Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 572,518 A→G 9.1% I191T (ATC→ACC)  PP_0486 ← GntR family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947572,5180AG 9.1% 146.6 / 5.4 55I191T (ATC→ACC) PP_0486GntR family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base A (26/24);  new base G (2/3);  total (28/27)
Fisher's exact test for biased strand distribution p-value = 6.69e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.44e-01

CGCAATGCCTCCGGGATGAGCCCGTGTTCGTCCATGGCGACGCCGCGCAGGCGCCGGCCCAGGCTGTGGGCCAGGGATATGATGCCCGGGTAGCAGAGCGACTCGCACAGCACCACATCGTCACTCTTCGACAGGCTGCTGATGGCCACCAGCAGCCCATGTTGGGCGCCAGCGGTCAGTACTACCTGCTGCCAGCGAGCATCAGGCAGCCAGCGCTGTATCCAGGTCGCACCGGCTTGTTTGTGGGACGTGTGACCACCCTCTGTCACATAGTCGAG  >  NC_002947/572377‑572654
                                                                                                                                             |                                                                                                                                        
cgcAATGCCTCCGGGATGAGCCCGTGTTCGTCCATGGCGACGCCGCGCAGGCGCCGGCCCAGGCTGTGGGCCAGGGATATGATGCCCGGGTAGCAGAGCGACTCGCACAGCACCACATCGTCACTCTTCGACAGGCTGCTGATGGccac                                                                                                                                   <  8:296966/149‑1 (MQ=255)
 gcAATGCCTCCGGGATGAGCCCGTGTTCGTCCATGGCGACGCCGCGCAGGCGCCGGCCCAGGCTGTGGGCCAGGGATATGATGCCCGGGTAGCAGAGCGACTCGCACAGCACCACATCGTCACTCTTCGACAGGCTGCTGATg                                                                                                                                        >  4:238057/1‑143 (MQ=255)
 gcAATGCCTCCGGGATGAGCCCGTGTTCGTCCATGGCGACGCCGCGCAGGCGCCGGCCCAGGCTGTGGGCCAGGGATATGATGCCCGGGTAGCAGAGCGACTCGCACAGCACCACATCGTCACTCTTCGACAGGCTGCTGATg                                                                                                                                        <  3:238057/143‑1 (MQ=255)
   aaTGCCTCCGGGATGAGCCCGTGTTCGTCCATGGCGACGCCGCGCAGGCGCCGGCCCAGGCTGTGGGCCAGGGATATGATGCCCGGGTAGCAGAGCGACTCGCACAGCACCACATCGTCACTCTTCGACAGGCTGCTGGTGGCCATcag                                                                                                                                >  6:46846/1‑149 (MQ=255)
     tGCCTCCGGGATGAGCCCGTGTTCGTCCATGGCGACGCCGCGCAGGCGCCGGCCCAGGCTGTGGGCCAGGGATATGATGCCCGGGTAGCAGAGCGACTCGCACAGCACCACATCGTCACTCTTCGACAGGCTGCTGGTGGCCATcagca                                                                                                                              <  2:27120/149‑1 (MQ=255)
         tCCGGGATGAGCCCGTGTTCGTCCATGGCGACGCCGCGCAGGCGCCGGCCCAGGCTGTGGGCCAGGGATATGATGCCCGGGTAGCAGAGCGACTCGCACAGCACCACATCGTCACTCTTCGACAGGCTGCTGATGGCCACCAGCAGcc                                                                                                                           >  8:326511/1‑148 (MQ=255)
         tCCGGGATGAGCCCGTGTTAGTCCATGGCGACGCCCCGCAGGCGCCGGCCCAGGCTGTGGGCCTGGGATATGAGGCGCGGGTAGCAGAGCGACTCGCACAGCACCACATCGTCACTCTGCGACAGGCTGATGATCGCCACCCGCAGcc                                                                                                                           >  8:271689/1‑148 (MQ=255)
