Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 612,547 C→A 9.4% L545L (CTG→CTT)  dxs ← 1‑deoxyxylulose‑5‑phosphate synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947612,5470CA 9.4% 189.5 / 9.4 64L545L (CTG→CTT) dxs1‑deoxyxylulose‑5‑phosphate synthase
Reads supporting (aligned to +/- strand):  ref base C (31/27);  new base A (3/3);  total (34/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.40e-01

GCCCCAGGTGCAGAAGCGGCTTGAGCACTGCCTGGCTGGCAAGGAACTCACCCACCGCAGCGCCCGCGCCACCCATGATGGCGTTCTCTTCGATGGTTACCAGCAGCTCATGGCTGCCTGCCAGCTCTAGCACCAGCGCCTCGTCCAGCGGCTTGACGAAGCGCATGTCGACCACCGTGGCGTTGATCTGTTCGGCCACTTGCATG‑GCCTCGGCCAGTTGTACGCCGAACACCAGCAGGGCCACTTTTTCGCCCTGGCGGCGGACCACGCCCTTGCCGATCTCCAGTGGTTGC  >  NC_002947/612402‑612694
                                                                                                                                                 |                                                                                                                                                    
gCCCCAGGTGCAGAAGCGGCTTGAGCACTGCCTGGCTGGCAAGGAACTCACCCACCGCAGCGCCCGCGCCACCCATGATGGCGTTCTCTTCGATGGTTACCAGCAGCTCATGGCTGCCTGCCAGCTCTAGCACCAGCGCCTCGTCCAGc                                                                                                                                                   <  6:46998/149‑1 (MQ=255)
  cccAGGTGCAGAAGCGGCTTGAGCACTGCCTGGCTGGCAAGGAACTCACCCACCGCAGCGCCCGCGCCACCCATGATGGCGTTCTCTTCGATGGTTACCAGCAGCTCATGGCTGCCTGCCAGCTCTAGCACCAGCGCCTCGTCCAGCgg                                                                                                                                                 >  3:192924/1‑149 (MQ=255)
   ccAGGTGCAGAAGCGGCTTGAGCACTGCCTGGCTGGCAAGGAACTCACCCACCGCAGCGCCCGCGCCACCCATGATGGCGTTCTCTTCGATGGTTACCAGCAGCTCATGGCTGCCTGCCAGCTCTAGCACCAGCGCCTCGTCCAGCGGc                                                                                                                                                >  6:52437/1‑149 (MQ=255)
           aGAAGCGGCTTGAGCACTGCCTGGCTGGCAAGGAACTCACCCACCGCAGCGCCCGCGCCACCCATGATGGCGTTCTCTTCGATGGTTACCAGCAGCTCATGGCTGCCTGCCAGCTCTAGCACCAGCGCCTCGTCCAGCGGCTTGACGaa                                                                                                                                        <  7:67404/149‑1 (MQ=255)
            gAAGCGGCTTGAGCACTGCCTGGCTGGCAAGGAACTCACCCACCGCAGCGCCCGCGCCACCCATGATGGCGTTCTCTTCGATGGTTACCAGCAGCTCATGGCTGCCTGCCAGCTCTAGCGCCAGCGCCTCGTCCa                                                                                                                                                     <  4:10937/135‑1 (MQ=255)
            gAAGCGGCTTGAGCACTGCCTGGCTGGCAAGGAACTCACCCACCGCAGCGCCCGCGCCACCCATGATGGCGTTCTCTTCGATGGTTACCAGCAGCTCATGGCTGCCTGCCAGCTCTAGCGCCAGCGCCTCGTCCa                                                                                                                                                     >  3:10937/1‑135 (MQ=255)
            gAAGCGGCTTGAGCACTGCCTGGCTGGCAAGGAACTCACCCACCGCAGCGCCCGCGCCACCCATGATGGCGTTCTCTTCGATGGTTACCAGCAGCTCATGGCTGCCTGCCAGCTCTAGCACCAGCGCCTCGTCCAGCGGCTTGACGAAg                                                                                                                                       <  3:353584/149‑1 (MQ=255)
