Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 612,541 C→T 9.2% E547E (GAG→GAA)  dxs ← 1‑deoxyxylulose‑5‑phosphate synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947612,5410CT 9.2% 182.6 / 6.8 65E547E (GAG→GAA) dxs1‑deoxyxylulose‑5‑phosphate synthase
Reads supporting (aligned to +/- strand):  ref base C (31/28);  new base T (3/3);  total (34/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.12e-01

CGGCAGCCCCAGGTGCAGAAGCGGCTTGAGCACTGCCTGGCTGGCAAGGAACTCACCCACCGCAGCGCCCGCGCCACCCATGATGGCGTTCTCTTCGATGGTTACCAGCAGCTCATGGCTGCCTGCCAGCTCTAGCACCAGCGCCTCGTCCAGCGGCTTGACGAAGCGCATGTCGACCACCGTGGCGTTGATCTGTTCGGCCACTTGCATG‑GCCTCGGCCAGTTGTACGCCGAACACCAGCAGGGCCACTTTTTCGCCCTGGCGGCGGACCACGCCCTTGCCGATCTCCAGTG  >  NC_002947/612397‑612689
                                                                                                                                                |                                                                                                                                                     
cGGCAGCCCCAGGTGCAGAAGCGGCTTGAGCACTGCCTGGCTGGCAAGGAACTCACCCACCGCAGCGCCCGCGCCACCCATGATGGCGTTCTCTTCGATGGTTACCAGCAGCTCATGGCTGCCTGCCAGCTCTAGCACCAGCGCCTCGt                                                                                                                                                   <  5:8213/149‑1 (MQ=255)
 cgCAGCCCCAGGTGCAGAAGCGGCTTGAGCACTGCCTGTCTGGCAAGGAACTCACCCACCGCAGCGCCCCCGCCACCCATCAGGGCGTTCTCTTCGATGGTTACCAGCAGCTCATGGCTGCCTGCCAGCTCTAGCACCAGCGCCTCGTc                                                                                                                                                  <  4:181148/148‑1 (MQ=255)
     gCCCCAGGTGCAGAAGCGGCTTGAGCACTGCCTGGCTGGCAAGGAACTCACCCACCGCAGCGCCCGCGCCACCCATGATGGCGTTCTCTTCGATGGTTACCAGCAGCTCATGGCTGCCTGCCAGCTCTAGCACCAGCGCCTCGTCCAGc                                                                                                                                              <  6:46998/149‑1 (MQ=255)
       cccAGGTGCAGAAGCGGCTTGAGCACTGCCTGGCTGGCAAGGAACTCACCCACCGCAGCGCCCGCGCCACCCATGATGGCGTTCTCTTCGATGGTTACCAGCAGCTCATGGCTGCCTGCCAGCTCTAGCACCAGCGCCTCGTCCAGCgg                                                                                                                                            >  3:192924/1‑149 (MQ=255)
        ccAGGTGCAGAAGCGGCTTGAGCACTGCCTGGCTGGCAAGGAACTCACCCACCGCAGCGCCCGCGCCACCCATGATGGCGTTCTCTTCGATGGTTACCAGCAGCTCATGGCTGCCTGCCAGCTCTAGCACCAGCGCCTCGTCCAGCGGc                                                                                                                                           >  6:52437/1‑149 (MQ=255)
                aGAAGCGGCTTGAGCACTGCCTGGCTGGCAAGGAACTCACCCACCGCAGCGCCCGCGCCACCCATGATGGCGTTCTCTTCGATGGTTACCAGCAGCTCATGGCTGCCTGCCAGCTCTAGCACCAGCGCCTCGTCCAGCGGCTTGACGaa                                                                                                                                   <  7:67404/149‑1 (MQ=255)
                 gAAGCGGCTTGAGCACTGCCTGGCTGGCAAGGAACTCACCCACCGCAGCGCCCGCGCCACCCATGATGGCGTTCTCTTCGATGGTTACCAGCAGCTCATGGCTGCCTGCCAGCTCTAGCGCCAGCGCCTCGTCCa                                                                                                                                                <  4:10937/135‑1 (MQ=255)
