Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	648,289	A→T	7.9%	intergenic (+58/+33)	acoR → / ← accC	acetoin catabolism regulatory protein/acetyl‑CoA carboxylase biotin carboxylase subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	648,289	0	A	T	7.9%	126.2 / 3.1	51	intergenic (+58/+33)	acoR/accC	acetoin catabolism regulatory protein/acetyl‑CoA carboxylase biotin carboxylase subunit
Reads supporting (aligned to +/- strand):  ref base A (22/25);  new base T (2/2);  total (24/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.81e-01

ATGCGGCACGGGCTTTGGGCATTTCGCGGGCGACGCTTTATCGGCGAGTGCATGAGCACCGCATCGACATGCCGCGGATGAAGGGGTAGAGCCCTGGGGCCGCGACGCGGCCCCAGATTCTGGCAG‑GCAATAAAAAACCCCGGCACAGGG‑CCGGGGTTCTGGTACATCACACTACACGATCACTCCTGGTTGGCCAGTTTGTGCTCGAGGTAGTGAATGTTGACGCCGCCTTTGCAGAAG‑CCTTCATCACGCACCAGGTCGCGGTGCAGCGGGATGTTGGTCTTGATGCCGTCG  >  NC_002947/648143‑648436                                                                                                                                                    |                                                                                                                                                      aTGCGGCACGGGCTTTGGGCATTTCGCGGGCGACGCTTTATCGGCGAGTGCATGAGCACCGCATCGACATGCCGCGGATGAAGGGGTAGAGCCCTGGGGGCCCCCCCCGGCCCCCAAATCTAGCAGACCAAAAAAAAAACCCGCCAAAg                                                                                                                                                      >  6:204685/1‑149 (MQ=255)            gCTTTGGGGATTTCGCGGGCGACGCTTTATCGGCGAGTGCATGAGCACCGCATCGACATGCCGCGGATGAAGGGGTAGAGCCCTGGTGCCGCGACGCGGCCCCAGATTCTGGCAG‑GCAATAAAAAACCCCGGCACAGGG‑CCGGGGTTCt                                                                                                                                         <  2:315039/149‑1 (MQ=255)             cTTTGGGCATTTCGCGGGCGACGCTTTATCGGCGAGTGCATGAGCACCGCATCGACATGCCGCGGATGAAGGGGTAGAGCCCTGGGGCCGCGACGCGGCCCCAGATTCTGGCAG‑GCAATAAAAAACCCCGGCACAGGG‑CCGGGGTTCTg                                                                                                                                        <  1:49289/149‑1 (MQ=255)                tGGGCATTTCGCGGGCGACGCTTTATCGGCGAGTGCATGAGCACCGCATCGACATGCCGCGGATGAAGGGGTAGAGCCCTGGGGCCGCGACGCGGCCCCAGATTCTGGCAG‑GCAATAAAAAACCCCGGCACAGGG‑CCGGGGTTCTgg                                                                                                                                       <  3:144073/147‑1 (MQ=255)                  ggCTTTTCGCGGGCGACGCTTTATCGGCGAGTGCATGAGCACCGCATCGACATGCCGCGGATGAAGGGGTAGAGCCCTGGGGCCGCGACGCGGCCCCAGATTCTGGCAG‑GCAATAAAAAACCCCGGCCCTGTG‑CCGGGGttttttatt                                                                                                                                    <  3:355003/148‑8 (MQ=255)                  ggCATTTCGCGGGCGACGCTTTATCGGCGAGTGCATGAGCACCGCATCGACATGCCGCGGATGAAGGGGTAGAGCCCTGGGGCCGCGCCCCGGCCCCACATTATGGGAG‑GCAATAAAAAAACCCCGCCAAGGG‑GCGGGGGTGTGGTTCa                                                                                                                                   >  6:318407/1‑149 (MQ=255)                   