Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 543,730 C→T 6.7% A657V (GCT→GTT)  rpoC → DNA‑directed RNA polymerase subunit beta'

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947543,7300CT 6.7% 221.3 / 3.2 74A657V (GCT→GTT) rpoCDNA‑directed RNA polymerase subunit beta'
Reads supporting (aligned to +/- strand):  ref base C (33/36);  new base T (2/3);  total (35/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTACCGCGTGGTTGGTCTGAAAGAGACCGTTATCTTCGCCGACCAACTGATGTACACCGGTTTCGCATACTCGACCATTTCCGGCGTTTCCATCGGTGTTAACGACTTCGTTATCCCGGACGAGAAAGCCCGCATCATCGGTAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTCCGGCCTGGTAACCCAGGGCGAGAAGTACAACAAGGTCATCGACTTGTGGTCGAAGGCGAACGACGAAGTGTCCAAGGCGATGATGGCCAACCTCTCGAAAGAGAAGGT  >  NC_002947/543584‑543877
                                                                                                                                                  |                                                                                                                                                   
cTACCGCGTGGTTGGTCTGAAAGAGACCGTTATCTTCGCCGACCAACTGATGTACACCGGTTTCGCATACTCGACCATTTCCGGCGTTTCCATCGGTGTTAACGACTTCGTTATCCCGGACGAGAAAGCCCGCATCATCGGTAACGCTa                                                                                                                                                   >  8:36352/1‑149 (MQ=255)
 tACCGCGTGGTTGGTCTGAAAGAGACCGTTATCTTCGCCGACCAACTGATGTACACCGGTTTCGCATACTCGACCATTTCCGGCGTTTCCATCGGTGTTAACGACTTCGTTATCCCGGACGAGAAAGCCCGCATCATCGGTAACGCTAc                                                                                                                                                  <  3:137225/149‑1 (MQ=255)
   ccGCGTGGTTGGTCTGAAAGAGACCGTTATCTTCGCCGACCCACTGATGTACACCGGTTTCGCATACTCGACCATTTCCGGCGTTTCCATCGGTGTTAACGACTTCGTTATCCCGGACGAGAAAGCCCGCATCATCGGTAACGCTAc                                                                                                                                                  <  6:17379/147‑1 (MQ=255)
   ccGCGTGGTTGGTCTGAAAGAGACCGTTATCTTCGCCGACCAACTGATGTACACCGGTTTCGCATACTCGACCATTTCCGGCGTTTCCATCGGTGTTAACGACTTCGTTATCCCGGACGAGAAAGCCCGCATCATCGGTAACGCTAc                                                                                                                                                  <  1:337010/147‑1 (MQ=255)
        tggttggtCTGAAAGAGACCGTTATCTTCGCCGACCAACTGATGTACACCGGTTTCGCATACTCGACCATTTCCGGCGTTTCCATCGGTGTTAACGACTTCGTTATCCCGGACGAGAAAGCCCGCATCATCGGTAGCGTTACCGAtgat                                                                                                                                           <  4:307332/149‑2 (MQ=255)
        tggttggtCTGAAAGAGACCGTTATCTTCGCCGACCAACTGATGTACACCGGTTTCGCATACTCGACCATTTCCGGCGTTTCCATCGGTGTTAACGACTTCGTTATCCCGGACGAGAAAGCCCGCATCATCGGTAACGCTACCGAtgaa                                                                                                                                           <  3:302325/149‑1 (MQ=255)
          gttggtCTGTAACCCTCCCTTATCTTCGCCTTCCAACTTTTGTTCTCCCGTTTCGCATTATCCTCCATTTCCGGCGTTTACATCGGTGTTAACGACTTCGTTATCCCGGACTAGAAAGCCCGCATCATCGGTAACGCTACCGAtgatgt                                                                                                                                         <  8:317289/149‑1 (MQ=255)
          gttggtCTGAAAGAGACCGTTATCTTCGCCGACCAACTGATGTACACCGGTTTCGCATACTCGACCATTTCCGGCGTTTCCATCGGTGTTAACGACTTCGTTATCCCGGACGAGAAAGCCCGCATCATCGGTAACGCTACCGAtgaagt                                                                                                                                         <  5:304363/149‑1 (MQ=255)
              gtCTGAAAGAGACCGTTATCTTCGCCGACCAACTGATGTACACCGGTTTCGCATACTCGACCATTTCCGGCGTTTCCATCGGTGTTAACGACTTCGTTATCCCGGACGAGAAAGCCCGCATCATCGGTAACGCTACCGAtgaagtgaa                                                                                                                                      <  4:30845/148‑1 (MQ=255)
              gtCTGAAAGAGACCGTTATCTTCGCCGACCAACTGATGTACACCGGTTTCGCATACTCGACCATTTCCGGCGTTTCCATCGGTGTTAACGACTTCGTTATCCCGGACGAGAAAGCCCGCATCATCGGTAACGCTACCGAtgaagtgaa                                                                                                                                      <  4:74064/148‑1 (MQ=255)