                 gAAGCGGCTTGAGCACTGCCTGGCTGGCAAGGAACTCACCCACCGCAGCGCCCGCGCCACCCATGATGGCGTTCTCTTCGATGGTTACCAGCAGCTCATGGCTGCCTGCCAGCTCTAGCGCCAGCGCCTCGTCCa                                                                                                                                                >  3:10937/1‑135 (MQ=255)
                 gAAGCGGCTTGAGCACTGCCTGGCTGGCAAGGAACTCACCCACCGCAGCGCCCGCGCCACCCATGATGGCGTTCTCTTCGATGGTTACCAGCAGCTCATGGCTGCCTGCCAGCTCTAGCACCAGCGCCTCGTCCAGCGGCTTGACGAAg                                                                                                                                  <  3:353584/149‑1 (MQ=255)
                        cTTGAGCACTGCCTGGCTGGCAAGGAACTCACCCACCGCAGCGCCCGCGCCACCCATGATGGCGTTCTCTTCGATGGTTACCAGCAGCTCATGGCTGCCTGCCAGCTCTAGCACCAGCGCTTCGTCAAGCCGCTGGACGAGgcgctggt                                                                                                                           <  5:146150/149‑5 (MQ=255)
                         ttGAGCACTGCCTGGCTGGCAAGGAACTCACCCACCGCAGCGCCCGCGCCACCCATGATGGCGTTCTCTTCGATGGTTACCAGCAGCTCATGGCTGCCTGCCAGCTCTAGCACCAGCGCCTCGTCCAGCGGCTTGACGAAGCGCATGTc                                                                                                                          >  4:338229/1‑149 (MQ=255)
                           gAGCACTGCCTGGCTGGCAAGGAACTCACCCACCGCAGCGCCCGCGCCACCCATGATGGCGTTCTCTTCGATGGTTACCAGCAGCTCATGGCTGCCTGCCAGCTCTAGCACCAGCGCTTCGTCAAGCCGCTGGACGAGgcgctggtg                                                                                                                          <  7:166008/147‑6 (MQ=255)
                                             aaGGAACTCACCCACCGCAGCGCCCGCGCCACCCATGATGGCGTTCTCTTCGATGGTTACCAGCAGCTCATGGCTGCCTTCCAGCTCTAGCACCAGCGCCTCGTCCAGCGGCTTGACGAAGCGCATGTCGACCACCGTGGCGTTGATCt                                                                                                      <  4:180789/149‑1 (MQ=255)
                                                    tCACCCACCGCAGCGCCCGCGCCACCCATGATGGCGTTCTCTTCGATGGTTACCAGCAGCTCATGGCTGCCTGCCAGCTCTAGCACCAGCGCCTCGTCCAGCGGCTTGACGAAGCGCATGTCGACCACCGTGGCGTTGATCTGTTCGGc                                                                                               >  2:257625/1‑149 (MQ=255)
                                                     cacccaccGCAGCGCCCGCGCCACCCATGATGGCGTTCTCTTCGATGGTTACCAGCAGCTCATGGCTGCCTGCCAGCTCTAGCACCAGCGCCTCGTCCAGCGGCTTGACGAAGCGCATGTCGACCACCGTGGCGTTGATCTGTTCGGc                                                                                               >  1:163142/1‑148 (MQ=255)
                                                      acccaccGCAGCGCCCGCGCCACCCATGATGGCGTTCTCTTCGATGGTTACCAGCAGCTCATGGCTGCCTGCCAGCTCTAGCACCAGCGCCTCGTCCAGCGGCTTGACGAAGCGCATGTCGACCACCGTGGCGTTGATCTGTTCGGCCa                                                                                             >  3:321211/1‑149 (MQ=255)
                                                       cccaccGCAGCGCCCGCGCCACCCATGATGGCGTTCTCTTCGATGGTTACCAGCAGCTCATGGCTGCCTGCCAGCTCTAGCACCAGCGCCTCGTCCAGCGGCTTGACGAAGCGCATGTCGACCACCGTGGCGTTGATCTGTTCGGCCAc                                                                                            >  3:152354/1‑149 (MQ=255)