                   cTTGAGCACTGCCTGGCTGGCAAGGAACTCACCCACCGCAGCGCCCGCGCCACCCATGATGGCGTTCTCTTCGATGGTTACCAGCAGCTCATGGCTGCCTGCCAGCTCTAGCACCAGCGCTTCGTCAAGCCGCTGGACGAGgcgctggt                                                                                                                                <  5:146150/149‑5 (MQ=255)
                    ttGAGCACTGCCTGGCTGGCAAGGAACTCACCCACCGCAGCGCCCGCGCCACCCATGATGGCGTTCTCTTCGATGGTTACCAGCAGCTCATGGCTGCCTGCCAGCTCTAGCACCAGCGCCTCGTCCAGCGGCTTGACGAAGCGCATGTc                                                                                                                               >  4:338229/1‑149 (MQ=255)
                      gAGCACTGCCTGGCTGGCAAGGAACTCACCCACCGCAGCGCCCGCGCCACCCATGATGGCGTTCTCTTCGATGGTTACCAGCAGCTCATGGCTGCCTGCCAGCTCTAGCACCAGCGCTTCGTCAAGCCGCTGGACGAGgcgctggtg                                                                                                                               <  7:166008/147‑6 (MQ=255)
                                        aaGGAACTCACCCACCGCAGCGCCCGCGCCACCCATGATGGCGTTCTCTTCGATGGTTACCAGCAGCTCATGGCTGCCTTCCAGCTCTAGCACCAGCGCCTCGTCCAGCGGCTTGACGAAGCGCATGTCGACCACCGTGGCGTTGATCt                                                                                                           <  4:180789/149‑1 (MQ=255)
                                               tCACCCACCGCAGCGCCCGCGCCACCCATGATGGCGTTCTCTTCGATGGTTACCAGCAGCTCATGGCTGCCTGCCAGCTCTAGCACCAGCGCCTCGTCCAGCGGCTTGACGAAGCGCATGTCGACCACCGTGGCGTTGATCTGTTCGGc                                                                                                    >  2:257625/1‑149 (MQ=255)
                                                cacccaccGCAGCGCCCGCGCCACCCATGATGGCGTTCTCTTCGATGGTTACCAGCAGCTCATGGCTGCCTGCCAGCTCTAGCACCAGCGCCTCGTCCAGCGGCTTGACGAAGCGCATGTCGACCACCGTGGCGTTGATCTGTTCGGc                                                                                                    >  1:163142/1‑148 (MQ=255)
                                                 acccaccGCAGCGCCCGCGCCACCCATGATGGCGTTCTCTTCGATGGTTACCAGCAGCTCATGGCTGCCTGCCAGCTCTAGCACCAGCGCCTCGTCCAGCGGCTTGACGAAGCGCATGTCGACCACCGTGGCGTTGATCTGTTCGGCCa                                                                                                  >  3:321211/1‑149 (MQ=255)
                                                  cccaccGCAGCGCCCGCGCCACCCATGATGGCGTTCTCTTCGATGGTTACCAGCAGCTCATGGCTGCCTGCCAGCTCTAGCACCAGCGCCTCGTCCAGCGGCTTGACGAAGCGCATGTCGACCACCGTGGCGTTGATCTGTTCGGCCAc                                                                                                 >  3:152354/1‑149 (MQ=255)
                                                        gCAGCGCCCGCGCCACCCATGATGGCGTTCTCTTCGATGGTTACCAGCAGCTCATGGCTGCCTGCCAGCTCTAGCACCAGCGCCTCGTCCAGCGGCTTGACGAAGCGCATGTCGACCACCGTGGCGTTGATCTGTTCGGCCACTTGCAt                                                                                           <  4:152354/149‑1 (MQ=255)
                                                         cAGCGCCCGCGCCACCCATGATGGCGTTCTCTTCGATGGTTACCAGCAGCTCATGGCTGCCTGCCAGCTCTAGCACCAGCGCCTCGTCCAGCGGCTTGACGAAGCGCATGTCGacca                                                                                                                          <  7:123989/117‑1 (MQ=255)
