Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	532,616	G→C	6.0%	noncoding (35/76 nt)	PP_t03 →	tRNA‑Thr

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	532,616	0	G	C	6.0%	248.7 / 3.5	78	noncoding (35/76 nt)	PP_t03	tRNA‑Thr
Reads supporting (aligned to +/- strand):  ref base G (31/42);  new base C (3/2);  total (34/44)
Fisher's exact test for biased strand distribution p-value = 6.48e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.83e-01

CCGCAAGCTCCGCGGGTATAGTTTAGTGGTAGAACCTCAGCCTTCCAAGCTGATGATGCGGGTTCGATTCCCGCTACCCGCTCCAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAAGGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACTATTTCTTCTGCGGAGGATTGTATCGATGGCTAAGGAAAAGTTTGATC  >  NC_002947/532468‑532757                                                                                                                                                     |                                                                                                                                              ccGCAAGCTCCGCGGGTATAGTTTAGTGGTAGAACCTCAGCCTTCCAAGCTGATGATGCGGGTTCGATTCCCGCTACCCGCTCCAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTgg                                                                                                                                               >  5:233287/1‑149 (MQ=255) ccGCAAGCTCCGCGGGTATAGTTTAGTGGTAGAACCTCAGCCTTCCAAGCTGATGATGCGGGTTCGATTCACGCTACACGCTCAAAGTTTGTAGTATTTTGCACAATGTGTTTAGCTATTGTAGATCAGTTGGTATAGCAAAACCTTgg                                                                                                                                               >  2:228096/1‑149 (MQ=255)  cGCAAGCTCCGCGGGTATAGTTTAGTGGTAGAACCTCAGCCTTCCAAGCTGATGATGCGGGTTCGATTCCCGCTACCCGCTCCAAGTTTGTCGGATTTTGCACAAAGTGTTTCGGTCTTGTAGCTCTGGTGGTAGAGCACACGCGTggg                                                                                                                                              >  8:242957/1‑148 (MQ=255)    cAAGCTCCGCGGGTATAGTTTAGTGGTAGAACCTCAGCCTTCCAAGCTGATGATGCGGGTTCGATTCCCGCTACCCGCTCCAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTaa                                                                                                                                            <  7:285480/149‑1 (MQ=255)      aGCTCCGCGGGTATAGTTTAGTGGTAGAACCTCAGCCTTCCAAGCTGATGATGCGGGTTCGATTCCCGCTACCCGCTCCAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTTGTAAgg                                                                                                                                          <  4:218664/149‑1 (MQ=255)      aGCTCCGCGGGTATAGTTTAGTGGTAGAACCTCAGCCTTCCAAGCTGATGATGCGGGTTCGATTCCCGCTACCCGCTCCAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAgg                                                                                                                                          <  1:165740/149‑1 (MQ=255)           cgcgGGTATAGTTTAGTGGTAGAACCTCAGCCTTCCAAGCTGATGATGCGGGTTCGATTCCCGCTACCCGCTCCAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGGGTGAg                                                                                                                                     <  6:233287/149‑1 (MQ=255)           cgcgGGTATAGTTTAGTGGTAGAACCTCAGCCTTCCAAGCTGATGATGCGGGTTCGATTCCCGCTACCCGCTCCAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTACCAAGGGTgtg                                                                                                                                     <  1:193767/149‑3 (MQ=255)           cgcgGGTATAGTTTAGTGGTAGAACCTCAGCCTTCCAAGCTGATGATGCGGGTTCGATTCCCGCTACCCGCTCCAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTACCAAGGGTgtg                                                                                                                                     <  6:67238/149‑3 (MQ=255)           cgcgGGTATAGTTTAGTGGTAGAACCTCAGCCTTCCAAGCTGATGATGCGGGTTCGATTCCCGCTACCCGCTCCAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGATGGTAGAGCACACCCTTGGTAAGGGTGa                                                                                                                                      <  1:201886/148‑1 (MQ=255)            gcgGGTATAGTTTAGTGGTAGAACCTCAGCCTTCCAAGCTGATGATGCGGGTTCGATTCCCGCTACCCGCTCCAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGGGTGAgg                                                                                                                                    >  1:213120/1‑149 (MQ=255)                gTATAGTTTAGTGGTAGAACCTCAGCCTTCCAAGCTGATGATGCGGGTTCGATTCCCGCTACCCGCTCCAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAg                                                                                                                                <  7:51321/149‑1 (MQ=255)                gTATAGTTTAGTGGTAGAACCTCAGCCTTCCAAGCTGATGATGCGGGTTCGATTCCCGCTACCCGCTCCAAGTTTGTAGGTTTTTGAAAAAAGTGTTTCGGTGTTGTAGGGAAGTTGGTAGAGAACACCCTGGGTATGGGGGGGGTGAg                                                                                                                                >  8:51321/1‑149 (MQ=255)                    aGTTTAGTGGTAGAACCTCAGCCTTCCAAGCTGATGATGCGGGTTCGATTCCCGCTACCCGCTCCAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGt                                                                                                                            >  1:291580/1‑149 (MQ=255)                      tttAGTGGTAGACCCTCAGCCTTCCAAGCTGATGATGCCGGTTCGATTACCCCTACCCCCTCCAAGTTAGACGTATTTTGAACAAAGTGATACGCTATTGTAGCTCAGTTGGTAGAGCACACCCTTGCGAAGCGATATGTAAGCGGtt                                                                                                                           >  8:245962/1‑148 (MQ=255)                      tttAGTGGTAGAACCTCATCCTTACAAGCTTATGATTCGGGTTCGTTTCCCTCTACCCGGTCCAAGTTTGTCGGATTTTGCTCAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGATCACAGCCTTGGTAAGGGTGAGGTCAGCGGtt                                                                                                                           <  7:245962/148‑1 (MQ=255)                         aGTGGTAGAACCTCAGCCTTCCAAGCTGATGATGCGGGTTCGATTCCCGCTACCCGCTCCAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCaa                                                                                                                        <  3:9704/148‑1 (MQ=255)                         