGCGTGGGCGTAGCGAGAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTG‑TACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGacac                                                                                                         >  7:93536/1‑148 (MQ=255)                                                         gACCCTGGAGTGGTTGCGTGGGCGTAGCGAGAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTG‑TACCGGCCTCTTCGCGCCTAAAGCCG‑GAAGAGGCCCGCACAGGTTTCGTTGGTCGACACCAATTGATAAAAATCAg                                                                                        >  4:235197/1‑148 (MQ=255)                                                          aCCCTGGAGTGGTTGCGTGGGCGTAGCGAGAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTG‑TACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGcc                                                                                      >  6:258961/1‑149 (MQ=255)                                                                gAGTGGTTGCGTGGGCGTAGCGAGAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTG‑TACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTTCATc                                                                                <  6:295167/149‑1 (MQ=255)                                                                        gCGTGGGAGTAGCGAGAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTG‑TACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTTCATCGCTTTcc                                                                         <  3:235197/148‑1 (MQ=255)                                                                         cGTGGGCGTAGCGAGAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTG‑TACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTTCATCGCTTTCCCt                                                                       <  4:183245/149‑1 (MQ=255)                                                                             ggCGTAGCGAGAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTGTTACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTTCATCGCTTTCCGTGAg                                                                    >  1:93918/1‑149 (MQ=255)                                                                                          gCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTG‑TACCGGCCTCTTCGCGGGTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCg                                                                                                                    <  1:29196/87‑1 (MQ=255)                                                                                          gCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTG‑TACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCg                                                                                                                    >  2:29196/1‑87 (MQ=255)                                                                                                gACTCAACAGCCGGCTTGATGATGGCTTGCCTG‑TACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTTCATCGCTTTCCCTGAGTTACAATGCGCGCCACCGt                                                 >  4:348465/1‑148 (MQ=255)                                                                                                   tCAACAGCCGGCTTGATGATGGCTTGCCTG‑TACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATGCCAATCAGCCTTCATAGCTTTCCCTGAGTTACAATGCGCGCCACCGTGaag                                             >  2:14109/1‑148 (MQ=255)                                                                                                   tCAACAGCCGGCTTGATGATGGCTTGCCTG‑TACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTTCATCGCTTTCCCTGAGTTACAATGCGCGCCACCGTGAAt                                             >  3:70372/1‑149 (MQ=255)                                                                                                   tCAACAGCCGGCTTGATGATGGCTTGCCTG‑TACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTTCATCGCTTTCCCTGAGTTACAATGCGCGCCACCCTGAAt                                             >  4:234588/1‑149 (MQ=255)                                                                                                     aaCAGCCGGCTTGATGATGGCTTGCCTG‑TACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACAAATCAGCCTTCATCGCTTTCCCTGAGTTACAATGAGCGCCACCGTGAAttt                                           >  8:196402/1‑149 (MQ=255)                                                                                                        aGCCGGCTTGATGATGGCTTGCCTG‑TACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTTCATCGCTTTCCCTGAGTTACAATGCGCGCCACCGTGAATTTGcc                                        <  6:140204/149‑1 (MQ=255)                                                                                                               ttGATGATGGCTTGCCTG‑TACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTTCATCGCTTTCCCTGAGTTACAATGCGCGCCACCGTGAATTTGCCAACAGGt                                 >  8:213836/1‑149 (MQ=255)                                                                                                                       acgcaaccTG‑TGCCGGCCTCTTC‑CGGCTTTAGCCGCGAAGAGGCCGGTACAGGTTGCGTTGGTAGACACCAATTGATACCAATCAGCCTTCATCGCTTTCCCTGAGTTACAATGCGCGCCACCGTGAATTTGCCAACAGGTGCGCCATg                         >  2:116981/7‑149 (MQ=25)                                                                                                                             ccTG‑TACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTTCATCGCTTGCCCTGAGTTACAATGCGCGCCACCGTGAATTTGCCAACAGGTGCGCCATGCAGcc                    <  5:258961/148‑1 (MQ=255)                                                                                                                               tG‑TACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTTCATCGCTTTCCCTGAGTTACAATGCGCGCCACCGTGAATTTGCCAACAGGTGCGcc                            >  8:165488/1‑138 (MQ=255)                                                                                                                               tG‑TACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTTCATCGCTTTCCCTGAGTTACAATGCGCGCCACCGTGAATTTGCCAACAGGTGCGcc                            <  7:165488/138‑1 (MQ=255)                                                                                                                               tG‑TACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTTCATCGCTTTCCCTGAGTTACAATGCGCGCCACCGTGAATTTGCCAACAGGTGCGCCATGCAGCCCTa                 >  8:25060/1‑149 (MQ=255)                                                                                                                                   aCCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTTCATCGCTTTCCCTGAGTTACAATGCGCGCCACCGTGAATTTGCCAACAGGTGCGCCATGCAGCCCTACg               <  7:25060/148‑1 (MQ=255)                                                                                                                                    ccGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTTCATCGCTGTCCCTGAGTTACAATGCGCGCCACCGTGAATTTGCCAACAGGTGCGCCATGCAGCCCTACGc              >  2:289990/1‑148 (MQ=255)                                                                                                                                         ctctTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTTCATCGCTTTCCCTGAGTTACAATGCGCGCCACCGTGAATTTGCCAACAGGTGCGCCATGCAGCCCTACGCTATTGc        >  5:37068/1‑149 (MQ=255)                                                                                                                                             ttccgGCTTTAGCCGCGAAGAGGCCGGTACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTTCATCGCTTTCCCTGAGTTACAATGCGCGCCACCGTGAATTTGCCAACAGGTGCGCCATGCAGCCCTACGCTATTGcccc     >  4:184667/4‑149 (MQ=255)                                                                                                                                             tCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTTCATCGCTTTCCCTGAGTTACAATGCGCGCCACCGTGAATTTGCCAACAGGTGCGCCATGCAGCCCTACGCTATTGccccc    >  1:248039/1‑149 (MQ=255)                                                                                                                                               gcgGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTTCATCGCTTTCCCTGAGTTACAATGCGCGCCACCGTGAAttt                                           <  2:97573/108‑1 (MQ=255)                                                                                                                                               gcgGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTTCATCGCTTTCCCTGAGTTACAATGCGCGCCACCGTGAATTTGCCAACAGGTGCGCCATGCAGCCCTACGCTATTGCCCCCTc  >  6:162083/1‑149 (MQ=255)                                                                                                                                               gcgGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTTCATCGCTTTCCCTGAGTTACAATGCGCGCCACCGTGAATTTGCCAACAGGTGCGCCATGCAGCCCTACGCTATTGCCCCCTc  >  1:18186/1‑149 (MQ=255)                                                                                                                                               gcgGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTTCATAGCTTTCCCTGAGTTACAATGCGCGCCACCGTGAAttt                                           >  1:97573/1‑108 (MQ=255)                                                                                                                                                     |                                                                                                                                                CCGACCATTGCCGCGGCGGCGACCTTGCTGATTGCGTTCTCGGTGTTGTTGCTGCTGACCCTGGAGTGGTTGCGTGGGCGTAGCGAGAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTG‑TACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTTCATCGCTTTCCCTGAGTTACAATGCGCGCCACCGTGAATTTGCCAACAGGTGCGCCATGCAGCCCTACGCTATTGCCCCCTC  >  NC_002947/503684‑503973

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