Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 563,007 T→G 5.2% P30P (CCT→CCG) ‡ rpoA → DNA‑directed RNA polymerase subunit alpha

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947563,0070TG 5.2% 249.5 / 2.8 77P30P (CCT→CCG) ‡rpoADNA‑directed RNA polymerase subunit alpha
Reads supporting (aligned to +/- strand):  ref base T (36/37);  new base G (2/2);  total (38/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.65e-01

AACGAAAACCTGATTGTCGAGCTCTACTCCAAGTAAGGGCTAGAAAATAGGTGCATCCATGCAGATTTCGGTAAATGAGTTCCTGACTCCCCGCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATGCCTGGCTGTGCAGTAGTCGAGGCCGAGATCGATGGCGTACTCCATGAGTACTCCGCGATCGAAGGTGTTCAGGAAGACG  >  NC_002947/562860‑563149
                                                                                                                                                   |                                                                                                                                              
aaCGAAAACCTGATTGTCGAGCTCTACTCCAAGTAAGGGCTAGAAAATAGGTGCATCCATGCAGATTTCGGTAAATGAGTTCCTGACTCCCCGCCACATTGATGTTCAGGTAGTAAGTCCGACAAGCGCCAAAATTACGCTCGAGCct                                                                                                                                                >  8:343380/1‑148 (MQ=255)
 aCGAAAACCTGATTGTCGAGCTCTACTCCAAGTAAGGGCTAGAAAATAGGTGCATCCATGCAGATTTCGGTAAATGAGTTCCTGACTCCCCGCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCctct                                                                                                                                              >  6:202247/1‑149 (MQ=255)
    aaaaCCTGATTGTCGAGCTCTACTCCAAGTAAGGGCTAGAAAATAGGTGCATCCATGCAGATTTCGGTAAATGAGTTCCTGACTCCCCGCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGa                                                                                                                                           <  6:320936/149‑1 (MQ=255)
         cTGATTGTCGAGCTCTACTCCAAGTAAGGGCTAGAAAATAGGTGCATCCATGCAGATTTCGGTAAATGAGTTCCTGACTCCCCGCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCGAAACCACGCTCGAGAGGCTCGAGCGt                                                                                                                                       >  4:307661/1‑148 (MQ=255)
            aTTGTCGAGCTCTACTCCAAGTAACGGCTAGAAAATAGGTGCATCCATGCAGATTTCGGTAAATGACTTCCTGACTCCCCGCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGAGGCTCGAGCGtaat                                                                                                                                    <  2:125500/148‑4 (MQ=255)
                tCGAGCTCTACTCCAAGTAAGGGCTAGAAAATAGGTGCATCCATGCAGATTTCGGTAAATGAGTTCCTGACTCCCCGCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCgg                                                                                                                               <  3:108680/149‑1 (MQ=255)
                tCGAGCTCTACTCCAAGTAAGGGCTAGAAAATAGGTGCATCCATGCAGATTTCGGTAAATGAGTTCCTGACTCCCCGCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCg                                                                                                                                <  7:180482/148‑1 (MQ=255)
                   aGCTCTACTCCAAGTAAGGGCTAGAAAATAGGTGCATCCATGCAGATTTCGGTAAATGAGTTCCTGACTCCCCGCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCGAAACCACGCTCGAGAGGCTCGAGCGTAATTTTGGcgc                                                                                                                            <  3:307661/149‑3 (MQ=37)
                       ctACTCCAAGTAAGGGCTAGAAAATAGGTGCATCCATGCAGATTTCGGTAAATGAGTTCCTGACTCCCCGCCACATTGATGTGCAGGTAGTCAGTCCTACCCTCACCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATAcc                                                                                                                        >  3:77522/1‑149 (MQ=255)
                          cTCCAAGTAAGGGCTAGAAAATAGGTGCATCCATGCAGATTTCGGTAAATGAGTTCCTGACTCCCCGCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTg                                                                                                                     >  4:66382/1‑149 (MQ=255)
                           tCCAAGTAAGGGCTAGAAAATAGGTGCATCCATGCAGATTTCGGTAAATGAGTTCCTGACTCCCCGCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTgg                                                                                                                    >  1:130526/1‑149 (MQ=255)
                           tCCAAGTAAGGGCTAGAAAATAGGTGCATCCATGCAGATTTCGGTAAATGAGTTCCTGACTCCCCGCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTgg                                                                                                                    >  5:321717/1‑149 (MQ=255)
                           tCCAAGTAAGGGCTAGAAAATAGGTGCATCCATGCAGATTTCGGTAAATGAGTTCCTGACTCCCCGCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTgg                                                                                                                    >  5:155357/1‑149 (MQ=255)
                             cAAGTAAGGGCTAGAAAATAGGTGCATCCATGCAGATTTCGGTAAATGAGTTCCTGACTCCCCGCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTggg                                                                                                                   >  6:495/1‑148 (MQ=255)
                             cAAGTAAGGGCTAGAAAATAGGTGCATCCATGCAGATTTCGGTAAATGAGTTCCTGACTCCCCGCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTgg                                                                                                                    <  5:202247/147‑1 (MQ=255)
                                gTAAGGGCTAGAAAATAGGTGCATCCATGCAGATTTCGGTAAATGAGTTCCTGACTCCCCGCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATAcc                                                                                                                        >  3:202228/1‑140 (MQ=255)