                                                             gCAGCGCCCGCGCCACCCATGATGGCGTTCTCTTCGATGGTTACCAGCAGCTCATGGCTGCCTGCCAGCTCTAGCACCAGCGCCTCGTCCAGCGGCTTGACGAAGCGCATGTCGACCACCGTGGCGTTGATCTGTTCGGCCACTTGCAt                                                                                      <  4:152354/149‑1 (MQ=255)
                                                              cAGCGCCCGCGCCACCCATGATGGCGTTCTCTTCGATGGTTACCAGCAGCTCATGGCTGCCTGCCAGCTCTAGCACCAGCGCCTCGTCCAGCGGCTTGACGAAGCGCATGTCGacca                                                                                                                     <  7:123989/117‑1 (MQ=255)
                                                              cAGCGCCCGCGCCACCCATGATGGCGTTCTCTTCGATGGTTACCAGCAGCTCATGGCTGCCTGCCAGCTCTAGCACCAGCGCCTCGTCCAGCGGCTTGACGAAGCGCATGTCGacca                                                                                                                     >  8:123989/1‑117 (MQ=255)
                                                              cAGCGCCCGCGCCACCCATGATGGCGTTCTCTTCGATGGTTACCAGCAGCTCATGGCTGCCTGCCAGCTCTAGCACCAGCGCCTCGTCCAGCGGCTTGACGAAGCGCATGTCGACCACCGTGGCGTTGATCTGTTCGGCCACTTGCATg                                                                                     <  4:321211/149‑1 (MQ=255)
                                                                          cACCCATGATGGCGTTCTCTTCGATGGTTACCAGCAGCTCATGGCTGCCTGCCAGCTCTAGCACCACCGCCTCGTCCAGCGGCTTGACGAAGCGCATGTCGACCACCGTGGCGTTGATCTGTTCGGCCACTTGCATG‑GCCTCGGCCAGt                                                                        >  8:124069/1‑149 (MQ=255)
                                                                           aCCCATGATGGCGTTCTCTTCGATGGTTACCAGCAGCTCATGGCTGCCTGCCAGCTCTAGCACCAGCGCCTCGTCCAGCGGCTTGACGAAGCGCATGTCGACCACCGTGGCGTTGATCTGTTCGGCCACTTGCATG‑GCCTCGGCCAGtt                                                                       <  5:52437/149‑1 (MQ=255)
                                                                           aCCCATGATGGCGTTCTCTTCGATGGTTACCAGCAGCTCATGGCTGCCTGCCAGCTCTAGCACCAGCGCCTCGTCCAGCGGCTTGACGAAGCGCATGTCGACCACCGTGGCGTTGATCTGTTCGGCCACTTGCATG‑GCCTCGGCCAGtt                                                                       >  5:291361/1‑149 (MQ=255)
                                                                               atgatgGCGTTCTCTTCGATGGTTACCAGCAGCTCATGGCTGCCTGCGAGCTCTAGCTCCAGCGCCTCGTCCAGCGGCTTGACGAAGCGCATGTCGTCCACGGTGGCGTTGATCTGTTCGGCCACTTGCATG‑GCCTCGGCCAGTTGTAc                                                                   <  5:112177/149‑1 (MQ=255)
                                                                               atgatgGCGTTCTCTTCGATGGTTACCAGCAGCTCATGGCTGCCTGCCAGCTCTAGCACCAGCGCCTCGTCCAGCGGCTTGACGAAGCGCATGTCGACCACCGTGGCGTTGATCTGTTCGGCCACTTGCATG‑GCCTCGGCCAGTTGTAc                                                                   <  3:338229/149‑1 (MQ=255)
                                                                               atgatgGCGTTCTCTTCGATGGTTACCAGCAGCTCATGGCTGCCTGCCAGCTCTAGCACCAGCGCCTCGTCCAGCGGCTTGACGAAGCGCATGTCGACCACCGTGGCGTTGATCTGTTCGGCCACTTGCATG‑GCCTCGGCCAGTTGTAc                                                                   >  6:182738/1‑149 (MQ=255)