aGTGGTAGAACCTCAGCCTTCCAAGCTGATGATGCGGGTTCGATTCCCGCTACCCGCTCCAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCaa                                                                                                                        <  7:173825/148‑1 (MQ=255)                          gTGGTAGAACCTCAGCCTTCCAAGCTGATGATGCGGGTTCGATTCCCGCTACCCGCTCCAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGt                                                                                                                      >  6:359634/1‑149 (MQ=255)                             gTAGAACCTCAGCCTTCCAAGCTGATGATGCGGGTTCGATTCCCGCTACCCGCTCCAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCg                                                                                                                   >  1:212949/1‑149 (MQ=255)                                 aaCCTCAGCCTTCCAAGCTGATGATGCGGGTTCGATTCCCGCTACCCGCTCCAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTc                                                                                                                >  2:337949/1‑148 (MQ=255)                                  aCCTCAGCCTTCCAAGCTGATGATGCGGGTTCGATTCCCGCTACCCGCTCCAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCaa                                                                                                              <  5:359634/149‑1 (MQ=255)                                  aCCTCAGCCTTCCAAGCTGATGATGCGGGTTCGATTCCCGCTACCCGCTCCAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCaa                                                                                                              <  3:40906/149‑1 (MQ=255)                                   ccTCAGCCTTCCAAGCTGATGATGCGGGTTCGATTCCCGCTACCCGCTCCAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTc                                                                                                                >  1:8681/1‑146 (MQ=255)                                       aGCCTTCCAAGCTGATGATGCGGGTTCGATTCCCGCTACCCGCTCCAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAagag                                                                                                           <  7:26768/147‑1 (MQ=255)                                           ttCCAAGCTGATGATGCGGGTTCGATTCCCGCTACCCGCTCCAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCa                                                                                                      >  6:82984/1‑148 (MQ=255)                                             ccAAGCTGATGATGCGGGTTCGATTCCCGCTACCCGCTCCAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGGGTGAGGGCAGCGGTTCAAGTCCCCTCAAGAGCTCCat                                                                                                     <  7:310467/147‑1 (MQ=255)                                              cAAGCTGATGATGCGGGTTCGATTCCCGCTACCCGCTCCAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCatat                                                                                                   <  2:213120/148‑1 (MQ=255)                                              cAAGCTGATGATGCGGGTTCGATTCCCGCTACCCGCTCCAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCatat                                                                                                   >  5:309954/1‑148 (MQ=255)                                              cAAGCTGATGATGCGGGTTCGATTCCCGCTACCCGCTCCAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCAtata                                                                                                  <  1:140036/149‑1 (MQ=255)                                                     atgatgCGGGTTCGATTCCCGCTACCCGCTCCAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACaa                                                                                             <  6:309954/147‑1 (MQ=255)                                                     atgatgCGGGTTCGATTCCCGCTACCCGCTCCAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACaa                                                                                             <  2:8681/147‑1 (MQ=255)                                                     atgatgCGGGTTCGATTCCCGCTACCCGCTCCAAGTTTGTCGCATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCTGTTCAAGTCCGCTCAAGAGCTCCATATAAACAAg                                                                                            <  1:228096/148‑1 (MQ=255)                                                      tgatgCGGGTTCGATTCCCGCTACCCGCTCCAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAAGGc                                                                                          <  2:212949/149‑1 (MQ=255)                                                          gCGGGTTCGATTCCCGCTACCCGCTCCAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAaga                                                                                                            >  5:1747/1‑127 (MQ=255)                                                          gCGGGTTCGATTCCCGCTACCCGCTCCAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAaga                                                                                                            <  6:1747/127‑1 (MQ=255)                                                            gggTTCGATTCCCGCTACCCGCTCCAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAAGGCAGatat                                                                                    >  5:147329/1‑149 (MQ=255)                                                               ttCGATTCCCGCTACCCGCTCCAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAAGGCAGATATGa                                                                                  >  2:661/1‑148 (MQ=255)                                                                   aTTCCCGCTACCCGCTCCAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCaa                                                                                                              >  8:131697/1‑116 (MQ=255)                                                                   aTTCCCGCTACCCGCTCCAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCaa                                                                                                              <  7:131697/116‑1 (MQ=255)                                                                        cGCTACCCGCTCCAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTc                                                                                                                     <  6:137720/104‑1 (MQ=255)                                                                        cGCTACCCGCTCCAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTc                                                                                                                     >  5:137720/1‑104 (MQ=255)                                                                         gCTACCCGCTCCAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAAGGCAGATATTTTCATATCTGcc                                                                       <  1:133333/149‑1 (MQ=255)                                                                              ccGCTCCAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAAGGCAGATATGAAAATATCTGCCTTTGt                                                                  <  3:41347/149‑1 (MQ=255)                                                                                   ccAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAAGGCAGata                                                                                     >  3:341292/1‑125 (MQ=255)                                                                                   ccAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAAGGCAGata                                                                                     <  4:341292/125‑1 (MQ=255)                                                                                   ccAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAAGGCAGATATGAAAATATCTGCCTTTGtt                                                                 <  3:329960/145‑1 (MQ=255)                                                                                   ccAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAAGGCAGATATGAAAATATCTGCCTTTGtt                                                                 >  4:329960/1‑145 (MQ=255)                                                                                    cAAGTTTGTCTGATTTTGCACATAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGGTCTAGTCCGCTCAAGAGCTCCATATAAACTAGGCAGATATGAAAATATCTGCCTTTGTTTTa                                                              <  6:213038/147‑1 (MQ=255)                                                                                            tCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAAGGCAGATATGAAAATATCTGCCTTTGTTTTa                                                              >  3:26883/1‑139 (MQ=255)                                                                                            tCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAAGGCAGATATGAAAATATCTGCCTTTGTTTTa                                                              <  4:26883/139‑1 (MQ=255)                                                                                               gATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAAGGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGAc                                                  >  5:59530/1‑148 (MQ=255)                                                                                               gATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAAGGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGAc                                                  >  3:88420/1‑148 (MQ=255)                                                                                               gATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAAGGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACt                                                 <  2:291580/149‑1 (MQ=255)                                                                                                       acaAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAAGGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACTATttcttc                                         >  8:183125/1‑149 (MQ=255)                                                                                                       acaAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAAGGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACTATttcttc                                         >  2:26439/1‑149 (MQ=255)                                                                                                        caAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAAGGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACTATTtcttct                                        >  5:426/1‑149 (MQ=255)                                                                                                        caAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAAGGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACTATTtcttct                                        <  6:147329/149‑1 (MQ=255)                                                                                                                   gCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAAGGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCaa                                                     <  6:49309/125‑1 (MQ=255)                                                                                                                   gCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAAGGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCaa                                                     >  5:49309/1‑125 (MQ=255)                                                                                                                    ctctTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAAGGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACTATTTCTTCTGCGGAGGATTGt                            <  1:337949/149‑1 (MQ=255)                                                                                                                    ctctTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAAGGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACTATTTCTTCTGCGGAGGATTGt                            <  5:82984/149‑1 (MQ=255)                                                                                                                     tctTGTAGCTCAGTTGGTAGAGCACACACTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAAGGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACTATTTCTTCTGCGGAGGATTGTa                           >  