Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	593,434	A→C	9.1%	E391A (GAG→GCG) ‡	PP_0503 →	MFS transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	593,434	0	A	C	9.1%	178.5 / 7.3	64	E391A (GAG→GCG) ‡	PP_0503	MFS transporter
Reads supporting (aligned to +/- strand):  ref base A (32/26);  new base C (4/2);  total (36/28)
Fisher's exact test for biased strand distribution p-value = 6.88e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CAGCGGTTTGGGGGCGGTGTTGGCGAGCTGGGTGTTTGCACACTGGGGCTGGGTTGCGGTGTGTGGGCTTGGCATGGGCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGA‑GGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCACGGGTCGACGTCGACGTCGACCTTGGTCTTGCTGTAGCCAAGGGCGGTCGGGCCGTTGACGCTGGCCTTGGTGTCGATGTCG  >  NC_002947/593300‑593573                                                                                                                                        |                                                                                                                                            cAGCGGTTTGGGGGTGGTGTTGGCGAGCTGGGTGTTTGCACACTGGGGCTGGGTTGCGGTGTGTGGGCTTGGCATGGGCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGA‑GGGCGGCCGAGGCCGCCCTGCAAt                                                                                                                               >  2:248669/1‑149 (MQ=255)      gTTTGGGGGCGGTGTTGGCGAGCTGGGTGTTTGCACACTGGGGCTGGGTTGCGGTGTGTGGGCTTGGCATGGGCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGA‑GGGCGGCCGAGGCCGCCCTGCAATGTTCa                                                                                                                          <  5:138150/149‑1 (MQ=255)       tttGGGGGCGGTGTTGGCGAGCTGGGTGTTTGCACACTGGGGCTGGGTTGCGGTGTGTGGGCTTGGCATGGGCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGA‑GGGCGGCCGAGGCCGCCCTGCAATGTTCAg                                                                                                                         >  7:15296/1‑149 (MQ=255)       tttGGGGGCGGTGTTGGCGAGCTGGGTGTTTGCACACTGGGGCTGGGTTGCGGTGTGTGGGCTTGGCATGGGCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGA‑GGGCGGCCGAGGCCGCCCTGCAATGTTCAg                                                                                                                         <  5:284256/149‑1 (MQ=255)           ggggCGGTGTTGGCGAGCTGGGTGTTTGCACACTGGGGCTGGGTTGCGGTGTGTGGGCTTGGCATGGGCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGA‑GGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTc                                                                                                                      <  3:94878/148‑1 (MQ=255)             ggCGGTGTTGGCGAGCTGGGTGTTTGCACACTGGGGCTGGGTTGCGGTGTGTGGGCTTGGCATGGGCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGA‑GGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAg                                                                                                                    >  6:143412/1‑148 (MQ=255)              gCGGTGTTGGCGAGCTGGGTGTTTGCACACTGGTGCTGGGTTGCGGTGTGTGGGCTTGGCATGGGCATCAGTGCGGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGAGGGGCGGCCTAGGCCGCCCTGCAATGTTCAg                                                                                                                         <  1:205876/143‑1 (MQ=255)              gCGGTGTTGGCGAGCTGGGTGTTTGCACACTGGGGCTGGGTTGCGGTGTGTGGGCTTCGCATGGGCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGA‑GGGCGGCCGCGGCCGCCCTGCAATGTTCAg                                                                                                                         >  2:205876/1‑142 (MQ=255)                    ttGGCGAGCTGGGTGTTTGCACACTGGGGCTGGGTTGCGGTGTGTGGGCTTGGCATGGGCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGA‑GGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTg                                                                                                              >  4:235221/1‑147 (MQ=255)                    ttGGCGAGCTGGGTGTTTGCACACTGGGGCTGGGTTGCGGTGTGTGGGCTTGGCATGGGCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGA‑GGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTa                                                                                                            <  3:112174/149‑1 (MQ=255)                            cTGGGTGTTTGCACACTGGGGCTGGGTTGCGGTGTGTGGGCTTGGCATGGGCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGA‑GGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAgac                                                                                                    <  1:248669/149‑1 (MQ=255)                               ggTGTTTGCACACTGGGGCTGGGTTGCGGTGTGTGGGCTTGGCATGGGCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGA‑GGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAgaccga                                                                                                 <  5:143412/149‑1 (MQ=255)                                  gtTTGCACACTGGGGCTGGGTTGCGGTGTGTGGGCTTGGCATGGGCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGA‑GGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCa                                                                                              >  8:346910/1‑149 (MQ=255)                                      