                                                         cAGCGCCCGCGCCACCCATGATGGCGTTCTCTTCGATGGTTACCAGCAGCTCATGGCTGCCTGCCAGCTCTAGCACCAGCGCCTCGTCCAGCGGCTTGACGAAGCGCATGTCGacca                                                                                                                          >  8:123989/1‑117 (MQ=255)
                                                         cAGCGCCCGCGCCACCCATGATGGCGTTCTCTTCGATGGTTACCAGCAGCTCATGGCTGCCTGCCAGCTCTAGCACCAGCGCCTCGTCCAGCGGCTTGACGAAGCGCATGTCGACCACCGTGGCGTTGATCTGTTCGGCCACTTGCATg                                                                                          <  4:321211/149‑1 (MQ=255)
                                                                     cACCCATGATGGCGTTCTCTTCGATGGTTACCAGCAGCTCATGGCTGCCTGCCAGCTCTAGCACCACCGCCTCGTCCAGCGGCTTGACGAAGCGCATGTCGACCACCGTGGCGTTGATCTGTTCGGCCACTTGCATG‑GCCTCGGCCAGt                                                                             >  8:124069/1‑149 (MQ=255)
                                                                      aCCCATGATGGCGTTCTCTTCGATGGTTACCAGCAGCTCATGGCTGCCTGCCAGCTCTAGCACCAGCGCCTCGTCCAGCGGCTTGACGAAGCGCATGTCGACCACCGTGGCGTTGATCTGTTCGGCCACTTGCATG‑GCCTCGGCCAGtt                                                                            >  5:291361/1‑149 (MQ=255)
                                                                      aCCCATGATGGCGTTCTCTTCGATGGTTACCAGCAGCTCATGGCTGCCTGCCAGCTCTAGCACCAGCGCCTCGTCCAGCGGCTTGACGAAGCGCATGTCGACCACCGTGGCGTTGATCTGTTCGGCCACTTGCATG‑GCCTCGGCCAGtt                                                                            <  5:52437/149‑1 (MQ=255)
                                                                          atgatgGCGTTCTCTTCGATGGTTACCAGCAGCTCATGGCTGCCTGCGAGCTCTAGCTCCAGCGCCTCGTCCAGCGGCTTGACGAAGCGCATGTCGTCCACGGTGGCGTTGATCTGTTCGGCCACTTGCATG‑GCCTCGGCCAGTTGTAc                                                                        <  5:112177/149‑1 (MQ=255)
                                                                          atgatgGCGTTCTCTTCGATGGTTACCAGCAGCTCATGGCTGCCTGCCAGCTCTAGCACCAGCGCCTCGTCCAGCGGCTTGACGAAGCGCATGTCGACCACCGTGGCGTTGATCTGTTCGGCCACTTGCATG‑GCCTCGGCCAGTTGTAc                                                                        >  6:182738/1‑149 (MQ=255)
                                                                          atgatgGCGTTCTCTTCGATGGTTACCAGCAGCTCATGGCTGCCTGCCAGCTCTAGCACCAGCGCCTCGTCCAGCGGCTTGACGAAGCGCATGTCGACCACCGTGGCGTTGATCTGTTCGGCCACTTGCATG‑GCCTCGGCCAGTTGTAc                                                                        <  3:338229/149‑1 (MQ=255)
                                                                            gatgGCGTTCTCTTCGATGGTTACCAGCAGCTCATGGCTGCCTGCCAGCTCTAGCACCAGCGCCTCGTCCAGCGGCTTGACGAAGCGCATGTCGACCACCGTGGCGTTGATCTGTTCGGCCACTTGCATG‑GCCTCGGc                                                                                 <  6:264670/138‑1 (MQ=255)
                                                                            gatgGCGTTCTCTTCGATGGTTACCAGCAGCTCATGGCTGCCTGCCAGCTCTAGCACCAGCGCCTCGTCCAGCGGCTTGACGAAGCGCATGTCGACCACCGTGGCGTTGATCTGTTCGGCCACTTGCATG‑GCCTCGGc                                                                                 >  5:264670/1‑138 (MQ=255)