           cGGGATGAGCCCGTGTTCGTCCATGGCGACGCCGCGCAGGCGCCGGCCCAGGCTGTGGGCCAGGGATATGATGCCCGGGTAGCAGAGCGACTCGCACAGCACCACATCGTCACTCTTCGACAGGCTGCTGGTGGCCATCAGCAGcctg                                                                                                                         <  4:293020/148‑3 (MQ=255)
             ggATGAGCCCGTGTTCGTCCATGGCGACGCCGCGCAGGCGCCGGCCCAGGCTGTGGGCCAGGGATATGATGCCCGGGTAGCAGAGCGACTCGCACAGCACCACATCGTCACTCTTCGACAGGCTGCTGATGGCCACCAGCAGCCCATGt                                                                                                                      >  5:355882/1‑149 (MQ=255)
              gATGAGCCCGTGTTCGTCCATGGCGACGCCGCGCAGGCGCCGGCCCAGGCTGTGGGCCAGGGATATGATGCCCGGGTAGCAGAGCGACTCGCACAGCACCACATCGTCACTCTTCGACAGGCTGCTGATGGCCACCAGCAGCCCATGtt                                                                                                                     >  1:223153/1‑149 (MQ=255)
               aTGAGCCCGTGTTCGTCCATGGCGACGCCGCGCAGGCGCCGGCCCAGGCTGTGGGCCAGGGATATGATGCCCGGGTAGCAGAGCGACTCGCACAGCACCACATCGTCACTCTTCGACAGGCTGCTGGTGGCCATCAGCAGcctgtcgaa                                                                                                                    <  5:46846/149‑8 (MQ=255)
                   gCCCGTGTTCGTCCATGGCGACGCCGCGCAGGCGCCGGCCCAGGCTGTGGGCCAGGGATATGATGCCCGGGTAGCAGAGCGACTCGCACAGCACCACATCGTCACTCTTCGACAGGCTGCTGATGGCCACCAGCAGCCCATGTTGGgcg                                                                                                                >  7:177485/1‑149 (MQ=255)
                    cccGTGTTCGTCCATGGCGACGCCGCGCAGGCGCCGGCCCAGGCTGTGGGCCAGGGATATGATGCCCGGGTAGCAGAGCGACTCGCACAGCACCACATCGTCACTCTTCGACAGGCTGCTGATGGCCACCAGCAGCCCATGTTGGgcgc                                                                                                               >  5:211615/1‑149 (MQ=255)
                       gtgtTCGTCCATGGCGACGCCGCGCAGGCGCCGGCCCAGGCTGTGGGCCAGGGATATGATGCCCGGGTAGCAGAGCGACTCGCACAGCACCACATCGTCACTCTTCGACAGGCTGCTGATGGCCACCAGCAGCCCATGTTGGGCGCCAg                                                                                                            >  4:191729/1‑149 (MQ=255)
                             gTCCATGGCGACGCCGCGCAGGCGCCGGCCCAGGCTGTGGGCCAGGGATATGATGCCCGGGTAGCAGAGCGACTCGCACAGCACCACATCGTCACTCTTCGACAGGCTGCTGATGGCCACCAGCAGCCCATGTTGGGCGCCAGCGGt                                                                                                        >  3:241900/1‑147 (MQ=255)
                                 aTGGCGACGCCGCGCAGGCGCCGGCCCAGGCTGTGGGCCAGGGATATGATGCCCGGGTAGCAGAGCGACTCGCACAGCACCACATCGTCACTCTTCGACAGGCTGCTGATGGCCACCAGCAGCCCATGTTGGGCGCCAGCGGTCAGta                                                                                                   >  4:67081/1‑148 (MQ=255)
                                    gCGACGCCGCGCAGGCGCCGGCCCAGGCTGTGGGCCAGGGATATGATGCCCGGGTAGCAGAGCGACTCGCACAGCACCACATCGTCACTCTTCGACAGGCTGCTGATGGCCACCAGCAGCCCATGTTGGGCGCCAGCGGTCAGtactac                                                                                               <  5:279602/149‑1 (MQ=255)