gCATTTCGCGGGCGACGCTTTATCGGCGAGTGCATGAGCACCGCATCGACATGCCGCGGATGAAGGGGTAGAGCCCTGGGGCCGCGACGCGGGCCCAGAGTGTGGCAG‑GCAATAAAAAACCCCGGCACAGGG‑GCGGGGTTCTGGTACa                                                                                                                                   >  2:227646/1‑148 (MQ=255)                    cATTTCGCGGGCGACGCTTTATCGGCGAGTGCATGACCACCGCATCGACATGCCGCGGATGAAGGGGTAGAGCCCTGGGGCCGCGACGCGGCCCCAGATTCTGGCAG‑GCAATAAAAAACCCCGGCACAGGG‑CCGGGGTTCTGGTACATc                                                                                                                                 <  5:223039/149‑1 (MQ=255)                     aTTTCGCGGGCGACGCTTTATCGGCGAGTGCATGAGCACCGCATCGACATGCCGCGGATGAAGGGGTAGAGCCCTGGGGCCGCGACGCGGCCCCAGATTCTGGCAG‑GCAATAAAAACCCCCGGCACAGGG‑CCGGGGTTCTGGTACATca                                                                                                                                <  1:227646/149‑1 (MQ=255)                                   gCTTTATCGGCGAGTGCATGAGCACCGCATCGACATGCCGCGGATGAAGGGGTAGAGCCCTGGGGGCGCGACCCGGCCCCCCATTTTGGGCG‑GGAAAAAAAAAACCCCGCACACGG‑CCGGGGGTGTGGTAAAAAACACAAAACGaaaa                                                                                                                   >  2:57582/1‑145 (MQ=255)                                   gCTTTATCGGCGAGTGCATGAGCACCGCATCGACATGCCGCGGATGAAGGGGTAGAGCCCTGGGGCCGCGACGCGGCCCCAGATTCTGGCAG‑GCAATAAAAAACCCCGGCACAGGG‑CCGGGGTTCTGGTACATCACACTACACGATCAc                                                                                                                  <  1:61130/149‑1 (MQ=255)                                             cGAGTGCATGAGCACCGCATCGACATGCCGCGGATGAAGGGGTAGAGCCCTGGGGCCGCGCCGCGGCCCCAGATTGTGGGAG‑GGAAAAAAAAAACCCGGCCCAGGG‑GCGGGGTTCTGGTACATCACACTAAACCATCAcac                                                                                                                >  6:277409/1‑139 (MQ=255)                                             cGAGTGCATGAGCACCGCATCGACATGCCGCGGATGAAGGGGTAGAGCCCTGGGGCCGCGACGCGGCCCCAGATTCTGGCAG‑GCAATAAAAAACCCCGGCACAGGGCCCGGGGTTCTGGTACATCACACTACACGATCACTCCTGGTTg                                                                                                         <  1:251775/149‑1 (MQ=255)                                             cGAGTGCATGAGCACCGCATCGACATGCCGCGGATGAAGGGGTAGAGCCCTGGGGCCGCGACGCGGCCCCAGATTCTGGCAG‑GCAATAAAAAACCCCGGCACAGGG‑CCGGGGTTCTGGTACATCACACTACACGATCACTc                                                                                                                <  5:277409/141‑1 (MQ=255)                                                        gaaCCTCATCGACATGCCGCGGATTAAGGGGTAGAGCCCTGTGGCCGCGACGCGGCCCCAGATTCTGGCAG‑GCAATAAAAAACCCCGGCACAGGG‑CCGGGGTTCTGGTACATCACACTACACGATCACTCCTGGTTGGCCAGTTTGTGc                                                                                             <  5:204685/147‑1 (MQ=255)                                                           ccGCATCGACATGCCGCGGATGAAGGGGTAGAGCCCTGGGGCCCCGACGCCGCCCCAAATTAATGCAA‑GCAATAAAAAAACCCCGCACACGG‑CCGGGGGTATGGTAAAACACAAAACACCAAAACACATGCTTGGACACTTTGTGGTc                                                                                           >  4:282750/1‑148 (MQ=255)                                                                        ccGCGGATGAAGGGGTAGAGCCCTGGGGCCGCGACGCGGCCCCAGATTCTGGCAG‑GCAATAAAAAACCCCGGCACAGGG‑CCGGGGTTCTGGTACATCACACTACACGATCACTCCTGGTTGGCCAGTTTGTGCTCGAGGTAGTGAATGt                                                                             <  5:318407/149‑1 (MQ=255)                                                                            ggATGAAGGGGTAGAGCCCTGGGGCCGCGACGCGGCCCCAGATTCTTGCAG‑GCAATAAAAAACCCCGGCACAGGG‑CCGGGGTTCTGGTACATCACACTACACGATCACTCCTGTTTGGCCAGTTTGTGCTCGAGGTAGTGAATGTTGAc                                                                         <  3:51286/149‑1 (MQ=255)                                                                                     ggTAGAGCCCTGGGGCCGCGACGCGGCCCCAGATTCTGGCAG‑GCAATAAAAAACCCCGGCACAGGG‑CCGGGGTTCTGGTACATCACACTACACGATCACTCCTGGTTGGCCAGTTTGTGCTCGAGGTAGTGAATGTTGACGCCGCCttt                                                                <  1:57582/149‑1 (MQ=255)                                                                                      gTAGAGCCCTGGGGCCGCGACGCGGCCCCAGATTCTGGCAG‑GCAATAAAAAACCCCGGCACAGGG‑CCGGGGTTCTGGTACATCACACTACACGATCACTCCTGGTTGGCCAGTTTGTGCTCGAGGTa                                                                                     <  5:337421/127‑1 (MQ=255)                                                                                           gCCCTGGGGCCGCGACGCGGCCCCAGATGATGGCAG‑TCAATAAAAAACCCCGGCACAGGG‑CCGGGGTTCTGGTACATCACACTACACGATCACTCCTGGTTGGCCAGTTTGTGCTCGAGGTAGTGAATGTTGACGCCGCCTTTGCAGaa                                                          <  3:282750/149‑1 (MQ=255)                                                                                                ggggCCGCGACGCGGCCCCAGATTCTGGCAG‑GCAATAAAAAACCCCGGCACAGGG‑CCGGGGTTCTGGTACATCACACTACACGATCACTCCTGGTTGGCCAGTTTGTGCTCGAGGTAGTGAATGTTGACGCCGCCTTTGCAGAAG‑CCtt                                                    >  5:143708/1‑149 (MQ=255)                                                                                                     cgcgACGCGGCCCCAGATTCTGGCAG‑GCAATAAAAAACCCCGGCACAGGG‑CCGGGGTTCTGGTACATCACACTACACGATCACTCCTGGTTGGCCAGTTTGTGCTCGAGGTAGTGAATGTTGAcgcc                                                                      >  7:103196/1‑127 (MQ=255)                                                                                                     cgcgACGCGGCCCCAGATTCTGGCAG‑GCAATAAAAAACCCCGGCACAGGG‑CCGGGGTTCTGGTACATCACACTACACGATCACTCCTGGTTGGCCAGTTTGTGCTCGAGGTAGTGAATGTTGAcgcc                                                                      <  8:103196/127‑1 (MQ=255)                                                                                                       cgACGCGGCCCCAGATTCTGGCAG‑GCAATAAAAAACCCCGGCACAGGG‑CCGGGGTTCTGGTACATCACACTACACGATCACTCCTGGTTGGCCAGTTTGTGCTCGAGGTAGTGAATGTTGACGCCGCCTTTGCAGAAG‑CCTTCATCACg                                             <  1:336387/149‑1 (MQ=255)                                                                                                           gcgGCCCCAGATTCTGGCAG‑GCAATAAAAAACCCCGGCACAGGG‑CCGGGGTTCTGGTACATCACACTACACGATCACTCCTGGTTGGCCAGTTTGTGCTCGAGGTAGTGAATGTTGACGCCGCCTTTGCAGAAG‑CCTTCATCACGCAc                                          >  8:36448/1‑148 (MQ=255)                                                                                                             ggCCCCAGATTCTGGCAG‑GCAATAAAAAACCCCGGCACAGGG‑CCGGGGTTCTGGTACATCACACTACACGATCACTCCt                                                                                                              >  5:282291/1‑79 (MQ=255)                                                                                                             ggCCCCAGATTCTGGCAG‑GCAATAAAAAACCCCGGCACAGGG‑CCGGGGTTCTGGTACATCACACTACACGATCACTCCt                                                                                                              <  6:282291/79‑1 (MQ=255)                                                                                                                cccAGATTCTGGCAG‑GCAATAAAAAACCCCGGCACAGGG‑CCGGGGTTCTGGTACATCACACTACACGATCACTCCTGGTTGGCCAGTTTGTGCTCGAGGTAGTGAATGTTGACGCCGCCttt                                                                >  1:42697/1‑122 (MQ=255)                                                                                                                cccAGATTCTGGCAG‑GCAATAAAAAACCCCGGCACAGGG‑CCGGGGTTCTGGTACATCACACTACACGATCACTCCTGGTTGGCCAGTTTGTGCTCGAGGTAGTGAATGTTGACGCCGCCttt                                                                <  2:42697/122‑1 (MQ=255)                                                                                                                  cAGATTCTGGCAG‑GCAATAAAAAACCCCGGCACAGGG‑CCGGGGTTCTGGTACATCACACTACACGATCACTCCTGGTTGGCCAGTTTGTGCTCGAGGTAGTGAATGTTGACGCCGCCTTTGCAGAAG‑CCTTCATCACGCACCAGGTcgc                                  >  3:294118/1‑149 (MQ=255)                                                                                                                   aGATTCTGGCAG‑GCAATAAAAAACCCCGGCACAGGG‑CCGGGGTTCTGGTACATCACACTACACGATCACTCCTGGTTGGCCAGTTTGTGCTCGAGGTAGTGAATGTTGACGCCGCCTTTGCAGAAG‑CCTTCATCACGCACCAGGTcgcg                                 <  7:275961/149‑1 (MQ=255)                                                                                                                     aTTCTGGCAG‑GCAATAAAAAACCCCGGCACAGGG‑CCGGGGTTCTGGTACATCACACTACACGATCACTCCTGGTTGGCCAGTTTGTGCTCGAGGTAGTGAATGTTGACGCCGCCTTTGCAGAAG‑CCTTCATCACGCACCAGGTCGCGGt                               <  7:36448/149‑1 (MQ=255)                                                                                                                      ttCTGGCAG‑GCAATAAAAAACCCCGGCACAGGG‑CCGGGGTTCTGGTACATCACACTACACGATCACTCCTGGTTGGCCAGTTTGTGCTCGAGGTAGTGAATGTTGACGCCGCCTTTGCAGAAG‑CCTTCATCACGCACCAGGTcgc                                  <  1:224445/145‑1 (MQ=255)                                                                                                                      ttCTGGCAG‑GCAATAAAAAACCCCGGCACAGGG‑CCGGGGTTCTGGTACATCACACTACACGATCACTCCTGGTTGGCCAGTTTGTGCTCGAGGTAGTGAATGTTGACGCCGCCTTTGCAGAAG‑CCTTCATCACGCACCAGGTcgc                                  >  2:224445/1‑145 (MQ=255)                                                                                                                         tGGCAG‑GCAATAAAAAACCCCGGCACAGGG‑CCGGGGTTCTGGTACATCACACTACACGATCACTCCTGGTTGGCCAGTTTGTGCTCGAGGTAGTGAATGTTGACGCCGCCTTTGCAGAAG‑CCTTCATCACGCACCAGGTCGCGGTGCAg                           >  4:175685/1‑149 (MQ=255)                                                                                                                         tGGCAG‑GCAATAAAAAACCCCGGCACAGGG‑CCGGGGTTCTGGTACATCACACTACACGATCACTCCTGGTTGGCCAGTTTGTGCTCGAGGTAGTGAATGTTGACGCCGCCTTTGCAGAAG‑CCTTCATCACGCACCAGGTCGCGGTGCAg                           >  5:323163/1‑149 (MQ=255)                                                                                                                                  gtaccagaaCCCCGGCCCTGTG‑CCGGGGTTCTGGTACATCACACTACACGATCACTCCTGGTTGGCCAGTTTGTGCTCGAGGTAGTGAATGTTGACGCCGCCTTTGCAGAAG‑CCTTCATCACGCACCAGGTCGCGGTGCAGCGGGATGt                   >  7:111754/8‑149 (MQ=255)                                                                                                                                  aaTAAAAAACCCCGGCACAGGG‑CCGGGGTTCTGGTACATCACACTACACGATCACTCCTGGTTGGCCAGTTTGTGCTCGAGGTAGTGAATGTTGACGCCGCCTTTGCAGAAG‑CCTTCATCACGCACCAGGTCGCGGGGCAGCGGGATGt                   >  7:247454/1‑149 (MQ=255)                                                                                                                                    tAAAAAACCCCGGCACAGGG‑CCGGGGTTCTGGTACATCACACTACACGATCACTCCTGGTTGGCCAGTTTGTGCTCGAGGTAGTGAATGTTGACGCCGCCTTTGCAGAAG‑CCTTCATCACGCACCAGGTCGCGGTGCAGCGGGATGTTg                 <  3:175685/149‑1 (MQ=255)                                                                                                                                    tAAAAAACCCCGGCACAGGG‑CCGGGGTTCTGGTACATCACACTACACGATCACTCCTGGTTGGCCAGTTTGTGCTCGAGGTAGTGAATGTTGACGCCGCCTTTGCAGAAG‑CCTTCATCACGCACCAGGTCGCGGTGCAGCGGGATGTTg                 <  7:42806/149‑1 (MQ=255)                                                                                                                                     aaacaaCCCCGGCACAGGG‑CCGGGGTTCTGGTACATCACACTACACGATCACTCCTGGTTGGCCAGTTTGTGCTCGAGGTAGTGAATGTTGACGCCGCCTTTGCAGAAG‑CCTTCATCACGc                                            <  8:234346/121‑1 (MQ=255)                                                                                                                                     aaaaaaCCCCGGCACAGGG‑CCGGGGTTCTGGTACATCACACTACACGATCACTCCTGGTTGGCCAGTTTGTGCTCGAGGTAGTGAATGTTGACGCCGCCTTTGCAGAAG‑CCTTCATCACGc                                            >  7:234346/1‑121 (MQ=255)                                                                                                                                     aaaaaaCCCCGGCACAGGG‑CCGGGGTTCTGGTACATCACACTACACGATCACTCCTGGTTGGCCAGTTTGTGCTCGAGGTAGTGAATGTTGACGCCGCCTTTGCAGAAG‑CCTTCATCACGCACCAGGTCGCGGTGCAGCGGGATGTTgg                >  8:296355/1‑149 (MQ=255)                                                                                                                                      aaaaaCCCCGGCACAGGG‑CCGGGGTTCTGGTACATCACACTACACGATCACTCCTGGTTGGCCAGTTTGTGCTCGAGGTAGTGAATGTTGACGCCGCCTTTGCAGAAG‑CCTTCATCACGCACCAGGTCGCGGTGCAGCGGGATGTTGGt               >  3:44089/1‑149 (MQ=255)                                                                                                                                        