               tCTGAAAGAGACCGTTATCTTCGCCGACCAACTGATGTACACCGGTTTCGCATACTCGACCATTTCCGGCGTTTCCATCGGTGTTAACGACTTCGTTATCCCGGACGAGAAAGCCCGCATCATCGGTAGCGTTACCGAtg                                                                                                                                             <  8:119885/140‑1 (MQ=255)
               tCTGAAAGAGACCGTTATCTTCGCCGACCAACTGATGTACACCGGTTTCGCATACTCGACCATTTCCGGCGTTTCCATCGGTGTTAACGACTTCGTTATCCCGGACGAGAAAGCCCGCATCATCGGTAGCGTTACCGAtg                                                                                                                                             >  7:119885/1‑140 (MQ=255)
                        gaCCGTTATCTTCGCCGACCTACTGATTTACACCGGTTTCGCATACTCGACCATTTCCGGCGTTTCCATCGGTGTTAACTACTTCGTTATCCCGGACGATAAAGCCCGCATCATCGGTAACGCTACCGATGAAGTGAAGGAAATCgaga                                                                                                                           <  1:267779/149‑1 (MQ=255)
                                            taCTGATGTACACCGGTTTCGCATACTCGACCATTTCCGGCGTTTCCATCGGTGTTAACGACTTCGTTATCCCGGACGAGAAAGCCCGCATCATCGGTAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTCCGGCCTg                                                                                                       <  3:143593/148‑1 (MQ=255)
                                            aaCTGATGTACACCGGTTTCGCATACTCGACCATTTCCGGCGTTTCCATCGGTGTTAACGACTTCGTTATCCCGGACGAGAAAGCCCGCATCATCGGTAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTCCGGCCTg                                                                                                       <  7:191522/149‑1 (MQ=255)
                                              cTGATGTACACCGGTTTCGCATACTCGACCATTTCCGGCGTTTCCATCGGTGTTAACGACTTCGTTATCCCGGACGAGAAAGCCCGCATCATCGGTAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTCCGGCCTg                                                                                                       >  4:143593/1‑147 (MQ=255)
                                                   gTACACCGGTTTCGCATACTCGACCATTTCCGGCGTTTCCATCGGTGTTAACGACTTCGTTATCCCGGACGAGAAAGCCCGCATCATCGGTAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTCCGGCCTGGTAAcc                                                                                                 >  8:29278/1‑148 (MQ=255)
                                                     acacCGGTTTCGCATACTCGACCATTTCCGGCGTTTCCATCGGTGTTAACGACTTCGTTATCCCGGACGAGAAAGCCCGCATCATCGGTAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTCCGGCCTGGTAACCCAg                                                                                              >  6:229607/1‑149 (MQ=255)
                                                      cacCGGTTTCGCATACTCGACCATTTCCGGCGTTTCCATCGGTGTTAACGACTTCGTTATCCCGGACGAGAAAGCCCGCATCATCGGTAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTCCGGCCTGGTAACCCAgg                                                                                             <  2:320896/149‑1 (MQ=255)
                                                       acCGGTTTCGCATACTCGACCATTTCCGGCGTTTCCATCGGTGTTAACGACTTCGTTATCCCGGACGAGAAAGCCCGCATCATCGGTAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTCCGGCCTGGTAACCCAgg                                                                                             <  6:166487/148‑1 (MQ=255)
                                                       acCGGTTTCGCATACTCGACCATTTCCGGCGTTTCCATCGGTGTTAACGACTTCGTTATCCCGGACGAGAAAGCCCGCATCATCGGTAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTCCGGCCTGGTAACCCAgg                                                                                             >  5:166487/1‑148 (MQ=255)
                                                         cGGTTTCGCATACTCGACCATTTCCGGCGTTTCCATCGGTGTTAACGACTTCGTTATCCCGGACGAGAAAGCCCGCATCATCGGTAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTCCGGCCTg                                                                                                       <  1:196960/136‑1 (MQ=255)
                                                         cGGTTTCGCATACTCGACCATTTCCGGCGTTTCCATCGGTGTTAACGACTTCGTTATCCCGGACGAGAAAGCCCGCATCATCGGTAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTCCGGCCTg                                                                                                       >  2:196960/1‑136 (MQ=255)