                                gTAAGGGCTAGAAAATAGGTGCATCCATGCAGATTTCGGTAAATGAGTTCCTGACTCCCCGCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATAcc                                                                                                                        <  4:202228/140‑1 (MQ=255)
                                      gCTAGAAAATAGGTGCATCCATGCAGATTTCGGTAAATGAGTTCCTGACTCCCCGCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTg                                                                                                         >  7:39514/1‑149 (MQ=255)
                                        tAGAAAATAGGTGCATCCATGCAGATTTCGGTAAATGAGTTCCTGACTCCCCGCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTgcg                                                                                                       >  7:305607/1‑149 (MQ=255)
                                        tAGAAAATAGGTGCATCCATGCAGATTTCGGTAAATGAGTTCCTGACTCCCCGCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTgcg                                                                                                       >  1:94847/1‑149 (MQ=255)
                                             aaTAGGTGCATCCATGCAGATTTCGGTAAATGAGTTCCTGACTCCCCGCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCa                                                                                                  >  2:71907/1‑149 (MQ=255)
                                               tAGGTGCATCCATGCAGATTTCGGTAAATGAGTTCCTGACTCCCCGCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATc                                                                                                <  5:495/149‑1 (MQ=255)
                                                     cATCCATGCAGATTTCGGTAAATGAGTTCCTGACTCCCCGCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCtgttg                                                                                          <  7:343380/149‑1 (MQ=255)
                                                           tGCAGATTTCGGTAAATGAGTTCCTGACTCCCCGCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTTTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGtcct                                                                                      <  4:41723/147‑1 (MQ=255)
                                                           tGCAGATTTCGGTAAATGAGTTCCTGACTCCCCGCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTGTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGtcct                                                                                      >  3:41723/1‑147 (MQ=255)
                                                           tGCAGATTTCGGTAAATGAGTTCCTGACTCCCCGCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGtcctc                                                                                     >  2:364107/1‑148 (MQ=255)
                                                              aGATTTCGGTAAATGAGTTCCTGACTCCCCGCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGtcctcc                                                                                    <  2:54006/146‑1 (MQ=255)
                                                              aGATTTCGGTAAATGAGTTCCTGACTCCCCGCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGtcctcc                                                                                    >  1:54006/1‑146 (MQ=255)
                                                                 tttCGGTAAATGAGTTCCTGACTCCCCGCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATGCCt                                                                              <  2:244867/149‑1 (MQ=255)
                                                                         aaTGAGTTCCTGACTCCCCGCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATGCCTGGCTGTGc                                                                      <  1:71907/149‑1 (MQ=255)
                                                                          aTGAGTTCCTGACTCCCCGCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATGCCTGGCtgtg                                                                       >  5:5863/1‑147 (MQ=255)
                                                                          aTGAGTTCCTGACTCCCCGCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATGCCTGGCTGTGCa                                                                     <  5:201703/149‑1 (MQ=255)
                                                                          aTGAGTTCCTGACTCCCCGCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATGCCTGGCTGTGCa                                                                     <  8:39514/149‑1 (MQ=255)
                                                                                 ccTGACTCCCCGCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATGCCTGGCTGTGCAGTAGTc                                                               >  2:320261/1‑148 (MQ=255)
                                                                                  cTGACTCCCCGCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATGCCTGGCTGTGCAGTAGTc                                                               >  1:196197/1‑147 (MQ=255)
                                                                                  cTGACTCCCCGCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATGCCTGGCTGTGCAGTAGTCGa                                                             <  2:130526/149‑1 (MQ=255)
                                                                                  cTGACTCCCCGCCACATTGATGTGCAGGTAGTCAGTCAGACACGCGCCAAAATTACGCTAGAGCCTCTCGAGCGAGGTTTCGGCCATACCCAGGGCAACGCGCTGCGCCGCATCCTCTTGTCCTCCATGCCTGGCTGTGCAGTAGTCGa                                                             >  1:227487/1‑149 (MQ=255)
                                                                                   tGACTCCCCGCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCTTGTTGTCCTCCATGCCTGGCTGTGCAGTAGTCGAg                                                            >  6:217329/1‑149 (MQ=255)