                                                                                   ttCTCTTCGATGGTTACCAGCAGCTCATGGCTGCCTGCCAGCTCTAGCACCAGCGCCTCGTCCAGCGGCTTGACGAAGCGCATGTCGACCACCGTGGCGTTGATCTGTTCGGCCACTTGCATG‑GCCTCGGCCAGTTGTACGCCGAacac                                                               <  4:154820/149‑1 (MQ=255)
                                                                                     ctctTCGATGGTTACCAGCAGCTCATGGCTGCCTGCCAGCTCTAGCACCAGCGCCTCGTCCAGCGGCTTGACGAAGCGCATGTCGACCACCGTGGCGTTGATCTGTTCGGCCACTTGCATG‑GCCTCGGCCAGTTGTACGCCGAacac                                                               >  4:250416/1‑147 (MQ=255)
                                                                                     ctctTCGATGGTTACCAGCAGCTCATGGCTGCCTGCCAGCTCTAGCACCAGCGCCTCGTCCAGCGGCTTGACGAAGCGCATGTCGACCACCGTGGCGTTGATCTGTTCGGCCACTTGCATG‑GCCTCGGCCAGTTGTACGCCGAACAcc                                                              <  2:163142/148‑1 (MQ=255)
                                                                                      tctTCGATGGTTACCAGCAGCTCATGGCTGCCTGCCAGCTCTAGCACCAGCGCCTCGTCCAGCGGCTTGACGAAGCGCATGTCGACCACCGTGGCGTTGATCTGTTCGGCCACTTGCATG‑GCCTCGGCCAGTTGTACGCCGAACACcag                                                            >  5:210539/1‑149 (MQ=255)
                                                                                        ttCGATGGTTACCAGCAGCTCATGGCTGCCTGCCAGCTCTAGCACCAGCGCCTCGTCCAGCGGCTTGACGAAGCGCATGTCGACCACCGTGGCGTTGATCTGTTCGGCCACTTGCATG‑GCCTCGGCCAGTTGTACGCCGAACACcag                                                            >  7:290568/1‑147 (MQ=255)
                                                                                         tCGATGGTTACCAGCAGCTCATGGCTGCCTGCCAGCTCTAGCACCAGCGCCTCGTCCAGCGGCTTGACGAAGCGCATGTCGACCACCGTGGCGTTGATCTGTTCGGCCACtt                                                                                               <  5:23282/112‑1 (MQ=255)
                                                                                         tCGATGGTTACCAGCAGCTCATGGCTGCCTGCCAGCTCTAGCACCAGCGCCTCGTCCAGCGGCTTGACGAAGCGCATGTCGACCACCGTGGCGTTGATCTGTTCGGCCACtt                                                                                               >  6:23282/1‑112 (MQ=255)
                                                                                           gATGGTTACCAGCAGCTCATGGCTGCCTGCCAGCTCTAGCACCAGCGCCTCGTCCAGCGGCTTGACGAAGCGCATGTCGACCACCGTGGCGTTGATCTGTTCGGCCACTTGCATG‑GCCTCGGCCAGTTGTACGCCGAACACCAGCAggg                                                       <  8:290568/149‑1 (MQ=255)
                                                                                           gATGGTTACCAGCAGCTCATGGCTGCCTGCCAGCTCTAGCACCAGCGCCTCGTCCAGCGGCTTGACGAAGCGCATGTCGACCACCGTGGCGTTGATCTGTTCGGCCACTTGCATG‑GCCTCGGCCAGTTGTACGCCGAACACCAGCAgg                                                        <  1:46674/148‑1 (MQ=255)
                                                                                                  aCCAGCAGCTCATGGCTGCCTGCCAGCTCTAGCACCAGCGCCTCGTCCAGCGGCTTGACGAAGCGCATGTCGACCACCGTGGCGTTGATCTGTTCGGCCACTTGCATG‑GCCTCGGCCAGTTg                                                                           >  4:298806/1‑122 (MQ=255)