                                                         cGGTTTCGCATACTCGACCATTTCCGGCCTTTCCATCGGTGTTAACGACTTCGTTATCCCCGACGAGAAAGCCAGCATCATCCGTAAAGCAACCGAAGAAGTGAAGGAAATCGAGAGCCAGAACGCCTCCGGCCTGCTAACCCAgcgc                                                                                           >  1:61838/1‑148 (MQ=255)
                                                           gTTTCGCATACTCGACCATTTCCGGCGTTTCCATCGGTGTTAACGACTTCGTTATCCCGGACGAGAAAGCCCGCATCATCGGTAACGCTACCGATGAAGTGAAGGAAATCga                                                                                                                             >  4:186245/1‑112 (MQ=255)
                                                               cGCATACTCGACCATTTCCGGCGTTTCCATCGGTGTTAACGACTTCGTTATCCCGGACGAGAAAGCCCGCATCATCGGTAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTCCGGCCTGGTAACCCAGGGCGAGAAg                                                                                     <  4:55235/148‑1 (MQ=255)
                                                                gCATACTCGACCATTTCCGGCGTTTCCATCGGTGTTAACGACTTCGTTATCCCGGACGAGAAAGCCCGCATCATCGGTAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTCCGGCCTGGTAACCCAGGGCGAGAAGTa                                                                                   <  7:29278/149‑1 (MQ=255)
                                                                gCATACTCGACCATTTCCGGCGTTTCCATCGGTGTTAACGACTTCGTTATCCCGGACGAGAAAGACCGCATCATCGGTAACGCTACCGATGAAGTGAAGGAAATCgaga                                                                                                                           >  2:147963/1‑109 (MQ=255)
                                                                gCATACTCGACCATTTCCGGCGTTTCCATCGGTGTTAACGACTTCGTTATCCCGGACGAGAAAGACCGCATCATCGGTAACGCTACCGATGAAGTGAAGGAAATCgaga                                                                                                                           <  1:147963/109‑1 (MQ=255)
                                                                 cATACTCGACCATTTCCGGCGTTTCCATCGGTGTTAACGACTTCGTTATCCCGGACGAGAAAGCCCGCATCATCGGTAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTCCGGCCTGGTAACCCAGGGc                                                                                           <  5:121072/140‑1 (MQ=255)
                                                                 cATACTCGACCATTTCCGGCGTTTCCATCGGTGTTAACGACTTCGTTATCCCGGACGAGAAAGCCCGCATCATCGGTAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTCCGGCCTGGTAACCCAGGGc                                                                                           >  6:121072/1‑140 (MQ=255)
                                                                 cATACTCGACCATTTCCGGCGTTTCCATCGGTGTTAACGACTTCGTTATCCCGGACGAGAAAGCCCGCATCATCGGTAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTCCGGCCTGGTAACCCAGGGCGAGAAGTac                                                                                  <  3:8649/149‑1 (MQ=255)
                                                                    aCTCGACCATTTCCGGCGTTTCCATCGGTGTTAACGACTTCGTTATCCCGGACGAGAAAGCCCGCATCATCGGTAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTCCGGCCTGGTAACCCAGGGCGAGAAGTacaac                                                                               <  7:36352/149‑1 (MQ=255)
                                                                      tCGACCATTTCCGGCGTTTCCATCGGTGTTAACGACTTCGTTATCCCGGACGAGAAAGCCCGCATCATCGGTAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTCCGGCCTGGTAACCCAGGGCGAGAAGTacaac                                                                               >  4:264421/1‑147 (MQ=255)
                                                                        gACCATTTCCGGCGTTTCCATCGGTGTTAACGACTTCGTTATCCCGGACGAGAAAGCCCGCATCATCGGTAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTCCGGCCTGGTAACCCAGGGCGAGAAGTACAACAAg                                                                            >  2:177751/1‑148 (MQ=255)
                                                                        gACCATTTCCGGCGTTTCCATCGGTGTTAACGACTTCGTTATCCCGGACGAGAAAGCCCGCATCATCGGTAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTCCGGCCTGGTAACCCAGGGCGAGAAGTACAACAAg                                                                            <  1:177751/148‑1 (MQ=255)