                                       aCGCCGCGCAGGCGCCGGCCCAGGCTGTGGGCCAGGGATATGATGCCCGGGTAGCAGAGCGACTCGCACAGCACCACATCGTCACTCTTCGACAGGCTGCTGATGGCCACCAGCAGCCCATGTTGGGCGCCAGCGGTCAGTACTACctg                                                                                            >  2:8182/1‑149 (MQ=255)
                                       aCGCCGCGCAGGCGCCGGCCCAGGCTGTGGGCCAGGGATATGATGCCCGGGTAGCAGAGCGACTCGCACAGCACCACATCGTCACTCTTCGACAGGCTGCTGATGGCCACCAGCAGCCCATGTTGGGCGCCAGCGGTCAGTACTACctg                                                                                            <  3:67081/149‑1 (MQ=255)
                                         gccgcGCAGGCGCCGGCCCAGGCTGTGGGCCAGGGATATGATGCCCGGGTAGCAGAGCGACTCGCACAGCACCACATCGTCACTCTTCGACAGGCTGCTGATGGCCACCAGCAGCCCATGTTGGGCGCCAGCGGTCAGTACTACctgct                                                                                          <  2:223153/149‑1 (MQ=255)
                                          ccgcGCAGGCGCCGGCCCAGGCTGTGGGCCAGGGATATGATGCCCGGGTAGCAGAGCGACTCGCACAGCACCACATCGTCACTCTTCGACAGGCTGCTGATGGCCACCAGCAGCCCATGTTGGGCGCCAGCGGTCAGTACTACctgctg                                                                                         <  7:326511/149‑1 (MQ=255)
                                           cgcgCAGGCGCCGGCCCAGGCTGGGCGCCAGCGATATGAAGCCCGGGTAGCAGAGCGACTCGCACACCACCACATCGTCACTCTTCGACAGGCTGCTGATGGCCACCAGCAGCCCATGTTGGGCGCCAGCGGTCAGTACTACCtgctgc                                                                                        <  8:89965/149‑1 (MQ=255)
                                                    gcCGGCCCAGGCTGTGGGCCAGGGATATGATGCCCGGGTAGCAGAGCGACTCGCACAGCACCACATCGTCACTCTTCGACAGGCTGCTGATGGCCACCAGCAGCCCATGTTGGGCGCCACCGGTCAGTACTACCTGCTGCCCGCGAGCa                                                                               >  3:334842/1‑149 (MQ=255)
                                                    gcCGGCCCAGGCTGTGGGCCAGGGATATGATGCCCGGGTAGCAGAGCGACTCGCACAGCACCACATCGTCACTCTTCGACAGGCTACTGATGGCCACCAGCAGCCCATGTTGGGCGCCAGCGGTCAGTACTACCTGCTGCCAGCGAGCa                                                                               >  3:95204/1‑149 (MQ=255)
                                                     ccGGCCCAGGCTGTGGGCCAGGGATATGATGCCCGGGTAGCAGAGCGACTCGCACAGCACCACATCGTCACTCTTCGACAGGCTGCTGATGGCCACCAGCAGCCCATGTTGGGCGCCAGCGGTCAGTACTACCTGCTGCCAGCGAGCAt                                                                              <  6:355882/149‑1 (MQ=255)
                                                          ccAGGCTGTGGGCCAGGGATATGATGCCCGGGTAGCAGAGCGACTCGCACAGCACCACATCGTCACTCTTCGACAGGCTGCTGATGGCCACCAGCAGCCCATGTTGGGCGcc                                                                                                              >  6:335902/1‑112 (MQ=255)
                                                          ccAGGCTGTGGGCCAGGGATATGATGCCCGGGTAGCAGAGCGACTCGCACAGCACCACATCGTCACTCTTCGACAGGCTGCTGATGGCCACCAGCAGCCCATGTTGGGCGcc                                                                                                              <  5:335902/112‑1 (MQ=255)