aaaCCCCGGCACAGGG‑CCGGGGTTCTGGTACATCACACTACACGATCACTCCTGGTTGGCCAGTTTGTGCTCGAGGTAGTGAATGTTGACGCCGCCTTTGCAGAAG‑CCTTCATCACGCACCAGGTCGCGGTGCAGCGGGATGTTGGTCt             >  8:126134/1‑149 (MQ=255)                                                                                                                                         aaCCCCGGCACAGGG‑CAGGGGTTCTGGTACATCACACTACACGATCACTCCTGGTTGGCCAGTTTGTGCTCGAGGTAGTGAATGTTGACGCCGCCTTTGCTGGGGTCCTTCATCACGCACCAGGTCGCGGTGCAGCGGGATGTTGGTCt             >  1:119961/1‑149 (MQ=255)                                                                                                                                          aCCCCGGCCCTGTG‑CCGGGGTTCTGGTACATCACACTACACGATCACTCCTGGTTGGCCAGTTTGTGCTCGAGGTAGTGAATGt                                                                             >  7:160133/1‑84 (MQ=39)                                                                                                                                          aCCCCGGCCCTGTG‑CCGGGGTTCTGGTACATCACACTACACGATCACTCCTGGTTGGCCAGTTTGTGCTCGAGGTAGTGAATGt                                                                             <  8:160133/84‑1 (MQ=39)                                                                                                                                            cccGGCACAGGG‑CCGGGGTTCTGGTACATCACACTACACGATCACTCCTGGTTGGCCAGTTTGTGCTCGAGGTAGTGAATGTTGACGCCGCCTTTGCAGAAG‑CCtt                                                    >  7:182074/1‑106 (MQ=255)                                                                                                                                            cccGGCACAGGG‑CCGGGGTTCTGGTACATCACACTACACGATCACTCCTGGTTGGCCAGTTTGTGCTCGAGGTAGTGAATGTTGACGCCGCCTTTGCAGAAG‑CCtt                                                    <  8:182074/106‑1 (MQ=255)                                                                                                                                                   caGGG‑CCGGGGTTCTGGTACATCACACTACACGATCACTCCTGGTTGGCCAGTTTGTGCTCGAGGTAGTGAATGTTGACGCCGCCTTTGCAGAAG‑CCTTCATCACGCACCAGGTCGCGGTGCAGCGGGATGTTGGTCTTGATGCCGTcg  <  7:126134/149‑1 (MQ=255)                                                                                                                                                    aGGG‑CCGGGGTTCTGGTACATCACACTACACGATCACTCCTGGTTGGCCAGTTTGTGCTCGAGGTAGTGAATGTTGACGCCGCCTTTGCAGAAG‑CCTTCATCACGCACCAGGTCGCGGTGCAGCGGGATGTTGGt               >  3:35834/1‑135 (MQ=255)                                                                                                                                                    aGGG‑CCGGGGTTCTGGTACATCACACTACACGATCACTCCTGGTTGGCCAGTTTGTGCTCGAGGTAGTGAATGTTGACGCCGCCTTTGCAGAAG‑CCTTCATCACGCACCAGGTCGCGGTGCAGCGGGATGTTGGt               <  4:35834/135‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                      ATGCGGCACGGGCTTTGGGCATTTCGCGGGCGACGCTTTATCGGCGAGTGCATGAGCACCGCATCGACATGCCGCGGATGAAGGGGTAGAGCCCTGGGGCCGCGACGCGGCCCCAGATTCTGGCAG‑GCAATAAAAAACCCCGGCACAGGG‑CCGGGGTTCTGGTACATCACACTACACGATCACTCCTGGTTGGCCAGTTTGTGCTCGAGGTAGTGAATGTTGACGCCGCCTTTGCAGAAG‑CCTTCATCACGCACCAGGTCGCGGTGCAGCGGGATGTTGGTCTTGATGCCGTCG  >  NC_002947/648143‑648436

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