                                                                          ccATTTCCGGCGTTTCCATCGGTGTTAACGACTTCGTTATCACGGACGAGAAAGCCCGCATCATCGGTAACGCTACAGATGAAGTGAAGGAAATCGAGAGCAAGTACGCCTCCGGCCTCGTAACCCAGGGCGAGAAGTACAACAAgga                                                                          >  2:96952/1‑147 (MQ=255)
                                                                            ctttCCGGCGCTTCCCTCGGTGTTACCGACTTCGTTATCCCGGACGAGCAAGCCCGCCTCATCGGTAACGCTACCGATGAAGTGACCGAACTCGAGAGCCACTACGCCTCCGGCCTGGTAACCCAGGGCGAGAAGTACAACAACGTCAt                                                                       <  4:241446/148‑1 (MQ=255)
                                                                                   gCGTTTCCATCGGTGTTAACGACTTCGTTATCCCGGACGAGAAAGCCCGCATCATCGGTAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTCCGGCCTGGTAACCCAGGGCGAGAAGTACAACAAGGTCATCGACTtg                                                                <  5:229607/149‑1 (MQ=255)
                                                                                     gTTTCCATCGGTGTTAACGACTTCGTTATCCCGGACGAGAAAGCCCGCATCATCGGTAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTCCGGCCTGGTAACCCAGGGCga                                                                                         >  7:155812/1‑122 (MQ=255)
                                                                                     gTTTCCATCGGTGTTAACGACTTCGTTATCCCGGACGAGAAAGCCCGCATCATCGGTAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTCCGGCCTGGTAACCCAGGGCga                                                                                         <  8:155812/122‑1 (MQ=255)
                                                                                         ccATCGGTGTTAACGACTTCGTTATCCCGGACGAGAAAGCCCGCATCATCGGTAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTCCGGCCTGGTAACCCAGGGCGAGAAGTACAACAAGGTCATCGACTTGTGGTc                                                           >  8:90665/1‑148 (MQ=255)
                                                                                           aacGGTGTTAACGACTTCGTTATCCCGGACGAGAAAGCCCGCATCATCGGTAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTCCGGCCTGGTAACCCAGGGCGAGAAGTACAACAAGGTCATCGACTTGTGGTCg                                                          >  4:42277/3‑147 (MQ=255)
                                                                                           aTCGGTGTTAACGACTTCGTTATCCCGGACGAGAAAGCCCGCATCATCGGTAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTCCGGCCTGGTAACCCAGGGCGAGAAGTACAACAAGGTCATCGACTTGTGGTCg                                                          >  1:100448/1‑147 (MQ=255)
                                                                                           aTCGGTGTTAACGACTTCGTTATCCCGGACGAGAAAGCCCGCATCATCGGTAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTCCGGCCTGGTAACCCAGGGCGAGAAGTACAACAAGGTCATCGACTTGTGGTCGaa                                                        <  3:264421/149‑1 (MQ=255)
                                                                                           aTCGGTGTTAACGACTTCGTTATCCCGGACGAGAAAGCCCGCATCATCGGTAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTCCGGCCTGGTAACCCAGGGCGAGAAGTACAACAAGGTCATCGACTTGTGGTCGaa                                                        <  3:42277/149‑1 (MQ=255)
                                                                                           aTCGGTGTTAACGACTTCGTTATCCCGGACGAGAAAGCCCGCATCATCGGTAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTCCGGCCTGGTAACCAAGGGCGAGAAGTACAACAAGGTCATCGACTTGTGGTCg                                                          >  5:95186/1‑147 (MQ=255)
                                                                                                 gtTAACGACTTCGTTATCCCGGACGAGAAAGCCCGCATCATCGGTAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTCCGGCCTGGTAACCCAGGGCGAGAAg                                                                                     >  5:333562/1‑114 (MQ=255)
                                                                                                 gtTAACGACTTCGTTATCCCGGACGAGAAAGCCCGCATCATCGGTAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTCCGGCCTGGTAACCCAGGGCGAGAAg                                                                                     <  6:333562/114‑1 (MQ=255)