                                                                                 gatgGCGTTCTCTTCGATGGTTACCAGCAGCTCATGGCTGCCTGCCAGCTCTAGCACCAGCGCCTCGTCCAGCGGCTTGACGAAGCGCATGTCGACCACCGTGGCGTTGATCTGTTCGGCCACTTGCATG‑GCCTCGGc                                                                            <  6:264670/138‑1 (MQ=255)
                                                                                 gatgGCGTTCTCTTCGATGGTTACCAGCAGCTCATGGCTGCCTGCCAGCTCTAGCACCAGCGCCTCGTCCAGCGGCTTGACGAAGCGCATGTCGACCACCGTGGCGTTGATCTGTTCGGCCACTTGCATG‑GCCTCGGc                                                                            >  5:264670/1‑138 (MQ=255)
                                                                                        ttCTCTTCGATGGTTACCAGCAGCTCATGGCTGCCTGCCAGCTCTAGCACCAGCGCCTCGTCCAGCGGCTTGACGAAGCGCATGTCGACCACCGTGGCGTTGATCTGTTCGGCCACTTGCATG‑GCCTCGGCCAGTTGTACGCCGAacac                                                          <  4:154820/149‑1 (MQ=255)
                                                                                          ctctTCGATGGTTACCAGCAGCTCATGGCTGCCTGCCAGCTCTAGCACCAGCGCCTCGTCCAGCGGCTTGACGAAGCGCATGTCGACCACCGTGGCGTTGATCTGTTCGGCCACTTGCATG‑GCCTCGGCCAGTTGTACGCCGAacac                                                          >  4:250416/1‑147 (MQ=255)
                                                                                          ctctTCGATGGTTACCAGCAGCTCATGGCTGCCTGCCAGCTCTAGCACCAGCGCCTCGTCCAGCGGCTTGACGAAGCGCATGTCGACCACCGTGGCGTTGATCTGTTCGGCCACTTGCATG‑GCCTCGGCCAGTTGTACGCCGAACAcc                                                         <  2:163142/148‑1 (MQ=255)
                                                                                           tctTCGATGGTTACCAGCAGCTCATGGCTGCCTGCCAGCTCTAGCACCAGCGCCTCGTCCAGCGGCTTGACGAAGCGCATGTCGACCACCGTGGCGTTGATCTGTTCGGCCACTTGCATG‑GCCTCGGCCAGTTGTACGCCGAACACcag                                                       >  5:210539/1‑149 (MQ=255)
                                                                                             ttCGATGGTTACCAGCAGCTCATGGCTGCCTGCCAGCTCTAGCACCAGCGCCTCGTCCAGCGGCTTGACGAAGCGCATGTCGACCACCGTGGCGTTGATCTGTTCGGCCACTTGCATG‑GCCTCGGCCAGTTGTACGCCGAACACcag                                                       >  7:290568/1‑147 (MQ=255)
                                                                                              tCGATGGTTACCAGCAGCTCATGGCTGCCTGCCAGCTCTAGCACCAGCGCCTCGTCCAGCGGCTTGACGAAGCGCATGTCGACCACCGTGGCGTTGATCTGTTCGGCCACtt                                                                                          <  5:23282/112‑1 (MQ=255)
                                                                                              tCGATGGTTACCAGCAGCTCATGGCTGCCTGCCAGCTCTAGCACCAGCGCCTCGTCCAGCGGCTTGACGAAGCGCATGTCGACCACCGTGGCGTTGATCTGTTCGGCCACtt                                                                                          >  6:23282/1‑112 (MQ=255)
                                                                                                gATGGTTACCAGCAGCTCATGGCTGCCTGCCAGCTCTAGCACCAGCGCCTCGTCCAGCGGCTTGACGAAGCGCATGTCGACCACCGTGGCGTTGATCTGTTCGGCCACTTGCATG‑GCCTCGGCCAGTTGTACGCCGAACACCAGCAggg                                                  <  8:290568/149‑1 (MQ=255)
