Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	612,551	G→C	9.7%	P544R (CCG→CGG)	dxs ←	1‑deoxyxylulose‑5‑phosphate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	612,551	0	G	C	9.7%	178.8 / 10.0	62	P544R (CCG→CGG)	dxs	1‑deoxyxylulose‑5‑phosphate synthase
Reads supporting (aligned to +/- strand):  ref base G (29/27);  new base C (3/3);  total (32/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.75e-01

CCCAGGTGCAGAAGCGGCTTGAGCACTGCCTGGCTGGCAAGGAACTCACCCACCGCAGCGCCCGCGCCACCCATGATGGCGTTCTCTTCGATGGTTACCAGCAGCTCATGGCTGCCTGCCAGCTCTAGCACCAGCGCCTCGTCCAGCGGCTTGACGAAGCGCATGTCGACCACCGTGGCGTTGATCTGTTCGGCCACTTGCATG‑GCCTCGGCCAGTTGTACGCCGAACACCAGCAGGGCCACTTTTTCGCCCTGGCGGCGGACCACGCCCTTGCCGATCTCCAGTGGTTGCAG  >  NC_002947/612404‑612696                                                                                                                                                    |                                                                                                                                                   cccAGGTGCAGAAGCGGCTTGAGCACTGCCTGGCTGGCAAGGAACTCACCCACCGCAGCGCCCGCGCCACCCATGATGGCGTTCTCTTCGATGGTTACCAGCAGCTCATGGCTGCCTGCCAGCTCTAGCACCAGCGCCTCGTCCAGCgg                                                                                                                                                   >  3:192924/1‑149 (MQ=255)  ccAGGTGCAGAAGCGGCTTGAGCACTGCCTGGCTGGCAAGGAACTCACCCACCGCAGCGCCCGCGCCACCCATGATGGCGTTCTCTTCGATGGTTACCAGCAGCTCATGGCTGCCTGCCAGCTCTAGCACCAGCGCCTCGTCCAGCGGc                                                                                                                                                  >  6:52437/1‑149 (MQ=255)          aGAAGCGGCTTGAGCACTGCCTGGCTGGCAAGGAACTCACCCACCGCAGCGCCCGCGCCACCCATGATGGCGTTCTCTTCGATGGTTACCAGCAGCTCATGGCTGCCTGCCAGCTCTAGCACCAGCGCCTCGTCCAGCGGCTTGACGaa                                                                                                                                          <  7:67404/149‑1 (MQ=255)           gAAGCGGCTTGAGCACTGCCTGGCTGGCAAGGAACTCACCCACCGCAGCGCCCGCGCCACCCATGATGGCGTTCTCTTCGATGGTTACCAGCAGCTCATGGCTGCCTGCCAGCTCTAGCACCAGCGCCTCGTCCAGCGGCTTGACGAAg                                                                                                                                         <  3:353584/149‑1 (MQ=255)                  cTTGAGCACTGCCTGGCTGGCAAGGAACTCACCCACCGCAGCGCCCGCGCCACCCATGATGGCGTTCTCTTCGATGGTTACCAGCAGCTCATGGCTGCCTGCCAGCTCTAGCACCAGCGCTTCGTCAAGCCGCTGGACGAGgcgctggt                                                                                                                                  <  5:146150/149‑5 (MQ=255)                   ttGAGCACTGCCTGGCTGGCAAGGAACTCACCCACCGCAGCGCCCGCGCCACCCATGATGGCGTTCTCTTCGATGGTTACCAGCAGCTCATGGCTGCCTGCCAGCTCTAGCACCAGCGCCTCGTCCAGCGGCTTGACGAAGCGCATGTc                                                                                                                                 >  4:338229/1‑149 (MQ=255)                     