gCACACTGGGGCTGGGTTGCGGTGTGTGGGCTTGGCATGGGCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGA‑GGGCGGCCGAGGCCGCCCTGCAATGtt                                                                                                                            <  2:316951/115‑1 (MQ=255)                                      gCACACTGGGGCTGGGTTGCGGTGTGTGGGCTTGGCATGGGCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGA‑GGGCGGCCGAGGCCGCCCTGCAATGtt                                                                                                                            >  1:316951/1‑115 (MQ=255)                                      gCACACTGGGGCTGGGTTGCGGTGTGTGGGCTTGGCATGGGCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGA‑GGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAg                                                                                                           <  8:249876/132‑1 (MQ=255)                                      gCACACTGGGGCTGGGTTGCGGTGTGTGGGCTTGGCATGGGCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGA‑GGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAg                                                                                                           >  7:249876/1‑132 (MQ=255)                                      gCACACTGGGGCTGGGTTGCGGTGTGTGGGCTTGGCATGGGCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGA‑GGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAg                                                                                                           <  6:30893/132‑1 (MQ=255)                                      gCACACTGGGGCTGGGTTGCGGTGTGTGGGCTTGGCATGGGCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGA‑GGGAGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAg                                                                                                           >  5:30893/1‑132 (MQ=255)                                        acacTGGGGCTGGGTTGCGGTGTGTGGGCTTGGCATGGGCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGA‑GGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGtata                                                                                        >  3:268570/1‑149 (MQ=255)                                                         cGGTGTGTGGGCTTGGCATGGGCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGA‑GGGCGGCCGAGGCCGCCCTg                                                                                                                                   >  6:272300/1‑89 (MQ=255)                                                         cGGTGTGTGGGCTTGGCATGGGCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGA‑GGGCGGCCGAGGCCGCCCTg                                                                                                                                   <  5:272300/89‑1 (MQ=255)                                                           gtgtgtGGGCTTGGCATGGGCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGA‑GGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCACGGgtcgacgtcgac                                                                     >  3:149549/1‑149 (MQ=255)                                                              tgtgGGCTTGGCATGGGCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGA‑GGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCACGGgtcgacgtcgacgt                                                                   <  2:214005/148‑1 (MQ=255)                                                              tgtgGGCTTGGCATGGGCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGA‑GGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCACGGgtcgacgtcgacgt                                                                   >  1:214005/1‑148 (MQ=255)                                                               gtgGGCTTGGCATGGGCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGA‑GGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCACGGgtcgacgtcgacgtc                                                                  >  7:301583/1‑148 (MQ=255)                                                                       ggCATGGGCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGA‑GGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCACGGGTCGACGTCGACGTCGACCTTg                                                           <  3:235221/147‑1 (MQ=255)                                                                       ggCATGGGCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGA‑GGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCACGGGTCGACGTCGACGTCGACCTTGGt                                                         >  7:321563/1‑149 (MQ=255)                                                                               gcacAGTGCGGCGGCACTGTGCTACTGGCTATGGCTGCAACGCGCGA‑GGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCACGGGTCGACGTCGACGTCGACCTTGGTCTTGCTGt                                                 >  5:323580/4‑149 (MQ=255)                                                                               cATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGA‑GGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCACGGGTCGACGTCGACGTCGACCTTGGTCTTGCTg                                                  >  2:44530/1‑148 (MQ=255)                                                                                   aGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGA‑GGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTa                                                                                                            <  7:190632/86‑1 (MQ=255)                                                                                   aGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGA‑GGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTa                                                                                                            >  8:190632/1‑86 (MQ=255)                                                                                    gTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGA‑GGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCACGGgtcgac                                                                           >  3:216413/1‑118 (MQ=255)                                                                                    gTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGA‑GGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCACGGgtcgac                                                                           <  4:216413/118‑1 (MQ=255)                                                                                     tGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGA‑GGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCACGGGTCGACGTCGACGTCGACCTTGGTCTTGCTGTAGCCaa                                           <  7:346910/149‑1 (MQ=255)                                                                                      gccgccGCACTGTGCTACTGGCTATGGCTGCAACGCGCGA‑GGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCACGGGTCGACGTCGACGTCGACCTTGGTCTTGCTGTAGCCAAg                                          >  3:260970/1‑149 (MQ=255)                                                                                      gccgccGCACTGTGCTACTGGCTATGGCTGCAACGCGCGA‑GGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCACGGGTCGACGTCGACGTCGACCTTGGTCTTGCTGTAGCCAAg                                          >  5:139394/1‑149 (MQ=255)                                                                                             cACTGTGCTACTGGCTATGGCTGCAACGCGCGA‑GGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCACGGGTCGACGTCGACGTCGACCTTGGTCTTGCTGTAGCCAAGGGCGGTc                                   <  4:268570/149‑1 (MQ=255)                                                                                              aCTGTGCTACTGGCTATGGCTGCAACGCGCGA‑GGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCACGGGTCGACGTCGACGTCGACCTTGGTCTTGCTGTAGCCAAGGGCGGTCg                                  >  2:64832/1‑149 (MQ=255)                                                                                                  tgCTACTGGATTTGGCTGCAAGGCGCGA‑GGGCGGCCGAGGCCTCCCGGCAATGTTCAGGTCAGATCTTGTAGCCAAGACCGACCATGTATACCCACGGGTCGACGTCGACGTCGACCTTGGTCTTGCTGTAGCCAAGGGCGGTCggg                                <  8:15296/147‑1 (MQ=255)                                                                                                    cTACTGGCTATGGCTGCAACGCGCGA‑GGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCACGGGTCGACGTCGACGTCGACCTTGGTCTTGCTGTAGCCAAGGGCGGTCGGGCCg                             <  8:321563/148‑1 (MQ=255)                                                                                                     tACTGGCTATGGCTGCAACGCGCGA‑GGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCACGGGTCGACGTCGACGTCGACCTTGGTCTTGCTGTAGCCAAGGGCGGTCGGGCCGtt                           >  3:220932/1‑149 (MQ=255)                                                                                                          gCTATGGCTGCAACGCGCGA‑GGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCACGGGTCGACGTCGACGTCGACCTTGGTCTTGCTGTAGCCAAGGGCGGTCGGGCCGTTGACGc                      >  2:8342/1‑149 (MQ=255)                                                                                                           cTATGGCTGCAACGCGCGA‑GGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCACGGGTCGACGTCGACGTCGACCTTGGTCTTGCTGTAGCCAAGGGCGGTCGGGCCGTTGACGCt                     <  6:323580/149‑1 (MQ=255)                                                                                                           cTATGGCTGCAACGCGCGA‑GGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCACGGGTCGACGTCGACGTCGACCTTGGTCTTGCTGTAGCCAAGGGCGGTCGGGCCGTTGACGCt                     >  3:145271/1‑149 (MQ=255)                                                                                                            tATGGCTGCAACGCGCGA‑GGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCACGGgtc                                                                              >  1:177340/1‑91 (MQ=255)                                                                                                            tATGGCTGCAACGCGCGA‑GGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCACGGgtc                                                                              <  2:177340/91‑1 (MQ=255)                                                                                                            tATGGCTGCAACGCGCGA‑GGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCACGGGTCGACGTCGACGTCGACCTTGGTCTTGCTGTAGCCAAGGGCGGTCGGGCCGTTGACGCTg                    <  4:149549/149‑1 (MQ=255)                                                                                                            