                                                                                                   ccAGCAGCTCATGGCTGCCTGCCAGCTCTAGCACCAGCGCCTCGTCCAGCGGCTTGACGAAGCGCATGTCGACCACCGTGGCGTTGATCTGTTCGGCCACTTGCATG‑GCCTCGGCCAGTTGTACGCCGAACACCAGCAGGGCCACttt                                                <  4:7552/148‑1 (MQ=255)
                                                                                                       cagcTCATGGCTGCCTGCCAGCTCTAGCACCAGCGCCTCGTCCAGCGGCTTGACGAAGCGCATGTCGACCACCGTGGCGTTGATCTGTTCGGCCACTTGCATGCGCCTCGGCCAGTTGTACGCCGAACACCAGCAGGGCCACTTTTTCg                                            <  1:257625/149‑1 (MQ=255)
                                                                                                             aTGGCTGCCTGCCAGCTCTAGCACCAGCGCCTCGTCCAGCGGCTTGACGAAGCGCATGTCGACCACCGTGGCGTTGATCTGTTCTGCCAGTTGCATG‑GCCTCGGCCAGTTGTACGCCGAACACCAGCAGGGCCACTTTTTCGCCCTg                                       <  6:210539/147‑1 (MQ=255)
                                                                                                             aTGGCTGCCTGCCAGCTCTAGCACCAGCGCCTCGTCCAGCGGCTTGACGAAGCGCATGTCGACCACCGTGGCGTTGATCTGTTCGGCCACTTGCATG‑GCCTCGGCCAGTTGTACGCCGAACACCAGCAGGGc                                                      <  1:171590/132‑1 (MQ=255)
                                                                                                             aTGGCTGCCTGCCAGCTCTAGCACCAGCGCCTCGTCCAGCGGCTTGACGAAGCGCATGTCGACCACCGTGGCGTTGATCTGTTCGGCCACTTGCATG‑GCCTCGGCCAGTTGTACGCCGAACACCAGCAGGGc                                                      >  2:171590/1‑132 (MQ=255)
                                                                                                             aTGGCTGCCTGCCAGCTCTAGCACCAGCGCCTCGTCCAGCGGCTTGACGAAGCGCATGTCGACCACCGTGGCGTTGATCTGTTCGGCCACTTGCATG‑GCCTCGGCCAGTTGTACGCCGAACACCAGCAGGGCCAc                                                   >  4:253566/1‑135 (MQ=255)
                                                                                                             aTGGCTGCCTGCCAGCTCTAGCACCAGCGCCTCGTCCAGCGGCTTGACGAAGCGCATGTCGACCACCGTGGCGTTGATCTGTTCGGCCACTTGCATG‑GCCTCGGCCAGTTGTACGCCGAACACCAGCAGGGCCAc                                                   <  3:253566/135‑1 (MQ=255)
                                                                                                             aTGGCTGCCTGCCAGCTCTAGCACCAGCGCCTCGTCCAGCGGCTTGACGAAGCGCATGTCGACCACCGTGGCGTTGATCTGTTCGGCCACTTGCATG‑GCCTCGGCCAGTTGTACGCCGAACACCAGCAGGGCCACTTTTTCGCCCTgg                                      <  5:182738/148‑1 (MQ=255)
                                                                                                                 ctgcctgcCAGCTCTAGCACCAGCGCCTCGTCCAGCGGCTTGACGAAGCGCATGTCGACCACCGTGGCGTTGATCTGTACGGCCACTTGCATG‑GCCTCGGCCAGTTGTACGCCGAACACCAGCAGGGCCACTTTTTCGCCCTGgcggcg                                 <  4:192924/149‑1 (MQ=255)