gAGCACTGCCTGGCTGGCAAGGAACTCACCCACCGCAGCGCCCGCGCCACCCATGATGGCGTTCTCTTCGATGGTTACCAGCAGCTCATGGCTGCCTGCCAGCTCTAGCACCAGCGCTTCGTCAAGCCGCTGGACGAGgcgctggtg                                                                                                                                 <  7:166008/147‑6 (MQ=255)                                       aaGGAACTCACCCACCGCAGCGCCCGCGCCACCCATGATGGCGTTCTCTTCGATGGTTACCAGCAGCTCATGGCTGCCTTCCAGCTCTAGCACCAGCGCCTCGTCCAGCGGCTTGACGAAGCGCATGTCGACCACCGTGGCGTTGATCt                                                                                                             <  4:180789/149‑1 (MQ=255)                                              tCACCCACCGCAGCGCCCGCGCCACCCATGATGGCGTTCTCTTCGATGGTTACCAGCAGCTCATGGCTGCCTGCCAGCTCTAGCACCAGCGCCTCGTCCAGCGGCTTGACGAAGCGCATGTCGACCACCGTGGCGTTGATCTGTTCGGc                                                                                                      >  2:257625/1‑149 (MQ=255)                                               cacccaccGCAGCGCCCGCGCCACCCATGATGGCGTTCTCTTCGATGGTTACCAGCAGCTCATGGCTGCCTGCCAGCTCTAGCACCAGCGCCTCGTCCAGCGGCTTGACGAAGCGCATGTCGACCACCGTGGCGTTGATCTGTTCGGc                                                                                                      >  1:163142/1‑148 (MQ=255)                                                acccaccGCAGCGCCCGCGCCACCCATGATGGCGTTCTCTTCGATGGTTACCAGCAGCTCATGGCTGCCTGCCAGCTCTAGCACCAGCGCCTCGTCCAGCGGCTTGACGAAGCGCATGTCGACCACCGTGGCGTTGATCTGTTCGGCCa                                                                                                    >  3:321211/1‑149 (MQ=255)                                                 cccaccGCAGCGCCCGCGCCACCCATGATGGCGTTCTCTTCGATGGTTACCAGCAGCTCATGGCTGCCTGCCAGCTCTAGCACCAGCGCCTCGTCCAGCGGCTTGACGAAGCGCATGTCGACCACCGTGGCGTTGATCTGTTCGGCCAc                                                                                                   >  3:152354/1‑149 (MQ=255)                                                       gCAGCGCCCGCGCCACCCATGATGGCGTTCTCTTCGATGGTTACCAGCAGCTCATGGCTGCCTGCCAGCTCTAGCACCAGCGCCTCGTCCAGCGGCTTGACGAAGCGCATGTCGACCACCGTGGCGTTGATCTGTTCGGCCACTTGCAt                                                                                             <  4:152354/149‑1 (MQ=255)                                                        cAGCGCCCGCGCCACCCATGATGGCGTTCTCTTCGATGGTTACCAGCAGCTCATGGCTGCCTGCCAGCTCTAGCACCAGCGCCTCGTCCAGCGGCTTGACGAAGCGCATGTCGacca                                                                                                                            <  7:123989/117‑1 (MQ=255)                                                        cAGCGCCCGCGCCACCCATGATGGCGTTCTCTTCGATGGTTACCAGCAGCTCATGGCTGCCTGCCAGCTCTAGCACCAGCGCCTCGTCCAGCGGCTTGACGAAGCGCATGTCGacca                                                                                                                            >  8:123989/1‑117 (MQ=255)                                                        cAGCGCCCGCGCCACCCATGATGGCGTTCTCTTCGATGGTTACCAGCAGCTCATGGCTGCCTGCCAGCTCTAGCACCAGCGCCTCGTCCAGCGGCTTGACGAAGCGCATGTCGACCACCGTGGCGTTGATCTGTTCGGCCACTTGCATg                                                                                            <  4:321211/149‑1 (MQ=255)                                                                    cACCCATGATGGCGTTCTCTTCGATGGTTACCAGCAGCTCATGGCTGCCTGCCAGCTCTAGCACCACCGCCTCGTCCAGCGGCTTGACGAAGCGCATGTCGACCACCGTGGCGTTGATCTGTTCGGCCACTTGCATG‑GCCTCGGCCAGt                                                                               >  8:124069/1‑149 (MQ=255)                                                                     aCCCATGATGGCGTTCTCTTCGATGGTTACCAGCAGCTCATGGCTGCCTGCCAGCTCTAGCACCAGCGCCTCGTCCAGCGGCTTGACGAAGCGCATGTCGACCACCGTGGCGTTGATCTGTTCGGCCACTTGCATG‑GCCTCGGCCAGtt                                                                              >  5:291361/1‑149 (MQ=255)                                                                     aCCCATGATGGCGTTCTCTTCGATGGTTACCAGCAGCTCATGGCTGCCTGCCAGCTCTAGCACCAGCGCCTCGTCCAGCGGCTTGACGAAGCGCATGTCGACCACCGTGGCGTTGATCTGTTCGGCCACTTGCATG‑GCCTCGGCCAGtt                                                                              <  5:52437/149‑1 (MQ=255)                                                                         atgatgGCGTTCTCTTCGATGGTTACCAGCAGCTCATGGCTGCCTGCGAGCTCTAGCTCCAGCGCCTCGTCCAGCGGCTTGACGAAGCGCATGTCGTCCACGGTGGCGTTGATCTGTTCGGCCACTTGCATG‑GCCTCGGCCAGTTGTAc                                                                          <  5:112177/149‑1 (MQ=255)                                                                         atgatgGCGTTCTCTTCGATGGTTACCAGCAGCTCATGGCTGCCTGCCAGCTCTAGCACCAGCGCCTCGTCCAGCGGCTTGACGAAGCGCATGTCGACCACCGTGGCGTTGATCTGTTCGGCCACTTGCATG‑GCCTCGGCCAGTTGTAc                                                                          <  3:338229/149‑1 (MQ=255)                                                                         atgatgGCGTTCTCTTCGATGGTTACCAGCAGCTCATGGCTGCCTGCCAGCTCTAGCACCAGCGCCTCGTCCAGCGGCTTGACGAAGCGCATGTCGACCACCGTGGCGTTGATCTGTTCGGCCACTTGCATG‑GCCTCGGCCAGTTGTAc                                                                          >  6:182738/1‑149 (MQ=255)                                                                           gatgGCGTTCTCTTCGATGGTTACCAGCAGCTCATGGCTGCCTGCCAGCTCTAGCACCAGCGCCTCGTCCAGCGGCTTGACGAAGCGCATGTCGACCACCGTGGCGTTGATCTGTTCGGCCACTTGCATG‑GCCTCGGc                                                                                   <  6:264670/138‑1 (MQ=255)                                                                           gatgGCGTTCTCTTCGATGGTTACCAGCAGCTCATGGCTGCCTGCCAGCTCTAGCACCAGCGCCTCGTCCAGCGGCTTGACGAAGCGCATGTCGACCACCGTGGCGTTGATCTGTTCGGCCACTTGCATG‑GCCTCGGc                                                                                   >  5:264670/1‑138 (MQ=255)                                                                                  ttCTCTTCGATGGTTACCAGCAGCTCATGGCTGCCTGCCAGCTCTAGCACCAGCGCCTCGTCCAGCGGCTTGACGAAGCGCATGTCGACCACCGTGGCGTTGATCTGTTCGGCCACTTGCATG‑GCCTCGGCCAGTTGTACGCCGAacac                                                                 <  4:154820/149‑1 (MQ=255)                                                                                    ctctTCGATGGTTACCAGCAGCTCATGGCTGCCTGCCAGCTCTAGCACCAGCGCCTCGTCCAGCGGCTTGACGAAGCGCATGTCGACCACCGTGGCGTTGATCTGTTCGGCCACTTGCATG‑GCCTCGGCCAGTTGTACGCCGAacac                                                                 >  4:250416/1‑147 (MQ=255)                                                                                    ctctTCGATGGTTACCAGCAGCTCATGGCTGCCTGCCAGCTCTAGCACCAGCGCCTCGTCCAGCGGCTTGACGAAGCGCATGTCGACCACCGTGGCGTTGATCTGTTCGGCCACTTGCATG‑GCCTCGGCCAGTTGTACGCCGAACAcc                                                                <  2:163142/148‑1 (MQ=255)                                                                                     tctTCGATGGTTACCAGCAGCTCATGGCTGCCTGCCAGCTCTAGCACCAGCGCCTCGTCCAGCGGCTTGACGAAGCGCATGTCGACCACCGTGGCGTTGATCTGTTCGGCCACTTGCATG‑GCCTCGGCCAGTTGTACGCCGAACACcag                                                              >  5:210539/1‑149 (MQ=255)                                                                                       ttCGATGGTTACCAGCAGCTCATGGCTGCCTGCCAGCTCTAGCACCAGCGCCTCGTCCAGCGGCTTGACGAAGCGCATGTCGACCACCGTGGCGTTGATCTGTTCGGCCACTTGCATG‑GCCTCGGCCAGTTGTACGCCGAACACcag                                                              >  7:290568/1‑147 (MQ=255)                                                                                        tCGATGGTTACCAGCAGCTCATGGCTGCCTGCCAGCTCTAGCACCAGCGCCTCGTCCAGCGGCTTGACGAAGCGCATGTCGACCACCGTGGCGTTGATCTGTTCGGCCACtt                                                                                                 >  6:23282/1‑112 (MQ=255)                                                                                        tCGATGGTTACCAGCAGCTCATGGCTGCCTGCCAGCTCTAGCACCAGCGCCTCGTCCAGCGGCTTGACGAAGCGCATGTCGACCACCGTGGCGTTGATCTGTTCGGCCACtt                                                                                                 <  5:23282/112‑1 (MQ=255)                                                                                          gATGGTTACCAGCAGCTCATGGCTGCCTGCCAGCTCTAGCACCAGCGCCTCGTCCAGCGGCTTGACGAAGCGCATGTCGACCACCGTGGCGTTGATCTGTTCGGCCACTTGCATG‑GCCTCGGCCAGTTGTACGCCGAACACCAGCAggg                                                         <  8:290568/149‑1 (MQ=255)                                                                                          gATGGTTACCAGCAGCTCATGGCTGCCTGCCAGCTCTAGCACCAGCGCCTCGTCCAGCGGCTTGACGAAGCGCATGTCGACCACCGTGGCGTTGATCTGTTCGGCCACTTGCATG‑GCCTCGGCCAGTTGTACGCCGAACACCAGCAgg                                                          <  1:46674/148‑1 (MQ=255)                                                                                                 aCCAGCAGCTCATGGCTGCCTGCCAGCTCTAGCACCAGCGCCTCGTCCAGCGGCTTGACGAAGCGCATGTCGACCACCGTGGCGTTGATCTGTTCGGCCACTTGCATG‑GCCTCGGCCAGTTg                                                                             >  4:298806/1‑122 (MQ=255)                                                                                                  ccAGCAGCTCATGGCTGCCTGCCAGCTCTAGCACCAGCGCCTCGTCCAGCGGCTTGACGAAGCGCATGTCGACCACCGTGGCGTTGATCTGTTCGGCCACTTGCATG‑GCCTCGGCCAGTTGTACGCCGAACACCAGCAGGGCCACttt                                                  <  4:7552/148‑1 (MQ=255)                                                                                                      