                                                                                                gATGGTTACCAGCAGCTCATGGCTGCCTGCCAGCTCTAGCACCAGCGCCTCGTCCAGCGGCTTGACGAAGCGCATGTCGACCACCGTGGCGTTGATCTGTTCGGCCACTTGCATG‑GCCTCGGCCAGTTGTACGCCGAACACCAGCAgg                                                   <  1:46674/148‑1 (MQ=255)
                                                                                                       aCCAGCAGCTCATGGCTGCCTGCCAGCTCTAGCACCAGCGCCTCGTCCAGCGGCTTGACGAAGCGCATGTCGACCACCGTGGCGTTGATCTGTTCGGCCACTTGCATG‑GCCTCGGCCAGTTg                                                                      >  4:298806/1‑122 (MQ=255)
                                                                                                        ccAGCAGCTCATGGCTGCCTGCCAGCTCTAGCACCAGCGCCTCGTCCAGCGGCTTGACGAAGCGCATGTCGACCACCGTGGCGTTGATCTGTTCGGCCACTTGCATG‑GCCTCGGCCAGTTGTACGCCGAACACCAGCAGGGCCACttt                                           <  4:7552/148‑1 (MQ=255)
                                                                                                            cagcTCATGGCTGCCTGCCAGCTCTAGCACCAGCGCCTCGTCCAGCGGCTTGACGAAGCGCATGTCGACCACCGTGGCGTTGATCTGTTCGGCCACTTGCATGCGCCTCGGCCAGTTGTACGCCGAACACCAGCAGGGCCACTTTTTCg                                       <  1:257625/149‑1 (MQ=255)
                                                                                                                  aTGGCTGCCTGCCAGCTCTAGCACCAGCGCCTCGTCCAGCGGCTTGACGAAGCGCATGTCGACCACCGTGGCGTTGATCTGTTCTGCCAGTTGCATG‑GCCTCGGCCAGTTGTACGCCGAACACCAGCAGGGCCACTTTTTCGCCCTg                                  <  6:210539/147‑1 (MQ=255)
                                                                                                                  aTGGCTGCCTGCCAGCTCTAGCACCAGCGCCTCGTCCAGCGGCTTGACGAAGCGCATGTCGACCACCGTGGCGTTGATCTGTTCGGCCACTTGCATG‑GCCTCGGCCAGTTGTACGCCGAACACCAGCAGGGc                                                 <  1:171590/132‑1 (MQ=255)
                                                                                                                  aTGGCTGCCTGCCAGCTCTAGCACCAGCGCCTCGTCCAGCGGCTTGACGAAGCGCATGTCGACCACCGTGGCGTTGATCTGTTCGGCCACTTGCATG‑GCCTCGGCCAGTTGTACGCCGAACACCAGCAGGGc                                                 >  2:171590/1‑132 (MQ=255)
                                                                                                                  aTGGCTGCCTGCCAGCTCTAGCACCAGCGCCTCGTCCAGCGGCTTGACGAAGCGCATGTCGACCACCGTGGCGTTGATCTGTTCGGCCACTTGCATG‑GCCTCGGCCAGTTGTACGCCGAACACCAGCAGGGCCAc                                              >  4:253566/1‑135 (MQ=255)
                                                                                                                  aTGGCTGCCTGCCAGCTCTAGCACCAGCGCCTCGTCCAGCGGCTTGACGAAGCGCATGTCGACCACCGTGGCGTTGATCTGTTCGGCCACTTGCATG‑GCCTCGGCCAGTTGTACGCCGAACACCAGCAGGGCCAc                                              <  3:253566/135‑1 (MQ=255)
                                                                                                                  aTGGCTGCCTGCCAGCTCTAGCACCAGCGCCTCGTCCAGCGGCTTGACGAAGCGCATGTCGACCACCGTGGCGTTGATCTGTTCGGCCACTTGCATG‑GCCTCGGCCAGTTGTACGCCGAACACCAGCAGGGCCACTTTTTCGCCCTgg                                 <  5:182738/148‑1 (MQ=255)