3:54650/1‑149 (MQ=255)                                                                                                                         gTAGCTCAGTTGGTAGAGCACACCCTTTGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAAGGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACTATTTCTTCTGCGGAGGATTGTAt                          <  6:59530/146‑1 (MQ=255)                                                                                                                         gTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAAGGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACTATTTCTTCTGCGGAGGATTGTAt                          <  7:242957/146‑1 (MQ=255)                                                                                                                         gTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAAGGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACTATTTCTTCTGCGGAGGATTGTAt                          <  4:88420/146‑1 (MQ=255)                                                                                                                              tCAGTTGGTAGAGCACACACTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAAGGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAATACTAGTTCTTCTGAGGAGGATTGGATCGATGGc                   >  2:108859/1‑148 (MQ=255)                                                                                                                                 gTTGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAAGGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACTATTTCTTCTGCGGAGGATTGTATCGATGGCTAAg               <  1:661/149‑1 (MQ=255)                                                                                                                                   tGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAAGGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACTATTTCTTCTGCGGAGGATTGTATCGATGGCTAAgg              <  7:183125/148‑1 (MQ=255)                                                                                                                                           ctcacCCTTACCAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAAGGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACTATTTCTTCTGCGGAGGATTGTATCGATGGCTAAGGAAAAGttt      >  8:256925/3‑148 (MQ=255)                                                                                                                                           ctcacCCTTACCAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAAGGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACTATTTCTTCTGCGGAGGATTGTATCGATGGCTAAGGAAAAGttt      >  1:143943/3‑148 (MQ=255)                                                                                                                                           cacaCCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAAGGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACTATTTCTTCTGAggagga                                >  1:124354/1‑122 (MQ=255)                                                                                                                                           cacaCCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAAGGCAGATATGAAAATATCTGCCTTTGTTTGATCAGTGCAAGACTATTTCTTCTGCggagga                                <  2:124354/122‑1 (MQ=255)                                                                                                                                            acacCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAAGGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACTATTTCTTCTGCGGAGGATTGTATCGATGGCTAAGGAAAAGTTTGa    <  6:426/149‑1 (MQ=255)                                                                                                                                              acCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAAGGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACTATTTCTTCTGCGGAGGATTGTATCGATGGCTAAGGAAAAGTTTGATc  >  1:168360/1‑149 (MQ=255)                                                                                                                                              acCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAAGGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACTATTTCTTCTGCGGAGGATTGTATCGATGGCTAAGGAAAAGTTTGATc  >  6:222981/1‑149 (MQ=255)                                                                                                                                              acCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAAGGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACTATTTCTTCTGCGGAGGATTGTATCGATGGCTAAGGAAAAGTTTGATc  >  4:145409/1‑149 (MQ=255)                                                                                                                                               cccTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAAGGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACTATTTCTTCTGCGGAGGATTGTATCGATGGCTAAGGAAAAGTTTGa    >  7:283748/1‑146 (MQ=255)                                                                                                                                               cccTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAAGGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACTATTTCTTCTGCGGAGGATTGTATCGATGGCTAAGGAAAAGTTTGa    <  8:283748/146‑1 (MQ=255)                                                                                                                                                ccTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAAGGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACTATTTCTTCTGCGGAGGATTGTATCGATGGCTAAGGAAAAGTTTGa    >  6:114032/1‑145 (MQ=255)                                                                                                                                                ccTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAAGGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACTATTTCTTCTGCGGAGGATTGTATCGATGGCTAAGGAAAAGTTTGa    <  5:114032/145‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                              CCGCAAGCTCCGCGGGTATAGTTTAGTGGTAGAACCTCAGCCTTCCAAGCTGATGATGCGGGTTCGATTCCCGCTACCCGCTCCAAGTTTGTCGGATTTTGCACAAAGTGTTTCGCTCTTGTAGCTCAGTTGGTAGAGCACACCCTTGGTAAGGGTGAGGTCAGCGGTTCAAGTCCGCTCAAGAGCTCCATATAAACAAGGCAGATATGAAAATATCTGCCTTTGTTTTATCAGTGCAAGACTATTTCTTCTGCGGAGGATTGTATCGATGGCTAAGGAAAAGTTTGATC  >  NC_002947/532468‑532757

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