tATGGCTGCAACGCGCGA‑GGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCACGGGTCGACGTCGACGTCGACCTTGGTCTTGCTGTAGCCAAGGGCGGTCGGGCCGTTGACGCTg                    >  3:76200/1‑149 (MQ=255)                                                                                                                gCTGCAACGCGCGA‑GGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCACGGGTCGACGTCGACGTCGACCTTGGTCTTGCTGTAGCCAAGGGCGGTCGGGCCGTTGACGCTGGCCt                >  2:57320/1‑149 (MQ=255)                                                                                                                 cTGCAACGCGCGA‑GGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCACGGGTCGACGTCGACGTCGACCGTGGTCTTGCTGTAGCCAAGGGCGGTCGGGCCGTTGACGCTGGCCtt               >  1:193313/1‑149 (MQ=255)                                                                                                                  tGCAACGCGCGA‑GGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCACGGGTCGACGTCGACGTCGACCTTGGTCTTGCAGTAGCCAAGGGCGGTCGGGCCGTTGACGCTGGcatag              >  4:82282/1‑145 (MQ=255)                                                                                                                   gCAACGCGCGA‑GGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAg                                                                                                           <  6:28111/55‑1 (MQ=255)                                                                                                                   gCAACGCGCGA‑GGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAg                                                                                                           >  5:28111/1‑55 (MQ=255)                                                                                                                       cgcgcgA‑GGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCACGGGTCGACGTCGACGTCGACCTTGGTCTTGCTGTAGCCAAGGGCGGTCGGGCCGTTGACGCTGGCCTTGGtgtc         <  8:301583/149‑1 (MQ=255)                                                                                                                        gcgcgA‑GGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCACGGGTCGACGTCGACGTCGACCTTGGTCTTGCTGTAGCCAAGGGCGGTCGGGCCGTTGACGCTGGCCTTGGtgtcg        <  4:260970/149‑1 (MQ=255)                                                                                                                         cgcgA‑GGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCACGGGTCGACGTCGACGTCGACCTTGGTCTTGCTGTAGCCAAGGGCGGTCGGGCCGTTGACGCTGGCCTTGGtgtcga       >  5:322309/1‑149 (MQ=255)                                                                                                                          tgca‑GGGCGGCCTCGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCACGGGTCGACGTCGACGTCGACCTTGGTCTTGCTGTAGCCAAGGGCGGTCGGGCCGTTGACGCTGGCCTTGgt            <  3:136655/140‑1 (MQ=255)                                                                                                                          tgca‑GGGCGGCCTCGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCACGGGTCGACGTCGACGTCGACCTTGGTCTTGCTGTAGCCAAGGGCGGTCGGGCCGTTGACGCTGGCCTTGgt            >  4:136655/4‑143 (MQ=255)                                                                                                                             a‑GGGCGGCCTCGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCACGGGTCGACGTCGACGTCGACCTTGGTCTTGCTGTAGCCAAGGGCGGTCGGGCCGTTGACGCTGGc                  >  7:140420/1‑134 (MQ=255)                                                                                                                             a‑GGGCGGCCTCGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCACGGGTCGACGTCGACGTCGACCTTGGTCTTGCTGTAGCCAAGGGCGGTCGGGCCGTTGACGCTGGc                  <  8:140420/134‑1 (MQ=255)                                                                                                                             a‑GGGCGGCCTCGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCACGGGTCGACGTCGACGTCGACCTTGGTCTTGCTGTAGCCAAGGGCGGTCGGGCCGTTGACGCTGGCCTTGGtgtcgatgtc   >  5:339039/1‑149 (MQ=255)                                                                                                                               gggCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCACGGGTCGACGTCGACGTCGACCTTGGTCTTGCTGTAGCCAAGGGCGGTCGGGCCGTTGACGCTGGCCTTGGtgtcgatgtcg  <  1:64832/149‑1 (MQ=255)                                                                                                                               gggCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCACGGGTCGACGTCGACGTCGACCTTGGTCGTGCTGTAGCCAAGGGCGGGCGGGCCGTTGACGCGGGCCTGGGtggcgatggcg  >  8:181133/1‑149 (MQ=255)                                                                                                                                        |                                                                                                                                            CAGCGGTTTGGGGGCGGTGTTGGCGAGCTGGGTGTTTGCACACTGGGGCTGGGTTGCGGTGTGTGGGCTTGGCATGGGCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGA‑GGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCACGGGTCGACGTCGACGTCGACCTTGGTCTTGCTGTAGCCAAGGGCGGTCGGGCCGTTGACGCTGGCCTTGGTGTCGATGTCG  >  NC_002947/593300‑593573

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