                                                                                                  ttAACGACTTCGTTATCCCGGACGAGAAAGCCCGCATCATCGGTAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTCCGGCCTGGTAACCCAGGGCGAGAAGTACAACAAGGTCATCGACTTGTGGTCGAAGGCGaa                                                  <  7:90665/148‑1 (MQ=255)
                                                                                                          ttCGTTATCCCGGACGAGAAAGCCCGCATCATCGGTAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTCCGGCCTg                                                                                                       <  7:124785/87‑1 (MQ=255)
                                                                                                          ttCGTTATCCCGGACGAGAAAGCCCGCATCATCGGTAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTCCGGCCTg                                                                                                       >  8:124785/1‑87 (MQ=255)
                                                                                                          ttCGTTATCCCGGACGAGAAAGCCCGCATCATCGGTAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTCCGGCCTGGTAACCCAGGGCGAGAAGTACAACAAGGTCATCGACTTGTGGTCGAAGGCGAACGACGAAgt                                         <  1:96952/149‑1 (MQ=255)
                                                                                                              ttATCCCGGACGAGAAAGCCCGCATCATCGGTAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTCCGGCCTGGTAACCCAGGGCGAGAAGTACAACAAGGTCATCGACTtg                                                                >  6:19224/1‑122 (MQ=255)
                                                                                                              ttATCCCGGACGAGAAAGCCCGCATCATCGGTAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTCCGGCCTGGTAACCCAGGGCGAGAAGTACAACAAGGTCATCGACTTGTGGTCGAAGGCGAACGACGAAGTGTcc                                     <  2:100448/149‑1 (MQ=255)
                                                                                                                   cctgACGAGAAAGCCCGCATCATAGGTAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGACTACGGCCTGGTAAACCAGGGCGAGAAGTACAACAAGGTCATCGACTTGTGGTCTAAGGAGAACGAAGAAGTGTACAAGGc                                >  6:204681/4‑149 (MQ=255)
                                                                                                                   ccGGACGAGAAAGCCCGCATCATCGGTAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTCCGGCCTGGTAACCCAGGGCGAGAAGTACAACAAGGTCATCGACTTGTGGTCGCAGGAGAACGACGAAGTCTCAAAGGc                                >  8:323400/1‑149 (MQ=255)
                                                                                                                   ccGGACGAGAAAGCCCGCATCATCGGTAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTCCGGCCTGGTAACCCAGGGCGAGAAGTACAACAAGGTCATCGACTTGTGGTCGAAGGCGAACGACGAAGTGTCCAAGGc                                >  8:327429/1‑149 (MQ=255)
                                                                                                                         gagaAAGCCCGCATCATCGGTAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTCCGGCCTGGTAACCCAGGGCGAGAAGTACAAGAAGGTCATCGACTTGTGGTCGAAGGCGAACGACGAAGTGTCCAAGGCgatgat                          >  1:296353/1‑149 (MQ=255)
                                                                                                                         gagaAAGCCCGCATCATCGGTAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTCCGGCCTGGTAACCCAGGGCGAGAAGTACAACAAGGTCATCGACTtg                                                                <  4:3665/111‑1 (MQ=255)
                                                                                                                         gagaAAGCCCGCATCATCGGTAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTCCGGCCTGGTAACCCAGGGCGAGAAGTACAACAAGGTCATCGACTtg                                                                >  3:3665/1‑111 (MQ=255)
                                                                                                                         gagaAAGCCCGCATCATCGGTAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTCCGGCCTGGTAACCCAGGGCGAGAAGTACAACAAGGTCATCGACTTGTGGTCGAAGGCGAACGACGAAGTGTCCAAGGCgatgat                          >  5:248498/1‑149 (MQ=255)