                                                                                                                  tgcctgccAGCTCTAGCACCAGCGCCTCGTCCAGCGGCTTGACGAAGCGCATGTCGACCACCGTGGCGTTGATCTGTTCGGCCACTTGCATG‑GCCTCGGCCAGTTGTACGCCGAACACCAGCAGGGCCACTTTTTCGCCCTGGcggcgg                                >  3:264161/1‑149 (MQ=255)
                                                                                                                   gcctgccAGCTCTAGCACCAGCGCCTCGTCCAGCGGCTTGACGAAGCGCATGTCGACCACCGTGGCGTTGATCTGTTCGGCCACTTGCATG‑GCCTCGGCCAGTTGTACGCCGAACACCAGCAGGGCCACTTTTTCGCCCTGGCGGCGGa                               >  7:75638/1‑149 (MQ=255)
                                                                                                                        ccAGCTCTAGCACCAGCGCCTCGTCCAGCGGCTTGACGAAGCGCATGTCGACCACCGTGGCGTTGATCTGTTCGGCCACTTGCATG‑GCCTCGGCCAGTTGTACGCCGAACACCAGCAGGGCCACTTTTTCGCCCTGGCGGCGGACCACg                          >  1:208237/1‑149 (MQ=255)
                                                                                                                        ccAGCTCTAGCACCAGCGCCTCGTCCAGCGGCTTGACGAAGCGCATGTCGACCACCGTGGCGTTGATCTGTTCGGCCACTTGCATG‑GCCTCGGCCAGTTGTACGCCGAACACCAGCAGGGCCACTTTTTCGCCCTGGCGGCGGACCACg                          >  2:114395/1‑149 (MQ=255)
                                                                                                                            ctctAGCACCAGCGCCTCGTCCAGCGGCTTGACGAAGCGCATGTCGACCACCGTGGCGTTGATCTGTTCGGCCACTTGCATG‑GCCTCGGCCAGTTGTACGCCGAACACCAGCAGGGCCACTTTTTCGCCCTGGCGGCGGACCACGCCCt                      >  8:279241/1‑149 (MQ=255)
                                                                                                                               tAGCACCAGCGCCTCGTCCAGCGGCTTGACGAAGCGCATGTCGACCACCGTGGCGTTGATCTGTTCGGCCACTTGCATG‑GCCTCGGCCAGTTGTACGCCGAACACCAGCAGGGCCACTTTTTCGCCCTGGCGGCGGACCACGCCCTTGc                   >  3:114302/1‑149 (MQ=255)
                                                                                                                                   aCCAGCGCCTCGTCCAGCGGCTTGACGAAGCGCATGTCGACCACCGTGGCGTTGATCTGTTCGGCCACTTGCATG‑GCCTCGGCCAGTTGTACGCCGAACACCAGCAGGGCCACTTTTTCGCCCTGGCGGCGGACCACGCCCTTGCCGAt               >  6:353714/1‑149 (MQ=255)
                                                                                                                                    ccAGCGCCTCGTCCAGCGGCTTGACGAAGCGCATGTCGACCACCGTGGCGTTGATCTGTTCGGCCACTTGCATG‑GCCTCGGCCAGTTGTACGCCGAACACCAGCAGGGCCACTTTTTCGCCCTGGCGGCGGACCACGCCCTTGCCGAtc              >  3:146058/1‑149 (MQ=255)
                                                                                                                                    catgcgcTTCGTCAAGCCGCTGGACGAGGCGCATGTCGACCACCGTGGCGTTGATCTGTTCGGCCACTTGCATG‑GCCTCGGCCAGTTGTACGCCGAACACCAGCAGGGCCACTTTTTCGCCCTGGCGGCGGACCACGcc                        >  7:98574/4‑139 (MQ=255)
                                                                                                                                    catgcgcTTCGTCAAGCCGCTGGACGAGGCGCATGTCGACCACCGTGGCGTTGATCTGTTCGGCCACTTGCATG‑GCCTCGGCCAGTTGTACGCCGAACACCAGCAGGGCCACTTTTTCGCCCTGGCGGCGGACCACGcc                        <  8:98574/136‑1 (MQ=255)