                                                                                                                      ctgcctgcCAGCTCTAGCACCAGCGCCTCGTCCAGCGGCTTGACGAAGCGCATGTCGACCACCGTGGCGTTGATCTGTACGGCCACTTGCATG‑GCCTCGGCCAGTTGTACGCCGAACACCAGCAGGGCCACTTTTTCGCCCTGgcggcg                            <  4:192924/149‑1 (MQ=255)
                                                                                                                       tgcctgccAGCTCTAGCACCAGCGCCTCGTCCAGCGGCTTGACGAAGCGCATGTCGACCACCGTGGCGTTGATCTGTTCGGCCACTTGCATG‑GCCTCGGCCAGTTGTACGCCGAACACCAGCAGGGCCACTTTTTCGCCCTGGcggcgg                           >  3:264161/1‑149 (MQ=255)
                                                                                                                        gcctgccAGCTCTAGCACCAGCGCCTCGTCCAGCGGCTTGACGAAGCGCATGTCGACCACCGTGGCGTTGATCTGTTCGGCCACTTGCATG‑GCCTCGGCCAGTTGTACGCCGAACACCAGCAGGGCCACTTTTTCGCCCTGGCGGCGGa                          >  7:75638/1‑149 (MQ=255)
                                                                                                                             ccAGCTCTAGCACCAGCGCCTCGTCCAGCGGCTTGACGAAGCGCATGTCGACCACCGTGGCGTTGATCTGTTCGGCCACTTGCATG‑GCCTCGGCCAGTTGTACGCCGAACACCAGCAGGGCCACTTTTTCGCCCTGGCGGCGGACCACg                     >  2:114395/1‑149 (MQ=255)
                                                                                                                             ccAGCTCTAGCACCAGCGCCTCGTCCAGCGGCTTGACGAAGCGCATGTCGACCACCGTGGCGTTGATCTGTTCGGCCACTTGCATG‑GCCTCGGCCAGTTGTACGCCGAACACCAGCAGGGCCACTTTTTCGCCCTGGCGGCGGACCACg                     >  1:208237/1‑149 (MQ=255)
                                                                                                                                 ctctAGCACCAGCGCCTCGTCCAGCGGCTTGACGAAGCGCATGTCGACCACCGTGGCGTTGATCTGTTCGGCCACTTGCATG‑GCCTCGGCCAGTTGTACGCCGAACACCAGCAGGGCCACTTTTTCGCCCTGGCGGCGGACCACGCCCt                 >  8:279241/1‑149 (MQ=255)
                                                                                                                                    tAGCACCAGCGCCTCGTCCAGCGGCTTGACGAAGCGCATGTCGACCACCGTGGCGTTGATCTGTTCGGCCACTTGCATG‑GCCTCGGCCAGTTGTACGCCGAACACCAGCAGGGCCACTTTTTCGCCCTGGCGGCGGACCACGCCCTTGc              >  3:114302/1‑149 (MQ=255)
                                                                                                                                        aCCAGCGCCTCGTCCAGCGGCTTGACGAAGCGCATGTCGACCACCGTGGCGTTGATCTGTTCGGCCACTTGCATG‑GCCTCGGCCAGTTGTACGCCGAACACCAGCAGGGCCACTTTTTCGCCCTGGCGGCGGACCACGCCCTTGCCGAt          >  6:353714/1‑149 (MQ=255)
                                                                                                                                         ccAGCGCCTCGTCCAGCGGCTTGACGAAGCGCATGTCGACCACCGTGGCGTTGATCTGTTCGGCCACTTGCATG‑GCCTCGGCCAGTTGTACGCCGAACACCAGCAGGGCCACTTTTTCGCCCTGGCGGCGGACCACGCCCTTGCCGAtc         >  3:146058/1‑149 (MQ=255)
                                                                                                                                         catgcgcTTCGTCAAGCCGCTGGACGAGGCGCATGTCGACCACCGTGGCGTTGATCTGTTCGGCCACTTGCATG‑GCCTCGGCCAGTTGTACGCCGAACACCAGCAGGGCCACTTTTTCGCCCTGGCGGCGGACCACGcc                   <  8:98574/136‑1 (MQ=255)