                                                                                       tCCCCGCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATGCCTGGCTGTGCAGTAGTCGAGGcc                                                         >  4:255519/1‑148 (MQ=255)
                                                                                         cccGCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATGCCTGGCTGTGCAGTAGTCGAGGcc                                                         >  3:163758/1‑146 (MQ=255)
                                                                                         cccGCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATGCCTGGCTGTGCAGTAGTCGAGGCCgag                                                      >  4:240767/1‑149 (MQ=255)
                                                                                          ccGCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATGCCTGGCTGTGCAGTAGTCGAGGCCgaga                                                     <  4:163758/149‑1 (MQ=255)
                                                                                           cGCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATGCCTGGCTGTGCAGTAGTCGAGGcc                                                         >  1:109679/1‑144 (MQ=255)
                                                                                           cGCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATGCCTGGCTGTGCAGTAGTCGAGGCCGAGAt                                                    <  3:240767/149‑1 (MQ=255)
                                                                                            gCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATGCCTGGCTGTGCAGTAGTCGAGGcc                                                         >  7:184648/1‑143 (MQ=255)
                                                                                              cacaTTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATGCCTGGCTGTGCAGTAGTCGAGGCCGAGATCGa                                                 >  2:351636/1‑149 (MQ=255)
                                                                                               acaTTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATGCCTGGCTGTGCAGTAGTCGAGGCCGAGATCGAt                                                <  8:184648/149‑1 (MQ=255)
                                                                                                caTTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATGCCTGGCTGTGCAGTAGTCGAGGCCGAGATCGATg                                               <  2:196197/149‑1 (MQ=255)
                                                                                                 aTTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATGCCTGGCTGTGCAGTAGTCGAGGCCGAGATCGATgg                                              >  2:251971/1‑149 (MQ=255)
                                                                                                  ttGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATGCCTGGCTGTGCAGTAGTCGAGGCCGAGATCGATGGc                                             <  1:364107/149‑1 (MQ=255)
                                                                                                    gATGTGCATGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCTAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATGCCTGGCTGTGCAGTAGTCGAGGCCGAGATCGATGGCg                                            <  2:227487/148‑1 (MQ=255)
                                                                                                        tgCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATGCCTGGCTGTGCAGTAGTCGAGGCCGAGATCGATGGCGTACTc                                       <  3:66382/149‑1 (MQ=255)
                                                                                                           aGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATGCCTGGCTGTGCAGTAGTCGAGGCCGAGATCGATGGCGTACTCCAt                                    <  1:251971/149‑1 (MQ=255)
                                                                                                             gTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATGCCTGGCTGTGCAGTAGTCGAGGCCGAGATCGATGGCGTACTCCAt                                    >  6:188045/1‑147 (MQ=255)
                                                                                                              tAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATGCCTGGCTGTGCAGTAGTCGAGGCCGAGATCGATGGCGTACTCCATGAg                                 >  2:204567/1‑149 (MQ=255)
                                                                                                                 tcagtcCGACCCGCGCCAAAATTACGCTCGAGACTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATGCCTGGCTGTGCAGTAGTCGAGGCCGAGATCGATGGCGTACTCCATGAGTAc                              >  6:10624/1‑149 (MQ=255)
                                                                                                                     tcCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCTTGTTGTCCTCCATGCCTGGCTGTGCAGTAGTCGAGGCCGAGATCGATGGCGTACTCCATGAGTACTCcg                          <  5:217329/149‑1 (MQ=255)
                                                                                                                     tcCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATGCCTGGCTGTGCAGTAGTCGAGGCCGAGATCGATGGCGTACTCCATGAGTACTCcg                          >  3:172415/1‑149 (MQ=255)
                                                                                                                        gACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATGCCTGGCTGTGCAGTAGTCGAGGCCGAGATCGATGGCGTACTCCATGAGTACTCCGCGa                       >  2:15358/1‑149 (MQ=255)
                                                                                                                        gACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATGCCTGGCTGTGCAGTAGTCGAGGCCGAGATCGATGGCGTACTCCATGAGTACTCCGCGa                       <  6:155357/149‑1 (MQ=255)