                                                                                                                         gagaAAGCCCGCATCATCGGTAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTCCGGCCTGGTAACCCAGGGCGAGAAGTACAACAAGGTCATCGACTTGTGGTCGAAGGCGAACGACGAAGTGTCCAAGGCga                              <  7:143848/145‑1 (MQ=255)
                                                                                                                         gagaAAGCCCGCATCATCGGTAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTCCGGCCTGGTAACCCAGGGCGAGAAGTACAACAAGGTCATCGACTTGTGGTCGAAGGCGAACGACGAAGTGTCCAAGGCga                              >  8:143848/1‑145 (MQ=255)
                                                                                                                            aaaGCCCGCATCATCGGTAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTCCGGCCTGGTAACCCAGGGCGAGAAGTACAACAAGGTCATCGACTTGTGGTCGAAGGCGAACGACGAAGTGTCCAAGGCGATGATgg                        >  1:99544/1‑148 (MQ=255)
                                                                                                                            aaaGCCCGCATCATCGGTAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTCCGGCCTGGTAACCCAGGGCGAGAAGTACAACAAGGTCATCGACTTGTGGTCGAAGGCGAACGACGAAGTGTCCAAGGCGATGATGGc                       >  1:291835/1‑149 (MQ=255)
                                                                                                                                    catcatCGGTAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTCCGGCCTGGTAACCCAGGGCGAGAAGTACAACAAGGTCATCGACTTGTGGTCGAAGGCGAACGACGAAGTGTCCAAGGCGATGATGGCCAACctct               <  2:296353/149‑1 (MQ=255)
                                                                                                                                     ttcatcGTTAGCGTTACCGATGAAGTGAATGAAATCGAGAGCCAGTACGCCTCCGGCCTGGTAACCCAGGGCGAGAAGTACAACAACGTCATCGACTTGTGGTCGAAGGCGAACGACGaa                                           <  4:359711/119‑1 (MQ=255)
                                                                                                                                     ttcatcGGTAGCGTTACCGATGATGTGAAGGAAATCGAGAGCCAGTACGACTCCGGCCTGGTAACCAAGGGCGAGAAGTACAACAAGGTCATCGACTTGTGGTCGAAGGCGAACGACGaa                                           >  3:359711/2‑120 (MQ=255)
                                                                                                                                      tcatcGGTAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTCCGGCCTGGTAACCCAGGGCGAGAAGTACAACAAGGTCATCGACTTGTGGTCGAAGGCGAACGACGAAGTGTCCAAGGCGATGATGGCCAACCtctc              >  2:303969/1‑148 (MQ=255)
                                                                                                                                         tcGGTAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTCCGGCCTGGTAACCCAGGGCGAGAAGTACAACAAGGTCATCGACTTGTGGTCGAAGGCGAACGACGAAGTGTCCAAGGCGATGATGGCCAACCTCTCGaaa          <  1:251673/149‑1 (MQ=255)
                                                                                                                                            gTAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTCCGGCCTGGTAACCCAGGGCGAGAAGTACAACAAGGTCATCGACTTGTGGTCGAAGGCGAACGACGAAGTGTCCAAGGCGATGATGGCCAACCTCTCGaaa          >  1:100664/1‑146 (MQ=255)
                                                                                                                                             tAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTCCGGCCTGGTAACCCAGGGCGAGAAGTACAACAAGGTCATCGACTTGTGGTCGAAGGCGAACGACGAAGTGTCCAAGGCGATGATGGCCAACCTCTCGAAAgaga      <  6:248498/149‑1 (MQ=255)
                                                                                                                                                 gCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTCCGGCCTGGTAACCCAGGGCGAGAAGTACAACAAGGTCATCGACTTGTGGTCGAAGGCGAACGACGAAGTGTCCAAGGCGATGATGGCCAACCTCTCGAAAGAGAAGGt  >  7:283336/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
CTACCGCGTGGTTGGTCTGAAAGAGACCGTTATCTTCGCCGACCAACTGATGTACACCGGTTTCGCATACTCGACCATTTCCGGCGTTTCCATCGGTGTTAACGACTTCGTTATCCCGGACGAGAAAGCCCGCATCATCGGTAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTCCGGCCTGGTAACCCAGGGCGAGAAGTACAACAAGGTCATCGACTTGTGGTCGAAGGCGAACGACGAAGTGTCCAAGGCGATGATGGCCAACCTCTCGAAAGAGAAGGT  >  NC_002947/543584‑543877

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