                                                                                                                                    catgcgcTTCGTCAAGCCGCTGGACGAGGCGCATGTCGACCACCGTGGCGTTGATCTGTTCGGCCACTTGCATG‑GCCTCGGCCAGTTGTACGCCGAACACCAGCAGGGCCACTTTTTCGCCCTGGCGGCGGACCACGCCCTTGCCGAtc              >  7:333188/4‑149 (MQ=255)
                                                                                                                                    catgcgcTTCGTCAAGCCGCTGGACGAGGCGCATGTCGACCACCGTGGCGTTGATCTGTTAGGCCACTTGCATG‑GCCTCGGCCAGTTGTACGCCGAACACCAGCAGGGCCACTTTTTCGCCCTGGCGGCGGACCACGCCCTTGCCGAtc              >  5:270907/4‑149 (MQ=255)
                                                                                                                                     cAGCGCCTCGTCCAGCGGCTTGACGAAGCGCATGTCGACCACCGTGGCGTTGATCTGTTCGGc                                                                                                    >  2:34387/1‑63 (MQ=255)
                                                                                                                                     cAGCGCCTCGTCCAGCGGCTTGACGAAGCGCATGTCGACCACCGTGGCGTTGATCTGTTCGGc                                                                                                    <  1:34387/63‑1 (MQ=255)
                                                                                                                                     cAGCGCCTCGTCCAGCGGCTTGACGAAGCGCATGTCGACCACCGTGGCGTTGATCTGTTCGGCCACTTGCATG‑GCCTCGGCCAGTTGTACGCCGAACACCAGCAGGGc                                                      >  6:2748/1‑108 (MQ=255)
                                                                                                                                     cAGCGCCTCGTCCAGCGGCTTGACGAAGCGCATGTCGACCACCGTGGCGTTGATCTGTTCGGCCACTTGCATG‑GCCTCGGCCAGTTGTACGCCGAACACCAGCAGGGc                                                      <  5:2748/108‑1 (MQ=255)
                                                                                                                                       gcgcCTCGTCCAGCGGCTTGACGAAGCGCATGTCGACCACCGTGGCGTTGATCTGTTCGGCCACTTGCATG‑GCCTCGGCCAGTTGTACGCCGAACACCAGCAGGGCCACTTTTTCGCCCTGGCGGCGGACCACGCCCTTGCCGAtctc            >  8:281000/1‑148 (MQ=255)
                                                                                                                                           cTCGTCCAGCGGCTTGACGAAGCGCATGTCGACCACCGTGGCGTTGATCTGTTCGGCCACTTGCATG‑GCCTCGGCCAGTTGTACGCCGAACACCAGCAGGGCCACTTTTTCGCCCTGGCGGCGGACCACGCCCTTGCCGATCTCCAGTg       <  8:75638/149‑1 (MQ=255)
                                                                                                                                                 cAGCGGCTTGACGAAGCGCATGTCGACCACCGTGGCGTTGATCTGTTCGGCCACTTGCATG‑GCCTCGGCCAGTTGTACGCCGAACACCAGCAGGGCCACTTTTTCGCCCTGGCGGCGGACCACGCCCTTGCCGATCTCCAGTGGTTGc  <  7:124069/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                    
GCCCCAGGTGCAGAAGCGGCTTGAGCACTGCCTGGCTGGCAAGGAACTCACCCACCGCAGCGCCCGCGCCACCCATGATGGCGTTCTCTTCGATGGTTACCAGCAGCTCATGGCTGCCTGCCAGCTCTAGCACCAGCGCCTCGTCCAGCGGCTTGACGAAGCGCATGTCGACCACCGTGGCGTTGATCTGTTCGGCCACTTGCATG‑GCCTCGGCCAGTTGTACGCCGAACACCAGCAGGGCCACTTTTTCGCCCTGGCGGCGGACCACGCCCTTGCCGATCTCCAGTGGTTGC  >  NC_002947/612402‑612694

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