                                                                  tgGGCCAGGGATATGATGCCCGGGTAGCAGAGCGACTCGCACAGCACCACATCGTCACTCTTCGACAGGCTGCTGATGGCCACCAGCAGCCCATGTTGGGCGCCAGCGGTCAGTACTACCTGCTGCCAGCGAGCATCAGGCAGCCAgcg                                                                 <  7:87091/149‑1 (MQ=255)
                                                                          ggATATGATGCCCGGGTAGCAGAGCGACTCGCACAGCACCACATCGTCACTCTTCGACAGGCTGCTGATGGCCACCAGCAGCCCATGTTGGGCGCCAGCGGTCAGTACTACCTGCTGCCAGCGAGCATCAGGCAGCCAGCGCTGTATcc                                                         <  4:241900/149‑1 (MQ=255)
                                                                          ggATATGATGCCCGGGTAGCAGAGCGACTCGCACAGCACCACATCGTCACTCTTCGACAGGCTGCTGATGGCCACCAGCAGCCCATGTTGGGCGCCAGCGGTCAGTACTACCTGCTGCCAGCGAGCATCAGGCAGCCAGCGCTGTATcc                                                         <  6:211615/149‑1 (MQ=255)
                                                                              atgatgCCCGGGTAGCAGAGCGACTCGCACAGCACCACATCGTCACTCTTCGACAGGCTGCTGATGGCCACCAGCAGCCCATGTTGGGCGCCAGCGGTCAGTACTACCTGCTGCCAGCGAGCATCAGGCAGCCAgcgc                                                                <  8:293009/138‑1 (MQ=255)
                                                                              atgatgCCCGGGTAGCAGAGCGACTCGCACAGCACCACATCGTCACTCTTCGACAGGCTGCTGATGGCCACCAGCAGCCCATGTTGGGCGCCAGCGGTCAGTACTACCTGCTGCCAGCGAGCATCAGGCAGCCAgcgc                                                                >  7:293009/1‑138 (MQ=255)
                                                                               tgatgCCCGGGTAGCAGAGCGACTCGCACAGCACCACATCGTCACTCTTCGACAGGCTGCTGATGGCCACCAGCAGCCCATGTTGGGCGCCAGCGGTCAGTACTACCTGCTGCCAGCGAGCATCAGGCAGCCAGCGCTGTATCCAGGTc                                                    >  1:165908/1‑149 (MQ=255)
                                                                                              agagCGACTCGCACAGCACCACATCGTCACTCTTCGACAGGCTGCTGATGGCCACCAGCAGCCCATGTTGGGCGCCAGCGGTCAGTACTACCTGCTGCCAGCGAGCATCAGGCAGCCAGCGCTGTATCCAGGTCGCACCGGCttgtttg                                     >  4:281400/1‑149 (MQ=255)
                                                                                               gagCGACTCGCACAGCACCAGATCTTCACTATTCGACAGGATTCTGATGGCCACCAGCAGCCCATTTTGGGCGCAAGCGGTCAGTACTACCTGCTTCCAGCGAGCATCAGGCAGCCAGCTCTGTATCCAGGTCGCACCGGCttgtttgt                                    <  4:334842/149‑1 (MQ=255)
                                                                                               gagCGACTCGCACAGCACCACATCGTCACTCTTCGACAGGCTGCTGATGGCCACCAGCAGCCCATGTTGGGCGCCAGCGGTCAGTACTACCTGCTGCCAGCGAGCATCAGGCAGCCAGCGCTGTATCCAGGTCGCACCGGCttgtttgt                                    >  6:106999/1‑149 (MQ=255)
                                                                                               gagCGACTCGCACAGCACCACATCGTCACTCTTCGACAGGCTGCTGATGGCCACCAGCAGCCCATGTTGGGCGCCAGCGGTCAGTACTACCTGCTGCCAGCGAGCATCAGGCAGCCAGCGCTGTATCCAGGTCGCACCGGCttgtttgt                                    >  5:259658/1‑149 (MQ=255)