                                                                                                                                         catgcgcTTCGTCAAGCCGCTGGACGAGGCGCATGTCGACCACCGTGGCGTTGATCTGTTCGGCCACTTGCATG‑GCCTCGGCCAGTTGTACGCCGAACACCAGCAGGGCCACTTTTTCGCCCTGGCGGCGGACCACGcc                   >  7:98574/4‑139 (MQ=255)
                                                                                                                                         catgcgcTTCGTCAAGCCGCTGGACGAGGCGCATGTCGACCACCGTGGCGTTGATCTGTTCGGCCACTTGCATG‑GCCTCGGCCAGTTGTACGCCGAACACCAGCAGGGCCACTTTTTCGCCCTGGCGGCGGACCACGCCCTTGCCGAtc         >  7:333188/4‑149 (MQ=255)
                                                                                                                                         catgcgcTTCGTCAAGCCGCTGGACGAGGCGCATGTCGACCACCGTGGCGTTGATCTGTTAGGCCACTTGCATG‑GCCTCGGCCAGTTGTACGCCGAACACCAGCAGGGCCACTTTTTCGCCCTGGCGGCGGACCACGCCCTTGCCGAtc         >  5:270907/4‑149 (MQ=255)
                                                                                                                                          cAGCGCCTCGTCCAGCGGCTTGACGAAGCGCATGTCGACCACCGTGGCGTTGATCTGTTCGGc                                                                                               <  1:34387/63‑1 (MQ=255)
                                                                                                                                          cAGCGCCTCGTCCAGCGGCTTGACGAAGCGCATGTCGACCACCGTGGCGTTGATCTGTTCGGc                                                                                               >  2:34387/1‑63 (MQ=255)
                                                                                                                                          cAGCGCCTCGTCCAGCGGCTTGACGAAGCGCATGTCGACCACCGTGGCGTTGATCTGTTCGGCCACTTGCATG‑GCCTCGGCCAGTTGTACGCCGAACACCAGCAGGGc                                                 >  6:2748/1‑108 (MQ=255)
                                                                                                                                          cAGCGCCTCGTCCAGCGGCTTGACGAAGCGCATGTCGACCACCGTGGCGTTGATCTGTTCGGCCACTTGCATG‑GCCTCGGCCAGTTGTACGCCGAACACCAGCAGGGc                                                 <  5:2748/108‑1 (MQ=255)
                                                                                                                                            gcgcCTCGTCCAGCGGCTTGACGAAGCGCATGTCGACCACCGTGGCGTTGATCTGTTCGGCCACTTGCATG‑GCCTCGGCCAGTTGTACGCCGAACACCAGCAGGGCCACTTTTTCGCCCTGGCGGCGGACCACGCCCTTGCCGAtctc       >  8:281000/1‑148 (MQ=255)
                                                                                                                                                cTCGTCCAGCGGCTTGACGAAGCGCATGTCGACCACCGTGGCGTTGATCTGTTCGGCCACTTGCATG‑GCCTCGGCCAGTTGTACGCCGAACACCAGCAGGGCCACTTTTTCGCCCTGGCGGCGGACCACGCCCTTGCCGATCTCCAGTg  <  8:75638/149‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                     
CGGCAGCCCCAGGTGCAGAAGCGGCTTGAGCACTGCCTGGCTGGCAAGGAACTCACCCACCGCAGCGCCCGCGCCACCCATGATGGCGTTCTCTTCGATGGTTACCAGCAGCTCATGGCTGCCTGCCAGCTCTAGCACCAGCGCCTCGTCCAGCGGCTTGACGAAGCGCATGTCGACCACCGTGGCGTTGATCTGTTCGGCCACTTGCATG‑GCCTCGGCCAGTTGTACGCCGAACACCAGCAGGGCCACTTTTTCGCCCTGGCGGCGGACCACGCCCTTGCCGATCTCCAGTG  >  NC_002947/612397‑612689

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