cagcTCATGGCTGCCTGCCAGCTCTAGCACCAGCGCCTCGTCCAGCGGCTTGACGAAGCGCATGTCGACCACCGTGGCGTTGATCTGTTCGGCCACTTGCATGCGCCTCGGCCAGTTGTACGCCGAACACCAGCAGGGCCACTTTTTCg                                              <  1:257625/149‑1 (MQ=255)                                                                                                            aTGGCTGCCTGCCAGCTCTAGCACCAGCGCCTCGTCCAGCGGCTTGACGAAGCGCATGTCGACCACCGTGGCGTTGATCTGTTCTGCCAGTTGCATG‑GCCTCGGCCAGTTGTACGCCGAACACCAGCAGGGCCACTTTTTCGCCCTg                                         <  6:210539/147‑1 (MQ=255)                                                                                                            aTGGCTGCCTGCCAGCTCTAGCACCAGCGCCTCGTCCAGCGGCTTGACGAAGCGCATGTCGACCACCGTGGCGTTGATCTGTTCGGCCACTTGCATG‑GCCTCGGCCAGTTGTACGCCGAACACCAGCAGGGc                                                        <  1:171590/132‑1 (MQ=255)                                                                                                            aTGGCTGCCTGCCAGCTCTAGCACCAGCGCCTCGTCCAGCGGCTTGACGAAGCGCATGTCGACCACCGTGGCGTTGATCTGTTCGGCCACTTGCATG‑GCCTCGGCCAGTTGTACGCCGAACACCAGCAGGGc                                                        >  2:171590/1‑132 (MQ=255)                                                                                                            aTGGCTGCCTGCCAGCTCTAGCACCAGCGCCTCGTCCAGCGGCTTGACGAAGCGCATGTCGACCACCGTGGCGTTGATCTGTTCGGCCACTTGCATG‑GCCTCGGCCAGTTGTACGCCGAACACCAGCAGGGCCAc                                                     >  4:253566/1‑135 (MQ=255)                                                                                                            aTGGCTGCCTGCCAGCTCTAGCACCAGCGCCTCGTCCAGCGGCTTGACGAAGCGCATGTCGACCACCGTGGCGTTGATCTGTTCGGCCACTTGCATG‑GCCTCGGCCAGTTGTACGCCGAACACCAGCAGGGCCAc                                                     <  3:253566/135‑1 (MQ=255)                                                                                                            aTGGCTGCCTGCCAGCTCTAGCACCAGCGCCTCGTCCAGCGGCTTGACGAAGCGCATGTCGACCACCGTGGCGTTGATCTGTTCGGCCACTTGCATG‑GCCTCGGCCAGTTGTACGCCGAACACCAGCAGGGCCACTTTTTCGCCCTgg                                        <  5:182738/148‑1 (MQ=255)                                                                                                                ctgcctgcCAGCTCTAGCACCAGCGCCTCGTCCAGCGGCTTGACGAAGCGCATGTCGACCACCGTGGCGTTGATCTGTACGGCCACTTGCATG‑GCCTCGGCCAGTTGTACGCCGAACACCAGCAGGGCCACTTTTTCGCCCTGgcggcg                                   <  4:192924/149‑1 (MQ=255)                                                                                                                 tgcctgccAGCTCTAGCACCAGCGCCTCGTCCAGCGGCTTGACGAAGCGCATGTCGACCACCGTGGCGTTGATCTGTTCGGCCACTTGCATG‑GCCTCGGCCAGTTGTACGCCGAACACCAGCAGGGCCACTTTTTCGCCCTGGcggcgg                                  >  3:264161/1‑149 (MQ=255)                                                                                                                  gcctgccAGCTCTAGCACCAGCGCCTCGTCCAGCGGCTTGACGAAGCGCATGTCGACCACCGTGGCGTTGATCTGTTCGGCCACTTGCATG‑GCCTCGGCCAGTTGTACGCCGAACACCAGCAGGGCCACTTTTTCGCCCTGGCGGCGGa                                 >  7:75638/1‑149 (MQ=255)                                                                                                                       ccAGCTCTAGCACCAGCGCCTCGTCCAGCGGCTTGACGAAGCGCATGTCGACCACCGTGGCGTTGATCTGTTCGGCCACTTGCATG‑GCCTCGGCCAGTTGTACGCCGAACACCAGCAGGGCCACTTTTTCGCCCTGGCGGCGGACCACg                            >  1:208237/1‑149 (MQ=255)                                                                                                                       ccAGCTCTAGCACCAGCGCCTCGTCCAGCGGCTTGACGAAGCGCATGTCGACCACCGTGGCGTTGATCTGTTCGGCCACTTGCATG‑GCCTCGGCCAGTTGTACGCCGAACACCAGCAGGGCCACTTTTTCGCCCTGGCGGCGGACCACg                            >  2:114395/1‑149 (MQ=255)                                                                                                                           ctctAGCACCAGCGCCTCGTCCAGCGGCTTGACGAAGCGCATGTCGACCACCGTGGCGTTGATCTGTTCGGCCACTTGCATG‑GCCTCGGCCAGTTGTACGCCGAACACCAGCAGGGCCACTTTTTCGCCCTGGCGGCGGACCACGCCCt                        >  8:279241/1‑149 (MQ=255)                                                                                                                              tAGCACCAGCGCCTCGTCCAGCGGCTTGACGAAGCGCATGTCGACCACCGTGGCGTTGATCTGTTCGGCCACTTGCATG‑GCCTCGGCCAGTTGTACGCCGAACACCAGCAGGGCCACTTTTTCGCCCTGGCGGCGGACCACGCCCTTGc                     >  3:114302/1‑149 (MQ=255)                                                                                                                                  aCCAGCGCCTCGTCCAGCGGCTTGACGAAGCGCATGTCGACCACCGTGGCGTTGATCTGTTCGGCCACTTGCATG‑GCCTCGGCCAGTTGTACGCCGAACACCAGCAGGGCCACTTTTTCGCCCTGGCGGCGGACCACGCCCTTGCCGAt                 >  6:353714/1‑149 (MQ=255)                                                                                                                                   ccAGCGCCTCGTCCAGCGGCTTGACGAAGCGCATGTCGACCACCGTGGCGTTGATCTGTTCGGCCACTTGCATG‑GCCTCGGCCAGTTGTACGCCGAACACCAGCAGGGCCACTTTTTCGCCCTGGCGGCGGACCACGCCCTTGCCGAtc                >  3:146058/1‑149 (MQ=255)                                                                                                                                   catgcgcTTCGTCAAGCCGCTGGACGAGGCGCATGTCGACCACCGTGGCGTTGATCTGTTCGGCCACTTGCATG‑GCCTCGGCCAGTTGTACGCCGAACACCAGCAGGGCCACTTTTTCGCCCTGGCGGCGGACCACGcc                          >  7:98574/4‑139 (MQ=255)                                                                                                                                   catgcgcTTCGTCAAGCCGCTGGACGAGGCGCATGTCGACCACCGTGGCGTTGATCTGTTCGGCCACTTGCATG‑GCCTCGGCCAGTTGTACGCCGAACACCAGCAGGGCCACTTTTTCGCCCTGGCGGCGGACCACGcc                          <  8:98574/136‑1 (MQ=255)                                                                                                                                   catgcgcTTCGTCAAGCCGCTGGACGAGGCGCATGTCGACCACCGTGGCGTTGATCTGTTCGGCCACTTGCATG‑GCCTCGGCCAGTTGTACGCCGAACACCAGCAGGGCCACTTTTTCGCCCTGGCGGCGGACCACGCCCTTGCCGAtc                >  7:333188/4‑149 (MQ=255)                                                                                                                                   catgcgcTTCGTCAAGCCGCTGGACGAGGCGCATGTCGACCACCGTGGCGTTGATCTGTTAGGCCACTTGCATG‑GCCTCGGCCAGTTGTACGCCGAACACCAGCAGGGCCACTTTTTCGCCCTGGCGGCGGACCACGCCCTTGCCGAtc                >  5:270907/4‑149 (MQ=255)                                                                                                                                    cAGCGCCTCGTCCAGCGGCTTGACGAAGCGCATGTCGACCACCGTGGCGTTGATCTGTTCGGc                                                                                                      >  2:34387/1‑63 (MQ=255)                                                                                                                                    cAGCGCCTCGTCCAGCGGCTTGACGAAGCGCATGTCGACCACCGTGGCGTTGATCTGTTCGGc                                                                                                      <  1:34387/63‑1 (MQ=255)                                                                                                                                    cAGCGCCTCGTCCAGCGGCTTGACGAAGCGCATGTCGACCACCGTGGCGTTGATCTGTTCGGCCACTTGCATG‑GCCTCGGCCAGTTGTACGCCGAACACCAGCAGGGc                                                        >  6:2748/1‑108 (MQ=255)                                                                                                                                    cAGCGCCTCGTCCAGCGGCTTGACGAAGCGCATGTCGACCACCGTGGCGTTGATCTGTTCGGCCACTTGCATG‑GCCTCGGCCAGTTGTACGCCGAACACCAGCAGGGc                                                        <  5:2748/108‑1 (MQ=255)                                                                                                                                      gcgcCTCGTCCAGCGGCTTGACGAAGCGCATGTCGACCACCGTGGCGTTGATCTGTTCGGCCACTTGCATG‑GCCTCGGCCAGTTGTACGCCGAACACCAGCAGGGCCACTTTTTCGCCCTGGCGGCGGACCACGCCCTTGCCGAtctc              >  8:281000/1‑148 (MQ=255)                                                                                                                                          cTCGTCCAGCGGCTTGACGAAGCGCATGTCGACCACCGTGGCGTTGATCTGTTCGGCCACTTGCATG‑GCCTCGGCCAGTTGTACGCCGAACACCAGCAGGGCCACTTTTTCGCCCTGGCGGCGGACCACGCCCTTGCCGATCTCCAGTg         <  8:75638/149‑1 (MQ=255)                                                                                                                                                cAGCGGCTTGACGAAGCGCATGTCGACCACCGTGGCGTTGATCTGTTCGGCCACTTGCATG‑GCCTCGGCCAGTTGTACGCCGAACACCAGCAGGGCCACTTTTTCGCCCTGGCGGCGGACCACGCCCTTGCCGATCTCCAGTGGTTGc    <  7:124069/148‑1 (MQ=255)                                                                                                                                                 aGCGGCTTGACGAAGCGCATGTCGACCACCGTGGCGTTGATCTGTTCGGCCACTTGCATG‑GCCTCGGCCAGTTGTACGCCGAACACCAGCAGGGCCACTTTTTCGCCCTGGCGGCGGACCACGCCCTTGCCGATCTCCAGTGGTTGCAg  <  7:281000/149‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                   CCCAGGTGCAGAAGCGGCTTGAGCACTGCCTGGCTGGCAAGGAACTCACCCACCGCAGCGCCCGCGCCACCCATGATGGCGTTCTCTTCGATGGTTACCAGCAGCTCATGGCTGCCTGCCAGCTCTAGCACCAGCGCCTCGTCCAGCGGCTTGACGAAGCGCATGTCGACCACCGTGGCGTTGATCTGTTCGGCCACTTGCATG‑GCCTCGGCCAGTTGTACGCCGAACACCAGCAGGGCCACTTTTTCGCCCTGGCGGCGGACCACGCCCTTGCCGATCTCCAGTGGTTGCAG  >  NC_002947/612404‑612696

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