                                                                                                agCGACTCGCACAGCACCACATCGTCACTCTTCGACAGGCTGCTGATGGCCACCAGCAGCCCATGTTGGGCGCCAGCGGTCAGTACTACCTGCTGCCAGCGAGCATCAGGCAGCCAGCGCTGTATCCAGGTCGCACCGGCTTGTTtgtg                                   <  6:17929/149‑1 (MQ=255)
                                                                                                agCGACTCGCACAGCACCACATCGTCACTCTTCGACAGGCTGCTGATGGCCACCAGCAGCCCATGTTGGGCGCCAGCGGTCAGTACTACCTGCTGCCAGCGAGCATCAGGCAGCCAGCGCTGTATCCAGGTCGCACCGGCTTGTTtgtg                                   <  8:177485/149‑1 (MQ=255)
                                                                                                agCGACTCGCACAGCACCACATCGTCACTCTTCGACAGGCTACTGATGGCCACCAGCAGCCCATGTTGGGCGCCAGCGGTCAGTACTACCTGCTGCCAGCGAGCATCAGGCAGCCAGCGCTGTATCCAGGTCGCACCGGCTTGTTtgtg                                   <  4:95204/149‑1 (MQ=255)
                                                                                                   gACTCGCACAGCACCACATCGTCACTCTTCGACAGGCTGCTGATGGCCACCAGCAGCCCATGTTGGGCGCCAGCGGTCAGTACTACCTGCTGCCAGCGAGCATCAGGCAGCCAGCGCTGTATCCAGGTCGCACCGGCTTGTTTGTGGGa                                <  6:136885/149‑1 (MQ=255)
                                                                                                        gCACAGCACCACATCGTCACTCTTCGGCAGGCTGCTGATGGCCACCAGCAGCCCATGTTGGGCGCCAGCGGTCAGTACTACCTGCTGCCAGCGAGCATCAGGCAGCCAGCGCTGTATCCAGGTCGCACCGGCTTGTTTGTGGGACgtg                            >  1:235198/1‑148 (MQ=255)
                                                                                                        gCACAGCACCACATCGTCACTCTTCGCCAGGCTGCTGATGGCCACCAGCAGCCCATGTTGGGCGCCAGCGGTCAGTACTACCTGCTGCCAGCGAGCATCAGGCAGCCAGCGCTGTATCCAGGTCGCACCGGCTTGTTTGTGGGACgtg                            <  2:235198/148‑1 (MQ=255)
                                                                                                        gCACAGCACCACATCGTCACTCTTCGACAGGCTGCTGATGGCCACCAGCAGCCCATGTTGGGCGCCAGCGGTCAGTACTACCTGCTGCCAGCGAg                                                                                 <  4:198218/95‑1 (MQ=255)
                                                                                                        gCACAGCACCACATCGTCACTCTTCGACAGGCTGCTGATGGCCACCAGCAGCCCATGTTGGGCGCCAGCGGTCAGTACTACCTGCTGCCAGCGAg                                                                                 >  3:198218/1‑95 (MQ=255)
                                                                                                            aGCACCACATCGTCACTCTTCGACAGGCTGCTGATGGCCACCAGCAGCCCATGTTGGGCGCCAGCGGTCAGTACTACCTGCTGCCAGCGAGCATCAGGCAGCCAGCGCTGTATCCAGGTCGCACCGGCTTGTTTGTGGGACGTGTGac                        >  2:258346/1‑148 (MQ=255)
                                                                                                                ccacATCGTCACTCTTCGACAGGCTGCTGATGGCCACCAGCAGCCCATGTTGGGCGCCAGCGGTCAGTACTACCTGCTGCCAGCGAGCATCAGGCAGCCAGCGCTGTATCCAGGTCGCACCGGCTTGTTTGTGGGACGTGTGACCAccc                   >  8:329625/1‑149 (MQ=255)