                                                                                                                        gACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATGCCTGGCTGTGCAGTAGTCGAGGCCGAGATCGATGGCGTACTCCATGAGTACTCCGCGa                       <  6:321717/149‑1 (MQ=255)
                                                                                                                             gcgcCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATGCCTGGCTGTGCAGTAGTCGAGGCCGAGATCGATGGCGTACTCCATGAGTACTCCGCGATCg                    >  3:41702/1‑147 (MQ=255)
                                                                                                                             gcgcCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATGCCTGGCTGTGCAGTAGTCGAGGCCGAGATCGATGGCGTACTCCATGAGTACTCCGCGATCGaa                  <  3:297849/149‑1 (MQ=255)
                                                                                                                             gcgcCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATGCCTGGCTGTGCAGTAGTCGAGGCCGAGATCGATGGCGTACTCCATGAGTACTCCGCGATCGaa                  <  3:91485/149‑1 (MQ=255)
                                                                                                                             gcgcCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATGCCTGGCTGTGCAGTAGTCGAGGCCGAGATCGATGGCGTACTCCATGAGTACTCCGCGATCGaa                  <  8:305607/149‑1 (MQ=255)
                                                                                                                             gcgcCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATGCCTGGCTGTGCAGTAGTCGAGGCCGAGATCGATGGCGTACTCCATGAGTACTCCGCGATCGaa                  <  6:5863/149‑1 (MQ=255)
                                                                                                                             gcgcCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATGCCTGGCTGTGCAGTAGTCGAGGCCGAGATCGATGCCGTACTCCATGAGTACTCCGCGATCGaa                  <  1:204567/149‑1 (MQ=255)
                                                                                                                              cgcCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATGCCTGGCTGTGCAGTAGTCGAGGCCGAGATCGATGGCGTACTCCATGAGTACTCCGCGATCGAAg                 <  4:41702/149‑1 (MQ=255)
                                                                                                                                ccAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATGCCTGGCTGTGCAGTAGTCGAGGCCGAGATCGATGGCGTACTCCATGAGTACTCCGCGATCGAAGgt               <  1:351636/149‑1 (MQ=255)
                                                                                                                                 cAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATGCCTGGCTGTGCAGTAGTCGAGGCCGAGATCGAt                                                >  1:57843/1‑115 (MQ=255)
                                                                                                                                 cAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATGCCTGGCTGTGCAGTAGTCGAGGCCGAGATCGAt                                                <  2:57843/115‑1 (MQ=255)
                                                                                                                                 cAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATGCCTGGCTGTGCAGTAGTCGAGGCCGAGATCGATGGCGTACTCCTTGAGTACTCCGCGATCGAAGgtg              <  7:244592/149‑1 (MQ=255)
                                                                                                                                    aaTTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATGCCTGGCTGTGCAGTAGTCGAGGCCGAGATCGATGGCGTACTCCATGAGTACTCCGCGATCGAAGGTGTTc           <  1:320261/149‑1 (MQ=255)
                                                                                                                                       tACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATGCCTGGCTGTGCAGTAGTCGAGGCCGAGATCGATGGCGTACTCCATGa                                  <  3:105941/123‑1 (MQ=255)
                                                                                                                                       tACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATGCCTGGCTGTGCAGTAGTCGAGGCCGAGATCGATGGCGTACTCCATGa                                  >  4:105941/1‑123 (MQ=255)
                                                                                                                                         cGCTCGAGAGGCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATGCCTGGCTGTGCAGTAGTCGAGGCCGAGATCGATGGCGTACTCCATGAGTACTCCGCGATCGAAGGTGTTCAgg        >  7:262644/1‑147 (MQ=255)
                                                                                                                                          gCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATGCCTGGCTGTGCAGTAGTCGAGGCCGAGATCGATGGCGTACTCCATGAGTACTCAGCGATCGAAGGTGTTCAgg        >  4:130398/1‑146 (MQ=255)
                                                                                                                                            tCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATGCCTGGCTGTGCAGTAGTCGAGGCCGAGATCGATGGCGTACTCCATGAGTACTCCGCGATCGAAGGTGTTCAGGAAGAc   >  1:167498/1‑149 (MQ=255)
                                                                                                                                              gAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATGCCTGGCTGTGCAGTAGTCGAGGCCGAGATCGATGGCGTACTCCATGAGTACTCCGCGATCGAAGGTGTTCAGGAAGACg  <  5:10624/148‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                              
AACGAAAACCTGATTGTCGAGCTCTACTCCAAGTAAGGGCTAGAAAATAGGTGCATCCATGCAGATTTCGGTAAATGAGTTCCTGACTCCCCGCCACATTGATGTGCAGGTAGTCAGTCCGACCCGCGCCAAAATTACGCTCGAGCCTCTCGAGCGTGGTTTCGGCCATACCCTGGGCAACGCGCTGCGCCGCATCCTGTTGTCCTCCATGCCTGGCTGTGCAGTAGTCGAGGCCGAGATCGATGGCGTACTCCATGAGTACTCCGCGATCGAAGGTGTTCAGGAAGACG  >  NC_002947/562860‑563149

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