                                                                                                                 cacaTCGTCACTCTTCGACAGGCTGCTGATGGCCACCAGCAGCCCATGTTCGGCGCCAGCGGTCAGTACTACCTGCTGCAAGCGAGCATCAGGCAGCCAGCGCTGTATCCAGGTAGCACCGGCTTGTTTGTGGCACATGTGACCACCct                  >  7:268019/1‑149 (MQ=255)
                                                                                                                   caTCGTCACTCTTCGACAGGCTGCTGATGGCCACCAGCAGCCCATGTTGGGCGCCAGCGGTCAGTACTACCTGCTGCCAGCGAGCATCAGGCAGCCAGCGCTGTATCCAGGTCGCACCGGCTTGTTTGTGGGACGTGTGACCACCctct                >  3:254850/1‑149 (MQ=255)
                                                                                                                   caTCGTCACTCTTCGACAGGCTGCTGATGGCCACCAGCAGCCCATGTTGGGCGCCAGCGGTCAGTACTACCTGCTGCCAGCGAGCATCAGGCAGCCAGCGCTGTATCCAGGTCGCACCGGCTTGTTTGTGGGACGTGTGACCACCctct                <  3:191729/149‑1 (MQ=255)
                                                                                                                   caTCGTCACTCTTCGACAGGCTGCTGATGGCCACCAGCAGCCCATGTTGGGCGCCAGCGGTCAGTACTACCTGCTGCCAGCGAGCATCAGGCAGCCAGCGCTGTATCCAGGTCGCACCGGCTTGTTTGTGGGACGTGTGACCACCctct                <  8:186462/149‑1 (MQ=255)
                                                                                                                   caTCGTCACTCTTCGACAGGCTGCTGATGGCCACCAGCAGCCCATGTTGGGCGCCAGCGGTCAGTACTACCTGCTGCCAGCGAGCATCAGGCAGCCAGCGCTGTATCCAGGTCGCACCGGCTTGTTTGTGGGACGTGTGACCACCctct                <  2:165908/149‑1 (MQ=255)
                                                                                                                            tctTCGACAGGCTGCTGATGGCCACCAGCAGCCCATGTTGGGCGCCAGCGGTCAGTACTACCTGCTGCCAGCGAGCATCAGGCAGCCAGCGCTGTATCCAGGTCGCACCGGCttgtttgt                                    <  4:202224/120‑1 (MQ=255)
                                                                                                                            tctTCGACAGGCTGCTGATGGCCACCAGCAGCCCATGTTGGGCGCCAGCGGTCAGTACTACCTGCTGCCAGCGAGCATCAGGCAGCCAGCGCTGTATCCAGGTCGCACCGGCttgtttgt                                    >  3:202224/1‑120 (MQ=255)
                                                                                                                                 catgggCTGCTGGTGGCCATCAGCAGCCCATGTTGGGCGCCAGCGGTCAGTACTACCTGCTGCCAGCGAGCATCAGGCAGCCAGCGCTGTATCCAGGTCGCACCGGCTTGTTTGTGGGACGTGTGACCACCCTCTGTCACATAGTCGAg  >  5:2249/5‑149 (MQ=255)
                                                                                                                                             |                                                                                                                                        
CGCAATGCCTCCGGGATGAGCCCGTGTTCGTCCATGGCGACGCCGCGCAGGCGCCGGCCCAGGCTGTGGGCCAGGGATATGATGCCCGGGTAGCAGAGCGACTCGCACAGCACCACATCGTCACTCTTCGACAGGCTGCTGATGGCCACCAGCAGCCCATGTTGGGCGCCAGCGGTCAGTACTACCTGCTGCCAGCGAGCATCAGGCAGCCAGCGCTGTATCCAGGTCGCACCGGCTTGTTTGTGGGACGTGTGACCACCCTCTGTCACATAGTCGAG  >  NC_002947/572377‑572654

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